Life Sciences Commons^™

Geographic Variation In Nrdna And Four Cpdna Regions Of Juniperus Excelsa: Analysis Of New Records From Bulgaria, Cyprus And Southwestern Turkey, Robert P. Adams, Salih Gucel, Tuğrul Mataraci, Alexander N. Tashev, Bouchra Douaihy, Magda Dou Dagher-Kharrat, Vahid Farzaliyev, Andrea E. Schwarzbach

Health & Biomedical Sciences Faculty Publications

Sequencing of nrDNA, plus four cp DNA regions: petN-psbM, trnS-trnG, trnD-trnT and trnL-trnF of newly acquired samples of J. excelsa from Bulgaria, Cyprus and Turkey showed little variation in J. excelsa (sensu stricto), except for the unusual situation in Lebanon, where J. excelsa and J. polycarpos (and likely J. p. var. turcomanica) grow near each other and may be hybridizing. The genetic composition of the eastern-most populations of J. excelsa in Turkey is unknown and deserves further study.

Modeled Inflow Validation & Nutrient Loading Estimation In Two Subwatersheds Of The Lower Laguna Madre, Hudson R. Deyoe, Warren Pulich, Nelun Fernando

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

University of Texas Rio Grande Valley will characterize nutrient loading rates into the Lower Laguna Madre for subwatersheds by monitoring stream flow and water quality (particularly total nitrogen and phosphorus). This information will be used to evaluate Texas Rainfall-Runoff model performance in estimating ungaged inflows and to establish a relationship between ungaged inflows and nutrient loading regimes to the Lower Laguna Madre. View on Map

Independent Test Assessment Using The Extreme Value Distribution Theory, Marcio Almeida, Lucy Blondell, Juan M. Peralta, Jack W. Kent Jr., Goo Jun, Tanya M. Teslovich, Christian Fuchsberger, Andrew R. Wood, Alisa K. Manning, Thomas D. Dyer, Ravindranath Duggirala, John Blangero

School of Medicine Publications

The new generation of whole genome sequencing platforms offers great possibilities and challenges for dissecting the genetic basis of complex traits. With a very high number of sequence variants, a naïve multiple hypothesis threshold correction hinders the identification of reliable associations by the overreduction of statistical power. In this report, we examine 2 alternative approaches to improve the statistical power of a whole genome association study to detect reliable genetic associations. The approaches were tested using the Genetic Analysis Workshop 19 (GAW19) whole genome sequencing data. The first tested method estimates the real number of effective independent tests actually being …

Chemical Flocculation For Removing Bentonite Spills In Water, James Jihoon Kang, Jacob D. Wiseman, Mckhenzy A. Welch, Richard A. Mclaughlin

School of Earth, Environmental, & Marine Sciences Faculty Publications

A potential environmental impact associated with horizontal directional drilling is the inadvertent return of bentonite-based drilling fluid to the surface via naturally occurring fractures or fissures. This study investigated a range of flocculants consisting of water-soluble linear polyacrylamides (PAMs) differing in charge (anionic, neutral, and cationic), biopolymer (chitosan) and gypsum for treating the bentonite suspension that might release with runoff or into stream water. Laboratory jar test were conducted with a 0.4 % (w/v) bentonite suspension having an average initial turbidity of 1,217 nephelometric turbidity units (NTU). None of the PAMs by themselves were effective in flocculating bentonite suspension ( …

Beyond Just Sea-Level Rise: Considering Macroclimatic Drivers Within Coastal Wetland Vulnerability Assessments To Climate Change, Michael J. Osland, Nicholas M. Enwright, Richard H. Day, Christopher A. Gabler, Camille L. Stagg, James B. Grace

School of Earth, Environmental, & Marine Sciences Faculty Publications

Due to their position at the land-sea interface, coastal wetlands are vulnerable to many aspects of climate change. However, climate change vulnerability assessments for coastal wetlands generally focus solely on sea-level rise without considering the effects of other facets of climate change. Across the globe and in all ecosystems, macroclimatic drivers (e.g., temperature and rainfall regimes) greatly influence ecosystem structure and function. Macroclimatic drivers have been the focus of climate change-related threat evaluations for terrestrial ecosystems, but largely ignored for coastal wetlands. In some coastal wetlands, changing macroclimatic conditions are expected to result in foundation plant species replacement, which would …

