Life Sciences Commons^™

Food Security Through Disaster Planning In Tanzania: How Agriculture-Based Disaster Preparedness Can Improve Food Security In Tanzania, Savannah A. Emerich

Masters Theses

In Tanzania, half the population lives below the poverty line and suffers from food insecurity. Of the population, women and children primarily suffer from malnutrition due to food insecurity. For Tanzania, the problem is so severe that some women do not name their newborns for weeks or months due to their unlikely survival. This problem worsens for those who have a significant reliance on agriculture and live in the nation’s rural areas. These rural communities face more traumatic burdens when floods and droughts destroy crops, fields, and tools and ruin livelihoods. A solution has not been implemented thus far due …

Comparing Macroinvertebrate Total Abundance And Total Biomass On Five Substrate Types From Upstream To Downstream On The North Branch Of The Au Sable River, Paul David Dingman

Masters Theses

The North Branch of the Au Sable River is located in the northern lower peninsula of Michigan and is known for prolific hatches of Ephemeroptera (mayflies), Trichoptera (caddisflies), and Plecoptera (stoneflies). Macroinvertebrates play an important role in processing and recycling organic material in rivers and are a valuable food source for trout. In 2018, anglers were reporting catching fewer numbers of brook and brown trout (Salmo trutta and Salvelinus fontinalis). The Michigan Department of Natural Resources (MDNR) determined significantly lower abundance than the historical average. We hypothesized that trout abundances were lower due to a lack of prey …

The Flavoprotein Rcla Is A Hypothiocyanous Acid Reductase, Irina Chapman

Masters Theses

Reactive chlorine species (RCS) - hypochlorous acid (HOCl), hypothiocyanous acid (HOSCN), and chloramines - are powerful antimicrobial oxidants generated by the innate immune system. Bacteria developed defense mechanisms against RCS, which are highly toxic for invading pathogens. One such defense system involves RclA, an enzyme from the flavin disulfide reductase family, which is massively upregulated upon exposure of Escherichia coli to RCS. Recent research suggests that RclA functions as a Cu(II) reductase to provide resistance against RCS. However, the exact mechanism used by RclA was subject to debate. In this study, we demonstrate that RclA is unlikely to function as …

Does Epa Cause A Decrease In Inflammation Of Bend.3 Cells Through Ffar4?, Clay J. Weidenhamer

Masters Theses

Atherosclerosis is an inflammatory disease initiated by low and oscillatory shear stress on the endothelium. The inflammatory process recruits leukocytes to the vessel wall by expression of the adhesion molecule VCAM-1. Activation of the NF-κB inflammatory signaling pathway is responsible for the increase in VCM-1 expression. Omega 3 FAs, such as EPA, reduce the risk of atherosclerosis by decreasing this inflammatory response. The pathway by which omega 3 FAs is proposed to inhibit inflammation includes activating FFAR4 to decrease NF-κB activation thereby reducing expression of adhesion molecules. We hypothesized that treatment of endothelial cells with 30 μM EPA would decrease …

Effects Of Shoreline And Watershed Development On Eastern Lake Michigan Drowned River Mouth Ecology, Megan M. Mader, Carl R. Ruetz Iii

Masters Theses

Lake Michigan’s drowned river mouths (DRM) are hydrologically unique systems with riverine and large-lake influences that create biologically diverse ecosystems. Serving as focal points for human development due to the ecosystem services they provide, DRMs have experienced a history of industrialization, urbanization, and are now moving towards an era of rehabilitation. Today, DRM shorelines have been hardened, their riparian zones have been severely altered, and their watersheds exist on a latitudinal gradient of less to greater anthropogenic stress (agriculture and development). The main goal of this study was to understand the current ecological state of DRMs related to anthropogenic development …

Immune Cell Profile In The Epididymis Of Leptin And Leptin Receptor Knockout Mice, Hilary Skalski

Masters Theses

Genetically-modified mice lacking the leptin hormone (Ob) or leptin receptor (Db) have reduced testosterone and decreased sperm production leading to infertility. Leptin receptors are also expressed by immune cells, suggesting leptin can regulate immune responses. Recent studies have focused on identifying the immune cells present within the epididymis and found cells expressing markers typically associated with dendritic and macrophage cells. These cells likely function against infection and to induce tolerance to sperm. The effects of leptin on the immune system and the presence of immune cells within the epididymis raises the possibility that alterations in the immune cell profile and/or …

