Life Sciences Commons^™

Using An Old Assay In New Ways: Differential Scanning Calorimetry Characterization, Quantification, And Interaction Analysis Of Whey Proteins, Charity Conlin Jennings

Theses and Dissertations

Whey proteins including β-Lactoglobulin, α-Lactalbumin, Immunoglobulin-G, Serum Albumin, Lactoferrin, and Lactoperoxidase each have unique biological properties spanning anti-bacterial, anti-fungal, and anti-viral activities as well as deliver essential nutrients to the human body. In the past decade, there has been a large focus on extracting these individual proteins for their use in foods and supplements. In order to best extract these proteins and prepare them properly in foods and supplements, an understanding of how they behave and denature in different environments is necessary. However, there is insufficient knowledge of their thermodynamic properties and potential interactions in varying conditions. Additionally, there are …

Adipokines Mediate Fat-To-Ovary Communication To Regulate Oogenesis, Adipocyte Size, And Lipid Storage In Drosophila Melanogaster, Chad Herbert Simmons

Theses and Dissertations

Humans have complex signaling pathways that allow them to store and expend energy based on nutritional quality and availability. Inter-organ signaling is used to communicate nutrient availability between organs based on nutritional needs and the rate of cellular division. Our main overarching question is to investigate how fat-derived signals affect adipocyte morphology and the maintenance of stem cells in the ovary. While signaling pathways, like insulin and mTOR, and some adipokines that are involved in inter-organ nutrient sensing have been identified, the exact nutrient reliant mechanisms of stem cell maintenance are not completely understood. Using Drosophila melanogaster as a model, …

Investigation Of Metals And Incidental Nanomaterials In Wildland-Urban Interface Fire Ashes And Their Impacts On Marine Phytoplankton, Talal Abdullah Alshehri

Theses and Dissertations

Wildfires are natural processes that have played a vital role in ecosystems for millions of years. However, with increasing anthropogenic activities and climate change, wildfires' frequency and intensity have increased. A wildfire is an uncontrolled fire that burns forests, grasses, and savannas. In addition to vegetation combustible materials, wildfire burn structure items such as homes, stores, and storage sheds in areas where the transition zone between urban and wildland is defined as the wildland-urban interface (WUI). Moreover, wildfires in WUI can burn vehicles, including automobiles, tractors, and boats on trails. Recently, there have been concerns regarding the metals, metalloids, and …

Exploring The Diversity And Biology Of Unique Giant Broad Host Range Bacteriophages In Natural Environments, Tannaz Mohammadi

Theses and Dissertations

Microbial interactions within the rhizosphere play a crucial role in shaping bacterial community diversity and function. To explore these dynamics, we isolated 21 bacterial strains from soil and plant root samples collected near Rocky Branch Creek on the University of South Carolina campus. Our findings revealed that a significant proportion of these bacteria are lysogenic for CbK-like phages belonging to the Dolichocephalovirinae subfamily. Additionally, we isolated free bacteriophages directly from the same soil samples and found them to be closely related to those harbored within lysogenic bacteria. Surprisingly, despite predictions of a narrow host range, most of these phages, both …

Unveiling Caulocovert: Conserved Phage And Gene Transfer Agent In Caulobacter, Talia Neco

Theses and Dissertations

Bacteriophage play an important role in bacterial evolution, population control, and horizontal gene transfer within microbial ecosystems. This study focuses on the discovery and characterization of Caulocovert, an active bacteriophage identified in multiple Caulobacter strains. Using genome annotation tools such as Artemis and Mauve, we identified multiple key phage genes, including the major capsid protein, head-tail connector, portal protein, and major tail protein. These phage genes are present in CB13 and several other strains, such as C. vibriodies CB15 and CB2, C. segnis CBR1, Caulobacter sp. FWC26, and the distantly related Caulobacter sp. D5 strain. The location of these genes …

Evaluating The Performance And Feasibility Of A Device-Agnostic Approach To Compare Raw Accelerometry And Hr Data From Consumer Wearables To Research Grade Devices In Young Adults, Candy Bryan Mrudula Yalla

Theses and Dissertations

INTRODUCTION: Wearable technologies are increasingly used to monitor physical activity and heart rate in both research and everyday settings. This study aimed to compare accelerometery and heart rate (HR) data from two consumer wearables Apple Watch Series 7 and Google Pixel Watch Series 2, with research-grade devices: the ActiGraph GT9X Link and ActiHeart 5.

