Life Sciences Commons^™

Interrogation Of Protein Function With Peptidomimetics, Olapeju Bolarinwa

USF Tampa Graduate Theses and Dissertations

Proteins can be described as the “machineries” responsible for almost all tasks in the levels of organizational complexity in multi-cellular organisms namely: the cells, tissues, organs and systems. Any disorder in the function of a protein at any of these levels could result in disease, and a study of protein function is critical to understanding the pathological features of the disease at the molecular level. A quick glance at these abundantly present proteins reveals two striking features: large diversity of biological function, and the variations in structural complexity, which varies from simple random coils, to turns and helices, and on …

Phytoplankton Size Class In The East China Sea Derived From Modis Satellite Data, Hailong Zhang, Shengquiang Wang, Zhongfeng Qiu, Deyong Sun, Joji Ishizaka, Shaojie Sun, Yijun He

Marine Science Faculty Publications

The distribution and variation of phytoplankton size class (PSC) are key to understanding ocean biogeochemical processes and ecosystems. Remote sensing of the PSC in the East China Sea (ECS) remains a challenge, although many algorithms have been developed to estimate PSC. Here based on a local dataset from the ECS, a regional model was tuned to estimate the PSC from the spectral features of normalized phytoplankton absorption (aph) using a principal component analysis approach. Before applying the refined PSC model to MODIS (Moderate Resolution Imaging Spectroradiometer) data, reconstructing satellite remote sensing reflectance (Rrs) at 412 and …

Simulating Transport Pathways Of Pelagic Sargassum From The Equatorial Atlantic Into The Caribbean Sea, Nathan F. Putman, Gustavo J. Goni, Lewis J. Gramer, Chuanmin Hu, Elizabeth M. Johns, Joaquin Trinanes, Mengqiu Wang

Marine Science Faculty Publications

Since 2011, beach inundation of massive amounts of pelagic Sargassum algae has occurred around the Caribbean nations and islands. Previous studies have applied satellite ocean color to determine the origins of this phenomenon. These techniques, combined with complementary approaches, suggest that, rather than blooms originating in the Caribbean, they arrive from the Equatorial Atlantic. However, oceanographic context for these occurrences remains limited. Here, we present results from synthetic particle tracking experiments that characterize the interannual and seasonal dynamics of ocean currents and winds likely to influence the transport of Sargassum from the Equatorial Atlantic into the Caribbean Sea. Our findings …

Diurnal Changes Of Cyanobacteria Blooms In Taihu Lake As Derived From Goci Observations, Lin Qi, Chuanmin Hu, Petra M. Visser, Ronghua Ma

Marine Science Faculty Publications

Using frequent and long‐term measurements (eight times per day, 2011 to present) from a geostationary satellite sensor (Geostationary Ocean Color Imager, GOCI), this study investigates diurnal changes of cyanobacterium Microcystis aeruginosa blooms (near‐surface high concentrations or surface scums) in Taihu Lake, from which vertical migration patterns could be inferred. After proper atmospheric correction, a cyanobacterial index algorithm is used to quantify equivalent surface cyanobacterial density (σ, 0–100%) at both pixel and synoptic scales from each cloud‐free image, followed by analysis of diurnal changing patterns of σ at both scales. Three typical diurnal changing patterns are identified from all …

Biotime: A Database Of Biodiversity Time Series For The Anthropocene, Maria Dornelas, Laura H. Antão, Faye Moyes, Amanda E. Bates, Anne E. Magurran, Dušan Adam, Asem A. Akhmetzhanova, Ward Appeltans, José M. Arcos, Christopher D. Stallings

Marine Science Faculty Publications

Motivation: The BioTIME database contains raw data on species identities and abundances in ecological assemblages through time. These data enable users to calculate temporal trends in biodiversity within and amongst assemblages using a broad range of metrics. BioTIME is being developed as a community‐led open‐source database of biodiversity time series. Our goal is to accelerate and facilitate quantitative analysis of temporal patterns of biodiversity in the Anthropocene.

