Life Sciences Commons^™

Evaluation Of Herbicides For Suppression Of Rhizome Growth From Johnsongrass (Sorghum Halepense L.) In Corn And Soybeans, John Oakes

Masters Theses & Specialist Projects

Johnsongrass (Sorghum halepense L.) presents a serious problem in the production of corn and soybeans in Kentucky.

Various rates of the thiocarbamates, dinitroanilines, carbamates, amides, ureas, and triazines were used in association with the crops corn (Zea mays L.) and soybeans (Glycine max L.) to determine the degree of suppression on rhizomatous johnsongrass infesting the previously named crops.

Suppression was determined by selecting areas within each treated plot, counting the number of culms, then harvesting johnsongrass rhizomes. Dry weights of rhizomes were then determined as a measure of rhizome suppression.

Results show that of …

Movement Of Soil Nitrate Through A Pembroke Soil As Affected By Tillage Method And Time Of Nitrogen Application, Noel T. Johnston

Masters Theses & Specialist Projects

Of all the plant nutrients, nitrogen has been subjected to the most extensive study. The amount of inorganic nitrogen in the soil is small while the quantity needed annually by crops is comparatively large. Of the macronutrients usually applied in commercial fertilizers, nitrogen seems to have the quickest and most pronounced effect on plant growth.

In applying the nitrogen fertilizer for crop use, one must be concerned with placement, form, and availability, and with keeping the fertilizer where it is placed throughout the critical part of the growing season. The nitrogen supply molded by non-leguminous plants is of extreme importance …

Evaluation Of Glyphosate In No-Till Double Crop Soybeans Using Varying Volumes & Pressures, Charles Rogers

Masters Theses & Specialist Projects

No-tillage production of crops has developed and become widespread since the late 1950/s. No-till is dependent upon the use of broad spectrum herbicides to kill weeds already growing at planting time. Until recently, this has almost exclusively been paraquat (1,1'-dimethy1-4,4'-bipyridinium ion). Glyphosate [N-(phosphonomethyl)glycine] is a new herbicide which has great potential for use in no -tillage production.

Soybeans (Glycine max L.) were planted in the stubble after wheat (Triticum aestivum L.) was harvested. All plots received applications of the residual herbicides alachlor [2-chioro-2',6'-diethyl-N-(methoxymethyl)acetanilide] and linuron [3-(3,4-dichlorophenv1)-1-methoxy-1-methylurea]. Each plot also received either paraquat or glyphosate or was designated as …

Effects Of Time Of Application Of Glyphosate In The Control Of Johnsongrass, Marlin Moody

Masters Theses & Specialist Projects

Investigations were conducted to determine the effects of the herbicide glyphosate and its time of application on the control of johnsongrass.

Johnsongrass plots were divided into areas designated undisturbed, spring plowed, and clipped. These areas were compared to determine differences in control of johnsongrass when using glyphosate with different management procedures. Glyphosate was applied at weekly intervals and treatment effects were statistically analyzed. There were three replications of each treatment.

It appears from the results that:

1. Glyphosate kills approximately 100% of the johnsongrass topgrowth regardless of the application date.

2. Height of the johnsongrass plant at the time of glyphosate application …

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Kentucky Warbler (Vol. 52, No. 4), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

Ua77/1 Western Alumnus, Vol. 47, No. 1, Wku Alumni Relations

WKU Administration Documents

Alumni magazine published by WKU. This issue has the following articles:

• Orientation - Advisement - Registration Programs
• Armstrong, Don & Jim Snodgrass. Prize Dairy Herd
• Conway, Sheila. Nursing Goes Baccalaureate
• Snodgrass, Jim. The Hub of the Hill - Goal Post Restaurant
• Oakes, John. Respect Grows for Western Fine Arts Festival
• Krull, Carl. Special Olympics
• Cunningham, Doyle. The Public Service Institute
• Jason Brady's World
• Conway, Sheila. 2076 America: The Next 100 Years
• Allan Ostar Praises Western Spirit
• Students Pick Yell Leaders
• Harvey, Debbie. Christy Vogt Takes Over Associated Student Government
• Award for Public Affairs
• Joe Uveges Publishes Textbook
• James Pearse President-Elect …

Kentucky Warbler (Vol. 52, No. 3), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

Effect Of Matallic Ions, B-Vitamins, & Amino Acids On The Production Of Trans-2, 3-Epoxysuccinic Acid By Paecilomyces Varioti, Eleanor Ling

Masters Theses & Specialist Projects

The nutritional conditions which permit maximum yield of trans-2,3- epoxysuccinic acid by Paecilomyces varioti NPRL 1123 were investigated. Copper and iron ions were added to decationized fermentation media to determine the optimum concentration and ratio of the ions for the accumulation of trans-2,3-epoxysuccinic arid. P-vitamin and amino acid studies were done by single omission of the growth factors in culture media. Organic acids in the cultures were recovered by ether extraction and identified by gas chromatography.