METHODS: Twenty-four young adults aged 18–24 years (mean age = 22.4; 54.2% female) participated in a 90-minute activity protocol while wearing the devices. The protocol included structured tasks ranging from rest to vigorous intensity. Consumer wearables and ActiGraph were worn on the non-dominant wrist, and the …

Multiscale Modelling Of Eg5: Structural Insights Into Microtubule Interactions, Atp Hydrolysis, And Allosteric Inhibition Mechanisms, Soundarya Priya Alexandar Ms

Theses and Dissertations

The mitotic kinesin EG5 is essential for spindle bipolarity and accurate chromosome segregation during cell division, making it a validated anti-cancer target. Unlike conventional microtubule-targeting agents, EG5 inhibitors offer mitosis-specific action, reducing neurotoxicity. Although inhibitors targeting EG5’s allosteric site-I have been extensively studied, mechanistic insights into inhibition at site-II, and their combined effects, remain underexplored. This thesis systematically investigates the structural and dynamic mechanisms underlying EG5 function and its inhibition using an integrative computational approach.

Conformational analysis, coarse-grained dynamics, all-atom molecular dynamics simulations, and protein network analysis were performed on EG5 motor domain structures and EG5-microtubule complexes. Results show that …

Functional Role Of Cysteine Residues In Vtlr Of Agrobacterium Tumefaciens, Suravi Islam

Theses and Dissertations

Agrobacterium tumefaciens is a soil-dwelling plant pathogen known for transferring DNA to host plants through a type IV secretion system. Central to its virulence and cellular regulation is the LysR-type transcriptional regulator, VtlR, which controls processes including biofilm formation, motility, tumorigensis, and small RNA expression. Although VtlR’s regulatory role is established, the specific functions of its conserved cysteine residues have only recently begun to be explored. This study investigates VtlR’s four cysteines (C144, C236, C244, and C273) through site-directed mutagenesis and a combination of phenotypic assays and transcriptional profiling. Phenotypic assays confirmed that C144 and C273 are important for proper …

Ground-Based Modeling Of Deep-Space Risks To The Spleen And Intestine In Mice, Edith Nathalie Pineda Sotomayor

Theses and Dissertations

Deep-space missions, such as those planned for the cis-lunar space and Mars, pose unique challenges to astronaut health due to prolonged microgravity (MG) exposure and low-dose chronic irradiation (CIR). Understanding the impact of these stressors on secondary lymphoid tissues, such as the spleen and intestine, is crucial for ensuring crewmembers' well-being, given the impracticality of conducting this research with human samples. Using a ground-based facility, we exposed C57BL/6 female and male mice to CIR (1.74 Gy at a dose rate of 0.0029 Gy/h) and/or simulated MG (SMG) via hind limb unloading (HLU) for one month. Spleen and intestinal tissues were …

Analyzing The Role Of A Dual-Function Diguanylate Cyclase / Phosphodiesterase For Attachment And Motility In Agrobacterium Tumefaciens, Priya Aryal

Theses and Dissertations

Cyclic diguanylate monophosphate (c-di-GMP) is a second messenger that is responsible for attachment and motility in many prokaryotic systems and controls the signaling pathway for the motile to sessile transition in many bacteria. Many c-di-GMP-metabolizing proteins are diguanylate cyclases (DGCs), responsible for the synthesis of c-di-GMP and characterized by a c-di-GMP synthesizing domain, e.g., GGDEF domain. Other c-di-GMP-metabolizing proteins contain c-di-GMP degradative domains, e.g., EAL, characterized by phosphodiesterase activity (PDE). In Agrobacterium tumefaciens, out of 31 predicted proteins regulating the c-di-GMP level, this project focuses on the predicted dual-function DGC/PDE, DcpB. We hypothesized that DcpB plays a role in the …

