Life Sciences Commons^™

Distribution Of Polybrominated Diphenyl Ethers Among Demographic Categories, Giorvanni Merilis

USF Tampa Graduate Theses and Dissertations

Polybrominated Diphenyl Ethers (PBDEs) are flame retardants widely used within the United States in various products such as plastics, electronics, textiles and furniture. With an increase in production and usage, PBDEs have recently emerged as a contaminant of concern. Due to their chemical structure, PBDEs have the propensity to bioaccumulate in mammals. In fact, elevated PBDE concentrations have been recorded in human breast milk. Due to the potential widespread exposure to PBDEs, this study investigates human blood concentrations of PBDEs generated through the 2003-2004 National Health and Nutrition Examination Survey. Through the use of statistical modeling, a comparison of mean …

Regulation Of Extracellular Signal-Regulated Kinase By Histone Deacetylase 6, Jheng-Yu Wu

USF Tampa Graduate Theses and Dissertations

Extracellular signal-regulated kinases 1/2 (ERK1/2) are important kinases regulating cell proliferation and cell migration, and have been established as therapeutic targets for cancer treatment. Previously, we found that ERK1 phosphorylates histone deacetylase 6 (HDAC6) to regulate its enzymatic activity. However, whether HDAC6 reciprocally modulates ERK1 activity is unknown. Here, we have discovered that ERK1/2 are acetylated proteins and shown that HDAC6 manipulates ERK1’s kinase activity via deacetylation. We demonstrated that both ERK1 and ERK2 interact with HDAC6 physically. We showed that the acetylation level of GST-ERK1/2 increased in a dose- and time-dependent manner upon treatment with a pan-HDAC inhibitor, Trichostatin …

Osteopontin Plays A Pivotal Role In In Increasing Severity Of Respiratory Syncytial Virus Infection, Viviana Sampayo-Escobar

USF Tampa Graduate Theses and Dissertations

The molecular mechanisms underlying susceptibility to severe respiratory syncytial virus (RSV) infection remain poorly understood. Herein, we report on the role of osteopontin (OPN) in regulation of RSV infection in human epithelial cells and how interleukin-1 beta (IL-1β), a cytokine secreted soon after RSV infection, when persistently expressed can induce OPN expression leading to increased viral infection. We first compared OPN expression in two human epithelial cell lines: HEK-293 and HEp-2. In contrast to HEp-2, HEK-293 expresses low levels of pro-caspase-1 resulting in decreased IL-1β expression in response to RSV infection. We found a correlation between low IL-1β levels and …

A Program Evaluation Of A Worksite Wellness Initiative For Weight Loss, Nicholas Martinez

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to conduct a program evaluation of ACME’s worksite weight loss initiative and collect evidence relative to the efficacy of the program. An anonymous online survey was administered to participants of the weight loss initiative. The survey was designed to gather information relative to the research questions, which explored the initiative’s barriers to participation, alignment of initiative with the goals of participants, utilization of initiative resources, overall strengths, weaknesses, and areas of improvement for the weight loss initiative, as well as the respondents’ general profile for the six dimensions of wellness. Reporting of data included …

Beyond Age And Stage: Consequences Of Individual Variation In Demographic Traits, Erin Elizabeth Feichtinger

USF Tampa Graduate Theses and Dissertations

Within natural populations, individuals vary in their propensity to grow, survive and produce offspring. Additionally, fates of individuals are often correlated and non-independent as a result of shared genes, rearing environment or both. Variation in demographic traits can change population dynamics over time. The effects of variation in individual growth rate on population growth rate, net reproductive rate and generation time are examined, along with quantifying the sources of variation in survival of a long-lived cooperatively breeding bird, the Florida Scrub-Jay (Aphelocoma coerulescens). I built a 2 stage population model with two growth phenotypes, fast and slow, and …

The Role Of Elevated Hyaluronan-Mediated Motility Receptor (Rhamm/Hmmr) In Ovarian Cancer, Stephanie T. Buttermore

USF Tampa Graduate Theses and Dissertations

Ovarian cancer (OC) has the highest mortality among gynecological cancers. The high mortality is associated with the lack of an accurate screening tool to detect disease in early stage. As a result the majority of OCs are diagnosed in late stage. Further, the molecular events responsible for malignant transformation in the ovary remain poorly understood. Consequently, delineating key molecular players driving OC could help elucidate potential diagnostic, prognostic and therapeutic targets.

