Life Sciences Commons^™

Assessing Infection Patterns In Chinese Mystery Snails From Wisconsin, Usa Using Field And Laboratory Approaches, Brittany Harried, Kyle Fischer, Kathryn E. Perez, Gregory J. Sandland

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

The success of invasive species establishment in new habitats depends, in part, on interactions with other members of the invaded community. Cipangopaludina chinensis, the Chinese mystery snail (CMS), is an exotic, invasive snail in North America. Since its introduction in the 1890s, CMS have spread to a number of watersheds across the United States and parts of Canada. This invasion has generated questions about the snail’s capacity to serve as a host for parasites within local habitats, including parasites with conservation implications. To begin addressing these questions, we necropsied 147 wild-caught snails from 22 lakes across Wisconsin, finding only two …

Juniperus Communis In Azerbaijan: Analyses Of Nrdna And Cpdna Regions, Robert P. Adams, Vahid Farzaliyev, Alexander N. Tashev, Andrea E. Schwarzbach

Health & Biomedical Sciences Faculty Publications

Juniperus 'pygmaea' from Azerbaijan was analyzed by DNA sequence data from nrDNA plus four cp DNA regions (4315 bp) and found in a clade with J. communis 'oblonga' (= J. communis) Armenia, not with J. c. forma pygmaea of Bulgaria. It seems prudent to not recognize this variant taxonomically but treat it as J. communis.

Octocoral Mitochondrial Genomes Provide Insights Into The Phylogenetic History Of Gene Order Rearrangements, Order Reversals, And Cnidarian Phylogenetics, Diego F. Figueroa, Amy R. Baco

School of Earth, Environmental, & Marine Sciences Faculty Publications

We use full mitochondrial genomes to test the robustness of the phylogeny of the Octocorallia, to determine the evolutionary pathway for the five known mitochondrial gene rearrangements in octocorals, and to test the suitability of using mitochondrial genomes for higher taxonomic-level phylogenetic reconstructions. Our phylogeny supports three major divisions within the Octocorallia and show that Paragorgiidae is paraphyletic, with Sibogagorgia forming a sister branch to the Coralliidae. Furthermore, Sibogagorgia cauliflora has what is presumed to be the ancestral gene order in octocorals, but the presence of a pair of inverted repeat sequences suggest that this gene order was not conserved …

Empirical Modelling Of Solid-Blocking Effect In A Blazka Respirometer For Gag, A Large Demersal Reef Fish, Richard J. Kline, Daryl C. Parkyn, Debra J. Murie

School of Earth, Environmental, & Marine Sciences Faculty Publications

The effect of solid-body blocking in a large (257 l) Blazka-style respirometer was examined in gag Mycteroperca microlepis. Gag ranging from 376-690 mm total length were tested at velocities from 10 to 91 cm s-1 . The solid blocking effect (SBE) due to fish size, swimming speed, and water temperature was investigated by direct measurement of flow velocity at the area of the fish’s maximum girth as compared to measurements at the rear (center) of the swimming chamber. Three models were compared as corrections for the SBE: the standard Bell and Terhune (BT), a novel modification of the Bell and …

The Acute Effects Of Aerobic Exercise Durations On Arterial Compliance In Recreationally Active Males, Joe Angel Lopez

Theses and Dissertations - UTB/UTPA

PURPOSE: The purposes of this study were to 1) examine the acute effects of different aerobic exercise durations on large (LAC) and small (SAC) arterial compliance, 2) examine the acute effects of different aerobic exercise durations on central pulse wave velocity (cPWV), peripheral pulse wave velocity (pPWV), Aortic Index (AIx), and 3) to examine the acute effects of aerobic exercise durations on hemodynamics.

