Life Sciences Commons^™

Computational Modeling Of Β5-Β6 Carbapenemase Loop In Wild Type And Selected Mutants Of Oxa-24 Class D Β-Lactamase, James Roland Lafleur

Masters Theses

Bacterial resistance to antibiotic therapies, especially to β-lactams, is an increasing problem. β-lactamases are the main source of clinical resistance to these antibiotics, and the class D β-lactamases are one of the most rapidly expanding classes of these enzymes. The emergence of class D enzymes with the ability to hydrolyze the newest family of β-lactams - the carbapenems - is a serious concern for the healthcare system as carbapenems are last resort antibiotics: ideal for severe infections after other therapies have failed. Class D β-lactamases are very diverse in terms of sequence and substrate profile, and it remains unclear what …

Evolution Of Invasive Species In Response To Management: Potential For Herbicide Resistance In Watermilfoil, Lindsey-Ann Louise Schulte

Masters Theses

Herbicides are commonly used to control invasive plants. While agricultural weed scientists have focused on herbicide resistance, little is known about its importance in nonagricultural settings. Hybrid Eurasian watermilfoil (Myriophyllum spicatum x M. sibiricum) is an invasive aquatic plant that is frequently managed with herbicides throughout the United States. In this thesis, I consider the potential for natural populations of this taxon evolving resistance to 2,4-dichlorophenoxyacetic acid (2,4-D), the most commonly used systemic herbicide. Evolutionary change in a trait between generations as a result of selection requires both genetically based variation of the trait within a generation (heritable …

Photophysiological Studies Of Cyanobacterial Mats In Submerged Sinkholes Of Lake Huron, Michael Snider

Masters Theses

No abstract provided.

Documenting Annual Differences In Vegetation Cover, Height And Diversity Near Barrow, Alaska, Timothy Frederick Botting

Masters Theses

Vegetation in the Arctic has been shown to respond to climate change. Documented changes have the potential to result in numerous ecosystem consequences. Therefore, understanding vegetation change is of great importance. This study documents changes in tundra vegetation with a focus on understanding the influence of annual differences in weather. Vegetation was sampled using a point frame method on 98 1-m² plots in 2010 and 2013 near Barrow, Alaska. A subset of 30 of these plots was also sampled in 2012 and 2014. Plant encounters were identified to species and grouped into one of the following functional groups: bryophytes, …

Assessment Of Northern Bobwhite Survival And Fitness At Felsenthal National Wildlife Refuge, Arkansas, Jacob W. Doggett

Masters Theses

In the West Gulf Coastal Plains (WGCP) northern bobwhite (Colinus virginianus) numbers are declining faster than range-wide averages and such declines have been linked to the consequences of land management within the region. Management for the endangered red-cockaded woodpecker (Picoides borealis) has benefitted northern bobwhite by restoring mature pine-grassland ecosystems in some areas of the region; however, at Felsenthal National Wildlife Refuge, Crossett, Arkansas, USA, the bobwhite population was not increasing despite the availability of seemingly suitable habitat from management for the endangered species. To understand factors that may be affecting bobwhite survival on Felsenthal National …

Allelopathic Effects Of S. Canadensis On The Germination Of Native Prairie Plants, James David Megenhardt

Masters Theses

Solidago canadensis has become a aggressive species of North American prairie forb throughout the world and within restored prairies throughout North America, causing the loss of biodiversity within those ecosystems. While reproductive methods of wind dispersed seeds and clonal expansion through rhizomes has helped S. canadensis colonize and spread through these ecosystems, S. canadensis also releases allelopathic chemicals which may inhibit the germination and growth of competing species. What was unknown is if these same allelopathic chemicals which give S. canadensis an edge within foreign ecosystems might also explain why S. canadensis is so prolific in restored North American …

Daily Activity Patterns In Three Migratory Bird Species At A Stopover Site On The Northern Coast Of The Gulf Of Mexico, Lynn Schofield

Masters Theses

CHAPTER I: A Comparison of Diel Movement Patterns in Three Bird Species at a Stopover Site on the Northern Coast of the Gulf of Mexico

