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Articles 31 - 60 of 106
Full-Text Articles in Life Sciences
Using Intrinsic Properties Of Polyaniline To Sense Expression Of The Microrna Let-7, Jared N. Gloria
Using Intrinsic Properties Of Polyaniline To Sense Expression Of The Microrna Let-7, Jared N. Gloria
Honors Theses
MicroRNAs are approximately 22-nucleotide long RNA molecules that function through decay and translational repression of messenger RNA. The microRNA let-7 is found to play a role in maintaining the fate of differentiated cells in humans. Thus, expression level of this microRNA is a reliable biomarker of tumor cell phenotype. However, there are significant limitations in the current profiling techniques of microRNA. The current methods like northern blotting, microarrays, RT-PCR, or using locked nucleic acid (LNA) for in-situ hybridization are either laborious, semi-quantitative, or expensive. In this research we try to address this issue by developing a fast, specific, and inexpensive …
Interlimb Differences In Movement Accuracy In Darkness Vary With Handedness Laterality Scores In Right-Handers, Taylor Amedee
Interlimb Differences In Movement Accuracy In Darkness Vary With Handedness Laterality Scores In Right-Handers, Taylor Amedee
Honors Theses
No abstract provided.
Developing A Deletion Construct Of The Halothiobacillus Neapolitanus Csos1c Gene, Ellyn K. Dunbar
Developing A Deletion Construct Of The Halothiobacillus Neapolitanus Csos1c Gene, Ellyn K. Dunbar
Honors Theses
The purpose of this research was to develop a deletion construct for the chemoautotrophic bacterium Halothiobacillus neapolitanus, which will be used to generate a mutant lacking a carboxysome shell protein gene. The carboxysome is the location of carbon dioxide fixation. The operon that encodes the carboxysome contains three genes for CsoS1 proteins, the major components of the carboxysome shell. The small CsoS1 proteins self-assemble into hexamers with small central pores. The hexamers arrange into the facets of the icosahedral carboxysome shell. The pores are believed to be involved in selective diffusion of materials necessary for carbon dioxide fixation across the …
Sensitivity Of The Vasoactive Range In Determining Aerobic Fitness, Preston L. Bell
Sensitivity Of The Vasoactive Range In Determining Aerobic Fitness, Preston L. Bell
Honors Theses
Previous work has demonstrated a direct relationship between aerobic fitness and indices of vasodilatory function (i.e., Flow-mediated Dilation; FMD). Importantly, recent evidence suggests that vasoconstrictor function to reductions in blood flow (i.e., Low-Flow Mediated Constriction; L-FMC), as well as during sympatho-excitation (i.e., Cold Pressor Test), may compliment the FMD measure, thus, providing an overall range of vascular responsiveness. The purpose of this thesis project was to test the hypothesis that vasoactive range indices (peak vasodilation + nadir vasoconstriction) are sensitive to aerobic fitness levels in healthy young men. Fourteen males (age: 22±4 yrs) were recruited, and divided evenly into a …
Painless Hematophagy: The Functional Role Of Novel Tick Metalloproteases In Pain Suppression, Joseph W. Jelinski
Painless Hematophagy: The Functional Role Of Novel Tick Metalloproteases In Pain Suppression, Joseph W. Jelinski
Honors Theses
Ticks secrete a plethora of pharmacologically active molecules in their saliva while feeding. These allow the tick to feed upon a host over prolonged periods of time in an itch free and painless attachment. The exact mechanism of pain suppression by the tick has barely been investigated. In this study, two angiotensin converting enzymes (ACEs), members of the metalloprotease family, are identified as potentially responsible for the degradation of pro-inflammatory peptides. It is hypothesized that these tick ACEs block the function of bradykinin through degradation of the peptide, contributing to the tick’s ability to conduct a pain and itch-free host …
