Life Sciences Commons^™

The Relationship Between The Murine Primary Immune Response To Sheep Red Blood Cells & Heterosis, Russell Gailor

Masters Theses & Specialist Projects

Two inbred strains of mice, BALB/c and C57BL, were crossbred to produce Fl hybrids. This breeding scheme ensured a high degree of genetic heterozygosity as exemplified by the fact that the average weight of the Fl hybrids at 10 weeks of age was 10.1 percent greater that that of their contemporaries in the inbred parental strains.

The three lines of mice were then challenged with a 0.5 ml intraperitoneal injection of two-percent sheep red blood cells (SRBC). After a 15-day period the mice were bled and their sera were microtitrated for anti-SRBC agglutinin and the titers statistically analyzed. The Fl …

Interview With Craig Allen Smith (Fa 107), Manuscripts & Folklife Archives

Folklife Archives Oral Histories

Transcription of interview with Craig Allen Smith conducted by Jeffrey Brian Hagan on 3 November 1989. From folk studies student project titled “Farming Folklore,” concerning farming traditions and practices in Kentucky.

Kentucky Warbler (Vol. 65, No. 4), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

Hagan, Jeffrey Brian (Fa 107), Manuscripts & Folklife Archives

Folklife Archives Finding Aids

Finding aid and full-text scan of paper (Click on “Additional Files” below) for Folklife Archives Project 107. Farming folklore. Interviews, November 1, 3, 1989, with William M. Cecil and Craig Allen Smith concerning farm occupational lore and duties. Includes paper discussing collector's fieldwork experiences.

Interview With William M. Cecil (Fa 107), Manuscripts & Folklife Archives

Folklife Archives Oral Histories

Transcription of interview with William M. Cecil conducted by Jeffrey Brian Hagan on 1 November 1989. From folk studies student project titled “Farming Folklore,” concerning farming traditions and practices in Kentucky.

Kentucky Warbler (Vol. 65, No. 3), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

A Comparison Of Chemical Composition & Fermentation Patterns Of Alternative Silages To Whole Plant Corn Silage, Susan Fox

Masters Theses & Specialist Projects

A comparison of the relationship of fermentation to chemical composition was made for forages which were wilted and ensiled at 35 to 45 percent dry matter. Trial I consisted of three forages ensiled in October, 1981: interseeded soybeans and grain sorghum. whole plant corn with added anhydrous ammonia, and whole plant corn with shelled corn added at a rate of 150 kg/t of fresh forage. Trial II consisted of two forages ensiled in 1982: interseeded soybeans and pearlmillet, and wheat. Temperatures of fermentation were collected, and chemical composition during the first 25 days of fermentation analyzed.

Production data were also …

Ua66/6/2 Bionews From The Hill, Wku Biology

WKU Administration Documents

Newsletter created by and about WKU Biology highlighting the activities of faculty, students and alumni.

Studies On I) Dry Matter And Nitrogen Disappearance Of Six Soybean Protein Products In Situ And Ii) Contamination Of In Situ Dry Matter And Nitrogen Disappearance With Acid Detergent Fiber, James Coomer

Masters Theses & Specialist Projects

In experiment I), dry matter disappearance (DMD) and nitrogen disappearance (ND) of raw soybeans (RAW), solvent extracted soybean meal (SBM), heat treated whole soybeans (HT), mechanically extracted soybean meal (MEX), dry extruded soybeans (DEX), and wet extruded soybeans (TEX), were studied in situ for times of 3, 6, 12 and 24 h of rumen exposure. Five gram, air dry, samples were suspended in the rumen of a lactating Holstein cow fed a total mixed ration twice daily. The percent DMD for 24 h was as follows: RAW-85.9; SBM-56.6; HT-39.0; MEX-40.2; DEX-28.0; TEX-43.3. The greatest DMD was observed with RAW and …

Isolation & Characterization Of A Type I Topoisomerase From The Hypocotyls Of Etiolated Soybeans, Rick Dye

Masters Theses & Specialist Projects

Topoisomerases are enzymes of critical biological significance. Despite this fact, little data specific to the topoisomerases of higher plants has been published. This research was undertaken to isolate and characterize a higher plant topoisomerase from soybean hypocotyls to further the understanding of the structure and function of these enzymes.

Nuclei were isolated from frozen hypocotyls of 4 day old etiolated soybeans by homogenization and centrifugation, then lysed by gentle stirring in the presence of 1.5 mM ammonium sulfate. The resultant extract was desalted and purified by column chromatography on DEAF sepharose, 5-200 sephacryl and CM cellulose. The components of the …

Kentucky Warbler (Vol. 65, No. 2), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

Effect Of Phosphorus Placement Methods On The Yield & Quality Of Tomatoes, Timothy Hambrick

Masters Theses & Specialist Projects

Research was undertaken in the summer and fall of 1988 to determine the effect of phosphorus placed below, below and to the side, banded and incorporated, and broadcast on the yield and quality of tomatoes.

