Life Sciences Commons^™

Antimicrobial Peptides In Transgenic Silkworm Silk, David Jaden Turner

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Overview: People in India and China have produced silk textiles for thousands of years. Silk is a biodegradable, biocompatible compound used in the production of clothing, bedding, furniture, industrial materials, and medical applications. Over the last 30 years, research has increasingly investigated silk’s antimicrobial effects and how to augment its natural abilities. Antimicrobial peptides, or AMPs, are also an area of increasing interest as the rise of antibiotic resistance reduces the efficacy of current treatments. This project plans to systematically synthesize a fusion protein that incorporates the beneficial properties of each constituent into commercial silkworms.

Innovation: This project seeks to …

Multispecies Genomic Sex Identification Using Ddx3 Gene Polymorphisms, Jessica Felts

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

PCR sex determination assays must be reliable and cost effective due to the frequent and integral use of these assays in biological research and the animal production industry. Thus, the design of proof of a primer pair with a built-in control is warranted to not only bypass the extra cost of a multiplex reaction, but also to prevent anomalous results that have been documented with other primer pairs.

The objective of this study was to design primer pairs with built in PCR amplification control to identify sex in Equus caballus (domestic horse), Homo sapiens (humans), Macaca mulatta (rhesus macaque), and …

Investigating Mitochondrial Influence On The Rate Of Anaerobic Glycolysis In An In Vitro Model, Mackenzie Jenna' Taylor

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The rate at which the muscle acidifies after an animal is harvested has a profound effect on the quality of the resulting pork. When acidification increases gradually, desirable pork quality characteristics are developed. In contrast, rapid acidification deteriorates pork quality, exemplified by the pale, soft, and exudative (PSE) pork defect. The rate of acidification is determined by the rate of anaerobic metabolism in postmortem muscle. Yet the processes controlling postmortem anaerobic metabolism are not well understood. Recent research suggests that mitochondria, the powerhouse of the cell, may influence this process by competing for substrate (pyruvate) with anaerobic metabolism, thereby reducing …

Water Conservation Through Drought-Resilient Landscape Plants And Deficit Irrigation, Ji-Jhong Chen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Increases in urban population and inadequate rainfall result in imbalanced water budgets in urban and sub-urban regions. Water conservation becomes important in urban landscapes because of increased water demands. Modern landscape designs require drought-resistant plants to maintain urban landscape greenness during water scarcity. Irrigating plants at their irrigation requirements, which is the minimum irrigation rate that can achieve acceptable aesthetic quality, can also conserve water. However, the drought tolerance and irrigation requrements of most landscape plants have not been widely evaluated. Shepherdia ×utahensis ‘Torrey’ (‘Torrey’ buffaloberry) and Penstemon species (beardtongues) are low-water-use landscape plants, but their drought resistance mechanisms are …

Anthropogenic Factors Affecting Common Raven Occurrence And Depredation Of Artificial Nests Within Greater Sage‐Grouse Habitat In Southern Utah, Zoë S. Moffett

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Certain species of wildlife are more generalist and adaptive than others. These species often flourish when supported by human activities that provide additional food and habitat for them. The common raven (Corvus corax; hereafter raven) is one such species; their populations have risen and spread throughout the Intermountain West. As generalist scavengers and predators, ravens have been found to pose a severe threat to several threatened or sensitive species, including the greater sage-grouse (Centrocercus urophasianus; hereafter sage-grouse). The purpose of my research was to determine the specific anthropogenic and habitat factors that may increase the threat …

Physiological Consequences Of Adrenal Enlargement And Implications For Toxin Resistance In North American Snakes, Megen E. Kepas

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The long observed relationship between enlarged adrenal glands and toad-eating in snakes has remained a mystery in physiological ecology and herpetology. It has been predicted that large adrenal glands may be capable of producing higher amounts of key hormones, and that higher plasma hormone levels may lend some behavioral or physiological benefits after a toxic toad has been eaten. I address questions surrounding adrenal enlargement and its potential benefits to toad eaters in four research chapters. In my second chapter, I quantify adrenal hormone output at different embryonic stages in the toad-generalist snake Thamnophis elegans and examine the ability of …

Precipitation And Soil Properties Determine Long-Term Consequences Of Disturbance And Invasion In Drylands, Tyson Terry

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Disturbance and invasive species have dramatic effects on desert plant communities, often resulting in degradation or shifts to alternative plant communities. Climate and soil properties determine water availability to plants, and have been thought to drive patterns of recovery following disturbance and potential for invasion.