Examining The Role Of The M1 Motif Of Abcr1 Srna In Agrobacterium Tumefaciens, Daniel Arango-Cardona

Theses and Dissertations

Agrobacterium tumefaciens is a gram-negative alphaproteobacterium that is one of the causative agents of crown gall tumors on plants. Its ability to transform plants using its T-DNA has been studied to improve plant-mediated transformation. sRNAs are small regulatory RNAs that play a role in gene expression in a variety of organisms and bind to target proteins or mRNAs. A. tumefaciens contains two sibling sRNAs, called AbcR1 and AbcR2, which are conserved among alphaproteobacteria. A. tumefaciens AbcR1 and AbcR2 are closely related, exhibiting 69% sequence identity, similar secondary and tertiary structure, and a conserved motif, M2, believed to direct target interactions. …

An Antioxidant Approach To Ameliorate The Effects Of Radiation And Chemotherapy On Cognition, Raymond Anan Otoo

Theses and Dissertations

Acute Lymphoblastic Leukemia (ALL) is a hematologic malignancy that primarily affects children and young adults. While advancements in treatment—including chemotherapy and cranial irradiation—have significantly improved 5-year survival rates in children to nearly 90%, radiation-induced cognitive impairment (RICI) remains a persistent and debilitating side effect. The brain, particularly the hippocampus, is highly susceptible to radiation-induced oxidative stress and inflammation. In the treatment of ALL, the combined use of chemotherapy and radiotherapy contributes to a heightened oxidative burden, exacerbating the risk of cognitive dysfunction. Although previous studies have linked radiation exposure to neurocognitive decline, the molecular mechanisms underlying RICI and chemotherapy-induced cognitive …

Exposure To Zinc Oxide Nanoparticles Reveals A Death Resistance Mechanism In Dictyostelium Discoideum, Zoya Nadeem Faruqui

Theses and Dissertations

Zinc oxide nanoparticles (ZnO NPs) are very versatile due to their multifunctional properties including unique physicochemical and antimicrobial characteristics. They are widely utilized across various fields such as biomedical, industrial fields, environmental remediation, and agriculture. Therefore, their cytotoxic effects on biological systems necessitate a deeper understanding of the cellular responses to nanoparticle exposure. This project primarily investigates the impact of ZnO NPs on the model organism Dictyostelium discoideum AX4 (social amoeba) which has not been examined before unlike a vast variety of organisms in the literature. The cytotoxic effects of ZnO NPs on Dictyostelium discoideum AX4 cells are compared with …

In Silico Investigations Of Class Ii Trans Activator (Ciita): Its Plausible Implications In Neuroinflammation And Neurological Disorders, Kishore N

Theses and Dissertations

Inflammation plays a significant role in maintaining homeostasis. Neuroinflammation is a chronic inflammatory response in the central nervous system (CNS), leading to brain or spinal cord dysfunction. To promote mental health, it is crucial to understand the regulation of neuroinflammation. Class II transactivator (CIITA) is a master-regulator of the major histocompatibility complex II (MHCII).