Horticultural Technique For Rearing And Redistribution Of The Sessile Biological Control Agent, Rhizaspidiotus Donacis On Its Host Plant, Arundo Donax, Joseph M. Villarreal, John A. Goolsby, Ann T. Vacek, Adalberto A. Perez De Leon, Alexis Racelis

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Arundo donax, giant reed is an invasive weed in the riparian habitats of the Rio Grande Basin. A biological control program using specialist insects from the native range in Mediterranean Europe, including the arundo scale, Rhi-zaspidiotus donacis has been implemented. The arundo scale is a sessile insect that spends most of its life cycle affixed to its host plant, thus creating challenges for its rearing and redistribution to field sites. A horticultural technique was developed which allowed for rearing of the scale on small, potted A. donax microplants, which could later be transplanted to field sites. Female scale reach maturity …

Engaging Minority Students In Sustainable Bioenergy And Water Quality Through An Education And Research Network, Krystel Castillo, Mauricio Cabrera-Rios, Michael W. Persans, Hudson R. Deyoe

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Growing energy demand is connected to water availability and climate change and it places additional stress on the environment. Thereby, It is critical to prepare the next generation of engineers and professionals to face the challenges in bioenergy, expand sustainable alternatives to fossil fuels1 and enable climate-smart agriculture2,3. To address this challenge, a career-oriented multidisciplinary educational model is being implemented at three minority-serving institutions. This paper discusses the foundation of this educational program, which provides a robust response to the current sustainability issues by conducting multidisciplinary coordinated education, mentoring, research and extension activities among multiple universities and laboratories. This educational …

Diabetes And Physical Activity, Jameela Banu

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Diabetes has been on the rise globally. Although many factors contribute to the increasing risk and manifestation of this disease lack and decreased physical activity/ exercise stands out as one of the major factors. Treatment interventions for prediabetic and diabetic patients include diet and lifestyle changes with enhanced physical activity/ exercise. Several types of physical activity are available for these patients but, recommendations have to be made on an individual basis after giving due consideration to the comorbidities and other risks and barriers. Implementation of progressive resistance therapy may be successful in maintaining glucose homeostasis in diabetic patients.

Two Complete Mitochondrial Genomes From Praticolella Mexicana Perez, 2011 (Polygyridae) And Gene Order Evolution In Helicoidea (Mollusca, Gastropoda), Russell L. Minton, Marco A. Martinez Cruz, Mark L. Farman, Kathryn E. Perez

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Helicoidea is a diverse group of land snails with a global distribution. While much is known regarding the relationships of helicoid taxa, comparatively little is known about the evolution of the mitochondrial genome in the superfamily. We sequenced two complete mitochondrial genomes from Praticolella mexicana Perez, 2011 representing the first such data from the helicoid family Polygyridae, and used them in an evolutionary analysis of mitogenomic gene order. We found the mitochondrial genome of P. mexicana to be 14,008 bp in size, possessing the typical 37 metazoan genes. Multiple alternate stop codons are used, as are incomplete stop codons. Mitogenome …

Impact Of The Biological Control Agent Tetramesa Romana (Hymenoptera: Eurytomidae) On Arundo Donax (Poaceae: Arundinoideae) Along The Rio Grande River In Texas, John A. Goolsby, Patrick J. Moran, Alexis Racelis, Kenneth R. Summy, Maricela Martinez Jimenez, Ronald D. Lacewell

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Five years post-release of the arundo gall wasp, Tetramesa romana, into the riparian habitats of the lower Rio Grande River, changes in the health of the invasive weed, Arundo donax, or giant reed, have been documented. These changes in plant attributes are fairly consistent along the study area of 558 river miles between Del Rio and Brownsville, TX, and support the hypothesis that the arundo wasp has had a significant impact as a biological control agent. Plant attributes were measured prior to release in 10 quadrats at each of 10 field sites in 2007, and measured again …

