Life Sciences Commons^™

Cyclomorphosis Of Daphnia Lumholtzi In Response To Spatial Heterogeneity In Lake Taylorville, Karen K. Schnake

Masters Theses

Reservoir zonation occurs longitudinally as water enters from a stream into an impoundment. Inflow areas (or riverine zones) are typified by high flow rate and shallow depth whereas areas near the dam (lacustrine zones) characteristically have decreased flow and greater depth. In a typical reservoir, abiotic and biotic variables change somewhat predictably along a continuum from the riverine, through a transitional and into a lacustrine zone which can have a significant affect on the biota. Daphnia lumholtzi, a cladoceran which exhibits extreme cyclomorphosis, is an exotic species introduced to North America within the past twenty-five years. I examined the morphology …

Effects Of E2 On Apoe Secretion And Neurite Outgrowth In Cultured Adult Mouse Cortical Neurons, Fei Shen

Masters Theses

Estrogen replacement therapy appears to delay the onset of Alzheimer's disease (AD). The mechanism whereby estrogen prevents the pathogenesis of AD is unknown. In the present study, I examined the effects of 17-β-estradiol (E2) on neurite outgrowth from adult mice cortical neurons (AMC) in culture. I found that E2 increases apoE secretion and neurite outgrowth in AMC neurons from wild type mice in a dose dependent fashion. The neurite outgrowth promoting effect of E2 was not observed in AMC neurons derived from age-, sex-, and stain-matched apoE deficient/apoE gene knockout (apoE KO) mice. Furthermore, E2 has isoform specific effects on …

Gas Exchange And Yields Of Bt Resistant Maize (Zea Mays L.) With European Corn Borer (Ostrinia Nubilalis, Hubner) Infestation, Shad Mallady

Masters Theses

Seed companies have transgenic maize (Zea mays L.) hybrids resistant to European corn borer (ECB) [Ostrinia nubilalis (Hübner)]. However, the impact of this Bt (Bacillus thuringiensis) gene on other plant processes is not understood. In 1997 and 1999, a study at Central Golden Harvest Research in Clinton, IL focused on how the Bt gene affects gas exchange and yields of maize with and without ECB infestation. Bt and non-Bt isogenic pairs were planted with or without nets and/or insecticides to eliminate natural infestation of ECB, and with or without manual infestation of ECB. Photosynthesis and transpiration …

Modulation Of Apolipoprotein E Expression And Glial Activation In Mouse Olfactory Bulb During Estrous Cycle: An Immunohistochemical Study, Matthew Kircher

Masters Theses

Estrogen (E2) plasma levels fluctuate as a function of estrous cycling in the mouse. Apolipoprotein E (apoE) mRNA has been shown to increase in both hippocampal astrocytes and microglia during proestrus when plasma estrogen levels are high. The current study was designed to evaluate apoE localization and glial activation in mouse olfactory bulb (OB) as a function of the estrous cycle. In the rat OB, estrogen receptor β (ERβ) is present in the glomerular and external plexiform layers, but not the internal plexiform and granule cell layers. Normal female mice 2-3 months old at the start of the study were …

Aging Coyotes Using Dental Characteristics, Michelle Maher

Masters Theses

The accepted methods of age determination in the coyote (Canis latrans) are either highly subjective and unquantifiable or expensive and require the extraction of the canine tooth. Since neither of these methods are ideal, their limitations have impeded research on this species. Therefore, it was my objective to (1) develop and test the accuracy and precision of a descriptive key based on tooth wear patterns on the lower canine tooth, (2) develop and test the reliability of multiple regression models for aging coyotes using measurements from extracted teeth, and (3) suggest criteria for improving the consistency of results …

Reproductive Biology Of Stylisma Pickeringii (Convolvulaceae), An Endangered Plant Of Illinois Sand Prairies, Brent L. Todd

Masters Theses

The sand prairie is a unique ecosystem in the state of Illinois, being the habitat for many interesting plants and animals not found elsewhere in the state. One such plant is the state-endangered Stylisma pickeringii (Torr. ex M.A. Curtis) Gray var. pattersoni (Fern. & Schub.) Myint (Convolvulaceae), also known as Patterson's bindweed or Patterson's dawn flower. It currently is found in only three Illinois counties—Cass, Henderson, and Mason. Presently, two of the three populations of S. pickeringii are located on private lands. Very little information is known about the reproductive biology of S. pickeringii var. pattersoni. Thus, the purpose of …

