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Articles 751 - 780 of 2279

Full-Text Articles in Life Sciences

Cornus Officinalis Significantly Improves Oxidative Capacity And Promotes The Calcium-Dependent Transcription Factor, Nfatc2, In Human 1.1b4 Pancreatic Cell Line, Arielle Sharp, Alexis Coiner, Brant Burkhardt Jan 2019

Cornus Officinalis Significantly Improves Oxidative Capacity And Promotes The Calcium-Dependent Transcription Factor, Nfatc2, In Human 1.1b4 Pancreatic Cell Line, Arielle Sharp, Alexis Coiner, Brant Burkhardt

Molecular Biosciences Faculty Publications

Type 1 diabetes (T1D) is an autoimmune disease resulting in the destruction of pancreatic β cells (β-cells) and subsequent loss of insulin production. The only treatment for T1D is using exogenous insulin coupled with continual glucose monitoring following significant autoimmune destruction of β-cells. Novel interventional therapies are needed that can preserve and protect existing pancreatic β cells in individuals with early identified T1D autoimmunity. Our initial in-vitro evidence indicates Cornus officinalis (CO) may be able to serve in this function. What sets ethnopharmacology apart from conventional medicine is the simultaneous targeting of multiple mechanisms using a single herb due to …


Structural Basis Of Phosphatidylcholine Recognition By The C2–Domain Of Cytosolic Phospholipase A,2Α, Yoshinori Hirano, Yong-Guang Gao, Daniel J. Stephenson, Ngoc T. Vu, Lucy Malinina, Dhirendra K. Simanshu, Charles E. Chalfant, Dinshaw J. Patel, Rhoderick E. Brown Jan 2019

Structural Basis Of Phosphatidylcholine Recognition By The C2–Domain Of Cytosolic Phospholipase A,2Α, Yoshinori Hirano, Yong-Guang Gao, Daniel J. Stephenson, Ngoc T. Vu, Lucy Malinina, Dhirendra K. Simanshu, Charles E. Chalfant, Dinshaw J. Patel, Rhoderick E. Brown

Molecular Biosciences Faculty Publications

Ca2+-stimulated translocation of cytosolic phospholipase A2α (cPLA2α) to the Golgi induces arachidonic acid production, the rate-limiting step in pro-inflammatory eicosanoid synthesis. Structural insights into the cPLA2α preference for phosphatidylcholine (PC)-enriched membranes have remained elusive. Here, we report the structure of the cPLA2α C2-domain (at 2.2 Å resolution), which contains bound 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) and Ca2+ ions. Two Ca2+ are complexed at previously reported locations in the lipid-free C2-domain. One of these Ca2+ions, along with a third Ca2+, bridges the C2-domain to the DHPC phosphate group, which also interacts with Asn65. Tyr96 plays a key role in lipid …


Conserved Glycines Control Disorder And Function In The Cold-Regulated Protein, Cor15a, Oluwakemi T. Sowemimo, Patrick Knox-Brown, Wade M. Borcherds, Tobias Rindfleisch, Anja Thalhammer, Gary W. Daughdrill Jan 2019

Conserved Glycines Control Disorder And Function In The Cold-Regulated Protein, Cor15a, Oluwakemi T. Sowemimo, Patrick Knox-Brown, Wade M. Borcherds, Tobias Rindfleisch, Anja Thalhammer, Gary W. Daughdrill

Molecular Biosciences Faculty Publications

Cold-regulated (COR) 15A is an intrinsically disordered protein (IDP) from Arabidopsis thaliana important for freezing tolerance. During freezing-induced cellular dehydration, COR15A transitions from a disordered to mostly α-helical structure. We tested whether mutations that increase the helicity of COR15A also increase its protective function. Conserved glycine residues were identified and mutated to alanine. Nuclear magnetic resonance (NMR) spectroscopy was used to identify residue-specific changes in helicity for wildtype (WT) COR15A and the mutants. Circular dichroism (CD) spectroscopy was used to monitor the coil–helix transition in response to increasing concentrations of trifluoroethanol (TFE) and ethylene glycol. The impact of the COR15A …


Multiscale Model Of Regional Population Decline In Little Brown Bats Due To White-Nose Syndrome, Andrew M. Kramer, Claire S. Teitelbaum, Ashton Griffin, John M. Drake Jan 2019

Multiscale Model Of Regional Population Decline In Little Brown Bats Due To White-Nose Syndrome, Andrew M. Kramer, Claire S. Teitelbaum, Ashton Griffin, John M. Drake