METHODS: Eighteen male subjects (age= 23.4±2.0) performed a maximal aerobic stress test (Bruce protocol) in order to estimate VO2max. Participants were required to meet in the lab fasted for at least 8 hours for three …

The Acute Effects Of Different Warm-Up Techniques Of Power Output, Agility, And Flexibility In Athletes, Kristopher Manuel Nava

Theses and Dissertations - UTB/UTPA

Purpose: The purpose of this study is: 1) To determine which warm-up technique (general warm-up (GW), dynamic warm-up (DW), weighted vest warm-up using body weight percentage [VW], and elastic exercise band training system warm-up [EEBTSW]) will provide the best and longest effect on athletes’ performance regarding power output, agility, and flexibility. 2) To compare if there are any differences in power output, agility, and flexibility when using different resistance protocols (VW and EEBTSW) as warm-up techniques. 3) To determine which warm-up will benefit the athletes’ performance. 4) To compare the hemodynamic responses to different warm-up techniques.

Methods: Thirty-one male (age= …

An Acid-Bath Technique To Break Seed Dormancy In Common Sunflower, Helianthus L. Annuus (Asteraceae), Yuridia Patricia Gandy, Michael W. Persans, Kenneth R. Summy

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Chemistry)

The phenomenon of seed dormancy is widespread in plants and serves to prevent all or most of a given population from germinating at the “wrong” time, e.g., during an unusually mild fall in an area subject to typically harsh winters. Seed dormancy is an effective survival strategy in many plant populations, but may greatly complicate efforts to establish large cohorts of seedlings (groups of similar age or developmental stage) needed for re-search and other purposes. In an effort to break seed dormancy in common sunflower, Helianthus annuus L. (Asteraceae), we conducted experiments designed to compare germination times and overall germination …

Detection Of Temporal Changes In Vegetative Cover On South Padre Island, Texas Using Image Classifications Derived From Aerial Color-Infrared Photographs, Ruben A. Mazariegos, Kenneth R. Summy, Frank W. Judd, Robert I. Lonard, James H. Everitt

Physics & Astronomy Faculty Publications

Supervised image classifications developed from 23 x 23 cm aerial color-infrared aerial photographs (1:5,000 scale) were used to evaluate temporal changes in vegetative cover occurring within three 150 x 300-m research sites on South Padre Island, Texas. Use of high-resolution digitized imagery (ground pixel resolution of ca. 0.1 m) and survey-grade GPS for positional measurements of ground control points (20-25 1.0m2 targets within each research site) resulted in consistently high levels of geometric accuracy, with root mean square errors (RMSEs) ranging between 0.397 – 2.867. Similarly, use of relatively simple information categories (dry and wet sand, live and dead vegetative …

Predicting The Response Of The Amazon Rainforest To Persistent Drought Conditions Under Current And Future Climates: A Major Challenge For Global Land Surface Models, E. Joetzjer, C. Delire, H. Douville, P. Ciais, B. Decharme, R. Fisher, Bradley O. Christoffersen, J. C. Calvet, A. C.L. Da Costa, L. V. Ferreira

School of Integrative Biological & Chemical Sciences Faculty Publications

While a majority of global climate models project drier and longer dry seasons over the Amazon under higher CO2 levels, large uncertainties surround the response of vegetation to persistent droughts in both present-day and future climates. We propose a detailed evaluation of the ability of the ISBACC (Interaction Soil-Biosphere-Atmosphere Carbon Cycle) land surface model to capture drought effects on both water and carbon budgets, comparing fluxes and stocks at two recent throughfall exclusion (TFE) experiments performed in the Amazon. We also explore the model sensitivity to different water stress functions (WSFs) and to an idealized increase in CO2 concentration and/or …

A Preventive Maintenance Framework In Dairy Production Operations, Maria F. Vargas

Theses and Dissertations - UTB/UTPA

Dairy operations suffer frequent stops. Product shrinkage is a consequence of downtime, which includes losses of packaging material, scraped finish product and capacity. This work proposes a troubleshooting methodology to identify causes of downtime, estimation of waste cost, and minimization of operation disruptions by applying a combination of a cost function to assess waste, and performance measurements. The drinkable yogurt process is evaluated to find the principal areas for wasted bottles and yogurt. In order to make a decision about which of those sources to address, a General Cost Function is used to estimate waste cost which include measurements that …

Associations Between Pqct-Based Fat And Muscle Area And Density And Dxa-Based Total And Leg Soft Tissue Mass In Healthy Women And Men, Vanessa D. Sherk, Robert S. Thiebaud, Zhaojing Chen, Murat Karabulut, So Jung Kim, Debra A. Bemben

Health & Biomedical Sciences Faculty Publications

Peripheral Quantitative Computed Tomography (pQCT) can be used for muscle and fat area and density assessments. These may independently influence muscle and fat mass measurements from Dual Energy X-ray Absorptiometry (DXA).

