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Articles 931 - 960 of 3160
Full-Text Articles in Life Sciences
Rab39 And Klp98a Are Required For Furrow Formation During Early Drosophila Embryogenesis, Megan R. Millage
Rab39 And Klp98a Are Required For Furrow Formation During Early Drosophila Embryogenesis, Megan R. Millage
Electronic Theses and Dissertations
The formation of a plasma membrane furrow is an essential process during development. Furrow formation is necessary for successful cell division and cytokinesis in addition to the ability to create multicellular tissues. Here, I will explore the role of the Golgi-associated Rab protein Rab39 in furrow formation during early Drosophila embryogenesis. Rab39 is one of eight Rab proteins that has been shown to localize to discrete puncta by live imaging in early Drosophila embryos, but its function and pathway have not been well characterized. In this thesis will I show that Rab39 forms dynamic, tubular structures that colocalize with trans-Golgi …
Sorting Of Cargo Proteins Within The Regulated Secretory Pathway: The Peripheral Membrane Protein Hid-1 As A Sorting And Vesicle Biogenesis Factor, Blake H. Hummer
Sorting Of Cargo Proteins Within The Regulated Secretory Pathway: The Peripheral Membrane Protein Hid-1 As A Sorting And Vesicle Biogenesis Factor, Blake H. Hummer
Electronic Theses and Dissertations
Large dense core vesicles (LDCVs) form at the trans-Golgi network (TGN) and mediate the regulated release of neuropeptides and peptide hormones. Despite their central role to physiology, the mechanisms controlling biogenesis and sorting to LDCVs is not well understood. Optimizing the retention using selective hooks (RUSH) method in neuroendocrine cells, we show it is possible to visualize sorting to the constitutive and regulated secretory pathways in real-time and that the bulk of transmembrane LDCV cargoes do not sort directly onto LDCVs, but exit the TGN into non-regulated vesicles to be incorporated to LDCVs at a later step. Additionally, we characterize …
Climate Change And Seed Security Among Smallholder Farmers In Northern Ghana, Michael Biwalib Madin
Climate Change And Seed Security Among Smallholder Farmers In Northern Ghana, Michael Biwalib Madin
Electronic Theses and Dissertations
Smallholder agriculture is highly susceptible to climate variability and change. According to recent projections by the Intergovernmental Panel on Climate Change, this sensitivity would likely increase in the coming decades, with more erratic rainfall, prolonged dry periods, shorter growing seasons, and seed germination failures. In the African context, the mechanisms through which these ecological stressors would affect seed security are poorly understood. Drawing upon a case study of semi-arid Ghana, this study examines climate change impacts on seed security among smallholder farmers. It adopts a mixed-methods approach with intensive fieldwork in two farming communities. Conceptually, the study uses a political …
Quantification Of Dynamic Epithelial Sheet Architecture In Botryllus Schlosseri Using 2-D & 3-D Image Analysis, Roopa Madhu
Quantification Of Dynamic Epithelial Sheet Architecture In Botryllus Schlosseri Using 2-D & 3-D Image Analysis, Roopa Madhu
Electronic Theses and Dissertations
Epithelial tubules form critical structures in various body tissues; how- ever, since they are difficult to access experimentally, their architecture and dynamics are not well understood. Here we examine the dynamic remodeling of epithelial tubes in vivo using a novel and uniquely accessible model system: the extracorporeal vasculature of Botryllus schlosseri (sea squirt). In Botryllus, massive retraction of blood vessels can be triggered without loss of barrier function, through (i) disrupting collagen crosslinking in the basement membrane using β-aminopropionitrile (BAPN); or (ii) disrupting the integrin pathway through inhibition of focal adhesion kinase (FAK). We performed stereographic projections of 3-dimensional …
Cellular And Organismal Ramifications Of De Novo Purine Synthesis Dysregulation, Randall Craig Mazzarino
Cellular And Organismal Ramifications Of De Novo Purine Synthesis Dysregulation, Randall Craig Mazzarino
Electronic Theses and Dissertations
