Life Sciences Commons^™

Grass Finishing Systems For Lambs, Erin Leigh Smyth

Graduate Theses and Dissertations

There is strong interest in sustainably produced meat. Grass-fed lamb could fulfill this market by reducing off-farm inputs. The objective was to examine the effect of grass-finishing or minimal supplementation on performance of lambs in the southeastern US. Katahdin lambs born in October 2013 and 2014 and February 2014 were weaned in January 2014/2015 and May 2014, respectively. Lambs were blocked by gender (fall; winter included only ram lambs) and randomly assigned to receive no (NON) or grain co-product supplement (SUP; 15% CP) at 0.5% of BW/d. Lambs were rotationally grazed on predominantly grass. Body weight, fecal egg counts (FEC), …

Novel Biomarkers For Calcium And Phosphorus Metabolism In Breeder Hens And Broilers, Andrew Dick Magnuson

Graduate Theses and Dissertations

Broiler breeder hens are subject to the dual expectation to not only maintain a high production of eggs for an extended time period, but to produce eggs which can support the life of chicks which will be used for either meat production or as parent stock. Egg fertility and hatchability are heavily influenced by the thickness of the egg shell, the mineral calcium carbonate shell of the egg necessary for protecting the embryo growing inside. Many factors affect egg shell quality including age of the hen, diet, environmental conditions, genetic strain, stress, disease, and nutrition. Laying hens will mobilize calcium …

Monochamus Titillator Oviposition And Intraspecific Competition In Shortleaf Pine Bolts, Ryan Patrick Rastok

Graduate Theses and Dissertations

Monochamus titillator (Coleoptera: Cerambycidae) feed on trees in the family Pinaceae as adults and larvae. Adults feed on healthy twigs and branches and oviposit on stressed, dead, or dying trees. Prior to oviposition, females construct pits on the bark surface using their mandibles. The number of eggs laid beneath bark, where oviposition pits have been constructed, varies greatly. Developmental time and adult body size also vary greatly. I investigated oviposition and intraspecific competition with the goal of determining the degree to which various insect densities and oviposition times impact the following: 1) oviposition pit construction; 2) egg deposition; 3) emergence, …

Temperature In The Cotton (Gossypium Hirsutum L.) Canopy And Effects On Cotton Leaf And Boll Growth, Maria Soledad Berlangieri Costa

Graduate Theses and Dissertations

Cotton development and yield are negatively influenced by extremes temperatures, mainly during reproductive stage. Ambient air temperature is used to evaluate temperature stress effect on yield under field conditions; however, there is evidence that actual temperatures in the canopy where bolls develop are different. In terms of cotton responses, there is limited research about the effects of elevated day and night temperatures upon boll carbohydrate content, size, and boll respiration. Field and growth chamber experiments were performed during 2014-2015 using the cotton (Gossypium hirsutum L.) cultivar DP0912B2RF. Field studies for determining temperature gradients through the canopy consisted of two planting …

The Comparative Effects Of Hot Yoga And Thermoneutral Yoga On Flexibility, Heart Rate, Sweat Rate, And Mood, Hannah Kirkland Campbell

Graduate Theses and Dissertations

Yoga is an ancient Indian philosophy, described as a therapeutic intervention and health maintenance practice that unites the mind and body to aid healing through the combination of physical postures, breathing techniques, and meditation. There is ample research regarding yoga practiced in an environment that is thermoneutral, or in an environment that does not alter the metabolic heat production or evaporative heat loss of people. However, minimal research exists on the increasingly popular form of yoga known as hot yoga, or yoga practiced in an environment that is often humidified and 95° F or warmer. This exploratory study compared the …

Water Consumption Behavior In Broilers, David Harold Mccreery

Graduate Theses and Dissertations

A series of trials were conducted to analyze broiler water consumption behaviors under commercial conditions. The first trial was to quantify the number of broilers drinking at times of peak water demand. Birds were evaluated at time intervals of 5, 10, 15, 30, 45, and 60 minutes from the start of the light period to establish the percentage birds having consumed water by that point in time. Significant differences were found by bird age in the 5, 10, and 15 minute intervals, however there were no significant differences in the percentage of birds having consumed water in the 30, 45, …