Integrative Biology Faculty and Staff Publications

  1. The introduced fungal pathogen Pseudogymnoascus destructans is causing decline of several species of bats in North America, with some even at risk of extinction or extirpation. The severity of the epidemic of white-nose syndrome caused by P. destructans has prompted investigation of the transmission and virulence of infection at multiple scales, but linking these scales is necessary to quantify the mechanisms of transmission and assess population-scale declines.
  2. We built a model connecting within-hibernaculum disease dynamics of little brown bats to regional-scale dispersal, reproduction, and disease spread, including multiple plausible mechanisms of transmission.
  3. We parameterized the model using the approach of …


A Ctenophore (Comb Jelly) Employs Vortex Rebound Dynamics And Outperforms Other Gelatinous Swimmers, Brad J. Gemmell, Sean P. Colin, John H. Costello, Kelly R. Sutherland Jan 2019

A Ctenophore (Comb Jelly) Employs Vortex Rebound Dynamics And Outperforms Other Gelatinous Swimmers, Brad J. Gemmell, Sean P. Colin, John H. Costello, Kelly R. Sutherland

Integrative Biology Faculty and Staff Publications

Gelatinous zooplankton exhibit a wide range of propulsive swimming modes. One of the most energetically efficient is the rowing behaviour exhibited by many species of schyphomedusae, which employ vortex interactions to achieve this result. Ctenophores (comb jellies) typically use a slow swimming, cilia-based mode of propulsion. However, species within the genus Ocyropsis have developed an additional propulsive strategy of rowing the lobes, which are normally used for feeding, in order to rapidly escape from predators. In this study, we used high-speed digital particle image velocimetry to examine the kinematics and fluid dynamics of this rarely studied propulsive mechanism. This mechanism …


4dee—What's Next? Designing Instruction And Assessing Student Learning, Luanna Prevost, Amanda E. Sorensen, Jennifer H. Doherty, Diane Ebert-May, Bob Pohlad Jan 2019

4dee—What's Next? Designing Instruction And Assessing Student Learning, Luanna Prevost, Amanda E. Sorensen, Jennifer H. Doherty, Diane Ebert-May, Bob Pohlad

Integrative Biology Faculty and Staff Publications

No abstract provided.


Multiscale Model Of Regional Population Decline In Little Brown Bats Due To White-Nose Syndrome, Andrew M. Kramer, Claire S. Teitelbaum, Ashton Griffin, John M. Drake Jan 2019

Multiscale Model Of Regional Population Decline In Little Brown Bats Due To White-Nose Syndrome, Andrew M. Kramer, Claire S. Teitelbaum, Ashton Griffin, John M. Drake

Integrative Biology Faculty and Staff Publications

  1. The introduced fungal pathogen Pseudogymnoascus destructans is causing decline of several species of bats in North America, with some even at risk of extinction or extirpation. The severity of the epidemic of white-nose syndrome caused by P. destructans has prompted investigation of the transmission and virulence of infection at multiple scales, but linking these scales is necessary to quantify the mechanisms of transmission and assess population-scale declines.
  2. We built a model connecting within-hibernaculum disease dynamics of little brown bats to regional-scale dispersal, reproduction, and disease spread, including multiple plausible mechanisms of transmission.
  3. We parameterized the model using the approach of …


Microbial Fe(Iii) Reduction As A Potential Iron Source From Holocene Sediments Beneath Larsen Ice Shelf, Jaewoo Jung, Kyu-Cheul Yoo, Brad E. Rosenheim, Tim M. Conway, Jae Il Lee, Ho Il Yoon, Chung Yeon Hwang, Kiho Yang, Christina Subt, Jinwook Kim Jan 2019

Microbial Fe(Iii) Reduction As A Potential Iron Source From Holocene Sediments Beneath Larsen Ice Shelf, Jaewoo Jung, Kyu-Cheul Yoo, Brad E. Rosenheim, Tim M. Conway, Jae Il Lee, Ho Il Yoon, Chung Yeon Hwang, Kiho Yang, Christina Subt, Jinwook Kim

Marine Science Faculty Publications

Recent recession of the Larsen Ice Shelf C has revealed microbial alterations of illite in marine sediments, a process typically thought to occur during low-grade metamorphism. In situ breakdown of illite provides a previously-unobserved pathway for the release of dissolved Fe2+ to porewaters, thus enhancing clay-rich Antarctic sub-ice shelf sediments as an important source of Fe to Fe-limited surface Southern Ocean waters during ice shelf retreat after the Last Glacial Maximum. When sediments are underneath the ice shelf, Fe2+ from microbial reductive dissolution of illite/Fe-oxides may be exported to the water column. However, the initiation of an oxygenated, …


So What? A Brief History Of Robert N. Ginsburg 1925-2017, Eugene A. Shinn Jan 2019

So What? A Brief History Of Robert N. Ginsburg 1925-2017, Eugene A. Shinn

Marine Science Faculty Publications

This article summarizes the life and work of Robert Ginsburg.


