Life Sciences Commons^™

Signs Of Mood And Anxiety Disorders In Chimpanzees, Hope Ferdowsian, Debra Durham, Charles Kimwele, Godelieve Kranendonk, Emily Otali, Timothy Akugizibwe, J. B. Mulcahy, Lilly Ajarova, Cassie Meré Johnson

Sentience Collection

Background: In humans, traumatic experiences are sometimes followed by psychiatric disorders. In chimpanzees, studies have demonstrated an association between traumatic events and the emergence of behavioral disturbances resembling posttraumatic stress disorder (PTSD) and depression. We addressed the following central question: Do chimpanzees develop posttraumatic symptoms, in the form of abnormal behaviors, which cluster into syndromes similar to those described in human mood and anxiety disorders?

Methodology/Principal Findings: In phase 1 of this study, we accessed case reports of chimpanzees who had been reportedly subjected to traumatic events, such as maternal separation, social isolation, experimentation, or similar experiences. We applied and …

Self-Harm In Laboratory-Housed Primates: Where Is The Evidence That The Animal Welfare Act Amendment Has Worked?, Jonathan Balcombe, Hope Ferdowsian, Debra Durham

Animal Welfare Collection

The 1985 amendment to the United States Animal Welfare Act (AWA) to promote psychological well being of primates in the laboratory represents an acknowledgment of an important welfare problem concerning nonhuman animals. How effective has this amendment been? Perhaps the best-known contributor to psychological distress in primates in the laboratory is nonsocial housing; yet, available analyses suggest that little progress has been made in avoiding single-caging of these animals. Another way to assess psychological well being is to examine rates of self-abusive behavior in laboratory primates. If the AWA has been effective, then post-AWA self-harm rates might be lower than …

Microdialect And Group Signature In The Song Of The Skylark Alauda Arvensis, Elodie Briefer, Fanny Rybak, Thierry Aubin

Sentience Collection

The Skylark Alauda arvensis is a territorial species of open landscape in which pairs settle in stable and adjacent territories during the breeding season. Due to the heterogeneity of the habitat, territories are gathered in patches spaced by a few kilometres, in which each male produces very long and complex flight songs as a part of the territorial behaviour. We showed that, in a given patch, all the males (neighbours) share some particular sequences of syllables in their songs, whereas males settled in different patches (strangers) have almost no sequences in common. Such a phenomenon is known as microdialect. To …

Humane Cat Population Management Guidance, International Companion Animal Management Coalition

Stray and Feral Animal Populations Collection

The International Companion Animal Management Coalition has produced this document to provide government bodies and non-governmental organisations with a detailed resource to support them in their development and implementation of effective and humane programmes to manage cat populations.

Population Survey (Census) Of Stray Dog Population In Ahmedabad, Gujarat, Rahul Sehgal, Sunil Chawla, Jack Reece

Stray and Feral Animal Populations Collection

Ahmadabad, the biggest city of Gujarat with a human population of approximately, 6 million. The rapidly expanding population of Ahmadabad means that the metropolitan region extends far outside from the confines of the old city. However, the development is rapid but uncontrolled. Therefore, there are a large number of areas that are prone to stray dogs and these areas are constantly increasing. The old methods of population control by strychnine poisoning or electrocution employed by the municipal corporations have not proved effective in controlling street dog populations, as new dogs soon migrate into the areas, where the local dog population …

Haemato-Biochemical Changes In Natural Cases Of Canine Babesiosis, Showkat Ahmad Shah, Naresh Kumar Sood, Srinivasa Rao Tumati

Biochemistry Collection

Incidence of severe anemia due to babesiosis in dogs resulting in death in a short time of illness is on the increase in Punjab, during last few decades due to introduction of exotic breeds like Grey Hound, German shepherd, Doberman, Labrador and others. In the present study the hematological and biochemical changes in blood samples obtained from 4 dogs naturally infected with Babesia were evaluated. The dogs were presented to the Department of Veterinary Clinical Services Complex, GADVASU, Ludhiana from August 2008 to April 2009. The evaluation included Hemoglobin (Hb), Red Blood Cell count (RBC), Packed Cell Volume (PCV), Mean …

Biochemical Methane Potential Of Different Organic Wastes And Energy Crops From Estonia, M. Luna-Derisco, A. Normak, K. Orupõld