Collegiate Coaches’ Knowledge Of The Female Athlete Triad In Relation To Their Characteristics, Jillian E. Frideres, Sue G. Mottinger, Jose M. Palao

Health & Human Performance Faculty Publications

The purpose of this study was to determine what coaches of female athletes know about the female athlete triad. The sample involved 472 NCAA Division I coaches of female athletes. The variables studied were coaches’ knowledge and confidence about the triad and coaches’ characteristics (coach’s gender, age, type of coach, degree held, years of experience in coaching females, continuing education participation specific to the triad and/or triad components, and sport coached). Coaches’ knowledge of the female athlete triad is higher for coaches who have received triad education. No differences regarding knowledge of the female athlete triad were found by gender, …

A Variance Component Method For Integrated Pathway Analysis Of Gene Expression Data, Ellen E. Quillen, John Blangero, Laura Almasy

Human Genetics Publications

Background: The application of pathway and gene-set based analyses to high-throughput data is increasingly common and represents an effort to understand underlying biology where single-gene or single-marker analyses have failed. Many such analyses rely on the a priori identification of genes associated with the trait of interest. In contrast, this variance-component-based approach creates a similarity matrix of individuals based on the expression of genes in each pathway.

Methods: We compared 16 methods of calculating similarity for positive control matrices based on probes for the genes used to model the simulated Genetic Analysis Workshop phenotypes. Results: A simple correlation matrix outperforms …

Effects Of Shrub Encroachment And Removal On South Texas Coastal Prairie Flora, Parker Alex Watson

Theses and Dissertations

Watson, Parker Alex, Effects of shrub encroachment and removal on South Texas coastal prairie flora. Master of Science, December, 2015. 60 pages, 14 tables, 11 figures, 47 references, 5 appendices. Native-invasive honey mesquite (Prosopis glandulosa) and huisache (Acacia farnesiana) are aggressively encroaching onto South Texas coastal prairies, outcompeting Gulf cordgrass (Spartina spartinae) and associated prairie vegetation with potential legacy effects on the landscape. To measure conditions in the presence of shrubs, data loggers were installed for 16 mos in shrub understories and grass cover to monitor light, soil and air temperatures. Vegetation and canopy surveys were also conducted to evaluate …

Validating Side Scan Sonar As A Fish Survey Tool, Michael A. Bollinger

Theses and Dissertations

Hydroacoustic methods can be used to answer a variety of questions regarding fish populations and behavior. In this study, side scan sonar methodology was developed to quantify abundance and biomass and compared to established visual observation methods on SCUBA over artificial reef structures in the western Gulf of Mexico. Side scan sonar methods were equivalent to SCUBA surveys for measuring fish abundance over the same reef areas, however, abundances were significantly higher when the larger area sampled by side scan was utilized. Side scan sonar methods were also more time efficient than SCUBA, ROV and long line fishing methods (66.7%, …

Examination Of The Chagas Disease Vector, Triatoma Gerstackeri, In The Lower Rio Grande Valley, Alfredo Flores

Theses and Dissertations

Chagas disease is caused by the protozoan parasite Trypanosoma cruzi, which is carried by an insect vector of the family Reduviidae, and of the subfamily Triatomina. The species Triatoma gerstaeckeri is identified as the primary vector of T. cruzi within the Rio Grande Valley. Species of Triatoma gerstaeckeri were trapped in La Sal del Rey National Refuge in 2012 and 2013.

