Life Sciences Commons^™

Impact Of A Novel Marine Algae Supplement On Inflammatory And Immune Response After High-Intensity Exercise, Caroline Sara Vincenty

Theses and Dissertations

INTRODUCTION: High-intensity exercise (HIE) can damage the musculotendon complex and impact the immune response, which may result in post-exercise inflammation and reductions in strength and performance. Sufficient rest and recovery will improve muscular resilience against future damaging bouts, however HIE with minimal durations of rest are common in athletic competitions that facilitate persistent inflammation and immune dysregulation. Marine algae-derived fucoidans (MA) have previously shown promise to mitigate pro-inflammatory and immune dysregulation responses, however it has not been investigated in the context of HIE. The purpose of this research is to investigate the safety and efficacy of MA on markers of …

Mechanisms Of Connectivity In Neuronal Development, Janay Vacharasin

Theses and Dissertations

The development of neuronal connectivity requires the careful orchestration of multiple cellular processes including the control of neuronal arborization and synapse formation or function. Defects in these processes during neuronal development will give rise to disorders of neuronal connectivity like autism. In the present work, we examine the genetic and epigenetic mechanisms that build the neuronal wiring of the developing brain. We use human stem cell-derived neurons to study neuronal arborization and function focusing on genetic and environmental risk factors of neurodevelopmental disorders that include autism and intellectual disability. Specifically, we focused on the contribution of the endomembrane, chromatin, and …

Targeting Ribosome Biogenesis For Pancreatic Cancer Treatment, Carlos Perez Iii

Theses and Dissertations

Pancreatic cancer (PanCa) is one of the leading causes of cancer-related death in the United States. Currently, PanCa is one of the recalcitrant cancers that has very limited therapeutic options available for its treatment. The current standard care of PanCa is gemcitabine (GEM) alone or in combination with FOLFIRINOX, nab-paclitaxel, erlotinib, or 5-FU PanCa, which often show poor response. Therefore, new treatment strategies are required for the prevention and treatment of cancer. Ribosome biogenesis process is dysregulated in most of the cancer types of results in production of more ribosomes and synthesis of oncoproteins which lead to the induction, progression, …

Characterization Of The Responses To Chronic Stress In Caenorhabditis Elegans, Amy Laura Knight

Theses and Dissertations

Exposure to chronic temperature stress influences organismal phenotypes that are important for human health, agriculture, and ecology. In this thesis, the model organism Caenorhabditis elegans was used to study the effects of temperature stress on reproduction and lifespan. It was found that worms demonstrated a rapid shut down in egg-laying between 18-24 hours of exposure to 28°C. Despite this reproductive defect, the overall lifespan of worms was unaffected. At the molecular level, heat shock factor 1 (HSF-1), a regulator of the protective molecular pathway known as the heat shock response (HSR), was identified as important for progeny production during heat …

Water-Soluble Porphyrin Complexes For The Biological Inactivation Of Fentanyl, Anneli Nina

Theses and Dissertations

This work aimed to investigate the degradation of fentanyl in aqueous solutions using unsupported water-soluble cobalt(III) porphyrins (55 and 56), AuNP supported water-soluble cobalt(III) porphyrins (67 and 68), and liposome supported water-soluble cobalt(III) porphyrins (69 and 70). Complexes 55, 56, 68, and 69 have been shown to remove fentanyl from aqueous solutions at 37 ºC. The degradation of fentanyl, and the identification of breakdown products were analyzed with the use of time-of-flight mass spectrometry (TOFMS). Complex 55 has been shown to completely degrade fentanyl at 37 ºC in as little as two hours with a 400 mol% catalyst loading. Complex …

Cucurbitacin B: A Potential Natural Agent For Targeting Tumor Immune Cell Population, Emmanuel Anning

Theses and Dissertations

Immune checkpoint blockade (PD1, PDL-1 and CTLA-4) immunotherapies have emerged as the breakthrough in cancer treatment. However, some malignancies show marginal response. One factor that influences the efficacy of immunotherapy is the development of immunosuppressive tumor microenvironment (TME), caused by infiltration of myeloid derived suppressor cells (MDSCs) and tumor associated macrophages (TAM) into the tumor, facilitating metastatic tumor growth and immunotherapy resistance. We have identified Cucurbitacin B (Cuc. B), a potent small molecule that targets TAM and MDSCs and inhibits Stat3, CSF-1R, and PI3Kγ signaling axis at lower doses compared to the pharmacological inhibitor of PI3Kγ (IPI-549). IL-4 polarization of …

