Life Sciences Commons^™

Soft Red Winter Wheat Elite Germplasm Screening And Evaluation For Stripe Rust In The Us Southeast Region, Ehsan Shakiba, Noah Dewitt, Ali Babar, Richard E. Boyles, Mohamed Mergoum, Russell Sutton, Jeanette Lyerly, Stephen Harrison

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Stripe rust is a severe disease affecting wheat (Triticum aestivum L.) production in the United States Southeast region, necessitating the identification of resistant sources. The study was conducted at the University of Arkansas, Fayetteville, and utilized a Randomized Block Design over three years. A total of 1130 lines were inoculated annually, and their responses for stripe rust were recorded. The results revealed that 11%, 79%, and 77% of the lines showed resistance in the first, second, and third years, respectively, with an overall 50% of the whole population. Linear Mixed Model and Generalized Estimating Equation analyses highlighted environmental influences, …

Electron-Beam-Killed Staphylococcus Vaccine Reduced Lameness In Broiler Chickens, Anna L.F.V. Assumpcao, Komala Arsi, Andi Asnayanti, Khawla Alharbi, Anh Dang Trieu Do, Quentin D. Read, Ruvindu Perera, Abdulkarim Shwani, Amer Abdullah Hasan, Suresh D. Pillai, Robin C. Anderson, Annie M. Donoghue, Douglas D. Rhoads, Palmy R.R. Jesudhasan, Adnan A. Alrubaye

Poultry Science Faculty Publications and Presentations

Broiler chicken lameness caused by bacterial chondronecrosis with osteomyelitis (BCO) is presently amongst the most important economic and animal welfare issues faced by the poultry industry, and the estimated economic loss is around USD 150 million. BCO lameness is associated with multiple opportunistic bacterial pathogens inhabiting the respiratory and gastrointestinal tracts. In cases of immune deficiency resulting from stress, injury, or inflammation of the tissue, opportunistic pathogens, mainly Staphylococcus spp., can infiltrate the respiratory or gastrointestinal mucosa and migrate through the bloodstream to eventually colonize the growth plates of long bones, causing necrosis that leads to lameness. This is the …

A Comparison Of Inversion Test Holding Methods For Broilers, A. Perretti, S. Weimer

Poultry Science Faculty Publications and Presentations

Objective, robust, and repeatable assessments of fear responses of poultry can lead to improvements in research techniques, the validity of test results, and ultimately bird welfare. The objective of this study was to examine the effect of 2 different holding methods on broiler fear responses during the inversion test, a standardized method of assessing fear in poultry. On D15 and D34 mixed-sex broilers (N = 80) were inverted and held either by their shanks (N = 40) or feet (N = 40) at arm's length away from the experimenter for 30 s. The frequency of wing flapping, head movement, attempts …

Blackberry Growth Monitoring And Feature Quantification With Unmanned Aerial Vehicle (Uav) Remote Sensing, Akwasi Tagoe, Alexander Silva, Cengiz Koparan, Aurelie Poncet, Dongyi Wang, Donald Johnson, Margaret Worthington

Biological and Agricultural Engineering Faculty Publications and Presentations

Efficiently managing agricultural systems necessitates accurate data collection from crops to examine phenotypic characteristics and improve productivity. Traditional data collection processes for specialty horticultural crops are often subjective, labor-intensive, and may not provide accurate information for precise management decisions in phenotypic studies and crop production. Reliable and standardized techniques to record and evaluate crop features using agricultural technology are essential for improving agricultural systems. The objective of the research was to develop a methodology for accurate measurement of blackberry flowers and vegetation coverage using UAV remote sensing and image analysis. The UAV captured 20,812 images in the visible spectrum, and …

Structural Projections To The Nucleus Accumbens Link To Impulsive Components Of Human Risk Preference, Loreen Tisdall, Kelly H. Macniven, Josiah Leong, Renato Frey, Jörg Rieskamp, Ralph Hertwig, Brian Knutson, Rui Mata