During migration, birds require stopover habitats where they stop en route to rest, refuel, and prepare for the next stage of their migration. For songbirds, many short movements made within a stopover habitat are known to be costlier than a single long migratory flight, as it takes a considerable amount of energy to initiate flight. This indicates that migrating birds should strive to balance the energy expenditure made in movement during stopover and energy gains …

Asian Carp Population Status And Reproductive Potential In Illinois River Tributaries, Clinton Wesley Morgeson

Masters Theses

Large floodplain rivers are among the most complex natural systems characterized by exchange between the river and its floodplain. Information regarding fishes in main channel habitats is abundant, however much less understanding extends into their tributaries. Tributaries of large rivers provide critical habitats for main channel fishes, important for foraging, spawning, and refuge. In addition to native diversity, large rivers are host to multiple exotic species invasions, threatening biodiversity, ecosystem function, and habitat quality. Two notorious invaders are Silver Carp (Hypophthalmichthys molitrix) and Bighead Carp (H. nobilis), collectively Asian Carp, presenting an imminent threat of invasion …

Compatibility Factors Of Fascioloides Magna Miracidia And Four Sympatric Snail Species: Miracidial Behavior And Snail Response, Bryan Rolfsen

Masters Theses

Miracidia exhibit observable host-finding behaviors, and their sympatric snails have attributes that either permit or prohibit infection. This study was designed to assess the factors involved in intermediate host finding and host-parasite compatibility in the deer liver fluke (Fascioloides magna). The main aim was to determine the extent to which the parasite and/or the intermediate host are involved in host-parasite compatibility. A secondary goal was to determine what factors may lead to miracidial transformation. The study used a panel of four sympatric snails (Lymnaea caperata, Lymnaea palustris, Lymnaea exilis, and Physa sp.) that display …

Adaptive Significance Of Aberrant Chickadee Song In Illinois, Evan Andrew Glynn

Masters Theses

I studied the behavioral responses of Black-capped (Poecile atricapilus) and Carolina (P. carolinensis) chickadees to playback of Black-capped, Carolina, and aberrant chickadee songs throughout the state of Illinois. These two species are parapatrically distributed throughout the eastern United States, including Illinois. Some areas exist where the ranges of the two species overlap. Individuals in these contact zones often sing aberrant vocalizations. This study focused on the two parental vocalizations (i.e., Black-capped and Carolina song), and two of the most common aberrant vocalizations (the 'Greenville' and 'Vandalia' aberrant dialects), in an attempt to understand the adaptive significance of these aberrant songs. …

The Role Of Creg1 And Pitx1 As Master Regulators Of Liver Function, Anit Shah

Masters Theses

Development in mammals requires a complex array of positive and negative regulatory signals and responses. The liver is a major organ that has been studied extensively to understand underlying genetic processes responsible for specification, establishment and maintenance of tissue identity. Hepatoma and hepatoma variant cell lines have been used as a model to understand genetic networks responsible for liver function. Whole genome analysis of these cell types has identified candidate genes that might serve as master regulators of liver identity. In this study, the role of two candidate genes - cellular repressor of E1A stimulated gene (CREG1) and paired-liked homeodomain-1 …

The Effects Of Kinesio Tape On Sports Performance: Vertical Jump And Shuttle Run Performance In Women's College Basketball Players, Holly Wohltman

Masters Theses

Introduction: Since Kinesio tape (KT tape) was invented in the mid-1970s, studies have been lacking in producing strong evidence to support the claims of its ability to enhance performance. The purpose of this study was to determine the effects of KT tape at enhancing sports performance, in the areas of vertical jump and shuttle run performance, in Illinois College Women's Basketball Team, participants ranged from 19-22 years of age.

Methods: There were eleven female basketball players ranging from the ages 19-22 years were recruited to participate in this study. All participants performed a vertical jump test and a shuttle run …

D-Galactose Decreases Mitoneet Levels In Liver Cancer Cells: Impact On Cellular Bioenergetics, Sudip Paudel