Novel Habitat Causes A Shift To Diurnal Activity In A Nocturnal Species, J. Sean Doody, Colin R. Mchenry, David Rhind, Simon Clulow Jan 2019

Novel Habitat Causes A Shift To Diurnal Activity In A Nocturnal Species, J. Sean Doody, Colin R. Mchenry, David Rhind, Simon Clulow

USF St. Petersburg Campus Faculty Publications

Plastic responses may allow individuals to survive and reproduce in novel environments, and can facilitate the establishment of viable populations. But can novel environments reveal plasticity by causing a shift in a behavior as fundamental and conspicuous as daily activity? We studied daily activity times near the invasion front of the cane toad (Rhinella marina), an invasive species that has colonized much of northern Australia. Cane toads in Australia are nocturnal, probably because diurnal activity would subject them to intolerably hot and dry conditions in the tropical savannah during the dry season. Our study can demonstrate, however, that upon reaching …


Carbon Cycling In The North American Coastal Ocean: A Synthesis, Katja Fennel, Simone Alin, Leticia Barbero, Wiley Evans, Timothée Bourgeois, Sarah Cooley, Frank E. Muller-Karger, Lisa Robbins Jan 2019

Carbon Cycling In The North American Coastal Ocean: A Synthesis, Katja Fennel, Simone Alin, Leticia Barbero, Wiley Evans, Timothée Bourgeois, Sarah Cooley, Frank E. Muller-Karger, Lisa Robbins

Marine Science Faculty Publications

A quantification of carbon fluxes in the coastal ocean and across its boundaries with the atmosphere, land, and the open ocean is important for assessing the current state and projecting future trends in ocean carbon uptake and coastal ocean acidification, but this is currently a missing component of global carbon budgeting. This synthesis reviews recent progress in characterizing these carbon fluxes for the North American coastal ocean. Several observing networks and high-resolution regional models are now available. Recent efforts have focused primarily on quantifying the net air–sea exchange of carbon dioxide (CO2). Some studies have estimated other key …


Minimum Information About An Uncultivated Virus Genome (Miuvig), Simon Roux, Evelien Adriaenssens, Bas Dutilh, Eugene Koonin, Andrew Kropinski, Mart Krupovic, Mya Breitbart, Karyna Rosario Jan 2019

Minimum Information About An Uncultivated Virus Genome (Miuvig), Simon Roux, Evelien Adriaenssens, Bas Dutilh, Eugene Koonin, Andrew Kropinski, Mart Krupovic, Mya Breitbart, Karyna Rosario

Marine Science Faculty Publications

We present an extension of the Minimum Information about any (x) Sequence (MIxS) standard for reporting sequences of uncultivated virus genomes. Minimum Information about an Uncultivated Virus Genome (MIUViG) standards were developed within the Genomic Standards Consortium framework and include virus origin, genome quality, genome annotation, taxonomic classification, biogeographic distribution and in silico host prediction. Community-wide adoption of MIUViG standards, which complement the Minimum Information about a Single Amplified Genome (MISAG) and Metagenome-Assembled Genome (MIMAG) standards for uncultivated bacteria and archaea, will improve the reporting of uncultivated virus genomes in public databases. In turn, this should enable more robust comparative …


Pepper Mild Mottle Virus: Agricultural Menace Turned Effective Tool For Microbial Water Quality Monitoring And Assessing (Waste)Water Treatment Technologies, Erin Symonds, Karyna Rosario, Mya Breitbart Jan 2019

Pepper Mild Mottle Virus: Agricultural Menace Turned Effective Tool For Microbial Water Quality Monitoring And Assessing (Waste)Water Treatment Technologies, Erin Symonds, Karyna Rosario, Mya Breitbart

Marine Science Faculty Publications

No abstract provided.


Mississippi River And Campeche Bank (Gulf Of Mexico) Episodes Of Cross-Shelf Export Of Coastal Waters Observed With Satellites, Daniel Otis, Matthieu Le Hénaff, Vassiliki H. Kourafalou, Lucas Mceachron, Frank Muller-Karger Jan 2019

Mississippi River And Campeche Bank (Gulf Of Mexico) Episodes Of Cross-Shelf Export Of Coastal Waters Observed With Satellites, Daniel Otis, Matthieu Le Hénaff, Vassiliki H. Kourafalou, Lucas Mceachron, Frank Muller-Karger