Main types of variables included: The database contains 8,777,413 species abundance records, from assemblages consistently sampled for a minimum of 2 years, which need not necessarily be consecutive. In addition, the database contains …

Anemarrhena Asphodeloides Bunge And Its Constituent Timosaponin‐Aiii Induce Cell Cycle Arrest And Apoptosis In Pancreatic Cancer Cells, Catherine B. Marelia, Arielle Sharp, Tiffany A. Shemwell, Y. C. Zhang, Brant R. Burkhardt

Molecular Biosciences Faculty Publications

Pancreatic cancer is one of the most recalcitrant and lethal of all cancers. We examined the effects of Anemarrhena asphodeloides (AA) and timosaponin‐AIII (TAIII), a steroidal saponin present in AA, on pancreatic cancer cell proliferation and aimed to elucidate their potential apoptotic mechanisms of action. Viability assays and cell cycle analysis revealed that both AA and TAIII significantly inhibited pancreatic cancer cell proliferation and cell cycle progression compared to treatment with gemcitabine, the standard chemotherapeutic agent for advanced pancreatic cancer. We identified a dose‐dependent increase in caspase‐dependent apoptosis and activation of pro‐apoptotic PI3K/Akt pathway proteins, with a subsequent downregulation of …

The Influence Of Landscape And Environmental Factors On Ranavirus Epidemiology In A California Amphibian Assemblage, Brian J. Tornabene, Andrew R. Blausten, Cheryl J. Briggs, Dana M. Calhoun, Pieter T. J. Johnson, Travis Mcdevitt-Galles, Jason R. Rohr, Jason T. Hoverman

Integrative Biology Faculty and Staff Publications

1. A fundamental goal of disease ecology is to determine the landscape and environmental processes that drive disease dynamics at different biological levels to guide management and conservation. Although ranaviruses (family Iridoviridae) are emerging amphibian pathogens, few studies have conducted comprehensive field surveys to assess potential drivers of ranavirus disease dynamics.
2. We examined the factors underlying patterns in site‐level ranavirus presence and individual‐level ranavirus infection in 76 ponds and 1,088 individuals representing five amphibian species within the East Bay region of California.
3. Based on a competing‐model approach followed by variance partitioning, landscape and biotic variables explained the most variation in …

The Role Of Pulmonary Stretch Receptor Afferents In Swallow-Breathing Coordination: A Comparison Of Central Respiratory Rhythm Versus Mechanical Ventilation On Swallow In A Decerebrate Feline Model, Kofi-Kermit A. Horton

USF Tampa Graduate Theses and Dissertations

Swallowing is an essential motor act that coordinates the movement of food or saliva from the mouth through the pharynx and into the esophagus while protecting the upper airways from aspiration of those materials. Disordered swallowing, or dysphagia, results when bolus movement from the oropharyngeal phase into the esophageal phase is uncoordinated. Dysphagia directly causes or increases the risk of aspiration during swallowing in many clinical pathologies including Parkinson’s disease, Alzheimer’s disease, cerebrovascular incidents (stroke) in addition to being prevalent among the elderly population. The coordination between breathing and swallowing is mediated through the interaction of the swallow and respiratory …

A New Approach To The Development Of An Rsv Anti-Viral Targeted Nanocarrier For Dual Inhibition Of Viral Infection And Replication, Anthony N. Singer

USF Tampa Graduate Theses and Dissertations

Respiratory Syncytial Virus (RSV) is a potentially life-threatening respiratory pathogen that infects approximately 64 million children and immunocompromised adults globally per year. Currently, there is a need for prophylactic and therapeutic approaches effective against primary and secondary RSV infections. This project focuses on the development of a simple, smart, and scalable anti-RSV nanotherapeutic that combines novel cellular antiviral defense mechanisms targeting the inhibition of viral fusion and replication. An ICAM-1 targeted liposomal nanocarrier will be synthesized and coated with a layer of chitosan containing the anti-fusion HR2-D peptide as an extracellular defense mechanism. Additionally, chitosan complexed to dual expressing short …

The Nutrition Transition Among The Andean Kichwas Of Ecuador, Vanessa Alicia Chee

USF Tampa Graduate Theses and Dissertations

The nutrition transition is a global phenomenon in which diets have become increasingly westernized and processed while lifestyles have shifted from labor intensive to sedentary, largely on account of the advent of technology, globalization n and urbanization. Despite the prevalence of this phenomenon, very little is known regarding how the nutrition transition has affected the risk of comorbid chronic diseases among indigenous Kichwas communities in Ecuador. Aims of this study are : 1) Identify specific health outcomes (such as diabetes, hypertension, and high cholesterol) associated with the lifestyle risk factors of the nutrition transition among the Andean Kichwas indigenous population; …