Screening For Potential Therapeutic Targets Of Yb-1 Protein Using A Bioinformatics Approach, Omar Muneer Karkoutly

Theses and Dissertations

Treatment options for cancer are becoming much more limited due to the robust characteristics of cancer that allows them to rapidly develop drug resistance. This may be a result of cancer cells’ ability to switch between differentiated and undifferentiated states (plasticity). Diagnostic measurement and detection of cancer and its progression is essential for developing successful treatments. Specific cancer targets whose expressions are highly associated with increased incidence, risk, and spread of cancer therefore become perfect targets for therapeutic intervention. One such novel target that is still being studied is the Y-box binding protein 1 (YB-1), which …

Development Of Enhanced Food Packaging Systems Based On Blends Of Bio-Based And Biodegradable Polymers Exhibiting Antimicrobial Properties, Aishat Agboluaje

Theses and Dissertations

Food packaging is one of the important aspects of food processing, which contributes greatly to decreasing post-harvest food losses. While ensuring that foods are adequately packaged, it is also important to consider the impact of these food packaging materials on the environment. Accordingly, this research work was aimed at investigating the potential of bio-based and biodegradable blends of polyvinyl alcohol (PVA) and chitosan loaded with antimicrobial agents such as turmeric, clove, and cinnamon for use in food packaging applications. The methodology involved creating PVA/chitosan films with varying compositions via solvent casting method and selecting the best composition exhibiting the highest …

Biomimetic Strategies To Control Therapeutic Release From Novel Dna Nanoparticles, Robert J. Mosley

Theses and Dissertations

The inherent chemical, mechanical, and structural properties of nucleic acids make them ideal candidates for the formulation of tunable, personalized drug nanocarriers. However, none so far have exploited these properties for the controlled release of therapeutic drugs. In this dissertation, a biomimetic approach to controlling drug release is exhibited by specifically manipulating the architecture of novel, DNA nanoparticles to take advantage of drug binding mechanisms of action. Rationally designed DNA strands were immobilized on gold surfaces via a terminal thiol modification. Immobilized monomers can be manipulated to form distinct monolayer architectures including flat, folded, coiled, or stretched structures. Increasing the …

International Water Quality: Global Patterns Of Water Pollutants And Pathogens, Leslie Lange

Theses and Dissertations

Water quality is an essential component of vibrant societies and ecosystems. For decades, researchers, managers, and policymakers around the world have struggled to accelerate societal progress while preserving and enhancing water quality and human health. This thesis consists of two studies that I hope will contribute to better understanding, policy, and management. In the first study, I evaluated spatial and temporal patterns in global water quality and their relationship to gross domestic product (GDP) per capita, as a metric of socioeconomic development status. Using global water quality datasets containing over 2.7 million observations, I tested the Environmental Kuznet Curve (EKC) …

Mirnas Levels In A Streptozocin Model Of Alzheimer’S Disease, Nada Moustafa

Theses and Dissertations

Dementia entails a progressive decrease in cognitive functions, with 50%-75% of cases attributed to Alzheimer’s disease (AD); an aging-associated condition characterized by the build-up of tangled phosphorylated Tau (p-Tau) protein and beta-amyloid (Aβ) depositions. Sporadic AD (sAD) is multifactorial in nature, resulting from a combination of environmental and genetic predisposing factors. Type 2 diabetes mellitus (T2DM) is a leading risk factor for dementia, and deregulation of brain glucose metabolism is associated with early cognitive affection in sAD. Thus, the diabetogenic agent Streptozotocin (STZ) is used to experimentally create an AD model in animals (STZ-induced sAD), in which abnormalities in cerebral …

Amino Acids As Alternatives To Emulsifying Salts In Processed Cheese Analogues, Jeremy Thomas Pack

Theses and Dissertations

Background: Manufacturers have used emulsifying salts universally in the processed cheese industry since James L. Kraft patented the first processed cheese in 1916. My objective was to find alternative ingredients to replace emulsifying salts in processed cheese formulations; the product would follow "clean-labeling" trends and lower the final formulation's sodium content. Materials and Methods: My experiments followed conventional processed cheese formulations to create experimental batches, which were compared to positive and negative controls for significance. Textural, rheological, and melting tests evaluated objective cheese parameters. We observed subjective sensory properties through qualitative descriptive analysis, consumer acceptance panels, and focus groups. Results: …