Data indicates a seasonal dispersal pattern of activity for T. gerstaeckeri. Optimal time of capture was between 1-2 hours after sunset. Different methods were utilized to determine the best combination of lures. Results indicate that the UV-light alone is …

Decomposition And Nitrogen Dynamics Of Turtle Grass (Thalassia Testudinum) In A Subtropical Estuarine System, Monica Delgado

Theses and Dissertations

Seagrass beds are pivotal in the functioning of coastal ecosystems in terms of productivity, organic matter turnover and nutrient cycling. Aiming to document decay and nitrogen (N) dynamics of turtle grass (Thalassia testudinum) in a subtropical estuarine system, decomposition patterns of leaves and rhizomes were characterized and compared. Nitrogen usage during decomposition of tissues, and of live tissues and epiphytes growing on live leaves, was also quantified and compared. Stable isotope ratios allowed tracing N within the seagrass bed, following N incorporation into seagrass tissues from the surrounding media (water, sediment). Leaves had a higher N content and decomposed at …

Activation Of Microglia In The Rotenone Lymnaea Stagnalis Model Of Parkinson's Disease, Xiomara Galvan

Theses and Dissertations

Parkinson’s disease (PD) is the most common irreversible neurodegenerative movement disorder affecting approximately ten million people worldwide. In the central nervous system (CNS), PD is known by the selective loss of Dopamine (DA) cells of the substantia nigra pars compacta. Although the disease has been very widely studied, the underlying mechanisms are not yet understood. Several hypotheses have arisen as result of injury of the mitochondria, some experiments include microglia activation, microtubule damage, and reactive oxidative species, which all result in the death of DA cells. The theory of microglia activation upon inflammation was explored in this study. Using the …

Balanus Eburneus (Crustacea: Cirripedia) As A Potential Indicator Of Estuarine System Recovery In South Texas: A Study Of Recruitment, Growth And Stable Isotopes, Crystal Martinez

Theses and Dissertations

Population characteristics of the barnacle Balanus eburneus were analyzed with the purpose of exploring its potential use as a discriminating indicator among sites exhibiting different disturbances or stages of recovery. The study was conducted from May 2012 to May 2013 at four estuaries in the Lower Laguna Madre, TX including a) Bahia Grande, currently under restoration and divided into Bahia Grande North and Bahia Grande South, b) San Martin Lake, which receives runoff water and c) South Bay, a relatively undisturbed reference estuary. In general, similar barnacle recruitment and growth trends in Bahia Grande South and South Bay were explained …

Comparing Reproductive Capacity Of Nearshore And Offshore Red Snapper, Lutjanus Campechanus, On Artificial Reefs In The Western Gulf Of Mexico, Ricky J. Alexander

Theses and Dissertations

Red snapper collected quarterly from four artificial reefs in south Texas were examined to compare total length, total weight, age, and the proportion of male to female red snapper, calculate gonadosomatic index values, batch fecundity, annual spawning frequency, and annual fecundity, and estimate size and age at maturity. Spawning occurred from April to September, with most active spawning observed in June (58%). Despite larger red snapper producing more eggs, and fish being larger offshore, most active spawning (68%) and egg production (74%) was observed nearshore. Female red snapper reached 50% maturity (L50) between 350-550 mm, and L75 by 600 mm. …

After More Than A Decade Of Soil Moisture Deficit, Tropical Rainforest Trees Maintain Photosynthetic Capacity, Despite Increased Leaf Respiration, Lucy Rowland, Raquel L. Lobo-Do-Vale, Bradley O. Christoffersen, Eliane A. Melém, Bart Kruijt, Steel S. Vasconcelos, Tomas Domingues, Oliver J. Binks, Alex A. R. Oliveira, Daniel Metcalfe

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Determining climate change feedbacks from tropical rainforests requires an understanding of how carbon gain through photosynthesis and loss through respiration will be altered. One of the key changes that tropical rainforests may experience under future climate change scenarios is reduced soil moisture availability. In this study we examine if and how both leaf photosynthesis and leaf dark respiration acclimate following more than 12 years of experimental soil moisture deficit, via a through-fall exclusion experiment (TFE) in an eastern Amazonian rainforest. We find that experimentally drought-stressed trees and taxa maintain the same maximum leaf photosynthetic capacity as trees in corresponding control …

