Life Sciences Commons^™

Molecular Mediators Of Axonal Transport And Local Translation Of Cdc42, Rhoa Molecular Mediators Of Axonal Transport And Local Translation Of And Calreticulin Mrnas And Their Implications For Axon Growth And Regeneration, Matthew Davis Zdradzinski

Theses and Dissertations

Neurons are highly polarized cells with expansive cytoplasmic processes, axons and dendrites, which create unique subcellular domains. Localization and translation of mRNAs in these subcellular regions is now recognized as a critical mechanism through which polarized cells establish and maintain the specialized domains that are needed for their functions. RNA profiling studies have uncovered hundreds to thousands of mRNAs in axons and these axonal mRNA profiles differ across neuronal populations, physiological conditions, and developmental growth states. Functionally organizing these mRNA populations into cohorts of coregulated mRNAs via RNA binding proteins allows us to better understand how the transcriptome is regulated …

The Effects Of Variations In Light And Nutrient Availability On Phytoplankton And Zooplankton, Jake Anthony Swanson

Theses and Dissertations

Aquatic ecosystems experience a wide range of variation in resource availability; two of the resources that are most essential to phytoplankton and zooplankton are light and nutrients. Light can vary in both its color and intensity; these variations can directly impact phytoplankton by altering the amount and color of light available for photosynthesis. Nutrient variability, particularly increases in nutrient levels, also alter phytoplankton communities by favoring the growth of certain taxa such as cyanobacteria.

To understand whether phytoplankton respond differently to changes in light availability I investigated whether the diversification of cryptophytes, phytoplankton with diverse light-capturing pigments, showed evidence of …

Modulation Of Eif2Α Signaling And The Integrated Stress Response By Targeted Therapeutic Attenuation Of Pkr Activity Contributes To Protection Of Dyt-Prkra Cells From Apoptosis, Kenneth L. Frederick

Theses and Dissertations

Luteolin is a natural flavonoid present in vegetables, fruits, and medicinal herbs that possesses anti-oxidative, anti-cancer, and anti-inflammatory properties. In this study, we investigated the effect of luteolin on the integrated stress response (ISR), which is an evolutionarily conserved intracellular signaling network essential for adapting to environmental stresses to maintain healthy cells. Dysregulated ISR is involved in the etiology of many human diseases including the movement disorder DYT-PRKRA which is caused by mutations in the PRKRA gene. PRKRA encodes for PACT, the protein activator of interferon-induced, double-stranded RNA (dsRNA)-activated protein kinase PKR.PACT-mediated PKR activation regulates the …

Predictors Of Driving Performance Post-Stroke, Halle Elise Prine

Theses and Dissertations

Driving is a complex skill involving the interaction of various brain functions, making it challenging to determine the independent contributions of each process to driving proficiency. While past studies have identified assessments such as the Useful Field of View Test (UFOV) and the Trial Making Test (TMT) as strong predictors for driving skills, little research has explored the impact of visual search and movement inhibition on driving after stroke. This cross-sectional study includes participants between the ages of 21 to 80 years with residual disability caused by a unilateral middle cerebral artery stroke at least six months prior. The participants …

Effects Of Caffeine On Measures Of Clinical Outcome And Recovery Following Mild Traumatic Brain Injury In Adolescents, Jacob Michael Eade

Theses and Dissertations

OBJECTIVE: The overarching purpose of this study was to investigate the influence of habitual caffeine consumption on mTBI outcomes in adolescents. BACKGROUND: Adolescents who suffer an mTBI have an increased risk for neurological impairment and symptomology. Thus, it is important to elucidate modifiable lifestyle factors that have the potential to influence injury outcomes and recovery. Caffeine is a legal psychoactive compound prevalent among the adolescent population. Marketed as an ergogenic aid caffeine coincides with recreational activities where incidence of mTBI is high. However, no human study has explored the influence of caffeine consumption on mTBI pathology. It was hypothesized that …

The Role Of Environmental Context In Supporting Children’S 24- Hour Movement Behaviors; A Positive Deviance Perspective, Roddrick Dugger

