Life Sciences Commons^™

Valorization Of Seafood Waste For Food Packaging Development, Zhijing Zhan, Yiming Feng, Jikai Zhao, Mingyu Qiao, Qing Jin

School of Earth, Environmental, & Marine Sciences Faculty Publications

Packaging plays a crucial role in protecting food by providing excellent mechanical properties as well as effectively blocking water vapor, oxygen, oil, and other contaminants. The low degradation of widely used petroleum-based plastics leads to environmental pollution and poses health risks. This has drawn interest in renewable biopolymers as sustainable alternatives. The seafood industry generates significant waste that is rich in bioactive substances like chitin, chitosan, gelatins, and alginate, which can replace synthetic polymers in food packaging. Although biopolymers offer biodegradability, biocompatibility, and non-toxicity, their films often lack mechanical and barrier properties compared with synthetic polymer films. This comprehensive review …

The Fishing Cat: New Insights On Distribution In The Northern Sector Of Chitwan National Park, Nepal, Rupesh Maharjan, Rama Mishra, Jhamak Bahadur Karki

School of Earth, Environmental, & Marine Sciences Faculty Publications

Fishing cat Prionailurus viverrinus (Bennett, 1833) is an elusive and medium-sized feline species with distinguished marking patterns on their head and body separating them from other feline species. Despite being distributed widely among diverse geographic locations, their abundance has declined due to significant habitat loss and human disturbances. There exists a substantial research gap regarding their abundance and distribution in Nepal. In this article, we presented the updated documentation of the fishing cat distribution in the northern sector of Chitwan National Park through our species-focused camera trapping survey. The study was carried out between April and May 2021 with a …

Lactobacillus Rhamnosus Interactions With Foodborne Pathogens And Impact On Plant Productivity In A Model Hydroponic System, Esther Titilope Oginni

Theses and Dissertations

Food production is a global problem, and this has been further compounded with changes in weather patterns necessitating the need for a sustainable approach to production. Chapter 1 introduces the broad concept of the Control environment agriculture (CEA) and then narrows it doe to the hydroponic system its food safety risks. The CEA has been employed alongside conventional practices methods to improve meet the demand for food without placing pressure on the land available. Foodborne outbreaks have been recorded in the production medium and are therefore susceptible to contamination. In Chapter 2, the study focused on using Lactobacillus rhamnosus and …

Asynchronous Movement Patterns Between Breeding And Stopover Locations In A Long-Distance Migratory Songbird, Theodore J. Zenzal Jr., Andrea Contina, Hannah B. Vander Zanden, Leanne K. Kuwahara, Daniel C. Allen, Kristen M. Covino

School of Integrative Biological & Chemical Sciences Faculty Publications

The species-specific migratory patterns and strategies of many songbirds remain unknown or understudied, as research in animal ecology is biased toward the breeding period, with the fewest studies on the migratory period across taxa. Identifying large-scale spatiotemporal migratory patterns is challenging, as individuals within a species may vary in their migratory behavior and strategies. The Yellow Warbler (Setophaga petechia) is a Nearctic-Neotropical migrant that is relatively well studied during the breeding season, but its species-wide migratory patterns remain understudied. Our aim in studying Yellow Warbler movement ecology was to characterize temporal migration patterns during fall migration. We sought …

Development And Characterization Of Β-Cyclodextrin Inclusion Complexes With Enzalutamide For Easing Administration And Efficacy Of The Drug In Crpc, Meghana Kolli

Theses and Dissertations

Prostate cancer (PC) is prevalent among men in the US, often leading to castration-resistant prostate cancer (CRPC) with therapy. This is marked by androgen receptor (AR) overexpression, resistance, mutations, and splice variants. Enzalutamide (ENZ), a nonsteroidal antiandrogen, surpasses first-gen AR inhibitors like Bicalutamide. It's the go-to drug for CRPC. We explore ENZ for self-assembly complex formation, crucial for CRPC treatment. Using the solvent evaporation method, we developed β-cyclodextrin and ENZ self-assembly complexes (β-CD-ENZ). β-CD acts as both agent and solubilizer, FDA-approved and safe. Efficacy was tested on PC cell lines (C4-2B) and (22Rv1), including 3D cultures for cytotoxicity and a …

