Life Sciences Commons^™

Relationship Of Weed Control And Soil Ph To No-Tillage Corn Yields, J. J. Kells, C. E. Rieck, Robert L. Blevins, Charles H. Slack

Agronomy Notes

Atrazine and simazine are used for selective control of a broad spectrum of weeds in corn. Over 80% of the U.S. corn production is treated with one or the other of these two s-triazine herbicides. In Kentucky they are used annually on over 800,000 acres of corn, including over 200,000 acres of no-tillage corn. When added to the soil these compounds are ultimately degraded to non-phytotoxic compounds. The rate of degradation is dependent upon the physical, chemical, and biological properties of the soil. Although atrazine and simazine are chemically similar, simazine is considered to degrade slightly slower than atrazine after …

Role Of A Nonimmunoglobulin Cell Surface Determinant In The Activation Of B Lymphocytes By Thymus-Independent Antigens*, Subbarao Bondada, Donald E. Mosier, Aftab Ahmed, James J. Mond, Irwin Scher, William E. Paul

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Lyb 5 is a B-cell alloantigen which is expressed on 50-60% of B cells. It was defined originally on the basis of cytotoxicity. We have described a new reactivity within the anti-Lyb 5 serum on the basis of selective inhibition of antibody responses in vitro by this antiserum in the absence of complement. This inhibitory activity of anti-Lyb 5.1 serum appears to be due to recognition of antigenic determinants different from the prototype antigens detected in the cytotoxicity assay. Anti-Lyb 5 serum incorporated into spleen cell cultures selectively inhibits antibody responses to a class of thymus-independent antigens (TI-2) previously characterized …

Tritium (3h) Radiolabeling Of Protein A And Antibody To High Specific Activity: Application To Cell Surface Antigen Radioimmunoassays, Ronald L. Wilder, Catherine C. Yuen, Subbarao Bondada, Virgil L. Woods, Cornelius B. Alexander, Rose G. Mage

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Staphylococcal protein A and several different immunoglobulins have been radio-labeled to high specific activities (> 106 cpm/pg) by reductive methylation with tritiated (3H) sodium borohydride. The proteins retain excellent functional and antigenic properties. The utility of these reagents in a variety of assays for cell surface antigens is illustrated. The results indicate that this radiolabeling procedure may become the method of choice for many cell surface and solution immunoassays.

A Computer Model For Evaluating Corn Harvesting, Handling, Drying And Storage Systems, Thomas C. Bridges, Otto J. Loewer Jr., John N. Walker, Douglas G. Overhults

Biosystems and Agricultural Engineering Faculty Publications

No abstract provided.

Effects Of Polychlorinated Biphenyl Compounds And Proposed Pcb-Replacement Products On Embryo-Larval Stages Of Fish And Amphibians, Wesley J. Birge, Jeffrey A. Black, Albert G. Westerman

KWRRI Research Reports

Static renewal bioassays were performed with four polychlorinated biphenyls, including Capacitor 21 and Aroclors 1016, 1242, and 1254. Each compound was used to treat embryo-larval stages of the rainbow trout (Salmo gairdneri), channel catfish (Ictalurus punctatus), goldfish (Carassius auratus), redear sunfish (Lepomis microlophus), leopard frog (Rana pipiens), Fowler's toad (Bufo fowleri), and American toad (Bufo americanus). Developmental stages of the trout were the most sensitive, with LC₅₀ 's at 4 days posthatching of 0.3, 1.0, 1.1, and 1.6 μg/l for Aroclors 1254, 1242, 1016, and …

Influence Of Nitrogen Fertilization On The Quality And Quantity Of Streamflow From A Forested Watershed, George B. Coltharp, Michael T. Shearer, Everett P. Springer, Robert F. Wittwer

KWRRI Research Reports

This project was designed to determine the effects of nitrogen fertilization on the quality and quantity of streamflow eminating from an eastern hardwood forest watershed. A 40.67 ha watershed, located in mountainous eastern Kentucky, was aerially fertilized in late April 1975. The forest stand was principally oak, hickory, and yellow poplar, 50 - 55 years of age and in a relatively undisturbed condition. A helicopter applied anunonium nitrate at a rate of 504 kg/ha. Because a large part of applied nitrogen fertilizer ends up in the highly mobile nitrate nitrogen.form, this is the principal ion monitored in this study. No …

Zinc Fertilization Of Corn In Kentucky, W. W. Frye, H. F. Miller, Lloyd W. Murdock, D. E. Peaslee