Biochemistry Collection

The biochemical methane potential (BMP) of different Estonian substrates as alternative sources for biogas production was studied. For this purpose, the BMP test was carried out in batch mode at mesophilic temperature (36°C). Substrates were divided into 2 groups: agricultural substrates (silage, hay, cattle and pig slurry) and food industry residues (milk, brewery and cereal industry residues). Methane yields obtained were between 286–319 L kgVS^-1 for silage and hay, 238–317 L kgVS^-1 for animal slurry and 272–714 L kgVS^-1 for agro-industrial wastes. The highest methane yield was obtained from sour cream (714 L kgVS^-1), the …

Effect Of Dietary Antibacterial Peptide And Zinc-Methionine On Performance And Serum Biochemical Parameters In Piglets, J. H. Wang, C. C. Wu, J. Feng

Biochemistry Collection

The objective of this study was to evaluate the effect of dietary supplementation of antibacterial peptide and zinc methionine on performance and some serum biochemical parameters of weaned piglets. Rongchang male piglets (28 days of age, initial weight 8.4 ± 0.65 kg) were used. All piglets were randomly allotted to four diets including the control group, the antibacterial peptide (AP) group containing 10 mg antibacterial peptide/kg diet, the zinc methionine (Zn-Met) group with 1 200 zinc methionine/kg diet (equal to 200 mg Zn²⁺/kg diet), the zinc methionine/antibacterial peptide (Zn/AP) group containing 10 mg antibacterial peptide and 1 200 …

A Guide To Defining And Implementing Protocols For The Welfare Assessment Of Laboratory Animals: Eleventh Report Of The Bvaawf/Frame/Rspca/Ufaw Joint Working Group On Refinement, P. Hawkins, D. B. Morton, O. Burman, N. Dennison, P. Honess, M. Jennings, S. Lane, V. Middleton, J. V. Roughan, S. Wells, K. Westwood

Research Methodology and Laboratory Animals Collection

The refinement of husbandry and procedures to reduce animal suffering and improve welfare is an essential component of humane science. Successful refinement depends upon the ability to assess animal welfare effectively, and detect any signs of pain or distress as rapidly as possible, so that any suffering can be alleviated. This document provides practical guidance on setting up and operating effective protocols for the welfare assessment of animals used in research and testing. It sets out general principles for more objective observation of animals, recognizing and assessing indicators of pain or distress and tailoring these to individual projects. Systems for …

The Canine Sand Maze: An Appetitive Spatial Memory Paradigm Sensitive To Age-Related Change In Dogs, Hannah Salvin, Paul D. Mcgreevy, Perminder S. Sachdev, Michael Valenzuela

Spatial Cognition Collection

Aged dogs exhibit a spectrum of cognitive abilities including a syndrome similar to Alzheimer's disease. A major impediment to research so far has been the lack of a quick and accurate test of visuospatial memory appropriate for community-based animals. We therefore report on the development and validation of the Canine Sand Maze. A 4.5-m-diameter circular pool was filled with a sand and powdered food reward mix to a depth of 10 cm. Dogs were given 4 habituation and 16 learning trials which alternated a food reward being half (control trials) or fully-buried (acquisition trials) in a fixed location. After a …

An Assessment Of The Use Of Chimpanzees In Hepatitis C Research Past, Present And Future: 2. Alternative Replacement Methods, Jarrod Bailey

Experimentation Collection

The use of chimpanzees in hepatitis C virus (HCV) research was examined in the report associated with this paper (1: Validity of the Chimpanzee Model), in which it was concluded that claims of past necessity of chimpanzee use were exaggerated, and that claims of current and future indispensability were unjustifiable. Furthermore, given the serious scientific and ethical issues surrounding chimpanzee experimentation, it was proposed that it must now be considered redundant — particularly in light of the demonstrable contribution of alternative methods to past and current scientific progress, and the future promise that these methods hold. This paper builds on …

An Assessment Of The Use Of Chimpanzees In Hepatitis C Research Past, Present And Future: 1. Validity Of The Chimpanzee Model, Jarrod Bailey

Experimentation Collection

The USA is the only significant user of chimpanzees in biomedical research in the world, since many countries have banned or limited the practice due to substantial ethical, economic and scientific concerns. Advocates of chimpanzee use cite hepatitis C research as a major reason for its necessity and continuation, in spite of supporting evidence that is scant and often anecdotal. This paper examines the scientific and ethical issues surrounding chimpanzee hepatitis C research, and concludes that claims of the necessity of chimpanzees in historical and future hepatitis C research are exaggerated and unjustifiable, respectively. The chimpanzee model has several major …