Lateral Migration Of A Solid Particle In Shear Flow, Wutthapong Jiraporncharoen

Masters Theses

Lateral migration of solid particles in shear flow is observed in various industrial applications. Suspensions in a blade coater, a device used to apply coating pigment to a base paper to improve paper properties, is an example. Particle hydrodynamics will affect the quality of coated paper and runnability of the coater especially when the speed of the coating machine is very high or the pigment concentration is increased.

Many factors will affect the particle migration. The wall repulsion or lubrication effect forces the particle away from a wall to the channel center. The inertial effects are increased by increasing the …

Enzymatic Deinking Of Flexographic Water-Based Inks, Somporn Chaiarrekij

Masters Theses

Introduction

In recent times, environmental concerns have led to the development of water-based flexographic and rotogravure inks. One of the major concerns for water-based inks is their deinkability. Wash deinking of flexographic water-based ink is much more effective than flotation deinking; however, it has two major disadvantages. It consumes large amounts of water and gives a lower yield than flotation due to the loss of fines and fillers in the effluent (1).

Development And Characterization Of Monoclonal Antibodies Directed Against Yaba-Like Disease Virus, Scott Haller

Masters Theses

The hybridoma technique has been used for the production of monoclonal antibodies (mAb) directed against yaba-like disease virus (YLDV). Spleen cells, obtained from female BALB/c mice immunized with YLDV, were fused with P3x63.Ag8.653 myeloma cells and hybrids were selected. Hybridoma clones were screened for production of anti-YLDV antibodies by Indirect Immunofluorescence (IF) microscopy. Positive hybridoma clones were recloned via limiting dilution and characterized. A single clone (H12) has been selected that specifically reacted with YLDV infected cells and not uninfected cells. Immunodiffusion technique determined H12 monoclonal antibody as an IgG1 isoptype immunoglobulin. H12 antibodies recognized a late YLDV protein, and …

Influence Of Starch Molecular Weight And Sodium Alginate On Silicone Holdout For Release Papers, Peter Suchy

Masters Theses

This research was focused on determining the influence of applying one type of potato starch of various molecular weights on silicone holdout. Also, combinations of potato starch with a rheology modifier, sodium alginate, of low, medium, and high molecular weight were studied. The base paper employed was a commercially available paper generally used to manufacture release papers.

Sizing with low molecular weight potato starch is more effective for silicone holdout than sizing with high molecular weight potato starch. This study showed that the paper sized with low molecular weight potato starch has a smoother surface, lower permeability, and a smaller …

Hot Melt Inks For Rotogravure - Formulation, Printability And Rheology, James Atkinson

Masters Theses

A new generation of phase change, or hot melt ink will be formulated. This ink was solid at ambient temperature and liquid at the moment of printing. Inks will be formulated on the basis of polyethylene and Ethyl Vinylacetate chemistry. Inks were printed using a Meyer rod for screening printability study. Selection of the best formulations will be done on the basis of reflective density, specular gloss, mottle, and rub resistance. Screened inks having best properties were printed on the Moser laboratory sheetfed proofing press. More detailed printability analysis was done after Moser printing, including dot structure analysis, tone gradation, …

Assessing Silicone Holdout For Release Papers, Rudolf Haraga

Masters Theses

Silicone coating is widely used on papers that require low surface tension. Such papers are called release papers and are used in label, food, and packaging applications. Before silicone coating is applied, the base paper undergoes sizing procedures to smooth the surface and to cover voids present in the surface of the paper substrate. After the base paper is properly sized, it is silicone coated. This makes the surface of the base paper have a low surface energy. Therefore, when a second adhesive paper is applied to the base paper, the second adhesive paper can be easily recovered from the …

Renesting - Female's Success After Nesting Failure And The Renesting Of The Dickcissel (Spiza Americana) At Prairie Ridge State Natural Area, Kevin L. Wentworth