Masters Theses

Galactose is a simple sugar that at supraphysiological concentrations accelerates aging and age-related complications, which lead to impaired mitochondrial functions. MitoNEET is a small mitochondrial membrane protein with a molecular mass of 12.2 kDa that functions in diabetes, iron metabolism, regulation of oxidative phosphorylation (OXPHOS) and reactive oxygen species (ROS) homeostasis. MitoNEET knockdown increases cellular respiration rates and ROS levels similar to galactose treatment. Pioglitazone, an antidiabetic drug, binds to mitoNEET and ameliorates galactose toxicity. Cellular mitoNEET levels, exposure of cells to galactose medium, and pioglitazone treatment directly influence cellular respiration. To elucidate the role of mitoNEET in galactose induced …

The Effects Of Solutes In The Cryopreservation Of Adherent Neuroblastoma (Neuro-2a) Cells, Trisha L. Bailey

Masters Theses

A simple method to cryopreserve adherent monolayers of neuronal cells is currently not available, but the development of this technique could facilitate numerous applications in the field of biomedical engineering, cell line development, and drug screening. However, complex tissues of some exceptional animals survive freezing in nature. These animals are known to accumulate several small molecular weight solutes prior to freezing. Following a similar strategy, we investigated the effects of osmolytes such as trehalose, proline, and sucrose as additives to the traditional cryoprotectant dimethyl sulfoxide (Me2SO) in modulating the cryopreservation outcome of mouse neuroblastoma (Neuro-2a) cells. Neuro-2a cells adhered to …

Bridging In Vi Tro & In S Il Ico Techniques: Uncovering The Structural Characteristics And Light-Dependent Modifications Of The Light-Response Btb Proteins In Arabidopsis Thaliana, Nicholas Reitz

Masters Theses

The conjugation of the NEDD8 protein has been found to cause a wide range of changes to the overall function of a protein. The addition of NEDD8 to a conserved lysine on Cul proteins has been found to provide Cul with increased flexibility, while the transcription factor protein p53 was prevented from binding to DNA after NEDD8 conjugation. Using Arabidopsis thaliana, we investigated the potential conjugation of NEDD8 to the AtLRB1 and AtLRB2 proteins, which are both members of Cul3-RING E3 Ubiquitin Ligase complexes, using both in vitro and in silico techniques. The AtLRBs are negative regulators of the red …

Ph Dependent Antibiotic Resistance Of An Alkaliphilic, Halotolerant Bacterium Isolated From Soap Lake, Washington, Tiffany Charlynn Edwards

Masters Theses

"Soap Lake, located in Washington State, is a meromictic, soda lake. Many bacterial isolates retrieved from Soap Lake have been noted to possess resistance to multiple antibiotics. A likely explanation for the wide range of antibacterial resistance exhibited by these strains is due to the impact of high alkalinity on the antibiotics themselves and not due to the presence of antibiotic resistance genes. The aim of our study was to determine if select antibiotics are effective against Halomonas eurialkalitoleranis, a bacterium capable of growth over a wide range of neutral to alkaline pH values, to investigate the influence of alkalinity …

Healing Of Bone Defects In A Rodent Calvarial Defect Model Using Strong Porous Bioactive Glass (13-93) Scaffolds, Yinan Lin

Masters Theses

"The main objective of this project was to evaluate the capacity of strong porous silicate (13-93) bioactive glass scaffolds prepared by a robocasting technique to regenerate bone and stimulate angiogenesis in a rat calvarial defect model. The scaffolds were created with the same grid-like microstructure but in a variety of formulations: (i) as-fabricated, (ii) pretreated in an aqueous phosphate solution to convert the glass surface to hydroxyapatite, (iii) loaded with bone morphogenetic protein-2 (BMP2) (1 µg per scaffold), and (iv) doped with copper (0.4-2.0 wt. % CuO). When compared to the as-fabricated scaffolds, the pretreated scaffolds enhanced bone regeneration at …

Effects Of Treated Wastewater Effluent On Microbial Community Structure In A Natural Receiving Aquatic System, Matthew D. Hladilek

Masters Theses

Despite our dependency on treatment facilities to condition wastewater for eventual release to the environment, our knowledge regarding the effects of treated water on the local watershed is extremely limited. Responses of these lotic systems to the treated wastewater effluent have been traditionally investigated by examining the benthic macroinvertebrate assemblages and community structure; however, these studies do not address the microbial diversity of the water systems. In the present study, planktonic and benthic bacterial community structure were examined at fourteen sites (from 60 m upstream to 12,100 m downstream) and at two time points along an aquatic system receiving treated …

Hamstring Training Effects On Strength Gains In Ncaa Division I Varsity Soccer Players, Andrea M. Chilcote

Masters Theses

Those athletes who run at high speeds require eccentric strength when the hamstring muscles are in a lengthened state in order to absorb the increased forces, decreasing injury rate and performance deficits (Schmitt, Tyler & McHugh 2012). The purpose of this study was to determine if the intervention of an eccentric hamstring strengthening program would increase peak torque production in eccentric and concentric hamstring and quadriceps movements in Division I Men and Women Varsity soccer players.