In addition to t-ans-2,3-epoxysuccinic acid, succinic acid, malic acid, and tartaric acid were also found in culture filtrates. The results indicated that with a …

The Metabolism Of Glucose By The Combined Use Of Gluconobacter Suboxydans & Pseudomonas Fluorescens, Kathy Ware

Masters Theses & Specialist Projects

Metabolism of glucose by G. suboxydans and P. fluorescens in pure and mixed culture was characterized in 10% glucose-yeast extract medium in presence of calcium carbonate. A pattern between viable cell counts and copper-reducing compounds was established. A decrease in viable cell counts of the two bacteria was observed after the maximum accumulation of 5- and 2- oxogluconate. P. fluorescens was found to have a faster growth and rate of production of 2-oxogluconate than the growth and production rate of 5-oxogluconate by G. suboxydans.

It was found that P. fluorescens grew on nutrient agar at pH 7.5, but G. suboxydans …

The Wavelength Dependency Of Ultraviolet Induction Of Simian Virus 40 From A Transformed Line Of Baby Hamster Kidney Cells, Timothy Bichenbrenner

Masters Theses & Specialist Projects

For our investigation, an established line (E line) of baby hamster kidney cells which was transformed by the incorporation of Simian Virus 40 into the cellular genom was used. The induction of this virion by irradiating these cells with ultraviolet light was studied. The purpose of this thesis was to determine the wavelength dependency (at four selected ultraviolet mercury emission lines) of the induction of the SV40 genome from these cells. The viral titres of induction were measured by the agar overlay plaque assay method and by the fluorescent antibody microassay technique.

At the four wavelengths tested 265 nm and …

Autecological & Populational Investigation Of Carpinus Caroliniana Walt., Gordon Wardell

Masters Theses & Specialist Projects

Analysis of habitats containing Carpinus caroliniana in two distinct physiographic regions of Kentucky revealed that this species has a wide range of tolerance to light intensities and soil pH. Species most commonly associated with Carpinus within the two physiographic regions were Platanus occidentalis, Acer saccharum, Liquidambar styraciflua, Fraxinus spp. and Fagus grandifolia.

Morphological and anatomical investigations of populations representing the geographic distribution of Carpinus caroliniana in the United States indicated that the more northern population had heavier fruit, larger bracts, and secondary wood with higher specific gravity.

Growth chamber experiments involving dormant buds collected from natural habitats indicated differences …

Constitutive Nature Of 5-Ketogluconate Reductase Of Gluconobacter Suboxydans, Vahag Marootkhanian

Masters Theses & Specialist Projects

NADP⁺-dependent 5-ketogluconate reductase (D-Gluconate:NADP⁺ oxidoreductase; E.C.I.I.I.69.) of Gluconoacter suboxydans was examined and determined to be constitutive in nature. Crude, cell -free enzyme extracts were prepared by sonication of cells grown on 2.0'% glucose and 2.0% ethanol in various experiments. The specific activity of 5-ketogluconate reductase in the crude enzyme extracts of cells grown under various conditions was determined. The specific activity remained relatively constant strongly indicating the constitutive nature of the enzyme.

Electrophoretic Heterogeneity Of Nicotinamide Adenine Dinucleotide Phosphate-Dependent 5-Ketogluconate Reductase Of Gluconobacter Suboxydans, Sharon Dianis

Masters Theses & Specialist Projects

NADP⁺-dependent 5-ketogluconate reductase (D-Gluconate:NAD(P)⁺ oxidoreductase E.C. 1.1.1.69) isozymes were detected in cell-free extracts of Gluconobacter suboxydans (American Type Cultures Collection, Bethesda, Maryland, strain 621). Cell-free enzyme extracts were prepared from cells grown in media which contained glucose, gluconate, glycerol, sorbitol, or mannitol as the sole carbon source in 2% concentration. The extracts were fractionated using analytical polyacrylamide disc-gel electrophoresis. Gels were stained specifically for 5-ketogluconate reductase. Three isozymes were present in each extract from cells grown on the five different carbon sources. Gels stained in a reaction mixture which contained glycerol exhibited two of the isozymes.