Purines are a class of nitrogenous bases and are essential small molecules to life. Purines are used within the cell as genetic information carriers, energy currency, signaling molecules, and cofactors for multiple processes. They are formed through de novo and salvage pathways found in cells across the phylogenetic tree. The substrates of enzymes within de novo purine synthesis are known to influence other processes within the cell, such as energy homeostasis. In humans, de novo purine synthesis disorders are rare, with around 100 people identified. These patients exhibit a range of phenotypes, with varying degrees of mental retardation, seizure activity, …
In Situ Analysis Of Line-1 Promoter Activity Using Lacz Transgenic Mice, Partha Sarathi Saha
In Situ Analysis Of Line-1 Promoter Activity Using Lacz Transgenic Mice, Partha Sarathi Saha
Electronic Theses and Dissertations
Apart from an evolutionary role, transposable elements have been implicated in animal development and also in pathophysiology. Non-LTR retrotransposons– LINE-1, Alu and SVA - are responsible for over 120 cases of human genetic diseases as heritable insertions, and are emerging as an important etiological factor for cancer and neurological disorders as somatic mutations. It is estimated that among the total number of 500,000 LINE-1s presents in the human genome, 80-100 LINE-1s remain competent for retrotransposition. Retrotransposition is only possible when LINE-1 is expressed. Because LINE-1 transcription is regulated by its 5’UTR promoter, it is essential to understand the spatiotemporal control …
In-Season Crop Management Effect On Soybean Yield And Grain Quality In Eastern South Dakota, Kelsey Bergman
In-Season Crop Management Effect On Soybean Yield And Grain Quality In Eastern South Dakota, Kelsey Bergman
Electronic Theses and Dissertations
Soybean [Glycine max (L.) Merr.] yield has increased over time by introduction of newer varieties and improved agronomic practices. However, grain protein concentration has been decreasing at the same time. New field studies in 2018 and 2019 investigated inseason crop management practices effect such as use of foliar protection application, fertilizer application, different maturity length or different planting dates on grain yield and grain quality. The studies were established near Brookings, SD and Beresford, SD. In-season treatments were targeting nutrient availability or protecting soybean canopy during the grain filling period. Such treatments included the use of fungicide, insecticide, or supplying …
Mechanistic Studies To Understand The Role Of Human Gut Microbiota In Colonization Resistance And Gut Health, Sudeep Ghimire
Mechanistic Studies To Understand The Role Of Human Gut Microbiota In Colonization Resistance And Gut Health, Sudeep Ghimire
Electronic Theses and Dissertations
Human gut microbiota is comprised of thousands of species which fall into two major categories or “enterotypes” based on dominating bacteria: “Bacteroides” and “Prevotella”. Bacteroides enterotype dominates the microbiota of the western population because of a high protein and fat diet, whereas Prevotella enterotype is dominate in the gut of the eastern population because of a high carbohydrate diet. While most of the microbiota studies focused on the Bacteroides enterotype using both metagenomics and culturomics, Prevotella enterotype is understudied, especially in the field of culturomics. The structure of the Prevotella dominated gut microbiome is revealed by …
Genomic And Culturomic Analysis Of Gut Microbiota Function And Salmonella Enterica Expansion, Gavin Fenske
Genomic And Culturomic Analysis Of Gut Microbiota Function And Salmonella Enterica Expansion, Gavin Fenske
Electronic Theses and Dissertations
Enteric bacteria that are resident in the hindgut of mammals are critical in immune development, digestion, and colonization resistance against pathogens. One of the major pathogens that gut commensals provide resistance against is Salmonella enterica, a major foodborne pathogen capable of infecting almost every warm-blooded animal. Given the interplay between pathogens and commensals in the gut lumen, the gut microbiota of pigs was studied by combining two disparate techniques: shotgun metagenomics and high throughput culturomics. Metagenomics readily identifies major taxa present in samples and can give an estimation to total genetic catalogue from an environment. However, many rare or …
A Natural Antimicrobial From Bacillus Subtilis As A Biosanitizer For Resilient Membrane Biofilms, Pratishtha Verma
A Natural Antimicrobial From Bacillus Subtilis As A Biosanitizer For Resilient Membrane Biofilms, Pratishtha Verma
Electronic Theses and Dissertations