Effects Of Control Release Fertilizers On Nutrient Leaching, Palm Growth And Production Cost, Pushpa Soti, Angie Fleurissaint, Stewart Reed, Krish Jayachandran

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Objective of this study was to evaluate the effect of different controlled release fertilizer technologies on nutrient leaching and plant growth parameters of two palm species, Chinese Fan (Livistona chinensis) and Queen (Syagrus romanzoffiana). We compared Nutri-Pak (12-4-12 controlled release packet) and Harrell’s (12-4-12 controlled release polymer coated urea) against Atlantic (8-4-12 controlled release polymer coated urea, coated sulfate of potash), the most commonly used palm fertilizer in South Florida. Plants were grown in 25 cm (11 L) pots under 70% shade, watered weekly, with pest and weed control done as required. Plant growth parameters: number of leaves, leaf length …

Effect Of Binder On The Physical Stability And Bactericidal Property Of Titanium Dioxide (Tio2) Nanocoatings On Food Contact Surfaces, Veerachandra K. Yemmireddy, Yen-Con Hung

School of Earth, Environmental, & Marine Sciences Faculty Publications

TiO2 is a promising photocatalyst for use in food processing environment as an antimicrobial coating. The purpose of this study was to determine the effect of different binding agents on the physical stability and bactericidal property of TiO2 nanocoatings created on stainless steel surfaces. A total of six different coating suspensions were prepared by mixing TiO2 (Aeroxide® P-25) nanoparticles (NPs) with three different types of binders (Shellac (A), polyuretahne (B), and polycrylic (C)) at a 1:4 to 1:16 NP to binder weight ratio. Bactericidal activity of these TiO2 coatings against Escherichia coli O157:H7 (5-strain) was determined at three different UV-A …

Genomic And Transcriptomic Evidence For Scavenging Of Diverse Organic Compounds By Widespread Deep-Sea Archaea, Meng Li, Brett J. Baker, Karthik Anantharaman, Sunit Jain, John A. Breier, Gregory J. Dick

School of Earth, Environmental, & Marine Sciences Faculty Publications

Microbial activity is one of the most important processes to mediate the flux of organic carbon from the ocean surface to the seafloor. However, little is known about the microorganisms that underpin this key step of the global carbon cycle in the deep oceans. Here we present genomic and transcriptomic evidence that five ubiquitous archaeal groups actively use proteins, carbohydrates, fatty acids and lipids as sources of carbon and energy at depths ranging from 800 to 4,950 m in hydrothermal vent plumes and pelagic background seawater across three different ocean basins. Genome-enabled metabolic reconstructions and gene expression patterns show that …

A Global Perspective On The Influence Of Environmental Exposures On The Nervous System, Desire Tshala-Katumbay, Jean-Claude Mwanza, Diane S. Rohlman, Gladys E. Maestre, Reinaldo B. Oria

School of Medicine Publications

Economic transitions in the era of globalization warrant a fresh look at the neurological risks associated with environmental change. These are driven by industrial expansion, transfer and mobility of goods, climate change and population growth. In these contexts, risk of infectious and non-infectious diseases are shared across geographical boundaries. In low- and middle-income countries, the risk of environmentally mediated brain disease is augmented several fold by lack of infrastructure, poor health and safety regulations, and limited measures for environmental protection. Neurological disorders may occur as a result of direct exposure to chemical and/or non-chemical stressors, including but not limited to, …

The Transcriptional Landscape Of Age In Human Peripheral Blood, Marjolein J. Peters, Roby Joehanes, Luke C. Pilling, Claudia Schurmann, Karen N. Conneely, Harald Hh Goring, Joanne E. Curran, Sarah Williams-Blangero, John Blangero

School of Medicine Publications

Disease incidences increase with age, but the molecular characteristics of ageing that lead to increased disease susceptibility remain inadequately understood. Here we perform a whole-blood gene expression meta-analysis in 14,983 individuals of European ancestry (including replication) and identify 1,497 genes that are differentially expressed with chronological age. The age-associated genes do not harbor more age-associated CpG-methylation sites than other genes, but are instead enriched for the presence of potentially functional CpG-methylation sites in enhancer and insulator regions that associate with both chronological age and gene expression levels. We further used the gene expression profiles to calculate the 'transcriptomic age' of …

