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Articles 571 - 577 of 577
Full-Text Articles in Life Sciences
The Identification Of Cis-Ii, I2-Methylene-2-Hydroxyoctadecanoic Acid From Thiobacillus Thiooxidans, H. W. Knoche, J. M. Shively
The Identification Of Cis-Ii, I2-Methylene-2-Hydroxyoctadecanoic Acid From Thiobacillus Thiooxidans, H. W. Knoche, J. M. Shively
Department of Biochemistry: Faculty Publications
A polar fatty acid has been observed as a component of an
ornithine-containing lipid of Thiobacillus thiooxidans. A
comparison of thin layer chromatographic mobilities of reference
compounds to those of the natural acid and its derivatives
suggested that the acid was a 2-hydroxy fatty acid.
The presence of a cyclopropane function in the acid was
indicated by l4e-Iabeling experiments and infrared spectroscopy.
Mass spectrometry of the methyl ester and the acetylated
methyl ester of the natural acid provided a molecular
weight for the acid. Equivalent chain lengths were determined
for the natural acid, the acid obtained by oxidative
decarboxylation of …
Carbohydrate And Lipid Metabolism During Germination Of Uredospores Of Puccinia Graminis Tritici, J. M. Daly, H. W. Knoche, M. V. Wiese
Carbohydrate And Lipid Metabolism During Germination Of Uredospores Of Puccinia Graminis Tritici, J. M. Daly, H. W. Knoche, M. V. Wiese
Department of Biochemistry: Faculty Publications
Uredospores of Puccinia gramintis (Pers.) tritici (Eriks. and Henn.) were uniformly labeled with 14C by permitting the host (Triticuml aestivum L.) to carry out photosynthesis in 14CO2 during the process of spore production by the obligate parasite. The use of 14C labeled spores provided advantages in a study of the utilization of endogenous substrates at frequent intervals with small amounts of spores under conditions conducive to germination.
Concerning The Site Of Nitrogen Absorption In Rats Fed Autoclaved Or Raw Soybean Oil Mea, Raymond Borchers
Concerning The Site Of Nitrogen Absorption In Rats Fed Autoclaved Or Raw Soybean Oil Mea, Raymond Borchers
Department of Biochemistry: Faculty Publications
Carroll, Hensley, and Graham (1) have concluded that much of the nitrogen absorption in rats fed raw soybean oil meal must take place in the cecum. This conclusion was reached from data showing that the apparent digestibility of raw soybean nitrogen in the terminal 20% of the small intestine was 32.65%, whereas in the feces the value was 76.96%. Values reported, for apparent digestibility of heated soybean nitrogen were 78.66% and 81.78%, respectively. This observation presented a notable advance in explaining the lower nutritive value of raw soybeans compared with autoclaved soybeans. It therefore seemed advisable to repeat this work …
Riboflavin Production By Molds, George L. Peltier, Raymond Borchers
Riboflavin Production By Molds, George L. Peltier, Raymond Borchers
Department of Biochemistry: Faculty Publications
Very little information is at hand regarding the ability of molds to synthesize riboflavin. Few citations pertaining directly to riboflavin production by true
molds are available. Pontovich (1943) found as much as 2 mg riboflavin per g
of Aspergillus flavus mycelium. Tanner et at. (1945) determined the quantity of
riboflavin in the submerged fermentation media of Penicillium chrysogenum.
The highest value found was 1. 36 mg per ml. The primary purpose of this study
was to screen several hundred isolates, recently obtained from soil, crop residues,
and composts, for their ability to produce riboflavin on a wheat bran substrate.
Threonine Deficiency In Hydrolysates Of Zein Prepared By Autoclaving, Raymond Borchers, John R. Totter, Clarence P. Berg
Threonine Deficiency In Hydrolysates Of Zein Prepared By Autoclaving, Raymond Borchers, John R. Totter, Clarence P. Berg
Department of Biochemistry: Faculty Publications
In a previous paper (Borchers and Berg, 1942) we showed that
autoclaving zein with sulfuric acid longer than necessary for complete
hydrolysis causes destruction and racemization; either of
these might account for the failure of such a hydrolysate to
promote growth in young rats when substituted for a hvdrolysate,
prepared by refiuxing, in a diet which produced moderate growth.
It seemed to us that this deterioration in the dietary protein might
well be the result primarily of essential amino acid deficiencies
which could be detected and overcome by appropriate supplementation.
Because threonine is known to be present in zein in …
Tryptophane Metabolism X. The Effect Of Feeding 1(-)-, Dl-, And D(+)-Tryptophane, D(-)And Dl-(Β-3-Indolelactic Acid,(Β-3-Indolepyruvic Acid, And L(-)Kynurenine Upon The Storage Of Liver Glycogen And The Urinary Output Of Kynurenic Acid, Kynurenine, And Total Acetone Bodies, Raymond Borchers, Clarence P. Berg, Newton E. Whitman
Tryptophane Metabolism X. The Effect Of Feeding 1(-)-, Dl-, And D(+)-Tryptophane, D(-)And Dl-(Β-3-Indolelactic Acid,(Β-3-Indolepyruvic Acid, And L(-)Kynurenine Upon The Storage Of Liver Glycogen And The Urinary Output Of Kynurenic Acid, Kynurenine, And Total Acetone Bodies, Raymond Borchers, Clarence P. Berg, Newton E. Whitman
Department of Biochemistry: Faculty Publications
The amount of tryptophane required for maintenance and growth is small (2). When an excess is supplied to some animals, kynurenic acid is excreted (3) and, under certain conditions, kynurenine also (4). Although these are quantitatively the most important of the known metabolic products of tryptophane, the amounts excreted in extensive tests in the dog and rabbit have usually accounted for less than half, more often for less than a third, of the tryptophane administered (4-6).
The Effect Of Conditions Of Hydrolysis And Of Prolonged Heating Upon The Optical Rotation Of Sulfuric Acid Hydrolysates Of Zein, Raymond Borchers, Clarence P. Berg
The Effect Of Conditions Of Hydrolysis And Of Prolonged Heating Upon The Optical Rotation Of Sulfuric Acid Hydrolysates Of Zein, Raymond Borchers, Clarence P. Berg
Department of Biochemistry: Faculty Publications
Some time ago we observed that a sulfuric acid hydrolysate of zein prepared in an autoclave at 165˚ did not support growth in young rats when it was supplemented with certain amino acids, even though the same amino acids, added to a hydrolysate prepared by refluxing, permitted moderate growth. Since the optical rotation of the autoclaved hydrolysate was lower than that of the refluxed, either destruction or racemization of essential constituents, or both, might have occurred. Loss of amino nitrogen and production of extra ammonia upon prolonged autoclaving of proteins with acids have been noted by Van Slyke (1912) …