Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons™

Open Access. Powered by Scholars. Published by Universities.®

University of South Florida

Discipline
Keyword
Publication Year
Publication
Publication Type
File Type

Articles 931 - 960 of 2279

Full-Text Articles in Life Sciences

Large-Scale Intrusion Of Circumpolar Deep Water On Antarctic Margin Recorded By Stylasterid Corals, Theresa M. King, Brad E. Rosenheim, Alexandra L. Post, Theresa Gabris, Taylor Burt, Eugene W. Domack Jan 2018

Large-Scale Intrusion Of Circumpolar Deep Water On Antarctic Margin Recorded By Stylasterid Corals, Theresa M. King, Brad E. Rosenheim, Alexandra L. Post, Theresa Gabris, Taylor Burt, Eugene W. Domack

Marine Science Faculty Publications

We present centennial-scale radiocarbon (14C) records archived by deep sea stylasterid corals from the outer shelf and upper slope of the Antarctic margin. These novel stylasterids (Errina spp.) were collected from the western Ross Sea shelf (500 m) and slope (1,700 m), as well as the eastern Wilkes Land shelf (670 m). We provide two corals from each region and document an abrupt reversal of 14C ages in the upper (younger) part of each coral. We test the statistical robustness of each record and demonstrate the significance of the age reversals, as well as the ability …

Go to article

Claw Morphometrics In Monitor Lizards: Variable Substrate And Habitat Use Correlate To Shape Diversity Within A Predator Guild, Domenic C. D'Amore, Simon Clulow, J. Sean Doody, David Rhind, Colin R. Mchenry Jan 2018

Claw Morphometrics In Monitor Lizards: Variable Substrate And Habitat Use Correlate To Shape Diversity Within A Predator Guild, Domenic C. D'Amore, Simon Clulow, J. Sean Doody, David Rhind, Colin R. Mchenry

USF St. Petersburg Campus Faculty Publications

Numerous studies investigate morphology in the context of habitat, and lizards have received particular attention. Substrate usage is often reflected in the morphology of characters associated with locomotion, and, as a result, claws have become well-studied ecomorphological traits linking the two. The Kimberley predator guild of Western Australia consists of 10 sympatric varanid species. The purpose of this study was to quantify claw size and shape in the guild using geometric morphometrics, and determine whether these features correlated with substrate use and habitat. Each species was assigned a Habitat/substrate group based on the substrate their claws interact with in their …

Go to article

The Ecology And Economics Of Restoration: When, What, Where, And How To Restore Ecosystems, Jason R. Rohr, Emily S. Bernhardt, Marc W. Cadotte, William H. Clements Jan 2018

The Ecology And Economics Of Restoration: When, What, Where, And How To Restore Ecosystems, Jason R. Rohr, Emily S. Bernhardt, Marc W. Cadotte, William H. Clements

Integrative Biology Faculty and Staff Publications

Restoration ecology has provided a suite of tools for accelerating the recovery of ecosystems damaged by drivers of global change. We review both the ecological and economic concepts developed in restoration ecology, and offer guidance on when, what, where, and how to restore ecosystems. For when to restore, we highlight the value of pursuing restoration early to prevent ecosystems from crossing tipping points and evaluating whether unassisted natural recovery is more cost-effective than active restoration. For what to restore, we encourage developing a restoration plan with stakeholders that will restore structural, compositional, and functional endpoints, and whose goal is a …

Go to article

A Faculty Professional Development Model That Improves Student Learning, Encourages Active-Learning Instructional Practices, And Works For Faculty At Multiple Institutions, Karen N. Pelletrau, Jennifer K. Knight, Paula P. Lemons, Jill S. Mccourt, John E, Merrill, Ross H. Nehm, Luanna B Prevost, Mark Urban-Lurain, Michelle K. Smith Jan 2018

A Faculty Professional Development Model That Improves Student Learning, Encourages Active-Learning Instructional Practices, And Works For Faculty At Multiple Institutions, Karen N. Pelletrau, Jennifer K. Knight, Paula P. Lemons, Jill S. Mccourt, John E, Merrill, Ross H. Nehm, Luanna B Prevost, Mark Urban-Lurain, Michelle K. Smith