In chapter II we used a combination of natural gas pipelines and satellite imagery to understand how recovery from a uniform disturbance differs across precipitation and soil gradients. We used a recovery ratio (disturbed/undisturbed) of pipeline pixels and their undisturbed nearest neighbor pixel to quantify recovery in a comparable way across precipitation gradients. We found …

Agricultural Irrigated Land And Irrigation Water Use In Utah, Burdette Barker, Matt Yost, Cody Zesiger

All Current Publications

Utah is considered the second driest state in the United States. As a result, much of the agriculture and horticulture in Utah depends upon irrigation. Irrigation is an important feature of agriculture in Utah. The distribution of irrigated and water use varies across the state, based on water availability, topography, and soils. In recent years, sprinkler irrigation has overtaken surface methods as the dominant irrigation method, based on irrigated area, in Utah. About 75% to 80% of water withdrawals in Utah are for irrigation, with about 68% of that water being consumptive use. Alfalfa, other hay crops, and pasture are …

Manejo De La Chinche Hedionda Marrón Marmolada Para Frutas Y Hortalizas En Utah, Lori R. Spears, Marion Murray, Diane G. Alston, Celina Wille

All Current Publications

La chinche hedionda marrón marmolada es una plaga molesta y conómicamente importante para la agricultura que invadió Norteamérica desde el este de Asia a fines de la década de 1990.

Beginner's Guide To Common Native Bees, Cody Zesiger, Elizabeth Cohen, Hannah Jarvis, Lori R. Spears, Ricardo Ramirez

All Current Publications

Utah is a superb place for beginning bee enthusiasts. Over 1,000 species of native bees exist in Utah (Cane, 2015). Southern Utah alone has approximately the same number of bee species as the entire eastern U.S. coast. There are many reasons for appreciating bees and encouraging their presence in the landscape. Specifically, bees are key to a sustainable environment as they are essential pollinators of food and fiber crops. Bees can also be appreciated for their striking diversity in color and size. This fact sheet highlights the different bee species that you may see in Utah.

C···O And Si···O Tetrel Bonds: Substituent Effects And Transfer Of The Sif3 Group, Zhihao Niu, Qiaozhuo Wu, Qingzhong Li, Steve Scheiner

Chemistry and Biochemistry Faculty Publications

The tetrel bond (TB) between 1,2-benzisothiazol-3-one-2-TF₃-1,1-dioxide (T = C, Si) and the O atom of pyridine-1-oxide (PO) and its derivatives (PO-X, X = H, NO₂, CN, F, CH₃, OH, OCH₃, NH₂, and Li) is examined by quantum chemical means. The Si···O TB is quite strong, with interaction energies approaching a maximum of nearly 70 kcal/mol, while the C···O TB is an order of magnitude weaker, with interaction energies between 2.0 and 2.6 kcal/mol. An electron-withdrawing substituent on the Lewis base weakens this TB, while an electron-donating group has the opposite …

Variable Synchrony In Insect Outbreak Cycling Across A Forest Landscape Gradient: Multi-Scale Evidence From Trembling Aspen In Alberta, Barry J. Cooke, Jens Roland

Aspen Bibliography

Using multi-scale trembling aspen tree-ring width data from Alberta, we show that scaling has a profound influence on dendroecological inferencing. At all scales of sampling, there is a significant climatological signal whose strength is nevertheless superseded by the pervasive effect of insect herbivory. At the smallest spatial scale, 20 km x 20 km, we demonstrate a quasi-periodic pattern of sharp growth reductions and the existence of negative spatial correlations among successive outbreaks. At the intermediate spatial scale of 20 km x 80 km, we show that the period 1930-1963 was marked by extremely low correlations in aspen ring widths, with …

Ranchsatdb: A Genome-Wide Simple Sequence Repeat (Ssr) Markers Database Of Livestock Species For Mutant Germplasm Characterization And Improving Farm Animal Health, Naveen Duhan, Simardeep Kaur, Rakesh Kaundal