Modulation of CIITA has been suggested as a promising intervention for inflammatory conditions, including neurological disorders. However, there is limited knowledge about the role of CIITA and its interactors in inflammation. This study aimed to understand the role of CIITA in neuroinflammation and neurological disorders using …

3d Bioprinting On Electrospun Nanofibrous Scaffolds To Fabricate Myocardial Patches, Muthu Parkkavi S

Theses and Dissertations

Tissue-engineered three-dimensional (3D) myocardial patches offer superior and innovative treatment strategy for myocardial infarction (MI). Among the various scaffold fabrication techniques, electrospinning and 3D bioprinting have received great attention due to their ability to recreate native cardiac tissue mimetic characteristics. Despite several advantages, electrospun nanofibers have limitations in cell delivery due to random cell seeding, while bioprinting faces challenges in creating hydrogel constructs with ideal mechanical properties capable of supporting cardiac contraction/relaxation cycle. Hence in this work, hybrid myocardial patches were developed by converging electrospinning and bioprinting techniques.

Electrospinning of PLCL:PEOz (B73, 7:3 blend of poly-L-lactide-co-ε-caprolactone (PLCL) and polyethyl oxazoline …

Effect Of Osmolytes On The Interplay Of Water Protein And Dna In Ecori Dna Recognition Using Molecular Dynamics Simulations, Aathithya D

Theses and Dissertations

EcoRI is a type II restriction endonuclease that has been widely used as a model system to study protein-DNA interaction owing to its high degree of specificity. This enzyme does not recognize even a single basepair change in its recognition sequence, (GAATTC)2, under normal conditions. However, in the presence of osmolytes, such as glycerol and DMSO, the specificity of EcoRI is slightly relaxed and recognizes sequences that differ from its cognate sequence in the first basepair position. This relaxed specificity has been attributed to the dehydration of the EcoRI-DNA which presumably results in tighter complex formation and subsequent catalysis. However, …

Biogenic Calcite Precipitation For Coastal Sand Stabilization: A Synergistic Approach Using Marine Bacteria And Natural Fibers, Aparna Gs

Theses and Dissertations

Coastal erosion, driven by natural forces such as waves, tides, winds, and currents and further intensified by anthropogenic activities like urbanization, climate change, and coastal infrastructure development, poses a significant threat to shoreline stability. Conventional erosion control methods, including jetties, embankments, and artificial reefs, often face limitations in sustainability and effectiveness. In this context, microbially induced calcite precipitation (MICP) has emerged as a promising ecofriendly solution for ground stabilization.

The present study investigates the potential of an indigenous ureolytic marine bacterium, Bacillus sp. N₉, isolated from the coastal sediments of Nagapattinam, Tamil Nadu, for coastal sand stabilization. The strain was …

Fabrication Of Protein-Based Nanomicelle Against Inflamed Synoviocytes In Rheumatoid Arthritis, Madhumithra Thangadurai

Theses and Dissertations

Rheumatoid arthritis (RA) is a chronic autoimmune disease marked by persistent synovial inflammation, progressive cartilage and bone destruction, and systemic complications. 1% of the global population are affected by RA, when their immune system targets synovial tissues, leading to joint pain, swelling, and functional disability. Standard treatments, such as disease-modifying antirheumatic drugs (DMARDs) like methotrexate (MTX), are limited by systemic toxicity and inconsistent patient responses, highlighting the need for improved therapies. This research work explores protein-glycosaminoglycan nanomicelles engineered using Zein with Chondroitin or carboxymethylated Dextran Sulfate as targeted drug delivery vehicles for RA.

MTX was encapsulated within these nanomicelles to …

Genomic Surveillance Of Indian Klebsiella Pneumoniae And Exploration Of Resistance Inhibitors, Abhirami Ks Ms

Theses and Dissertations

Klebsiella pneumoniae is an important ESKAPE pathogen that causes life threatening infections. The strains exhibiting multidrug-resistance (MDR) and hypervirulence are the priority pathogens for which immediate treatment and dissemination prevention strategies are required. To address this, genome profiling was performed to understand the AMR patterns, role of episomes, phages, virulence determinants and its association with sequence types.

