Life Sciences Commons^™

Effects Of Nixtamalization On Maize Processing In Malawi, Annica Cooper, Frost Steele

Journal of Undergraduate Research

Malawi is a small country in southeastern Africa. The main crops that rural Malawians grow for food are maize, legumes, and dark, leafy greens. Due to this restrictive diet, many Malawians are at risk for several nutritional deficiencies. In a survey conducted of Malawian families at the School of Agriculture for Family Independence (SAFI), families reached an average of only 18.3% of their calcium needs. A calcium deficiency, especially early in life, can lead to low bone mass and low vitamin D levels, which leads to rickets or osteomalacia. An ancient Mesoamerican tradition called nixtamalization turns maize into a great …

Combating Anemia And Other Iron Related Deficiencies Through Iron- Efficiency In Soybeans, Miranda Ruth, Bryan Hopkins

Journal of Undergraduate Research

Plants uptake iron from the soil in a variety of ways. Dicots, like soybeans, primarily exude hydrogen (H+) ions and chemical reductants from their roots, which acidify the root zone and reduce Fe³⁺ to Fe²⁺ that can then be utilized by the plant (Brown and Jolley 1989). Iron efficiency in soybeans results in increased dietary iron for those eating the beans. Anemia, which is due to a dietary lack of iron, is an extremely serious health problem that affects much of the world, especially developing countries. Globally, over 30% of the world suffers from this lack of dietary …

Modelling Climate Suitability For Ephedra Viridis In Space And Time, Anne Thomas, Richard Gill

Journal of Undergraduate Research

Climate change is expected to decrease soil moisture in the sensitive, water-limited ecosystems of America’s southwestern deserts, leading to shifts in plant distributions and altering ecosystem function. Studies have already documented the loss of desert grasses and the expansion of desert shrubs on the Colorado Plateau near Canyonlands National Park.i The objective of this work is to identify the climatic and biophysical factors influencing the distribution of an evergreen shrub, Ephedra viridis, and its expansion in plot-scale studies over the past 30 years.ii A gymnosperm and member of the plant division Gnetophyta, this shrub is unique because of its …

Alzheimer’S Risk Genes And Pathways Of Expression, Samantha Jensen, John Kauwe

Journal of Undergraduate Research

Although Alzheimer’s disease (AD) has been the subject of research for nearly 100 years, these decades of research have not led to a unified understanding of the mechanisms behind the disease pathology1 ,2. Recent improvements in whole genome sequencing have made it possible for researchers to find a large number of mutations implicated in AD. Due to a high frequency of false positives and confounding factors, many mutations being investigated for therapies may not even have a causative effect on Alzheimer’s expression. Without concrete knowledge of the interactions of the proteins and genes implicated in the disease, drug development has …

Development Of A Model For Adult T Cell Leukemia In Htlv-1 Infected Humanized Mice, Matthew Smith, Dr. Brad Berges

Journal of Undergraduate Research

Millions of people in the world are infected with human T-lymphotropic virus type-1 (HTLV-1). As the name states, this virus attacks the T cells of the infected organism. Though many of these individuals will remain asymptomatic throughout life, a small percentage will develop adult T cell leukemia/lymphoma (ATLL), a rare and understudied form of cancer. The latency period, or amount of time between infection and detection of cancer, is generally 20-40 years before infected patients begin developing cancer.

Phenome-Wide Association Study Of Y Chromosome Genetic Markers, Matthew Durrant, Mary Davis

Journal of Undergraduate Research

The purpose of this project was to analyze genetic variants on the Y chromosome for significant association with various diseases. In Dr. Mary Davis’ lab we gained access to a unique data set that allows us to analyze many single-nucleotide polymorphisms (SNPs) on the Y chromosome. This was a hypothesis-generating study that has the potential to revolutionize the currently accepted hypothesis that genetic variants on the Y chromosome do not play a significant role in common disease.

