Life Sciences Commons^™

Roundup Ready® Alfalfa, Novel Endophyte Tall Fescue, Red And White Clover, Bermudagrass, And More, S. Ray Smith

Forage Symposium at the Kentucky Cattlemen’s Convention

Finally, Roundup Ready® alfalfa is now available and currently varieties show excellent tolerance to Roundup, good disease res i stance, and good yield potential. Before making plans to plant 100 acres know that the price in most states is over $7.00 per pound and pre-ordering seed is essential if you want to plant the spring of 2006. Roundup tolerance is a very useful trait in alfalfa, but remember that Roundup Ready ® varieties are not superior for other traits. Some current advertisement s promote Roundup Ready® varieties as higher yielding and higher quality. These statements are not untrue, but they …

Using Legumes To Renew Grass Pastures, Garry D. Lacefield

Forage Symposium at the Kentucky Cattlemen’s Convention

Legume content in Kentucky pastures has been reduced dramatically as a result of the drought and high temperatures of 2005 and very muddy conditions this past winter. In addition, late-winter early-spring seedings during 2004 were not as successful as normal due to excessive moisture during the establishment periods. As a result of our reduced amount of legumes in pasture and the many advantages legumes have for improving Kentucky pastures, serious considerations should be given to renovating pastures in 2006.

Foreword [2006], Garry D. Lacefield, Christi L. Forsythe

Forage Symposium at the Kentucky Cattlemen’s Convention

No abstract provided.

Forage News [2006-01], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Forages at KCA
• Heart of America Grazing Conference
• 26th Kentucky Alfalfa Conference
• Selecting Varieties for Kentucky
• Upcoming Events

Nitrapyrin (N-Serve) With Anhydrous Ammonia At/Near Corn Planting, John H. Grove

Agronomy Notes

Higher prices for energy are driving corn fertilizer nitrogen (N) prices higher as well. This increases interest in alternative management practices and products that optimize corn’s N nutrition, but maintain grower profit. Alternatives permitting fertilizer N rate reductions include: a) changes in N application timing and placement; b) using alternative sources of N (ex. poultry litter) to meet part of corn’s N need; and c) using fertilizer N additives that improve N use efficiency by inhibiting one or more N loss processes in the soil N cycle (biological N transformations).

Vertical Loads Due To Wheat On Obstructions Located On The Floor Of A Model Bin, Marek Molenda, Michael D. Montross, Sidney A. Thompson, Jozef Horabik

Biosystems and Agricultural Engineering Faculty Publications

Tests were conducted in a model grain bin to evaluate the vertical loads acting on differently shaped obstructions embedded in wheat during filling, detention, and discharge. The bin had corrugated galvanized steel walls with a 1.83 m diameter and a flat bottom. All tests were conducted in a bin that was centrically loaded and unloaded. Three differently shaped obstructions (disc, cone, and cylinder) were tested; each had a circular base equivalent to 6% of the bin floor area. The obstructions were supported in the bin using a three-legged support structure. Each leg of the support structure rested on a load …

The Kentucky Agricultural Economic Outlook For 2006, Linda Inman, A. Lee Meyer, Gina Wilkie

Kentucky Agricultural Economic Outlook

No abstract provided.

Kentucky Water Resources Research Institute Annual Technical Report Fy 2005, Kentucky Water Resources Research Institute, University Of Kentucky

KWRRI Annual Technical Reports (USGS’s 104b Grant Program)

The 2005 Annual Technical Report for Kentucky consolidates reporting requirements of the Section 104(b) base grant award in a single technical report that includes: 1) a synopsis of each research project supported during the period, 2) related reports and publications, 3) a description of information transfer activities, 4) a summary of student support during the reporting period, and 5) notable achievements and awards during the year.

Nlm Database Central: The First Place To Look For Your Phsr Research Data, F. Douglas Scutchfield Md

Preventive Medicine and Environmental Health Presentations

No abstract provided.