The microbial attachment and colonization on separation membranes lead to biofilm formation. Some selective isolates within the biofilm constitutive microflora acquire resistance and emerge predominant over prolonged use of the membrane. Thus, proving the cleaning and sanitization protocols to be ineffective in adequately removing resilient biofilms. This subsequently leads to selecting microbial resistance within the constitutive microflora to almost all antimicrobial treatments and hence, creates a need to develop novel alternative strategies to control biofilm formation on membrane surfaces. The first research project under this study was designed to understand the microbial interactions and emergence of predominance within biofilm constitutive …
The Role And Molecular Mechanisms Of Wss1 In Preserving Genomic Stability, Daniel Kwesi Sam
The Role And Molecular Mechanisms Of Wss1 In Preserving Genomic Stability, Daniel Kwesi Sam
Electronic Theses and Dissertations
Cells are constantly under threat from both exogenous and endogenous sources of DNA damage. Eukaryotic organisms, however, possess conserved mechanisms that accurately and faithfully respond to DNA damage. The inability to effectively remove DNA lesions can lead to an accumulation of mutations which can compromise cellular viability. The DNA damage response is conserved from bacteria to eukaryotic organisms and have been well characterized, however, how covalently crosslinked proteins are removed from DNA remains enigmatic This thesis provides genetic and biochemical evidence implicating Wss1, a yeast metalloprotease in genome maintenance. We have identified SUMOylation to be an important signal that mediates …
Understanding The Effect Of Single- And Twin-Screw Extrusion Processing Parameters On Physical And Physico-Chemical Properties Of Sprouted Quinoa And Sprouted Proso Millets, Gabriela John Swamy
Understanding The Effect Of Single- And Twin-Screw Extrusion Processing Parameters On Physical And Physico-Chemical Properties Of Sprouted Quinoa And Sprouted Proso Millets, Gabriela John Swamy
Electronic Theses and Dissertations
According to Mordor Intelligence Research, the new compositional research and moisture content extrusion process are helping the growth of the plant protein market. The demand for plant proteins is growing at a fast rate, owing to change in lifestyle, lack of balanced dietary intake, and improved research and development in order to develop new kinds of plant-protein enriched products. It is necessary to identify the right plant protein sources and choose the right processing methodology to create highly digestible foods which can be consumed by infants and elderly as well. In addition, it is important to support local farmers and …
Impact Of Postweaning Management Strategies On Beef Carcass Characteristics And Meat Quality, Christina Elaine Bakker
Impact Of Postweaning Management Strategies On Beef Carcass Characteristics And Meat Quality, Christina Elaine Bakker
Electronic Theses and Dissertations
The overall objective of this dissertation was to determine the impact of selected post weaning management strategies on beef carcass characteristics and meat quality. The specific objectives were 1) determine the effect of a rumen-protected long chain fatty acids fed during finishing on live performance, serum fatty acid profile, carcass traits, and predicted carcass composition, and 2) determine effects of feeding brassica-based cover crops to cattle during backgrounding on live animal performance, carcass characteristics, tenderness, juiciness, and flavor of strip steaks, and case life of ground beef and strip steaks. Results from both objectives indicate post weaning management strategies do …
The Association Between The Comprehensiveness And Strength Of Written Physical Activity Wellness Policies And The Observed School Physical Activity Environment, Maggie Kringen
Electronic Theses and Dissertations
Purpose: To determine if there is a relationship between physical education/activity wellness policy comprehensiveness and strength and the observed physical activity (PA) environment.
Methods: The Wellness School Assessment Tool 2.0 (WellSAT 2.0) was used to evaluate the quality of written wellness policies. The School Physical Activity and Nutrition Environment Tool (SPAN-ET) was used to evaluate the school environment. Pairwise correlations and one-way ANOVAs were used to determine if written wellness policy quality affects the school PA environment. Data are presented as mean ± SD and significance was set at p ≤ 0.05.
