Life Sciences Commons^™

New Records Of Liparthrum Squamosum Blackman (Coleoptera: Curculionidae: Hypoborini) In Pennsylvania, Dani J. Firth, Lawrence E. Barringer

Insecta Mundi

Liparthrum squamosum Blackman (Coleoptera: Curculionidae: Scolytinae: Hypoborini) is reported for the first time from Pennsylvania. A single specimen was found in 2015 and four subsequent specimens were collected in 2024 through several insect trap and lure combinations. These records increase the known range for this species in the eastern United States.

Advancing Butterfly Systematics Through Genomic Analysis, Jing Zhang, Qian Cong, Jinhui Shen, Leina Song, Nick V. Grishin

The Taxonomic Report of the International Lepidoptera Survey

Within the framework of an ongoing comparative genomic study of global butterfly diversity, we construct phylogenetic trees combining all protein-coding genes assembled from the whole genome shotgun data. When viewed in the context of current taxonomy and phenotypic knowledge, the genome-wide phylogeny points to further advances in butterfly systematics, which are presented here. We assign major clades with comparable levels of genetic divergence to the same taxonomic rank and apply criteria involving relative population divergence and gene flow to define species boundaries. As a result, one genus, 13 subgenera, 62 species, and five subspecies are proposed as new (type species …

Nebraska Farm Finances In 2024: A Year Of Modest Recovery And Growing Financial Pressure, Tina N. Barrett

Cornhusker Economics

Nebraska Farm Finances in 2024: A Year of Modest Recovery and Growing Financial Pressure by Tina N. Barrett (Cornhusker Economics, May 21, 2025)

Section headers:

Net Income Recovery Falls Short of Historical Highs

Household Spending Steady Amid Income Fluctuations

Rising Debt and Shifting Balance Sheets

Liquidity Pressures Worsen

Interest Costs Add to Financial Strain

Capital Purchases Shift: Less Equipment, More Land

Crop Enterprise Analysis: Losses Mount Despite Input Savings

Crop Enterprise Analysis: Losses Mount Despite Input Savings

North Central Extension Risk Management Education Center, Part Ii: Outcomes And Impacts: Empowering Farmers, Transforming Futures, Sheila Aikanathan Johnson, Christine Lockert, Bradley D. Lubben

Cornhusker Economics

The North Central Extension Risk Management Education Center remains dedicated to helping American farmers and ranchers manage the unique risks of producing food for a global market. To date, we have funded 545 projects totaling over $20 million, reaching more than 175,500 participants. Collaboratively, these efforts reflect a growing commitment to equipping producers with the tools, knowledge, and support needed to make informed decisions, strengthen resilience, and ensure the long-term sustainability of agriculture across our region and beyond.

North Central Extension Risk Management Education Center, Part I: Helps Producers Manage Risks, Christine Lockert, Sheila Aikanathan Johnson, Bradley D. Lubben

Cornhusker Economics

Educating producers to manage risk and position their farm or ranch for future growth and success is the fundamental purpose of the Extension Risk Management Education (ERME) program.

The ERME program, funded by United States Department of Aagriculture’s National Institute of Food and Agriculture, provides training to help producers learn new strategies to manage complex and growing agricultural risks.

Established in 2001 and located at the University of Nebraska-Lincoln Agricultural Economics Department, the North Central Extension Risk Management Education Center (NCERMEC) has a long history of supporting risk management education programs serving producers in the region. NCERMEC, in conjunction …

Digitization And Archival Of Insect Collections: Literature Review, Rebekah Rogers

Department of Entomology: Distance Master of Science Projects

A slide presentation discussing how and why digitization has become a key component in the curation and care of natural history collections.

Investigating Fatty Acid Influence On West Nile Virus In Mosquitoes And Birds, Hayley Moree

Honors Program: Senior Projects (Public)

Vector-borne diseases, including Zika virus (ZIKV), Dengue virus (DENV), and West Nile virus (WNV), pose considerable global health threats, accounting for over 400 million infections and more than 700,000 deaths annually. Many vector-borne diseases are within the Flaviviridae family. Despite this, effective treatments have not been found for some flaviviruses. Flaviviruses exploit host cellular machinery to facilitate their replication and survival, reprogramming metabolic pathways, including lipid metabolism. Lipids play essential roles in the viral life cycle, providing structural components for the viral envelope, enabling membrane rearrangements for replication complexes, and supplying energy resources necessary for particle assembly.