Association Of Longitudinal Changes In 24-H Blood Pressure Level And Variability With Cognitive Decline, Jesus D. Melgarejo, Kristina Vatcheva, Silvia Mejia-Arango, Sokratis Charisis, Luis J. Mena, Rosa P. Mavarez, Antonio Garcia, Ney Alliey Rodriguez, John Blangero, Gladys Maestre

School of Medicine Publications

Objective:

A high office blood pressure (BP) is associated with cognitive decline. However, evidence of 24-h ambulatory BP monitoring is limited, and no studies have investigated whether longitudinal changes in 24-h BP are associated with cognitive decline. We aimed to test whether higher longitudinal changes in 24-h ambulatory BP measurements are associated with cognitive decline.

Methods:

We included 437 dementia-free participants from the Maracaibo Aging Study with prospective data on 24-h ambulatory BP monitoring and cognitive function, which was assessed using the selective reminding test (SRT) and the Mini-Mental State Examination (MMSE). Using multivariate linear mixed regression models, we analyzed …

Invertebrate Diversity From Deep Wells Of The Southern Boundary Of The Edwards Aquifer Of Southcentral Texas, Kathryn E. Perez, Valentin Cantu, Vanessa Saenz, J. Randy Gibson, Patricia Duncan, Benjamin T. Hutchins

School of Integrative Biological & Chemical Sciences Faculty Publications

This study examined the invertebrate fauna from three deep wells located at the Uvalde National Fish Hatchery in south-central Texas. These wells provide a water source from geological strata of the southern boundary of the Edwards Aquifer ranging in age from Lower Cretaceous to Recent. Forty-seven collections, sampled on a continuous basis from November 2020 to January 2022, produced a total of 738 specimens comprising 15 different species. These collections reveal a diverse crustacean assemblage along with dominant numbers of the stygobiotic snail Balconorbis uvaldensis. This study provides a preliminary survey for future investigations of unidentified crustacean species as …

A Pilot Study On Particulate Matter Concentrations From Cooking And Its Effects On Indoor Air Pollution In A Mexican American Household In Mission, South Texas, Usa, Sai Deepak Pinakana, Carlos Garcia Patlan, Esmeralda Mendez, Amit U. Raysoni

School of Earth, Environmental, & Marine Sciences Faculty Publications

This pilot study focuses on particulate matter (PM) while cooking in a South Texan household. Dishes such as Beef, Burger, Fish, Chicken, Egg Sandwich, and Hotdog were prepared. Indoor PM levels were compared with outdoor PM levels. A DustTrak DRX was used to monitor the PM released during the cooking process. PM2.5 levels were highest while cooking beef, 162.79 + 209.62 μg m−3. Hot Dog preparation resulted in the lowest PM2.5 concentration of 27.72 + 5.58 μg m−3. Indoor PM2.5 levels were observed to be greater in contrast to outdoor levels when compared to the outdoor levels (96 words).

Neutrophil To Lymphocyte Ratio (Nlr) In Captive Olive Baboons (Papio Anubis): The Effects Of Age, Sex, Rearing, Stress, And Pregnancy, Sarah J. Neal, Angela M. Achorn, Steven J. Schapiro, William D. Hopkins, Joe H. Simmons

Anthropology Faculty Publications

In apes and humans, neutrophil to lymphocyte ratio (NLR) can be used as a predictive indicator of a variety of clinical conditions, longevity, and physiological stress. In chimpanzees specifically, NLR systematically varies with age, rearing, sex, and premature death, indicating that NLR may be a useful diagnostic tool in assessing primate health. To date, just one very recent study has investigated NLR in old world monkeys and found lower NLR in males and nursery-reared individuals, as well as a negative relationship between NLR and disease outcomes. Given that baboons are increasingly used as research models, we aimed to characterize NLR …

Using Bayesian Multispecies Models To Evaluate Fish And Invertebrate Detection Probability And Distribution In The Hypersaline Bahia Grande Tidal Basin, Roy M. Ulibarri, Catherine M. Eckert, David Hicks, David Montagne, Brandon Jones, David R. Stewart

School of Earth, Environmental, & Marine Sciences Faculty Publications

Objective

In 2000, the Laguna Atascosa National Wildlife Refuge acquired the Bahia Grande (Texas) management unit, a space that had lain barren and arid for 70 years. A large cooperative partnership launched a restoration project to replenish the basin and recover its original tidal hydrology. In 2005, the construction of a pilot channel successfully restored water throughout the basin, and plans to eventually widen the channel were developed. Our study aims to evaluate an estuarine habitat restoration by assessing ecological drivers and the impacts on species diversity.