Results: One significant relationship was found between WellSAT …
Cold-Water Prey Fish Monitoring And Ecology In Lake Oahe, Nicholas B. Kludt
Cold-Water Prey Fish Monitoring And Ecology In Lake Oahe, Nicholas B. Kludt
Electronic Theses and Dissertations
In Lake Oahe, a large mainstem Missouri river reservoir that spans 375 river kilometers in central North and South Dakota, naturalized Rainbow Smelt are the primary forage species for a regionally important Walleye Sander vitreus fishery. Walleye condition in Lake Oahe is highly correlated to Rainbow Smelt relative abundance through time, the latter species being typified by erratic abundance trends since accidental introduction in 1971. Cisco Coregonus artedii were introduced in the early 1990s to augment the cold-water prey base. We evaluated Lake Oahe cold-water prey base monitoring and dynamics through: 1.) a comparison of two hydroacoustic survey designs, 2.) …
Reverse Genetic Analysis Of Ehd1 For An Association Between Flowering Time And Seed Dormancy In Rice (Oryza Sativa L.), Yeter Karakoc
Reverse Genetic Analysis Of Ehd1 For An Association Between Flowering Time And Seed Dormancy In Rice (Oryza Sativa L.), Yeter Karakoc
Electronic Theses and Dissertations
This research aimed to understand the evolutionary mechanism of seed dormancy and flowering time and to provide basic knowledge for the manipulation of germination features in crop breeding. Flowering time and seed dormancy are two adaptive traits for flowering plants. Seed dormancy (SD) helps regulate the timing of germination to promote the survival of seed-bearing plants in adverse environments. Cereal crops and weedy/wild relatives, such as Asian cultivated rice and weedy rice (Oryza sativa L.), have weak and strong seed dormancy, respectively. Thus, weedy and cultivated rice has been used as a model system to investigate mechanisms of seed dormancy …
Understanding How Agricultural Intensification Impacts Ring-Necked Pheasant Distribution And Survival In Eastern South Dakota, Hilary R. Kauth
Understanding How Agricultural Intensification Impacts Ring-Necked Pheasant Distribution And Survival In Eastern South Dakota, Hilary R. Kauth
Electronic Theses and Dissertations
Historically, pheasants (Phasianus colchicus) have thrived in South Dakota in conjunction with successful land retirement programs or early farming practices through the 1950s, which created interspersions of agriculture and native landscapes that were ideal for pheasants. Recently, the Prairie Pothole ecosystem has undergone rapid agroeconomic expansion, effectively reducing ideal interspersions of native prairie and cropland into agriculturally homogeneous landscapes. Indices of pheasant abundance have suggested persistent population declines since 2008, raising concerns regarding landscape suitability. Our goal was to understand how agriculture intensification impacts pheasant ecology. The objectives were to: 1) estimate overwinter hen probability of survival, resource …
Interaction Of Streptomyces Closely Associated With Bacillus In Nitrogen-Limiting Conditions, Aminya Baniya
Interaction Of Streptomyces Closely Associated With Bacillus In Nitrogen-Limiting Conditions, Aminya Baniya
Electronic Theses and Dissertations
Biological Nitrogen Fixation is the process of conversion of atmospheric dinitrogen into ammonia which is performed by symbiotic as well as free-living diazotrophic bacteria. A previous project to isolate free-living nitrogen fixing bacteria from a natural prairie yielded Streptomyces which grew on nitrogen free medium. Genome sequencing of three isolates revealed the presence of a second 16S rRNA gene sequence aligning with Bacillus, however repeated streaking only yielded Streptomyces colonies. The aim of this research was to characterize the interaction of three Streptomyces isolates that grow under nitrogen-free conditions, and appeared to be associated with Bacillus. The separation of Bacillus …
Distribution, Density, Movement, And Support For Management Of Mountain Sucker, Pantosteus Jordani, In The Black Hills Of South Dakota, Seth J. Fopma
Distribution, Density, Movement, And Support For Management Of Mountain Sucker, Pantosteus Jordani, In The Black Hills Of South Dakota, Seth J. Fopma
Electronic Theses and Dissertations
Mountain Sucker, Pantosteus jordani, is a cold-water species native to the Intermountain West. Fringe populations of Mountain Sucker have experienced declines in recent decades. The population of Mountain Sucker found in the Black Hills of South Dakota represents the eastern fringe of the species’ native range. Recognized as both an indicator of biologic health and as a species of greatest conservation need in South Dakota, recent studies have suggested significant declines in both distribution and abundance. Despite the recent study of Mountain Sucker in the region, increased understanding of Mountain Sucker ecology is needed to effectively manage for the conservation …
Understanding South Dakota Farmers’ Intentions To And Adoption Of Conservation Practices: An Examination Of The Theory Of Planned Behavior, Edem Avemegah
Electronic Theses and Dissertations