Targeting lipid metabolism …

Novel Chloroviruses With Unique Glycans On Their Major Capsid Proteins, Cayden J. Homolka

Honors Program: Senior Projects (Public)

Chloroviruses, of the family Phycodnaviridae, are large, dsDNA viruses with a genome of 290 to 410 kb. These virus genomes are so large that they are classified as “giant viruses”. Within this genome, up to 16 tRNAs and 330 to 450 proteins are encoded. Among the massive amounts of genes and proteins are unique carbohydrate synthesis/metabolism genes, which are responsible for the glycosylation of chloroviruses major capsid proteins. In most viruses, these proteins are glycosylated by the host enzymes located in the endoplasmic reticulum. Instead, Chloroviruses encode their own glycosylation machinery; essentially decorating themselves. Furthermore, these chlorovirus glycans are unlike …

Nebline, May 2025

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

NEBLINE, May 2025

Tips on Staying Cool During the Heat of a Temper Tantrum

What’s Your BBQ IQ?

May Recipe of the Month — Air Fryer Teriyaki Beef Kabobs

Insect and Disease Control for Organic Vegetable Gardeners

May Garden Guide

Cash Rent Survey

No Matter Where You Live, Submit a Request Before You Dig!

Battling Scales in the Landscape

Three Lancaster County 4-H’ers Modeled Their Sewn Garments at Omaha Fashion Week

Ignite by 4-H 2025 National Conference

May Heart of 4-H Volunteer Award — Dayle Williamson

2024 National Index Study of 4-H Youth

Clover College, June 10–13; Registration Opens in …

Mitigating Nitrogen Losses: The Role Of Enhanced Efficiency Fertilizers In Sustainable Corn Production, Dipesh Giri

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Nitrogen (N) is essential for crop production, but its inefficient use often leads to environmental losses through nitrate leaching, ammonia volatilization, and nitrous oxide (N₂O) emissions. Enhanced efficiency fertilizers (EEFs), such as nitrification inhibitors (NIs) and urease inhibitors (UIs), have been developed to minimize these losses and improve nitrogen use efficiency (NUE). This research combined a multi-year field experiment and a controlled laboratory study to evaluate how dual-inhibitor fertilizers compare to conventional urea and single-inhibitor formulations in Nebraska cropping systems. The main objective was to understand how nitrogen inhibitors influence N availability, crop performance, and nitrogen losses across different environmental …

Improving Nitrogen Management And Corn Productivity Through Enhanced Efficiency Fertilizer And Cover Crop Integration In Nebraska Cropping Systems, Madhusudhan Adhikari

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Effective nitrogen (N) management is vital for sustaining corn yield and reducing environmental impacts in Nebraska cropping systems. Three field studies were conducted to evaluate the impact of 1) fertilizer type (anhydrous ammonia [AA] with nitrification inhibitor: enhanced efficiency fertilizer [EEF] or AA without EEF) and N application timing (pre-plant vs. split with side-dress UAN) on in-season soil N availability, crop response, nitrous oxide (N₂O) emissions, residual soil N and N use efficiencies (NUEs); 2) winter cover crop (CC) species and crop rotation variability on CC biomass production, N uptake, soil N dynamics, and cash crop performance in …

Extending Cover Crop Growing Window To Improve The Health Of Environmentally Sensitive Soils, Noshin Ara Tunazzina

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Sloping and sandy soils are highly susceptible to degradation. The health of these soils could be improved with cover crops (CCs). However, CC effectiveness in improving soil health mainly depends on CC biomass production, which is often low in temperate regions. The question is: how can we increase CC biomass production? Lengthening the CC growing window can be a potential strategy. This study assessed how 1) interseeded and terminated early (2-3 weeks before crop planting) CC, 2) interseeded and terminated late (at crop planting) CC, 3) drilled (after harvest) and terminated early CC, 4) drilled and terminated late CC, and …

Implications Of Type Iv Pilus Retraction For Dna Uptake By Acinetobacter Baumannii, Yafan Yu