Intraspecific Variation In The Recruitment Dynamics Of A Transgressing Avicennia Germinans Population, Shannon Victoria Grogan

USF Tampa Graduate Theses and Dissertations

Survival and establishment of mangrove propagules at higher tidal elevations beyond the landward margin of their distribution is a requirement for the continued existence of mangrove populations in response to rising sea-level. Despite the growing body of literature that discusses mangrove recruitment patterns, few studies have empirically examined establishment and post-establishment growth success of propagules at the higher intertidal positions into witch mangrove populations are migrating. Using an experimental field approach, this study compares establishment and post-establishment growth success of propagules at three positions across a tidal elevation gradient within a landward-transgressing mangrove population of SW Florida (USA). I observed …

Ecophysiology Of Oxygen Supply In Cephalopods, Matthew A. Birk

USF Tampa Graduate Theses and Dissertations

Cephalopods are an important component of many marine ecosystems and support large fisheries. Their active lifestyles and complex behaviors are thought to be driven in large part by competition with fishes. Although cephalopods appear to compete successfully with fishes, a number of their important physiological traits are arguably inferior, such as an inefficient mode of locomotion via jet propulsion and a phylogenetically limited means of blood-borne gas transport. In active shallow-water cephalopods, these traits result in an interesting combination of very high oxygen demand and limited oxygen supply. The ability to maintain active lifestyles despite these metabolic constraints makes cephalopods …

Design And Testing Of A Passive Prosthetic Ankle Foot Optimized To Mimic An Able-Bodied Gait, Millicent Schlafly

USF Tampa Graduate Theses and Dissertations

Currently there are nearly 2 million people living with limb loss in the United States [1]. Many of these individuals are either transtibial (below knee) or transfemoral (above knee) amputees and require an ankle-foot prosthesis for basic mobility. While there are an abundance of options available for individuals who require an ankle-foot prosthesis, these options fail to mimic an intact ankle when it comes to key evaluation criteria such as range of motion, push-off force, and roll over shape. The roll over shape is created by plotting the center of pressure during a step in a shank-based coordinate system. To …

Quantitative Proteomics To Support Translational Cancer Research, Melissa Hoffman

USF Tampa Graduate Theses and Dissertations

Altered signaling pathways, which are mediated by post-translational modifications and changes in protein expression levels, are key regulators of cancer initiation, progression, and therapeutic escape. Many aspects of cancer progression, including early carcinogenesis and immediate response to drug treatment, are beyond the scope of genetic profiling and non-invasive monitoring techniques. Global protein profiling of cancer cell line models, tumor tissues, and biofluids (e.g. serum or urine) using mass spectrometry-based proteomics produces novel biological insights, which support improved patient outcomes. Recent technological advances resulting in next-generation mass spectrometry instrumentation and improved bioinformatics workflows have led to unprecedented measurement reproducibility as well …

Bacterial Diversity Associated With Mineral Substrates And Hot Springs From Caves And Tunnels Of The Naica Underground System (Chihuahua, Mexico), Adriana Espino Del Castillo, Hugo Beraldi-Campesi, Patricia Amador-Lemus, Hiram Isaac Beltrán, Sylvie Le Borgne

International Journal of Speleology

The Naica Underground System (NUS) in Northern Mexico comprises a lead, zinc, and silver producing mine and displays the largest gypsum crystals ever found in natural caves. The caves are now closed to the public and mining activities have been suspended for an undefined period since October 2015. Besides its geological, economical, and tourist importance, the bacterial diversity in the NUS has not been fully explored yet. This study surveyed for bacteria present on different mineral substrates (gypsum crystals, iron oxide crusts) and hot spring samples collected before the NUS was inaccessible, using culture-dependent and culture–independent (PCR-DGGE) methods. This study …

Synthesis, Oxidation, And Distribution Of Polyphenols In Strawberry Fruit During Cold Storage, Katrina E. Kelly