Salmon For Terrestrial Protected Areas, Chris T. Darimont, Heather M. Bryan, Stephanie M. Carlson, Morgan D. Hocking, Misty Macduffee, Paul C. Paquet, Michael H. H. Price, Thomas E. Reimchen, John D. Reynolds, Christopher C. Wilmers

Environment and Nature Conservation Collection

Although managers safeguard protected areas for migratory species, little consideration has been given to how migratory species might benefit parks. Additionally, whereas land‐sea connections are considered in management of protected areas, most effort has focused on reducing negative “downstream” processes. Here, we offer a proposal to promote positive “upstream” processes by safeguarding the seasonal pulse of marine nutrients imported into freshwater and riparian ecosystems by spawning migrations of Pacific salmon. Currently, high rates of fishing limit this important contribution to species and processes that terrestrial parks were designed to protect. Accordingly, we propose limiting exploitation in areas and periods through …

Vocal Learning In Grey Parrots: A Brief Review Of Perception, Production, And Cross-Species Comparisons, Irene M. Pepperberg

Sentience Collection

This chapter briefly reviews what is known—and what remains to be understood—about Grey parrot vocal learning. I review Greys’ physical capacities—issues of auditory perception and production—then discuss how these capacities are used in vocal learning and can be recruited for referential communication with humans. I discuss cross-species comparisons where applicable and conclude with a description of recent research that integrates issues of reference, production and perception.

The Effect Of Claw Horn Disruption Lesions And Body Condition Score At Dry-Off On Survivability, Reproductive Performance, And Milk Production In The Subsequent Lactation, V. S. Machado, L. S. Caixeta, J. A. A. Mcart, R. C. Bicalho

Farm Animal Welfare Collection

The objective of this study was to evaluate the effects of claw horn disruption lesions (CHDL; sole ulcers and white line disease) and body condition score (BCS) at dry-off on survivability, milk production, and reproductive performance during the subsequent lactation. An observational prospective cohort study was conducted on a large commercial dairy in Cayuga County, New York, from September 2008 until January 2009. A total of 573 cows enrolled at dry-off were scored for body condition and hoof trimmed; digits were visually inspected for the presence of CHDL. The BCS data were recategorized into a 3-level variable BCS group (BCSG), …

Are Unfamiliar Neighbours Considered To Be Dear-Enemies?, Elodie Briefer, Fanny Rybak, Thierry Aubin

Sentience Collection

Background: Discriminating threatening individuals from non-threatening ones allow territory owners to modulate their territorial responses according to the threat posed by each intruder. This ability reduces costs associated with territorial defence. Reduced aggression towards familiar adjacent neighbours, termed the dear-enemy effect, has been shown in numerous species. An important question that has never been investigated is whether territory owners perceive distant neighbours established in the same group as strangers because of their unfamiliarity, or as dear-enemies because of their group membership.

Methodology/Principal Findings: To investigate this question, we played back to male skylarks (Alauda arvensis) songs of adjacent neighbours, distant …

Capuchins (Cebus Apella) Can Solve A Means-End Problem, Anna M. Yocom, Sarah T. Boysen

Sentience Collection

Three capuchin monkeys (Cebus apella) were tested on a 2-choice discrimination task designed to examine their knowledge of support, modeled after Hauser, Kralik, and Botto-Mahan’s (1999) experiments with tamarins. This task involved a choice between 2 pieces of cloth, including 1 with a food reward placed on its surface, and a second cloth with the food reward next to its surface. After reliably solving the basic problem, the capuchins were tested with various alternations of the original food reward and cloth. The capuchins were able to solve the initial task quickly, and generalize their knowledge to additional functional and nonfunctional …

Human Induced Rotation And Reorganization Of The Brain Of Domestic Dogs, Taryn Roberts, Paul Mcgreevy, Michael Valenzuela

Morphology Collection

Domestic dogs exhibit an extraordinary degree of morphological diversity. Such breed-to-breed variability applies equally to the canine skull, however little is known about whether this translates to systematic differences in cerebral organization. By looking at the paramedian sagittal magnetic resonance image slice of canine brains across a range of animals with different skull shapes (N = 13), we found that the relative reduction in skull length compared to width (measured by Cephalic Index) was significantly correlated to a progressive ventral pitching of the primary longitudinal brain axis (r = 0.83), as well as with a ventral shift in the position …

Dairy Farmer Attitudes And Empathy Toward Animals Are Associated With Animal Welfare Indicators, Camilla Kielland, Eystein Skjerve, Olav Østerås, Adroaldo José Zanella