Agronomy Notes

Of the agronomic crops, corn is especially sensitive to zinc deficiency. The major symptom of zinc deficiency in corn is broad white stripes in the leaves at or near the growing point in the early growth stages of the plant. It has been referred to as "white bud" disease because of the characteristic whitish area on one or both sides of the midrib near the base of new leaves. It can be seen as a new leaf unfolds from the whorl. Growth is stunted resulting in shorter than normal internodes. The pith of the stalk may become darkened at the …

Urea As A Source Of Fertilizer Nitrogen For Crops In Kentucky, Kenneth L. Wells, Lloyd Murdock, H. F. Miller

Agriculture and Natural Resources Publications

Kentucky farmers are finding proportionately more nitrogen being marketed each year in the chemical form of urea. This trend has resulted from a greatly expanded production capacity for urea by basic nitrogen producers. Judging from present projections, this increase in the production of urea is likely to continue. Although urea nitrogen has been marketed in Kentucky for several years, there are still many questions asked by farmers about its agronomic characteristics.

Foliar Fertilization Of Soybeans - 1977, Lloyd W. Murdock, Preeda Boon-Long, Dennis B. Egli, Kenneth L. Wells

Agronomy Notes

Field investigations on the effect of foliar fertilization were continued on soybeans during 1977. Results from our 1976 studies have previously been published.

In 1976 we tested a material produced by TVA. We used the same TVA-material in 1977 and in addition, included a commercially available product (Folian^tm, manufactured by Allied Chemical Corporation).

Response Of Different Crops To Various Rates Of Furnace Ash Applied As A Soil Amendment, Lloyd W. Murdock, George Everette

Agronomy Notes

Furnace ash from coal fired electrical generating steam plants has increased greatly in the last decade with the increased demand for electricity and increased restrictions on air pollution. The large volumes of ash are presenting disposal and containment problems for management of electrical generating plants. Property adjacent to the generating plants has been exposed to fly ash settling from the smoke stacks and ash over flowing from holding structures. The effects of the material on the land and its production are unknown and concern property owners.

Studies On The Role Of Solar Ultraviolet Radiation In "Natural" Water Purification By Aquatic Ecosystems, John Calkins, Jeanne A. Barcelo, Perry Grigsby, Stephen Martin

KWRRI Research Reports

The possibility that pollution might deplete the stratospheric ozone layer and intensify solar UV at the earth's surface focuses attention on the role of solar UV in the various ecosystems at the earth's surface. Previous studies suggested that solar UV might contribute to bacterial die off in wastewater and the studies reported here were directed toward elucidating the action of solar UV in "natural" waters.

It has been assumed that solar UV action on aquatic ecosystems can be evaluated (using proper models) on the basis of the following four independently measurable quantities: I) the intensity of solar UV at the …

Manganese Fertilization Of Soybeans, Lloyd W. Murdock, Harold F. Miller, Doyle Peaslee, Wilbur Frye

Agronomy Notes

Only a small quantity of manganese is essential for plant growth however under certain soil conditions even this is not available to the plant. Depending on severity, manganese deficiency will retard plant growth and may drastically decrease yields. A deficiency of manganese will affect the photosynthesis system of the plant. The visual symptoms of a manganese deficiency on soybeans is interveinal chlorosis of the leaves. The veins remains green until the chlorosis nears the white stage then they also lose their color.

Boron Fertilization Of Corn In Kentucky, Lloyd W. Murdock, Kenneth L. Wells, H. F. Miiller

Agronomy Notes

The need for use of boron (B) for alfalfa production has been recognized for many years in Kentucky and is presently recommended for general use as an annual topdressing on alfalfa fields. Use of B is also recommended in Kentucky for red clover fields which are to be harvested for seed. In recent years, there has been interest in use of fertilizer B for good corn production in Kentucky. Plant available B is greatly affected by soil pH and decreases with increasing soil pH. Because of this, some states recommend use of B on corn grown on soils with a …

Alfalfa Response To Varying Rates Of Phosphorus And Potassium Fertilization On Deep, Red, Limestone - Derived Soils Of The Pennyroyal Area In Kentucky, H. C. Vaught, Kenneth L. Wells, K. L. Driskill

Agronomy Notes

The Western Pennyroyal area of Kentucky consists largely of deep, well-drained limestone-derived soils occurring on an undulating to rolling landscape. A thin loess mantle overlies the limestone residuum in many areas. Soils on these landscapes are capable of good crop production, providing the basis of the rather intensive grain-hay- tobacco-livestock farming systems common to the Pennyroyal area. Because of the well-suited physical characteristics of these soils, alfalfa is a very important hay crop to this area.