Assessing The Short-Term Effects Of Translocation On Freshwater Mussels: Is Habitat Or Water Quality More Important?, Joshua D. Arnold

Masters Theses

Freshwater mussels (Order: Unionida) are very important to the function of aquatic ecosystems and are typically indicators of good water quality. They provide a valuable link between the water column and the benthic substrate in which they live and are a valuable food resource for many species of animals. However, most species native to North America are currently threatened with extinction, to the point that more than 70% of native freshwater mussels are listed as either threatened or endangered at the state or federal level. The cause of this decline can be attributed to historical over exploitation, habitat alteration, and …

Pedigree And Molecular Assessment Of Relatedness In A Captive Chimpanzee Population, Francesca Golus

Masters Theses

As a crucial component of biodiversity, genetic diversity contributes to variability among individuals, allowing populations of endangered species to be resilient in the face of changing environmental conditions. Zoological institutions have become a cornerstone of conservation efforts and a refuge for endangered species given threats imposed on wild populations by climate change, habitat fragmentation and degradation, and overexploitation. Chimpanzees (Pan troglodytes) are an endangered species experiencing drastic population decline in the wild, yet are common residents in zoos and wildlife sanctuaries. To sustain long-term, genetically viable populations of species of concern, zoos use studbooks and paternity testing to …

Retinal Regeneration In A Genetic Mouse Model Of Glaucoma, Joshua Paris

Masters Theses

Glaucoma is a degenerative retinal disease characterized by progressive loss of retinal ganglion cells (RGCs). Previous studies have shown that application of a specific α7 nicotinic acetylcholine receptor agonist, PNU-282987 (PNU), onto the murine retina induces neurogenesis of numerous retinal cell types, including RGCs. The aim of this study is to characterize the short-term and long-term effects of PNU in a glaucoma model. The effects of PNU were analyzed in a DBA/2J mouse model that auto-induces a glaucoma-like condition in adulthood. These mice manifest an elevated intraocular pressure (IOP) starting at 6 months, followed by loss of RGCs. To assess …

Effects Of Traditional And Microbially-Focused Restoration Techniques On Soil Communities In Tallgrass Prairies, Zachary J. Whitacre

Masters Theses

Tallgrass prairies have virtually disappeared in many parts of their former range due to the conversion of this ecosystem to farmland. In more recent years there have been efforts to restore these prairies on reclaimed agricultural land. However, these restored prairies do not resemble their remnant counterparts in many ways, such as in soil microbial community composition and metrics related to carbon storage. In Chapter 1, I show that bacterial communities in a restored prairie and an adjacent remnant prairie in southwest Michigan differ in their immediate and longer-term responses to prescribed fire, a commonly used prairie restoration and maintenance …

Strong Women Breaking Ground: Roles Of Women In Agriculture In Michigan, April L. Shirey

Masters Theses

Agriculture in Michigan is changing. While the number of farms and farmers continue to decrease, women are increasingly taking on the role of farmer instead of the “farmer’s wife”. The number of female producers increased from 8,275 to 26,059 where the number of producers in Michigan decreased from 56,014 to 47,641 from 2007 to 2017 (USDA, 2007, 2017). Women are becoming the face of farming in Michigan, yet little research examines the impacts of these shifts. In this research, I conduct semi-structured interviews with female farmers throughout lower Michigan beginning in the summer of 2020 to learn more about these …

Effects Of Am Fungi From Conventional And No-Till Michigan Crop Fields On Plant And Soil Health, Derek Bennett

Masters Theses

Centuries of conventional till (CT) management in agriculture has depleted soil organic matter (SOM) by over 50%. While only comprising 5% in most soils, SOM provides soil with fertility and productivity. To compensate for SOM depletion, producers have been forced to increase their reliance on fertilizer and irrigation to maintain yields. In the coming decades, climate change is expected to challenge food production and threaten an already fragile system. With no remaining land left to cultivate, conservation management strategies such as no-till (NT) look to restore SOM and increase the resilience of food production for an ever growing, increasingly food …

Evaluation Of Α-Pyrrolidindovalerophenone (Α-Pvp) And Its Isomers In Male And Female Sprague-Dawley Rats Trained To Discriminate Mdpv, Kaley Cargile