The study includes 267 publicly available Indian K. pneumoniae genomes over a period of ten years and four sequenced isolates. Whole genome analysis revealed the widespread presence of diverse β-lactamase genes, with β-lactam resistance being predominant. Genes such as blaCTX-M-15, blaTEM-1B and blaOXA-232 …

Remote Running Gait Analysis Using Smartphone Video And Monocular Pose Estimation: A Feasibility Study, Luke Vankeersbilck

Theses and Dissertations

Introduction: Running is a common form of exercise that comes with many health benefits; however, many people stop exercising because of the high injury risk. Running injury is connected with biomechanical risk factors. Yet, these risk factors often show inconsistent and conflicting results across studies, largely due to small sample sizes limiting statistical power. Low subject numbers may be partly due to the time requirements of traditional motion capture. Monocular pose estimation offers a promising alternative by using machine learning to track body landmarks from a single camera. This study evaluated the feasibility of remotely collecting running biomechanics data using …

Circadian Influences On Glycemic Control In 24-Hr Fasts Initiated At Different Mealtimes, Elizabeth Z. Gipson

Theses and Dissertations

This study investigated whether the timing of a 24-hour fast influences indicators of glycemic control throughout the duration of the fast or upon refeeding. Twenty-four adults who have overweight or obesity (13 female, 11 male) completed this randomized crossover study involving three 24-hour fasts initiated at either 8:00 am, 1:00 pm, or 6:00 pm followed by a 75 g oral glucose tolerance test (OGTT) which lasted 2 hours. Continuous glucose monitors (CGMs) were employed to track interstitial glucose throughout each fast and OGTT. Plasma samples for insulin, glucagon, glucagon-like peptide-1 (GLP-1), and glucose-dependent insulinotropic polypeptide (GIP) were collected at the …

Chemically Mediated Communication In Mound-Building Termites, Sree Subha Ramaswamy

Theses and Dissertations

Nest-building in social insects requires layered communication to coordinate complex, functional structures for housing, protection, storage, and homeostasis. This is especially striking in mound-building termites, which construct and repair large mounds collectively despite lacking image-forming eyes. This thesis investigates how Odontotermes obesus achieves such coordination through chemical communication embedded in soil.

Using controlled two-choice assays with manipulated soils, I separated airborne volatile signals from contact non-volatile signals by baking to remove volatiles, a mesh assay that presented volatile cues without contact, and solvent extraction with reapplication of contact cues. I quantified responses in major-only, minor-only, and mixed groups at two …

Developing Site-Specific Water Response Functions Using Historical Soil Moisture Data, Gifty Lad Ayela

Theses and Dissertations

Variable rate irrigation (VRI) systems offer a promising solution by optimizing water application based on site-specific needs in different areas within a crop field. While accurate crop water production functions (CWPFs) are essential for VRI systems, developing CWPFs has been hindered by challenges in data collection and the complexities of modeling water’s impact on yields. This study attempts to develop site-specific CWPFs using farmers’ historical soil moisture data, collected using soil water tension (SWT) as a proxy, and yield maps. The spatial variability of the crop-water response functions was modeled using Geographically Weighted Regression (GWR) and the results from this …

Transcription Fidelity In The Face Of Oxidative Damage, Eastilan Gyiwah Appiah

Theses and Dissertations

It is very important that the process of transcription is correctly carried out in the cell for the faithful expression of the genetic code. However, should mistakes happen, they can result in transcription errors which interfere with the protein-making machinery of the cell. This will result in accumulation of proteins and dysfunctional proteins that have the potential of causing diseases like Alzheimer’s. Oxidative damage caused by hydrogen peroxide is known to cause transcription errors in organisms like yeast. It is therefore important to investigate the extent to which hydrogen peroxide can damage cells.

