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Department of Agronomy and Horticulture: Faculty Publications

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Articles 1471 - 1500 of 1713

Full-Text Articles in Life Sciences

Quality Traits In Forage Sorghum Harvested At Early Head Emergence And At Physiological Maturity, Jeffrey F. Pedersen, Francis A. Haskins, H. J. Gorz Jun 1983

Quality Traits In Forage Sorghum Harvested At Early Head Emergence And At Physiological Maturity, Jeffrey F. Pedersen, Francis A. Haskins, H. J. Gorz

Department of Agronomy and Horticulture: Faculty Publications

Information about the extent of variation in quality traits among plants sampled at the same developmental stage but on different dates would be useful to forage researchers. The primary purpose of this study was to obtain such information for five forage sorghum [Sorghum bicolor (L.) Moench] cultivars. Percent dry matter, crude protein, and in vitro dry matter disappearance of leaf, stem, and whole plant samples were determined for field-grown samples harvested on three different days during early head emergence (EHE) and once during physiological maturity (PM) for each cultivar. Orthogonal comparisons between EHE and PM sampling stages and among …

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False Positive Results In The Vanillin-Hel Assay Of Tannins In Sorghum Forage, M. F. Walton, Francis A. Haskins, H. J. Gorz Apr 1983

False Positive Results In The Vanillin-Hel Assay Of Tannins In Sorghum Forage, M. F. Walton, Francis A. Haskins, H. J. Gorz

Department of Agronomy and Horticulture: Faculty Publications

Vanillin-HCl procedures are widely used for the assay of tannins in plants. In attempts to adapt such procedures for use with sorghum [Sorghum bicolor (L.) Moench.] forage it was found that false positive reactions resulted, that is, red color developed in the presence of HCl with or without vanillin. Leucoanthocyanidins (monomeric proanthocyanidins) may be the constituents responsible for this red color. A "chloroform-HCl" procedure was developed for measuring leucoanthocyanidins in sorghum forage. The procedure avoids interference by chlorophyll or other chloroform-soluble constituents. With vanillin added to the solution, this procedure should also be useful for the assay of condensed …

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Variability For Traits Used To Estimate Silage Quality In Forage Sorghum Hybrids, Jeffrey F. Pedersen, Francis A. Haskins, H. J. Gorz, R. Britton Apr 1983

Variability For Traits Used To Estimate Silage Quality In Forage Sorghum Hybrids, Jeffrey F. Pedersen, Francis A. Haskins, H. J. Gorz, R. Britton

Department of Agronomy and Horticulture: Faculty Publications

The variation among 49 F1 forage sorghum [Sorghum bicolor (L.) Moench.] hybrids from a 7 X 7 cross-classified design was explored in 1979 and 1980 for the following silage traits: dry matter (DM), crude protein, in vitro dry matter disappearance (IVDMD), neutral detergent fiber, acid detergent fiber, acid detergent lignin, ammonia, lactate, and Brix of the juice from fresh stalks. Wider ranges generally were found for male than for female parental means. Means for most traits were significantly different among entries. Significant differences among hybrid means over males and over females were found for only DM, IVDMD, and …

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Yield, Vigor, And Persistence Of Sand Lovegrass [Eragrostis Trichodes (Nutt.) Wood] Following Clipping Treatments, Lowell E. Moser, L. J. Perry Mar 1983

Yield, Vigor, And Persistence Of Sand Lovegrass [Eragrostis Trichodes (Nutt.) Wood] Following Clipping Treatments, Lowell E. Moser, L. J. Perry

Department of Agronomy and Horticulture: Faculty Publications

Individual sand lovegrass [Eragrostis trichodes (Nutt.) Wood.] plants on a choppy sands range site in Nebraska’s Sandhills were clipped with 7 different harvest regimes for 3 years to determine critical defoliation times. After 3 years unclipped plants had the greatest survival rate and plants harvested only once a year on June 10 or July 10 survived better than those with other harvest regimes. Top and root yields, new tiller counts, and total non-structural carbohydrate (TNC) levels were all reduced severely with multiple harvests within one year. Sand lovegrass plants cannot tolerate close defoliation at anytime of the year although …

