Life Sciences Commons^™

Forage News [1998-05], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Notes from the Kentucky Grazing School
• Beth Prewitt Wins Award at National Forage Meeting
• Balage is a Good Harvest Option
• Effect of Nitrogen on Legumes in Pastures
• Statewide Beef-Forage Field Day Planned
• Harvest Decisions Affect Quality
• Upcoming Events

Diffuse Reflectance Changes During The Culture Of Cottage Cheese, Frederick Alan Payne, R. Carol Freels, Sue E. Nokes, Richard S. Gates

Biosystems and Agricultural Engineering Faculty Publications

A sensor for measuring diffuse reflectance of milk during the typical 6-h culture of cottage cheese was installed in a local manufacturing facility. Diffuse reflectance was found to increase slowly during the first three hours of the culture and increase rapidly toward the end of fermentation. The correlation between parameters generated from the diffuse reflectance profile and cutting time was sufficient to develop an algorithm for cutting time prediction. An algorithm incorporating t_max (time from adding culture to the maximum rate of change in reflectance) and slope of the reflectance curve at t_max predicted the operator selected cutting …

Forage News [1998-04], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Henning Elected AFGC President
• CRP - Native Plant Establishment
• Influence of Soil Texture on Alfalfa Autotoxicity
• Grazing Management Effects on Yield and Persistence of Alfalfa
• Length of the Grazing Period: Does it Really Matter?
• Coated Alfalfa Seed Establishment and Yield Trials in Commercial Fields in Central Minnesota
• Alfalfa Hay Storage Losses as Influenced by Bale Type and Storage Method
• Upcoming Events

Cloning And Developmental Expression Of Pea Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Large Subunit N-Methyltransferase, Robert L. Houtz

Horticulture Faculty Patents

The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) εN-methyltransferase (protein methylase III or Rubisco LSMT) is disclosed. This enzyme catalyzes methylation of the ε-amine of lysine-14 in the large subunit of Rubisco. In addition, a full-length cDNA clone for Rubisco LSMT is disclosed. Transgenic plants and methods of producing same which (1) have the Rubisco LSMT gene inserted into the DNA, and (2) have the Rubisco LSMT gene product or the action of the gene product deleted from the DNA are also provided. Further, methods of using the gene to selectively deliver desired agents to a plant are …

Forage News [1998-03], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• National Alfalfa Symposium
• Statewide KCA/KFGC Field Day Planned
• Nitrogen for Early Grazing
• Christi Forsythe Receives Award
• Grazing School Update
• Round Bale Silage
• Variety Trial Information
• Contributions of Alfalfa to Wildlife and the Environment
• Planting Date, Fungicide, and Cultivar Effects on Sclerotinia Crown and Stem Rot Severity in Alfalfa
• Forage Quality Variation Among Maize Inbreds: In Vitro Fiber Digestion Kinetics and Prediction with NIRS
• Upcoming Events

Managing Spring Dead Spot Of Bermudagrass, Paul C. Vincelli, David Williams

Agriculture and Natural Resources Publications

Spring dead spot is the most destructive disease of bermudagrass in Kentucky. The most serious outbreaks occur under high maintenance conditions; e.g., high nitrogen fertility, low mowing height, and frequent traffic. Moderate to severe outbreaks can occur under low-maintenance conditions as well.

High levels of resistance to spring dead spot in bermudagrass varieties have not yet been demonstrated experimentally. Limited data suggest that those varieties with best winter hardiness are somewhat less susceptible to the disease. To date, no confirmed cases of spring dead spot have been documented in “Quickstand” bermudagrass, which may indicate that this variety will suffer less …

Manipulation Of High Spatial Resolution Aircraft Remote Sensing Data For Use In Site-Specific Farming, Gabriel B. Senay, Andrew D. Ward, John G. Lyon, Norman R. Fausey, Sue E. Nokes

Biosystems and Agricultural Engineering Faculty Publications

Three spatial data sets consisting of high spatial resolution (1 m) remote sensing images acquired in 12 spectral bands, an on-the-go yield map, and a Digital Elevation Model were co-registered and evaluated for spatial variability studies in a Geographic Information Systems environment. Separate on-the-go yield maps were developed for 3, 5, and 12 statistically significant mean yield classes. For each yield class, the corresponding mean spectral and elevation data were extracted. The relationship between mean spectral and yield data was strongly linear (r = 0.99). Also, a strong linear relationship between mean yield and elevation data (r = 0.92) was …

Forage News [1998-02], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• National Alfalfa Symposium and XVIII Kentucky Alfalfa Conference February 26-27, 1998 University Plaza Hotel & Convention Center Bowling Green, Kentucky
• KFGC Awards to be Presented at Alfalfa Symposium
• U.K. All Commodity Field Day
• Grazing School for Spring 1998
• More Forage Learning Opportunities
• Forage Variety Reports
• Poultry Litter for Pastures
• Influence of Seedling Growth on Flooding Injury in Alfalfa
• Upcoming Events