Theses and Dissertations

Background: During summer, children display less favorable movement behaviors than the school year. To investigate the role of environmental context in supporting children’s ability to meet the 24-hour Movement Guidelines (i.e., Moderate-to-Vigorous Physical Activity: ≥ 60 minutes, Sleep duration: 9-11 hours, Screen Time: ≤ 2 hours), this dissertation had three primary aims: 1) Utilize the Positive Deviance Framework to identify unique practices and resources that enabled children to meet guidelines, 2) Examine potential associations between the neighborhood built and social environment with movement behaviors by household poverty experience, and 3) Identify specific features of the family and home environment that …

Diversity, Function, And Phenotypic Plasticity Of Cryptophyte Phycobiliproteins, Kristiaän Merritt

Theses and Dissertations

Cryptophytes are a group of unicellular eukaryotic algae that can be found in a wide range of underwater habitats. Part of their ecological success can be attributed to their diverse array of cryptophyte phycobiliproteins (Cr-PBPs), a pigment class that captures wavelengths of light that are poorly absorbed by chlorophylls. Cryptophytes gained photosynthesis via secondary endosymbiosis in which their ancestor engulfed a red algal endosymbiont. Following endosymbiosis, they deconstructed the red algal photosynthetic machinery to form the Cr-PBP. Since then, the Cr-PBPs have diversified into at least 9 spectrally distinct forms. I investigated the diversity of Cr-PBP light absorption across 76 …

Leveraging Church Environments To Promote Rural Physical Activity And Healthy Eating, Kelsey Rothera Day

Theses and Dissertations

Rural residents experience a greater burden of chronic disease related to physical inactivity and poor diet than their urban counterparts. A promising strategy to improve these health behaviors in rural areas is to leverage existing structures that may support physical activity and healthy eating, such as churches, in public health interventions. Three studies were conducted as part of this dissertation. They were designed to: 1) examine whether the walkability and healthy food density of neighborhoods surrounding rural and urban churches are associated with race, socioeconomic status, and rural/urban status; 2) examine rural church leaders’ views on the role of faith-based …

Effects Of Fucoidan Supplementation On Inflammatory And Immune Response After High-Intensity Exercise, Bridget A. Mcfadden, Caroline S. Vincenty, Akexa J. Chandler, Harry P. Cintineo, Blaine S. Lints, Gianna F. Mastrofini, Shawn M. Arent Ph.D., Cscs*D, Fissn, Facsm, Fnak

Faculty Publications

Introduction: High-intensity exercise (HIE) can damage the musculotendon complex and impact the immune response, resulting in post-exercise inflammation. Sufficient rest and recovery will improve muscular resilience against future damaging bouts; however, HIE with minimal durations of rest is common in athletic competitions that facilitate persistent inflammation and immune dysregulation. Fucoidans are fucose-rich sulfated polysaccharides with demonstrated anti-inflammatory and pro-immune responses. Fucoidans may improve inflammation and immune responses, which may prove beneficial for individuals who regularly engage in repeated HIE. The research purpose was to investigate the safety and efficacy of fucoidans on inflammatory and immune markers following HIE. Methods: Eight …

A Most Surprising Fern: Serendipity And Browsing In Botanical Search, Douglas Tuers

Journal of the South Carolina Academy of Science

This article is a case study of botanical field work in the eastern United States in the early twentieth century. These cases will be analyzed as instances of browsing and serendipity. Browsing and serendipity have a rich literature in information science and this article will draw on this literature in order to better understand serendipity in botany. This article will show how botanical localities support browsing and serendipity for the botanists who search them. This article will also show how botanical institutions and botanists interface with localities in order to further support browsing and serendipity. As a whole this article …

Table Of Contents

Journal of the South Carolina Academy of Science

No abstract provided.

Consolidated Chamber Design And Protocol For Olfactory Conditioning Assay With Drosophila Melanogaster, Sasha Bronovitskiy, Andres Castillo, Michael Yan, Fang Ju Lin

Journal of the South Carolina Academy of Science

The olfactory conditioning assay is widely used in Alzheimer’s disease research to quantify learning and memory in Drosophila melanogaster. The assay tests ability to recall an aversive conditioned stimulus of scent paired with electrical shock when presented a choice between shock-associated and unrelated scents. The T-maze, a commonly used apparatus for olfactory conditioning assays, employs an elevator mechanism to transfer live flies from the shock-delivering training chamber to the scent selection point. This elevator mechanism is known to cause fly casualty. T-mazes are not commercially available and often difficult to reproduce. Other existing variations of olfactory conditioning apparatuses use …