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Combining Ability Effects For Forage Residue Traits In Grain Sorghum Hybrids, W. M. Ross, H. J. Gorz, Francis A. Haskins, G. H. Hookstra, J. K. Rutto, R. Ritter Feb 1983

Combining Ability Effects For Forage Residue Traits In Grain Sorghum Hybrids, W. M. Ross, H. J. Gorz, Francis A. Haskins, G. H. Hookstra, J. K. Rutto, R. Ritter

Department of Agronomy and Horticulture: Faculty Publications

Combining ability effects of 10 female and 10 male inbred lines were evaluated for agronomic, grain, and forage residue traits in 100 grain sorghum [Sorghum bicolor (L.) Moench] F1 hybrids for 2 years. Traits studied were height, flowering, tillering, seed weight, yield, protein percentage, protein yield of grain, leaves, and stems; and in vitro dry matter disappearance (IVDMD) percentage and digestible dry matter (DDM) yield of leaves and stems. General combining ability (GCA) effects exceeded specific combining ability (SCA) effects for all traits except height in females and grain and leaf yields in males. Both GCA and SCA …

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Is P-Hyrdoxybenzaldehyde A Major Constituent Of Epicuticular Wax From Sorghum Bicolor Seedlings, Francis A. Haskins, H. J. Gorz Jan 1983

Is P-Hyrdoxybenzaldehyde A Major Constituent Of Epicuticular Wax From Sorghum Bicolor Seedlings, Francis A. Haskins, H. J. Gorz

Department of Agronomy and Horticulture: Faculty Publications

Free p-hydroxybenzaldehyde was not present in appreciable quantity on the surface or in the interior of week-old Sorghum bicolor shoots that had been heated to inactivate hydrolytic enzymes, nor was p-hydroxybenzaldehyde detected in epicuticular wax of greenhouse-grown sorghum ca 4.5 months old.

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Binding Of Phosphorylated Effectors By Active And Inactive Forms Of Ribulose-1, 5-Bisphosphate Carboxylase, Douglas B. Jordan, Raymond Chollet, William L. Ogren Jan 1983

Binding Of Phosphorylated Effectors By Active And Inactive Forms Of Ribulose-1, 5-Bisphosphate Carboxylase, Douglas B. Jordan, Raymond Chollet, William L. Ogren

Department of Agronomy and Horticulture: Faculty Publications

Activation of ribulose-1, 5-bisphosphate carboxylase by CO2 and Mg2+ is slow and reversible. At subsaturating concentrations of CO2 and Mg2+, positive effectors increase and negative effectors decrease the amount of active enzyme at equilibrium. Pre-equilibrium experiments indicated that both positive and negative effectors inhibit the rates of enzyme activation and deactivation. Greater than 99% inhibition of the activation and deactivation rates was observed at high effector concentrations, indicating that the binding and release of the activators CO2 and Mg2+ occur only with effector-free enzyme. The deactivation rate Ki values for the negative …

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Sod-Seeding Perennial Grasses Into Eastern Nebraska Pastures, John F. Sampson, Lowell E. Moser Nov 1982

Sod-Seeding Perennial Grasses Into Eastern Nebraska Pastures, John F. Sampson, Lowell E. Moser

Department of Agronomy and Horticulture: Faculty Publications

Eastern Nebraska and neighboring areas have large amounts of depleted pastures which consist of annual bromegrasses (Bromus spp.), Kentucky bluegrass (Poapratensis L.), and numerous broadleaf weeds. Such pastures need renovation but complete seedbed preparation is expensive and may enhance erosion. The objective of our study was to determine if sod-seeding grasses offered an alternative to complete seedbed preparation. Three weedy pastures in southeast Nebraska on a Hastings silty clay loam (fine, montmorillonitic, mesic Udic Argiustoll), a Lamo silty clay loam [fine-silty, mixed (calcareous), mesic Cumulic Haplaquoll], and a Geary silty clay loam (fine-silty, mixed, mesic Udic Argiustoll) were …

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Variability For Quality And Agronomic Traits In Forage Sorghum Hybrids, J. F. Pedersen, Herman J. Gorz, Francis A. Haskins, W. M. Ross Jul 1982