A Cradle To Farm Gate Life Cycle Analysis Of Land Use In U.S. Pork Production, William Benjamin Putman

Graduate Theses and Dissertations

The goal of this study was to conduct a detailed Life Cycle Assessment (LCA) of the U.S. live swine production supply chain to quantify land use requirements and to assess the impact associated with various ration compositions. The functional unit was defined as one kilogram (2.2 pounds) of live swine at the farm gate, ready for transport to the abattoir. This assessment focused on the three highest producing USDA regions, which encompassed the Midwest (Regions 5 and 7) and the Southeast (Region 4), representing 86% of U.S. market hog production.

First, a literature review was conducted to summarize the most …

The Longitudinal Effects Of Beta-Alanine On Punch Power, Punch Frequency, And Fatigue In Female Amateur Fighters, Rodger Wayne Stewart

Graduate Theses and Dissertations

Beta-alanine is a non-essential amino acid typically obtained in the diet via high-protein foods (beef, chicken, pork, and fish) and produced in the confines of the liver. BA is a precursor to the cytoplasmic dipeptide carnosine (beta-alanylhistidine). Carnosine is found in high concentrations in human skeletal muscle where it plays a key role in intracellular pH buffering. Research indicates BA supplementation increased punch power, punch frequency, and lactate concentrations in male amateur fighters after 28-day supplementation. There are currently no studies evaluating the effects of BA on boxing performance variables in women. Therefore, the purpose of this study was to …

Life Cycle Assessment Of Alternative Swine Management Practices, Prathamesh Avadhut Bandekar

Graduate Theses and Dissertations

Life Cycle Assessments (LCAs) are quantitative analyses of complex systems for evaluation of impacts and risk associated with management decisions. LCAs can be effective tools for determining comparative advantages of management strategies across specific impact concern. In this study, life cycle assessments of pork production management alternatives was performed. The alternative management practices included in this study were production of entire males (boars), use of pens for gestation housing, immunocastration, production without growth promoting antimicrobials, production without growth promoting and preventive antimicrobials, and production without ractopamine. These LCAs evaluated the impact of each management strategy on greenhouse gas emission (GHG), …

Effects Of Soybean Pectin On Blood Glucose And Insulin Responses In Healthy Men, Melissa Ann Jones

Graduate Theses and Dissertations

The goal of this study was to examine the effects of soy pectin on postprandial plasma glucose and insulin responses in healthy adult males␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣ intestinal microbiota to determine if differences in weight or body mass index would be linked to differences in microbiota populations. Pectin is a soluble fiber with demonstrated health benefits such as the ability to lower blood glucose and cholesterol, increase satiety leading to lower caloric intake, improve insulin resistance and lower inflammation. These benefits suggest that pectin may help in prevention and treatment of type II diabetes. Soy pectin is extracted from hulls, a waste product …

Is Chir Pine Displacing Banj Oak In The Central Himalaya? Socioeconomic Implications For Local People And The Conservation Of Oak Forest Biodiversity, Ankush Nautiyal

Graduate Theses and Dissertations

Various studies have suggested that chir pine (Pinus roxburghii) is replacing banj oak (Quercus leucotrichophora) in the Central Himalaya. Five sites with three different types of forests (banj oak, chir pine and mixed oak-pine) were sampled to compare the diversity of their vegetation and to assess the impact of this ongoing conversion on biodiversity. Soil samples collected from oak and pine forests were analyzed and compared. In addition, dendrochronology was used to obtain age estimates of chir pine and to understand the growth response of this species to precipitation. Also, samples of ectomycorrhizal fungi were collected in the form of …