Societal Attitudes Toward Animals Collection

Attitudes and empathy of farmers influence human–animal interaction, thereby affecting their behavior toward animals. The goal was to investigate how measures of attitude and empathy toward animals were associated with animal welfare indicators such as milk yield, mastitis incidence, fertility index, and the prevalence of skin lesions on cows. To assess empathy toward animals, a photo-based pain assessment instrument was developed depicting various conditions that could be associated with some degree of pain in cattle and included questions aimed at assessing attitudes toward animals. Photos of painful conditions are useful in eliciting measurable empathic responses to pain in humans. A …

The Vision Of Toxicity Testing In The 21st Century: Moving From Discussion To Action, Melvin E. Andersen, Daniel Krewski

Toxicology and Animal Models in Research Collection

Over the past year, a series on commentaries have appeared in the Toxicological Sciences Forum Series related to the 2007 National Research Council (NRC) publication, Toxicity Testing in the 21st Century: A Vision and A Strategy. The first article in the series provided an overview of the vision and was accompanied by an editorial by the three editors of Toxicological Sciences. During the past year, eight invited commentaries from the academic, industrial, and regulatory sectors have provided diverse perspectives on the vision, noted challenges to its implementation, and highlighted aspects of toxicity testing that were not addressed in …

On-Farm Welfare Assessment For Regulatory Purposes: Issues And Possible Solutions, Jan Tind Sørensen, David Fraser

Assessment of Animal Welfare Collection

On-farm welfare assessment has been used mainly for non-regulatory purposes such as producer education or to qualify for voluntary welfare-assurance programs. The application of on-farm assessments in regulatory programs would require four issues to be addressed: (1) selecting criteria that are widely accepted as valid by diverse citizens, (2) setting minimum legal levels, (3) achieving the high level of fairness and objectivity required for legally binding requirements, and (4) achieving the cost-efficiency needed for widespread use of the methods. Issues 1 and 2 pose a particular problem because different citizens disagree on what they understand as good animal welfare, with …

Cross-Sector Review Of Drivers And Available 3rs Approaches For Acute Systemic Toxicity Testing, Troy Seidle, Sally Robinson, Tom Holmes, Stuart Creton, Pilar Prieto, Julia Scheel, Magda Chlebus

Experimentation Collection

Acute systemic toxicity studies are carried out in many sectors in which synthetic chemicals are manufactured or used and are among the most criticized of all toxicology tests on both scientific and ethical grounds. A review of the drivers for acute toxicity testing within the pharmaceutical industry led to a paradigm shift whereby in vivo acute toxicity data are no longer routinely required in advance of human clinical trials. Based on this experience, the following review was undertaken to identify (1) regulatory and scientific drivers for acute toxicity testing in other industrial sectors, (2) activities aimed at replacing, reducing, or …

Short-Beaked Common Dolphin (Delphinus Delphis) Occurrence In The Moray Firth, North-East Scotland, Kevin P. Robinson, Sonja Eisfeld, Marina Costa, Mark P. Simmonds

Ecology Collection

The short-beaked common dolphin (Delphinus delphis) is regarded as notably rare or absent from the northern North Sea, but recent evidence suggests a rising frequency of the species in these waters with increasing regional sea temperatures. The following paper documents the presence of D. delphis in the Moray Firth in north-east Scotland and provides the first evidence for the sustained occurrence of these delphinids in this region during the warmer summer months at least. Sightings were collated during systematic surveys of the outer Moray Firth between 2001 and 2009 by independent research teams from the CRRU and WDCS. A total …

Toward A Synthesis Of Conservation And Animal Welfare Science, David Fraser

Conservation Biology and Animal Welfare Collection

Conservation biology and animal welfare science are multidisciplinary fields of research that address social concerns about animals. Conservation biology focuses on wild animals, works at the level of populations, ecological systems and genetic types, and deals with threats to biodiversity and ecological integrity. Animal welfare science typically focuses on captive (often domestic) animals, works at the level of individuals and groups, and deals with threats to the animals’ health and quality of life. However, there are many areas of existing or potential overlap: (i) many real-life problems, such as environmental contamination, urban development and transportation, create problems for animals that …