Marine Science Faculty Publications

The cross-shelf advection of coastal waters into the deep Gulf of Mexico is important for the transport of nutrients or potential pollutants. Twenty years of ocean color satellite imagery document such cross-shelf transport events via three export pathways in the Gulf of Mexico: from the Campeche Bank toward the central Gulf, from the Campeche Bank toward the Florida Straits, and from the Mississippi Delta to the Florida Straits. A catalog of these events was created based on the visual examination of 7280 daily satellite images. Water transport from the Campeche Bank to the central Gulf occurred frequently and with no …


Developing High Resolution Baseline Coast Resource Maps Using World View 2 Imagery For A Coastal Village In Fiji, Ashneel Ajay Singh, Anish Maharaj, Michelle Kumar, Priyatma Singh, Sanjay Singh, Frank Muller-Karger, Matthew James Mccarthy, Lionel Joseph, Herve Damlamian, Zulfikar Begg Jan 2019

Developing High Resolution Baseline Coast Resource Maps Using World View 2 Imagery For A Coastal Village In Fiji, Ashneel Ajay Singh, Anish Maharaj, Michelle Kumar, Priyatma Singh, Sanjay Singh, Frank Muller-Karger, Matthew James Mccarthy, Lionel Joseph, Herve Damlamian, Zulfikar Begg

Marine Science Faculty Publications

In Fiji, like most Pacific Island countries, there have been numerous reports of degradation of coastal resources, including adverse changes in abundance and stock distribution of numerous aquatic species associated with the coastal habitat. To develop effective management plans, assessment of existing coastal resources is pertinent. High spatial resolution satellite imagery, combined with geographic information systems allow for efficient and synoptic mapping of coastal resources to provide a baseline for developing effective and improved management plans. The purpose of this study was to develop a baseline habitat map of the intertidal benthic cover in Komave Village, Coral Coast, Sigatoka, Fiji. …


Towards Comprehensive Observing And Modeling Systems For Monitoring And Predicting Regional To Coastal Sea Level, Rui M. Ponte, Mark Carson, Mauro Cirano, Catia M. Domingues, Svetlana Jevrejeva, Marta Marcos, Gary Mitchum, De Wal, Van De Wal, Philip L. Woodworth, Michaël Ablain, Fabrice Ardhuin, Valérie Ballu, Mélanie Becker, Jérôme Benveniste, Florence Birol, Elizabeth Bradshaw, Anny Cazenave, Mey-Frémaux, P. De Mey-Frémaux, Fabien Durand, Tal Ezer, Lee-Lueng Fu, Ichiro Fukumori, Kathy Gordon, Médéric Gravelle, Stephen M. Griffies, Weiqing Han, Angela Hibbert, Chris W. Hughes, Déborah Idier, Villy H. Kourafalou, Christopher M. Little, Andrew Matthews, Angélique Melet, Mark Merrifield, Benoit Meyssignac, Shoshiro Minobe, Thierry Penduff, Nicolas Picot, Christopher Piecuch, Richard D. Ray, Lesley Rickards, Alvaro Santamaría-Gómez, Detlef Stammer, Joanna Staneva, Laurent Testut, Keith Thompson, Philip Thompson, Stefano Vignudelli, Joanne Williams, Simon D. Williams, Guy Wöppelmann, Laure Zanna, Xuebin Zhang Jan 2019

Towards Comprehensive Observing And Modeling Systems For Monitoring And Predicting Regional To Coastal Sea Level, Rui M. Ponte, Mark Carson, Mauro Cirano, Catia M. Domingues, Svetlana Jevrejeva, Marta Marcos, Gary Mitchum, De Wal, Van De Wal, Philip L. Woodworth, Michaël Ablain, Fabrice Ardhuin, Valérie Ballu, Mélanie Becker, Jérôme Benveniste, Florence Birol, Elizabeth Bradshaw, Anny Cazenave, Mey-Frémaux, P. De Mey-Frémaux, Fabien Durand, Tal Ezer, Lee-Lueng Fu, Ichiro Fukumori, Kathy Gordon, Médéric Gravelle, Stephen M. Griffies, Weiqing Han, Angela Hibbert, Chris W. Hughes, Déborah Idier, Villy H. Kourafalou, Christopher M. Little, Andrew Matthews, Angélique Melet, Mark Merrifield, Benoit Meyssignac, Shoshiro Minobe, Thierry Penduff, Nicolas Picot, Christopher Piecuch, Richard D. Ray, Lesley Rickards, Alvaro Santamaría-Gómez, Detlef Stammer, Joanna Staneva, Laurent Testut, Keith Thompson, Philip Thompson, Stefano Vignudelli, Joanne Williams, Simon D. Williams, Guy Wöppelmann, Laure Zanna, Xuebin Zhang