Numerical Simulation Of Terahertz Wave Interaction With Breast Cancer Tumor Tissue Sections, Abayomi Omotola Omolewu

Graduate Theses and Dissertations

This thesis presents numerical simulation of terahertz (THz) wave interaction with breast cancer tumor tissue sections. The obtained results are expressed in THz images of heterogeneous material that mimics the excised breast cancer tissue sections. The finite-element software package ANSYS High Frequency Structural Simulator (HFSS) was used in this work. HFSS is a full wave frequency domain three-dimensional (3D) electromagnetic simulation package. In this work, four breast cancer tissue models based on pathology images were simulated and images of the models were obtained at 1 THz. An incident Gaussian beam was raster scanned over tissue model configurations and the reflected …

Long-Term Effects Of Alternative Residue Management Practices On Near-Surface Soil Properties And Soybean Production In A Wheat-Soybean, Double-Crop System In Eastern Arkansas, Christopher Ryan Norman

Graduate Theses and Dissertations

Adoption of management practices that maintain or increase soil organic matter (SOM), which contains 58% carbon (C) on average, may help to mitigate climate change by sequestering atmospheric C. Therefore, the main objective of this study was to determine the long-term trends in SOM, soil C and nitrogen (N), bulk density, various soil chemical properties (i.e., pH, electrical conductivity [EC], and Mehlich-3-extractable nutrients) in the top 10 cm, and soybean yield as affected by residue burning (burning and non-burning), tillage (conventional and no-tillage), irrigation (irrigated and non-irrigated), and N-fertilization/residue level (high and low) in a wheat (Triticum aestivum L.)-soybean [Glycine …

Shiga Toxin-Producing Escherichia Coli (Stec) Detection Strategies With Formalin-Fixed Stec Cells, Christopher Adam Baker

Graduate Theses and Dissertations

Certain pathogenic Escherichia coli known as Shiga toxin (Stx)-producing Escherichia coli (STEC) are commensals in cattle, and typically cause bloody diarrhea in humans once the Stx toxin is secreted in invaded intestinal epithelial cells. Infections with STEC cells can lead to hemolytic uremic syndrome, which is commonly associated with kidney failure. Several STEC serogroups have been declared adulterants in raw, non-intact ground meat, and future regulations could potentially lead to a higher number of STEC serogroup detection strategies for these pathogenic microorganisms. Microbiological research laboratories may benefit from formalin-fixed STEC cells for periodic (daily, weekly, monthly, among others) instrument validation/calibration …

Using Peptoids To Build Robust, Efficient Microarray Systems, Dhaval Sunil Shah

Graduate Theses and Dissertations

Recent studies have shown microarrays to be indispensable for various biological applications, allowing for high-throughput processing and screening of biological samples such as RNA, DNA, proteins and peptides using a small sample volume (< 1 µL). Peptoids (poly-N-substituted glycine oligomers) can be used as a substitute for antibodies as capture molecules, as well as coatings for slides in antibody microarrays. The ease of synthesis of peptoids, high customizability with desired bioactivity, and speed of synthesis allows us to build a diagnostic system with a large dynamic range that can detect biomolecules from a minimal sample size. In this study, peptoid-based antibody mimics are designed to have both structural and functional features similar to those of antibodies, including a stable constant region (scaffolding) and a variable region for protein recognition. Peptoids previously screened via combinatorial library synthesis to be specific to bind Mdm-2 (mouse double minute 2 homolog) and GST (gluthathione S-transferase), have been synthesized. The protein recognition peptoids have been conjugated to PEG (polyethylene glycol) molecules with modified end groups; an amine group on one end that allows for immobilization and orientation on the slide, and an azide group on the other end that will allow for attachment to the peptoid through “click chemistry”. The number of capture molecules printed on the slides can be increased by making the available surface area of the slide larger via coating with microspheres. We have determined that partially water soluble peptoids that are also helical, can self-assemble into microspheres. Sequences have been developed that can consistently produce uniform microsphere coatings on slides that increase the overall surface area. A high surface area corresponds to a higher number of binding sites, and therefore a more sensitive system. The work done has shown that slides may be successfully coated in order to potentially improve the detection system.