Variability For Quality And Agronomic Traits In Forage Sorghum Hybrids, J. F. Pedersen, Herman J. Gorz, Francis A. Haskins, W. M. Ross

Department of Agronomy and Horticulture: Faculty Publications

The variation among 49 F, forage sorghum [Sorghum bicolor (L.) Moench] hybrids from a 7 x 7 cross-classified design was explored in 1979 and 1980 for the following traits: dry matter (DM), crude protein (CP), in vitro dry matter disappearance (IVDMD), neutral detergent fiber (NDF), acid detergent fiber (ADF), and acid detergent lignin (ADL). Differences were found among hybrids for all traits. Parental means for males generally had a wider range of variation than for females. The means of hybrids involving the male parent, 'White Collier', were highest in IVDMD, and lowest in NDF, ADF, and ADL. IVDMD was …

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Respiration, Carbohydrate Content, And Leaf Growth Of Tall Fescue, Lowell E. Moser, J. J. Volenec, C. J. Nelson Jul 1982

Respiration, Carbohydrate Content, And Leaf Growth Of Tall Fescue, Lowell E. Moser, J. J. Volenec, C. J. Nelson

Department of Agronomy and Horticulture: Faculty Publications

Tall fescue (Festuca arundinacea Schreb.) was grown in controlled environment chambers with a 14-hour photoperiod, and then kept in darkness for 8 days in one experiment and 20 days in another. The objectives were to determine concentrations of total nonstructural carbohydrates (TNC) and relate them to leaf elongation rates (LER) and to rates of dark respiration (R,). After various times in darkness, leaf terminal meristems in vegetative tillers, root tips, and center-sections of collared leaf blades were excised, and oxygen consumption was measured.

Oxygen consumption was generally highest in terminal meristerns, intermediate in root tips, and lowest in mature …

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Divergent Selection For Hydrocyanic Acid Potential In Sudangrass, Herman J. Gorz, Francis A. Haskins, K. P. Vogel Mar 1982

Divergent Selection For Hydrocyanic Acid Potential In Sudangrass, Herman J. Gorz, Francis A. Haskins, K. P. Vogel

Department of Agronomy and Horticulture: Faculty Publications

Alteration in hydrocyanic acid potential (HCN-p) of the forage is one of the important objectives in sudangrass [Sorghum sudanense (Piper) Stapf] breeding programs. The effectiveness of recurrent phenotypic selection for increasing or decreasing HCN-p in sudangrass was evaluated in two cycles of individual plant selection in the cultivar 'Greenleaf'. In cycle 1, HCNP means of the high and low populations were higher and lower, respectively, than for Greenleaf, but only the low-HCN-p population was significantly different from Greenleaf. In cycle 2, mean HCN-p values of both populations differed significantly from Greenleaf. The average realized heritability for the two cycles …

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Performance Of Blends Of Short, Medium, And Tall Sorghum For Forage, Herman J. Gorz, Francis A. Haskins Mar 1982

Performance Of Blends Of Short, Medium, And Tall Sorghum For Forage, Herman J. Gorz, Francis A. Haskins

Department of Agronomy and Horticulture: Faculty Publications

This study was conducted to evaluate a wider range of plant types and agronomic and quality traits than had been previously reported for blends of sorghum [Sorghum bicolor (L.) Moench]. Three groups of sorghum cultivars and hybrids, each including a i-dwarf (tall), a 2-dwarf (medium), and a 3-dwarf (short) sorghum, were used in a 2-year study. Within each group the following seven blends were compared: tall (T), medium (M), and short (S); T and M; T and S; M and S; T alone; M alone; and S alone. Principal emphasis was given to total dry matter (DM) production (sum …

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Influence Of Radiation Level On Apparent Hydrocyanic Acid Potential Of Sorghum Seedlings, Francis A. Haskins, Herman J. Gorz, R. B. Clark Jan 1982

Influence Of Radiation Level On Apparent Hydrocyanic Acid Potential Of Sorghum Seedlings, Francis A. Haskins, Herman J. Gorz, R. B. Clark