Marine Science Faculty Publications

A major challenge for managing impacts and implementing effective mitigation measures and adaptation strategies for coastal zones affected by future sea level (SL) rise is our limited capacity to predict SL change at the coast on relevant spatial and temporal scales. Predicting coastal SL requires the ability to monitor and simulate a multitude of physical processes affecting SL, from local effects of wind waves and river runoff to remote influences of the large-scale ocean circulation on the coast. Here we assess our current understanding of the causes of coastal SL variability on monthly to multi-decadal timescales, including geodetic, oceanographic and …


Functional Inhibition Of Acid Sphingomyelinase Disrupts Infection By Intracellular Bacterial Pathogens, Chelsea L. Cockburn, Ryan S. Green, Sheela R. Damle, Rebecca K. Martin, Naomi N. Ghahrai, Punsiri M. Colonne, Marissa S. Fullerton, Daniel H. Conrad, Charles E. Chalfant, Daniel E. Voth, Elizabeth A. Rucks, Stacey D. Gilk, Jason A. Carlyon Jan 2019

Functional Inhibition Of Acid Sphingomyelinase Disrupts Infection By Intracellular Bacterial Pathogens, Chelsea L. Cockburn, Ryan S. Green, Sheela R. Damle, Rebecca K. Martin, Naomi N. Ghahrai, Punsiri M. Colonne, Marissa S. Fullerton, Daniel H. Conrad, Charles E. Chalfant, Daniel E. Voth, Elizabeth A. Rucks, Stacey D. Gilk, Jason A. Carlyon

Molecular Biosciences Faculty Publications

Intracellular bacteria that live in host cell–derived vacuoles are significant causes of human disease. Parasitism of low-density lipoprotein (LDL) cholesterol is essential for many vacuole-adapted bacteria. Acid sphingomyelinase (ASM) influences LDL cholesterol egress from the lysosome. Using functional inhibitors of ASM (FIASMAs), we show that ASM activity is key for infection cycles of vacuole-adapted bacteria that target cholesterol trafficking—Anaplasma phagocytophilum, Coxiella burnetii, Chlamydia trachomatis, and Chlamydia pneumoniae. Vacuole maturation, replication, and infectious progeny generation by A. phagocytophilum, which exclusively hijacks LDL cholesterol, are halted and C. burnetii, for which lysosomal cholesterol accumulation is bactericidal, …


Functional Analysis Of The Replication Fork Proteome Identifies Bet Proteins As Pcna Regulators, Sarah R. Wessel, Kareem N. Mohni, Jessica W. Luzwick, Huzefa Dungrawala, David Cortez Jan 2019

Functional Analysis Of The Replication Fork Proteome Identifies Bet Proteins As Pcna Regulators, Sarah R. Wessel, Kareem N. Mohni, Jessica W. Luzwick, Huzefa Dungrawala, David Cortez

Molecular Biosciences Faculty Publications

Identifying proteins that function at replication forks is essential to understanding DNA replication, chromatin assembly, and replication-coupled DNA repair mechanisms. Combining quantitative mass spectrometry in multiple cell types with stringent statistical cutoffs, we generated a high-confidence catalog of 593 proteins that are enriched at replication forks and nascent chromatin. Loss-of-function genetic analyses indicate that 85% yield phenotypes that are consistent with activities in DNA and chromatin replication or already have described functions in these processes. We illustrate the value of this resource by identifying activities of the BET family proteins BRD2, BRD3, and BRD4 in controlling DNA replication. These proteins …


P53 Phosphomimetics Preserve Transient Secondary Structure But Reduce Binding To Mdm2 And Mdmx, Robin Levy, Emily Gregory, Wade Borcherds, Gary W. Daughdrill Jan 2019

P53 Phosphomimetics Preserve Transient Secondary Structure But Reduce Binding To Mdm2 And Mdmx, Robin Levy, Emily Gregory, Wade Borcherds, Gary W. Daughdrill

Molecular Biosciences Faculty Publications

The disordered p53 transactivation domain (p53TAD) contains specific levels of transient helical secondary structure that are necessary for its binding to the negative regulators, mouse double minute 2 (Mdm2) and MdmX. The interactions of p53 with Mdm2 and MdmX are also modulated by posttranslational modifications (PTMs) of p53TAD including phosphorylation at S15, T18 and S20 that inhibits p53-Mdm2 binding. It is unclear whether the levels of transient secondary structure in p53TAD are changed by phosphorylation or other PTMs. We used phosphomimetic mutants to determine if adding a negative charge at positions 15 and 18 has any effect on the transient …


The Caricomp Network Of Caribbean Marine Laboratories (1985–2007): History, Key Findings, And Lessons Learned, Jorge Cortes, Hazel A. Oxenford, Brigitta I. Van Tussenbroek, Eric Jordan-Dahlgren, Aldo Croquer, Carolina Bastidas, John C. Ogden Jan 2019