The Longitudinal Effects Of Beta-Alanine Supplementation On Isometric Strength, Time To Exhaustion, And Lower-Body Isometric Torque In Female Masters Athlete Cyclists, Jordan Mckenzie Glenn

Graduate Theses and Dissertations

Within the population of aging individuals exists a subset of competitive seniors or masters athletes (MA). As masters-level competition increases in popularity, MA must find methods to enhance individual athletic performance. Beta-alanine (BA) is an amino acid used to enhance physical capability based on ability increase intramuscular carnosine concentrations. Older adults and females have naturally lower carnosine levels compared to age- and gender-matched counterparts and may experience enhanced benefits from BA supplementation. Purpose: Therefore, the purpose of this investigation was to examine the longitudinal effects of BA supplementation on isometric handgrip strength (HG), time to exhaustion (TTE), total work completed …

Association Mapping For Elemental Concentration Resulting From Vegetative Stage Soil Waterlogging Stress In Wheat (Triticum Aestivum L.), Maria Nelly Arguello-Blanco

Graduate Theses and Dissertations

Soil waterlogging (WL) affects wheat production worldwide, including the southern of the U.S. Little is known about the genetics of tolerance mechanisms to WL, particularly of potentially toxic elements such as aluminum, iron and sodium. The objectives of this study were to use association mapping (AM) to identify SNP associated with the EC in the vegetative tissue of a set of 240 diverse soft winter wheat genotypes (AM panel) grown under field WL and to determine the impact of WL on wheat yield (GY) and yield components in a set 28 adapted cultivars (YT)

YT study showed reductions in GY …

Phenology And Diversity Of Arthropod Communities In Leaf Litter, Fredericka Hamilton

Graduate Theses and Dissertations

Temperate deciduous forests produce a layer of leaf litter on the forest floor annually. This layer is dynamic, and both the composition and depth change throughout the year. The leaf litter layer is an important habitat to many arthropods since they utilize it for food, shelter from adverse environmental conditions, and protection from predators.

Two commonly encountered and diverse taxa found in the litter layer are the ground beetles (Coleoptera: Carabidae) and the ground spiders (Araneae: Gnaphosidae). Carabidae and Gnaphosidae were collected and identified on a monthly basis from April 2014 to March 2015 from the Ozark Mountains of Arkansas …

Designing Fret Assays To Study Electrostatic Interactions Pertaining To The Binding Of Intrinsically Disordered Proteins, Ashley Ann Howard

Graduate Theses and Dissertations

Fibroblast growth factor receptor plays a major role in several biological processes. Without FGFR, a human cannot live. FGFR is involved in cell differentiation and wound healing. Of course, if FGFR signaling becomes unregulated, it causes severe distress in the body. Several cancers are contributed to high signaling levels, as well as developmental conditions like rickets and Kallmann’s syndrome. FGFR is thought to undergo an auto-inhibition (or self-regulatory) process in order to try to facilitate regulation. The exact method of this inhibition is currently unknown, but is proposed to involve the unstructured acid box region of FGFR. We developed a …

Biofilm-Related Materials As Total N-Nitrosamine (Tono) Precursors And Hydroxylamine-Based Interferences In Tono And N-Nitrosodimethylamine (Ndma) Measurements, David Allen Meints

Graduate Theses and Dissertations

In this study, a chemiluminescence-based total N-nitrosamine (TONO) assay was adapted to include a solid-phase extraction (SPE) step to assess the role of biologically derived materials as N-nitrosamine precursors. Methanol was determined to be a suitable solvent for the SPE-TONO assay, with N-nitrosodimethylamine (NDMA) losses estimated to be 30% by SPE alone and an additional 15% from concentration of the methanol extracts by N2-gas blowdown. Three biofilm-derived materials - poly-N-acetylglucosamine (PNAG), Pseudomonas aeruginosa, and tryptophan - were individually chloraminated and dose-response relationships were observed with the SPE-TONO assay, indicating biofilm are potential N-nitrosamine precursors. The role of hydroxylamine - a …