Field Tests Of Some Liming Treatments For Growing Burley Tobacco On Acid Soils, A. M. Wallace, Kenneth L. Wells, J. L. Sims, Paul Gray

Agronomy Notes

A major problem in the production of tobacco on acid soils in Kentucky is manganese toxicity, despite the fact that it can be easily prevented. Since manganese toxicity is caused by high levels of soil acidity, prevention of soil acidity by liming is the best method of control. And soil testing is the only way to determine how acid a field has become.

Southern Regional Beef Cow-Calf Handbook: Hay Feeding Systems, Garry D. Lacefield, J. Kenneth Evans, Joe Burns

Agriculture and Natural Resources Publications

Forages (pasture, hay and silage) constitute the most feed consumed by beef cattle. The most convenient approach in producing feed is to provide quality pastures during as much of the year as possible and permit animals to graze growing forage to the maximum. To provide a year-round feed supply, however, one must usually rely on stored feed when growth is inadequate to meet animal needs.

Hay is the most common source of stored feed used in most beef cattle operations. Nutrients provided in hay harvested at the proper stage of plant growth and undamaged by weather usually cost less to …

Relationship Between Soil Ph And Yield Of Burley Tobacco, J. L. Sims, W. O. Atkinson, Kenneth L. Wells

Agronomy Notes

Historically, use of soil with a pH of 5.6 to 5.8 has been recommended for producing best quality burley tobacco. This recommendation for a relatively low pH resulted from the reported increases in the incidence of certain diseases, notably black root rot (Thielaviopsis basicola) and black shank (Phytophthora parasitica), in soils with pH greater than 5.8. Additionally, some researchers have reported undesirable chemical properties of cured leaf grown on soils that had been limed. However, workers at the Kentucky Agricultural Experiment Station generally have reported favorable effects from use of lime on both yield and quality of tobacco grown in …

Foliar Fertilization Of Soybeans, Kenneth L. Wells, Dennis B. Egli, C. E. Wyatt, John Kavanaugh

Agronomy Notes

Research reported by Iowa State University during the winter of 1975-76 suggested that a means of increasing soybean yields by use of a specific foliar fertilizer had been demonstrated (1976). Theory proposed to explain the reported responses was as follows: Up to the seed filling period in soybean growth, soybean roots and activity of nitrogen-fixing bacteria contained in root nodules are capable of supplying the necessary nutrients to meet the plants demands. However, as seed filling begins root growth stops and nodule activity declines. This results in nutrients being redistributed from leaves to seeds and a reduction in rate of …

Effect Of Lime And Source Of Nitrogen Fertilizer On Yield And Chemical Composition Of Burley Tobacco, J. L. Sims, W. O. Atkinson, Kenneth L. Wells

Agronomy Notes

Current trends in the fertilizer industry indicate a shift to greater production of ammonium forms of nitrogen fertilizer in comparison to nitrate forms. Reasons for these trends are many, but both economic and energy conservation are major factors. Generally, ammonium forms are produced with less, total energy and have higher nitrogen contents that permits lower production and transportation costs per unit of N. Agronomically, ammonium forms are less readily leached from soils, but produce acid as they are converted to nitrates by soil microorganisms. Thus, ammonium sources of nitrogen have both advantages and disadvantages as fertilizers for crops.

Growth Response Of Kenhy Fescue To Nitrogen Fertilizer, Kenneth L. Wells, Robert C. Buckner, George Armstrong, Paul Gray, C. E. Wyatt

Agronomy Notes

Kenhy fescue is a new, improved variety of tall fescue which has recently been released by the University of Kentucky Agricultural Experiment Station and the U.S.D.A. Agricultural Research Service (see University of Kentucky publication AGR-60, "Kenhy A New Tall Fescue Variety"). Seed of this variety should become available to farmers in limited quantities in the summer 1977. The purpose of this report is to provide information on how this newly developed fescue variety produces as affected by time and rate of nitrogen application.

Vertical Drainage In Field Cores, Larry G. Wells, R. W. Skaggs

Biosystems and Agricultural Engineering Faculty Publications

No abstract provided.