Integrative Biology Faculty and Staff Publications

Helping faculty develop high-quality instruction that positively affects student learning can be complicated by time limitations, a lack of resources, and inexperience using student data to make iterative improvements. We describe a community of 16 faculty from five institutions who overcame these challenges and collaboratively designed, taught, iteratively revised, and published an instructional unit about the potential effect of mutations on DNA replication, transcription, and translation. The unit was taught to more than 2000 students in 18 courses, and student performance improved from preassessment to postassessment in every classroom. This increase occurred even though faculty varied in their instructional practices …

Go to article

Resilience In Moving Water: Effects Of Turbulence On The Predatory Impact Of The Lobate Ctenophore Mnemiopsis Leidyi, Cornelia Jaspers, John H. Costello, Kelly R. Sutherland, Brad J. Gemmell, Kelsey N. Lucas, Jennifer Tackett, Kara Dodge, Sean P. Colin Jan 2018

Resilience In Moving Water: Effects Of Turbulence On The Predatory Impact Of The Lobate Ctenophore Mnemiopsis Leidyi, Cornelia Jaspers, John H. Costello, Kelly R. Sutherland, Brad J. Gemmell, Kelsey N. Lucas, Jennifer Tackett, Kara Dodge, Sean P. Colin

Integrative Biology Faculty and Staff Publications

Despite its delicate morphology, the lobate ctenophore Mnemiopsis leidyi thrives in coastal ecosystems as an influential zooplankton predator. Coastal ecosystems are often characterized as energetic systems with high levels of natural turbulence in the water column. To understand how natural wind‐driven turbulence affects the feeding ecology of M. leidyi, we used a combination of approaches to quantify how naturally and laboratory generated turbulence affects the behavior, feeding processes and feeding impact of M. leidyi. Experiments using laboratory generated turbulence demonstrated that turbulence can reduce M. leidyi feeding rates on copepods and Artemia nauplii by > 50%. However, detailed feeding …

Go to article

Unprecedented Diversity Of Ssdna Phages From The Family Microviridae Detected Within The Gut Of A Protochordate Model Organism (Ciona Robusta), Alexandria Creasy, Karyna Rosario, Brittany Leigh, Larry Dishaw, Mya Breitbart Jan 2018

Unprecedented Diversity Of Ssdna Phages From The Family Microviridae Detected Within The Gut Of A Protochordate Model Organism (Ciona Robusta), Alexandria Creasy, Karyna Rosario, Brittany Leigh, Larry Dishaw, Mya Breitbart

Marine Science Faculty Publications

Phages (viruses that infect bacteria) play important roles in the gut ecosystem through infection of bacterial hosts, yet the gut virome remains poorly characterized. Mammalian gut viromes are dominated by double-stranded DNA (dsDNA) phages belonging to the order Caudovirales and single-stranded DNA (ssDNA) phages belonging to the family Microviridae. Since the relative proportion of each of these phage groups appears to correlate with age and health status in humans, it is critical to understand both ssDNA and dsDNA phages in the gut. Building upon prior research describing dsDNA viruses in the gut of Ciona robusta, a marine invertebrate model system …

Go to article

Genome Sequence Of Pm2-Like Phage Cr39582, Induced From A Pseudoalteromonas Sp. Isolated From The Gut Of Ciona Robusta, Brittany Leigh, Mya Breitbart, Hanna Oksanen, Dennis Bamford, Larry Dishaw Jan 2018

Genome Sequence Of Pm2-Like Phage Cr39582, Induced From A Pseudoalteromonas Sp. Isolated From The Gut Of Ciona Robusta, Brittany Leigh, Mya Breitbart, Hanna Oksanen, Dennis Bamford, Larry Dishaw

Marine Science Faculty Publications

Phage Cr39582 was induced by mitomycin C from Pseudoalteromonas sp. strain Cr6751, isolated from a marine invertebrate gut. Pseudoalteromonas phage Cr39582 has 85% pairwise nucleotide identity with phage PM2 but lacks sequence homology in the spike protein. This report supports previous bioinformatic identification of corticoviral sequences within aquatic bacterial genomes.