Plants, Soils and Climate Student Research

Microsatellites, also known as simple sequence repeats (SSRs), are polymorphic loci that play an important role in genome research, animal breeding, and disease control. Ranch animals are important components of agricultural landscape. The ranch animal SSR database, ranchSATdb, is a web resource which contains 15,520,263 putative SSR markers. This database provides a comprehensive tool for performing end-to-end marker selection, from SSRs prediction to generating marker primers and their cross-species feasibility, visualization of the resulting markers, and finding similarities between the genomic repeat sequences all in one place without the need to switch between other resources. The user-friendly online interface …

Evaluation Of Bio-Friendly Formulations From Siderophore-Producing Fluorescent Pseudomonas As Biocontrol Agents For The Management Of Soil-Borne Fungi, Fusarium Oxysporum And Rhizoctonia Solani, Gaber Attia Abo-Zaid, Ahmed Salah Abdullah, Nadia Abdel-Mohsen Soliman, Ebaa Ebrahim El-Sharouny, Abdulaziz A. Al-Askar, Yiming Su, Ahmed Abdelkhalek, Soraya Abdel-Fattah Sabry

Civil and Environmental Engineering Faculty Publications

Secretion of siderophores by Pseudomonas aeruginosa F2 and P. fluorescens JY3 was evaluated on chrome azurol S (CAS) agar plates and their inhibitory effect was inspected against Fusarium oxysporum and Rhizoctonia solani. Production of siderophores as biocontrol agents from F2 and JY3 was accomplished in two optimized media. Afterward, cell-free supernatants of the bacterial cultures containing siderophores were used for the preparation of two bio-friendly formulations for the management of F. oxysporum and R. solani under greenhouse conditions. The investigated bacterial isolates, F2 and JY3, showed antagonistic activity in vitro against F. oxysporum and R. solani and produced siderophores …

Boxelder Leafroller, Marion Murray, Ryan Davis

All Current Publications

The boxelder leafroller, Archips negundana, is a pest of boxelder and other trees and shrubs in parts of the United States and Canada and is commonly found in Utah. Boxelder is the primary host, but damage can also occur on raspberry, birch, elderberry, white elm, and various shrubs.This fact sheet provides information on the boxelder leafroller's life cycle, symptoms of damage, and control methods.

Parents’ Perceptions Of The Philly Goat Project’S All Abilities Ramble: A Qualitative Study Of Animal-Assisted Intervention For Intellectual And Developmental Disorders, Patricia Flaherty-Fischette, Jenée Lee, Yvonne D'Uva-Howard, Elizabeth P. Cramer, Karen Krivit, Sarah Meehan

Developmental Disabilities Network Journal

Individuals with intellectual and developmental disabilities (IDD) are a growing population. Considering the wide diversity in IDD and the financial burden of traditional treatment modalities, Animal-Assisted Interventions (AAI) has emerged as an innovative and non-traditional treatment for individuals with a range of disabilities, including individuals with IDD. To the authors’ knowledge, the present study was one of the first to explore a goat-assisted therapy experience for children with IDD. This study explored the experiences of 23 children with the All Abilities RAMble – a goat-assisted therapeutic activity offered by the Philly Goat Project (PGP). Key themes in our study included …

Ticks And Tick-Borne Diseases Of Utah, Kate V. Richardson, Ryan Davis, Ricardo Ramirez

All Current Publications

This fact sheet describes ticks and tick-borne diseases in Utah. It includes a description of ticks, their life cycle, habitat, feeding, and reproduction. The fact sheet also provides information about tick bites and safety, common tick-transmitted diseases in Utah, and the status of Lyme disease in Utah.