Objective: To determine associations between pQCT-derived soft tissue density and area measures and DXA-derived soft tissue mass.

Methods: Linear regression models were developed based on BMI and calf fat and muscle cross-sectional area (FCSA and MCSA) and density measured by pQCT in healthy women (n=76) and men (n=82) aged 20-59 years. Independent variables for these models were leg and total bone-free lean mass (BFLM) and fat mass (FM) measured …

Relationship Between Stream Velocity & Depth And Snail Size Distribution & Density Of The Balcones Elimia, Elimia Comalensis (Pilsbry, 1890) (Gastropoda: Pleuroceridae) In Comal Springs, Texas, Kathryn E. Perez, Russell L. Minton, Scott A. Reese, David M. Haye

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Pleurocerid snails are important components of aquatic ecosystems and the majority of species are threatened or endangered. This study describes aspects of the life-history of Elimia comalensis, specifically population density in relationship to water velocity and depth, and seasonal change. Also examined are spatial segregation of different E. comalensis age groups and the relationship of snail size (proxy for age) to 110w, depth, and seasonal change. The study was carried out in the lotic portion of spring run 3, Coma! Springs, New Braunfels, Texas, by quadrat sampling at 10 in intervals from the spring head to Landa Lake in Fall. …

Preliminary Transcriptome Analysis And Homeobox Gene Isonlation For Tomocerus Minor (Entomobryomorpha: Tomoceridae), Alejandro Alaniz

Theses and Dissertations - UTB/UTPA

Collembola, or “springtails,” are small, edaphic arthropods classified as Entognatha; a group traditionally included as part of Hexapoda and sister-group to the true insects. However, some genetic studies suggest that they may instead have derived from a separate crustacean lineage rather than from a proto-Insecta lineage. The unique abdominal structures that develop on the collembolan ventral abdomen appear to be appendicular in origin – atypical to the canonical, appendage-less insect abdomen ground plan. I developed culture and embryo fixation protocols for; and sequenced and analyzed a multi-stage embryo transcriptome for Tomocerus minor (Collembola: Entomobryomorpha). This analysis annotated 14,417 distinct genes, …

Differential Response By Bronzed Cowbirds To Songs Of Potential Hosts In The Lower Rio Grande Valley Of Texas, Mary J. Janecka, Timothy Brush

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

How bronzed cowbirds (Molothrus aeneus) find nests to parasitize is poorly known, especially whether they use host vocalizations as sources of information about nest location and status. We conducted 120 point-count surveys, followed by broadcasts of host songs, at Santa Ana National Wildlife Refuge in the Lower Rio Grande Valley of Texas in 2008 and 2009. Bronzed cowbirds responded to songs of potential host species by flying toward the source of the playback as single females or males or multiple females and males. Bronzed cowbirds responded in greater numbers to the songs of oriole species (Icterus) …

Establishment And Spread Of A Single Parthenogenic Genotype Of The Mediterranean Arundo Wasp, Tetramesa Romana, In The Variable Climate Of Texas, John A. Goolsby, John F. Gaskin, Daniel V. Tarin, Alan E. Pepper, Don C. Henne, Allan Auclair, Alexis Racelis, Kenneth R. Summy, Patrick J. Moran, Donald B. Thomas