Awareness Of Biological Mechanisms Of Artificial Sweeteners And The Effects Of Increased Awareness Via Video Media On Intent To Change Dietary Habits In Traditional College-Age Students, Cassie A. Mahler
Honors Theses
In the past, artificial sweeteners have been touted as weight loss solutions because they theoretically do not interact with the body’s metabolism. However, new research is being done to test this theory, but what is the public awareness of these compounds’ interactions with the body? The purpose of this study is threefold: to assess the current awareness of the biological mechanisms of artificial sweeteners in traditional college-age individuals; to assess the effectiveness of video education in increasing this awareness; and to assess how the increase of this awareness might lead to intentions to change dietary habits. The study consisted of …
Vascular Flora Of The Possum Walk Trail At The Infinity Science Center, Hancock County, Mississippi, Hanna M. Miller
Vascular Flora Of The Possum Walk Trail At The Infinity Science Center, Hancock County, Mississippi, Hanna M. Miller
Honors Theses
The North American Coastal Plain contains some of the highest plant diversity in the temperate world. However, most of the region has remained unstudied, resulting in a lack of knowledge about the unique plant communities present there. This flora catalogues the vascular plant species along the Possum Walk Trail on the southeast corner of the Infinity Science Center in Hancock County, Mississippi. Additionally, interpretive signs have been developed to display information about engaging species and phenomena to increase public interest in native plant life and involve them conservation efforts. The site was surveyed from spring of 2015 to spring of …
The Uas-Gal4 System In D. Melanogaster: An Insight Into The Influence Of Micrornas On The Developmental Pathways Of The Wing, Emily R. Wilson
The Uas-Gal4 System In D. Melanogaster: An Insight Into The Influence Of Micrornas On The Developmental Pathways Of The Wing, Emily R. Wilson
Honors Theses
By examining genetic pathways in D. melanogaster, a better understanding of the homologous regulatory mechanisms in humans can be utilized to further enhance knowledge of the roles of microRNA within development. This study utilizes the UAS-Gal4 system in order to produce a mutant phenotype capable of being visually studied and analyzed, focusing on the developmental pathway of the wing in D. melanogaster. Dissections of the wandering third instar larvae yielded wing disc tissue expressing the downregulation of loquacious and CG17386.
The Transfer Of Commensal And Transient Bacteria Into Deep Tissue Using Dry Needling Needles Through Agar Media Designed To Mimic The Elasticity Of Human Skin, Denisse N. Breton Guerrero
The Transfer Of Commensal And Transient Bacteria Into Deep Tissue Using Dry Needling Needles Through Agar Media Designed To Mimic The Elasticity Of Human Skin, Denisse N. Breton Guerrero
Honors Theses
Needle insertion into skin for the purpose of alleviating muscle pain began with the emergence of traditional Chinese medicinal (TCM) acupuncture. “Dry needling”, a recent alternative to acupuncture, involves inserting a monofilament needle down into the deep tissue of a patient’s skin in order to reach the affected muscle. Despite its effectiveness and low risk of infection, dry needling remains questionable with respect to its safety and the lack of research to confirm the low risk of bacterial transmission via the dry needling needles. In this microbiological study, highly concentrated Tryptic Soy Agar was utilized to simulate the elasticity of …
Bioaccumulation Of Iron In Gambusia Affinis In Lemna Minor Mediated Surroundings, Canaan Sutton
Bioaccumulation Of Iron In Gambusia Affinis In Lemna Minor Mediated Surroundings, Canaan Sutton
Honors Theses
No abstract provided.