The Caricomp Network Of Caribbean Marine Laboratories (1985–2007): History, Key Findings, And Lessons Learned, Jorge Cortes, Hazel A. Oxenford, Brigitta I. Van Tussenbroek, Eric Jordan-Dahlgren, Aldo Croquer, Carolina Bastidas, John C. Ogden

Integrative Biology Faculty and Staff Publications

Caribbean Coastal Marine Productivity (CARICOMP) was a basin-wide cooperative, international network of marine laboratories established in 1985. Recognizing major trends of change in coastal ecosystems and the importance of the linkages among them, our goal was to monitor synoptically with standardized methods the physical environment and to document trends in measures of the structure and functioning of coral reefs, seagrasses and mangroves. Between 1985 and 1993, the CARICOMP Steering Committee established a data management center and wrote a methods manual. Marine laboratories joined the program by appointing a Site Director and signing an agreement specifying the cost sharing and responsibilities …


Comparison Of Dna Methylation In Vibrio Vulnificus Cells Grown In Human Serum With Those Grown In Seawater, James W. Conrad, Valerie J. Harwood Jan 2019

Comparison Of Dna Methylation In Vibrio Vulnificus Cells Grown In Human Serum With Those Grown In Seawater, James W. Conrad, Valerie J. Harwood

Integrative Biology Faculty and Staff Publications

The chromosomal methylation statuses of the highly virulent Vibrio vulnificus strain CMCP6 grown in human serum and in seawater are compared here. Growth in seawater resulted in ∼4 times as much methylation as that in human serum, primarily N4-methylcytosines.


Through The Eyes Of Faculty: Using Personas As A Tool For Learner-Centered Professional Development, Patricia Zagallo, Jill Mccourt, Robert Idsardi, Michelle K. Smith, Mark Urban-Lurain, Tessa C. Andrews, Kevin Haudek, Jennifer K. Knight, John Merrill, Ross Nehm, Luanna B. Prevost, Paula P. Lemons Jan 2019

Through The Eyes Of Faculty: Using Personas As A Tool For Learner-Centered Professional Development, Patricia Zagallo, Jill Mccourt, Robert Idsardi, Michelle K. Smith, Mark Urban-Lurain, Tessa C. Andrews, Kevin Haudek, Jennifer K. Knight, John Merrill, Ross Nehm, Luanna B. Prevost, Paula P. Lemons

Integrative Biology Faculty and Staff Publications

College science instructors need continuous professional development (PD) to meet the call to evidence-based practice. New PD efforts need to focus on the nuanced blend of factors that influence instructors’ teaching practices. We used persona methodology to describe the diversity among instructors who were participating in a long-term PD initiative. Persona methodology originates from ethnography. It takes data from product users and compiles those data in the form of fictional characters. Personas facilitate user-centered design. We identified four personas among our participants: Emma the Expert views herself as the subject-matter expert in the classroom and values her hard-earned excellence in …


The Otud5–Ubr5 Complex Regulates Fact-Mediated Transcription At Damaged Chromatin, Angelo De Vivo, University Of South Florida, Jose Yegres, Jeonghyeon Kim, Sylvia Emly, Younghoon Kee Jan 2019

The Otud5–Ubr5 Complex Regulates Fact-Mediated Transcription At Damaged Chromatin, Angelo De Vivo, University Of South Florida, Jose Yegres, Jeonghyeon Kim, Sylvia Emly, Younghoon Kee

Molecular Biosciences Faculty Publications

Timely stalling and resumption of RNA polymerases at damaged chromatin are actively regulated processes. Prior work showed an importance of FACT histone chaperone in such process. Here we provide a new role of OTUD5 deubiquitinase in the FACT-dependent process. Through a DUB RNAi screen, we found OTUD5 as a specific stabilizer of the UBR5 E3 ligase. OTUD5 localizes to DNA double strand breaks (DSBs), interacts with UBR5 and represses the RNA Pol II elongation and RNA synthesis. OTUD5 co-localizes and interacts with the FACT component SPT16 and antagonizes the histone H2A deposition at DSB lesions. OTUD5 interacts with UBR5 and …


The North Atlantic Aerosol And Marine Ecosystem Study (Naames): Science Motive And Mission Overview, Michael J. Behrenfeld, Richard H. Moore, Chris A. Hostetler, Jason Graff, Peter Gaube, Lynn M. Russell, Gao Chen, Scott C. Doney, Stephen Giovannoni, Hongyu Liu, Christopher Proctor, Luis M. Bolaños, Nicholas Baetge, Cleo Davie-Martin, Toby K. Westberry, Timothy S. Bates, Thomas G. Bell, Kay D. Bidle, Emmanuel S. Boss, Sarah D. Brooks, Brian Cairns, Craig Carlson, Kimberly Halsey, Elizabeth L. Harvey, Chuanmin Hu, Lee Karp-Boss, Mary Kleb, Susanne Menden-Deuer, Françoise Morison, Patricia K. Quinn, Amy Jo Scarino, Bruce Anderson, Jacek Chowdhary, Ewan Crosbie, Richard Ferrare, Johnathan W. Hair, Yongxiang Hu, Scott Janz, Jens Redemann, Eric Saltzman, Michael Shook, David A. Siegel, Armin Wisthaler, Melissa Yang Martin, Luke Ziemba Jan 2019