Masters Theses

Renesting - Female's success after nesting failure. Many birds are unsuccessful in their first nesting attempt, so renesting is often a very important way for birds to increase their lifetime fitness and for populations to maintain stable numbers. I examined the importance of renesting and the factors that encourage or discourage a female from renesting and also the factors that affect the success of these renests. I also looked for patterns in renesting behavior (e.g., renesting interval, renesting distance, percentage of females that attempt renests, and the success of renests) of birds by comparing different studies. I used a total …

The Effects Of Social Physique Anxiety Levels, Body Mass Index, And Program Type On Exercise Adherence And Reasons For Exercise, Colleen N. Vandever

Masters Theses

The purpose of this study was to compare adult females across various age groups, body mass index, and exercise facilities on their levels of social physique anxiety, reasons for exercise, and adherence rates over an 8-week period. Female members of the Eastern Illinois University Adult Fitness Program (n = 14), Curves for Women (n = 19), and the Sarah Bush Lincoln Health Center Employee Wellness Program (n = 14) took part in the study. Two age group categories were made: 30-50 (n = 21) and 51+ (n = 26) in order to compare female age groups. Specifically, the purposes of …

Managing Wetland Vegetation For Marsh Birds And Waterfowl At Carlyle Lake Wildlife Management Area, Il, Matthew W. Bowyer

Masters Theses

The high cost of restoring and managing wetlands warrants careful assessment of their management potential. We designed this study to provide basic information on how seed banks and hydroperiod influence the development of habitat for migratory birds in restored wetlands at Carlyle Lake Wildlife Management Area (CLWMA). The objectives were to: (1) survey the composition of the seed banks; (2) investigate the effects of early- vs. late-drawdowns on the germination and growth of these plants; and (3) monitor the use of these communities by wetland birds during migration and the breeding season. The study was conducted on a marsh complex …

Olfactory Behavior In Apoe Knockout Mice, John P. Yost

Masters Theses

Apolipoprotein E (apoE), a lipid transporting protein, has been shown to play a vital role in nerve repair and remodeling. Since the olfactory system is in a continuous state of remodeling, the present study tested the hypothesis that apoE is required for normal functioning of the olfactory system. Olfactory function of the wild type (WT) and apoE deficient (apoE KO) mice was assessed by using three standard olfactory tests: 1) the buried food pellet (BFP) test; 2) the odor choice (OC) test; and 3) the odor cued taste avoidance (OCTA) test. The visible food pellet test (VFP) was not used …

Isolation Effects Of Aquatic Habitat Fragmentation On Greenside (Etheostoma Blennioides) And Fantail (Etheostoma Flabellare) Darters, Rita M. Klein

Masters Theses

Aquatic habitat fragmentation is defined as the lack of connectivity between upstream and downstream populations and can occur along two gradients, the longitudinal gradient and the lateral gradient. The longitudinal gradient focuses on objects within the stream and is concerned with the disruption of stream flow, which is normally brought about by the presence of dams and bridges. Fragmentation along the lateral gradient, on the other hand, examines factors outside of the stream and is related to any loss or modification of the surrounding riparian zone.

In Illinois within the last 100 years, stream habitats have become increasingly fragmented by …

The Effects Of Strength Training On The Bone Mineral Density Of Aerobically Trained Perimenopausal Women, Jill Marie Kowalski

Masters Theses

The purpose of this study was to determine if a strength training program has an effect on the bone mineral density of aerobically trained perimenopausal women. The study also hoped to determine if high intensity strength training with low repetitions will result in a greater increase in bone density as compared with low intensity strength training with high repetitions. The duration of the study was six months.