Psychological Science Faculty Publications and Presentations

Functional responses in the Nucleus Accumbens (NAcc) to risk- and reward-related cues can predict real-life risk-taking behavior. Since NAcc activity depends on neurotransmission from connected brain regions, projections to the NAcc may also predict risk preference. To quantify risk preference, we employed latent variables previously derived in a comprehensive, independent study examining the psychometric structure of risk preference, which yielded a general risk preference factor as well as several specific factors, including a factor capturing impulsivity. Informed by previous work, we preregistered a set of hypotheses concerning the association between different risk preference factors and fractional anisotropy (or FA, which …

Natural Panola Mountain Ehrlichia Infections In Cattle In A Longitudinal Study Of Angus Beef Calves, Cierra Briggs, Drew Casey, Anna Grace Deakins, Jeremy G. Powell, Kelly Loftin, Emily G. Mcdermott

Entomology and Plant Pathology Faculty Publications and Presentations

Panola Mountain Ehrlichia (PME) is an emerging zoonotic pathogen, transmitted by Amblyomma americanum ticks in the southeastern United States. It is closely related to Ehrlichia ruminantium, the causative agent of heartwater disease. Heartwater disease is an often-fatal illness of ruminant livestock present in Africa and the Caribbean. The taxonomic relationship between PME and E. ruminantium has raised concerns about the pathogenicity of PME in livestock. To determine whether cattle could be naturally infected with PME in an endemic area, we conducted a one-year longitudinal study of Angus-breed beef calves in Fayetteville, Arkansas. One hundred seventy-seven calves born between September …

Physiochemical, Bio, Thermal, And Non-Thermal Processing Of Major And Minor Millets: A Comprehensive Review On Antinutritional And Antioxidant Properties, Suhan Bheemaiah Balyatanda, N.A. Nanje Gowda, Jeyamkondan Subbiah, Snehasis Chakraborty, P.V. Vara Prasad, Kaliramesh Siliveru

Food Science Faculty Publications and Presentations

Millets are recognized as future foods due to their abundant nutrition and resilience, increasing their value on the global stage. Millets possess a broad spectrum of nutrients, antinutrients, and antioxidants, making it imperative to understand the effects of various processing methods on these components. Antinutritional factors interfere with the digestibility of macro-nutrients and the bioavailability and bio accessibility of minerals. This necessitates methods to reduce or eliminate antinutrients while improving nutritive and antioxidant value in food. This review aims to elucidate the rationale behind processing choices by evaluating the scientific literature and examining the mechanisms of processing methods, categorized as …

The Divergent Responses Of Salinity Generalists To Hyposaline Stress Provide Insights Into The Colonisation Of Freshwaters By Diatoms, Kathryn J. Judy, Eveline Pinseel, Kala M. Downey, Jeffrey A. Lewis, Andrew J. Alverson

Biological Sciences Faculty Publications and Presentations

Environmental transitions, such as the salinity divide separating marine and fresh waters, shape biodiversity over both shallow and deep timescales, opening up new niches and creating opportunities for accelerated speciation and adaptive radiation. Understanding the genetics of environmental adaptation is central to understanding how organisms colonise and subsequently diversify in new habitats. We used time-resolved transcriptomics to contrast the hyposalinity stress responses of two diatoms. Skeletonema marinoi has deep marine ancestry but has recently invaded brackish waters. Cyclotella cryptica has deep freshwater ancestry and can withstand a much broader salinity range. Skeletonema marinoi is less adept at mitigating even mild …

Integrating Ecophysiology And Omics To Unlock Crop Response To Drought And Herbivory Stress, Insha Shafi, Manish Gautam, Rupesh Kariyat

Entomology and Plant Pathology Faculty Publications and Presentations

This article is part of the Research Topic: Plant Ecophysiology: Responses to Climate Changes and Stress Conditions

Noncontact Diffuse Reflectance Spectroscopy Of Synovial Fluid Samples For Rapid Identification Of Infections, Erin E. Drewke, Robert L. Brand, Caroline G. Geels, Hanna K. Jensen, Kevin Wong, Jarret D. Sanders, Narasimhan Rajaram