Isothermal Inactivation Studies Of Listeria Monocytogenes, Salmonella, And Enterococcus Faecium Nrrl B-2354 In Almond, Peanut, And Sunflower Butters, Ruo Fen Liao

Theses and Dissertations

Vegetative, non-sporeforming foodborne pathogens show notable survival and uncanny thermotolerance in low water activity (aw) foods. Controlled studies on Listeria monocytogenes, Salmonella spp., and Enterococcus faecium NRRL B-2354 (a Salmonella surrogate) in a variety of food matrices support thermal process validation studies required to achieve global food safety objectives. In this study, we determined and compared thermal inactivation rates using independent six-strain cocktails of pathogens in three plant-based butters. Direct determinations of decimal reduction times (D-values) for L. monocytogenes, Salmonella, and E. faecium, in corresponding butters were inoculated using peanut oil, almond oil, or sunflower oil. Thermal Death Time (TDT) …

Climate Change And The Global Nutrient Overload: The Microbial Response Of Extreme Waterbodies To Environmental Change, Samuel P. Bratsman

Theses and Dissertations

One of the defining characteristics of our current epoch—the Anthropocene—is modification of nutrient cycles. At regional to global scales, humans have fundamentally reshaped the availability of carbon, nitrogen, and phosphorus. These changes are particularly apparent in freshwater ecosystems, which receive surface and groundwater inputs of nutrients from agriculture, fossil fuel use, and wastewater. In this thesis, I investigated how the addition of nutrients affects microbial community and biogeochemistry in two extreme environments: the hypereutrophic shallow Utah Lake and nutrient-limited Arctic permafrost streams. In my first chapter, I used bioassay and dilution bioassay experiments to identify what factors control harmful algal …

Relationships Among Aa-Genome Chenopodium Diploids And A Whole-Genome Assembly Of The North American Species, C. Watsonii, Lauren Amillicent Young

Theses and Dissertations

Chenopodium quinoa Willd., an ancient Andean pseudocereal almost exclusively consumed in South America, jumped onto the global stage when Western cultures noted quinoa's advantageous nutritional profile. Quinoa seed's high protein content, nutritionally balanced amino acid profile, low glycemic index, and high fiber, vitamin, and mineral content, make it a highly sought-after 'superfood'. Pitseed goosefoot (C. berlandieri Moq.), a closely related North American species sharing quinoa's genome composition (AABB), grows across the North American continent, inhabiting diverse environments including the saline coastal soils of the Gulf of Texas and the drought-prone regions of the Southwest. Quinoa and pitseed goosefoot, along with …

The Default Network And Autism Spectrum Disorder: Characterizing Sub-Networks And Behavioral Correlates, Alyssa K. Kozlowski

Theses and Dissertations

The default network (DN), and specifically its sub-networks default network A (DN A) and default network B (DN B), has been strongly implicated in social cognition. This study examined its role in predicting social behavior, and also differences that may exist across diagnostic groups that may explain discrepancies in social cognition and behavior. One of the popular methods of study is functional connectivity, or analyzing correlated activity in the brain. Autism Spectrum Disorder (ASD) is a disorder characterized by social impairment and abnormal social behavior. To date, much of the functional connectivity research in ASD has focused on global connectivity, …

The Power-Duration Relationship Is Just As Reproducible In Females As Males, Despite The Presence Of The Menstrual Cycle, Jessica Joy Linde

Theses and Dissertations

PURPOSE: To investigate the effect of the menstrual cycle (MC) on exercise performance across the power-duration relationship (PDR). We hypothesized females would exhibit greater variability in the PDR across the MC than males across a similar timespan, with critical power (PCRIT) and Work prime (W') being lower during the early follicular phase than the late follicular and mid-luteal phases. METHODS: Eumenorrheic, endurance-trained females (n = 10, age = 24.1 ± 5.59) performed multiple constant-load-to-task-failure and maximum-power tests at three time points across the MC (early follicular, late follicular, midluteal phases). Endurance-trained males (n = 10 age = 29.5 ± 9.18) …

Investigation Into The Genetic Basis Of Capsaicin Production In Peppers Using Next Generation Rna Sequencing And Synthetic Biology Approaches, Ryan Patrick Calhoun