Inactivation Of Brain Hexokinase By An Adenosine 5'-Triphosphate Analog, Subbarao Bondada, Umakant W. Kenkare

Microbiology, Immunology, and Molecular Genetics Faculty Publications

An analog of ATP, 6-mercapto-9+nribofuranosylpurine 5’-triphosphate (SH-TP), functions as a phosphoryl donor for the reaction catalyzed by hexokinase. Incubation of the enzyme with millimolar concentrations of this reagent led to its rapid inactivation. However, the corresponding monophosphate derivative of this reagent was much less effective. A plot of the initial rate of inactivation versus the concentration of SH-TP exhibited saturation, suggesting the formation of a reversible complex between the enzyme and SH-TP prior to the inactivation reaction. The dissociation constant of this enzyme-inhibitor complex was the same as the K, of this reagent with respect to hexokinase in the phosphoryl …

Modeling Soil Water Movement Into Plant Roots, D. C. Slack, C. T. Haan, Larry G. Wells

Biosystems and Agricultural Engineering Faculty Publications

Mathematical model was developed which describes uptake of water by plant roots as a function of leaf and soil water potentials. The model was used to estimate transpiration from corn grown in a controlled environment under soil drying conditions. The model predicted daily transpiration quite well for the period modeled.

Reaction Of Brain Hexokinase With Tetranitromethane: Oxidation Of Essential Thiol Groups, Subbarao Bondada, Umakant W. Kenkare

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Treatment of bovine brain mitochondrial hexokinase with a fivefold molar excess of tetranitromethane (TNM) at pH 8.0 results in a complete loss of activity of the enzyme. Spectral measurements and amino acid analysis showed that only cysteinyl residues were modified by TNM under these conditions. The products of the reaction of TNM with the enzyme were cysteic acid and disulfides as found by amino acid analysis of the carboxymethylated hexokinase. Failure of 2-mercaptoethanol to reverse the effect of TNM suggested that the thiol group that is oxidized to cysteic acid is the one essential for enzyme activity. Substrates glucose and …

Biological And Chemical Evaluation Of The Aquatic Environment Of Selected Undeveloped Kentucky Lake Embayments, Marshall Gordon, Morgan E. Sisk

KWRRI Research Reports

This report describes research involving biological and chemical analysis of two undeveloped embayments on Kentucky Lake, namely Anderson and Vickers Bays. Field and laboratory studies were made to assess current biotic standing crops, limnological conditions, levels of inorganic and organic pollutants in the embayments.

Renovating Grass Fields With A Renovation Seeder, Edward M. Smith, Robert L. Fehr, Garry D. Lacefield, J. Kenneth Evans

Agriculture and Natural Resources Publications

Putting legumes into grass dominant pasture and hay fields through renovation is certainly not a new idea in Kentucky. Renovation has been an important forage improvement practice for the past twenty years. What is relatively new is the power tillage technique of introducing legumes into grass dominant sods.

Comparisons Of Nitrogen Fertilizer Sources Under Kentucky Soil And Climatic Conditions, Kenneth L. Wells, Lloyd W. Murdock, Harold F. Miller

Agronomy Notes

A summary of yield data comparing different nitrogen fertilizer sources in field experiments conducted by the Agronomy Department staff should provide information that will help answer some of the inquiries regarding the comparative value of some of the nitrogen fertilizers being marketed in Kentucky. Since the nitrogen rates per acre shown in all tables are ACTUAL NITROGEN the COST PER POUND OF NITROGEN in the different materials is an important consideration when evaluating the various products.

The Effects Of Swathing Wheat On The Date Of Harvest, Yield And Seed Quality, Dennis M. Tekrony, Alan Phillips, Ted Howard

Agronomy Notes

In 1975 approximately 350,000 acres were double-cropped using small grain and soybeans in Kentucky. Due to the low acreage of winter barley (48,000 acres) most of the soybeans were either planted by no-till or conventional means following winter wheat. The major problem with winter wheat in a double-cropping system is that the date of planting for soybeans is delayed. Research at the West Kentucky Experiment Station at Princeton indicates that this delay can reduce soybean yields from 7 to 11 bushels per acre depending upon the soybean variety used. The research at Princeton also indicates no reduction in soybean yields …

Computer Layout And Design Of Grain Storage Facilities, Otto J. Loewer Jr., Thomas C. Bridges, Douglas G. Overhults

Biosystems and Agricultural Engineering Faculty Publications

No abstract provided.

Facility Costs Of Centralized Grain Storage Systems Utilizing Computer Design, Otto J. Loewer Jr., Thomas C. Bridges, Douglas G. Overhults

Biosystems and Agricultural Engineering Faculty Publications

The item considered most often when estimating the cost of a grain storage facility is capacity. Of equal or greater importance is daily harvest rate. Other important considerations include drying method, materials handling equipment, energy cost, facility arrangement, labor requirements, grain quality and management ability. Only through a comparison of facilities which offer similar capacity, capability and convenience can the purchaser obtain his best system.