USF Tampa Graduate Theses and Dissertations

Plants inherently produce polyphenols (i.e., antioxidants) as a response to reduce oxidative stress caused by abusive environmental pre- and postharvest conditions. These antioxidants, as well as vitamin C, are present in considerable levels in strawberries; however, excessive oxidative stress brought on by improper postharvest handling conditions can reduce the levels of antioxidants in the fruit and shorten the shelf-life of strawberries. Nevertheless, it may be possible to utilize strawberry’s naturally occurring polyphenols to reduce postharvest stress and extend their shelf life. The polyphenolic profile has been previously investigated in several strawberry cultivars, however no studies have determined the unique polyphenolic …

Understanding Agricultural-Land Conservation From The Perspective Of Landowners In Franklin County, Massachusetts, Rocio Lalanda

USF Tampa Graduate Theses and Dissertations

What motivates agricultural-land owners to use conservation easements? As these legal tools have become a popular strategy for private land conservation in the U.S., a growing body of literature is examining how and why landowners conserve their properties through conservation easements. This research project expands upon environmental, geographical and rural land development literature through a qualitative fieldwork study of 34 private, conservation landowners associated with the Franklin Land Trust, a nonprofit conservation organization in western Massachusetts. The study identifies a broad range of environmental, social, spiritual and financial motivations for agricultural-land owners to conserve their properties, and indicates that for …

Antimicrobial Activities Of Culturable Microorganisms (Actinomycetes And Fungi) Isolated From Chaabe Cave, Algeria, Larbi Belyagoubi, Nabila Belyagoubi-Benhammou, Valme Jurado, Joëlle Dupont, Sandrine Lacoste, Fatima Djebbah, Fatima Z. Ounadjela, Souad Benaissa, Salim Habi, Djamel E. Abdelouahid, Cesareo Saiz-Jimenez

International Journal of Speleology

The discovery of new antibiotics and other bioactive microbial metabolites is a priority given the frequency of the emerging multi-drug resistant pathogenic microorganisms. Thus, scientists are searching for new antibiotics in microorganisms isolated from extreme habitats such as caves. In the present study, we aimed to perform the first analysis on culturable microorganisms in the Chaabe Cave (Algeria), and to test the antimicrobial activities of the isolates (Streptomyces spp. and Penicillium spp.). The potential for antimicrobial activity of 47 strains of actinomycetes and 23 strains of fungi were tested on Candida albicans, Staphylococcus aureus, Micrococcus luteus, Listeria monocytogenes, …

Wageningen Aquaria/Microcosm Experiments, Benthic Foraminifera Assemblage And Stable Isotope Measurements, Patrick Schwing, David J. Hollander

C-IMAGE data

Dataset contains benthic foraminifera assemblage and stable isotope measurements. Stable isotope measurements are presented in δ13C and δ18O (‰). Diversity indices include Shannon, Fisher’s Alpha and evenness. Benthic foraminifera abundance is reported as individuals per unit mass (indiv./g).

A Systems Chemical Biology Approach For Dissecting Differential Molecular Mechanisms Of Action Of Clinical Kinase Inhibitors In Lung Cancer, Natalia Junqueira Sumi

USF Tampa Graduate Theses and Dissertations

Lung cancer is the second most common cancer type and is associated with high mortality rates. The survival rate for lung cancer patients has increased slowly in the last decade mainly as the result of the development of novel targeted and immune therapies. However, non-small cell lung cancer patients lacking known or actionable driver mutations and small cell lung cancer patients with recurrent disease are still in urgent need of new therapies. Drug repurposing is an efficient way to identify new therapies since it uses clinically relevant small molecule drugs. Determination of off-targets of small molecules is a novel approach …

Microbial Associations Of Four Species Of Algal Symbiont-Bearing Foraminifera From The Florida Reef Tract, Usa, Makenna May Martin

USF Tampa Graduate Theses and Dissertations

Marine microbiome research is a rapidly expanding field of study, as scientists investigate the functions of microbial associations in eukaryotic organisms. Foraminifera are among the most abundant shelled organisms in the oceans, yet little is known of their associated microbiomes. This study investigated microbes associated with four species of Foraminifera that host three kinds of algal endosymbionts. The Order Miliolida, Family Soritidae, was represented by three species: Archaias angulatus and Cyclorbiculina compressa, which both host chlorophyte symbionts, and Sorites orbiculus, which hosts dinoflagellate symbionts. The fourth species, Amphistegina gibbosa, belongs to the Order Rotaliida and hosts diatom endosymbionts. Bacterial DNA …