Biomedical Engineering Faculty Publications and Presentations

Severe joint infections, such as septic arthritis, require rapid diagnostic testing of the synovial fluid aspirated from joints level so that a surgical team can be assembled quickly. We present a diffuse reflectance spectroscopy (DRS) system for noncontact determination of infection. Using a light-tight syringe holder and fiber optic probe, diffusely reflected light from 475 to 655 nm was acquired from 18 patient samples through the wall of a syringe in a noncontact and sterile manner. We determined the reflectance ratios at two different wavelengths—R ₄₉₀/R ₆₀₀ and R ₅₈₀/R ₆₀₀ and found statistically significant …

Advancing Crispr-Based Solutions For Covid-19 Diagnosis And Therapeutics, Roaa Hadi, Abhishek Poddar, Shivakuma Sonnaila, Venkata Suryanarayana Murthy Bhavaraju, Shilpi Agrawal

Chemistry & Biochemistry Faculty Publications and Presentations

Since the onset of the COVID-19 pandemic, a variety of diagnostic approaches, including RT-qPCR, RAPID, and LFA, have been adopted, with RT-qPCR emerging as the gold standard. However, a significant challenge in COVID-19 diagnostics is the wide range of symptoms presented by patients, necessitating early and accurate diagnosis for effective management. Although RT-qPCR is a precise molecular technique, it is not immune to false-negative results. In contrast, CRISPR-based detection methods for SARS-CoV-2 offer several advantages: they are cost-effective, time-efficient, highly sensitive, and specific, and they do not require sophisticated instruments. These methods also show promise for scalability, enabling diagnostic tests. …

Structural Molecular And Developmental Evidence For Cell-Type Diversity In Cnidarian Mechanosensory Neurons, Julia Baranyk, Kristen Malir, Miguel A.P. Silva, Sakura Rieck, Gracie Scheve, Nagayasu Nakanishi

Biological Sciences Faculty Publications and Presentations

Deploying a conserved mechanosensory neuron known as the concentric hair cell, cnidarians have evolved diverse mechanoreceptors from hydroid filiform tentacles to jellyfish statocysts. However, it is unknown whether cnidarian mechanoreceptor evolution has relied solely on repurposing a single ancestral mechanosensory neuron type. Here we report evidence for cell-type diversity of mechanosensory neurons in sea-anemone cnidarian Nematostella vectensis. Uncovered in the ectoderm of feeding tentacles are conventional type I hair cells and previously unrecognized type II hair cells differing in the structure of apical sensory apparatus and synapses. Moreover, we identify TRP channel-encoding gene polycystin-1 as a type-II-hair-cell-specific essential mediator of …

Ad Fontes: Divergence-Time Estimation And The Age Of Angiosperms, Stephen A. Smith, Jeremy M. Beaulieu

Biological Sciences Faculty Publications and Presentations

Accurate divergence times are essential for interpreting and understanding the context in which lineages have evolved. Over the past several decades, debates have surrounded the discrepancies between the inferred molecular ages of crown angiosperms, often estimated from the Late Jurassic into the Permian, and the fossil record, placing angiosperms in the Early Cretaceous. That crown angiosperms could have emerged as early as the Permian or even the Triassic would have major implications for the paleoecological context of the origin of one of the most consequential clades in the tree of life. Here, we argue, and demonstrate through simulations, that the …

Economic Considerations Of In-Season Potassium Applications To Soybean Using Payoff Matrices, Carrie Ortel, T. L. Roberts, M. Popp, W. J. Ross, N. A. Slaton, M. R. Parvej

Agricultural Economics and Agribusiness Faculty Publications and Presentations

Potassium (K) deficiency is a common yield-limiting factor in Arkansas soybean (Glycine max (L.) Merr.) production that can be addressed with innovative supplemental fertilizer application. An established leaf sampling protocol and dynamic critical concentration allow accurate diagnosis of K deficiency with corresponding recommendations for corrective, in-season K fertilizer applications at site-specific rates and times to reach anticipated yield goals. However, the profitability of in-season K fertilizer applications to irrigated soybean remains unclear. Research was conducted in Arkansas from 2021 to 2023 to evaluate multiple rates of in-season applications of muriate of potash to soybean at 15 and 30 days …

Assessing Soybean Cultivar Resistance To Target Spot Using A Detached Leaf Assay, Rachel Wolf, Robert Holland, J. Alejandro Rojas, Samuel B. Fernandes, J. C. Rupe