Conservation agriculture practice is a sustainable farming method based on three principle: crop diversification, minimal soil disturbance or movement and permanent or semi-permanent of soil cover. Government and stakeholders within the agricultural sector in the United States are promoting conservation farming practices but limited voluntary adoption still exists among producers at the farm level. This research study investigated the factors that influence the adoption of conservation practices among producers in the eastern and central parts of South Dakota (SD). A modified theory of planned behavior (TPB) is used as a framework alongside socioeconomic and demographic indicators to understand farmers’ current …
Biodegradable Cellulose Films As Alternatives To Plastics, Cecilia Wambui Wanjuu
Biodegradable Cellulose Films As Alternatives To Plastics, Cecilia Wambui Wanjuu
Electronic Theses and Dissertations
Plastics are versatile and have desirable packaging characteristics such as flexibility, durability, and affordability. The production of plastics has increased over the years resulting in concerns about their single-usage and disposal. Since plastics are nonbiodegradable, they have been associated with detrimental health impacts due to the leaching chemicals from the landfills and their accumulation in the natural and marine environment. Cellulose obtained from agricultural residues is a viable substitute because of its low density, biocompatibility, biodegradability, low toxicity, and a strong and stiff structure. However, the extraction of cellulose has been less exploited because of its insolubility in water and …
Biodegradable Biomaterials As Suitable Alternatives To Water Treatment Technologies, Most Farzana Yesmin
Biodegradable Biomaterials As Suitable Alternatives To Water Treatment Technologies, Most Farzana Yesmin
Electronic Theses and Dissertations
Nitrates and phosphates are essential nutrients for plants growth. Their excess presence in water, however, could cause eutrophication affecting the water quality and altering the aquatic ecosystem. Algal blooms and the presence of toxins such as microcystin in freshwater bodies are hazardous to humans, animals, and wildlife. Conventional technologies such as ion exchange, distillation, reverse osmosis, and bioreactors aid immensely to remove nutrients; however, they are expensive. Furthermore, building new nitrate and phosphate treatment units, maintenance and post-disposal are costly too. In this regard, there exists an unmet need for alternative processes that are renewable and cost-effective to treat contaminated …
Development Of Methods To Improve Lactose Recovery From Permeate And Drying Characteristics Of Greek Acid Whey, Venkateswarlu Sunkesula
Development Of Methods To Improve Lactose Recovery From Permeate And Drying Characteristics Of Greek Acid Whey, Venkateswarlu Sunkesula
Electronic Theses and Dissertations
Growth of higher protein dairy products and ingredients such as cheese, Greek yogurt, whey protein concentrates, and milk protein concentrates is limited by the amount of co-product generated and the ability to process them into value added ingredients. Finding value for all the co-products will help dairy industry to meet the growing demand for dairy protein products and ingredients. Commercial scale evaluation of new technologies for improving lactose crystallization and drying ability of Greek yogurt acid whey will help dairy industry to better utilize different dairy co-products. Commercially, lactose is manufactured from concentrated permeate by cooling and separating α-lactose monohydrate …
Functional Analysis Of Squamosa-Promoter Binding Protein Like 13 In Controlling Flowering Time, Plant Architecture, Grain Size, And Grain Number In Wheat, Ajay Gupta
Electronic Theses and Dissertations
Higher and stable yield is always a major objective of wheat genetic improvement programs. The SQUAMOSA-promoter binding protein-like (SPL) genes constitute a small family of plant-specific transcription factors with diverse functions in plant development and growth and have great potential in improving yield and other major agronomic traits. However, the functional characterization of the SPL gene family in wheat is far behind other cereal crops such as rice. Using phylogenetic analysis, we identified 56 wheat orthologues of rice SPL genes belonging to 19 homoeologous groups. Among these 19 orthologous TaSPL genes, nine harbor the micro RNA 156 recognition element (MRE) …
Determining The Effects Of Plant Extracts And Saltro Nematicide On Hatching, Mortality And Reproduction Of The Soybean Cyst Nematode (Heterodera Glycines), Bishnu Prasad Dhital
Determining The Effects Of Plant Extracts And Saltro Nematicide On Hatching, Mortality And Reproduction Of The Soybean Cyst Nematode (Heterodera Glycines), Bishnu Prasad Dhital
Electronic Theses and Dissertations