Methods

We evaluated species richness, detection/occupancy rates, and species–habitat relationships, and we estimated …

Stationary Bike Injuries Prompting Emergency Department Presentation In Pediatric Patients: An Epidemiological Study, Kathleen L. Harwood, David Kell, Kevin J. Orellana, Nathan D. Markiewitz, Brendan A. Williams

School of Medicine Publications

Objectives: Potential harms of stationary bike injuries in pediatric patients have been highlighted in the literature, but prior work is limited to case series and without population-level analysis. This study's purpose is to examine the epidemiology of pediatric stationary bike injuries occurring in the US over the last decade using a national database.

Methods: Injuries resulting from stationary bike use in pediatric patients from 2012-2021 were identified using coding from the National Electronic Injury Surveillance System (NEISS) database. Patient demographics, injury characteristics, and case narratives were examined. National annual estimates of injury frequency/incidence were calculated using weighting and survey package …

The Biology Of The Facultative Halophyte Atriplex Patula L., Richard Stalter, Robert I. Lonard

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Atriplex patula L. is a facultative halophyte that tolerates low to moderate levels of salinity and occurs on the margins of coastal salt marshes, roadsides, in agricultural fields, and in other areas where disturbance has occurred. The genus Atriplex L. is represented by about 250 species globally and has recently been included in the family Amaranthaceae subfamily Chenopodioidae. Atriplex patula was possibly introduced in North America from Europe, Asia or North Africa in the 18th century. Because the stems and leaves accumulate and store heavy metal pollutants, A. patula has been suggested for use in habitat remediation.

Biotechnology For Sustainable Materials: Innovating Today For A Greener Tomorrow, Shashi Kant Bhatia, Saurabh Bhatia, Maria Eugenia Inda-Webb, Konstantina Kourmentza, Tae Seok Moon, Vijai Singh, Vishal Ahuja, Jingbo Li, Sanjeet Mehariya, Jikai Zhao

School of Earth, Environmental, & Marine Sciences Faculty Publications

No abstract provided.

Effects Of Fast And Slow-Wilting Soybean Genotypes On Fall Armyworm (Spodoptera Frugiperda) Growth And Development, Jessica Ayala, Alejandro Vasquez, Devi Balakrishnan, Evelyn Madrigal, Justin George, Rupesh R. Kariyat

School of Integrative Biological & Chemical Sciences Faculty Publications

Soybean (Glycine max) is the most important plant protein source, and Fall Armyworm (FAW, Spodoptera frugiperda) is considered a major pest. This study aimed to examine the impact of FAW feeding on soybean accessions that vary in their water use efficiency (WUE) traits, by examining FAW growth and life history parameters along with plant growth response to pest damage. Soybean accessions were grown in a greenhouse and exposed to FAW larval feeding for 48 h at three different soybean growth stages: V3, R3, and R6. The growth and development of the FAW and soybeans were monitored. Results showed …

Eviction-Driven Infanticide And Sexually Selected Adoption And Infanticide In A Neotropical Parrot, Steven R. Beissinger, Karl S. Berg

School of Integrative Biological & Chemical Sciences Faculty Publications

Significance

Infanticide and adoption are puzzling forms of sexual conflict and cooperation, respectively. However, both may be explained by sexual selection, where an individual later reproduces with the parent whose offspring it killed or adopted. While sexually selected infanticide is well known, evidence for sexually selected adoption is anecdotal. Our long-term study of a parrotlet in Venezuela found infanticide attacks were mostly enacted by nonbreeding pairs attempting to evict parents from their nests to usurp the cavity. Infanticide attacks occurred less often at nests where a parent died, and adoption by stepparents was as common as infanticide. Becoming an adoptive …

Effect Of Short-Term Exposure To Pesticide Mixture On Tissue Architecture, Body Fluid Conditions, Nitrative Stress And Osmoregulation Of American Oyster, Crassostrea Virginica, Asif Ahmed