The results of the field study were not statistically significant due to high levels of available soil phosphorus. However, there were trends toward higher total yields, higher yield of number one grade tomatoes, and higher tissue phosphorus levels when phosphorus was placed in a concentrated zone or band as contrasted with the more popular broadcast method.

The effect of phosphorus placement in the greenhouse was …

The Effects Of Spacings & Cultivars On Biological Yields, Yields, Component Of Yield & Harvest Index Of Green Bush Snap Bean (Phaseolus Vulgaris L.), Widodo

Masters Theses & Specialist Projects

The effects of cultivars and plant spacings on biological yield, seed yield, seeds per pod, average seed weight, and harvest index of beans (Phaseolus vulgaris L.) were studied in 1987 and 1988. Two cultivars, 'Blue Lake 27' and 'Kentucky Wonder 125' and four plant spacings -- 7, 14, 21, and 28 cm, constituted eight treatments that were arranged in a split-plot with four replications. Each experimental unit or hill consisted of five plants -- four in the corners and one in the center of a square. Spacings treatment were established by varying the distance between the center plant …

Partial Purification & Characterization Of A Carboxylesterase From The Greater Wax Moth Larva, David Garst

Masters Theses & Specialist Projects

Various properties of a carboxylesterase of the greater wax moth larva, Galleria melionella (L.), and a commercial carboxylic ester hydrolase (EC 3.1.1.1) from porcine liver were examined. The larval enzyme was partially purified. Esterase activities were determined with acetylsalicylic acid, 0-acetyl-m-hydroxybenzoic acid, and 0-acetyl-phydroxybenzoic acid in tris buffer (0.05 M tris, 0.1 M EDTA, pH 7.85). The larval enzyme was partially purified by a combination of acetone powder preparation, ammonium sulfate precipitation, anion exchange chromatography, 0-200 gel filtration, and polyacrylamide gel electrophoresis (PAGE).

The acetone powder preparation was initially obtained and tested for esterolytic activity. The ammonium sulfate fractionation greatly …

Kentucky Warbler (Vol. 65, No. 1), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

Historical Biogeography: Geography As Evolution, Evolution As Geography, Charles H. Smith

DLPS Faculty Publications

Despite a number of advances in recent years, biogeography remains a field with a poorly developed philosophical core. As a result, its historical and ecological sides remain as isolated from one another as ever. In this essay I argue that a more unified approach to biogeographic studies will become possible only when workers realise that it is necessary to reject absolute space, "geography as handmaiden" approaches to distribution problems in favour of structuralist models compatible with both probabilistic spatial interaction and deterministic phylogenetic kinds of thinking. Pros and cons of regionalist, vicariance, and panbiogeographic approaches are weighed in this regard; …

Ua35/11 Student Honors Research Bulletin, Wku Honors Program

WKU Administration Documents

The WKU Student Honors Research Bulletin is dedicated to scholarly involvement and student research. These papers are representative of work done by students from throughout the university.

• Coates, Alan. Management's Attitude Toward Collective Bargaining
• Frew, Becky. The Effects of Personality Type and Social Support on Instances of Job Stress
• Green, Laura. Earth Sheltered Housing
• Gregory, Vicky. Fraud and Abuse by Medical Providers in Medicare and Medicaid
• Hignite, Susan. Japanese Students in Kentucky Classrooms
• Hodum, Martha. The Mathematically Gifted Child
• Jones, Rodney. Superman to Censorship: America's Romance with the Comic Book
• Marrs, Shawn. Romanticism and Unromanticism in Sir Gawain and the …

Ua64/10/5 Scrapbook, 1977-1989, Wku Recreation Majors' Club

Student Organizations

Scrapbook created by the WKU Recreation Majors' Club for 1977-1989. Book includes photographs of events and outings, invitations, correspondence and clippings.

Performance & Carcass Traits Of Swine Of Four Different Phenotypes & Three Usda Grades, Jon Althaus

Masters Theses & Specialist Projects

The purpose of this study was to compare the performance and carcass characteristics of swine of four different phenotypes and three USDA grades.

Hogs were placed into phenotypic groups of 24 each and penned by group. These pens were ideal, cutability, light muscled, fat, VS US and US The hops were fed a 16% corn and soybean meal ration fortified with appropriate minerals and vitamins. They were housed in a total confinement curtain-sided Kentucky plan building with a partially slatted floor. The hogs were weighed at 6-week intervals throughout the project to monitor average daily gain and pen feed efficiency. …

Isolation & Characterization Of Dna Polymerase Alpha And Gamma From Turnips (Brassica Rapa) And Etiolated Soybeans (Glycine Max), Donna Hill

Masters Theses & Specialist Projects

DNA polymerase alpha, the enzyme involved in nuclear DMA replication, and DNA polymerase gamma, the enzyme involved in organellular DNA replication, were isolated and purified from soybean and turnip. The enzymes were characterized following ammonium sulfate precipitation, DEAF-cellulcse, phosphocelluloca, and hydroxylapatite chromatography, and by non-denaturing polyacrylamide gel electrophoresis. Protein banct were electropluted and the enzymes characterized using kinetic studies and sensitivity to divalent cations and inhibitors. Molecular weight and subunit composition studies indicated a molecular weight for the catalytic subunit of DNA polymerase alpha in soybean and turnip to be 46kDa. DNA polymeraqe gamma was composed of a catalytic subunit …

The Short Term Effects Of Manganese Toxicity On Isocitrate Dehydrogenase In Wheat & Tobacco, Juergen Pfeiffer

Masters Theses & Specialist Projects

The short term effects of high manganese levels (80 mg/L) on wheat and tobacco isocitrate dehydrogenase (IDH) activity were compared. The change in activity was then compared to the concentrations of manganese in the tissues.