Effects Of Mild Hypohydration And Hyperthermia On Cognition And Mood In Obese And Non-Obese Females, Jenna Marie Burchfield

Graduate Theses and Dissertations

INTRODUCTION: Information regarding effects of hypohydration (HY) and hyperthermia (HT) on cognition, mood and development of symptoms is conflicting since the two conditions often confound each other. Further, although obese individuals may have physiological impairments during heat stress, whether psychological impairments occur with HY and HT is unknown. PURPOSE: To assess the independent and combined effects of mild HY and HT on cognition, mood, and the development of symptoms in obese and non-obese females. METHODS: Twenty-two healthy females (11 non-obese, 22±2y, 61±6kg, 25±4% body fat; 11 obese, 22±2y, 80±18kg, 44±5% body fat) volunteered in two randomized, repeated-measures trials, involving passive …

Effects Of Crystalline Amino Acid Supplementation Of Reduced Crude Protein (Rcp) Diet On Net Energy Basis On Growing-Finishing Swine, Daniel Cook

Graduate Theses and Dissertations

Barrows and gilts (n=210) were used to test the effects of crystalline amino acid (AA) supplementation of reduced crude protein (RCP) diets formulated on a net energy basis on quality characteristics of the LM from growing-finishing pigs. Pigs were blocked by weight, and pens (3 barrows and 3 gilts/pen) within each block were assigned randomly to either corn-soybean meal diets (C) or 1 of 3 RCP diets and added crystalline AA levels for the dietary treatments during each feeding phase. During the last 3-wk feeding phase, 10 ppm of ractopamine were included in all diets. At slaughter and after a …

Local Modulation And Measurement Of Macrophage-Derived Bioactive Proteins From Implanted Biomaterials In Rat, Geetika Bajpai

Graduate Theses and Dissertations

Fibrosis around the implanted medical devices is a severe problem that can plague long-term device reliability. Activation of macrophage phenotype (macrophage polarization) has emerged as a new and possible means for reducing fibrosis in the fields of biomaterials and regenerative medicine. Macrophages are phagocytic cells that respond to microenvironmental cues that direct their phenotype. Macrophage activation has been widely studied in mouse and human in the context of tumor biology, yet little information is available regarding how macrophage activation could be used in a biomaterials context. Further, rats rather than mice are the common subjects in biomaterials experiments. A significant …

Nutrient Management For Growing Dandelion (Taraxacum Officinale L.) In Nutrient Film And Deep Flow Hydroponics, Reetinder Gill

Graduate Theses and Dissertations

This research study was conducted to determine an optimal nutrient strategy for dandelion production in nutrient film technique (NFT) and deep flow technique (DFT) systems of hydroponics. It was achieved by growing dandelion at varying levels of nutrient solution concentration and pH in both NFT and DFT systems. Additionally, an optimal nutrient solution concentration and timing of application for dandelion seedling production was also determined.

Direct Fermentation Of Sweet Potato Starch Into Lactic Acid By Lactobacillus Amylovorus: The Prospect Of An Adaptation Process, Winifred Akoetey

Graduate Theses and Dissertations

Several studies have verified that lactic acid bacteria are capable of directly producing lactic acid from starch. One of these bacteria, L. amylovorus, has been studied using corn, potato, and cassava starches. However, the use of sweet potato starch, a widely available starch, has not been considered. Furthermore, there exists the possibility that the efficiency of lactic acid production can benefit from the systematic exposure of bacteria to increasing concentrations of a particular starch. In this work, fermentation studies were conducted to understand the adaptation of L. amylovorus to rising concentrations of sweet potato starch. L. amylovorus was cultured in …