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

As part of a biological control program for the invasive weed, Arundo donax L., several genotypically unique populations of the parthenogenetic stemgalling wasp, Tetramesa romana Walker (Hymenoptera: Eurytomidae), from Spain and France were released in an infested riparian zone along the Rio Grande from Brownsville to Del Rio, TX. An adventive population of the wasp of unknown origin with limited distribution in Texas was also discovered, evaluated, and released as part of the program. More than 1.2 million wasps representing the mixture of genotypes were aerially released from 2009 to 2011. Wasps dispersed from their original release locations and now …

Pathogenic Landscape Of Transboundary Zoonotic Diseases In The Mexico–Us Border Along The Rio Grande, Maria Dolores Esteve-Gassent, Adalberto A. Pérez De León, Dora Romero-Salas, Teresa Patricia Feria-Arroyo, Ramiro Patino, John A. Goolsby

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Transboundary zoonotic diseases, several of which are vector borne, can maintain a dynamic focus and have pathogens circulating in geographic regions encircling multiple geopolitical boundaries. Global change is intensifying transboundary problems, including the spatial variation of the risk and incidence of zoonotic diseases.The complexity of these challenges can be greater in areas where rivers delineate international boundaries and encompass transitions between ecozones.The Rio Grande serves as a natural border between the US State ofTexas and the Mexican States of Chihuahua, Coahuila, Nuevo León, andTamaulipas. Not only do millions of people live in this transboundary region, but also a substantial amount …

Resaca Ecosystem Development: Colonization And Succession Of The Macroinvertebrate Community, Leah M. Mcintosh

Theses and Dissertations - UTB/UTPA

Freshwater wetlands in the Lower Rio Grande Valley of Texas are locally known as resacas. Resacas are remnants of the Rio Grande River channel that were cut off by sedimentation and erosion of river banks. Many are maintained as permanent wetlands through intermittent water pumping from the river; and provide valuable habitat for fish, invertebrates, migratory birds and a diverse floral community in the semi-arid environment of South Texas. Three resacas in different stages of ecosystem development were studied, including one from day zero after re-flooding. The objectives were to document the colonization process of the re-flooded resaca and the …

A Comparison Of Fish Communities Over Different Reef Configurations In The Northwestern Gulf Of Mexico, Catheline Y. M. Froehlich

Theses and Dissertations - UTB/UTPA

High fishing pressure and limited natural habitat characterize the otherwise barren northwestern Gulf of Mexico. South Texas lacks extensive research of local natural and artificial reefs, and few studies report fish community and sportfish analysis of different reef densities. To alleviate this lack of data, fish abundances were monitored at different reef configurations in the PS-1047 Reef, 13 km off Port Mansfield, Texas, where 4000 concrete culverts were placed in 2011. Four culvert categories (CC1: 1-30 culverts, CC2: 31-70 culverts, CC3: 71-120 culverts, and CC4: 121-190 culverts in a 30-m radius), natural reefs and bare areas were sampled. Species abundances …

Metformin And Erlotinib Synergize To Inhibit Basal Breast Cancer, Ying-Ka Ingar Lau, Xing Du, Vinayak Rayannavar, Benjamin Hopkins, Jacquelyn Shaw, Eliana Bessler, Tiffany Thomas, Maira M. Pires, Megan Keniry

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Basal-like breast cancers (BBCs) are enriched for increased EGFR expression and decreased expression of PTEN. We found that treatment with metformin and erlotinib synergistically induced apoptosis in a subset of BBC cell lines. The drug combination led to enhanced reduction of EGFR, AKT, S6 and 4EBP1 phosphorylation, as well as prevented colony formation and inhibited mammosphere outgrowth. Our data with other compounds suggested that biguanides combined with EGFR inhibitors have the potential to outperform other targeted drug combinations and could be employed in other breast cancer subtypes, as well as other tumor types, with activated EGFR and PI3K signaling. Analysis …

Palo Alto Battlefield National Historical Park Breeding Bird Monitoring Data Fy 2014, Kenneth D. Pruitt

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Data are part of the GULN Inventory and Monitoring Program Land bird monitoring project for PAAL. Data were collected by Kenneth Pruit through a cooperative agreement with GULN and UT Brownsville in 2014. A separate reference is available for the Field Sheets that accompanies this data set.