Analysis Of Genes Responsible For Neuronal Morphological Changes In Down Syndrome Hipsc-Derived Neurons, Juhi Patel

Senior Theses

Down syndrome (DS) is a complex neurodevelopmental disorder caused by the trisomy of chromosome 21. DS is the largest genetic cause of intellectual disability, which occurs in varying severity among affected individuals. It is associated with a variety of developmental and cognitive defects, including reduced brain size, impaired synaptic function, and altered neuronal morphology. A number of genes have been identified to play critical roles in the development and maintenance of neuronal morphology, and alterations in the expression of some of these genes are implicated in the morphological changes observed in DS. Knocking out or altering their expression leads to …

Local Translation And Focal Adhesions Are Dysregulated In Down Syndrome, Ashlyn Gotberg, Nikita Kirkise M.S., Kristy Welshhans Ph.D.

Senior Theses

During cellular migration, fibroblasts sense extracellular cues through membrane complexes at their leading edge and can respond to these cues via local translation. Local translation is the process in which mRNAs are transported to subcellular regions and translated in response to specific cues. There is evidence that local translation may be dysregulated in the neurodevelopmental disorder, Down syndrome, which is caused by the triplication of chromosome 21 and results in genome-wide dysregulation of protein expression. To determine if dysregulated local translation contributes to Down syndrome, we used three sets of primary fibroblasts from individuals with Down syndrome and apparently healthy …

Using Podcasts To Bring National Estuarine Research Reserves Into The Classroom For Grades 6-12, Kaitlyn M. Dirr

Senior Theses

In a typical classroom setting, there are significant challenges to exposing students to concepts related to earth sciences and the environment. These challenges are exacerbated when conveying lessons about geographic areas with limited access, such as oceans and coastlines (Louv, 2010). It is now more important than ever for environmental education to improve and adapt to our changing world. Educators may have the opportunity to bring these subjects to life by using media content such as podcasts to introduce students to new places and the scientists, managers and educators that work in those spaces. In the United States, there exists …

Accuracy Of Therapeutic Drug Monitoring In Vancomycin And The Pharmacist Role: A Retrospective Case-Series, Kateryna Parkhomenko

Senior Theses

Vancomycin is a mainstay of therapy for treating virulent and resistant infections, especially methicillin-resistant Staphylococcus aureus (MRSA). However, vancomycin requires therapeutic drug monitoring (TDM) for optimal dosing and treatment. This requires pharmacists to calculate a dosing regimen that correlates to appropriate goal vancomycin concentrations in the blood. Dosing vancomycin can be difficult, as it varies on a patient’s weight, renal function, age, etc. Doses may have to be adjusted in response to out-of-range concentrations, which requires further pharmacy calculations. Inaccurate dosing poses a risk to patient safety and places a resource and time burden on pharmacists. If vancomycin dosing methods …

Mutating Tetur02g09850 Originated From Spider Mites To Enhance Crystallization, Hayley Cash

Senior Theses

This study aimed to explain and adequately utilize several common biochemical laboratory techniques to mutate a portion of the nucleic acid sequence within a specific protein. This protein, referred to as Tetur02g09850 is originated from Tetranychus Urticae, or the Two-Spotted Spider Mite. This mite causes excessive damage to crops and is known to be extremely resistant to most common pesticides. For this reason, understanding the protein structure could be used to explain this acaricide resistance and aid in the development of more effective pesticides. Some of the testing utilized was site-directed mutagenesis, IMAC and gel filtration for protein purification, gel …

Mirna-489 Induces Immunogenic Cell Death In Triple Negative Breast Cancer Cells, Ryan P. Titus

Senior Theses

It has been well established that microRNAs (miRNAs) play an important role in the regulation of gene expression and consequently promoting or downregulating molecular pathways. When dysregulated, miRNAs have been found to serve as important biomarkers for cancer diagnosis and influence tumor initiation and progression. It has been previously established that miRNA-489 is a tumor suppressor microRNA, and it directly targets cell proliferative pathways like the HER2-SHP2-MAPK pathway. In this study, we focus on the role of miRNA-489, in the induction of immunogenic cell death (ICD) in triple-negative breast cancer cell lines. We first examined the effects of miRNA-489 on …