Cellular Mechanism Of Thymidine Kinase 1 Secretion, Wei Meng, Kim O'Neill

Journal of Undergraduate Research

Cancer remains the second most common cause of death in the US, accounting for nearly 1 of every 4 deaths. Studies have shown that the early detection of cancer leads to better patient prognosis and a greater five-year survival rate. [1] Diagnostic and prognostic markers play a key role in classifying tumors and determining the best treatment plan for a patient. Among these biomarkers, Thymidine kinase 1 (TK1) has been studied extensively, primarily as a diagnostic biomarker for a variety of cancer types. TK1 plays a role in regulating the intracellular thymidine pools throughout the cell cycle, and thus regulates …

If The Beach Boys Were Diabetic, They Would Sing About Good Vibrations!, Peterson Jordan, Brent Feland

Journal of Undergraduate Research

One in every four Americans suffer from Diabetic Neuropathy in the lower extremities. This research has a lot to do with the pain and anguish of one fourth of the American people. This pain can cause depression and other mental illnesses, because it reduces the patient’s physical activity by a land slide. This can be traumatic for the patient and the family. This neuropathy in the lower extremities causes nerve damage in the foot and leg area. Doctors have found that it is due to a lack of blood to the nerves, causing the nerve endings to slowly die. This …

Soil Water And Temperature Effects On Resilience Resistance Of Sagebrush Steppe Plant Communities, Trevor Gruwell, Bruce Roundy

Journal of Undergraduate Research

Project Purpose-The purpose of this project was to evaluate the effects of environmental factors on overall resistance and resilience of the sagebrush steppe community. Resistance is defined as a system’s ability to avoid change after a disturbance has taken place and resilience is defined as the ability of a system to return to its natural state after a disturbance has taken place.

Mono-Associated Gnotobiotic Animal Meta-Genome Wide Association R Package, Corinne Penrod, John Chaston

Journal of Undergraduate Research

Animal-associated bacteria fundamentally influence the normal function and behavior of their hosts, and understanding the genetic basis for these effects is an ongoing challenge. The traditional approach to understanding phenotypic effects of genes by mutant analysis is to perform a genetic screen. MAGNAMWAR is an R package that provides a simple and effective gene function predicting pipeline to be used as a faster alternative to a genetic screen. It uses meta-genome wide association (MGWA) and bacteria genotype-host phenotype relationships to predict bacterial gene function. Proven to be an effective gene predictor in work done with Drosophila melanogaster, this pipeline …

Expression Changes Of Dopamine Type 2 Receptor On The Surface Of Human Monocytes And Lymphocytes After Exposure To Dopamine In Vitro., Brandon Thomas Garcia, Sandra Hope, Scott Steffensen

Journal of Undergraduate Research

Dopamine (DA) is a neurotransmitter in the brain that plays a role in several functions such as reward, motivation, and dependence. The imbalance of DA in the central nervous system (CNS) can lead to several debilitating diseases like Parkinson’s Disease, Alzheimer’s, and Schizophrenia as well as drug dependence. Levels of DA in the brain are often determined by measuring the expression of Dopamine type 2 (D2) receptors by positron emission topography (PET) scans in the Nucleus Accumbens (NAc). This test which is often expensive and unavailable to many people without access to hospitals or regular healthcare.

Development Of Chimeric Antigen Receptor T Cells To Target Cancer, Justin Crandall, Dr. Scott Weber

Journal of Undergraduate Research

Cancer negatively affects the lives of millions of individuals, and remains a difficult ailment to treat. In 2014, approximately 585,720 deaths and 1,665,540 new cases of cancer were reported in the USA alone.1 Treatment of cancer is challenging due to cancer mutating to combat the body’s immune system. An example is shown in major histocompatibility complexes (MHCs) located on the surface of nearly every cell. These are patrolled by T cells to ensure that cells are properly functioning and healthy. With cancer however, these become downregulated allowing the cancer to grow and proliferate since T cells are unable to detect …

Is Hdac1 Mediated Β-Cell Proliferation Dependent On Decreased P15 Expression?, Kyle Kener, Jeffery Tessem