Controlling Soil Erosion After Wildfire And Guiding Recovery In Southern Utah, Kristina Young, Kara Dohrenwend, Matt Mcettrick, Henry Grover

All Current Publications

Wildfire is a natural part of many ecosystems in the Four Corners region (Southern Utah, Northern Arizona, Southwest Colorado, and Northwest New Mexico). However, after decades of fire suppression, the intensity and size of wildfires is increasing. This fact sheet is intended to help those in dry, monsoonal regions understand what happens to land after a wildfire and explains the processes of soil erosion. It outlines factors that influence how wildfire can affect soils and plants and provides suggestions for reducing soil erosion when necessary. The information presented is relevant to both private landowners and public lands managers whose landscapes …

Comparative Genome-Wide Analysis Of Micrornas And Their Target Genes In Roots Of Contrasting Indica Rice Cultivars Under Reproductive-Stage Drought, Simardeep Kaur, Karishma Seem, Suresh Kumar, Rakesh Kaundal, Trilochan Mohapatra

Plants, Soils and Climate Student Research

Recurrent occurrence of drought stress in varying intensity has become a common phenomenon in the present era of global climate change, which not only causes severe yield losses but also challenges the cultivation of rice. This raises serious concerns for sustainable food production and global food security. The root of a plant is primarily responsible to perceive drought stress and acquire sufficient water for the survival/optimal growth of the plant under extreme climatic conditions. Earlier studies reported the involvement/important roles of microRNAs (miRNAs) in plants’ responses to environmental/abiotic stresses. A number (738) of miRNAs is known to be expressed in …

Assessing The Needs Of Utah Dairy Farmers To Inform Extension Programming, Bruce F. Richards, Lendel Narine, Justin Clawson, Kalen Taylor, Jacob Hadfield, Jody Gale

Outcomes and Impact Quarterly

A needs assessment was conducted by the Utah State University Dairy Extension Team to identify the most urgent issues faced by Utah dairy farmers. The assessment found that the top five priority needs were implementing new technology, improving public perception of water use, managing risk, succession planning, and reducing feed costs. The results will guide dairy Extension programming in Utah. Several workshops and events have already been planned to address these needs. Overall, the assessment will help USU Extension provide research-based educational programs to meet the needs of the dairy industry in Utah.

Floating And Submerged Plants Of Utah: Pocket Field Guide, Kate Sinnott, Karin M. Kettenring

All Current Publications

This is a field guide to identifying floating and submerged plants of Utah. We include a page for each species and also a dichotomous key at the end for the pondweeds. Submerged and floating plant species play critical roles in aquatic ecosystems. They provide habitat to aquatic organisms, improve water clarity by trapping sediment, and absorb excess nutrients from the water column, among other vital services. However, they have been threatened and degraded by pollution, land conversion, and introductions of harmful species. Identifying native and non-native plants is an important component of tackling this degradation and promoting the conservation and …

Rhaphiolepis Indica Fruit Extracts For Control Fusarium Solani And Rhizoctonia Solani, The Causal Agents Of Bean Root Rot, Ahmed A. Heflish, Said I. Behiry, Abdulaziz A. Al-Askar, Yiming Su, Ahmed Abdelkhalek, Mohamed K. Gaber

Civil and Environmental Engineering Faculty Publications

Numerous strategies have been suggested to reduce dependence on synthetic products, such as physical, microbial, and natural methods. Among the natural remedies, plant extracts have emerged as a popular option owing to their eco-friendly character, ease of degradation, and harmless nature to humans. In our study, we used the acetone and hexane extracts of Rhaphiolepis indica fruit to combat two fungal pathogens that were isolated from infected bean plants and showed root rot symptoms. The two pathogens were confirmed to be pathogenic by pathogenicity assays conducted in vivo. The morphological and molecular identification by ITS-region sequencing revealed that the two …

Balsam Woolly Adelgid, Liz Rideout, Kate V. Richardson, Diane G. Alston, Ryan Davis, Darren Mcavoy, Lori R. Spears, Danielle Malesky, Liz Herbertson, Colleen Keyes

All Current Publications

This fact sheet describes balsam woolly adelgid, a tiny sucking insect that is a serious pest of true firs in forests, landscapes, and in seed and Christmas tree production. It includes a description of tree injury and symptoms, life history and identification, monitoring, management, and implications to forest health.