Effect Of Action Selection Demands On The Execution Of Goal-Directed Reaches, James Garrison Riedy

Theses and Dissertations

A well-studied model of cognitive motor function is the process of action selection. It is unclear how the planning demands of a reaching movement interact with the added complexity of s election. Therefore, this thesis investigated the effect of adding selection demands to the performance of skilled reaches in a virtual environment. Thirty young, healthy participants reached ipsilaterally or contralaterally with either their dominant (right hand) or non-dominant (left hand) under two conditions: execute only (EO) and action selection (AS). Selection accuracy was higher for the EO condition than the AS condition for both arms and both directions (left arm: …

Customer Discovery And Applied Research And Development For An Environmental Nanotechnology, Paul Michael Vecchiarelli

Theses and Dissertations

Technology transfer is a process by which an innovative technology, typically produced in a non-commercial setting such as academia, is developed into a potential market opportunity. The first phase of technology transfer, once a technology has been developed, is to identify a customer segment and determine a value proposition that describes what need the technology will fulfill for them. This phase has been systematized into a framework known as customer discovery. Success in customer discovery will indicate technological gaps, such as scale-up, prototyping, and realistic testing, that are needed to deliver the value proposition to the initial customer. Both phases …

Jewish Genetic Diseases: Knowledge Of Reproductive Risk And Cancer Predisposition Among Young Adults Of Ashkenazi Descent, Hayley Kathleen Granger

Theses and Dissertations

Founder mutations within the Ashkenazi Jewish (AJ) population are associated with significantly higher carrier rates for certain severe, life-limiting conditions collectively called Jewish Genetic Diseases (JGDs), and for pathogenic variants in the BRCA1 and BRCA2 (BRCA1/2) cancer susceptibility genes. Efforts to educate AJ individuals about the implications of these founder mutations and available testing have increased the past forty years; however, studies suggest AJ individuals are not well educated on the topic (Hardy et al., 2022; Kaback, 2001; Warsch et al., 2014). Studies evaluating the reason for this gap in knowledge of young AJ adults are lacking. This study aimed …

Assessing The Gaps And Potential Solutions To Improve Access To Genetic Testing For Autistic Individuals, Nisha Dhiren Pandya

Theses and Dissertations

The prevalence of Autism Spectrum Disorder (ASD) continues to increase. Numerous genetic syndromes increase the risk of ASD. Professional organizations have published guidelines on the genetic testing recommended for Autistic individuals. However, healthcare providers do not unanimously offer genetic testing or refer for a genetics evaluation. Notably, parents of Autistic individuals express interest in learning about genetic testing as an option for their children.

This study aimed to identify gaps to improve access to genetic evaluations for Autistic individuals and their families. A survey was sent to healthcare providers across the United States that currently see Autistic individuals. We expected …

Face Validation Of A Spanish Non-Invasive Prenatal Screening Knowledge Scale, Kenya Michelle De Leon

Theses and Dissertations

Non-invasive prenatal screening (NIPS) provides risk assessment for chromosome aneuploidy in pregnancy. Previous studies in the United Kingdom and United States have attempted to measure patient knowledge, decisional conflict, and informed choice in cohorts of English-speaking pregnant patients who elected NIPS (Griffin et al., 2023; Lewis et al., 2016). However, knowledge scales utilized in these studies have not been validated. The current study was conducted in order to assess face validity of a Spanish NIPS knowledge scale that was originally written in English. This scale was developed as part of a larger project to ultimately provide more validated research measures …

Genetic Testing For Autism: The Autistic Adult Perspective, Thomas Scott Dent

Theses and Dissertations

Many professional medical organizations recommend some level of genetic testing as standard of care for those diagnosed with autism spectrum disorder. However, genetic testing related to the diagnosis of autism is seen as controversial by many in the autistic community. While opinions about genetic testing have been well-documented from the perspective of the parents of autistic children, our understanding of the autistic adult perspective remains limited. We implemented a descriptive, web-based survey of autistic adults to assess their awareness of, attitudes towards, and interests in genetic testing for autism (n = 145). Our data demonstrated that half of our participants …