Masters Theses

Recreational use of synthetic cathinones, 3,4-methylenedioxypyrovalerone (MDPV) and α‐pyrrolidinovalerophenone (α‐PVP), has become increasingly popular, thus prompting characterization of their behavioral and neurochemical effects. MDPV has been studied for several years now, though there is still much unknown about α‐PVP, and its isomers. The primary objective of this study was to characterize the discriminative effects of α‐PVP and its isomers in comparison to MDPV. Male and female Sprague-Dawley rats were trained to discriminate 0.5mg/kg MDPV from saline injections under a fixed ratio 20 (FR 20) schedule of food reinforcement. Substitution tests were conducted with MDPV (0.05-0.5mg/kg), α‐PVP (0.05-0.5mg/kg), (S)-α‐PVP (0.05-1mg/kg) and …

Fecal Findings: Investigating Eastern Massasauga Rattlesnake Diet Using Dna Metabarcoding, Alyssa Swinehart

Masters Theses

Characterizing the diet of imperiled species using minimally invasive methods is crucial to understanding their conservation requirements. DNA metabarcoding methods have been used to characterize the diet primarily in mammalian systems, while reptiles are heavily underrepresented in this literature. Here, we apply a DNA metabarcoding approach to study the diet of the eastern massasauga rattlesnake (Sistrurus catenatus); a Federally Threatened snake found throughout the Great Lakes Region. Eighty-three fecal samples collected across 10 different massasauga populations located in Michigan were sequenced. We use universal metazoan primers and develop a host-specific oligonucleotide blocker to uncover the full potential diet …

Spatial And Demographic Patterns Of Two Threatened Turtle Species In An Urban Environment, Carly E. Brouwers

Masters Theses

Urban development is a global threat to native wildlife. The process of urbanization reduces and degrades the useable habitat of a region, and creates novel “urban ecosystems” that possess new threats and stressors to local species. Turtles are one of the most threatened vertebrate groups worldwide, and are particularly at risk of decline in urban ecosystems due to reduced nesting success, increased road mortality events, altered movement patterns, and increased predation rates. Eastern box and Blanding’s turtles are two at-risk turtle species in the state of Michigan, USA, primarily due to land use change. Presently, there are urban populations of …

Evaluation Of Lake Sturgeon (Acipenser Fulvescens) Spawning Success On An Artificial Reef Constructed In The Kalamazoo River, Michigan, Jason P. Lorenz

Masters Theses

Since lake sturgeon Acipenser fulvescens monitoring began on the Kalamazoo River in 2009, there has been no documentation of survival past the egg stage. Spawning has occurred directly below Calkins Dam and, in 2016, an artificial spawning reef was constructed 275-m downstream to improve habitat and increase survival to the larval stage. The site was monitored with egg mats and larval drift nets to document evidence of successful natural reproduction of lake sturgeon one year prior to construction (2016) and three years post-construction of the artificial reef (2017-2019). Fertilized lake sturgeon eggs were captured all four years of the study …

Use Of Imaging Flow Cytometry To Assess The Hematology Of Eastern Massasauga Rattlesnakes (Sistrurus Catenatus), Jennifer H. Kovach

Masters Theses

The federally threatened eastern massasauga rattlesnake is faced with many threats, including the newly recognized snake fungal disease. Unfortunately, the immune response to the disease is unknown. Leukocyte profiles are used as health assessment tools for many species, and imaging flow cytometry can be used as an application to characterize leukocyte profiles. Our goal was to use imaging flow cytometry to identify leukocyte profiles of eastern massasauga rattlesnakes and determine differences in profiles between sexes, reproductive status, ages, location, and individuals with clinical signs of snake fungal disease. Blood samples from 201 individuals were collected from ten locations throughout the …

Genome-Wide Characterization Of Lateral Organ Boundaries Domain Genes In Sunflower (Helianthus Annuus L.), Saleh M. Aljurbua

Masters Theses

Lateral Organ Boundaries (LBD) gene family encodes transcription factors found only in plants with essential roles in growth, development, and response to biotic and abiotic factors. The sunflower (Helianthus annuus L., Asteraceae family) LBD genes were studied using different in silico and phylogenetic approaches including four major steps: gene identification, phylogenic analysis, structural analysis, and expression analysis. Sixty-four sunflower LBD genes (HaLBDs) were identified using query search of all Arabidopsis LBD genes against sunflower genome (version r1.2) followed by LBD domain sequence verification. A phylogenetic tree was reconstructed using MEGA-X program. The identified genes were phylogenetically classified into two main …

