Life Sciences Commons^™

Kentucky Warbler (Vol. 69, No. 2), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

A Comparison Of Hemoglobin Zurich Erythrocytic Membrane Proteins & Identification Of The Mutation At The Dna Level, Yijuan Liu

Masters Theses & Specialist Projects

Hemoglobin Zurich is a rare blood condition which causes severe rupturing of erythrocytes upon the administration of sulfonamides. Hemoglobin Zurich is caused by a specific genetic mutation in which there is a single amino acid (histidine) substituted for arginine at position 63 in the beta chain of hemoglobin molecules [β 63 Histidine --> Arginine].

In this study, the membrane proteins of Hemoglobin Zurich erythrocytes were compared to membrane proteins from normal human erythrocytes employing sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) and visualized by periodic-acid Schiff (PAS) and DZANDU staining. No differences were detected employing these techniques.

DNA was isolated from …

Cryptococcus Neoformans Serotype Groups Found In Clinical And Environmental Isolates, John Clauson

Masters Theses & Specialist Projects

Cryptococcus neoformans is an encapsulated yeast responsible for severe meningoencephalitis. The importance of epidemiological studies on cryptococcosis has increased since the beginning of the AIDS epidemic. C. neoformans exists in two varieties containing four serotypes, C. neoformans var. neoformans (serotypes A and D) and C. neoformans var. gattii (serotypes B and C). Locally C. neoformans var. neoformans has been associated with pigeon feces during those months having an average temperature of 64.2°F j(17.8°C) and above. Clinical and environmental isolates of C. neoformans obtained from regional hospitals and environmental samplings, respectively, have been grouped into their variety status utilizing canavanine-glycine-bromthymol blue …

Interview With Robert Howard "Bob" Fleming (Fa 388), Manuscripts & Folklife Archives

Folklife Archives Oral Histories

Transcription of interview with Robert Howard Fleming conducted by Kevin Ming on 11 April 1993. From folk studies student project concerning the lifeways of tenant farmers in Logan County, Kentucky.

Interview With Robert Howard "Bob" Fleming (Fa 388), Manuscripts & Folklife Archives

Folklife Archives Oral Histories

Transcription of interview with Robert Howard Fleming conducted by Scott Elrod and Kevin Ming on 2 April 1993. From folk studies student project concerning the lifeways of tenant farmers in Logan County, Kentucky.

Kentucky Warbler (Vol. 69, No. 1), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

Ua12/12 Your Healthstyle, Wku Wellness Center

WKU Administration Documents

Newsletter created by the Wellness Center regarding services, health and fitness.

Interview With Tommy Paschall Regarding Dark Fire Tobacco Barns And Processing (Fa 476), Manuscripts & Folklife Archives

Folklife Archives Oral Histories

Transcription of an interview with Tommy Paschall conducted by John Morgan for an oral history project about dark fire tobacco barns. Paschall discusses tobacco cultivation, processing, and trade. Paschall lived near Hazel, a small town in Calloway County, Kentucky.

Ua66/14/4 Kentucky Partnership Newsletter, Vol. 1, No. 1, Kentucky Partnership For Farm Family Health & Safety

University Organizations

Newsletter created by and about the Kentucky Partnership for Farm Family Health & Safety.

Bioremediation: A New Technology Using Inhabitants Of The Soil, Matthew Fields

Mahurin Honors College Capstone Experience/Thesis Projects

Bioremediation is the use of microorganisms to clean the environment contaminated with various pollutants such as chemical wastes and petroleum -based products. The microbes use the pollutants as their carbon source and by doing so the contaminants are broken down. This paper examined the numbers of indigenous microorganisms in soil contaminated with diesel fuel and oil. Contaminant-degrading microbes were isolated and enumerated using phenanthrene in mineral media. The breakdown of phenanthrene was used as an indicator for contaminant degradation. The effects of aeration and the addition of nutrients on bioremediation processes was also studied. Soil samples used in the performed …

Ua35/11 Student Honors Research Bulletin, Wku Honors Program

WKU Administration Documents

The WKU Student Honors Research Bulletin is dedicated to scholarly involvement and student research. These papers are representative of work done by students from throughout the university.