An …

Radioprotection Of Oral Cavity Structures By S-2-(3-Aminopropylamino) Ethyl Phosphorothioate (Wr-2721), Ronald King

Masters Theses & Specialist Projects

Studies reporting a high concentration of WR-2721 in mouse salivary glands led to our studies of possible radioprotection of these glands by this drug from ionizing radiation. Oral effects of radiation in the presence of WR-2721 were studied in mice and dogs. Histological evaluation of mouse salivary glands irradiated with 1000 rads of ⁶⁰Co showed essentially no difference between control and experimental animals. Almost full regeneration of the serous salivary components occurred by 6 months in both groups and neither group had changes in the mucous glands. The use of higher doses of radiation in the mouse was prevented …

Population Dynamics And Tackle Catch Susceptibility Of The Smallmouth Buffalo, Ictiobus Bubalus (Rafinesque), In Rough River Lake, Kentucky, Alan Waite

Masters Theses & Specialist Projects

The population of the smallmouth buffalo, Ictiobus bubalus (Rafinesque), was studied in Rough River Lake, Kentucky, from July, 1974, through June, 1975. A total of 758 individuals were collected with 587 being marked and returned.

From calculations made using poison population sample data, population size was estimated to be between 150,00 to 225,000 adult fish or 29 to 44 fish per acre. Scales were studied to determine age and through back calculation, the total length of each fish at the time of each annulus formation determined. Ten year ciasses were observed with the majority of the fish in age groups …

Kentucky Warbler (Vol. 52, No. 2), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

Forest Composition Of Rock Creek Gorge A Virgin Forest In Laurel County, Kentucky, Margaret Ringland

Masters Theses & Specialist Projects

Rock Creek Gorge in Rock Creek Natural Landmark in Laurel County, Kentucky is a deep narrow gorge dominated by Tsuga canadensis (L.) Carr., Oxydendrum arboreum (L.) DC., Betula lenta L. and Liriodendron tulipifera L. Rhododendron maximum L., dominating the understory, is also an important constituent of the gorge. An analysis of the forest composition showed the community to be stable and climax. The dominant species were found in sapling and seedling size classes. The vegetation varied slightly between the east facing and west facing slopes. Hemlock did not attain as high an importance value on the west facing slope, although …

Iron Requirement Of Pigs Farrowed In Metal Farrowing Stalls, Rebecca Walker

Masters Theses & Specialist Projects

Pigs farrowed and reared in metal farrowing stalls were studied to determine the need for iron. Three treatments were studied, namely—T-O (pigs given no iron injection), T-1 (pigs given 1 iron-dextran injection at 2 days of age) and T-2 (pigs given 2 injections of iron-dextran, the first at day 2 and the second at day 7). Hemoglobin levels were analyzed to determine the incorporation of iron in the bloodstream in the form of hemoglobin. Pig weights were used as a measure of production.

The hemoglobin levels did not differ (p>0.1) among the 3 treatments at birth, 4 days, 6 …

Evaluation F The Germ Tube Test & Other Tests For The Differentiation Of Candida Albicans From Candida Stellatoidea, Daniel Rogers

Masters Theses & Specialist Projects

Candida albicans is a pathogenic yeast often encountered in the medical laboratory. A survey taken by mail of 110 Kentucky hospital and clinical laboratories demonstrated that the germ tube test is the most frequently used method for the identification of this organism. Earlier workers have found that this technique does not distinguish between C. albicans and C. stellatoidea. Various modifications of the germ tube test that could possibly be used in the differentiation of these two taxa were investigated. Parameters examined included the effects of oxygen tension, CO₂, increased temperature, serum dilution, glucose concentration, and a synthetic …

Ua12/2/19 Earth Week, Energy For Student Awareness

WKU Administration Documents

Newspaper created by the Energy for Student Awareness group regarding Earth Week and environmental issues.

Kentucky Warbler (Vol. 52, No. 1), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

Ultraviolet Radiation Effects On Rotifers, Syed Asim Hussain

Masters Theses & Specialist Projects

The purpose of this investigation was to study ultraviolet radiation effects on rotifers. The rotifer studied was Philodina acuticornis odiosa Milne. Isolation cultures were used to grow the rotifers individually in separate U-plate depressions. The source of ultraviolet radiation consisted of four mercury germicidal lamps used in combination to deliver a uniform flux of 315 uW/cm² over the sample. Five groups of rotifers, one control and four experimental groups of 96 each, were used. The rotifers used were 2-10 hours of age at the beginning of the experiment. The experimental groups were exposed to uv radiation for 3 minutes, …

Tobacco Farming: The Persistence Of Tradition, Eugene Umberger Jr.