Ca2+/Calmodulin Regulates Kvβ1.1-Mediated Inactivation Of Voltage-Gated K+ Channels, Sandip M. Swain, Nirakar Sahoo, Sophie Dennhardt, Roland Schönherr, Stefan H. Heinemann

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

A-type K+ channels open on membrane depolarization and undergo subsequent rapid inactivation such that they are ideally suited for fine-tuning the electrical signaling in neurons and muscle cells. Channel inactivation mostly follows the so-called ball-and-chain mechanism, in which the N-terminal structures of either the K+ channel’s α or β subunits occlude the channel pore entry facing the cytosol. Inactivation of Kv1.1 and Kv1.4 channels induced by Kvβ1.1 subunits is profoundly decelerated in response to a rise in the intracellular Ca2+ concentration, thus making the affected channel complexes negative feedback regulators to limit neuronal overexcitation. With electrophysiological and biochemical experiments we …

Using Fish Population Metrics To Compare The Effects Of Artificial Reef Density, Catheline Y. M. Froehlich, Richard Kline

School of Earth, Environmental, & Marine Sciences Faculty Publications

Artificial reefs continue to be added as habitat throughout the world, yet questions remain about how reef design affects fish diversity and abundance. In the present study, the effects of reef density were assessed for fish communities and sizes of economically valuable Lutjanus campechanus 13 km off Port Mansfield, Texas, at a reef composed of more than 4000 concrete culverts. The study spanned from May to June in 2013 and 2014, and sites sampled included natural reefs, bare areas, and varying culvert patch density categories, ranging from 1–190 culverts. Abundances of adults and species evenness of juvenile populations differed between …

A Bomb Set To Drop: Parthenogenetic Marmorkrebs For Sale In Ireland, A European Location Without Non-Indigenous Crayfish, Zen Faulkes

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Ireland is one of the few locations in Europe where non-indigenous North American crayfish species have not been introduced, and is a refuge for endangered white-clawed crayfish, Austropotamobius pallipes (Lereboullet, 1858). The parthenogenetic crayfish species Marmorkrebs, Procambarus fallax f. virginalis (Hagen, 1870), is sold in the pet trade in Ireland within the recorded range of A. pallipes. Marmorkrebs risk being introduced into Irish waters, where they could threaten A. pallipes populations, particularly as a vector for crayfish plague.

Analysis Of Variables Related To Corridor Use By Ocelots And Bobcats In South Texas, Sarah E. Nordlof

Theses and Dissertations - UTB/UTPA

Biologists estimate that less than 50 endangered ocelots (Leopardus pardalis) remain in the United States, restricted to two small populations in Cameron and Willacy Counties located in deep south Texas. Conversely, bobcats (Lynx rufus) are abundant in south Texas; however, two of the biggest threats to both species are vehicle collisions and habitat fragmentation. To mitigate these threats, the installation of wildlife crossings has been proposed to decrease the number of road mortalities, and wildlife corridors have been suggested as a useful tool for providing increased habitat connectivity. However, research on ocelot use of corridors and wildlife crossings in Texas …

Infection Rates And Distribution Of Trypanosoma Cruzi In Triatomine Insects From Several Public Parks Of Starr, Hidalgo And Cameron Counties, Carlos Guzman Jr.

Theses and Dissertations - UTB/UTPA

Chagas disease is caused by the protozoan Trypanosoma cruzi and is a major public health concern in many areas of the world, including the United States. The disease is transmitted by insect vectors known as kissing bugs from the subfamily Triatominae. While the majority of studies focus on domestic and peri-domestic collections, this study collected insect vectors from state parks in the Lower Rio Grande Valley. PCR analysis was done to obtain infection rates for collected insects, and a morphological examination was done to check insects for gender. In total 18 insects were captured with 12 of the captured insects …