Effects Of Creatine, Leucine, And Ethanol On Glial Cell Line-Derived Neurotrophic Factor In C2c12 Skeletal Muscle Cells, Daniel Waters
Effects Of Creatine, Leucine, And Ethanol On Glial Cell Line-Derived Neurotrophic Factor In C2c12 Skeletal Muscle Cells, Daniel Waters
Honors Theses
Glial Cell Line-Derived Neurotrophic Factor (GDNF) is a protein expressed throughout the mammalian nervous system that serves several protective functions. Originally discovered in the brain, where it supports the survival of dopaminergic neurons, GDNF has since been shown to be expressed in skeletal muscle and is vital for the maintenance of motor neurons. The relationship between muscle hypertrophy, and GDNF is not well understood. The purpose of this study was to examine the relationship between hypertrophy and GDNF by inducing hypertrophy in C2C12 skeletal muscle cells using leucine and creatine, inhibiting hypertrophy using ethanol and measuring GDNF content. As measured …
Bacteriophages That Can Lyse Antibiotic Resistant Bacteria, Michael Monaco
Bacteriophages That Can Lyse Antibiotic Resistant Bacteria, Michael Monaco
Honors Theses
A Klebsiella pneumoniae bacteriophage was isolated from waste water and purified over multiple rounds of plaque removals and serial titrations. This bacteriophage was imaged in a transmission electron microscope and then tested with selected antibiotics for effectiveness in reducing optical density of K. pneumoniae colonies. The phage was shown to have an increased effectiveness in reducing the optical density when used together with an antibiotic that the bacteria was susceptible to.
"Patterns In The Abundance Of Benthic Algae In Streams", Courtney Wright
"Patterns In The Abundance Of Benthic Algae In Streams", Courtney Wright
Honors Theses
Benthic, microscopic algae are the main drivers of primary productivity in streams. The relationship between the abundance and productivity of benthic algae in streams and lakes and the availability of limiting nutrients, especially soluble reactive phosphorus (SRP), has been extensively studied (reviewed in Allan and Castillo 2007, Larned 2010). However, nearly all of this research has been conducted on the periphyton assemblage found on hard substrates (e.g., stones, wood), while very little work has addressed the factors affecting the abundance of epipelic algae (i.e., the algae associated with depositional (soft) sediments such as sands and silts). One of the few …
A Guided Inquiry To The Bromination Of Alkenes: An Effort To Foster Student Understanding Of Thin Layer Chromatography In The Organic Chemistry Laboratory, Casey Wright
Honors Theses
Many of the chemistry laboratories taught at Western Michigan University are “cookbook” labs, meaning that students are given an instruction manual with a prescribed set of instructions and a predicted outcome. Therein we present an introductory organic chemistry laboratory experiment to reinforce the student understanding of Thin Layer Chromatography which utilizes a guided inquiry format as part of an effort to move toward discovery based learning in our organic chemistry curriculum. Educational research was performed on classroom artifacts collected from the laboratory. We were able to gain an understanding of the struggles students face in applying general chemistry content knowledge …
Discriminative Stimulus Properties Of 3.0 Mg/Kg Mephedrone In Rats, Nathyn Thompson
Discriminative Stimulus Properties Of 3.0 Mg/Kg Mephedrone In Rats, Nathyn Thompson
Honors Theses
Consumption of a prominent synthetic cathinone known as mephedrone (4-methylmethcathinone) has become a popular alternative to club drugs such as ecstasy, cocaine, and methamphetamine within the past decade. The pharmacological mechanisms that contribute to its subjective effects have yet to be fully characterized and are thus warranted for investigation. The present study employed drug discrimination methods to train eight male Sprague-Dawley rats to discriminate injections of 3.0 mg/kg mephedrone from saline. Various doses of mephedrone, 3,4-methylenedioxypyrovalerone (MDPV), d-amphetamine (AMPH), cocaine, (+)-methamphetamine (METH), and 3,4-methylenedioxy-methamphetamine (MDMA) were investigated for their ability to substitute for the stimulus cues of the training …
Chemosensory Signals From Gravid Females Elicit Behavioral Response From Dominant Male Astatotilapia Burtoni, Cristopher T. Mcvicker
Chemosensory Signals From Gravid Females Elicit Behavioral Response From Dominant Male Astatotilapia Burtoni, Cristopher T. Mcvicker
Honors Theses
No abstract provided.