The participants were twenty-five aerobically trained perimenopausal female volunteers. They were randomly selected to be in one of three groups. Group 1 was the aerobic control where the participants continued with their aerobic …

Identification And Characterization Of Zinc-Responsive Genes In Pseudomonas Fluorescens, Thomas L. Wilson

Masters Theses

Genes responsive to elevated concentrations of zinc were identified via transposon mutagenesis in the common soil bacterium, Pseudomonas fluorescens ATCC13525. Some of these genes were essential to maintain metal homeostasis in the bacterial cell. DNA sequences of the transposon-tagged genes were determined by DNA sequencing of arbitrary PCR products. DNA sequence analysis indicated that one gene was similar to P-type ATPases responsible for transporting metal ions out of the cell. The corresponding mutant was sensitive to zinc, cadmium, and lead indicating that this gene may be responsible for defending Pseudomonas fluorescens against these metal ions. Four zinc-induced genes shared similarity …

Cadmium Induced Gene Expression And Signal Transduction Pathway, Daisuke Maki

Masters Theses

Cells respond to cadmium (Cd), a common environmental contaminate, by potentially decreasing reduced intracellular glutathione, as well as, increasing the expression of several antioxidant genes. One of these antioxidant genes, glucose-6- phosphate dehydrogenase (G6PDH), is the rate limiting enzyme of the pentose phosphate pathway. This pathway produces NADPH which is used for maintenance of reduced glutathione so, it is hypothesized that Cd should increase G6PDH expression. In this study, we demonstrate that Cd increases the level of G6PDH mRNA in primary rat hepatocytes in culture. In the presence of the antioxidants, N-acetylcysteine (NAC), Trolox, or glutathione monoethyl ester an attenuation …

The Influence Of Base Sheet Sizing On The Print Quality Of Matte Coated Ink Jet Papers, Saurabh K. Halwawala

Masters Theses

The most recent developments in ink-jet papers have been related to coating aμd pigment technology. Silica is the preferred pigment for matte coated ink-jet papers. Besides being expensive, the high surface area and porosity of this pigment limits the percentage coating solids to less than 30-35%, which increases production cost due to higher dryer loads. Thus, a need exists to develop a new generation of pigments. The objective of this research was to evaluate the effect of base sheet absorbency on the surface, as well as print properties of matte coated ink-jet papers using a new pigment capable of being …

Effect Of Size Level And Fiber Coarseness On Ink-Jet Printability Of Size Press Coated Paper, Falguni Mehta

Masters Theses

In recent years, matte coated paper has enjoyed wide spread usage for ink-jet applications. The coating performance is strongly influenced by base sheet properties including fiber coarseness and base sheet absorbency. Base sheet absorbency, which is elevated in terms of internal sizing, is a crucial property of ink-jet paper.

The objective of this research was to evaluate the influence of size level and fiber coarseness on ink-jet print qualities. A secondary objective was to examine the size reversion phenomena and to quantify the effect of size level, pigmented coating, and fiber coarseness on size reversion.

The results showed that the …

Role Of Dopamine D4 Receptor In The Genomic And Behavioral Effects Of Psychostimulants: Relevance To Schizophrenia, Bina Garimella

Masters Theses

The role of dopamine D4 receptors in the induction and expression of behavioral sensitization to amphetamine, cocaine and PCP was investigated in this study. Behavioral changes and genomic responses accompanying sensitization were examined using a highly selective D4 receptor antagonist, PNU-101387G. Induction and expression of behavioral sensitization to cocaine was blocked by PNU-101387G. However, the D4 antagonist did not modulate the induction and expression of behavioral sensitization to PCP. Neuroadaptive genomic responses accompanying acute and chronic effects of amphetamine and their modulation by PNU-101387G was examined by looking at alterations in the expression of two immediate-early genes: c-fos and NGFI-A. …

Development Of A Photoinitiator Blend For Uv Cured Inks And Coatings, Madhu Bansal

Masters Theses

The purpose of this study is to develop a new standard blend of photoinitiators, having properties of low odor, high solubility, faster curing, and being efficient for pigmented as well as for clear systems. In the process, we expect to understand the behavior of mixtures of photoinitiators and their resulting UV spectra. An important first step is to prepare different blend with different photoinitiator and characterize them on cure basis. Curing properties were studied by the KMnO₄ yellow stain density test. The absorption range in the UV spectra was measured for each Photoinitiator with methanol and acrylate as background …

The Role Of Nf-Кb Activation In Breast Carcinoma And Glioblastoma Cell Lines, Kelly T. Murphy