Receptor for hyaluronan-mediated motility (RHAMM) belongs to a group of hyaladherins, which share a common ability to bind to hyaluronan (HA). Intracellularly, RHAMM is involved in microtubule spindle assembly …

Discovering Antibacterial And Anti-Resistance Agents Targeting Multi-Drug Resistant Eskape Pathogens, Renee Fleeman

USF Tampa Graduate Theses and Dissertations

Antibiotic resistance has been a developing problem for mankind in recent decades and multi-drug resistant bacteria are now encountered that are resistant to all treatment options available. In 2014, the World Health Organization announced that this problem is driving us towards a “post-antibiotic era” that will change the face of modern medicine as we know it. If lack of novel antibiotic development and FDA approval continues, by the year 2050, 10 million people will die each year to an antimicrobial resistant bacterial infection. With lack of pharmaceutical industry involvement in developing novel antibiotics, the responsibility now lies within the academic …

Structure And Activity Of Metallo-Peptides, Christian C. Tang

USF Tampa Graduate Theses and Dissertations

Metal ions are ubiquitously found in all living systems and play vital roles in supporting life forms by performing an array of biological activities. Such biological activities include binding and transforming organic molecules, and also acting as active centers and cofactors for catalysis of various acid-base and redox reactions in biological system. The main focus in bioinorganic chemistry is to elucidate the structural and functional roles of metals in biological systems. Among all transition metal ions, Cu²⁺ and Fe³⁺ are especially versatile and important due to their abilities to go through redox efficiently.

This dissertation can be divided …

Reef Calcifiers Are Adapted To Episodic Heat Stress But Vulnerable To Sustained Warming, Marleen Stuhr, Claire E. Reymond, Vera Rieder, Pamela Hallock, Jörg Rahnenführer, Hildegard Westphal, Michal Kucera

Marine Science Faculty Publications

Shallow marine ecosystems naturally experience fluctuating physicochemical conditions across spatial and temporal scales. Widespread coral-bleaching events, induced by prolonged heat stress, highlight the importance of how the duration and frequency of thermal stress influence the adaptive physiology of photosymbiotic calcifiers. Large benthic foraminifera harboring algal endosymbionts are major tropical carbonate producers and bioindicators of ecosystem health. Like corals, they are sensitive to thermal stress and bleach at temperatures temporarily occurring in their natural habitat and projected to happen more frequently. However, their thermal tolerance has been studied so far only by chronic exposure, so how they respond under more realistic …

Spatial And Temporal Variation In Mangrove Distribution (1950-2014) In Tampa, Florida Usa, Carolyn Cheatham Rhodes

USF Tampa Graduate Theses and Dissertations

I carried out an observational study of historic high resolution aerial imagery spanning six decades (1950-2014) to identify recent and historic spatial extent of mangrove forests, within the municipal boundaries of the City of Tampa, Florida USA. My objectives were to map mangrove distribution and spatial extent and any change or patterns of change discernable. I observed variable patterns of change and rates of expansion varied between sites spatially as well as within sites between time intervals. I found notable changes in mangrove extent in the Tampa from historic and modern aerial imagery for the ~64-year period between 1950 and …

Pollen Forecasting In Sarasota, Florida, Daniel J. Gessman

USF Tampa Graduate Theses and Dissertations

Current predictions of pollen levels rely strictly on historical Averages, regardless of environmental factors that might affect the timing of pollen release by different plants. For this thesis, the goal was to develop a statistical model that will accurately forecast pollen levels by correlating those daily counts to atmospheric and meteorological conditions. This project used ARIMA modeling on IBM’s SPSS Statistics 24 of daily pollen count information for multiple allergenic pollens in the Sarasota County, Florida area over a 11-year period. The pollen species in question for this project are oak and cypress trees, grass, and ragweed pollens; and Alternaria …

The Effect Of Antigen Polymorphisms On Serological Antibody Detection Assays Based Upon The, Kristi M. Miley