Theses and Dissertations

Pesticides are used widely to control weeds and pests in agricultural settings but harm non-target aquatic organisms. We examined the effect of short-term exposure (one week) to pesticide mixtures (low dose: 0.5 μg/L Roundup®, 0.5 μg/L 2,4-D, and 0.4 μg/L atrazine; high dose: 1 μg/L Roundup®, 1 μg/L 2,4-D, and 0.8 μg/L atrazine) on American oyster (Crassostrea virginica, an important shellfish and seafood species) under controlled laboratory conditions. Histological analyses demonstrated the atrophy in oysters' gills, digestive glands, and connective tissue. The protein concentrations and glucose levels dropped significantly in the high-dose treatment. The number of hemocytes in connective tissue …

Investigating The Demise Of East Bank Ridge Through Local Fishery Perceptions And Fish Population Metrics, Marissa R. Lamb

Theses and Dissertations

East Bank Ridge is a natural reef formation located 18 nautical miles off the coast of South Padre Island, covering an area of over 80 km2, with the southern portion extending into Mexico. There is no previous record of the structural composition or fish community present at the site. The ridge was a well-known fishing site in the local community over the past decades but catch rates have decreased substantially according to personal accounts from local anglers. In this study, areas of reef habitat were identified, and fish presence and abundance metrics were determined at East Bank Ridge. These measures …

Genetics Of Lrg1 And Its Association With The Cardiometabolic Risk Traits In Mexican American Children And Adolescents, Aaron Rene Reyna

Theses and Dissertations

Leucine-rich alpha-2-glycoprotein 1 (LRG1) has become a major topic of research due to an increasing number of studies demonstrating its unique role in the pathogenesis of multiple human diseases including diabetes and cardiovascular disease. LRG1 has recently been identified as an adipokine, a signaling molecule secreted by adipose tissue. Furthermore, it has been correlated with intercellular signaling that promotes diet-induced hepatosteatosis and insulin resistance in mice. In this study, LRG1 serum concentrations were measured in a family-based cohort of 571 non-diabetic Mexican American children and adolescents aged 6-17 years old (mean age = 11.76 years and females = 48.2%) to …

Foxo4 Impacts Akt In Glioblastoma Multiforme, David Uribe

Theses and Dissertations

Glioblastoma Multiforme (GBM) is an aggressive cancer with patients surviving 13 months on average after diagnosis. The PI3K-PKB/AKT pathway is conserved from flies to mammals. The PI3K signaling pathway aids in the growth and survival of tumor cells. Forkhead box transcription factors subclass O (FOXO1, FOXO3, and FOXO4) act as tumor suppressors in some contexts. In GBM, our group and others showed that FOXO transcription factors drive stem gene expression to promote growth and survival. We disrupted the FOXO4 gene in U87MG cells using CRISPR Cas9 genome editing and performed RNA-seq to identify targets of this …

Development And Application Of Environmental Dna (Edna) Techniques To Detect Rough-Footed And Yellow Mud Turtles: A Seasonal Field Study To Detect Turtle Edna, Sakib Tahmid Rishan

Theses and Dissertations

The application of environmental DNA (eDNA) has improved the ability of humans to detect the existence and geographical distribution of aquatic organisms. The present study highlights the development and application of eDNA techniques to detect rough-footed mud turtles and yellow mud turtles in different areas in Texas. Both mud turtle species were successfully detected in lakes/resacas using direct eDNA sampling techniques. Moreover, passive eDNA sampling techniques were conducted using different membrane filters to detect mud turtle species. The findings indicated that only the mixed cellulose esters (MCE) filter membranes effectively identified eDNA of yellow mud turtles in the fall, but …

Effects Of Nitrogen Or Phosphorus Depletion On Lipid Production In Bracteacoccus Sp. And Tetrastrum Sp., Cassandra J. Po

Theses and Dissertations

As global energy demands have risen in the past decades, various species of microalgae have been chosen and used as sources of lipids for third-generation biofuel. I selected two species of green microalgae, Bracteacoccus sp. and Tetrastrum sp., from isolates from South Texas to determine their potential as sources of biofuel. For comparison, Chlamydomonas reinhardtii was used as a model organism. To evaluate lipid productivity under different nutrient regimens, the microalgae were grown in nutrient-replete growth media until the population had reached the end of the logarithmic phase (roughly 14 days), and then transferred into growth media depleted of nitrogen …