Masters Theses

The nuclear factor-КB (NF-КB)/Rel family of inducible dimeric transcription factors regulates numerous genes associated with the immune, mitogenic, and apoptotic responses. Investigations were made into the role of NF-КB in breast carcinoma (MCF-7, T47D, MDA-MB-231) and glioblastoma (U87MG, U373MG, Ul 18MG) cell lines. NF-КB binding to DNA was quantified using both gel mobility shift assays and transient transfections of a vector containing a luciferase gene under the control of 4 tandem copies of the NF-КB binding sequence. To determine the correlation between NF-КB activation, cellular proliferation, and foci formation, the tumor cells were cultured and analyzed in the presence of …

Isolation And Characterization Of A Genetic Locus That Confers Tolerance To Cadmium And Zinc In Pseudomonas Fluorescens, Szi Fei Feng

Masters Theses

This thesis describes the isolation and characterization of a region containing several genes involved in metal tolerance in the common soil bacterium, Pseudomonas fluorescens ATCC 13525. The DNA region was identified in a cadmium and zinc sensitive mutant, 45-B10, which contains a lacZ-based reporter gene transposon Tn5-B20 inserted in the chromosome. The DNA surrounding the transposon was cloned from this mutant strain and DNA sequencing of this region revealed seven genes that share similarity with genes whose products are involved in the cation-proton antiporter efflux system of other Gram-negative bacteria. Complete DNA sequence was obtained from four of these …

An Investigation Into The Influence Of Common Fillers On Color Reversion Of Bctmp Pulp, Joseph N. Mikowski

Masters Theses

Recent advances in the pulping and bleaching of high-yield pulps, particularly bleached thermomechanical pulp, have made these pulps suitable replacements for bleached kraft pulps. Unfortunately, these high-yield pulps are not appropriate for making high-grade papers because of their tendency to yellow. Extensive efforts have been made to reduce the yellowing, but none of the methods that have been investigated have been at once both practical and economical.

This study investigated the ability of common pigments used in the paper industry to reduce the extent of yellowing of an 80/20 blend of bleached chemithermomechanical and bleached softwood kraft pulp. The effectiveness …

Effects Of Elevated Carbon Dioxide And Nitrogen Fixation On A Legume-Based System: A Test With Soybean And The Soybean Looper, Pseudoplusia Includens, Angela M. Spickard

Masters Theses

Wild-type and non-nodulated soybean were grown at 367 ± 10 ppm [ambient] and 756 ± 10 ppm [elevated] CO₂ to determine whether nitrogen fixation could minimize the effects of elevated CO₂ on plant nutritional and defensive chemistry. Relative to non-nodulated soybean, wild-type soybean had higher concentrations of leaf nitrogen and proteinase inhibitors, as well as lower C:N ratios and lower phenolics. Elevated CO₂ resulted in decreased leaf nitrogen and proteinase inhibitor contents, and increased C:N ratios and phenolics in both soybean strains. Constitutive levels of phenolics and proteinase inhibitors occurred as predicted by the Carbon/Nutrient Balance hypothesis …

Borax As Internal Stock Additive In Papermaking, Nejib S. Yousef

Masters Theses

The effect of borax on strength property of paper has been investigated in different ways. The use of borax either improved the physical properties or assisted in the reduction of refining energy to significant level.

The study of borax in the presence of precipitated and ground calcium carbonate was accomplished by using white water recycling. In the absence of retention aids, recycling of white was a necessary condition for the retention of ash content. As the ash content increased the strength property decreased. But the presence of borax slowed down the detrimental effect of fillers.

Sizing development progressively improved especially …

Target-And Stage-Dependent Influences On Axonal Outgrowth Of An Identified Motorneuron In Leech Cns Explants, April Crowley

Masters Theses

The neural circuit that controls rhythmic contraction of the hearts in the medicinal leech, Hirudo medicinalis, provides an amenable model to investigate pathfinding and target selection. The bilaterally paired heart tubes (HT) in leech are eventually innervated by the heart excitor motomeuron (HE) following a period of remodeling that requires the presence of the HT. To begin characterizing possible HT-derived cues, co-cultures of CNS with innervated HT have been used. Previous work demonstrated that young, but not adult, HT was able to support profuse general outgrowth, but the HE extended few projections onto HT of any age. Current studies …