Forage News [1998-01], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Grazing Conference: Kickoff for KCA
• Kentucky Alfalfa Conference Joins National in 1998
• National Forage Conference in Indianapolis
• Benefits of Rotational Grazing
• Performance of Potato Leafhopper Resistance in Alfalfa During 1997
• Legume Seeding Rates for Renovating Grass Fields
• Upcoming Events

Validating A Vegetative Filter Strip Performance Model, Puneet Srivastava, Thomas A. Costello, Dwayne R. Edwards, James A. Ferguson

Biosystems and Agricultural Engineering Faculty Publications

Vegetative filter strips (VFS) reduce losses of nutrients, solids, and other materials from land area treated with fertilizers and manures. A number of models are available that simulate nutrient and sediment transport in VFS. While VFS effectiveness is considered to depend on lengths of pollutant source area and VFS areas, few published studies have tried to validate these models using variable pollutant source area and VFS area. The objective of this study was to validate an event-based nutrient transport model (Chaubey et al., 1995) that simulates soluble nutrient transport in VFS. This model links three sub-models: modified Green-Ampt infiltration, non-linear …

Fecal Coliform Transport Through Intact Soil Blocks Amended With Poultry Manure, S. W. Mcmurry, Mark S. Coyne, E. Perfect

Plant and Soil Sciences Faculty Publications

Poultry production in Kentucky increased almost 200% between 1991 and 1995. Their waste is typically land applied, and fecal pathogen runoff and infiltration may cause nonpoint source groundwater pollution. We looked at the preferential flow of fecal coliforms through undisturbed soil blocks since fecal bacteria typically infiltrate the soil profile to contaminate groundwater. Poultry manure was uniformly distributed on top of sod-covered or tilled (upper 12.5 cm) soil blocks and the blocks were irrigated. Drainage was collected in 100 uniformly spaced cells beneath each block and analyzed for fecal coliform content and drainage volume. The spatial distribution of drainage and …

Role Of Egr-1 Gene Expression In B Cell Receptor-Induced Apoptosis In An Immature B Cell Lymphoma, Subramanian Muthukkumar, Seong-Su Han, Sumathi Muthukkumar, Vivek M. Rangnekar, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Ligation of B cell receptor (BCR) on BKS-2, an immature B cell lymphoma by anti-IgM antibodies (Ab) caused apoptosis. Here we report that signaling through B cell receptor in wild type BKS-2 cells down-regulated the expression of Egr-1, a zinc finger-containing transcription factor. A reduction in the level ofEgr-1 mRNA could be demonstrated as early as 30 min after the ligation of BCR on BKS-2 cells. Immunocytochemical and Western blot analysis revealed that the expression of EGR-1 protein was also inhibited by anti-IgM treatment. Antisense oligonucleotides to Egr-1 caused growth inhibition and apoptosis in BKS-2 cells, suggesting that …

Effect Of Bmp Implementation On Storm Flow Quality Of Two Northwestern Arkansas Streams, Dwayne R. Edwards, Tommy C. Daniel, H. Don Scott, Phillip A. Moore Jr., John F. Murdoch, Paul F. Vendrell

Biosystems and Agricultural Engineering Faculty Publications

The effectiveness of management practices in improving quality of runoff from agricultural land areas has been reported based primarily on results from plot- and field-scale studies. There is limited information available on watershed scales, particularly when the dominant agricultural land use is pasture. The objective of this study was to determine whether a program of Best Management Practice (BMP) implementation in the Lincoln Lake watershed of northwestern Arkansas was effective in reducing storm stream flow concentrations and mass transport of nitrate nitrogen (NO₃-N), ammonia nitrogen (NH₃-N), total Kjeldahl nitrogen (TKN), ortho-phosphorus (PO₄-P), total phosphorus …

Identification Of A Gal/Galnac Lectin In The Protozoan Hartmannella Vermiformis As A Potential Receptor For Attachment And Invasion By The Legionnaires' Disease Bacterium, Chandrasekar Venkataraman, Bradley J. Haack, Subbarao Bondada, Yousef Abu Kwaik

Microbiology, Immunology, and Molecular Genetics Faculty Publications

The Legionnaire's disease bacterium, Legionella pneumophila, is a facultative intracellular pathogen which invades and replicates within two evolutionarily distant hosts, free-living protozoa and mammalian cells. Invasion and intracellular replication within protozoa are thought to be major factors in the transmission of Legionnaire's disease. Although attachment and invasion of human macrophages by L. pneumophila is mediated in part by the complement receptors CR1 and CR3, the protozoan receptor involved in bacterial attachment and invasion has not been identified. To define the molecular events involved in invasion of protozoa by L. pneumophila, we examined the role of protein tyrosine phosphorylation …