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Horizontal gene transfer (HGT) via natural competence allows bacteria to incorporate extracellular DNA (eDNA) into their genomes, facilitating genetic diversity and adaptation. In Acinetobacter species, natural competence depends on DNA binding and uptake mediated by type IV pili (T4P). T4P are dynamic extracellular filaments composed primarily of the major pilin subunit PilA, along with a few minor subunits. T4P drive a range of cellular functions including twitching motility, biofilm formation, and DNA uptake, with some functions dependent on pilus retraction and others not. However, how Acinetobacter T4P bind DNA and how T4P/eDNA interactions impact biofilm formation remain unclear. Here, I …

Modifying The Comb, Rethinking The Install: Integrated Approaches To Honey Bee Colony Establishment And Winter Survival, Shelby A. Kittle

Department of Entomology: Dissertations, Theses, and Student Research

The European, or Western honey bee (Apis mellifera L.) plays a vital role in global ecosystems and agriculture, providing essential pollination services and producing valuable hive products. However, honey bee colony losses have escalated worldwide, but particularly across the United States due to various stressors, including biotic and abiotic factors, mismanagement, and environmental challenges. This thesis investigates key challenges in honey bee management and explores solutions aimed at improving colony health and productivity.

Chapter 1 provides an overview of the beekeeping industry, detailing its economic importance, services, and products, as well as the many challenges faced by beekeepers. It …

Mechanisms Of Perennial Sorghum Resistance To Sugarcane Aphid Infestation, Esha Kaler

Department of Entomology: Dissertations, Theses, and Student Research

Sorghum, Sorghum bicolor (L.) Moench, the fifth most-grown cereal crop in the world, is a versatile crop, grown across different agro-ecological regions. Sorghum has numerous uses ranging from food and forage purposes to biofuel production. Sugarcane aphid (SCA; Melanaphis sacchari) is a major threat to sorghum production, accounting for 50% to 100% yield loss under heavy infestations. Perennial sorghum can be a sustainable and climate-resilient alternative to the current cereal farming systems. The development of perennial sorghum offers numerous benefits, such as reduced economic inputs, more carbon sequestration, better soil health, and sustainable yields. We employed host plant resistance …

Epidemiological Inferences Of The Impacts Of Winter Ticks On Moose Health, Krishna S. Woerheide

Department of Entomology: Dissertations, Theses, and Student Research

The North American Moose (mooz in Anishinaabemowin, Ojibwe language, Alces alces), a culturally significant subsistence species for the Anishinaabe people, faces population decline due to calf predation, pathogen transmission, and parasitism by the winter tick (Dermacentor albipictus), all exacerbated by climate change. This study explores the impact of winter tick infestations on moose health, with a focus on natural mineral seeps as potential transmission hotspots in moose habitats within the Grand Portage Indian Reservation (Gichi Onigaming), on Isle Royale National Park (Minong), and within the 1854 Ceded Territory (greater northeastern Minnesota) - ancestral and present homelands of …

The Product Of Property Tax: How Property Tax Affects Agricultural Production In Nebraska, James L. Kriz

Department of Political Science: Dissertations, Theses, and Student Research

The question of what level of property tax is acceptable has become an increasingly prominent question within agriculture, which is shown by the rise of interest groups and bill introduction in Nebraska. As such, this paper examines how property taxes affect Nebraska's agricultural output. The study employs a quantitative analysis of all Nebraska counties over 21 years from 2000 to 2020. This study hypothesizes that as property tax rates increase, farm outputs will decrease. The study offers a new analysis in an understudied subsection of the agriculture policy literature and points towards a new direction in which the field can …

Assessing Sleep Quality In A Preclinical Model Of Low Back Pain Through Novel Noninvasive Methods, Lydia C. Saltz

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Chronic low back pain (LBP) has widespread negative impacts on quality of life. Current treatments for chronic LBP fail to provide long-term efficacy and motivate the need to understand key drivers of poor patient outcomes. Mounting evidence indicates that sleep disturbances and chronic LBP have a strong reciprocal relationship. Women may be particularly vulnerable to the negative impacts of the sleep-pain axis, exhibiting greater prevalence in both conditions. However, the relationship between sleep, pain, and sex remains understudied. Preclinical models can be used to parse out characteristic sleep features that degrade with pain, which can be further broken out by …

Screening For Chloroplast Inner Envelope-Thylakoid Membrane Contact Site Proteins, Evan W. Labrant