Journal of Undergraduate Research

The body maintains normoglycemia through pancreatic β-cells which sense elevation in circulating glucose levels and secrete insulin to maintain the correct blood glucose concentration. A decrease in functional pancreatic β-cell mass leads to Type 1 or Type 2 diabetes. Each form has different initial causes, but both forms ultimately result in decreased functional β-cell mass. Functional β-cell mass is defined as the rate of glucose stimulated insulin secretion multiplied by the total β-cell mass. The total β-cell mass is a function of cellular proliferation and death rates. β-cells have a very low proliferation rate, therefore determining the mechanisms that induce …

Determining The Mechanism Of Cocoa-Derived Epicatechin Enhancement Of Glucose Stimulated Insulin Secretion, Benjamin Bitner, Jeffery Tessem

Journal of Undergraduate Research

Due to recent increases in incidence, diabetes is now classified as a global epidemic. Diabetes results from the eventual destruction of β-cell mass, which causes decreased insulin secretion. Increasing functional β-cell mass may provide a cure for diabetes. A previous study has shown that oligomeric procyanidins were effective in preventing weight gain, fat mass, impaired glucose tolerance, and insulin resistance in a mouse obesity model¹. This was the first long-term feeding study to examine the activities of cocoa constituents on diet-induced obesity and insulin resistance.

Investigating Species Boundaries And Taxonomy In The California Pincushion Plants, Theresa Saunders, Leigh Johnson

Journal of Undergraduate Research

Species in the genus Navarretia are annual wildflowers found almost exclusively in California. The Navarretia atractyloides group consists of just two species, N. atractyloides and N. hamata, that are more similar to each other morphologically than to any other Navarretia species. The most recent taxonomic investigation of these two species distinguishes between them based on bract shape, stamen attachment to the corolla, and whether the style is included and anthers included to slightly exserted from the corolla tube versus style exserted and anthers long exserted (Day, 1993). However, we have noticed that some specimens that seem to be …

Creation Of Twelve Member Plasmid Library For Promoter Swapping To Control Chromosomal Gene Expression In E. Coli, Chris Nielson, William R. Mccleary

Journal of Undergraduate Research

Metabolic engineering is becoming a very important area of research, allowing researchers to harness metabolic pathways to either eliminate or synthesize desired compounds. Understanding metabolic pathways by altering expression of proteins involved in the pathway helps to uncover thermodynamic bottlenecks which render certain pathways inefficient or infeasible. Altering expression of genes through the use of promoter swapping is a useful research technique used to understand important metabolic and physiologic pathways. Engineered promoters contained in plasmids can be inserted into chromosomal DNA through the utilization of promoter swapping to alter the expression of a given gene in order to better understand …

Velvets: Software For Optimizing Velvet Input, Donald Ford, John Chaston

Journal of Undergraduate Research

Genome assembly is an important tool used to obtain more complete representations of entire genomes. Sequencing techniques, such as shotgun sequencing, are able to generate short pieces of a genome known as reads. Genome assemblers use these reads in order to produce larger genome sequences, known as contigs. This is done by computing how these reads overlap with each other. The ultimate goal is to create a single contig which represents an entire genome, though usually genome assemblers fall short of this goal.

Transition From Cardiovascular Disease To Cancer As The Leading Cause Of Death In Us States, 1999-2014, Michael C. Harding, Evan Thacker

Journal of Undergraduate Research

In 1900 the three leading causes of death in the United States were pneumonia and influenza; tuberculosis; and gastritis, enteritis, and colitis.¹ However, through a combination of improved sanitation, the discovery of antibiotics, and the advent of vaccination programs, deaths from infectious diseases subsided and soon gave way to chronic diseases.² Shortly after the turn of the century, diseases of the heart became of the number one cause of death, and in the 1940s, cancer began consistently appearing at number two. Since then, heart disease and cancer have remained firmly entrenched as the number one and two causes …

Thymidine Kinase 1: The Key To Predicting Cancer Recurrence, Rachel Brog, Dr. Kim L. O'Neill