Soybean cyst nematode (SCN) is a microscopic worm that infects and feeds from the soybean roots, hence robbing the plant of nutrients, and entry wounds may facilitate other pathogens to infect the roots. Current SCN management practices include cultural, chemical, and biological methods. Cultural practices such as, crop rotation with non-host and different tillage practices are not efficient due to the persistent nature of SCN in the soil. Chemical nematicides, though effective, can be expensive, and some are not environmentally sound. SCN resistant soybean varieties are mainly derived from one source, PI 88788 and SCN population have already evolved to …
Influence Of Mink Predation On Brown Trout Survival And Size-Structure In Rapid Creek, South Dakota, Austin Galinat
Influence Of Mink Predation On Brown Trout Survival And Size-Structure In Rapid Creek, South Dakota, Austin Galinat
Electronic Theses and Dissertations
I compared movement, home range, habitat selection, and mortality among radio-tagged Brown Trout (Salmo trutta) at an American Mink (Neovison vison)-removal site, improved habitat site, and an undisturbed stream site in Rapid Creek, South Dakota. I observed high, post-surgery (> 26 days) survival rate (90%) of radio tagged Brown Trout. Average gross movement of Brown Trout was greater at the improved habitat site (127 m) compared to the mink removal (31 m) site, while average home range size (i.e., stream distance) among all three sites was not significantly different indicating strong site fidelity (125 to 200 m). Brown Trout movements …
Assembly Of Triticum Boeoticum Ssp. Aegilopoides And Triticum Monoccocum Genomes, Mustafa Aljadi
Assembly Of Triticum Boeoticum Ssp. Aegilopoides And Triticum Monoccocum Genomes, Mustafa Aljadi
Electronic Theses and Dissertations
Wheat is ancient cereal. Wheat has n = 7 chromosomes which is belong to genus Triticum. The group Triticum monococcum sp. aegilopoides(boeoticum) is a diploid wheat (2n = 14). It has a morphology aspect of having narrow, flat spike which usually shatters before harvesting time. The domesticated type of T.boeoticum known as a diploid wheat of Triticum monococcum L. In this research we want to generate a high quality assembly of wild and domesticated type of Einkorn wheat. Our method is using Illumina short sequence reads assembled by both CLC, and W2RAP(Wheat/Whole- genome Robust Assembly Pipeline) software’s. In our approach …
Crop Rotations, Tillage And Cover Crops Influences On Soil Health, Greenhouse Gas Emissions And Farm Profitability, Jasdeep Singh
Crop Rotations, Tillage And Cover Crops Influences On Soil Health, Greenhouse Gas Emissions And Farm Profitability, Jasdeep Singh
Electronic Theses and Dissertations
This study assessed the effects of three levels of crop rotation: [2-yr; corn (Zea mays L.)- soybean (Glycine max L.), 3-yr; corn-soybean-oat (Avena sativa L.) or 4-yr; cornsoybean- oat-winter wheat (Triticum aestivum L.)], two tillage [conventional-till (CT) and no-till (NT)], and two winter cover cropping systems [cover crop (CC) or fallow control (NC)] on soil biochemical and physical properties, greenhouse gas emissions (GHG), microbial community composition, crop yield and farm profitability under silty clay loam soil of south eastern South Dakota. Experimental design was a randomized complete block design in a split-split plot treatment arrangement with four replications. Rotations, tillage …
Management Implications Of A Rye Cover Crop On Nutrient Cycling And Soybean Production In Southeast South Dakota: Focus On Rye Seeding Rates And Termination Timing, Benjamin Brockmueller
Management Implications Of A Rye Cover Crop On Nutrient Cycling And Soybean Production In Southeast South Dakota: Focus On Rye Seeding Rates And Termination Timing, Benjamin Brockmueller
Electronic Theses and Dissertations
Winter rye (Secale cereale L.) has become an important cover crop in South Dakota; yet, concerns over negative impacts on cash crop yields is one important limitation to the widespread adoption of winter rye in cropping systems. Two field studies were implemented at the Southeast Research Farm near Beresford, SD investigating the impacts of five seeding rates (0-90 kg ha-1) and termination timings (April 19th- May31st) with the objectives of examining the roles of winter rye management practices on soybean production and yield from nutrient cycling of nitrogen (N) and sulfur (S) in the agroecosystem. Plant, residue, …
Profiling Transcription And Retrotransposition Of Mouse L1 Subfamilies, Lingqi Kong
Profiling Transcription And Retrotransposition Of Mouse L1 Subfamilies, Lingqi Kong
Electronic Theses and Dissertations
Transposable elements, also called jumping genes, comprise almost 45% of the human genome. In contrast, only 1% of the human genome is protein-coding sequences. The function and advantages of maintaining such massive copies of transposable elements in the human genome are still unclear. Long interspersed element 1 (L1), the most substantial group and the only active autonomous transposable element in the human genome, has revealed its unique roles in many diseases. The insertional mutagenesis induced by L1 retrotransposition events could threaten human genomic stability and generate unexpected mutations. L1 overexpression has been documented in both somatic and germline cells and, …