Effects Of Emerging Contaminants On Centrarchidae And Catostomidae In Midwestern Rivers: A Multiple Biomarker Approach, Camden Garret Nix

Masters Theses

Natural habitats of fish are gradually declining due to land use and pollution caused by industrial wastes, intensive agriculture and contamination from sewage systems. The United States has 14,780 wastewater treatment facilities that discharge effluent into navigable waters, and in some cases these discharge waters represent a considerable proportion of the water system flow. These activities make it critical to discern the effects of pollutants that are entering our waterways at a consistent rate, such as 17β-estradiol (E2) and nickel, on aquatic organisms. To determine the effects of 17β-estradiol on endocrine disruption in fish, Bluegill Sunfish (Lepomis macrochirus) …

Effects Of Wastewater Effluent On Fish Fin Morphology Of Larval Fathead Minnows (Pimephales Promelas) And Swimming Performance Of Smallmouth Buffalo (Ictiobus Bubalus), Seth Michael Bogue

Masters Theses

The Sangamon River flows across central Illinois and is mostly free flowing except in the city of Decatur where it is impounded to form Lake Decatur. The Sanitary District of Decatur (SDD) is located approximately three miles downstream of the Lake Decatur dam. For a significant portion of the year, the stretch of the Sangamon River downstream of SDD is dominated by effluent due to limited discharge from the Lake Decatur dam. This has resulted in heavy nutrient loading, most notably phosphorus, and elevated levels of nickel and other compounds in the downstream section of the river. Smallmouth buffalo ( …

Genetic Relatedness Can Alter The Strength Of Plant-Soil Feedbacks, Kelly M. Clark

Masters Theses

Intraspecific variation may play a key role in understanding the relationships between plants and their interactions with soil microbial communities. The effects that soil-microbes have on individuals can venerate variation across individuals in their responsiveness. I explored how relatedness alters plant-soil feedbacks in established Solidago altissima clones grown in a common garden. Seedlings of known parentage were inoculated with soils from the maternal, paternal, or unrelated clones and compared to autoclaved control inocula. I found that the soil inocula generated from S. altissima had an overall negative effect of seedling biomass. Furthermore, seedlings inoculated with maternal or paternal soils experienced …

The Relationship Between Relative Muscular Strength And Joint Mobility In Firefighters, Samuel C. Nozicka

Masters Theses

Firefighters job requirements consist of running upstairs, climbing ladders, ceiling breach and pull, carrying equipment, forcible entry, dragging hoses, raising ladders, and rescue of patrons all while wearing heavy protective equipment that limits their mobility (Park et al., 2015). The purpose of this study was to examine the relationship between relative strength and mobility within the population of firefighters. The subjects were volunteers consisting of twelve male firefighter ranging in age from 25-52 with the mean age of 37.7 ± 7.7 years. Leighton Flexometer was used to measure joint range of motion (ROM) in different active movement patterns (Leighton, 1966). …

The Gene Expression Patterns Of Phanerochaete Chrysosporium And Trametes Versicolor On Diverse Lignocellulosic Feedstocks, Noor Osama Alabbasi

Masters Theses

Plant biomass is considered to be an important future starting material for fuels and chemicals, thereby decreasing our reliance on fossil fuels. While direct combustion continues to be a source of fuel, the generation of liquid fuels from plant biomass for transportation has proven to be challenging. Among the limiting factors for conversion of this material to biofuels is the recalcitrant nature of lignocellulose, the primary component of non-starch plant biomass. A strategy to overcome this dilemma is to directly or indirectly use the enzymes from white-rot fungi, which have evolved a unique ability to deconstruct lignocellulose. However, the biochemical …

Preclassic Cultural Eutrophication Of Lake Petén Itzá, Lowland Guatemala, By The Early Maya Of Nixtun-Ch'ich', Brooke A. Birkett

Masters Theses

"Paleolimnological evidence indicates the ancient Maya transformed lowland terrestrial ecosystems by felling forest vegetation to construct large civic-ceremonial centers and expand agriculture. The effects of prehistoric Maya land alterations on lake trophic status, however, remain poorly understood. We analyzed a 515-cm-long sediment core from Lake Petén Itzá, lowland Guatemala, to infer paleoenvironmental changes resulting from Maya occupation of the riparian archaeological site of Nixtun-Ch'ich'. Substantial increases in charcoal and fecal stanol concentrations indicate Maya occupation of the Candelaria Peninsula by the late Early Preclassic period beginning ca. 1400 cal yr Before the Common Era (hereafter BCE), despite scant archaeological evidence …