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Most life on Earth is dependent upon the capture of solar energy and subsequent fixation of carbon. In members of the Archaeplastida lineage these biological processes take place in semi-autonomous organelles called chloroplasts. Chloroplasts are bounded by a double membrane envelope and contain the photosynthetic membrane system, thylakoid membranes, where light capture takes place. While a great deal is known about how thylakoid lipids are synthesized and protein complexes therein are properly maintained, very little is known regarding the processes by which lipids are transported to thylakoids which lack de novo glycerolipid synthesis. We have worked to identify candidate proteins …

Ediacaran Paleocommunity Structure, Body Size Evolution, And The Rise Of Complex Animals, Matthew Philip Craffey

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

The Ediacaran period (635-538ma) is a key interval in the evolution of life as it records the first macroscopic animal communities. Major extinction pulses appear to have altered the composition of the global marine biosphere between each sub-interval of the Ediacaran Period: the Avalon, White Sea, and Nama assemblage Zones. While considerable attention has been paid to the causes of these extinction pulses, less has been afforded to the consequences of Ediacaran biotic turnover for community organization and function. Moreover, it is unclear how body size - the defining trait of macrofauna - affected the transition between animal groups across …

Influence Of Biotic And Abiotic Factors On Decomposition And The Determination Of Time Since Death: Applications Of Forensic Entomology And Taphonomy, John Oladapo Obafunwa

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

The determination of the postmortem interval (PMI) is essential for the investigation and successful prosecution of suspected cases of homicide. While the forensic pathologist might be able to draw fairly accurate conclusions during the initial 24–36 hours, beyond this period, the assistance of other experts like forensic entomologists and taphonomists would be required. The present study was designed to observe the postmortem changes in Sus scrofa placed in a cage in Nebraska, located in the American Central Great Plains. The setting is in an open grassland in late spring to early summer. The observed variables with respect to biotic and …

Integrating Genomic And Phenomic Breeding Tools For Wheat Stem Sawfly Resistance In Hard Winter Wheat, Sydney E. Graham

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

The wheat stem sawfly (WSS; Cephus cinctus) is an insect pest that affects wheat (Triticum aestivum) in the Northern Great Plains. This pest causes stem cutting that makes the plants susceptible to lodging and results in up to 30% yield loss. The primary management strategy for producers is utilizing host plant resistance, for which the main trait is stem solidness. This trait increases the pith thickness and provides resistance to the WSS by increasing larval mortality and reducing stem cutting. While stem solidness is largely controlled by a known major gene (SSt1), the phenotypic expression is also …

Spatial Ecology And Predator–Prey Interactions Of Mountain Lions In California's San Francisco North Bay, Jacob A. Harvey

School of Natural Resources: Dissertations, Theses, and Student Research

Understanding spatial ecology and predator-prey interactions are central to wildlife ecology and conservation. We tracked mountain lions with GPS telemetry to evaluate space use, predator-prey interactions, and resource selection in California’s San Francisco North Bay, USA. In Chapter 1, we evaluated the influence of human disturbance on home range size, prey composition, and kill rates on black-tailed deer (Odocoileus hemionus columbianus) across an urban-rural gradient. Both males and females increased home range size with increasing development at low to moderate levels of development, but female home range size stabilized across greater proportions of development while male home range …

Maggot Mass Effect On The Development And Survival Of Forensic Blow Flies, Akomavo Fabrice Gbenonsi

School of Natural Resources: Dissertations, Theses, and Student Research

Forensic entomology scientists use blow flies' developing patterns to estimate the postmortem interval (PMI). However, larval aggregations produce metabolic heat, resulting in complex microclimates that may change growth timetables. This study investigates how larval density and associated temperature changes affect the development and survival of two forensically essential blow fly species, Lucilia sericata and Calliphora vicina.

This study examined how larval density and associated thermal profiles affect the development of both blow fly species. Using a split-plot design, we tested four larval densities at 25°C and 30°C, with temperature gradients measured via thermocouple at four mass positions three times …

Assessing Spatial And Temporal Variation In Photoprotective Responses Of Deciduous And Evergreen Tree Canopies With Leaf Spectroscopy, Alexander Piper