As the time of manganese exposure increased, the fresh weight IDH activities in wheat and tobacco decreased. In contrast both control groups had similar enzyme activities every 6 hr over the 24 hr test period. The decrease in IDH activity of wheat and tobacco plants in experimental groups seemed to be related to an increase of the manganese in the leaf tissue. The slower decrease …

Kentucky Warbler (Vol. 64, No. 4), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

Evaluation Of Fomesafen For Broadleaf Weed Control, In Soybeans (Glycine Max), Ricardo A. Jimenez

Masters Theses & Specialist Projects

The lack of effective broadleaf weed control represents one of the major factors having detrimental effects on growth and yield of soybeans. Broadleaf weeds are a serious threat to soybean growers in the southeastern United States. A broad range of herbicides is being used in an effort to control broadleaf weeds in soybeans, and research is still being conducted to find new herbicides that can best work for this purpose. This study involved the use of one these herbicides. It was fomesafen, 5-[2-chloro-4-trifluromethyl) phenoxy]-N-(methyl-sulfonyl)-2-nitrobenzamide, which controls a broad spectrum of broadleaf weeds in soybeans.

The experiment was conducted in the …

Sulfur Accumulation In Xylem Tissue Of Forest Trees From Kentucky & Tennessee & Its Relation To Yearly Growth, Jennifer Sharp

Masters Theses & Specialist Projects

The levels of sulfur present in xylem tissue of Quercus alba (white oak) show a statistically significant increase from 1970 to 1983. This increase was found in trees from three different sites in Kentucky and Tennessee. The levels of sulfur present in Tsuga canadensis (hemlock) show a statistically significant increase from 1955 to 1970. Sensitivity, growth response to the tree's environment, decreased in oaks from 1933 to 1968 but increased slightly in hemlocks during the same time period. The data tend to support a direct relationship between increasing sulfur levels and a reduction in growth. Physiological and ecological studies of …

Kentucky Warbler (Vol. 64, No. 3), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

Adolescent Smoking Status And Socioeconomic Dependence On The Tobacco Crop In Southeastern Kentucky, Gregory Colwell

Masters Theses & Specialist Projects

The purpose of the study was to quantify the relationship between adolescent smoking and economic dependence on the tobacco crop. A survey of 1322 students from a random sample of all secondary schools in four counties in southeastern Kentucky was performed. The survey gathered information concerning smoking behavior and social variables. Chi-square analysis was performed to assess the relationships between these variables in students from families who worked with tobacco and families who did not.

Chi square analysis of female adolescent smoking behavior revealed a significant association between the instances of adolescent smoking and age, friend smoking status and sibling …

Ua66/6/2 Bionews From The Hill, Wku Biology

WKU Administration Documents

Newsletter created by and about the WKU Biology department highlighting students, faculty and alumni activities.

Ua11/2 Wku Agriculture Department Wins Award, Wku Public Affairs

WKU Administration Documents

Press release regarding WKU Agriculture Department award.

Patterns Of Sulfur Deposition In The Wood Of Pinus Echinatia Mill. From The Cumberland Plateau In Kentucky, Darrell Ray

Masters Theses & Specialist Projects

Air pollution and acid precipitation have long had an influence on plant and animal life. In this study, the level of sulfur in the stem tissue of Pinus echinata Mill. was measured in trees from various sites along the western edge of the Cumberland Plateau to determine the presence and pattern of sulfur deposition in this species. Overall, a 40.28% increase in sulfur levels was observed from four sites along the plateau with levels increasing from 0.072 mgS/gdwt in the 1962-66 growth increment to 0.101 mg in the 1982-86 increment. According to a computer generated analysis of variance, the differences …

Response Of Three Cultivars Of Bell Pepper (Capsicum Annuum L.) To Mulching & Irrigation, Cesar Gonzalez

Masters Theses & Specialist Projects

The effect of black polyethylene mulching and drip irrigation on the yield and quality of three cultivars of bell pepper was studied during the 1987 growing season. This study was conducted on a Pembroke silt loam soil on the Western Kentucky University Farm in Bowling Green, Kentucky. The experimental design was a split-plot arranged in randomized complete blocks in four replications with treatment as the main plot and cultivars as the sub-plot.

'California Wonder,' 'Lady Bell F₁ Hybrid: and' Keystone Resistant Giant' cultivars of bell pepper were established in the field during the last week of May. The cultivars …