Department of Agronomy and Horticulture: Faculty Publications

The hydrocyanic acid potential (HCN-p) of forage sorghum [Sorghum bicolor (L.) Moench] and sudangrass [S. sudanense (Piper) Stapf] seedlings may be estimated by a spectrophotometric procedure that is simple and rapid. As assayed by this procedure, seedlings of a number of lines appeared to have increased HCN-p when grown under increased levels of radiation. In investigations of this apparent increase, 7-day seedlings of a number of forage sorghum and sudangrass lines were grown under photosynthetically active radiation levels of about 180μE • m-2 • sec-1 (PAR-1) or 400μE • m-2 • …

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Heritability Estimates For Forage Yield, In Vitro Dry Matter Digestibility, Crude Protein, And Heading Date In Indiangrass, K. P. Vogel, Herman J. Gorz, Francis A. Haskins Sep 1981

Heritability Estimates For Forage Yield, In Vitro Dry Matter Digestibility, Crude Protein, And Heading Date In Indiangrass, K. P. Vogel, Herman J. Gorz, Francis A. Haskins

Department of Agronomy and Horticulture: Faculty Publications

Space-planted populations derived from 'Holt' and 'Oto' indiangrass, Sorghastrum nutans (L.) Nash, were used to obtain heritability estimates for forage yield, in vitro dry matter digestibility (IVDMD), crude protein, and heading date and to determine their genotypic correlations. Heritability estimates were determined by variance component analyses and by parent-progeny regression. Average narrow sense heritability estimates for the two populations were 0.43, 0.42, and 0.50, for forage yield, IVDMD, and protein, respectively. Narrow sense heritability estimates for heading date were 0.90 or larger. Genotypic correlations of yield and IVDMD were negative for the Holt population but were positive, although low, for …

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Heritability Estimates For Height, Color, Erectness, Leafiness, And Vigor In Indiangrass, K. P. Vogel, Herman J. Gorz, Francis A. Haskins Jan 1981

Heritability Estimates For Height, Color, Erectness, Leafiness, And Vigor In Indiangrass, K. P. Vogel, Herman J. Gorz, Francis A. Haskins

Department of Agronomy and Horticulture: Faculty Publications

The purpose of this study was to determine heritability estimates for plant height, leaf color, erectness, leafiness, and vigor and the correlation of these traits with one another and with forage yield, forage in vitro dry matter digestibility (IVDMD), and forage crude protein content for two indiangrass [Sorghastrum nutans (L.) Nash] populations taken from the cultivars 'Holt' and 'Oto' both of which have broad genetic bases. Narrow sense heritability estimates were obtained by using parent-progeny regression and by using variance components from the analyses of variance of half-sib families. Broad sense heritability estimates were obtained using variance components from …

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Divergent Selection For In Vitro Dry Matter Digestibility In Switchgrass, K. P. Vogel, Francis A. Haskins, Herman J. Gorz Jan 1981

Divergent Selection For In Vitro Dry Matter Digestibility In Switchgrass, K. P. Vogel, Francis A. Haskins, Herman J. Gorz

Department of Agronomy and Horticulture: Faculty Publications

The purposes of this study were to determine if phenotypic selection for in vitro dry matter digestibility (IVDMD) could be used to improve the IVDMD for switchgrass (Panicum virgatum L.) and to obtain heritability estimates of IVDMD in switchgrass. In 1973 a selection nursery of over 2,200 plants was established with transplanted seedlings of two similar Nebraska experimental strains. In 1974 about 800 plants that appeared to be agronomically superior were sampled for IVDMD. Single-plant selections for high and low IVDMD were made in each of the nursery rows. In 1976, ramets from 25 of the high-IVDMD selections and …

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Parent-Progeny Regression In Indiangrass: Inflation Of Heritability Estimates By Environmental Covariances, Kenneth P. Vogel, Francis A. Haskins, H. J. Gorz Oct 1980

Parent-Progeny Regression In Indiangrass: Inflation Of Heritability Estimates By Environmental Covariances, Kenneth P. Vogel, Francis A. Haskins, H. J. Gorz