Localization Of Diplodia Pinea In Diseased And Latently-Infected Pinus Nigra, Jennifer Lee Flowers

University of Kentucky Doctoral Dissertations

Diplodia pinea causes Diplodia tip blight on more than 30 different pine species. During the past 10 years, Diplodia tip blight has emerged as a serious problem in landscape and Christmas tree farms in this region. Surveys of diseased and symptomless Austrian pines revealed that latent infections of symptomless shoots by D. pinea were common. Latent infections may account for the recently observed rapid decline of mildly diseased pines in our region. To investigate the colonization habits of D. pinea within its host, molecular cytology was attempted and traditional histology was performed on naturally infected, diseased and asymptomatic Austrian pine …

The Speciation Of Metals In Mammals Influences Their Toxicokinetics And Toxicodynamics And Therefore Human Health Risk Assessment, Robert A. Yokel, Stephen M. Lasley, David C. Dorman

Pharmaceutical Sciences Faculty Publications

Chemical form (i.e., species) can influence metal toxicokinetics and toxicodynamics and should be considered to improve human health risk assessment. Factors that influence metal speciation (and examples) include: (1) carrier-mediated processes for specific metal species (arsenic, chromium, lead and manganese), (2) valence state (arsenic, chromium, manganese and mercury), (3) particle size (lead and manganese), (4) the nature of metal binding ligands (aluminum, arsenic, chromium, lead, and manganese), (5) whether the metal is an organic versus inorganic species (arsenic, lead, and mercury), and (6) biotransformation of metal species (aluminum, arsenic, chromium, lead, manganese and mercury). The influence of speciation on metal …

Air Exchange Rate In A Horse Trailer During Road Transport, Joseph L. Purswell, Richard S. Gates, Laurie M. Lawrence, Jamey D. Jacob, Timothy S. Stombaugh, Robert J. Coleman

Biosystems and Agricultural Engineering Faculty Publications

Horses traveling by road commonly experience heat stress conditions and poor air quality, which may be caused by insufficient ventilation; however, there are few estimates of air exchange in a horse trailer during transport. Air exchange rate was measured at ten locations within a four-horse trailer (internal volume 18.5 m³) using tracer gas decay measurement to assess the adequacy of ventilation. Three vehicle speeds (13, 48, and 97 km h^-1) and three window configurations (all windows and roof vents closed, all windows open, all windows open and roof vents open forward) were tested with and without …

Influences Of Host Size And Host Quality On Host Use In A Seed-Feeding Beetle, Angela Rocío Amarillo-Suárez

University of Kentucky Doctoral Dissertations

For insects that develop inside discrete hosts both host size and host quality constrain offspring growth, influencing the evolution of body size and life history traits. This dissertation examines the effects of host size, host quality, and intraspecific competition on life history and associated traits of populations of the seed-feeding beetle S. limbatus adapted to different host plants, and quantifies population differences in phenotypic plasticity. Populations of the study correspond to divergent clades of the species phylogeography (Colombia and United States).

Clades compared differ genetically for all traits when beetles were raised in a common garden. Contrary to expectations from …

Forage News [2005-12], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Grazing Conference in Cave City
• Renew KFGC Membership
• National Forage Meetings
• Still Room for Improvement
• Growth Performance by Fall-Calving Cows Grazing Tall Fescue Pastures with Different Proportions Stockpiled Until Late Fall
• Effect of Maturity on Quality of Various Small Grains
• Effect of N Prices on Fertilization of Cool-Season Grasses
• Value and Amount of Nitrogen Fixed by Various Legumes
• Reflections: 2005
• Upcoming Events

Sal-Site: Integrating New And Existing Ambystomatid Salamander Research And Informational Resources, Jeramiah J. Smith, Srikrishna Putta, John A. Walker, D. Kevin Kump, Amy K. Samuels, James R. Monaghan, David W. Weisrock, Chuck Staben, S. Randal Voss