The North Atlantic Aerosol And Marine Ecosystem Study (Naames): Science Motive And Mission Overview, Michael J. Behrenfeld, Richard H. Moore, Chris A. Hostetler, Jason Graff, Peter Gaube, Lynn M. Russell, Gao Chen, Scott C. Doney, Stephen Giovannoni, Hongyu Liu, Christopher Proctor, Luis M. Bolaños, Nicholas Baetge, Cleo Davie-Martin, Toby K. Westberry, Timothy S. Bates, Thomas G. Bell, Kay D. Bidle, Emmanuel S. Boss, Sarah D. Brooks, Brian Cairns, Craig Carlson, Kimberly Halsey, Elizabeth L. Harvey, Chuanmin Hu, Lee Karp-Boss, Mary Kleb, Susanne Menden-Deuer, Françoise Morison, Patricia K. Quinn, Amy Jo Scarino, Bruce Anderson, Jacek Chowdhary, Ewan Crosbie, Richard Ferrare, Johnathan W. Hair, Yongxiang Hu, Scott Janz, Jens Redemann, Eric Saltzman, Michael Shook, David A. Siegel, Armin Wisthaler, Melissa Yang Martin, Luke Ziemba

Marine Science Faculty Publications

The North Atlantic Aerosols and Marine Ecosystems Study (NAAMES) is an interdisciplinary investigation to improve understanding of Earth's ocean ecosystem-aerosol-cloud system. Specific overarching science objectives for NAAMES are to (1) characterize plankton ecosystem properties during primary phases of the annual cycle and their dependence on environmental forcings, (2) determine how these phases interact to recreate each year the conditions for an annual plankton bloom, and (3) resolve how remote marine aerosols and boundary layer clouds are influenced by plankton ecosystems. Four NAAMES field campaigns were conducted in the western subarctic Atlantic between November 2015 and April 2018, with each campaign …


The Effect Of Ocean Warming On Black Sea Bass (Centropristis Striata) Aerobic Scope And Hypoxia Tolerance, Emily Slesinger, Alyssa Andres, Rachael Young, Brad Seibel, Vincent Saba, Beth Phelan, John Rosendale, Daniel Wieczorek, Grace Saba Jan 2019

The Effect Of Ocean Warming On Black Sea Bass (Centropristis Striata) Aerobic Scope And Hypoxia Tolerance, Emily Slesinger, Alyssa Andres, Rachael Young, Brad Seibel, Vincent Saba, Beth Phelan, John Rosendale, Daniel Wieczorek, Grace Saba

Marine Science Faculty Publications

Over the last decade, ocean temperature on the U.S. Northeast Continental Shelf (U.S. NES) has warmed faster than the global average and is associated with observed distribution changes of the northern stock of black sea bass (Centropristis striata). Mechanistic models based on physiological responses to environmental conditions can improve future habitat suitability projections. We measured maximum, standard metabolic rate, and hypoxia tolerance (Scrit) of the northern adult black sea bass stock to assess performance across the known temperature range of the species. Two methods, chase and swim-flume, were employed to obtain maximum metabolic rate to examine …


Prevalence Of A Vertically Transmitted Single-Stranded Dna Virus In Spinybacked Orbweavers (Gasteracantha Cancriformis) From Florida, Usa, Karyna Rosario, Kaitlin A. Mettel, Anthony M. Greco, Mya Breitbart Jan 2019

Prevalence Of A Vertically Transmitted Single-Stranded Dna Virus In Spinybacked Orbweavers (Gasteracantha Cancriformis) From Florida, Usa, Karyna Rosario, Kaitlin A. Mettel, Anthony M. Greco, Mya Breitbart

Marine Science Faculty Publications

Spiders (order Araneae, class Arachnida) are an important group of predatory arthropods in terrestrial ecosystems that have been recently identified as an untapped reservoir of single-stranded (ss)DNA viruses. Specifically, spiders harbour a diversity of ssDNA viruses encoding a replication-associated protein (Rep) within a circular genome. However, little is known about the ecology of novel circular Rep-encoding ssDNA (CRESS DNA) viruses. Here we investigated two CRESS DNA viruses recently identified in spinybacked orbweavers (Gasteracantha cancriformis), namely spinybacked orbweaver circular virus (SpOrbCV) 1 and 2. SpOrbCV-1 was detected in the majority (> 65 %) of spider specimens from all life …