https://irma.nps.gov/DataStore/Reference/Profile/2218507

Recruitment Patterns Of Juvenile Fish At An Artificial Reef In The Gulf Of Mexico, Rachel Noel Arney

Theses and Dissertations - UTB/UTPA

In 2011, the Texas Parks and Wildlife Department deployed 4,000 culverts as an artificial reef off Port Mansfield, TX to serve as habitat for sport fish species in the Gulf of Mexico. The aim of this study was to assess juvenile fish recruitment at particular culvert densities among the reef. Standard monitoring units for the recruitment of reef fish (SMURFs) were used in this study and acted as sampling devices. SMURFs were placed at thirteen sampling stations among four different reef patch densities and sampled repeatedly from 2013-2014. Culvert densities included stations with: zero culverts, 1-50 culverts, 51-100 culverts, and …

First Record Of The Tropical House Gecko Hemidactylus Mabouia (Moreau De Jonnès, 1818) In Texas, Alejandro Fierro-Cabo, August Rentfro

School of Earth, Environmental, & Marine Sciences Faculty Publications

Invasions of the tropical house gecko Hemidactylus mabouia (Moreau de Jonnes, 1818) in the tropical Americas are well documented. While this gecko has been reported as well established and expanding its range in Florida, here we document the first record in the United States outside of this state. We detected a small reproducing population in Cameron County, Brownsville, Texas. We expect a rapid colonization of buildings by this species in southern Texas. We consider that this potential invasion poses novel obstacles to the continued presence of the earlier invasive Mediterranean gecko Hemidactylus turcicus (Linnaeus, 1758) across Texas

Two Roads Converging: Mitochondria And Inflammatory Signaling, Robert Gilkerson, Luis Materon

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

As the complexity of cellular signaling in inflammatory response emerges, it is increasingly clear that mitochondria are directly involved in, and in some cases are even required for, activation of inflammatory response. As a bioenergetic organellar network, mitochondria dynamically modulate their organization and function in response to cellular signaling cues and metabolic demand. The NLRP3 inflammasome, a caspase-activating multifactor scaffolding assembly, is directly activated by mitochondrial factors and functional parameters. Mitochondria are also heavily implicated as downstream targets of inflammation in a variety of tissues. Elevated inflammation and cytokine-mediated damage to mitochondria are implicated in the pathogenesis of disparate conditions …

Management Of Silverleaf Whitefly, Bemisia Argentifolii Bellows And Perring (Hemiptera: Aleyrodidae), Using Non-Crop Companion Plants In Organically-Managed Cantaloupe Systems In South Texas, Ruth Renee Colyer

Theses and Dissertations - UTB/UTPA

Field trials in three separate phases of research were carried out from March, 2010, to August, 2012, to determine the effects of inter-cropping cantaloupe (Cucumis melo var. inodorus) with select non-crop companion plants in an effort to develop organic production strategies for melons in South Texas without the use of pesticides. A research program was designed and initiated to assess the effects of the companion plants and their ability to: 1) attract insect pollinators, and 2) repel or suppress pest species without affecting beneficial natural enemy complexes. The hypotheses were: 1) that organically-managed cantaloupe systems grown with non-crop companion plants …

Exposure To Traffic Pollution, Acute Inflammation And Autonomic Response In A Panel Of Car Commuters, Jeremy A. Sarnat, Rachel Golan, Roby Greenwald, Amit U. Raysoni, Priya Kewada, Andrea Winquist, Stefanie E. Sarnat, W. Dana Flanders, Maria C. Mirabelli, Jennifer E. Zora

School of Earth, Environmental, & Marine Sciences Faculty Publications

Background

Exposure to traffic pollution has been linked to numerous adverse health endpoints. Despite this, limited data examining traffic exposures during realistic commutes and acute response exists. Objectives: We conducted the Atlanta Commuters Exposures (ACE-1) Study, an extensive panel-based exposure and health study, to measure chemically-resolved in-vehicle exposures and corresponding changes in acute oxidative stress, lipid peroxidation, pulmonary and systemic inflammation and autonomic response.