Go to article

Elementary Student Outreach Activity Demonstrating The Use Of Phage Therapy Heroes To Combat Bacterial Infections, Mya Breitbart, Kema Malki, Natalie Sawaya, Chelsea Bonnain, Mark Martin Jan 2018

Elementary Student Outreach Activity Demonstrating The Use Of Phage Therapy Heroes To Combat Bacterial Infections, Mya Breitbart, Kema Malki, Natalie Sawaya, Chelsea Bonnain, Mark Martin

Marine Science Faculty Publications

No abstract provided.

Go to article

Discovery Of Four Novel Circular Single-Stranded Dna Viruses In Fungus-Farming Termites, Mason Kerr, Karyna Rosario, Christopher C. M. Baker, Mya Breitbart Jan 2018

Discovery Of Four Novel Circular Single-Stranded Dna Viruses In Fungus-Farming Termites, Mason Kerr, Karyna Rosario, Christopher C. M. Baker, Mya Breitbart

Marine Science Faculty Publications

Here, we describe four novel circular single-stranded DNA viruses discovered in fungus-farming termites (Odontotermes sp.). The viruses, named termite-associated circular virus 1 (TaCV-1) through TaCV-4, are most similar to members of the family Genomoviridae and were widely detected in African termite mounds.

Go to article

Evaluation Of Marine Zooplankton Community Structure Through Environmental Dna Metabarcoding, Anni Djurhuus, Kathleen Pitz, Natalie Sawaya, Jaimie Rojas‐Márquez, Brianna Michaud, Enrique Montes, Frank E. Muller-Karger, Mya Breitbart Jan 2018

Evaluation Of Marine Zooplankton Community Structure Through Environmental Dna Metabarcoding, Anni Djurhuus, Kathleen Pitz, Natalie Sawaya, Jaimie Rojas‐Márquez, Brianna Michaud, Enrique Montes, Frank E. Muller-Karger, Mya Breitbart

Marine Science Faculty Publications

Zooplankton dominate the abundance and biomass of multicellular animals in pelagic marine environments; however, traditional methods to characterize zooplankton communities are invasive and laborious. This study compares zooplankton taxonomic composition revealed through metabarcoding of the cytochrome oxidase I (COI) and 18S rRNA genes to traditional morphological identification by microscopy. Triplicates of three different sample types were collected from three coral reef sites in the Florida Keys National Marine Sanctuary: (1) 1 L surface seawater samples prefiltered through 3 μm filters and subsequently collected on 0.22 μm filters for eDNA (PF‐eDNA); (2) 1 L surface seawater samples filtered on …

Go to article

Linking Capacity Development To Goos Monitoring Networks To Achieve Sustained Ocean Observation, Frank E. Muller-Karger Jan 2018

Linking Capacity Development To Goos Monitoring Networks To Achieve Sustained Ocean Observation, Frank E. Muller-Karger

Marine Science Faculty Publications

Developing enduring capacity to monitor ocean life requires investing in people and their institutions to build infrastructure, ownership, and long-term support networks. International initiatives can enhance access to scientific data, tools and methodologies, and develop local expertise to use them, but without ongoing engagement may fail to have lasting benefit. Linking capacity development and technology transfer to sustained ocean monitoring is a win-win proposition. Trained local experts will benefit from joining global communities of experts who are building the comprehensive Global Ocean Observing System (GOOS). This two-way exchange will benefit scientists and policy makers in developing and developed countries. The …

Go to article

Water Quality Drivers In 11 Gulf Of Mexico Estuaries, Matthew J. Mccarthy, Daniel Otis, Pablo Méndez-Lázaro, Frank Muller-Karger Jan 2018

Water Quality Drivers In 11 Gulf Of Mexico Estuaries, Matthew J. Mccarthy, Daniel Otis, Pablo Méndez-Lázaro, Frank Muller-Karger