Sources Of Carbon To Suspended Particulate Organic Matter In The Northern Gulf Of Mexico, Kelsey L. Rogers, Samantha H Bosman, Sarah Weber, Cedric Magen, Joseph P. Montoya, Jeffrey Chanton Jan 2019

Sources Of Carbon To Suspended Particulate Organic Matter In The Northern Gulf Of Mexico, Kelsey L. Rogers, Samantha H Bosman, Sarah Weber, Cedric Magen, Joseph P. Montoya, Jeffrey Chanton

C-IMAGE Publications

Suspended particulate organic carbon (POCsusp) in the Gulf of Mexico is unique compared to other seas and oceans. In addition to surface primary production, isotopic analysis indicates that microbial cycling of oil and riverine inputs are primary sources of carbon to POCsusp in the Gulf. To characterize POCsusp from seep sites and non-seep north central Gulf (NCG) sites potentially affected by the Deepwater Horizon (DWH) spill, we analyzed 277 and 123 samples for δ13C and Δ14C signatures, respectively. Depth, partitioned into euphotic ( < 300 m) and deep ( > 300 m), was the main driver of spatial δ …


Dispersant Enhances Hydrocarbon Degradation And Alters The Structure Of Metabolically Active Microbial Communities In Shallow Seawater From The Northeastern Gulf Of Mexico, Xiaoxu Sun, Lena Chu, Elisa Mercando, Isabel Romero, David Hollander, Joel E Kostka Jan 2019

Dispersant Enhances Hydrocarbon Degradation And Alters The Structure Of Metabolically Active Microbial Communities In Shallow Seawater From The Northeastern Gulf Of Mexico, Xiaoxu Sun, Lena Chu, Elisa Mercando, Isabel Romero, David Hollander, Joel E Kostka

C-IMAGE Publications

Dispersant application is a primary emergency oil spill response strategy and yet the efficacy and unintended consequences of this approach in marine ecosystems remain controversial. To address these uncertainties, ex situ incubations were conducted to quantify the impact of dispersant on petroleum hydrocarbon (PHC) biodegradation rates and microbial community structure at as close as realistically possible to approximated in situ conditions [2 ppm v/v oil with or without dispersant, at a dispersant to oil ratio (DOR) of 1:15] in surface seawater. Biodegradation rates were not substantially affected by dispersant application at low mixing conditions, while under completely dispersed conditions, biodegradation …


An Introduction To Glaciated Margins: The Sedimentary And Geophysical Archive, D. P. Le Heron, K. A. Hogan, E. R. Phillips, M. Huuse, M. E. Busfield, A. G. Graham Jan 2019

An Introduction To Glaciated Margins: The Sedimentary And Geophysical Archive, D. P. Le Heron, K. A. Hogan, E. R. Phillips, M. Huuse, M. E. Busfield, A. G. Graham

Marine Science Faculty Publications

A glaciated margin is a continental margin that has been occupied by a large ice mass, such that glacial processes and slope processes conspire to produce a thick sedimentary record. Ice masses take an active role in sculpting, redistributing and reorganizing the sediment that they erode on the continental shelf, and act as a supply route to large fan systems (e.g. trough mouth fans, submarine fans) on the continental slope and continental rise. To many researchers, the term ‘glaciated margin’ is synonymous with modern day areas fringing Antarctica and the Arctic shelf systems, yet the geological record contains ancient examples …


High-Resolution Trophic Models Reveal Structure And Function Of A Northeast Pacific Ecosystem, Szymon Surma, Villy Christensen, Rajeev Kumar, Cameron H. Ainsworth, Tony J. Pitcher Jan 2019

High-Resolution Trophic Models Reveal Structure And Function Of A Northeast Pacific Ecosystem, Szymon Surma, Villy Christensen, Rajeev Kumar, Cameron H. Ainsworth, Tony J. Pitcher

Marine Science Faculty Publications

This paper examines the structure and dynamics of the marine ecosystem surrounding Haida Gwaii (an archipelago in the southeastern Gulf of Alaska). Based on previous research, a set of improved mass-balanced models was constructed in Ecopath with Ecosim (EwE) to represent ecosystem states existing circa 1900, 1950, and currently. These models feature increased taxonomic and ecological resolution relative to their predecessors across trophic levels and size classes from zooplankton to whales. A more detailed representation of Pacific herring (Clupea pallasii), including its age structure, predators, and prey, was introduced to permit modeling of the ecosystem role of herring …