USF Tampa Graduate Theses and Dissertations

Onchocerca volvulus is a filarial parasite transmitted to humans by female Simulium spp. black flies. Infection with this parasite can cause blindness and severe skin disease among humans in Africa and the Americas. Enzyme-linked Immunosorbent Assay serological testing of OV-16 antigen is a diagnostic tool for determining effective elimination of the parasite. Programs typically rely on OV–16 ELISA to evaluate the progress towards interruption and/or elimination of disease by mass drug distribution of ivermectin and vector larvicidal control efforts. As elimination grows closer, monoclonal antibody positive controls for OV-16 ELISA become important to develop for Onchocerca testing due to the …

Effects Of An Early Life Immune Challenge On Body Growth, Personality, Mating Behaviors, And Brain Development Of Zebra Finches (Taeniopygia Guttata), Ahmet Kerim Uysal

USF Tampa Graduate Theses and Dissertations

The developmental stress hypothesis predicts that an aversive condition, such as decreased food intake, predation, and social isolation, in the early developmental stage could have long term effects on behaviors and brain development of an animal. In nature, bird nestlings are susceptible to various factors, such as malnutrition, infections, and parasites. Effects of early life stress on adulthood have been extensively studied with some stressors including malnutrition. However, immune challenges as an early life stressor and their long-term programming effects on adult behaviors are yet to be studied in detail. The goal of the current study was to investigate changes …

Time Series Of Northern Gulf Of Mexico Hydrocarbon Data, 2010-2013, Isabel C. Romero

C-IMAGE data

This dataset consist of hydrocarbon measurements in sediment cores collected during the cruises to northern Gulf of Mexico in the R/V Weatherbird II from 2010-2013.

Epigenetic Response To Challenging Environmental Conditions, Marta Robertson

USF Tampa Graduate Theses and Dissertations

The discovery of epigenetic mechanisms has ignited speculation into their role in ecological and evolutionary processes. In particular, the contribution of epigenetic variation to adaptation or phenotypic plasticity that is distinct from genetic variation would be an important addition to existing evolutionary mechanisms. Although the research of epigenetic mechanisms from an ecological and evolutionary (or eco-evolutionary) perspective has been growing, it is still unclear how epigenetic variation might function in natural populations and settings and to what extent it might serve to mediate population response to changing environmental conditions over time. Over the course of my dissertation, I explored the …

The Interaction Of Scale And Temperature In Elastically Powered Movements, Jeffrey P. Olberding

USF Tampa Graduate Theses and Dissertations

For many animals, rapid movements place high power demands on underlying muscles. Storage of muscle energy in elastic structures and the subsequent rapid release of that energy can effectively amplify muscle power. Elastic recoil can also confer thermal robustness to performance in behaviors occurring at variable temperatures. Muscle contractile performance tends to decrease at lower temperatures, but elastic recoil is less affected by temperature. Here I examine the impacts of temperature and scale in systems using elastic recoil and I explore possible interactive effects on movement performance.

I explored the role that muscle contractile properties play in the differences in …

Functional Trade-Offs In Feeding Performance In Salamanders Of The Family Salamandridae, Charlotte M. Stinson

USF Tampa Graduate Theses and Dissertations

Performance is an organism’s ability to accomplish a particular task or behavior, and morphology can have a major impact on the performance of an organism. Salamanders are ecologically diverse and can feed using a variety of behaviors depending on the environment in which feeding occurs. Feeding is accomplished through the use of the hyobranchial apparatus, which lies along the oropharynx, and this structure can have competing roles; in aquatic environments the apparatus is used for suction feeding and works to depress the floor of the mouth, but during terrestrial feeding this structure projects the tongue forward out of the mouth. …

Remote Sensing Estimation Of Surface Oil Volume During The 2010 Deepwater Horizon Oil Blowout In The Gulf Of Mexico: Scaling Up Aviris Observation With Modis Measurements, Chuanmin Hu, Lian Feng

C-IMAGE data

This dataset contains Rayleigh corrected reflectance data from 19 MODIS images collected between April and July 2010, along with their corresponding maps of surface oil volume, maps of relative oil thickness of different classes, and maps of probability distributions of different thicknesses. Surface oil was estimated by spatially scaling up AVIRIS observations to synoptic MODIS measurements, which were the used to derived oil classification and probability maps.