Marine Science Faculty Publications

Coastal water-quality is both a primary driver and also a consequence of coastal ecosystem health. Turbidity, a measure of dissolved and particulate water-quality matter, is a proxy for water quality, and varies on daily to interannual periods. Turbidity is influenced by a variety of factors, including algal particles, colored dissolved organic matter, and suspended sediments. Identifying which factors drive trends and extreme events in turbidity in an estuary helps environmental managers and decision makers plan for and mitigate against water-quality issues. Efforts to do so on large spatial scales have been hampered due to limitations of turbidity data, including coarse …

Go to article

Seasonal Patterns In Phytoplankton Biomass Across The Northern And Deep Gulf Of Mexico: A Numerical Model Study, Fabian A. Gomez, Sang-Ki Lee, Frank J. Hernandez Jr, Frank E. Muller-Karger, John T. Lamkin Jan 2018

Seasonal Patterns In Phytoplankton Biomass Across The Northern And Deep Gulf Of Mexico: A Numerical Model Study, Fabian A. Gomez, Sang-Ki Lee, Frank J. Hernandez Jr, Frank E. Muller-Karger, John T. Lamkin

Marine Science Faculty Publications

Biogeochemical models that simulate realistic lower-trophic-level dynamics, including the representation of main phytoplankton and zooplankton functional groups, are valuable tools for improving our understanding of natural and anthropogenic disturbances in marine ecosystems. Previous three-dimensional biogeochemical modeling studies in the northern and deep Gulf of Mexico (GoM) have used only one phytoplankton and one zooplankton type. To advance our modeling capability of the GoM ecosystem and to investigate the dominant spatial and seasonal patterns of phytoplankton biomass, we configured a 13-component biogeochemical model that explicitly represents nanophytoplankton, diatoms, micro-, and mesozooplankton. Our model outputs compare reasonably well with observed patterns in …

Go to article

Rapid Coastal Forest Decline In Florida's Big Bend, Matthew J. Mccarthy, Benjamin Dimmitt, Frank E. Muller-Karger Jan 2018

Rapid Coastal Forest Decline In Florida's Big Bend, Matthew J. Mccarthy, Benjamin Dimmitt, Frank E. Muller-Karger

Marine Science Faculty Publications

Coastal ecosystems throughout the world are increasingly vulnerable to degradation as a result of accelerating sea-level rise and saltwater intrusion, more frequent and powerful extreme weather events, and anthropogenic impacts. Hardwood swamp forests in the Big Bend region of Florida’s Gulf of Mexico coast (USA) are largely devoid of the latter, but have degraded rapidly since the turn of the 21st Century. Photographs of the forest, collected on the ground since 2009, were used to guide an analysis of a 60 km2 study area using satellite images. The images confirm that the coastal forest area declined 0.60% from 1982 …

Go to article

Impacts Of 40 Years Of Land Cover Change On Water Quality In Tampa Bay, Florida, Matthew J. Mccarthy, Frank E. Muller-Karger, Daniel Otis, Pablo Méndez-Lázaro Jan 2018

Impacts Of 40 Years Of Land Cover Change On Water Quality In Tampa Bay, Florida, Matthew J. Mccarthy, Frank E. Muller-Karger, Daniel Otis, Pablo Méndez-Lázaro

Marine Science Faculty Publications

Land cover changes in the Tampa Bay watershed (Florida) over the past four decades were examined along with precipitation and wind observations to help understand causes of long-term changes in turbidity and chlorophyll concentration within the Tampa Bay estuary. Water quality showed a statistically significant relationship to land cover fraction in the watershed compared to long-term precipitation or wind stress. Redundancy Analyses with Akaike’s Information Criterion and non-parametric multiple regressions determined that turbidity and chlorophyll concentration decreased bay-wide from 1974–2012 with increased developed land fraction (R2 > 0.75, p-value < 0.05). Various segments of the estuary showed different significant responses to developed land (R2 > 0.75, p-value < 0.05), agricultural land (R2 > 0.93, p-value < 0.02), bare land (R2 = 0.77, p-value = 0.001), and wind stress (R2 = 0.91, p-value = 0.04) at different times of year.