Department of Agronomy and Horticulture: Faculty Publications

Families of two indiangrass [Sorghastrum nutans (L.) Nash] populations were used in this study with a family plot consisting of a parental clone and four half-sib progeny_ The experimental design was a randomized complete block with two replications. The regression of offspring on the parent in the same plot was used to obtain heritability estimates in which environmental covariances might be expected to inflate the parent-offspring covariance. The regression of offspring in one replication with its parent in another replication, and vice versa, was used to obtain heritability estimates whereby environmental covariances should be minimized. Heritability estimates for heading …

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Inheritance Of Flower Color In Musk Thistle (Carduus Thoermeri), M. K. Mccarty, Francis A. Haskins, H. J. Gorz May 1980

Inheritance Of Flower Color In Musk Thistle (Carduus Thoermeri), M. K. Mccarty, Francis A. Haskins, H. J. Gorz

Department of Agronomy and Horticulture: Faculty Publications

Four flower-color phenotypes were observed in a population of musk thistle (Carduus tboermeri Weinm.). This plant has been commonly referred to as C. nutans L. The four phenotypes were: purple corolla and purple pollen, pink corolla and white pollen, white corolla and purple pollen, and white corolla and white pollen. In four generations, 177 self-pollinated individuals of these four phenotypes produced 2123 progeny plants that were classified. Results support the hypothesis that three independent gene pairs were involved in deter- mining the four flower phenotypes. The gene pairs have been designated P/p, W/w, and Pi/pi. It was postulated that …

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Symbiotic Nitrogen Fixation By Legumes Requires Extra Phosphorus, Kenneth G. Cassman, A. Sheldon Whitney, Robert L. Fox Jan 1980

Symbiotic Nitrogen Fixation By Legumes Requires Extra Phosphorus, Kenneth G. Cassman, A. Sheldon Whitney, Robert L. Fox

Department of Agronomy and Horticulture: Faculty Publications

When grain legumes are grown on nitrogen-deficient soils, they may symbiotically fix enough N for good yields, even if no N fertilizer is applied. However, legumes preferentially assimilate N from the soil if this source is available to them. As a result, excess N via the soil may inhibit symbiotic N2 fixation by legumes. The physiological and morphological modifications that make symbiotic N2 fixation by legumes possible include: (I) the invasion of host roots by effective strains of Rhizobium, (2) the development of nodules to "house" the Rhizobium and, (3) the translocation of mineral nutrients and photosynthate …

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Registration Of Ten Sorghum Parental Lines, W. M. Ross, H. J. Gorz, Francis A. Haskins, O. J. Webster Jan 1980

Registration Of Ten Sorghum Parental Lines, W. M. Ross, H. J. Gorz, Francis A. Haskins, O. J. Webster

Department of Agronomy and Horticulture: Faculty Publications

The Nebraska Agric. Exp. Stn. and AR-SEA-USDA released in January 1980 10 pairs of A and B (male-sterile and maintainer) Sorghum bicolor (L.) Moench inbred lines for producing grain and forage sorghum hybrids. All A-lines have milo cytoplasm.

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Cyanogenesis In Indiangrass Seedlings, Francis A. Haskins, H. J. Gorz, Kenneth P. Vogel Dec 1979

Cyanogenesis In Indiangrass Seedlings, Francis A. Haskins, H. J. Gorz, Kenneth P. Vogel

Department of Agronomy and Horticulture: Faculty Publications

In a study of seedlings of 72 entries representing 39 species, 14 genera, and 2 tribes of grasses, only entries of the genus Sorghastrum were found to contain the cyanogenic glucoside, dhurrin [(S)-p-hydroxymandelonitrile β-D-glucopyranoside]. Dhurrin was found in seedlings of each of the 10 entries of indiangrass [Sorghastrum mutans (L) Nash] and the three entries of S. pellitum.
Seedlings of five indiangrass cultivars were assayed for dhurrin concentration. Levels expressed as HCN potential (HCN-p) ranged from about 900 ppm for 'Holt' to about 1,200 ppm for 'Llano' and 'Oto'.
Further studies with Oto seedlings indicated that …

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Registration Of N-2 Red Clover Germplasm, H. J. Gorz, G. R. Manglitz, Francis A. Haskins Jun 1979