Participants included 8 Division I soccer athletes (3 men, 5 women, mean age = 19.3 years (men) and 19.4 years (women), mean height = …

Influence Of Various Pre-Exercise Routines On Vertical Jump Performance, Vladislav Milanovic

Masters Theses

The purpose of this study was to determine and compare the influence of three different pre-exercise routines (jogging, proprioceptive neuromuscular facilitation in addition to jogging (PNFJ), and whole body vibration (WBV)) on vertical jump (VJ) performance. Twelve physically active, collegiate males between the ages of 18-24 years were recruited to participate. The subjects performed four VJ tests on four nonconsecutive days with a control VJ being performed on the first day and the three remaining VJ performed after each of the pre-exercise routines that were conducted in random order. The jogging pre-exercise routine lasted 5 minutes at light to moderate …

Metagenomic Analysis Of The Lacrimal Caruncle Region Of Canis Lupus Familiaris Using Miseq Technology, Seun A. Ikugbayigbe

Masters Theses

The identification of ocular microbiota may allow early diagnosis and treatment strategies against eye diseases and disorders with canines. However, clinical microbial identification has been limited to cloning and conventional culture-based studies, which typically underestimate community diversity. In this report, lllumina MiSeq analysis of the 16S rRNA gene was used to examine the microbiome of the lacrimal caruncle region from five healthy dogs. The breeds sampled were a Golden Retriever (Dog A), a Weimaraner (Dog B), a Shih Tzu mix (Dog C), a Yorkie mix (Dog D), and a Dachshund (Dog E). MiSeq analysis revealed a total of 370 operational …

Effects Of Postactivation Potentiation Succeeding Glute Bridge Exercise On Multiple Jump Performance In Recreationally Trained Individuals, Anthony Warpecha

Masters Theses

The purpose of this study was to test multiple jump performance subsequent to glute bridges performed with and without whole body vibration (WBV). Multiple jump performance was assessed via a four-hop test to examine average jump height, ground contact time, and the explosive leg power factor. Twenty recreationally active subjects, ages 18-42, with more than one year of consistent recreational resistance training experience participated. Prior to testing, one familiarization session took place that involved explanation of procedures. This included practice of glute bridging on the vibration plate and the multiple hop performance test. After the familiarization session, two testing sessions …

The Functional Role Of Leaf Nutrients In An Old-Field Successional Community, Kirstin I. Duffin

Masters Theses

In functional ecology, traits that capture aspects of plant performance are used to understand how organisms interact with their environment. Leaf nutrients are an example of a functional trait that directly links to plant metabolic processes and therefore may describe plant assemblage dynamics. Multivariate leaf nutrient analyses may be used with other functional traits to understand ecological strategies because they are a direct measure of leaf metabolic processes and can describe nuances in plant allocation patterns. In this thesis, I explored (1) whether a suite of leaf nutrients (carbon, nitrogen, phosphorus, potassium, calcium, and magnesium) was related to plant growth …

Trophic Interactions In A Semiaquatic Snake Community: Insights Into The Structure Of A Floodplain Food Web, Cynthia M. Carter

Masters Theses

Food webs provide a useful conceptual framework for evaluating the relationships that exist within ecological systems. Characterizing the interactions within these webs can improve our understanding of how communities are structured and what mechanisms stabilize them. Untangling these interactions can be an intractable problem in complex systems and insights gained from conventional methods are often accompanied by inherent sources of bias. This study used stable isotope analysis, an alternative to traditional methods, to investigate the roles and relative contributions of consumers at the top of a food web to community structure and stability. I compared the niche parameters of five …

Detecting Diesel Particulate Matter Using Real Time Monitoring Under The Influence Of An Exhaust Fan System, Yasir Helal Alghamdi