Theses and Dissertations

Capsaicin, a molecule synthesized by plants in the Capsicum genus, is popular for its ability to produce a sensation of burning in any tissue it encounters. The synthesis of capsaicin molecules is achieved through the capsaicin biosynthesis pathway. In this dual study, our goal was to insert two crucial genes, pun1 and pAMT, into a strain of Saccharomyces cerevisiae to allow capsaicin synthesis and perform Illumina RNA sequencing on seven pepper species of increasing pungency to identify other key or novel genes needed or related to capsaicin synthesis. We implemented a golden gate cloning strategy to insert our genes of …

Improving Nutrition And Food Safety Knowledge Of Small-Scale Fish Processors In Delta State, Nigeria, Grace Adeola Adegoye

Theses and Dissertations

Introduction: Fish is an affordable animal source food that provides nutrition and serves as a source of income for many people especially women in Nigeria. Smoking and sun drying are the processing methods in practice that expose the fish products to possible contaminants which may consequentially negate their nutritive value.

Aim: To improve the knowledge of fish processors on nutrition and safe fish handling.

Methodology: A 3-day participatory training was organized to train 122 fish processors, 95 women, and 25 men. The training was conducted in the three senatorial districts in Delta State, Nigeria. Knowledge was assessed using the pre …

Evaluating Soil Health Changes Following Cover Crop And No-Till Integration Into A Soybean (Glycine Max) Cropping System In The Mississippi Alluvial Valley, Alexandra Gwin Firth

Theses and Dissertations

The transition of natural landscapes to intensive agricultural uses has resulted in severe loss of soil organic carbon (SOC), increased CO₂ emissions, river depletion, and groundwater overdraft. Despite negative documented effects of agricultural land use (i.e., soil erosion, nutrient runoff) on critical natural resources (i.e., water, soil), food production must increase to meet the demands of a rising human population. Given the environmental and agricultural productivity concerns of intensely managed soils, it is critical to implement conservation practices that mitigate the negative effects of crop production and enhance environmental integrity. In the Mississippi Alluvial Valley (MAV) region of Mississippi, USA, …

Cover Crops And Irrigation Impacts On Corn And Soybean Production In The Mid-Southern Usa, Dillon Aaron Russell

Theses and Dissertations

Reducing groundwater withdrawals from the Mississippi River Valley Alluvial Aquifer is imperative to sustain future irrigated cropping systems in the mid-southern USA. This research was conducted to determine the impacts of cover crops and irrigation sensor thresholds on corn (Zea mays L.) and soybean (Glycine max L.) production, water productivity, irrigation water use efficiency, and soil physical properties in the Mississippi Delta. The cover crop treatments included cereal rye (Secale cereale L.), hairy vetch (Vicia villosa R.), wheat (Triticum aestivum L.)-radish (Raphanus sativus L.)-turnip (Brassica rapa L.) mix, and no cover crop. The irrigation thresholds included -40 kPa, -90 kPa, …

Determination Of Flexural Strength Of Structural Red And White Oak And Hardwood Composite Lumber, Alan Sherrington

Theses and Dissertations

In this research, flexural properties of mill-run, in-grade white oak and red oak lumber from a single mill and commercially available laminated hardwood composite were evaluated. Structurally graded green (wet) freshly sawn red oak and white oak 5 by 10-cm (2 by 4-in.) nominal lumber as well as glue-laminated hardwood composite billets were tested in bending and their modulus of rupture (MOR) and modulus of elasticity (MOE) properties were developed. It is well documented that MOR and MOE are two major indicators to evaluate flexural strength of wood lumbers. From these data, summary statistics, design values, and mean separations were …

Food Insecurity And Depression Among Us Adults: The National Health And Nutrition Examination Survey, Nicole Reeder

Theses and Dissertations

Food insecurity has long been a global challenge associated with negative health outcomes. The spectrum of food insecurity corresponds to a spectrum of negative health outcomes that range from kwashiorkor and marasmus to obesity and chronic disease. The burden of food insecurity is a stressor that additionally negatively effects mental health. In this study, the relationship between food insecurity and mental health was examined using data from the National Health and Nutrition Examination Survey (NHANES). Food insecurity was assessed with the 18-item United States Food Security Survey Module, and participants were classified into one of four categories based on their …

Nutrients Associated With Stunting Among Children In Sub-Saharan Africa: A Systematic Review, Abby Mae Reynolds

Theses and Dissertations

The purpose of this study was to identify specific nutrients or nutritional biomarkers of dietary intake that are associated with stunting among children ages 2 and older in sub-Saharan Africa. This is a systematic review conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Twelve studies from sub-Saharan Africa met the eligibility criteria, including 11 observational studies and one randomized control trial. Nine of the 12 studies assessed dietary intake of macronutrients. Four studies found a significant association between proteins and stunting. Four found that children with stunting had lower dietary fat intakes or lower …