Journal of Undergraduate Research

Currently it is estimated that over 230,000 women will be diagnosed with breast cancer this year in the United States alone. Although treatments are improving, an alarming number of these cases will show cancer recurrence after treatment. A method to consistently predict cancer recurrence is crucial to better treat cancer and improve patient care. Thymidine Kinase 1 (TK1) has been found in abnormally high levels in serum and tissue of cancer patients compared to healthy patients. There is question to why TK1 is present in such high quantities when normally it aids in the DNA salvage pathway during S phase …

Splice Site Predictor, Mark Wadsworth, Dr. Perry G. Ridge

Journal of Undergraduate Research

With the advent of nextgeneration sequencing, one of the unintended consequences is the sheer number of genomic variations requiring interpretation. Mutations in splice sites have been shown to contribute to the development of cancer [1], and dementia [2] among other potentially deadly disorders. Roughly 14 million people are diagnosed with cancer every year [3], and roughly 7 million with dementia [4]. Since these diseases cause an incredible amount of suffering, scientists in all fields are driven to search for ways to identify and treat them. These have been particularly difficult to interpret and have been largely ignored by the bioinformatics …

Flies As Bioindicators Of Grazing Pressures In Mongolia, Rebecca Clement, C. Riley Nelson

Journal of Undergraduate Research

For over fifty thousand years, humans have affected their surrounding environments in a negative way. As the human population continues to increase, concerns about extinction and other human-influenced problems including climate change, habitat degradation, pollution and introduction of invasive species are becoming more and more difficult to ignore (Oreskes, 2004). Mongolia, with its low population density and richly diverse biogeographical landscape, has retained much of the biota that has been lost in surrounding more populous Asian countries. Much of the fauna is relatively unknown. A growing concern in Mongolia is the commercialization of grazing. Although grazing has been an integral …

Discovery And Characterization Of Honey Bee Larvae Gut Microbiota, Christopher Fajardo, John Chaston

Journal of Undergraduate Research

Honey bees are currently plagued by an infection known as American Foulbrood (AFB). This disease is caused by the bacterium Paenibacillus larvae. It is carried by adult Honey bees and becomes an active infection once inside the digestive tract of a Honey bee larvae. Current treatment of this disease is a broad-spectrum antibiotic with negative effects on both the Honey bee and the environment. Research is currently being conducted with the hope to introduce a bacteriophage therapy to the market. This approach will be specific to Paenibacillus larvae without the worries of the current treatments. Our focus is to provide …

Hydrophobic Seed Coating, Karlee Humrich, Dr. Matthew Madsen

Journal of Undergraduate Research

The western rangelands are the wide open spaces of American history. They are increasingly used as a recreational resource by millions of visitors each year. Rangelands produce tangible products such as forage, wildlife habitat, water, minerals, energy, plant and animal gene pools, recreational opportunities, and some wood products. There has been a history of rangeland degradation in the U.S. Because of this degradation, native plant species are not as dominant as they once were. Invasive plant species, such as cheat grass, have integrated into the precious rangeland system. These invasive species out compete and take over native plants. The overall …

Damselflies Of Patagonia: A Phylogeographic Study Utilizing Epic, Taylor King, Seth Bybee

Journal of Undergraduate Research

While working on my original project, “In Situ Hybridization of Opsins in Odonata,” I encountered abundant setbacks that led to refocusing my efforts on a similar project. Patagonian Odonata is one of the most well known insect groups in South America. Within the order Odonata there are two main suborders, encompassing dragonflies and damselflies. It is composed of 36 species in 18 different genera, with 60% of the species and 40% of the genera endemic to the region. We selected two damselfly species, Rhionaeshna variegata and Cyanallagma interruptum to perform the first phylogeographic study of Patagonian Insects. They are the …

The Role Of Pas Kinase And Cbf1 In Cellular Metabolism, Jenny Adele Pattison, Julianne H. Grose