• Anderson, R. Scott. Anglo-Italian Relations During the Unification of Italy, 1859-1860
• Clark, Anne. Can Extra Fat Make Healthier Hogs? The Effects of Dietary alpha-Linolenic Acid on Antibody Production in Swine Following Stimulation by Red Blood Cells and by Pasteurella Multocida (Serotype A)
• Gottfried, Victoria. Attention Deficit Hyperactivity Disorder: A New Look at an Old Problem
• Halbert, Christy. The Stereotype of the Female Athlete
• Hannah, Kathleen. Innocence and Corruption in Nathaniel Hawthorne's The Scarlet …

Early Behavior Formation In Larval Fathead Minnows, Pimephales Promelas, Shamali Salgado

Masters Theses & Specialist Projects

This study was undertaken in order to identify the approximate times of recruitment of the senses of vision, mechanoreception and chemoreception into the feeding behavior of the fathead minnow, Pimephales promelas, during the first 15 days of life. Larvae were reared on diets of live or freshly killed brine shrimp nauplii and their feeding activity observed daily under light and dark conditions. On every third day during the feeding trials, the larvae were reverse-fed the type of food upon which they were reared. The first evidence of feeding occurred on Day 3, when larvae conditioned on dead food ingested …

Kentucky Warbler (Vol. 68, No. 4), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

Ua12/12 Your Healthstyle, Wku Wellness Center

WKU Administration Documents

Newsletter created by the Wellness Center regarding services, health and fitness.

Interview With Tommy Paschall Regarding Dark Fire Tobacco Barns And Processing (Fa 476), Manuscripts & Folklife Archives

Folklife Archives Oral Histories

Transcription of an interview with Tommy Paschall conducted by John Morgan for an oral history project about dark fire tobacco barns. Paschall discusses tobacco cultivation and the building of dark fire tobacco barns in western Kentucky, specifically Calloway County, Kentucky.

Kentucky Warbler (Vol. 68, No. 3), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

A Study Of Kentucky’S Agricultural Performance Using Shift-Share Analysts, David Neal Cundiff

Masters Theses & Specialist Projects

In this paper the theory that Kentucky has a comparative advantage in agricultural employment, when compared to the United States, is examined. In order to test this hypothesis, a dynamic shift-share analysis was conducted using the thirteen major economic sector of Kentucky over the period 1970 to 1989. The resulting regional shift components, or competitive components, give support to the theory that a comparative advantage for Kentucky in agriculture does exist. Annual regional shift components, as well as their dynamic counterpart, possess predominately positive values, indicating outperformance by Kentucky’s agricultural sector when compared to the United States economy as a …

Repeatabilities Of Pelvic Area Measurements Between And Within Technicians With Varying Levels Of Experience, Steven Ftizner

Masters Theses & Specialist Projects

There has been some concern recently about the ability of technicians to accurately measure and rank prospective replacement heifers and herd bulls for pelvic area. Two studies were conducted to determine the repeatabilities between and within technicians with varying levels of experience in measuring pelvic areas.

In both studies, virgin yearling heifers were measured for pelvic height and width to determine pelvic area. In each study, technicians included a veterinarian with several years experience in reproductive physiology practice and Western Kentucky University students with varying levels of experience in rectal examinations. In the first study , the veterinarian used both …

Ua66/6/2 Bionews From The Hill, Wku Biology

WKU Administration Documents

Newsletter created by and about WKU Biology highlighting the activities of faculty, students and alumni.

The Ratio Of Heinz Body Formation In Different Hemoglobin Zurich Subjects, Yenya Hu

Masters Theses & Specialist Projects

Hemoglobin Zurich is a hemoglobin anomaly that results when one amino acid (histidine) is substituted by arginine at position 63 in the beta chain of hemoglobin molecules [β 63 His—Arg]. When Hemoglobin Zurich individuals are exposed to sulfonamide medication, their hemoglobins denature and subsequently form Heinz bodies which attach to the surface of the plasma membrane.

Four Hemoglobin Zurich family members were the subjects of the current study. They included a splenectomized female subject, non-splenectomized female and male subjects, and a non-splenectomized female member without Hemoglobin Zurich as the control. The results collaborate that splenectomy increases the number of erythrocytes …

Kentucky Warbler (Vol. 68, No. 2), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

A Modified Scheme For The Isolation And Enumeration Of Bacteria In Municipal Sewage Sludge, Kelly Ball

Masters Theses & Specialist Projects

Because of the potential health hazards associated with the use of sludge for agricultural purposes, Dudley et al (1980) published a scheme for the routine analysis of bacteria in municipal sewage sludge. In this study, the Dudley et al scheme (1980) was modified by updating some of the procedures. Aerobically digested sludge generated by the Bowling Green Wastewater Treatment Plant, Bowling Green, Kentucky, was analyzed using the modified scheme. Sludge samples were collected once every two months over a one-year period from October 1989 to August 1990.