Masters Theses

"Diesel Particulate Matter (DPM) is a complex mixture of diesel exhaust gas that consists of carbon, ash, metallic abrasion particles, sulfates and silicates. The diesel soot particle includes a solid core made of elemental carbon, and organic carbon compound attached to the surface of the soot particle. The main source of DPM is diesel exhaust gas. The National Institute for Occupational Safety and Health (NIOSH) and the Environmental Protection Agency (EPA) have determined that DPM is the source of most of the emissions of carbon monoxide, carbon dioxide, oxide of nitrogen, and hydrocarbons in underground coal, metal and non metal …

The Impact Of Aging And Estrogen Therapy On Synaptic Mitochondrial Bioenergetics In An Alzheimer's Mouse Model, Eric W. Hendricks

Masters Theses

Mitochondrial dysfunction, such as reductions in ATP production via oxidative phosphorylation, is a recognized pathological symptom of neurodegenerative diseases including Alzheimer's disease (AD). One of the known risk factors for the development of AD is the inheritance of a specific genotype of a lipid transporting protein known as apolipoprotein E (apoE). How apoE contributes to the development and progression of AD is poorly understood. The purpose of this study was to determine if a relationship exists between mitochondrial dysfunction and genetic predisposition to AD via apoE. Mitochondrial bioenergetics and enzymatic activity were investigated in an AD mouse model (apoE KO) …

Sensitivity Of Cellular Oxidative Damage To Biosynthetic Rate And Metabolic Rate, Palihawadana Walauwe Kamalika Kaushalya Amunugama

Masters Theses

"The relationship between energy expenditure and longevity is a long standing question in aging studies. The empirical results have contradictory effects on the existing theories. A theoretical model and an experimental test of it were presented, revealing the detailed tradeoffs between metabolic rate, biosynthetic rate and cellular damage level. The dissipative mechanisms of oxidative metabolism cause various forms of cellular damages. To counteract the accumulation of damage, organisms have evolved highly efficient maintenance mechanisms. If there is no other energy demand possess, then most of the cellular damage would be repaired, regardless of how metabolic rate varies. However, the repairing …

Fern Community Reassembly In Secondary Forests Of Puerto Rico: Predictors, Complexity, And Niche Model Assessment, Thomas J. Schmidt

Masters Theses

Approximately 94% of Puerto Rico’s forests were converted into agricultural systems by 1950. Since then, extensive abandonment of agricultural land has resulted in a considerable amount of forest regeneration throughout the main island. Ferns are a major non-woody component of oceanic, tropical island forests comprising up to seventy percent of the flora. Consequently, the composition and community structure of ferns may be indicative of the relative richness of these secondary forests. I used Maximum Entropy (Maxent), a widely-used mathematical tool for distinguishing suitable versus unsuitable fern niche space, along with ENMTools, a tool that assists Maxent with proper model selection, …

Genetic Health And Population Viability Of Reintroduced American Marten In Michigan, Tamara L. Hillman

Masters Theses

American marten (Martes americana) were extirpated from Michigan’s Lower Peninsula (LP) in 1911, and subsequently from the Upper Peninsula (UP) in 1939 due to habitat loss and unregulated trapping. The species was later reintroduced in the UP in the mid-1950s, and to the LP in the mid-1980s. Previous research has determined the small founding sizes used in the LP reintroductions have resulted in losses of genetic diversity, while research in the UP has produced discordant results concerning the effects of the reintroduction methods on genetic health and population structure. Since past research of marten in the LP, no …

Predictive Modeling Of Floral Species Richness In Michigan Prairie Fen Communities, Nichole R. Mason

Masters Theses

Prairie fens contain high levels of floral biodiversity, including 19 state threatened or endangered plant species, and are classified as rare and vulnerable communities by the Michigan Natural Features Inventory. The objective of this thesis was to develop multiple-regression (MR) models that reliably predict total, native, and invasive floral species richness for use by conservation organizations. Floral biodiversity surveys were conducted in eight southern Michigan prairie fens during the 2012 growing season. Simple linear regressions between fen size and biodiversity were used to optimize sampling strategy and effort (i.e., number of transects and plots per transect) in surveys conducted in …