Go to article

Advancing Marine Biological Observations And Data Requirements Of The Complementary Essential Ocean Variables (Eovs) And Essential Biodiversity Variables (Ebvs) Frameworks, Frank E. Muller-Karger, Enrique Montes Jan 2018

Advancing Marine Biological Observations And Data Requirements Of The Complementary Essential Ocean Variables (Eovs) And Essential Biodiversity Variables (Ebvs) Frameworks, Frank E. Muller-Karger, Enrique Montes

Marine Science Faculty Publications

Measurements of the status and trends of key indicators for the ocean and marine life are required to inform policy and management in the context of growing human uses of marine resources, coastal development, and climate change. Two synergistic efforts identify specific priority variables for monitoring: Essential Ocean Variables (EOVs) through the Global Ocean Observing System (GOOS), and Essential Biodiversity Variables (EBVs) from the Group on Earth Observations Biodiversity Observation Network (GEO BON) (see Data Sheet 1 in Supplementary Materials for a glossary of acronyms). Both systems support reporting against internationally agreed conventions and treaties. GOOS, established under the auspices …

Go to article

Description And Mechanisms Of The Mid-Year Upwelling In The Southern Caribbean Sea From Remote Sensing And Local Data, Digna T. Rueda-Roa, Tal Ezer, Frank E. Muller-Karger Jan 2018

Description And Mechanisms Of The Mid-Year Upwelling In The Southern Caribbean Sea From Remote Sensing And Local Data, Digna T. Rueda-Roa, Tal Ezer, Frank E. Muller-Karger

Marine Science Faculty Publications

The southern Caribbean Sea experiences strong coastal upwelling between December and April due to the seasonal strengthening of the trade winds. A second upwelling was recently detected in the southeastern Caribbean during June-August, when local coastal wind intensities weaken. Using synoptic satellite measurements and in situ data, this mid-year upwelling was characterized in terms of surface and subsurface temperature structures, and its mechanisms were explored. The mid-year upwelling lasts 6-9 weeks with satellite sea surface temperature (SST) ~1-2° C warmer than the primary upwelling. Three possible upwelling mechanisms were analyzed: cross-shore Ekman transport (csET) due to alongshore winds, wind curl …

Go to article

Ecosystem Model Of The Entire Beaufort Sea Marine Ecosystem: A Temporal Tool For Assessing Food-Web Structure And Marine Animal Populations From 1970 To 2014, Paul M. Suprenand, Cameron H. Ainsworth, Carie Hoover Jan 2018

Ecosystem Model Of The Entire Beaufort Sea Marine Ecosystem: A Temporal Tool For Assessing Food-Web Structure And Marine Animal Populations From 1970 To 2014, Paul M. Suprenand, Cameron H. Ainsworth, Carie Hoover

Marine Science Faculty Publications

The Beaufort Sea coastal-marine ecosystem is a 476,000 km2 area in the Arctic Ocean, which extends from -112.5 to -158.0° longitude to 67.5 to 75.0° latitude. Within this polar area the United States indigenous communities of Barrow, Kaktovik, and Nuiqsut, and the Canadian indigenous communities of Aklavik, Inuvik, Tuktoyaktuk, Paulatuk, Ulukhaktok, and Sachs Harbour, subsist by harvesting marine mammals, fish, and invertebrates to provide the majority of their community foods. The Beaufort Sea coastal-marine ecosystem includes many specialized marine animals whose life history is tied to the sea ice, such as polar bears that rely on sea-ice for foraging activities …

Go to article

Ecosystem Models Of Lake Victoria (East Africa): Exploring The Sensitivity Of Ecosystem Effects Of Fishing To Model Choice, Vianny Natugonza, Cameron Ainsworth, Erla Sturludóttir, Laban Musinguzi, Richard Ogutu-Ohwayo, Tumi Tomasson, Chrisphine Nyamweya, Gunnar Stefansson Jan 2018

Ecosystem Models Of Lake Victoria (East Africa): Exploring The Sensitivity Of Ecosystem Effects Of Fishing To Model Choice, Vianny Natugonza, Cameron Ainsworth, Erla Sturludóttir, Laban Musinguzi, Richard Ogutu-Ohwayo, Tumi Tomasson, Chrisphine Nyamweya, Gunnar Stefansson