Sargassum Detection Using Modis, Aviris, Landsat, And Hico Imagery, Chuanmin Hu, Lian Feng

C-IMAGE data

This dataset includes Moderate Resolution Imaging Spectroradiometer (MODIS), Airborne Visible-InfraRed Imaging Spectrometer (AVIRIS), Landsat and Hyperspectral Imager for the Coastal Ocean (HICO) images used in the paper: Hu, C., Feng, L., Hardy, R. F., & Hochberg, E. J. (2015). Spectral and spatial requirements of remote measurements of pelagic Sargassum macroalgae. Remote Sensing of Environment, 167, 229–246. doi:10.1016/j.rse.2015.05.022, together with the delineated sargassum features. The in situ spectral measurements used as endmembers to simulate the results are also included.

The Development Of A Platform Interface With The Use Of Virtual Reality To Enhance Upper-Extremity Prosthetic Training And Rehabilitation, Ashley D. Knight

USF Tampa Graduate Theses and Dissertations

This dissertation focuses on the investigation and development of an effective prosthetic training and rehabilitation platform with the use of virtual reality to facilitate an effective process to return amputees to the highest level of independence and functioning possible.

It has been reported that approximately 10 million people live with a limb loss worldwide, with around 30% being an upper-extremity amputee. The sudden loss of a hand or arm causes the loss of fine, coordinated movements, reduced joint range of motion (ROM), proprioceptive feedback and aesthetic appearance, all which can be improved with the use of a prosthesis and proper …

Functional Roles Of Matrix Metalloproteinases In Bone Metastatic Prostate Cancer, Jeremy S. Frieling

USF Tampa Graduate Theses and Dissertations

Skeletal metastasis is a lethal component of many advanced cancers including prostate, the second most common cancer among men. Patients whose prostate cancer is localized and detected early benefit from multiple treatment options ranging from active surveillance to radiation and surgery, resulting in a 5-year survival rate of nearly 100%. Unfortunately, the prognosis and survival for patients with advanced metastatic disease is much worse due to the highly aggressive nature of the disease and a paucity of treatment options. Understanding the mechanisms and interactions that occur between metastatic cancer cells and the bone will enable the future treatment landscape for …

Mitigating The Conflict Between Pitfall-Trap Sampling And Conservation Of Terrestrial Subterranean Communities In Caves, Peter Kozel, Tanja Pipan, Nina Šajna, Slavko Polak, Tone Novak

International Journal of Speleology

Subterranean habitats are known for their rich endemic fauna and high vulnerability to disturbance. Many methods and techniques are used to sample the biodiversity of terrestrial invertebrate fauna in caves, among which pitfall trapping remains one of the most frequently used and effective ones. However, this method has turned out to be harmful to subterranean communities if applied inappropriately. Traditionally, pitfall traps have been placed in caves solely on the ground. Here we present an optimized technique of pitfall trapping to achieve a balance between sampling completeness and minimal disturbance of the fauna in the cave. Monthly we placed traps …

Disease Introduction By Aboriginal Humans In North America And The Pleistocene Extinction, Zachary D. Nickell, Matthew D. Moran

Journal of Ecological Anthropology

While overhunting and climate change have been the major hypotheses to explain the late-Pleistocene New World megafaunal extinctions, the role of introduced disease has only received brief attention. Here, we review pre-Columbian diseases endemic to aboriginal Americans and evaluate their potential to cause large-scale mortality in Pleistocene mammals. Of the probable communicable diseases present in pre-Columbian times, we regard anthrax and tuberculosis as viable candidates. These two diseases demonstrate characteristics that could have made them deadly to immunologically naïve populations. Introduced disease, as a primary cause or interacting with overhunting and climate change, could have contributed to the decline and …

Non-Classical Regulators In Staphylococcus Aureus, Andy Weiss

USF Tampa Graduate Theses and Dissertations

Staphylococcus aureus is a highly problematic human pathogen due to its ability to cause devastating infections, paired with a capacity to withstand the action of a large fraction of available antibiotics. Both pathogenicity and antibiotic resistance are encoded by numerous genomic elements, though the expression of these factors is energetically costly and not always beneficial for cellular survival. Therefore, S. aureus has developed sophisticated regulatory networks to integrate a multitude of signals, enabling it to navigate the delicate balance between its pathogenic lifestyle and baseline needs for cellular energy homeostasis. It is thus imperative to study S. aureus behavior and …