Biology Faculty Publications

Salamanders of the genus Ambystoma are a unique model organism system because they enable natural history and biomedical research in the laboratory or field. We developed Sal-Site to integrate new and existing ambystomatid salamander research resources in support of this model system. Sal-Site hosts six important resources: 1) Salamander Genome Project: an information-based web-site describing progress in genome resource development, 2) Ambystoma EST Database: a database of manually edited and analyzed contigs assembled from ESTs that were collected from A. tigrinum tigrinum and A. mexicanum, 3) Ambystoma Gene Collection: a database containing full-length protein-coding sequences, 4) Ambystoma Map and Marker …

Forage News [2005-11], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• KFGC Awards: Nominations Due by December 14
• Forages at KCA
• Program Set for Grazing Conference
• Alfalfa Conference to be in Lexington
• How Sensitive is Alfalfa to Low Soil pH?
• Fertilizer Value in Hay
• Prussic Acid and Nitrate Poisoning Are Not the Same
• Hay May be Tight
• Consider Grazing Crop Residue
• Ever Been to a Hay Auction………?
• Upcoming Events

Distribution Pattern Variability Of Granular Vrt Applicators, John P. Fulton, Scott A. Shearer, Stephen F. Higgins, Dennis Wayne Hancock, Timothy S. Stombaugh

Biosystems and Agricultural Engineering Faculty Publications

Granular applicators equipped with variable-rate technology (VRT) have gained popularity in recent years as a result of increased interest in variable-rate application. The purpose of this investigation was to characterize distribution patterns at varying rates for different granular applicators. Uniform-rate (UR) tests were conducted to assess the accuracy of variable-rate application from four granular applicators: two spinner-disc spreaders (A and B), and two pneumatic applicators (C and D). Pattern results indicated a consistent triangular pattern for spinner spreader B and consistent patterns for the pneumatic applicators (C and D). However, applicator D produced pattern variations at the center and right …

Rate Response Assessment From Various Granular Vrt Applicators, John P. Fulton, Scott A. Shearer, Stephen F. Higgins, Matthew J. Darr, Timothy S. Stombaugh

Biosystems and Agricultural Engineering Faculty Publications

Variable-rate technology (VRT) adds complexity to application equipment, thereby confounding the assessment of applicator performance. The intent of this investigation was to assess the rate response of various VRT granular applicators: two spinner spreaders (A and B), and two pneumatic applicators (C and D). Variable-rate (VR) tests were conducted to quantify the rate response characteristics (delay and transition times) for the applicators. A sigmoidal function was used to model the rate response for five of the six tests. Applicator A exhibited a linear response during decreasing rate changes. Results indicated that only applicator B demonstrated consistent delay and transition times, …

Forage News [2005-10], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Kentucky Agricultural Income Tops Four Billion Dollars
• New Tall Fescue Book
• Alfalfa Hay for Horses: Myths and Realities
• Building Hay Storage Structures with Agriculture Development Board’s Cost Share Program
• KFGC Fall Board Meeting
• AFGC Annual Meeting - San Antonio March 10-14
• Late Fall Fertilization of Cool Season Grasses
• Prussic Acid Poisoning
• Comparative Lamb and Heifer Growth Rates on Non-Toxic and Endophyte-Free Tall Fescue
• Upcoming Events

Agricultural Situation And Outlook Fall 2005, Matt Ernst, Kenneth H. Burdine

Agricultural Situation and Outlook

No abstract provided.