Entomology and Plant Pathology Faculty Publications and Presentations

Target spot (TS), caused by the fungus Corynespora cassiicola, is a foliar disease
of increasing importance to soybean in the United States. Identifying cultivar re-
sistance to TS is challenging. The objective of this research was to determine the
reactions of a diverse collection of soybean genotypes to TS using a detached
leaf inoculation method. A detached unifoliate leaf of each soybean genotype
was placed in a Petri dish on moist filter paper, wounded at three locations, inoc-
ulated with 10 μl of a 10 5 -conidial suspension of C. cassiicola, and incubated at
room temperature (23°C). The percent leaf …

Onion Peel Extract Prevents Intestinal Inflammation Via Amk-Activated Protein Kinase Activation In Caco-2/Ht-29 Cells, Olugbenga Balogun, Cindi Brownmiller, Sun-Ok Lee, Hye Won Kong

Food Science Faculty Publications and Presentations

Background: Obesogenic diets cause intestinal inflammation and dysfunction. Polyphenols have shown a positive impact on reducing inflammation in in vitro studies. However, their bioactivity may not be the same in the in vivo system due to structural alteration by the gastrointestinal digestive process. The purpose of this study was to investigate the anti-inflammatory effect of onion peel and its major bioactive compound, quercetin, in the intestine and further examine the impact of intestinal digestion on this effect. Methods: Onion peel extract (OPE) and quercetin (Q) were digested using gastrointestinal digestive enzymes in vitro and then treated into lipopolysaccharide (LPS)-stimulated Caco-2/HT-29 …

Shade, Height Of Cut, And Plant Growth Regulator Effects On Bermudagrass And Zoysiagrass Putting Greens, Thomas E. Walton, John Mccalla, Douglas E. Karcher, W. J. Hutchens, Ambika Chandra, Michael D. Richardson

Horticulture Faculty Publications and Presentations

Warm-season turfgrasses, especially hybrid bermudagrass (Cynodon dactylon (L.) Pers × Cynodon transvaalensis Burtt-Davy), continue to be widely used on golf course putting greens in tropical and subtropical climates globally and are also gaining popularity in the transition zone region of the United States. Hybrid bermudagrass produces a high-quality putting green surface; however, it has relatively poor shade and cold tolerance. Recently, fine-textured zoysiagrass (Zoysia spp.) cultivars have been released and may be another potential option for golf course putting greens, especially since zoysiagrass is generally considered more shade tolerant than bermudagrass. “Lazer (DALZ 1308)” zoysiagrass (Zoysia …

Conformational Cycle Of A Protease-Containing Abc Transporter In Lipid Nanodiscs Reveals The Mechanism Of Cargo-Protein Coupling, Ruojiing Zhang, Kevin L. Jagessar, University Of Arkansas, Fayetteville, Adithya Polasa, Richard A. Stein, Mahmoud Moradi, Erkan Karakas, Hassane S. Mchaourab

Chemistry & Biochemistry Faculty Publications and Presentations

Protease-containing ABC transporters (PCATs) couple the energy of ATP hydrolysis to the processing and export of diverse cargo proteins across cell membranes to mediate antimicrobial resistance and quorum sensing. Here, we combine biochemical analysis, single particle cryoEM, and DEER spectroscopy in lipid bilayers along with computational analysis to illuminate the structural and energetic underpinnings of coupled cargo protein export. Our integrated investigation uncovers competitive interplay between nucleotides and cargo protein binding that ensures the latter’s orderly processing and subsequent transport. The energetics of cryoEM structures in lipid bilayers are congruent with the inferred mechanism from ATP turnover analysis and reveal …

Shell Translucency: A Novel Eggshell Quality Parameter In Broiler Breeders, Duarte Neves, Austin Jasek, Colwayne Morris, Raquel Burin, Kevin Stephens, Marco Rebollo

Proceedings of the Arkansas Nutrition Conference

In the United States, nearly 240 million commercial broiler breeder eggs are incubated weekly with an average hatchability of 79.1% (Third Party Reporting). Between 160 eggs/hen and 125 chicks/hen the loss is considerable. In 2007, an estimated 1% improvement in hatchability would escalate profits by over $25 million to hatcheries and markedly increase profitability in broiler operations (Schaal and Gherian, 2007). In order to tap into top hatchability performances, egg management in commercial hatcheries such as egg storage time and conditions, incubation temperature and humidity, as well as turning frequency are of utmost importance (Vick et al., 1993; Reis et …