Therapeutic Modulation Of Cancer Metabolism With Dichloroacetate And Metformin, Nathan Patrick Ward

USF Tampa Graduate Theses and Dissertations

The robust glycolytic metabolism of glioblastoma multiforme (GBM) has proven them susceptible to increases in oxidative metabolism induced by the pyruvate mimetic dichloroacetate (DCA). Recent reports demonstrate that the anti-diabetic drug metformin enhances the damaging oxidative stress associated with DCA treatment in cancer cells. We sought to elucidate the role of metformin’s reported activity as a mitochondrial complex I inhibitor in the enhancement of DCA cytotoxicity in the VM-M3 model of GBM. We demonstrated that metformin potentiated DCA-induced superoxide production and that this was required for enhanced cytotoxicity towards VM-M3 cells with the combination. Similarly, rotenone enhanced oxidative stress resultant …

Uncovering Transcriptional Activators And Targets Of Hsf-1 In Caenorhabditis Elegans, Jessica Brunquell

USF Tampa Graduate Theses and Dissertations

In order to survive, cells must be able to cope with a variety of environmental stressors. The heat shock response (HSR) is a pro-survival mechanism employed by cells in response to protein denaturing stress, such as heat. Since its discovery in 1960, the heat shock response has been found to be regulated by the transcription factor heat shock factor 1 (HSF1). During periods of increased stress, HSF1 undergoes a multi-step process of activation that involves homotrimerization, DNA-binding, and post-translational regulatory modifications, all of which ultimately function to control the transcription of chaperone genes. These chaperone genes encode molecular chaperone proteins …

Acetaminophen Associated Neurotoxicity And Its Relevance To Neurodevelopmental Disorders, Seol-Hee Kim

USF Tampa Graduate Theses and Dissertations

Autism is a lifelong neurodevelopmental disorder. The etiology of autism still remains unclear due to the heterogeneous and complex nature of the disorder, however synergistic actions between genetic components and environmental factors have been suggested. Acetaminophen (APAP) is one of the most popular over-the-counter drugs that possess antipyretic and analgesic effects. It is considered a relatively safe and effective within therapeutic doses. Recently, early exposure to APAP has been suggested to be one of the underlying cause of autism. Children are often prescribed APAP to lessen fever or irritability after vaccination during the first year, and APAP may adversely affect …

Experimental Evaluation Of Cooling Effectiveness And Water Conservation In A Poultry House Using Flow Blurring® Atomizers, Rafael M. Rodriguez

USF Tampa Graduate Theses and Dissertations

Increases in population as well as economic improvements in developing countries are generating a larger demand for animal protein products. Current animal growth processes inherently, require the use of water in many forms throughout the growth cycle. Water is the most important natural resource on earth to sustain life, and in many developing countries is a scarce resource that must be used wisely. Studies have revealed that poultry growth can take place with less water consumption, when compared with other sources of animal protein (e.g., cattle, pork). In this research, an evaporative Flow Blurring^® cooling system was considered as …

Acute Kidney Injury And Chronic Kidney Disease, Jin Wei

USF Tampa Graduate Theses and Dissertations

Ischemia and reperfusion are natural steps during kidney transplantation, and IRI is considered one of the most important nonspecific factors affecting allograft dysfunction. Transplanted organs experience several episodes of ischemia, in which cold ischemia occurs during allograft storage in preservation solutions.

Even though cold ischemia has been studied extensively, all of the studies have been carried out in vitro and ex vivo models. There is no in vivo model available to examine renal IRI induced solely by cold ischemia.

In the present study, we developed an in vivo mouse model to study renal IRI induced exclusively by cold ischemia through …

The Optimization Of The Nuclear Protein In Testis (Nut) Antibody And Its Importance And Impact In The Pathology Lab, Lindsey Martinez

USF Tampa Graduate Theses and Dissertations

Optimization in immunohistochemistry is often a time consuming and complex process. There are a varying array of moving parts to consider all while preserving the sensitivity and specificity of the test. When optimizing an antibody it is important to consider the fixation of the tissue and the type of epitope retrieval that would be best suited for the test. The dilution of the primary antibody is a key marker for the efficiency and effectiveness of the laboratory protocol. The purpose of this study was to produce an optimized antibody for the nuclear protein in testis to detect NUT midline carcinoma …