Registration Of N-2 Red Clover Germplasm, H. J. Gorz, G. R. Manglitz, Francis A. Haskins

Department of Agronomy and Horticulture: Faculty Publications

N-2 red clover (Trifolium pratense L.), carrying high levels of resistance to the yellow clover aphid (Therioaphis trifolii (Monell)) and the pea aphid (Acyrthosiphon pisum (Harris)), was released to researchers and commercial breeders in July 1978. It was developed by five cycles of phenotypic recurrent selection for yellow clover aphid (yeA) resistance and three such cycles for pea aphid (PA) resistance. Thirty-five diverse sources of germplasm, used in the initial evaluations for aphid resistance, were listed previously.

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Selection For Yellow Clover Aphid And Pea Aphid Resistance In Red Clover, H. J. Gorz, G. R. Manglitz, Francis A. Haskins Apr 1979

Selection For Yellow Clover Aphid And Pea Aphid Resistance In Red Clover, H. J. Gorz, G. R. Manglitz, Francis A. Haskins

Department of Agronomy and Horticulture: Faculty Publications

Most red clover (Trifolium pratense L.) cultivars are susceptible to attack by the yellow clover aphid (Therioaphis trifolii Monell) and the pea aphid (Acyrthosiphon pisum Harris). Starting with 27 yellow clover aphid-resistant plants selected from a wide diversity of germplasm, phenotypic recurrent selection was used to improve resistance to the two aphids. Through five cycles of testing and selection for yellow clover aphid resistance and three such cycles for pea aphid resistance, a synthetic, 'N-2', was developed that had a high level of resistance to both aphids.

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Effects Of Glyphosate On Highbush Blueberry (Vaccinium Corymbosum L.), Laurie Hodges, Ronald E. Talbert, J. N. Moore Feb 1979

Effects Of Glyphosate On Highbush Blueberry (Vaccinium Corymbosum L.), Laurie Hodges, Ronald E. Talbert, J. N. Moore

Department of Agronomy and Horticulture: Faculty Publications

Five-year-old 'Collins' highbush blueberries were treated in August with glyphosate (N-phosphonomethyl glycine) at 0.36, 3.6 and 7.2 g/liter acid equivalent as a spot treatment alone or with pruning or applying paraquat (1, 1'-dimethyl-4, 4'-bipyridinium ion) at 1.2 g/liter to remove green tissue prior to glyphosate application. Initial response was terminal dieback of young canes. Symptoms the following spring included additional terminal dieback, leaf and cane morphological aberrations, and elongation of the flower corolla. One year after treatment, regrowth was normal. There was no effect on blueberry yield the season following treatment.

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Dhurrin In Sorghastrum Nutans, H. J. Gorz, Francis A. Haskins, Richard Dam, Kenneth P. Vogel Jan 1979

Dhurrin In Sorghastrum Nutans, H. J. Gorz, Francis A. Haskins, Richard Dam, Kenneth P. Vogel

Department of Agronomy and Horticulture: Faculty Publications

The isolation of dhurrin, the cyanogenic glycoside of Sorghum, was reported by Dunstan and Henry in 1902 [1]. This compound, now known to be (S)-p-hydroxymandelonitrile β-D-glucopyranoside [2, 3], was the first cyanogenic glycoside isolated from a representative of the Gramineae [4]. The occurrence of other cyanogenic glycosides in certain grasses is now recognized [4, 5], but according to Fat [4], Sorghum remains the only genus in which the presence of dhurrin has been firmly established. In this report, evidence is presented indicating that dhurrin also occurs in seedlings of the warm season perennial prairie grass, indiangrass, Sorghastrum …

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Combining Ability In Forage Sorghum Hybrids, W. M. Ross, Herman J. Gorz, Francis A. Haskins, K. D. Kofoid Jan 1979

Combining Ability In Forage Sorghum Hybrids, W. M. Ross, Herman J. Gorz, Francis A. Haskins, K. D. Kofoid