Forage News [2005-09], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Scott County to Host Fall Grazing School
• Now is the Time to Test
• Economics of Grazing Stocker Cattle as a Sustainable Alternative to Row Crops
• Roundup Ready Alfalfa
• Novel Endophyte Tall Fescue
• Is It Too Late to Stockpile This Year?
• KFGC Awards
• KFGC Executive Committee to Meet September 6
• Wisconsin Grazing Dairy Farms
• Upcoming Events

Cpg Oligodeoxynucleotides Stimulate Cord Blood Mononuclear Cells To Produce Immunoglobulins., Cheri D Landers, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

CpG oligodeoxynucleotides (ODNs) stimulate adult B cells leading to cellular proliferation and immunoglobulin production. It is unknown if CpG-ODNs similarly stimulate neonatal human B cells. Neonates have immature immune responses and are poorly responsive to thymus independent antigens such as polysaccharides. We determined umbilical cord cells' response to CpG-ODNs. Adult and umbilical cord B cells produced similar amounts of IgM (adult 1371 +/- 352 vs. cord 1873 +/- 1084 ng/ml) in response to CpG-ODN stimulation. Although CpG-ODN was able to stimulate adult IgG and IgA production, cord cells produced less IgG (153 +/- 58 vs. 10 +/- 2.5 ng/ml) and …

Effects Of Commercial Diazinon And Imidacloprid On Microbial Urease Activity In Soil And Sod, C. W. Ingram, Mark S. Coyne, David W. Williams

Plant and Soil Sciences Faculty Publications

Diazinon [O,O-diethyl O-2-isopropyl-6-methyl(pyrimidine-4-yl) phosphorothioate] and imidacloprid [1-(1-[6-chloro-3-pyridinyl]methyl)-N-nitro-2-imidazolidinimine] are applied to lawns for insect control simultaneously with nitrogenous fertilizers such as urea, but their potential effect on urease activity and nitrogen availability in turfgrass management has not been evaluated. Urease activity in enzyme assays, washed cell assays, and soil slurries was examined as a function of insecticide concentration. Intact cores from field sites were used to assess the effect of insecticide application on urease activity in creeping bentgrass (Agrostis palustris Huds.) and bluegrass (Poa pratensis L.) sod. Bacterial urease fromBacillus pasteurii and plant …

Effects Of Dietary Curcumin Or N-Acetylcysteine On Nf-Kappab Activity And Contractile Performance In Ambulatory And Unloaded Murine Soleus, Mehran Farid, Michael B. Reid, Yi-Ping Li, Eric Gerken, William J. Durham

Physiology Faculty Publications

BACKGROUND: Unloading of skeletal muscle causes atrophy and loss of contractile function. In part, this response is believed to be mediated by the transcription factor nuclear factor-kappa B (NF-kappaB). Both curcumin, a component of the spice turmeric, and N-acetylcysteine (NAC), an antioxidant, inhibit activation of NF-kappaB by inflammatory stimuli, albeit by different mechanisms. In the present study, we tested the hypothesis that dietary curcumin or NAC supplementation would inhibit unloading-induced NF-kappaB activity in skeletal muscle and thereby protect muscles against loss of mass and function caused by prolonged unloading.

METHODS: We used hindlimb suspension to unload the hindlimb muscles of …

Fatigue Resistance Of Rat Extraocular Muscles Does Not Depend On Creatine Kinase Activity, Colleen A. Mcmullen, Katrin Hayeß, Francisco H. Andrade

Physiology Faculty Publications

BACKGROUND: Creatine kinase (CK) links phosphocreatine, an energy storage system, to cellular ATPases. CK activity serves as a temporal and spatial buffer for ATP content, particularly in fast-twitch skeletal muscles. The extraocular muscles are notoriously fast and active, suggesting the need for efficient ATP buffering. This study tested the hypotheses that (1) CK isoform expression and activity in rat extraocular muscles would be higher, and (2) the resistance of these muscles to fatigue would depend on CK activity.