Marine Science Faculty Publications

Ecosystem simulation models are valuable tools for strengthening and promoting ecosystem-based fisheries management (EBFM). However, utility of these models in practical fisheries management is often undermined by lack of simple means to test the effect of uncertainty on model outputs. Recently, the use of multiple ecosystem models has been recommended as an ‘insurance’ against effects of uncertainty that comes with modelling complex systems. The assumption is that if models with different structure and formulation give consistent results, then, policy prescriptions are robust (i.e. less sensitive to model choice). However, information on the behaviour of trends from structurally-distinct ecosystem models with …

Go to article

Producing Distribution Maps For A Spatially-Explicit Ecosystem Model Using Large Monitoring And Environmental Databases And A Combination Of Interpolation And Extrapolation, Arnaud Grüss, Michael D. Drexler, Cameron H. Ainsworth, Elizabeth A. Babcock, Joseph H. Tarnecki, Matthew S. Love Jan 2018

Producing Distribution Maps For A Spatially-Explicit Ecosystem Model Using Large Monitoring And Environmental Databases And A Combination Of Interpolation And Extrapolation, Arnaud Grüss, Michael D. Drexler, Cameron H. Ainsworth, Elizabeth A. Babcock, Joseph H. Tarnecki, Matthew S. Love

Marine Science Faculty Publications

To be able to simulate spatial patterns of predator-prey interactions, many spatially-explicit ecosystem modeling platforms, including Atlantis, need to be provided with distribution maps defining the annual or seasonal spatial distributions of functional groups and life stages. We developed a methodology combining extrapolation and interpolation of the predictions made by statistical habitat models to produce distribution maps for the fish and invertebrates represented in the Atlantis model of the Gulf of Mexico (GOM) Large Marine Ecosystem (LME) (“Atlantis-GOM”). This methodology consists of: (1) compiling a large monitoring database, gathering all the fisheries-independent and fisheries-dependent data collected in the northern (U.S.) …

Go to article

Remote Sensing As A Monitoring And Modeling Tool, Jennifer Cannizzaro, Inia Soto, Chuanmin Hu Jan 2018

Remote Sensing As A Monitoring And Modeling Tool, Jennifer Cannizzaro, Inia Soto, Chuanmin Hu

Marine Science Faculty Publications

No abstract provided.

Go to article

Global Water Clarity: Continuing A Century-Long Monitoring, Z. Lee, R. Arnone, D. Boyce, B. Franz, S. Greb, C. Hu, S. Lavender, M. Lewis, B. Schaeffer, S. Shang, M. Wang, M. Wernand, C. Wilson Jan 2018

Global Water Clarity: Continuing A Century-Long Monitoring, Z. Lee, R. Arnone, D. Boyce, B. Franz, S. Greb, C. Hu, S. Lavender, M. Lewis, B. Schaeffer, S. Shang, M. Wang, M. Wernand, C. Wilson

Marine Science Faculty Publications

An approach that combines field observations and satellite inferences of Secchi depth could transform how we assess water clarity across the globe and pinpoint key changes over the past century.

Go to article

Can Modis Land Reflectance Products Be Used For Estuarine And Inland Waters?, Lian Feng, Chuanmin Hu, Junsheng Li Jan 2018

Can Modis Land Reflectance Products Be Used For Estuarine And Inland Waters?, Lian Feng, Chuanmin Hu, Junsheng Li

Marine Science Faculty Publications

Although designed for land surfaces, MODIS Aqua surface reflectance data products (MYD09, termed as R_Land in this work) have also been used for water applications. Yet to date their uncertainties and general suitability in such applications have rarely been documented. In this study, R_Land products of two regions (Chesapeake Bay and Taihu Lake) between July 2002 and December 2015 are evaluated against in situ measurements and against reflectance products derived by the MODIS Ocean Team using atmospheric correction schemes specifically designed for water applications, namely the default atmospheric correction method based on the near-infrared (NIR) bands (denoted as R_NIR, data …

