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Full-Text Articles in Life Sciences
Inheritance Of Freezing Resistance In Tuber-Bearing Solanum Species: Evidence For Independent Genetic Control Of Nonacclimated Freezing Tolerance And Cold Acclimation Capacity, Julie M. Stone, Jiwan P. Palta, John B. Bamberg, Laurie S. Weiss, James F. Harbage
Inheritance Of Freezing Resistance In Tuber-Bearing Solanum Species: Evidence For Independent Genetic Control Of Nonacclimated Freezing Tolerance And Cold Acclimation Capacity, Julie M. Stone, Jiwan P. Palta, John B. Bamberg, Laurie S. Weiss, James F. Harbage
Department of Biochemistry: Faculty Publications
Frost or winter survival is regarded as a complex trait with polygenic inheritance. Two maijor components of this survival in crop plants are freezing tolerance in the nonacclimated state and cold acclimation capacity. To date researchers have not distinguished the two components as separate heritable traits. The mode of inheritance of these two traits was investigated in F1 and backcross populations of two wild diploid potato species (Solanum commersonii and Solanum cardiophyllum) exhibiting extremes of freezing tolerance and acclimation capacity. Precise assessment of these two traits allowed distinction of small but significant differences among genotypes. The two traits …
Shared Thematic Elements In Photochemical Reaction Centers, John H. Golbeck
Shared Thematic Elements In Photochemical Reaction Centers, John H. Golbeck
Department of Biochemistry: Faculty Publications
The structural, functional, and evolutionary relationships between photosystem II and the purple nonsulfur bacterial reaction center have been recognized for several years. These can be classified as "quinone type" (type I) photosystems because the terminal electron acceptor is a mobile quinone molecule. The analogous relationship between photosystem I and the green sulfur bacterial (and heliobacterial) reaction centers has only recently become dear. These can be dcassified as "iron-sulfur type" (type I) photosystems because the terminal electron acceptor consists of one or more bound iron-sulfur clusters. At a fundamental level, the quinone type and ironsulfur type reaction centers share a common …
Mutational Analysis Of The Structure And Biogenesis Of The Photosystem I Reaction Center In The Cyanobacterium Synechocystis Sp. Pcc 6803, Lawrence B. Smart, Patrick V. Warren, John H. Golbeck, Lee Mcintosh
Mutational Analysis Of The Structure And Biogenesis Of The Photosystem I Reaction Center In The Cyanobacterium Synechocystis Sp. Pcc 6803, Lawrence B. Smart, Patrick V. Warren, John H. Golbeck, Lee Mcintosh
Department of Biochemistry: Faculty Publications
We have utilized the unicellular cyanobacterium Synechocystis sp. PCC 6803 to incorporate site-directed amino acid substitutions into the photosystemn I (PSI) reactioncenter protein PsaB. A cysteine residue (position 565 of PsaB) proposed to serve as a ligand to the [4Fe-4S] center Fx was changed to serine, histidine, and aspartate. These three mutants- C565S, C565H, and C565D-all exhibited greatly reduced accumulation of PSI reaction-center proteins and failed to grow autotrophically, indicating that this cysteine most likely does coordinate Fx, which is crucial for PSI biogenesis. Interestingly, the strain C565S accumulated significantly more PSI than the other two cysteine …
Exopeptidase Catalyzed Site-Specific Bonding Of Supports, Labels And Bioactive Agents To Proteins, Fred W. Wagner, Thomas R. Coolidge, Sheldon M. Schuster, Jay Stout, Dwane E. Wylie, Klaus Breddam, William Lewis
Exopeptidase Catalyzed Site-Specific Bonding Of Supports, Labels And Bioactive Agents To Proteins, Fred W. Wagner, Thomas R. Coolidge, Sheldon M. Schuster, Jay Stout, Dwane E. Wylie, Klaus Breddam, William Lewis
Department of Biochemistry: Faculty Publications
An auxiliary substance such as a label, support, or bioactive agent is attached to a protein at a site that is remote from the active site of the protein by the use of exopeptidase and a nucleophile which is an amino acid, amino acid derivative, amine or alcohol. In one embodiment, the nucleophile is attached to the carboxy terminus of a protein by catalysis with exopeptidase to form an adduct and then the adduct or its combination with a linker arm is bound to the auxiliary substance. In another embodiment, the auxiliary substance or its combination with a linker arm …