Role Of Interferon-Induced Protein With Tetratricopeptide Repeats 3 (Ifit3) In Hiv-1 Associated Neurodegenerative Disorder And Alzheimer’S Disease, Ranjit Kumar Das

Theses and Dissertations

The advent of cART has revolutionized the management of HIV-1 infection and saved the lives of millions of people worldwide. Yet, HAND continues to be clinically relevant with aging people with HIV-1 (PWH). The underlying mechanism of HAND remains poorly understood. In this regard. Signal transducer and activator of transcription 1(STAT1) and IFIT3 genes have been shown to induce neuroinflammation and neuronal cell death. However, the role of IFIT3 in HAND pathology has not been well established. Therefore, the present work investigated the significant role of IFIT3 gene in development of HAND. In the current study, …

Year-Round Co2 Emissions From The Drawdown Area Of A Tropical Reservoir: Strong Seasonal And Spatial Variation, Ícaro Barbosa, José R. Paranaíba, Sebastian Sobek, Sarian Kosten, Rafael M. Almeida, Vitor Duque, Natália Mendonça, Nathan Barros, Raquel Mendonça

School of Earth, Environmental, & Marine Sciences Faculty Publications

Highlights

• Drawdown areas emitted 80% of reservoir CO2 with just 1/5 area.

• Emissions from reservoir shorelines near forests were greater than from areas near grassland.

• CO2 emissions increase with the distance from the water.

• Estimates of reservoir drawdown CO2 flux vary by ∼ 300 % across the seasons examined here.

• There was no discernible difference in CO2 emissions between day and night-time.

• A significant increase in CO2 fluxes was observed 30 min after a rewetting event.

Abstract

A growing body of literature points to drawdown areas as important sources of atmospheric CO2 within reservoirs. Yet seasonal and temporal patterns of …

Disaster Plant Pathology: Smart Solutions For Threats To Global Plant Health From Natural And Human-Driven Disasters, Berea A. Etherton, Robin A. Choudhury, Ricardo I. Alcalá Briseño, Romaric A. Mouafo-Tchinda, Aaron I. Plex Sulá, Manoj Choudhury, Ashish Adhikari, Si Lin Lei, Nattapol Kraisitudomsook, Jacobo Robledo Buritica

School of Earth, Environmental, & Marine Sciences Faculty Publications

Disaster plant pathology addresses how natural and human-driven disasters impact plant diseases, and the requirements for smart management solutions. Local to global drivers of plant disease change in response to disasters, often creating environments more conducive to plant disease. Most disasters have indirect effects on plant health through factors such as disrupted supply chains and damaged infrastructure. There is also the potential for direct effects from disasters, such as pathogen or vector dispersal due to floods, hurricanes, and human migration driven by war. Pulse stressors such as hurricanes and war require rapid responses, while press stressors such as climate change …

Skimming Genomes For Systematics And Dna Barcodes Of Corals, Andrea M. Quattrini, Luke J. Mccartin, Erin E. Easton, Jeremy Horowitz, Herman H. Wirshing, Hailey Bowers, Kenneth Mitchell, María Del P. González-García, Makiri Sei, Catherine S. Mcfadden

School of Earth, Environmental, & Marine Sciences Faculty Publications

Numerous genomic methods developed over the past two decades have enabled the discovery and extraction of orthologous loci to help resolve phylogenetic relationships across various taxa and scales. Genome skimming (or low-coverage genome sequencing) is a promising method to not only extract high-copy loci but also 100s to 1000s of phylogenetically informative nuclear loci (e.g., ultraconserved elements [UCEs] and exons) from contemporary and museum samples. The subphylum Anthozoa, including important ecosystem engineers (e.g., stony corals, black corals, anemones, and octocorals) in the marine environment, is in critical need of phylogenetic resolution and thus might benefit from a genome-skimming approach. We …

Utilizing Nanodiamond For Antiretroviral Drug Delivery To Hiv Infected Microglial Cells, Ebenezer Owusu