Speeding Snowmelt To Control Snow Mold, Justin Wyatt Clawson, Michael Pace, Eric Galloway, Matt Yost, Earl Creech

All Current Publications

The high level of snowpack in the winter of 2022–2023 created a renewed interest in speed-melting snow to prevent snow mold on winter wheat crops, golf courses, and homeowners’ lawns. Snow molds are diseases caused by fungi growing under snow cover or in cool, wet weather. Winter wheat can be infected by three types of snow molds, while turf grasses can be infected by two kinds of snow molds. Plants will have a bleached color for pink snow mold or a thin layer of white or gray mycelium leaving the plants gray or white after they dry out for gray …

Equine Navicular Syndrome, Kylie Stephens, Karl Hoopes

All Current Publications

Equine navicular syndrome has a large effect on the horse industry by accounting for up to one third of all chronic forelimb lamenesses. While the exact cause and development of this disease is still widely controversial, there are noticeable and significant changes to the internal anatomy and structures that could contribute to the possible onset of lameness. Three main causes are proposed that could play a role in the progression of this syndrome: biomechanical forces, vascular alterations, and chronic inflammation. The clinical signs of navicular syndrome can range from short choppy strides to intermittent lameness, as well as gait changes …

Utah Florist Preferences For Local Cut Flowers, Kynda R. Curtis, Melanie Stock

All Current Publications

This fact sheet provides crucial information on wholesale florist needs and preferences for local cut flowers. The information here will enhance the ability of current and potential cut flower growers to properly assess the profit potential of their decisions and assist with the long-term sustainability of their farming operations.

Understanding Irrigation Water Optimization, Burdette Barker, Matt Yost, Jody Gale, Mark Nelson

All Current Publications

Irrigation is applied to much of the cropped area of Utah to support crop production. Limited water resources and competing demand for those resources make irrigation water conservation, irrigation water optimization, and efficient use of irrigation water important components of overall water resource management. It is well known in Utah that optimal irrigation use is even more critical during drought conditions. However, optimization practices change the quantity, quality, and timing of water flows. It is important to consider the possible hydrologic impact of irrigation practice changes and the desired outcome of an optimization practice to avoid implementing a practice that …

Producing Maple Syrup From Boxelder And Norway Maple Trees, Jesse Mathews, Youping Sun, Kelly Kopp, Darren Mcavoy, Steven Price, Paul Harris, Michael Farrell, Michelle Sagers, Patrick Kelly

All Current Publications

In Utah, two common tappable maple species are boxelder, sometimes called ashleaf maple (Acer negundo), and Norway maple (Acer platanoides). Both species are abundant throughout the state, with the former primarily found in natural mid-elevation canyons and the latter extensively planted in urban landscapes. Maple syrup is a natural source of sugar and can be used in many recipes to make yummy treats and foods. The best part is that it is relatively easy to obtain and simple to make. This fact sheet reviews the syrup-making process.

Raising A Bottle-Fed Calf On A Cow/Calf Operation, Reganne K. Briggs, Katelyn Barsnick, Cheyenne Reid, Ashley Longmore

All Current Publications

Having bottle-fed calves on the ranch is common for beef producers. The most likely scenarios are a cow has died, a cow isn’t giving enough milk, or a twin has been rejected. All these scenarios are likely to happen, which results in an orphan calf. Bottle-feeding a calf is straightforward; however, there are limited resources, specifically for beef producers, looking for bottle-fed calf management strategies. When bottle-feeding a beef calf, there are some things to consider, such as feeding colostrum, selecting and using milk replacers, and weaning the calf. Many of the resources on bottle-calf management come from the dairy …

Mice As An Animal Model For Japanese Encephalitis Virus Research: Mouse Susceptibility, Infection Route, And Viral Pathogenesis, Jordan C. Frank, Byung-Hak Song, Young-Min Lee

Animal, Dairy, and Veterinary Science Faculty Publications

Japanese encephalitis virus (JEV), a zoonotic flavivirus, is principally transmitted by hematophagous mosquitoes, continually between susceptible animals and incidentally from those animals to humans. For almost a century since its discovery, JEV was geographically confined to the Asia-Pacific region with recurrent sizable outbreaks involving wildlife, livestock, and people. However, over the past decade, it has been detected for the first time in Europe (Italy) and Africa (Angola) but has yet to cause any recognizable outbreaks in humans. JEV infection leads to a broad spectrum of clinical outcomes, ranging from asymptomatic conditions to self-limiting febrile illnesses to life-threatening neurological complications, particularly …