School of Natural Resources: Dissertations, Theses, and Student Research

Environmental conditions frequently prevent carbon fixation by plants, leading to the absorption of excess light that can damage photosynthetic machinery if not dissipated. To do so, plants utilize several photoprotective mechanisms, some detectable remotely using Photochemical Reflectance Index (PRI). Two components of PRI correspond to the facultative engagement of the xanthophyll cycle (ΔPRI) and constitutive changes in xanthophyll pigment pool sizes (PRI0), representing distinct mechanisms regulating shorter and longer-term photoprotection, respectively. Our understanding of interspecific and intraspecific variation in these mechanisms is limited, primarily because PRI components are often not clearly distinguished. This study aimed to assess the variation in …

Assessment Of Sampling Gears For Bigheaded Carp In Mid-Order Prairie Rivers In Nebraska, Brett M. Anderson

School of Natural Resources: Dissertations, Theses, and Student Research

Silver Carp Hypophthalmichthys molitrix and Bighead Carp H. nobilis (collectively bigheaded carp) have invaded tributaries to the Missouri River and Platte River in Nebraska, USA. Information pertaining to efficient sampling protocols for assessing bigheaded carp populations as well as benefiting removal efforts is needed. Herding is a method used to increase the capture efficiency and detection probability of adult bigheaded carp by simultaneously using active and passive sampling gears. During June-July 2023, four herding techniques were assessed regarding their ability to elicit directional movement past an enclosure gate. The combination of sound and electrofishing resulted in the highest mean …

Leveraging Historical Data For Estimating Genetic Gain And Implementing Genomic Selection In A Student Led Barley Breeding Program, Sydney Graham

Department of Statistics: Dissertations, Theses, and Student Research

In Nebraska, winter feed barley presents an emerging market for producers and an opportunity to diversify cropping systems. The University of Nebraska Barley Breeding Program aims to develop high-yielding, winter-hardy varieties. A unique aspect of this program is that doctoral students serve as barley breeders and are responsible for crossing, data collection, and advancement decisions. While this provides hands-on experience for the students, the impact of student leadership has not been examined.

This study used a historical data set to evaluate the realized genetic gain of the breeding program, and as a training population for genomic selection. The dataset consisted …

Biological Reduction Of Perchlorate In Martian Regolith Simulant Using Azospira Oryzae, Brianna Bernhardt

Honors Program: Senior Projects (Public)

Establishing a sustainable human presence on Mars will require in-situ resource utilization (ISRU) to reduce costly and inefficient resupply missions. Despite containing many of the requirements for agriculture, Martian regolith is contaminated with toxic levels of perchlorate (ClO₄^-), threatening human health and agricultural success. Biological remediation using perchlorate-reducing bacteria offers a promising approach to address these challenges. This study investigated the perchlorate-reducing ability of Azospira oryzae, a facultative anaerobe capable of converting ClO₄^- to chloride (Cl^-) through dissimilatory respiration. A. oryzae was cultured in Martian regolith simulant (MGS-1) amended with 0.0%, 0.5%, …

Sodium And Potassium In Health And Cellular Function, Jordyn Branz

Honors Program: Senior Projects (Public)

Sodium (Na⁺) and potassium (K⁺) are essential electrolytes that regulate numerous physiological processes. These include cell function, nerve transmission, and muscle contraction. The Na+/K+-ATPase plays a critical role in maintaining the balance of these ions by actively transporting three sodium ions out of the cell and two potassium ions into the cell against their concentration gradients. This process is vital for maintaining the resting membrane potential and supporting the function of excitable cells. The Na+/K+-ATPase consists of two main subunits, with the alpha-subunit being primarily responsible for ion transport activity. Isoforms alpha1, alpha2, and alpha3 are tissue-specific and contribute to …

Advancing Mitochondrial Dna Visualization And Dynamics In Plants: Application Of The Mt-Hi-Ness Fluorescent Marker, Madeleine H. Faber

Honors Program: Senior Projects (Public)

Mitochondrial DNA (mtDNA) plays a crucial role in cellular energy production and inheritance, yet its dynamics and exchange remain poorly understood in plant systems due to limitations in visualization techniques. Traditional visualization methods such as exogenous DNA stains and fluorescent protein markers present challenges in specificity and live-cell imaging. To address this challenge, we adapted the MT-HI-NESS fluorescent marker, originally developed for mammalian cells, for use in plant mitochondrial studies. Using Arabidopsis thaliana as a model system, we employed mitochondrial targeting sequences (IVD and AOX) to ensure accurate localization and to confirm mtDNA binding specificity. The validation process included fluorescence-based …