Development Of A Transposon-Based Vector To Express Large Quantities Of Factor Viii, Allison Mikes
Development Of A Transposon-Based Vector To Express Large Quantities Of Factor Viii, Allison Mikes
Honors Theses
No abstract provided.
Zein Nanoparticle Interaction With Soybean Plants, Kurt Ristroph
Zein Nanoparticle Interaction With Soybean Plants, Kurt Ristroph
Honors Theses
No abstract provided.
Analysis Of Western Lowland Gorilla (Gorilla Gorilla Gorilla) Specific Alu Repeats, Thomas Beckstrom
Analysis Of Western Lowland Gorilla (Gorilla Gorilla Gorilla) Specific Alu Repeats, Thomas Beckstrom
Honors Theses
No abstract provided.
The Effects Of Mitochondrial Dna On The Fitness Of The Marine Crustacean Tigripus Californicus, Ellen Foster
The Effects Of Mitochondrial Dna On The Fitness Of The Marine Crustacean Tigripus Californicus, Ellen Foster
Honors Theses
No abstract provided.
Failure To Achieve Recommended Omega-3 Dha Intake During Pregnancy: Nutrition Education Needed, Carly Thaxton
Failure To Achieve Recommended Omega-3 Dha Intake During Pregnancy: Nutrition Education Needed, Carly Thaxton
Honors Theses
No abstract provided.
One Signal, Two Behaviors: Odor Discrimination In Unmated Versus Mated Female Green Bottle Flies, Lucilia Sericata, George Iannantuono
One Signal, Two Behaviors: Odor Discrimination In Unmated Versus Mated Female Green Bottle Flies, Lucilia Sericata, George Iannantuono
Honors Theses
The green bottle fly, Lucilia sericata, is of critical importance in the field of forensic entomology since it is one of the first insects to arrive at a freshly deceased carcass. These flies use a highly tuned and selective olfactory system to identify and locate the carcass; response to the decomposition odor usually occurs within minutes. Male flies use the carcass for a small protein meal and for locating females while females use the carcass for feeding (unmated) and egg-laying (mated). This divergence in behavior to a common odor cue is the subject of this thesis proposal: do unmated …
Effects Of Combination Ice And Compression Socks On Resting Calf Blood Flow In Trained Male Athletes, Michael John Hudock Jr.
Effects Of Combination Ice And Compression Socks On Resting Calf Blood Flow In Trained Male Athletes, Michael John Hudock Jr.
Honors Theses
Compression socks are common tools that are utilized in the realm of athletics. The purpose of the compression is to increase blood flow to the lower extremities, thereby increasing oxygen and energy sources needed for increased skeletal muscle function and/or recovery. Recently, a product has been marketed that combines the compression element with cryotherapy, the goal being increased blood flow to the lower extremities while reducing inflammation post-workout to quicken recovery. However, to our knowledge, direct measures of blood flow using this type of product have not been performed. Thus, this study looks at the effects of compression with and …
Distribution Of Shell Formation Proteins In Oyster Hemolymph, Hemocytes, And Mantle Tissue, Donald Joseph Kleppel
Distribution Of Shell Formation Proteins In Oyster Hemolymph, Hemocytes, And Mantle Tissue, Donald Joseph Kleppel
Honors Theses
The occurrence and composition of L,3-4-dihydroxyphenylalanine-containing proteins (L-DOPA proteins) that participate in oyster shell formation has not been fully determined. It is known that the oyster mantle tissue is primarily responsible for shell formation and recent research has demonstrated the involvement of the hemolymph (blood) and hemocytes (blood cells). L-DOPA proteins are known to aid in the cross linking of shell formation proteins, in turn creating the insoluble organic matrix formed to produce the organic component of the shell. Using the biomarker amino acid L-DOPA, this research focuses on determining the localization of these shell formation proteins in hemocytes, hemolymph, …
Drosophila Models To Investigate The Role Of Regulation Of Cell Death In Development And Cancer, Anam Hussain