Go to article

A Color-Index-Based Empirical Algorithm For Determining Particulate Organic Carbon Concentration In The Ocean From Satellite Observations, Chengfeng Le, Xueying Zhou, Chuanmin Hu, Zhongping Lee, Lin Li, Dariusz Stramski Jan 2018

A Color-Index-Based Empirical Algorithm For Determining Particulate Organic Carbon Concentration In The Ocean From Satellite Observations, Chengfeng Le, Xueying Zhou, Chuanmin Hu, Zhongping Lee, Lin Li, Dariusz Stramski

Marine Science Faculty Publications

An empirical algorithm for estimating particulate organic carbon (POC) concentration in the surface ocean from satellite observations is formulated and validated using in situ POC data and remote-sensing reflectance (Rrs) data obtained from match-up satellite ocean color measurements. The algorithm builds upon the band-difference algorithm concept, which was originally developed for estimating chlorophyll-a concentration in clear waters. This algorithm utilizes three spectral bands centered approximately at 490, 550, and 670 nm to determine a color index (CIPOC), from which POC can be estimated from satellite measurements. For comparison, the blue-green band-ratio algorithm is also formulated …

Go to article

Incorporation Of Acoustic Sensors On A Coastal Ocean Monitoring Platform For Measurements Of Biological Activity, James Locascio, David Mann, Kyle Wilcox, Mark Luther Jan 2018

Incorporation Of Acoustic Sensors On A Coastal Ocean Monitoring Platform For Measurements Of Biological Activity, James Locascio, David Mann, Kyle Wilcox, Mark Luther

Marine Science Faculty Publications

Ocean observing stations have mainly focused on data collection of physical parameters measured in the ocean and atmosphere and also, to some extent, of biogeochemical parameters. Robust sensors capable of measuring biological data reflective of higher tropic level function at the same time scales as other parameters are not yet commonly incorporated into the sensor array used on observing platforms. In this project, we reengineered a coastal ocean observatory to include two hydrophones for this purpose. One hydrophone (HTI 96-MIN) was used to record ambient acoustic signals of fish reproductive sounds, and a second hydrophone (Vemco VR2C) was used to …

Go to article

Expression And Function Of Nuclear Receptor Coactivator 4 Isoforms In Transformed Endometriotic And Malignant Ovarian Cells, Stephanie Rockfield, Idhaliz Flores, Meera Nanjundan Jan 2018

Expression And Function Of Nuclear Receptor Coactivator 4 Isoforms In Transformed Endometriotic And Malignant Ovarian Cells, Stephanie Rockfield, Idhaliz Flores, Meera Nanjundan

Molecular Biosciences Faculty Publications

Iron is proposed to contribute to the transition from endometriosis to specific subtypes of ovarian cancers (OVCAs). Regulation of intracellular iron occurs via a ferritinophagic process involving NCOA4 (Nuclear Receptor Coactivator 4), represented by two major isoforms (NCOA4α and NCOA4β), whose contribution to ovarian cancer biology remains uninvestigated. We thus generated transformed endometriotic cells (via HRASV12A, c-MYCT58A, and p53 inactivation) whose migratory potential was increased in response to conditioned media from senescent endometriotic cells. We identified elevated NCOA4 mRNA in transformed endometriotic cells (relative to non-transformed). Knockdown of NCOA4 increased ferritin heavy chain (FTH1) and p21 …

Go to article

Mbd2-Cp2c Loop Drives Adult-Type Globin Gene Expression And Definitive Erythropoiesis, Min Young Kim, Ji Sook Kim, Seung Han Son, Chang Su Lim, Hea Young Eum, Dae Hyun Ha, Mi Ae Park, Eun Jung Baek, Buom-Yong Ryu, Ho Chul Kang, Vladimir N. Uversky, Chul Geun Kim Jan 2018

Mbd2-Cp2c Loop Drives Adult-Type Globin Gene Expression And Definitive Erythropoiesis, Min Young Kim, Ji Sook Kim, Seung Han Son, Chang Su Lim, Hea Young Eum, Dae Hyun Ha, Mi Ae Park, Eun Jung Baek, Buom-Yong Ryu, Ho Chul Kang, Vladimir N. Uversky, Chul Geun Kim