RESULTS: We found that mRNA and protein levels for cytosolic and mitochondrial CK isoforms were lower in the extraocular muscles than in …

Composition And Methods Of Using The Mirabilis Mosaic Caulimovirus Sub-Genomic Transcript (Sgt) Promoter For Plant Genetic Engineering, Indu B. Maiti, Nrisingha Dey

Kentucky Tobacco Research and Development Center Faculty Patents

The isolation of and methods of using a sub-genomic transcript (Sgt) promoter from Mirabilis mosaic virus (MMV) are described. A 333 bp MMV Sgt promoter fragment (sequence−306 to +27 from the transcription start site, TSS) was found to be sufficient for strongest promoter activity. This MMV Sgt promoter fragment shows comparable promoter activity to the MMV FLt promoter both in transgenic plants and in protoplasts. The MMV Sgt promoter also demonstrates much greater activity compared to Cauliflower mosaic virus (CaMV) 19S promoter and 35S promoter. The MMV Sgt promoter fragment and any chimeric gene to which it may be linked …

C-Jun N-Terminal Kinase (Jnk) Is Required For Survival And Proliferation Of B-Lymphoma Cells, Murali Gururajan, Roger Chui, Anbu K. Karuppannan, Jiyuan Ke, C. Darrell Jennings, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Several primary murine and human B lymphomas and cell lines were found to constitutively express high levels of the activated form of c-jun N-terminal kinase (JNK), a member of the mitogen-activated protein (MAP) kinase family. Proliferation of murine B lymphomas CH31, CH12.Lx, BKS-2, and WEHI-231 and the human B lymphomas BJAB, RAMOS, RAJI, OCI-Ly7, and OCI-Ly10 was strongly inhibited by SP600125, an anthrapyrazolone inhibitor of JNK, in a dose-dependent manner. The lymphoma cells underwent apoptosis and arrested at the G2/M phase of cell cycle. Furthermore, JNK-specific small interfering RNA (siRNA) inhibited the growth of both murine and human B lymphomas. …

Comparative Genetic Studies Of Native And Introduced Coccinellidae In North America, Elliot S. Krafsur, John J. Obrycki, James D. Harwood

Entomology Faculty Publications

During the past four decades, several species of aphidophagous Coccinellidae became established in North America, including Coccinella septempunctata, Harmonia axyridis, Hippodamia variegata, and Propylea quatuordecimpunctata. After their establishment, unknown circumstances favoured a rapid increase in population densities and distribution of H. axyridis and C. septempunctata at localities hundreds and thousands of kilometers from their release sites. Propylea quatuordecimpunctata and Hippodamia variegata have spread more slowly after becoming established in northeastern North America. Comparative studies based upon allozyme variation in these four introduced species and in six native North American species of ladybird beetles revealed …

Quantifying Aphid Predation Rates Of Generalist Predators In The Field, James D. Harwood, John J. Obrycki

Entomology Faculty Publications

The community of predators within agroecosystems has the potential to restrict aphid populations, especially early in the season before exponential increases in density and prior to the arrival of specialist natural enemies. Although direct observations of predation, laboratory feeding trials and manipulative field studies have been used to estimate levels of biological control exerted by different species (or potentially negative interactions between them), it is often difficult to extrapolate results to naturally occurring interactions in the field.

Over 100 investigations have utilized gut-content analysis to estimate aphid predation rates by predators. Throughout the last century, gut dissection, which enables the …

Runx-Dependent Expression Of Pkc Is Critical For Cell Survival In The Sea Urchin Embryo, Carrie Dickey-Sims, Anthony J. Robertson, Dawn E. Rupp, John J. Mccarthy, James A. Coffman

Physiology Faculty Publications

BACKGROUND: Runx transcription factors play critical roles in the developmental control of cell fate and contribute variously as oncoproteins and tumor suppressors to leukemia and other cancers. To discover fundamental Runx functions in the cell biology of animal development, we have employed morpholino antisense-mediated knockdown of the sea urchin Runx protein SpRunt-1. Previously we showed that embryos depleted of SpRunt-1 arrest development at early gastrula stage and underexpress the conventional protein kinase C SpPKC1.

RESULTS: We report here that SpRunt-1 deficiency leads to ectopic cell proliferation and extensive apoptosis. Suppression of the apoptosis by pharmacological inhibition of caspase-3 prevents the …