Drosophila Models To Investigate The Role Of Regulation Of Cell Death In Development And Cancer, Anam Hussain
Honors Theses
Cell death is of key importance in maintaining health and normal development. In cancer, cell death is improperly controlled provoking uncontrolled proliferation of cells, which results in severe harm to the body. The Hippo pathway is an identified pathway in Drosophila melanogaster that is involved with regulating the different mechanisms of survival and proliferation within the cells. It is known to interact with a gene known as Dronc that is a key participant in the cell death pathway of apoptosis. Previous work has shown how the loss of certain caspases, which are cysteine proteases, is linked with cell survival. My …
Biodiversity, Ecosystem Function And The Pollination Ecology Of Urban Gardens In Dayton, Ohio, Angela Nicole Giaquinto
Biodiversity, Ecosystem Function And The Pollination Ecology Of Urban Gardens In Dayton, Ohio, Angela Nicole Giaquinto
Honors Theses
Urban agriculture is an ancient concept that is gaining momentum in the United States in connection with urban renewal projects and efforts to mediate urban food deserts. These agriculture efforts represent a potential habitat for pollinator communities within the urban hardscape. Pollinator communities are threatened by urban landscape transformation, and bee species, in particular, are in a state of global decline due to various stressors. Urban agriculture has the potential to sustain both human and ecological communities, and the pollinators that visit gardens may provide pollination to plant species in the surrounding area. We investigated the influence of urban land …
Finding A Correlation Between Zooplankton Abundance And The Aggregation Of Abudefduf Saxatilis (Sergeant Major Damselfish) Beneath Boats, William Duritsch
Finding A Correlation Between Zooplankton Abundance And The Aggregation Of Abudefduf Saxatilis (Sergeant Major Damselfish) Beneath Boats, William Duritsch
Honors Theses
Abudefduf saxatilis, or sergeant major damselfish, are a common reef fish in the Caribbean and western Atlantic that form large feeding aggregations. Abudefduf saxatilis are primarily planktivorous, with zooplankton making up over 50% of their diet. Zooplankton are known to have diel movements to avoid predation, which have been shown to be triggered by the presence of ultra violet radiation. Beneath boats along the coast of Bonaire, aggregations of A. saxatilis have been observed, but why they prefer these areas over the open water column had not previously been examined. The abundance of zooplankton was estimated beneath boats as …
The Effects Of Low Dam Removal And Kayak Run Installation On The Biodiversity Of Fish And Macroinvertebrates In The Great Miami River In Downtown Dayton, Ohio, Sarah Anne Stalder
The Effects Of Low Dam Removal And Kayak Run Installation On The Biodiversity Of Fish And Macroinvertebrates In The Great Miami River In Downtown Dayton, Ohio, Sarah Anne Stalder
Honors Theses
Five Rivers Metroparks and the Miami Conservancy District have made plans to remove the upper portion of the Monument Avenue low-head dam in downtown Dayton due to the hazard it poses for recreation on the river and its negative impact on water quality and biodiversity. The plans also include modifying the dam with “kayak drops” and the construction of a second kayak drop slightly upstream of the location of the current low dam to enhance recreational opportunities on the river. Typically, low dam removal alter the flow and depth of a river, especially above the dam in the impounded area …
Evolution Of Alu Element Subfamilies In Papio Baboons, Jon Hunter Strohmeyer
Evolution Of Alu Element Subfamilies In Papio Baboons, Jon Hunter Strohmeyer
Honors Theses
No abstract provided.
15-Lipoxygenase-2 Expression And Activity In Hek 293 Cells, Kirstie Rae Schieffler
15-Lipoxygenase-2 Expression And Activity In Hek 293 Cells, Kirstie Rae Schieffler
Honors Theses
No abstract provided.