Egg yolk-free tryptose sulfite cycloserine agar in conjunction with the revewrse CAMP test …

Swine Breed Differences In Agglutination Titers Following Vaccination With Sheep Red Blood Cells And Pasteurella Multocida (Serotype A), Kenneth Stalder

Masters Theses & Specialist Projects

An investigation into the genetic differences in the humoral immune response of swine following vaccination with a sheep red blood cell solution (SRBC) and a commercially prepared Pasteurella multocida (serotype A) bacterin (PmA) was conducted on a total of 268 pigs from two individual trials. This study was also conducted to evaluate the humoral immune response of pigs to a non-pathogen (SPEC) and a known pathogen to swine (PmA). The pigs used in the first trial were from 22 litters born between January 1991 and July 1991. The pigs consisted of Hampshire x Yorkshire (n=114), purebred Yorkshire (n=70) and Hampshire …

Can Extra Fat Make Healthier Hogs?, Anne Elise Clark

Mahurin Honors College Capstone Experience/Thesis Projects

Based on research by Fritsche (1991), Kelley (1988), and Caruso and Jeska (1990), it has been postulated that the addition of omega-3 fatty acids to the diet enhances the immune response of several species. This study was an attempt to ascertain the effects of addition of dietary omega-3 fatty acids in the form of ground flax contained in Omega Gold on the immune response of swine. The goal of this study was twofold: to determine the effects of ground flax on the humoral immunity of young pigs, and to differentiate between effects gained from enhancement of passive and active immunity. …

Ua68/6/1 Broomsedge Chronicles: Farm Living In South Central Kentucky, Wku English, Wku Glasgow

WKU Administration Documents

A collection of essays written by English 100 Freshman Composition and English 200 Introduction to Literature students attending WKU Glasgow from 1983 through 1992 taught by Loretta Murrey. Student authors are: Joyce Alford, Joyce Amer, Jeff Ballard, Sandie Barrick, Jerry Bean, Shela Bingham, Brent Bledsoe, Steven Bunch, Billy Carver, Angela Cowan, Karen Decker, Betty Dillahay, Dibbie Dilley, Amy Doyel, Jeff Duncan, Craig Emmitt, Barbara England, Kathy Fancher, Amanda Gillon, Michelle Glover, Jeanelle Gooch, Faye Johnson, Celena Martin, Sonia Martin, Tracy Mathews, Ila Moody, Angela Morris, William Myatt, Judy Parker, Dorothean Powell, Maria Pulanco, Diane Rather, Jennifer Reneau, LaDarra Rich, Pam …

Thermal Properties Of Some Lipid Components Of Cell Membranes, Lihua Wei

Masters Theses & Specialist Projects

Phospholipids are the major structural components of the lipid portion of biological membranes. The study of the thermal properties of these phospholipid provides a systematic understanding of the relationships between the chemical structures and functional properties of phospholipid molecules in artificial and biological membranes. This study examined the thermal behavior of phospholipids in the solid state using DSC and TG coupled with FITR. The phospholipids were found to have phase transitions below the melting point. Egg-yolk lecithin had four transitions below its melting point; sphingomyelin had two phase transitions below its melting point. Phosphatidylethanolamine and phosphatidylserine had complex phase transitions. …

Kentucky Warbler (Vol. 68, No. 1), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

Ua1d Thomas Coohill Personnel File, Wku Human Resources

WKU Administration Documents

Personnel file of Biology professor Thomas Coohill.

Evidence For The Location Of Aviral Protease & Protease Cleavage Sites In The 105 Kilodalton Protein Of Southern Bean Mosaic Virus, Timothy Shannon

Masters Theses & Specialist Projects

Southern Bean Mosaic Virus (SBMV) codes for a large polyprotein which is subsequently cleaved into smaller functional proteins. A virally encoded protease is suspected of mediating the cleavages. SBMV, picornaviruses and picornavirus-like viruses appear to have similar genomic organizations, which would place the protease region of the genome between the VPg and RNA dependen,, RNA polymerase regions. Protein sequence comparisons revealed homology between SBMV putative protease and the known proteases from foot and mouth disease virus, encephlamyocarditis virus, poliovirus, and cowpea mosaic virus. Experimental evidence provides little information on the exact location of the protease; however, protein sequence analysis suggests …

Creation Of A Vector For Regulated Expression Of Antisense Rna In Escherichia Coli, Wen-Jun Wu

Masters Theses & Specialist Projects

A plasmid (pGEM-SD) was constructed in which the Shine-Dalgarno sequence had been deleted from pGEM-7zf(+) plasmid to generate a vector for regulated expression of antisense RNA. The binding of antisense RNA to mRNA provides a potent mechanism by which specific transcripts can be translationally inactivated. Although part of the lac operator sequence was deleted in pGEM-SD plasmid, it was proven that mRNA still can be induced under the control of lac promoter. Recombinant plasmids were generated by ligating bacterial genornic DNA into pGEM-SD plasmid, but the orientation of the inserted gene with respect to the lac promoter has not been …