Electrostatic Atomization Of Antimicrobial Treatments On Ground Beef Processing, Instrumental Color, Sensory Color, Taste And Aroma Characteristics, Jorge Abraham Marcos

Graduate Theses and Dissertations

The general objectives of this research were to evaluate the interaction effects of sixteen different antimicrobial treatments throughout seven days of display on physical, chemical and sensory characteristics of ground beef patties, when compared to an un-treated control. Antimicrobial treatments included: fumaric acid, malic acid, octanoic acid, decanoic acid, sodium propionate, propionic acid solution, potassium lactate/diacetate blend, sodium benzoate, hexanoic acid, pyruvic acid, levulinic acid, lactic acid/ citric acid blend, sodium diacetate, lemon juice and acetic acid. Prior to grinding, beef trimmings (80/20) were electrostatically sprayed with antimicrobial treatment solutions. Ground beef was processed into meat patties and sampled for …

Perceptual Differences In Children Learning To Play Golf With Traditional Or Modified (Scaled) Equipment, Lindsey Dawn Sams

Graduate Theses and Dissertations

The purpose of the present study was to examine perception differences of competence and enjoyment between learners using modified and traditional equipment, and the potential effects these factors could have on intent for future participation. The participants in this study were 123 children (65 girls, 58 boys) aged 7 to 17 years. Through a convenience sampling method, data was obtained through surveys measuring golf participation and self-perceptions concerning perceived competence, enjoyment and intention to continue participation. To avoid an age confound, the dataset was limited to 2nd through 5th grade children (24 traditional/ 75 modified) for secondary analyses (MANOVA and …

A Microhabitat Assessment Of Five Species Of Invasive Plants In The Ozarks And Appalachians, Eric Hearth

Graduate Theses and Dissertations

Invasive species present a threat to native communities and their introduction and expansion can alter community structure and dynamics. Multiple approaches can be employed for invasive species management including prevention and detection. In this study, microhabitat assessments were conducted on colonies of five species of invasive plants, Alliaria petiolata (M. Bieb.) Cavara & Grande, Lespedeza cuneata (Dum. Cours.) G. Don, Lonicera japonica Thunb., Microstegium vimineum (Trin.) A. Camus, and Rosa multiflora Thunb. in the Ozark Plateau and Appalachians. Elevation, soil moisture, soil pH, light ratio, slope, aspect, distance to disturbance, as well as soil nutrient levels were recorded for each …

Understanding The Evolution Of Aggregative Multicellularity: A Molecular Phylogenetic Study Of The Cellular Slime Mold Genera Sorodiplophrys And Pocheina, Alexander Tice

Graduate Theses and Dissertations

Cellular slime molds are amoeboid protists that have a unicellular trophic phase and multicellular dispersal stage formed through the aggregation of individuals in their life cycles. These organisms were once thought to form a monophyletic group in the Mycetozoa. After careful morphological, ultrastructural, and molecular studies, cellular slime molds are now thought to be distantly related organisms that have all converged on the cellular slime mold habit. The following thesis consists of two molecular phylogenetic studies on two named genera of cellular slime mold for which little or no molecular data were publically available. In the first study, gene sequence …

Efficacy Of Beta-Resorcylic Acid To Reduce Campylobacter Jejuni In Pre-Harvest And Post-Harvest Poultry, Basanta Raj Wagle

Graduate Theses and Dissertations

Campylobacteriosis is one of the leading foodborne illnesses in United States, and is associated with the consumption of poultry and poultry products. Reducing Campylobacter in these species will reduce the burden of this disease. Unfortunately, most strategies employed to reduce Campylobacter in poultry have either not been successful or produced inconsistent results. One potential control strategy is the use of β-resorcylic acid (BR), a phytophenolic compound classified by the US FDA as "Everything Added to Food in the United States" (EAF 3045) and is therefore deemed safe for consumption. This compounds has antibacterial activity against Salmonella, however, its efficacy to …