Methods

We recruited 42 adults (21 with and 21 without asthma) to conduct two 2-h scripted highway commutes during morning rush hour in the metropolitan Atlanta area. A suite of in-vehicle particulate components were measured in …

Expression And Functional Characterization Of Mtp Genes From Arabidopsis Thaliana In Saccharomyces Cerevisiae, Yessica E. Cerino

Theses and Dissertations - UTB/UTPA

In this study the functional characterization and overexpression of Metal Tolerance Protein (MTP) cDNA were evaluated using a transgenic yeast (Saccharomyces cerevisiae) model system as a preliminary means for the characterization of metal tolerance conferred by putative Group III MTP genes from Arabidopsis thaliana. Metal disc assays for nickel, zinc, cobalt and cadmium treatments were performed to measure the tolerance imparted to yeast by the MTP cDNAs. Statistical analysis from zinc and cadmium assays showed AtMTP1 imparted the highest zinc tolerance in yeast, while AtMTPB1 imparted the highest cadmium and cobalt tolerance. However, AtMTPA2 and AtMTPB produced a decrease in …

Analysis Of High Fat Diet Induced Genes During Mammary Gland Development: Identifying Role Players In Poor Prognosis Of Breast Cancer, Raquel C. Martinez-Chacin, Megan Keniry, Robert Dearth

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Background

Epidemiological studies have shown that consumption of a high-fat diet (HFD) increases the risk of developing breast cancer (BC). Studies in rodents have shown HFD causes changes in the genetic programming of the maturing mammary gland (MG) increasing the susceptibility of developing the disease. Less is known about how HFD induced genes impact BC development. HFD exposure two weeks before conception to six weeks of age was previously shown to dramatically change MG gene expression in 10 week old mice. Therefore, we investigated these differentially expressed HFD-induced genes for their expression in BC using the NKI 295 breast tumor …

Acute Effects Of Whole Body Vibration Exercises With Various Frequencies And Amplitudes On Arterial Stiffness In Males, Omar Apodaca

Theses and Dissertations - UTB/UTPA

The purposes of the study were: 1) To examine the acute effects of dynamic whole body vibration (WBV) training on small and large arterial stiffness; 2) To examine the effects of different frequency and amplitude protocols in heart rate (HR), systolic and diastolic blood pressure, mean arterial pressure (MAP), cardiac output (CO), and stroke volume (SV); 3) Identify the best WBV protocol to decrease acutely arterial stiffness.

Isolation And Characterization Of Polychlorinated Biphenyl (Pcb) Degrading Bacteria Isolated From A Pcb Contaminated Reservoir In Donna, Tx, Sergio Cepeda

Theses and Dissertations - UTB/UTPA

The Donna Irrigation System (DIS) located in Donna, Hidalgo County, TX, was confirmed to be contaminated with polychlorinated biphenyls (PCBs) in 1993 and remediation of the site will be attempted in the future. The need to isolate novel PCB degrading bacteria for bioremediation has been an active area of research in recent decades; thus, it was hypothesized that a variety of microorganisms able to degrade PCBs could be isolated from sediments taken from the DIS. Two genes of the PCB degradation pathway, bphA1 and bphC, were detected in 43 bacterial isolates grown from DIS sediments using molecular and culture-based methodology, …

The Dominance Concept Inventory: A Tool For Assessing Undergraduate Student Alternative Conceptions About Dominance In Mendelian And Population Genetics, Joel K. Abraham, Kathryn E. Perez, Rebecca M. Price

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Despite the impact of genetics on daily life, biology undergraduates understand some key genetics concepts poorly. One concept requiring attention is dominance, which many students understand as a fixed property of an allele or trait and regularly conflate with frequency in a population or selective advantage. We present the Dominance Concept Inventory (DCI), an instrument to gather data on selected alternative conceptions about dominance. During development of the 16-item test, we used expert surveys (n = 12), student interviews (n = 42), and field tests (n = 1763) from introductory and advanced biology undergraduates at public …