Gesture: An Online Hand-Drawing Tool For Gene Expression Pattern Search, Chunyan Wang, Yiqing Xu, Xuelin Wang, Li Zhang, Suyun Wei, Qiaolin Ye, Youxiang Zhu, Hengfu Yin, Manoj Nainwal, Luis Tanon-Reyes, Feng Cheng, Tongming Yin, Ning Ye

Molecular Biosciences Faculty Publications

Gene expression profiling data provide useful information for the investigation of biological function and process. However, identifying a specific expression pattern from extensive time series gene expression data is not an easy task. Clustering, a popular method, is often used to classify similar expression genes, however, genes with a ‘desirable’ or ‘user-defined’ pattern cannot be efficiently detected by clustering methods. To address these limitations, we developed an online tool called GEsture. Users can draw, or graph a curve using a mouse instead of inputting abstract parameters of clustering methods. GEsture explores genes showing similar, opposite and time-delay expression patterns with …

Identification Of A Novel Polyamine Scaffold With Potent Efflux Pump Inhibition Activity Toward Multi-Drug Resistant Bacterial Pathogens, Renee Fleeman, Ginamarie Debevec, Kirsten Antonen, Jessie Adams, Radleigh G. Santos, Gregory S. Welmaker, Richard A. Houghten, Marc A. Guilianotti, Lindsey N. Shaw

Molecular Biosciences Faculty Publications

We have previously reported the use of combinatorial chemistry to identify broad-spectrum antibacterial agents. Herein, we extend our analysis of this technology toward the discovery of anti-resistance molecules, focusing on efflux pump inhibitors. Using high-throughput screening against multi-drug resistant Pseudomonas aeruginosa, we identified a polyamine scaffold that demonstrated strong efflux pump inhibition without possessing antibacterial effects. We determined that these molecules were most effective with an amine functionality at R1 and benzene functionalities at R2 and R3. From a library of 188 compounds, we studied the properties of 5 lead agents in detail, observing a fivefold to eightfold decrease …

Accuracy And Precision Of Tidal Wetland Soil Carbon Mapping In The Conterminous United States, James R. Holmquist, Lisamarie Windham-Myers, Norman Bliss, Stephen Crooks, James T. Morris, J. P. Megonigal, Tiffany Troxler, Donald Weller, John Callaway, Joshua L. Breithaupt, Joseph M. Smoak

Marine Science Faculty Publications

Tidal wetlands produce long-term soil organic carbon (C) stocks. Thus for carbon accounting purposes, we need accurate and precise information on the magnitude and spatial distribution of those stocks. We assembled and analyzed an unprecedented soil core dataset, and tested three strategies for mapping carbon stocks: applying the average value from the synthesis to mapped tidal wetlands, applying models fit using empirical data and applied using soil, vegetation and salinity maps, and relying on independently generated soil carbon maps. Soil carbon stocks were far lower on average and varied less spatially and with depth than stocks calculated from available soils …

Comparative Abundance, Species Composition, And Demographics Of Continental Shelf Fish Assemblages Throughout The Gulf Of Mexico, Steven A. Murawski, Ernst B. Peebles, Adolfo Gracia, John W. Tunnell Jr.

Marine Science Faculty Publications

We analyzed the results of the first comprehensive, systematic, fishery‐independent survey of Gulf of Mexico (GoM) continental shelves using data collected from demersal longline sampling off the United States, Mexico, and Cuba. In total, 166 species were sampled from 343 longline sets during 2011–2017, which deployed 153,146 baited hooks, catching 14,938 fish. Abundance, species richness, and Shannon–Wiener diversity indices by station were highest in mid‐shelf depths (~100 m), declining by about half in deeper waters. Six spatial assemblages were identified by testing the results of cluster analysis using similarity profile analysis and then plotting the geographic location of identified station …

Off-Target Based Drug Repurposing Using Systems Pharmacology, Brent M. Kuenzi

USF Tampa Graduate Theses and Dissertations

The goal of this study was to identify novel drug repurposing opportunities in cancer by utilizing the off-target profiles of clinically relevant kinase inhibitors. This was based on the observation that the global target profiles of compounds are largely ignored and that many compounds have activity that cannot be explained by their cognate target alone. Additionally, by utilizing clinically relevant compounds, any results would hold a high potential for eventual clinical implementation. We utilized a systems pharmacology approach utilizing cell viability-based drug screening to identify compounds with beneficial off-target activity and then using chemical and phosphoproteomics in order to elucidate …

The Effects Of Gopher Tortoise (Gopherus Polyphemus) Herbivory On Plant Community Composition And Seed Germination, And The Effects Of Gut Passage On The Germinability Of Seeds: A Meta-Analysis, Jason C. Richardson

USF Tampa Graduate Theses and Dissertations

Herbivory produces direct and indirect effects on plants and at different spatial scales will have varied consequences. Consumption of plants by vertebrate grazers may affect the plants on an organismal level through direct mortality, on a community level by changing species composition or by altering the rate of succession, and even at a whole ecosystem level by altering nutrient cycles.