Influence Of Diverse Forage Environments On Honey Bee Colony Health, Angus Catchot Iii

Theses and Dissertations

Honey bees are important pollinators that face a multitude of stressors in their environment such as pathogens, parasite pressure, forage availability and pesticide exposure. This study examined how the makeup of different environments and the stressors related to those environments are affecting honey bee health in Mississippi. A field experiment was conducted during the first year of the study across four distinct landscapes ranging from intensively cultivated cropland to predominantly natural forage habitats to assess the impact of landscape availability on varying honey bee colony health parameters. Locations that had cultivated crops within foraging range produced more honey and brood …

Effects Of Psychological Arousal And Relaxation On Muscle Performance, Activation, And Heart Rate Variability During Maximal Muscle Strength And Precision Tasks, Allison Ann Brown

Theses and Dissertations

Psychological arousal plays a vital role in athletic performance, with the optimal point of arousal and performance level varying by task complexity. A common manipulator of arousal is music, which can produce multiple physiological effects. This study investigated whether unfamiliar arousing or relaxing music influences heart rate variability, muscle activation, maximal muscle strength, and muscle precision. Theoretically, if participants are found to have differing muscle performance responses as a result of differing music selections, this would further support the notion that the optimal point of arousal and performance is dependent upon the complexity of the task at hand. Thus, coaches …

Strategic Use Of Exogenous Feed Enzymes And Sodium Bisulfate To Optimize Broiler Production, Spencer Thomas Karn

Theses and Dissertations

Poultry producers must continually evaluate nutritional strategies to enhance broiler performance and sustainability. Chapter II assessed sodium bisulfate (SB) applied to litter, feed, and water across a 42-day grow-out. Applying SB to litter reduced pH, while 0.3% and 0.4% dietary SB improved feed conversion ratio (FCR). Water acidification with SB during the final week did not impair performance, supporting its use as a preharvest intervention. Chapter III evaluated phytase at 500, 1500, and 2000 FTU/kg, with or without protease, in reduced amino acid diets over 56 days. Birds fed a nutrient-adequate diet with 1500 FTU/kg phytase outperformed all reduced-amino acid …

From Gametes To Genes: Genetic Quality Assessment Of Anuran Cryopreserved Spermatozoa And Derived Embryos, Trinity Isabella Kassuhn

Theses and Dissertations

Sperm cryopreservation is an important tool within the suite of assisted reproductive technologies (ART) as it enables long-term storage of genetically valuable gametes. However, knowledge of the diagnostic criteria for assessing sperm quality and the genetic consequences of using cryopreserved sperm in amphibians is limited. This gap has raised concerns in captive breeding and reintroduction programs regarding the potential impact that cryopreserved sperm and the derived embryos may have on a population. The thesis aims to investigate how sperm cryopreservation affects the genetic material in both sperm and resulting embryos, with implications for in situ and ex situ amphibian populations. …

Understanding Pollinator-Forb Interactions And Evaluating Trapping Methods For Pollinator Monitoring In Lawns Of The Southeastern United States, Sara Rose Kirby

Theses and Dissertations

Urban turfgrass landscapes are widespread across the southeastern United States but offer limited ecological value under conventional management. This thesis evaluates the pollinator support potential of ten forb species commonly found in low-maintenance lawns and assesses the effectiveness of two insect sampling methods, sweep netting and pan trapping. Over two years of field surveys, native forbs such as Symphyotrichum divaricatum and Claytonia virginica supported significantly greater insect abundance and diversity than introduced species. Network and modularity analyses revealed that native plants facilitated more specialized pollinator interactions, including those involving oligolectic bee species. In a complementary study, pan traps effectively sampled …

Sweetpotato Insect Pests: Virus Vector Management And Rearing, Rachel Emily Morrison

Theses and Dissertations

Sweetpotatoes (Ipomoea batatas) are vegetatively propagated through slips, making them prone to viral buildup. Aphids are insect pests known for transmitting viruses to crops, which reduces yield. This study examined different options for aphid management to reduce the spread of potyviruses: crop borders, crop oils, and insecticides. However, the results of this research showed that treatments were not successful at reducing the spread of the four potyviruses tested. An additional objective was to establish a colony of banded cucumber beetles (Diabrotica balteata) in the laboratory. These beetles are a leaf-feeding and root-feeding pest of sweet potatoes. Two colonies of banded …