What Leading Alfalfa Producers Do To Produce Quality Hay, Jimmy C. Henning, Garry D. Lacefield, Buddy Sims

Kentucky Alfalfa and Stored Forage Conference

Alfalfa is the highest quality forage legume crop grown in Kentucky and is the number one cash hay crop. Hay raised by Kentucky farmers has been recognized for its quality at local, state (state fair and Kentucky Alfalfa Conference), and national (American Forage and Grassland Council) hay contests. The testing records of the Kentucky Department of Agriculture also document the high quality alfalfa hay of Kentucky. However, little has been written about the farm practices used to produce high quality alfalfa hay in Kentucky. The current practices of top hay growers may be helpful in defining the options available to …

Grazing Alfalfa In Tennessee: Experiences And Opportunities, Gary Bates

Kentucky Alfalfa and Stored Forage Conference

Animal agriculture in the southeastern U.S. is based on forages. This area has the advantage over all other sections of the nation in forage production. From Kentucky to south Florida, with a little planning forage can be grown almost 12 months a year. Forage programs in Tennessee and Kentucky are based primarily around cool-season grasses such as tall fescue and orchardgrass. These grasses grow over a long portion of the year. In fact, producers have to deal with excess forage production during the spring and fall (Fig. 1). The quality and quantity of forage during these times of the year …

Grazing Alfalfa In Lincoln County, Dan Grigson

Kentucky Alfalfa and Stored Forage Conference

Alfalfa grazing is becoming an important practice for our county's large livestock enterprise. Both dairy and beef producers use alfalfa grazing in various parts of their feeding operations. Lincoln County farmers have a reputation for being good forage producers and have over the years produced good yields and good quality alfalfa. Most producers realize that good forage leads to better profits for their livestock enterprises. That's very important to us as the livestock business generates over $21 million in cash receipts annually.

Alfalfa Seed Production In The Western U.S., Garry D. Lacefield

Kentucky Alfalfa and Stored Forage Conference

Alfalfa is the primary forage for the dairy industry in the United States and provides a majority of the nutrients and energy needs for other classes of livestock including beef cattle, horses and sheep.

In order to maximize forage yield, quality and persistence, one should start with high quality seed. Seed must be free of noxious weeds, have a purity higher than 99.5 percent, a germination of 90 percent or higher and true to the variety stated.

Over 80% of the alfalfa seed was grown in the Great Plains and Rocky Mountain states prior to 1948. This was mostly uncertified, …

Do's And Don'ts Of Alfalfa Round Baled Silage, Michael Collins

Kentucky Alfalfa and Stored Forage Conference

Round baled silage provides an option for outside storage of round bales that protects them from weathering. With shorter field exposure times, silage can dramatically increase forage quality compared with hay. Harvest problems caused by wet weather and/or poor drying conditions during spring and for the freeze-down harvest during autumn could be alleviated by incorporating silage into the harvest system.

Alfalfa Hay: Quality Makes The Difference, Garry D. Lacefield, Jimmy C. Henning

Kentucky Alfalfa and Stored Forage Conference

Alfalfa "Queen of the Forage Crops" is one of the most important forage legumes grown in the U.S. It can be grown over a wide range of soil and climatic conditions, it has the highest yield potential and the highest feeding values of all adapted perennial forage legumes. Alfalfa is a versatile crop which can be used for pasture, hay, silage, green-chop, soil improvements and · human consumption (sprouts, etc.).

A Solution To Some Spring-Seeded Alfalfa Problems, Paul C. Vincelli

Kentucky Alfalfa and Stored Forage Conference

One of the most important assignments I received when I assumed this position in 1990 was to address the question, "What is the cause of seeding failure when alfalfa is spring-seeded into soils that remain wet after seeding?" Six years of laboratory and field work have finally borne fruit. Based on our research, we can confidently present Kentucky alfalfa producers with three conclusions and a recommendation.

Manure On Alfalfa: Why, When, And How Much?, Monroe Rasnake

Kentucky Alfalfa and Stored Forage Conference

Manure is an excellent source of nutrients for growing crops. Its' value has been recognized for thousands of years. Before the development of chemical fertilizers, it served as the primary source of nutrients. It has decreased in importance as a nutrient source in this century and in some cases has become a disposal problem. This has led many farmers to consider using it on legume crops such as alfalfa that don't need to have nitrogen added, but will utilize it and other . nutrients in relatively large amounts. The first question to be considered, then, is why apply manure to …

Controlling Weeds In New Seedings And Established Stands, Jonathan D. Green

Kentucky Alfalfa and Stored Forage Conference

The importance of weed control in forage production should not be overlooked, especially when you consider the high investment associated with alfalfa and other legume forages. Weeds may reduce forage yield by competing for water, sunlight and nutrients. For example, yield obtained from the first cutting of alfalfa can be significantly reduced by a heavy infestation of common chickweed. In addition to yield losses, weeds can also lower forage quality, increase the incidence of disease and insect problems, cause premature stand loss, and create harvesting problems. In some cases weeds are unpalatable to livestock or may be poisonous.