The majority of the scientific literature has focused extensively on herbivory by mammals and birds. With regard to mammals, studies have shown how folivory affects individual plants, plant populations, and communities of plants.

Mammals, as well as birds, also ingest …

Do All “Good Mothers” Breastfeed? How African American Mothers’ Values And Experiences Of Early Motherhood Influence Their Infant Feeding Choices, Airia S. Papadopoulos

USF Tampa Graduate Theses and Dissertations

The food an infant is fed can reflect many things: a source of nutrition, the social and cultural circumstances into which an infant is born, or even a family’s beliefs about the body and breast milk as a source of nutrition. Exclusive breastfeeding, currently the gold standard for infant feeding in the United States (US), is often identified as an expectation in discourses on being a “good mother.” African American mothers in particular are the least likely group in the US to breastfeed in any capacity and many efforts are underway to increase the breastfeeding rates of this population.

This …

Sulfur Cave (Romania), An Extreme Environment With Microbial Mats In A Co2-H2S/O2 Gas Chemocline Dominated By Mycobacteria, Serban M. Sarbu, Joost W. Aerts, Jean-François Flot, Rob J.M. Van Spanning, Calin Baciu, Artur Ionescu, Boglárka M. Kis, Reka Incze, Sándor Sikó-Barabási, Zoltan Para, Botond Hegyeli, Nicu-Viorel Atudorei, Casey Barr, Kenneth Nealson, Ferenc L. Forray, Cristian Lascu, Emily J. Fleming, Wilbert Bitter, Radu Popa

International Journal of Speleology

Sulfur Cave (Puturosu Mountain, Romania) is an extreme environment, unique for displaying life in a gas chemocline. The lower part of the cave is filled with CO₂, CH₄, and H₂S of mofettic origin, while the upper part contains air that floats above the heavier volcanic gasses. S° and H₂SO₄ (from sulfur-oxidation) cover the cave wall at and below the CO₂-H₂S:O₂ gas/gas interface. On the cave wall, near the interface the pH is < 1 and unusual microbial biofilms occur on the rock’s surface. We provide context information on the geology, mineralogy, chemistry and biology to better understand this unique environment. We have used X-ray diffraction, optical microscopy, scanning electron microscopy with EDAX capabilities, stable isotope analysis and 16S and 18S rDNA amplicon sequencing. The most common taxa in the microbial biofilms are Mycobacteria, Acidithiobacillus and Ferroplasmaceae. Liquid water in this system originates solely from condensation of water …

Beyond Chlorophyll Fluorescence: The Time Is Right To Expand Biological Measurements In Ocean Observing Programs, Emmanuel Boss, Anya Waite, Anya Waite, Frank Muller-Karger, Hidekatsu Yamazaki, Rik Wanninkhof, Julia Uitz, Sandy Thomalla, Heidi Sosik, Bernadette Sloyan, Anthony Richardson, Johannes Karstensen, Gérald Grégori, Katja Fennel, Herve Claustre, Marcela Cornejo, Ilana Berman‐Frank, Sonia Batten, Silvia Acinas

Marine Science Faculty Publications

A new Scientific Committee for Ocean Research (SCOR) working group has been formed, entitled SCOR WG-154 “Integration of Plankton-Observing Sensor Systems to Existing Global Sampling Programs (P-OBS)”. The working group (P-OBS WG) is reviewing biological sensing technologies and measurements that are ready for integration into existing regional and global ocean observing programs. Multidisciplinary sets of measurements, whose choice is guided by research and societal benefit goals, will transform our understanding of ocean biology and its impacts on Earth systems. Together, we hope to facilitate biological sampling of the oceans and promote more robust, systematic, and routine analyses. These data will …