Calling On Science: Making “Alternatives” The New Gold Standard, Melvin E. Andersen

in Vitro Research Models Collection

All of life’s great journeys start with a goal in mind! The 2007 NAS report, Toxicity Testing in the 21st Century – A Vision and A Strategy, has proposed a clear goal. This report envisions a not-so-distant future where all routine toxicity testing for environmental agents will be conducted in human cells in vitro evaluating perturbations of cellular responses in a suite of toxicity pathway assays. Dose response modeling would utilize computational systems biology models of the circuitry underlying each toxicity pathway; in vitro to in vivo extrapolations would use pharmacokinetic models, ideally physiologically based pharmacokinetic models, to predict human …

Your Attention Please: Increasing Ambient Noise Levels Elicits A Change In Communication Behaviour In Humpback Whales (Megaptera Novaeangliae), Rebecca A. Dunlop, Douglas H. Cato, Michael J. Noad

Bioacoustics Collection

High background noise is an important obstacle in successful signal detection and perception of an intended acoustic signal. To overcome this problem, many animals modify their acoustic signal by increasing the repetition rate, duration, amplitude or frequency range of the signal. An alternative method to ensure successful signal reception, yet to be tested in animals, involves the use of two different types of signal, where one signal type may enhance the other in periods of high background noise. Humpback whale communication signals comprise two different types: vocal signals, and surface-generated signals such as ‘breaching’ or ‘pectoral slapping’. We found that …

A Modular One-Generation Reproduction Study As A Flexible Testing System For Regulatory Safety Assessment, Richard Vogel, Troy Seidle, Horst Spielmann

Experimentation Collection

The European Union’s Registration, Evaluation and Authorisation of Chemicals (REACH) legislation mandates testing and evaluation of approximately 30,000 existing substances within a short period of time, beginning with the most widely used “high production volume” (HPV) chemicals. REACH testing requirements for the roughly 3000 HPV chemicals specify three separate tests for reproductive toxicity: two developmental toxicity studies on different animal species (OECD Test Guideline 414) and a two-generation reproduction toxicity study (OECD TG 416). These studies are highly costly in both economic and animal welfare terms. OECD TG 416 is a fertility study intended to evaluate reproductive performance of animals …

Can Animal Models Of Disease Reliably Inform Human Studies?, H. Bart Van Der Worp, David W. Howells, Emily Sena, Michelle J. Porritt, Sarah Rewell, Victoria O'Collins, Malcolm Macleod

Validation of Animal Experimentation Collection

• The value of animal experiments for predicting the effectiveness of treatment strategies in clinical trials has remained controversial, mainly because of a recurrent failure of interventions apparently promising in animal models to translate to the clinic.
• Translational failure may be explained in part by methodological flaws in animal studies, leading to systematic bias and thereby to inadequate data and incorrect conclusions about efficacy.
• Failures also result because of critical disparities, usually disease specific, between the animal models and the clinical trials testing the treatment strategy.
• Systematic review and meta-analysis of animal studies may aid in the selection of the most …

Publication Bias In Reports Of Animal Stroke Studies Leads To Major Overstatement Of Efficacy, Emily Sena, H. Bart Van Der Worp, Philip M.W. Bath, David W. Howells, Malcolm Macleod

Validation of Animal Experimentation Collection

The consolidation of scientific knowledge proceeds through the interpretation and then distillation of data presented in research reports, first in review articles and then in textbooks and undergraduate courses, until truths become accepted as such both amongst “experts” and in the public understanding. Where data are collected but remain unpublished, they cannot contribute to this distillation of knowledge. If these unpublished data differ substantially from published work, conclusions may not reflect adequately the underlying biological effects being described. The existence and any impact of such “publication bias” in the laboratory sciences have not been described. Using the CAMARADES (Collaborative Approach …

Non-Invasive Genetic Sampling Of Faecal Material And Hair From The Grey-Headed Flying-Fox (Pteropus Poliocephalus), Heather J. Baldwin, Stephen J. Hoggard, Stephanie T. Snoyman, Adam J. Snow, Culum Brown

Veterinary Science and Medicine Collection

Remote-sampling DNA from animals offers obvious benefits for species that are difficult to sample directly and is less disruptive for species of conservation concern. Here we report the results of a pilot study investigating non-invasive DNA sampling of the grey-headed flying-fox (Pteropus poliocephalus), a threatened species that is restricted to the east coast of Australia. We successfully extracted DNA from fresh scats and hair, each of which was of sufficient quality for amplifying mitochondrial DNA markers and microsatellites. A single-locus multitube approach was used to investigate amplification success and genotyping reliability. Faecal samples yielded a higher proportion of successful amplifications …