The Guatemala Rabbit Project: A Household Agricultural Intervention Program Evaluation Using The Re-Aim Framework, Jessica Martin Graves

Theses and Dissertations

Amongst the breathtaking and mountainous landscapes of the Western Highlands is a highly indigenous Mayan population stricken with challenges of food insecurity and extreme poverty. Despite efforts by governmental and non-governmental organizations alike to combat these challenges, the results of chronic malnutrition have led to some of the highest child stunting rates in all of Guatemala, particularly in the department of Totonicapán, where this study took place. To date, information is limited regarding agricultural programs that utilize the rabbit as a model to reduce food insecurity in Guatemala. Thus, a novel agricultural education program, Guatemala Rabbit Project (GRP), was developed …

Diversity-Productivity Relationships In Forests Of The Southeastern United States: Leveraging National Inventory Data And Tree Functional Traits, Elizabeth Baach

Theses and Dissertations

Numerous studies have evaluated the relationship between biodiversity and productivity, with general trends suggesting a positive relationship. While most studies only use species richness, this study also analyzed how productivity changes with functional diversity and Shannon’s diversity index. Functional diversity gives important context to the examination of biodiversity-productivity relationships due to the direct link between organisms’ functional traits and their role in a given ecosystem. This study used data from the USDA Forest Service Forest Inventory and Analysis (FIA) database collected in Alabama, Louisiana, and Mississippi to estimate plot-level productivity and diversity. Structural equation modeling was used to determine the …

Procedures For Genetic Modification Of Miscanthus X Giganteus, A Biomass Crop, Anjali Arpan

Theses and Dissertations

Development of genetic improvement procedures for the bioenergy crop, Miscanthus × giganteus (M×g; triploid), are desired for trait improvement. Agrobacterium tumefaciens has been used in other Miscanthus spp. for genetic improvement, but not in M×g, nor have more than a few genes been introduced at a given time. Transformation procedures for M×g were developed; studies included use of A.tumefaciens (strain LBA4404) and A. rhizogenes (strain A4) for introduction of seven genes isolated (by other researchers) from Sorghum bicolor involved in sorgoleone biosynthesis (an allelopathic compound), and selectable marker gene neomycin phosphotransferase II. Procedural development included generation of …

Growing Tea In Mississippi, Qianwen Zhang

Theses and Dissertations

Tea is the most popular beverage worldwide, second only to water. The United States is the third largest importer of tea in the world, importing a total of about 260 million pounds tea annually. With increased demand for locally sourced food products, questions are raised regarding whether domestic production of tea is feasible. Compared to major tea producing countries with centuries of growing history, tea production in the U.S. is limited and requires research support in many aspects of tea production. The objectives of this study were to investigate plant growth, leaf morphology, and seasonal chemical compositions in tea leaves …

Effects Of Pruning Timing, Leaf Removal, And Shoot Thinning On 'Midsouth' Winegrape Quality In South Mississippi, Haley Nicole Williams

Theses and Dissertations

‘MidSouth’, a relatively low maintenance interspecific hybrid bunch grape currently grown in South Mississippi, has low sugar and high acid levels for red wine use. Two studies, conducted at the Mississippi State University McNeill Research Unit in 2020 and 2021, determined the effects of pruning timing, leaf removal, and shoot thinning on ‘MidSouth’ development and fruit and wine quality. Treatments in the first study included early versus normal pruning timing, both with and without leaf removal, and treatments in the second study included leaf removal, shoot thinning, and control vines. Cluster temperatures, leaf chlorophyll, berries per cluster, berry and cluster …

Novel Strategies In Near Infrared Spectroscopy (Nirs) And Multivariate Analysis (Mva) For Detecting And Profiling Pathogens And Diseases Of Agricultural Importance., Johjan Mariana Santos Rivera

Theses and Dissertations

The time required for the identification of pathogens is an important determinant of infection-related mortality rates and disease spread for species of relevance in agriculture. Conventional identification methods require a processing time of at least one to twenty days. Therefore, inaccurate empirical treatments are often provided while awaiting further identification, such that most cases progress with further aggravation of symptoms, contamination of other animals or plants, generating economic loss from decreased yield, and increased mitigation costs. Thus, there is a need for innovative, non-destructive, and rapid analytical techniques that provide reagent-free, portable, reliable, and holistic approaches to detect diseases in …