Evaluating Health Awareness In Cancer Genetics Amongst The Black And African American Community In South Carolina, Annika Jaliya Gadson

Theses and Dissertations

Historically minoritized individuals are underrepresented in genomic research which limits the ability to fully understand genetic variation within the population (Sirugo et al., 2019). This creates bias that questions the effectiveness of guidelines for genetic testing, predictive risk values, and medical management. Disparities in cancer genetics may be attributed to historical events that have cultivated mistrust in research and medicine, institutional bias, provider skepticism in patient reporting of medical symptoms, and limited access to genetic testing (Saulsberry et al., 2013). More exploration is needed to better understand how to increase access and awareness of cancer genetic services to Black and …

Genomic Analysis Of Exophiala Sp. Etnp2018, A Marine Fungal Strain Isolated From An Oxygen Minimum Zone, Margaret Kelly Bernish

Theses and Dissertations

Microbial metabolic activity is central to biogeochemical cycling in virtually all ecosystems. Fungi are important parts of microbial communities, serving as decomposers, parasites, and symbionts. Most of our understanding of fungal ecology is derived from the study of isolated, cultured terrestrial fungi. However, the marine environment includes thousands of divergent, uncultured fungi. A number of these species, which include saprobes, parasites, and endophytes, can survive and sporulate in a wide range of salinities. Other such species face the additional challenge of limited nutrient accessibility. The genus Exophiala includes agents of mycoses in both humans and in marine animals. This research …

Exercise And Mental Health Over The Course Of A Semester, Jamie Alexis Whitney

Theses and Dissertations

Introduction and purpose: Anxiety and depression are two of the most common mental health struggles in the United States each year. There has been promising research noting the positive effects of exercise on lowering symptoms of both anxiety and depression. Anxiety and depression are highly prevalent on college campuses, especially during high stress portions of the semester. The purpose of this study was to observe the changes in mental health over the course of the semester and if exercise level mediated any of these changes in the university population. Methods: Participants scanned a QR code or followed a link to …

Genetic Mechanisms Underlying Maladaptation In Specialized Species Interactions, Nitin Ravikanthachari

Theses and Dissertations

Adaptation has been used as the framework to understand the power of natural selection in structuring biodiversity and driving biological interactions. However, recent examples have indicated that many organisms are maladapted to their local conditions. Maladaptation can result from various causes including rapid environmental change, novel environments, interplay of evolutionary forces (viz: gene flow-selection balance) and anthropogenic disturbance.

To dissect the genetic mechanisms underlying maladaptation, I used the interaction between Pieris macdunnoughii (Remington) (Lepidoptera: Pieridae) which lays eggs on an invasive mustard Thlaspi arvense (L.) (Brassicaceae), which is lethal to the larvae.

First, local adaptation to avoid laying eggs on …

Parent Perspectives On Barriers And Facilitators To Optimal Healthcare And Medical Screenings For Children With Down Syndrome In South Carolina, Vinita Oberoi Leedom

Theses and Dissertations

Children with Down syndrome (DS) often have co-occurring conditions affecting vision, hearing, sleeping, and more. Screenings, as recommended by the American Academy of Pediatrics (AAP), can help prevent secondary disability, and allow children to thrive. Studies have evaluated the receipt of recommended screenings, but barriers and facilitators surrounding access to screenings and care have not been described.

Parents of 24 children with DS in SC were recruited through schools and family support groups. One-hour long interviews were conducted in English and Spanish. Information was collected on health, healthcare quality, respiratory concerns, pandemic effects, access to care, and knowledge about AAP …

An Examination Of Inter-Limb Functional Asymmetry After A Fatiguing Bout Of Exercise In High Level Soccer Players, Nestor Urrea

Theses and Dissertations

INTRODUCTION: Inter-limb functional asymmetries greater than 15% have been linked to a greater incidence of injury. Moreover, asymmetries greater than 10% have shown greater risk of injury and worsened sport performance, especially for strength and power capabilities, in athletes of multiple sports. Fatigue and sex are factors that seem to influence injury rates as well as greater asymmetries between limbs. This research project aims to investigate the effects of a fatiguing bout of exercise in strength and power inter-limb functional asymmetries in male and female soccer players. It was hypothesized that strength and power asymmetries are negatively affected after a …