What I'Ve Learned From Three Years Of Intensive Experience With No-Till Alfalfa, Jimmy C. Henning, Garry D. Lacefield, Andy Anderson

Kentucky Alfalfa and Stored Forage Conference

Three years ago, a project was begun to better understand the 'hands-on' aspects of no-till establishment of legumes especially alfalfa into fescue sod. Although no-till drills and technology had been around for nearly two decades, farmer feedback on the success of no-till establishment was not uniformly good. The University of Kentucky initiated a program called the 'Establishment Initiative' in partnership with seed companies and Agco-Tye to conduct no-till establishment demonstrations of no-till establishment. These demonstrations focused on getting alfalfa and red clover established into fescue sod which had been suppressed with herbicides. The following are some of the lessons learned.

Foreword [1997], Garry D. Lacefield

Kentucky Alfalfa and Stored Forage Conference

This is the front matter of the proceedings.

Influence Of Topping And Harvest Management On The Evaluation Of Data From Burley Tobacco Variety Trials, Robert C. Pearce, Jim Calvert, Gary K. Palmer

Agronomy Notes

Tobacco producers are always interested in new tobacco varieties, and are continually searching for the "best" variety. Producers receive information about varieties from a number of sources including; research and extension publications, county extension agents, neighbors, farm supply workers, and seed producers. To help producers evaluate varieties, county agents in cooperation with tobacco specialists conduct many burley tobacco variety trials at the county level.

Potassium Soil Test Correlation And Calibration For Burley Tobacco Grown On An Allegheny Loam Soil, Kenneth L. Wells, James E. Dollarhide, Val Shields

Agronomy Notes

Burley tobacco removes large amounts of potassium (K) from soil. A 2,600 pound/A cured leaf crop removes around 200 lbs K/A/yr, with about 110 lbs of that in the leaf and 90 lbs in the stalk. Because of such a heavy soil demand for K, growers are always concerned that application of fertilizer K be sufficient for top production. The University of Kentucky's Soil Testing Laboratory (Division of Regulatory Services) provides a statewide soil testing service. The Mehlich-3 soil extractant is used by the UK lab, and soil test K values from use of this extractant (reported as lbs K/A) …

Effectiveness Of Tile Drainage On A Fragipan Soil In An Orchard Site, Gerald D. Brown, Dwight E. Wolfe, Lloyd W. Murdock

Agronomy Notes

Orchards require a well-drained soil in order for roots to have good aeration and to function properly. The soil is unsatisfactory for orchard purposes if the water table remains within six inches to a foot of the soil surface for a week after a heavy spring rain, or within three feet of the surface for several weeks after growth starts. Poor internal water drainage is a limiting factor for many sites. In Kentucky, many orchards are on soils with a fragipan which result in perched water tables near the surface during winter and spring months. Perched water tables exist above …

Early Maturing Varieties And Soybean Cyst Nematodes: Will This Marriage Work?, Colleen C. Steele, Larry J. Grabau

Agronomy Notes

Soybean cyst nematode (SCN) is widely distributed in Kentucky’s soybean growing areas. The use of SCN-resistant varieties has long been a recommended production practice for infested fields. However, continuous use of such varieties can result in a shift to a race of SCN which is able to vigorously attack previously resistant varieties. For this reason, many states recommend that producers periodically grow a crop of SCN-susceptible soybeans within a crop rotation when SCN populations are at minimal levels (causing less than a 5% loss in yield). The UK Plant Pathology Department recommends a four year rotation in SCN-infested fields [PPA3; …

Nitrogen Source Effects On The Growth And Development Of Burley Tobacco Transplants In The Float System, Robert C. Pearce, Gary K. Palmer

Agronomy Notes

During the spring of 1996, many tobacco producers used a water soluble 20-10-20 fertilizer in their float beds, only to have to throw out the stunted sickly plants that resulted, and start over. The fertilizer that caused the problems turned out to have 100% of the nitrogen (N) as urea-N. It was hypothesized that the poor growth was related to with the conversion of the urea-N to other forms of N. There have been numerous other cases where producers using fertilizers high in urea-N or ammonium-N (NH4-N) have had problems with stunted plant growth. Further study of nitrogen transformations is …