Department of Agronomy and Horticulture: Faculty Publications

Two cross-classified hybrid forage sorghum [Sorghum bicolor (L.) Moench] experiments were conducted at Mead, NE: Experiment 1 (3 females x 8 males), in 1973-75, and Experiment 2 (13 females x 2 males), in 1974-75. Data were collected on plant height, days to bloom, forage yield, percent dry matter (DM), percent protein, and in vitro dry matter disappearance (IVDMD) in both experiments and percent Brix in Experiment 1. Differences among hybrids averaged over females or over males were significant for each trait in one parental group or the other in each test except IVDMD in Test 1 and DM in …

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Carbon-L 4 Translocation In Three Warm- Season Grasses As Affected By Stage Of Development, Walter H. Fick, Lowell E. Moser Jul 1978

Carbon-L 4 Translocation In Three Warm- Season Grasses As Affected By Stage Of Development, Walter H. Fick, Lowell E. Moser

Department of Agronomy and Horticulture: Faculty Publications

Radioactive carbon dioxide was utilized to trace carbohydrate translocation in blue grama, sideoats grama, and switchgrass. Tagged tillers retained 77% of their fixed carbon following a 24-hour translocation period. The roots were the strongest sink of assimilate, receiving 76.2% of the total 14C translocated. The pre-elongated and elongated tiller fractions received 16.3% and 7.5% of the 14C translocated, respectively. Major differences among the grasses became evident upon examination of relative total activity (RTA) ratios in elongated/pre-elongated tillers and root/shoot fractions. Switchgrass produced many more elongated tillers and had the highest elongated/pre-elongated RTA ratio. Blue grama had the greatest weight …

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Assay Of P-Hydroxybenzaldehyde As A Measure Of Hydrocyanic Acid Potential, Herman J. Gorz, W. L. Haag, J. E. Specht, Francis A. Haskins Jul 1977

Assay Of P-Hydroxybenzaldehyde As A Measure Of Hydrocyanic Acid Potential, Herman J. Gorz, W. L. Haag, J. E. Specht, Francis A. Haskins

Department of Agronomy and Horticulture: Faculty Publications

A method of assessing the hydrocyanic acid potential (HCN-p) of sudangrass [Sorghum sudanense (Piper) Stapfl and sorghum [S. bicolor (L.) Moench] seedlings is described. This procedure is based on the determination of p-hydroxybenzaldehyde (p-HB), which is released upon hydrolysis of dhurrin, the cyanogen normally present in plants of Sorghum species. Extraction and hydrolysis of dhurrin are accomplished by autoclaving young leaf tissue in water. The content of p-HB in the aqueous extract is then determined by spectrophotometric assay in alkaline solution at 330 nm. Uniform samples for the comparison of widely divergent genotypes are obtained by using …

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Callus And Suspension Cultures Of Melilotus Alba Tissues And Cells, Tomoaki Taira, Francis A. Haskins, Herman J. Gorz May 1977

Callus And Suspension Cultures Of Melilotus Alba Tissues And Cells, Tomoaki Taira, Francis A. Haskins, Herman J. Gorz

Department of Agronomy and Horticulture: Faculty Publications

These studies were designed to define conditions for the satisfactory establishment and maintenance of sweetclover (Melilotus alba Desr.) callus and cell suspension cultures. Callus was derived from cotyledons and hypocotyls of germinated sweetclover seeds of various genotypes. Under the conditions used, 2,4-dichlorophenoxyacetic acid (2,4-D) was effective (optimal concentration ca I mg/liter) for callus induction; other auxins and cytokinins were ineffective. Best callus growth occurred at a 2,4·D concentration of about 2 mg/liter and a sucrose concentration of 2%. Callus growth was improved by casein hydrolysate (0.9 g/liter). Glutamic acid appeared to be the most important single amino acid in …

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Summary Of Dry Bean Conference Jan 1977

Summary Of Dry Bean Conference

Department of Agronomy and Horticulture: Faculty Publications

Four years earlier a similar dry bean conference was held in Gering. The conference was not structured but was an open discussion. The greatest value of that conference was the stimulus it provided the staff doing research and extension with dry beans. Much scientific work has been done since that conference and it was again apropos to review the status of the research and extension activities. We therefore gathered together in the North Platte Airport Conference Room and spent the day discussing many aspects of the dry bean industry and how each of us relate to it. We needed to …

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