Masters Theses & Specialist Projects

The culture of tobacco has been associated with the history of Kentucky almost from the beginning and remains to this day a vital force in the state’s economy. In this age of scientific and technological advances – of increasing automation – we find that in tobacco farming, hand labor still figures prominently in the production of a major staple crop. This has resulted in the retention of traditional method, technology and terminology, long since lost in the culture of other crops which lent themselves more easily to mechanization.

The study is divided into three parts. Chapter I deals briefly with …

Partial Purification & Kinetic Properties Of Nicotinamide Adenine Dinucleotide Phosphate-Dependent Alcohol Dehydrogenase Of Phycomyces Blakesleeanus, Thomas Hartz

Masters Theses & Specialist Projects

NADP⁺-dependent alcohol dehydrogenase (alcohol:NADP⁺:oxidoreductase, EC 1.1.1.2.) was partially purified from a cell-free extract of Phycomyces blakesleeanus (+ mating type, Vanderbilt strain). The fungus was grown in shake cultures containing glucose-asparagine broth, and crude cull-free enzyme extracts were prepared. The crude enzyme extracts were partially purified by a combination of ammonium sulfate precipitations and heat precipitation. Purification resulted in a 40;5 recovery of the enzyme with an increase in specific activity from 0.64 units per milligram of protein to 0.25 units per milligram. The partially-purified enzyme extracts were used to determine the kinetic properties and substrate specificity …

Kentucky Warbler (Vol. 51, No. 4), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

Kentucky Warbler (Vol. 51, No. 3), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

Comparative Bioenergetics Of Insects From Young & Old Plant Ecosystems, Joyce Wright

Masters Theses & Specialist Projects

Caloric values of insects and arachnids from two woods and two meadow ecosystems were compared. No significant difference was found between the orders Diptera, Homoptera, Orthoptera, Hemiptera, Hymenoptera, or Coleoptera. Caloric values of the class Arachnida were significantly higher in the woods ecosystem at the .05 level of significance. The order Lepidoptera differed at the .01 level of significance and was also higher in the woods samples. For all orders combined the caloric values of the woods samples were higher than the meadow samples at the .001 level of significance.

No consistent pattern was found in seasonal variation in caloric …

The Life History Of The Teardrop Darter, Etheostoma Barbouri Kuehne And Small, In Kentucky, Rory Flynn

Masters Theses & Specialist Projects

The life history of the teardrop darter, Etheoctoma (Catonotus) barbouri Kuehne and Small, was intensively studied from September, 1972, through August 1973. A total of 299 teardrop darters was seined from two isolated populations in Alexander and Little Muddy Creeks, Kentucky. These darters are known only to occur in second, third or fourth order tributaries of the Green River in Kentucky and Tennessee, and normally seek protection afforded by rocky or vegetated edges of such streams.

Gonosomatic ratios indicated that teardrop darters spawned from late March through late May in waters 12.5 - 15.5 C. Young females developed primary ova …

Lethatlity Of And Elicitation Of Protective Antibody By Vibrio Parahaemolyticus Attc 17803, Richard Carlucci

Masters Theses & Specialist Projects

The LD₅₀ of Vibrio parahaemolyticus ATCC 17803 for 13- to 18-g male mice, strain ICR, as determined by the 50% endpoint method, was found to be an estimated 7 x 10⁷ CFU, when administered intraperitoneally in 0.5 ml of 24-h broth culture, O. D. 0.5 at 650 nm, diluted to 10^-0.72.

The elicitation of protective antibody by this bacterium in male New Zealand White rabbits was demonstrated by testing control, baseline, and hyperimmune rabbit sera for their protective effect against 2 LD₅₀ of the bacterium administered intraperitoneally in 13- to 18-g male mice, strain ICR. …

Structure & Composition Of A Climax Forest System In Boone County, Kentucky, Michael Held

Masters Theses & Specialist Projects

The structure and composition of a 52 hectare mature mesic hardwood forest in Boone County, Kentucky was studied during 1973-74. Acer saccharum was the dominant tree species of the entire forest, with Fraxinus americana as the subdominant. In the understory vegetation, Acer and Fraxinus were among the dominant genera, thus this forest system can be described as being at the climax stage of development. A previously cleared area in the forest was also analyzed. It was found that the dominant tree species of the canopy were also dominant in the genera of the understory in the disturbed area, indicating a …