Molecular Biosciences Faculty Publications

During hematopoiesis, red blood cells originate from the hematopoietic stem cell reservoir. Although the regulation of erythropoiesis and globin expression has been intensively investigated, the underlining mechanisms are not fully understood, including the interplay between transcription factors and epigenetic factors. Here, we uncover that the Mbd2-free NuRD chromatin remodeling complex potentiates erythroid differentiation of proerythroblasts via managing functions of the CP2c complexes. We found that both Mbd2 and Mbd3 expression is downregulated during differentiation of MEL cells in vitro and in normal erythropoiesis in mouse bone marrow, and Mbd2 downregulation is crucial for erythropoiesis. In uninduced MEL cells, the Mbd2-NuRD …

Go to article

Yeast Lifespan Variation Correlates With Cell Growth And Sir2 Expression, Jessica T. Smith, Jill W. White, Huzefa Dungrawala, Hui Hua, Brandt L. Schneider Jan 2018

Yeast Lifespan Variation Correlates With Cell Growth And Sir2 Expression, Jessica T. Smith, Jill W. White, Huzefa Dungrawala, Hui Hua, Brandt L. Schneider

Molecular Biosciences Faculty Publications

Isogenic wild type yeast cells raised in controlled environments display a significant range of lifespan variation. Recent microfluidic studies suggest that differential growth or gene expression patterns may explain some of the heterogeneity of aging assays. Herein, we sought to complement this work by similarly examining a large set of replicative lifespan data from traditional plate assays. In so doing, we reproduced the finding that short-lived cells tend to arrest at senescence with a budded morphology. Further, we found that wild type cells born unusually small did not have an extended lifespan. However, large birth size and/or high inter-generational growth …

Go to article

Radx Modulates Rad51 Activity To Control Replication Fork Protection, Kamakoti P. Bhat, Archana Krishnamoorthy, Huzefa Dungrawala, Edwige B. Garcin, Mauro Modesti, David Cortez Jan 2018

Radx Modulates Rad51 Activity To Control Replication Fork Protection, Kamakoti P. Bhat, Archana Krishnamoorthy, Huzefa Dungrawala, Edwige B. Garcin, Mauro Modesti, David Cortez

Molecular Biosciences Faculty Publications

RAD51 promotes homologous recombination repair (HR) of double-strand breaks and acts during DNA replication to facilitate fork reversal and protect nascent DNA strands from nuclease digestion. Several additional HR proteins regulate fork protection by promoting RAD51 filament formation. Here, we show that RADX modulates stalled fork protection by antagonizing RAD51. Consequently, silencing RADX restores fork protection in cells deficient for BRCA1, BRCA2, FANCA, FANCD2, or BOD1L. Inactivating RADX prevents both MRE11- and DNA2-dependent fork degradation. Furthermore, RADX overexpression causes fork degradation that is dependent on these nucleases and fork reversal. The amount of RAD51 determines the fate of stalled replication …

Go to article

Septal Secretion Of Protein A In Staphylococcus Aureus Requires Seca And Lipoteichoic Acid Synthesis, Wenqi Yu, Dominique Missiakas, Olaf Schneewind Jan 2018

Septal Secretion Of Protein A In Staphylococcus Aureus Requires Seca And Lipoteichoic Acid Synthesis, Wenqi Yu, Dominique Missiakas, Olaf Schneewind

Molecular Biosciences Faculty Publications

Surface proteins of Staphylococcus aureus are secreted across septal membranes for assembly into the bacterial cross-wall. This localized secretion requires the YSIRK/GXXS motif signal peptide, however the mechanisms supporting precursor trafficking are not known. We show here that the signal peptide of staphylococcal protein A (SpA) is cleaved at the YSIRK/GXXS motif. A SpA signal peptide mutant defective for YSIRK/GXXS cleavage is also impaired for septal secretion and co-purifies with SecA, SecDF and LtaS. SecA depletion blocks precursor targeting to septal membranes, whereas deletion of secDF diminishes SpA secretion into the cross-wall. Depletion of LtaS blocks lipoteichoic acid synthesis and …

Go to article

First Prev Next Last