Theses and Dissertations

Combination antiretroviral therapy (cART) is widely used to treat HIV infection, but it fails to eradicate viral reservoirs, such as microglia in the brain, leading to HIV-associated neurological disorders (HAND). Advances in nanomedicine have enabled the development of targeted drug delivery systems, and nanodiamonds (NDs) and fluorescent nanodiamonds (FNDs) have emerged as a potential solution due to their biocompatibility, non-toxic nature, and efficiency as drug carriers. Our study builds on previous findings that NDs can cross the blood-brain barrier (BBB), aiming to utilize NDs and FNDs for their added traceability. We propose a multifunctional nanodrug platform that leverages NDs and …

Continuous Mowing Differentially Affects Floral Defenses In The Noxious And Invasive Weed Solanum Elaeagnifolium In Its Native Range, Alejandro Vasquez, Alexa Alaniz, Robert K. Dearth, Rupesh R. Kariyat

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

In weeds, disturbance has been found to affect life history traits and mediate trophic interactions. In urban landscapes, mowing is an important disturbance, and we previously showed that continuous mowing leads to enhanced fitness and defense traits in Solanum elaeagnifolium, Silverleaf Nightshade (SLN). However, most studies have been focused on foliar defenses, ignoring floral defenses. In this study we examined whether continuous mowing affected floral defenses in SLN using mowed and unmowed populations in South Texas, their native range. We found flowers of mowed SLN plants larger but lighter than unmowed plants. Additionally, flowers on plants that were mowed …

Toward A Coordinated Understanding Of Hydro-Biogeochemical Root Functions In Tropical Forests For Application In Vegetation Models, Daniela F. Cusack, Bradley O. Christoffersen, Chris M. Smith-Martin, Kelly M. Andersen, Amanda L. Cordeiro, Katrin Fleischer, S. Joseph Wright, Nathaly R. Guerrero-Ramírez, Laynara F. Lugli, Lindsay A. Mcculloch

School of Integrative Biological & Chemical Sciences Faculty Publications

Tropical forest root characteristics and resource acquisition strategies are underrepresented in vegetation and global models, hampering the prediction of forest–climate feedbacks for these carbon-rich ecosystems. Lowland tropical forests often have globally unique combinations of high taxonomic and functional biodiversity, rainfall seasonality, and strongly weathered infertile soils, giving rise to distinct patterns in root traits and functions compared with higher latitude ecosystems. We provide a roadmap for integrating recent advances in our understanding of tropical forest belowground function into vegetation models, focusing on water and nutrient acquisition. We offer comparisons of recent advances in empirical and model understanding of root characteristics …

The Effects Of Genetic And Modifiable Risk Factors On Brain Regions Vulnerable To Ageing And Disease, Jordi Manuello, Joosung Min, Paul Mccarthy, Fidel Alfaro-Almagro, Soojin Lee, Stephen Smith, Lloyd T. Elliott, Anderson M. Winkler, Gwenaëlle Douaud

School of Medicine Publications

We have previously identified a network of higher-order brain regions particularly vulnerable to the ageing process, schizophrenia and Alzheimer’s disease. However, it remains unknown what the genetic influences on this fragile brain network are, and whether it can be altered by the most common modifiable risk factors for dementia. Here, in ~40,000 UK Biobank participants, we first show significant genome-wide associations between this brain network and seven genetic clusters implicated in cardiovascular deaths, schizophrenia, Alzheimer’s and Parkinson’s disease, and with the two antigens of the XG blood group located in the pseudoautosomal region of the sex chromosomes. We further reveal …

Β-Cyclodextrin-Enzalutamide Self-Assembly Complexes For Prostate Cancer Therapy, Meghana Kolli, Rahul Tiwari, Subhash C. Chauhan, Yonghong Zhang, Neeraj Chauhan, Alba C. M, Murali Yallapu

Research Symposium

Background:Castrate circumstances brought on by therapy give rise to castrationresistant prostate cancer (CRPC), which thereafter develops androgen receptor (AR) resistance. AR inhibitors are frequently used to obstruct AR translocation in cells that overexpress AR and slow the progression of cancer by reducing the number of AR receptors that are accessible. In comparison to Bicalutamide, a first-generation AR inhibitor, the hydrophobic drug Enzalutamide has demonstrated higher effectiveness. We investigate Enzalutamide as a viable option for the formation of self-assembly complexes because AR downregulation is still the major treatment for CRPC.

Methods:Enz-loaded self-assembly complexes (enz-β-CD) formulation was developed using solvent …