Journal of Undergraduate Research

The most recent National Institute of Health report concludes that 68.8% of American adults are considered to be overweight or obese1. With this trend in obesity, metabolic diseases such as heart disease, stroke, cancer, and diabetes are on the rise. The critical yet basic function of cellular homeostasis is a central component in controlling these diseases. Sensory protein kinases are essential in the phosphorylation of many protein substrates, allowing them to control several metabolic functions and appropriately allocate glucose, maintaining homeostasis in cells. PAS kinase is a sensory protein kinase that is highly conserved and plays a crucial role in …

Proposal Of A New Genus And A New Species Of Chrysomelid Beetle, E. Russell Anderson, Shawn Clark

Journal of Undergraduate Research

The beetle family Chrysomelidae contains many species and genera which are of agricultural importance, which can cause millions of dollars of damage. While studying Triarius, a genus largely overlooked among Chrysomelidae because of its low agricultural impact, we have recognized that Triarius vittipennis (Horn, 1893) differs markedly from other species in several characters that we regard as key. Accordingly, we propose a new genus to accommodate T. vittipennis, as well as a new Mexican species which we are describing and naming now. Additionally, we are describing a second new species, which we place in the genus Triarius.

Bacteriophages As A Biocontrol Agent Of Soft Rot In Potatoes, Trevor Hodson, Don Breakwell

Journal of Undergraduate Research

Pectobacterium carotovorum (Pcc) is a principle causative agent of soft rot in potatoes. It causes losses of up to 60% in potato yields in the USA (Mantsebo et. Al, 2014) and approximately $50-$100 million dollars’ worth of losses yearly in multiple types of crops. Because potatoes grow beneath the soil surface, there is no way of treating the potatoes until they are harvested. Even then, no postharvest methods of controlling this pathogen exist (Wood et. Al, 2013). A spray-able bacteriophage cocktail specific to Pcc could save millions of dollars-worth of crops each year by minimizing soft rot.

The Efficacy Of Tgfβ Inhibition Via Sgi-1252 In The Prevention And Reversal Of Diet-Induced Obesity And Diabetes, Blake Dallon, Benjamin Bikman

Journal of Undergraduate Research

The growing worldwide incidence of obesity and its associated pathologies, like type 2 diabetes, has received much deserved attention. However, despite this attention and substantial research efforts, little meaningful progress has been made in slowing or reversing the growing cost and trends of obesity worldwide.

As of 2008, almost 10% of medical costs in the United States were incurred due to obesity or other obesity-related diseases¹, with a substantial amount of this cost stemming from the dramatically increased risk of diabetes with excess fat mass². According to the US Centers for Disease Control and Prevention, more …

Effects Of Age And Prior Reproduction On The Cost Of Reproduction In Two Species Of Burying Beetles, Ethan Damron, Dane Jo, Mark Belk

Journal of Undergraduate Research

Understanding how much energy an organism allocates to breeding to maximize reproduction over their lifetime, or the cost of reproduction, is a central idea to understanding the variations in patterns of reproduction among living organisms (Creighton et al., 2009). In most studies of cost of reproduction, age is confounded with prior reproductive experience. As individuals age, they tend to reproduce making it difficult to disentangle these two factors when evaluating costs of reproduction. It is not clear whether reproduction early in life costs the same as reproduction later in life and how much of that difference might be due to …

Polymorphisms Of The 5-Httlpr Gene: Associations To Ern And Depression, Curtis Killpack, Michael J. Larson

Journal of Undergraduate Research

Our aim with this study of polymorphisms in the serotonin transporter gene (5-HTTLPR) was to investigate the association between performance monitoring capabilities (i.e., detecting errors in performance using the error-related negativity [ERN] component of the scalp recorded event-related potential [ERP]) against three variations of gene 5-HTTLPR allelic pairs: homozygous short (S/S), heterozygous short-long (S/L), and homozygous long (L/L). The serotonin transporter gene is associated with our ability to cope with stress and regulate serotonin, which affects mood, social behavior, appetite, and sleep (Adam, Doane, Zinbarg, Mineka, Craske, & Griffith, 2010). Previous studies (Barnes, Dean, Nandam, O’Connell, & Bellgrove, 2011) have …