The Validation Of Predicted Biological Age Of Drosophila Melanogaster From Combined Statistical Modeling, Lauren Francis

Masters Theses

"Sleep is essential for maintaining a healthy body and mind and is associated with aging and aging related diseases. There are individual differences in fly as well as human sleep behavior and lifespan. Between and within individuals, sleep varies in characteristics including consolidation, rhythmicity, continuity, duration, and more. Various evidence in the literature suggests there are many molecular pathways involved with aging and they may be different for individuals. Our research is interested in a possible restorative mechanism of sleep and the ramifications of that mechanism to aging. We have developed two predictive models of aging using the fruit fly …

Immediate Response Of Black Basses To Dam Removal Is Determined By Habitat Preference, Reuben Frey

Masters Theses

Dams affect the abundance of fish species in lotic systems by altering flow regime and available physical habitat. Removal of dams may mitigate these effects and generate a change in species abundance and fish community structure. Understanding the effects of dam removal on gamefish species is essential in making management decisions. I investigated how populations of Smallmouth Bass (Micropterus dolomieu), Spotted Bass (M. punctulatus), and Largemouth Bass (M. salmoides) were affected by the presence and removal of two low-head dams in a Midwestern river system. I used data collected during fall and spring from …

Development Of Fluorescence Microscopy Approaches To Study Subcellular Protein Transport And Enzymatic Activity, Anchal Singh

Masters Theses

Understanding the subcellular localization of proteins and their activity is important in understanding their normal function in eukaryotic cells. Fluorescence cellular imaging techniques can selectively and sensitively visualize subcellular biochemistry. Using this approach, two different methods were employed in this thesis. The first focused on studying protein import into peroxisome and the other on monitoring the activity of an endoplasmic reticulum (ER)-localized enzyme, human carboxylesterase 1 (CES1).

Peroxisomes are mainly known as the center for long chain fatty acid b-oxidation as well as the production and detoxification of hydrogen peroxide. Proteins which are needed in the peroxisomes are encoded in …

Xylan-Derived Carbon Nutrient Acquisition By Caulobacter Crescentus, Rhoda Ekuama Inkoom

Masters Theses

Bacterial decomposition of xylan and uptake of xylan-derived carbon compounds require a combination of enzymes, proteins, and complex molecular mechanisms. Bacteria have evolved molecular mechanisms for its utilization depending on its environment and source of xylan. Caulobacter crescentus, a Gram-negative bacterium which lives in an aquatic oligotrophic environment, exhibits asymmetric division which is an adaptive feature for its nutrient poor habitat. Although aromatic hydrocarbon and cellulose-derived mono-, di- and oligosaccharide utilization has been reported in literature, its xylan-derived carbon compound utilization is yet to be characterized. In this study, C. crescentus utilizes xylan-derived monomers arabinose, xylose and acetate as …

Flexibility: The Forgotten Component Of Fitness: Eccentric Exercise Vs. Static Stretching To Improve Flexibility, Zach Cox

Masters Theses

The purpose of this research is to assess the effects of eccentric exercise as well as static stretching on flexibility following a six-week training intervention. It also compared the effects to those of a static stretching program. The hypothesis is that eccentric training will show larger enhancements in flexibility in sagittal plane movements of the hips and knees in comparison to a static stretching program. Training sessions were done three days per week and had a 24-hour rest period minimum between them. The exercises for the eccentric group were as follows: standing hip extension, standing split, straight leg lowering, and …

Chemical Diversity And Antimicrobial Properties Of Spike-Rush Species And Related Genera (Genus Eleocharis, Family Cyperaceae), Yiru Zhang

Masters Theses

The focus of this research was to explore the chemical diversity and antimicrobial properties of Eleocharis dulcis. By using methanol as a solvent to extract phytochemicals, qualitative comparisons were made between HPLC chromatograms from Eleocharis species and other genera. Meanwhile, this study also examined the antimicrobial properties of phytochemicals extracted from various tissues of Eleocharis dulcis in two different solvents (water or methanol) against two Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli) and two Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) using a disc diffusion assay. The results suggested that the extracts had limited antimicrobial properties at up to 100 …