A Receptor For Dual Ligands Governs Plant Immunity And Hormone Response And Is Targeted By A Nematode Effector, Li Huang, Yulin Yuan, Chloe Ramirez, Zhe Zhao, Letien Chen, Thomas Griebl, Joanna Kud, Joseph C. Kuhl, Allan Caplan, Louise-Marie Dandurand, Fangming Xiao

Entomology and Plant Pathology Faculty Publications and Presentations

Significance

Abstract

Strategic Light Use Efficiency Optimization Of Hydroponic Lettuce Exposed To Different Photosynthetic Photon Flux Densities, Peyton Lou Palsha, Marc W, Van Iersel, Ryan W. Dickson, Lynne Seymour, Kuan Qin, Rhuanito Saranz Ferrarezi

Horticulture Faculty Publications and Presentations

Light use efficiency characterizes the ability of a crop to convert radiation into biomass. Determining optimum cultivar-specific photosynthetic photon flux density (PPFD) values from sole-source lighting can be used to optimize leaf expansion, maximize biomass, and shorten the production period. This study evaluated the growth of hydroponic lettuce (Lactuca sativa) ‘Rex’ cultivated under different PPFD levels using sole-source lighting. At lower PPFD levels of 201 to 292 µmol·m⁻²·s⁻¹, the plant projected canopy size (PCS) and specific leaf area increased to enhance light capture by 36.2% as compared to higher PPFD levels (333 and 413 µmol·m^{−2 …}

Minimizing Risks Of Off-Target Movement Of Florpyrauxifen-Benzyl By Coating Onto Urea, Bodie L. Cotter, Jason K. Norsworthy, Thomas R. Butts, Trenton L. Roberts, Andy Mauromostakos

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Commercialization of florpyrauxifen-benzyl as Loyant^® in 2018 as a synthetic auxin herbicide in rice was followed by soybean injury due to off-target movement of spray applications in the mid-southern United States. Concerns surrounding off-target movement led to the exploration of an alternative application method to help alleviate the issue. Field experiments were conducted in 2020 and 2021 to explore the likelihood of a reduction in soybean injury following applications of florpyrauxifen-benzyl coated on urea in narrow- and wide-row soybean systems and to determine the likelihood of volatilization from this novel application method. Florpyrauxifen-benzyl spray-applied at 0.18 g ai ha …

Resistance To Acetyl Coenzyme A Carboxylase (Accase) Inhibitor In Lolium Multiflorum: Effect Of Multiple Target-Site Mutations, Gulab Rangani, Ana Claudia Langaro, Shilpi Agrawal, Reiofeli Algodon Salas-Perez, Juan Camilo Velasquez, Christopher E. Nelson, Nilda Roma-Burgos

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Italian ryegrass (Lolium multiflorum Lam.) is a persistent weed species that poses significant management challenges in key agricultural crops such as wheat, corn, cotton, and soybean. This study investigated the prevalence of resistance to ACCase inhibitor herbicides, specifically diclofop and pinoxaden, among field-collected Italian ryegrass populations. The survey revealed widespread resistance to diclofop and emerging cross-resistance to pinoxaden. To elucidate the physiological mechanism of ACCase herbicide resistance, we investigated mutations in the carboxyl-transferase (CT) domain of the ACCase enzyme, a critical region for herbicide sensitivity. Using dCAPS assays and CT domain sequencing, several known resistance-conferring mutations were detected in …

Clomazone And Oxyfluorfen Combinations In A Flooded Rice System, Jason K. Norsworthy, Casey H. Arnold, Thomas R. Butts, Trenton L. Roberts, Chad W. Shelton

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Rice producers battle herbicide-resistant weeds worldwide while producing rice for ≥50% of the world’s population. Oxyfluorfen can provide rice producers with an alternative site of action for barnyardgrass control, as there are no documented cases of grass weeds being resistant to the herbicide in the mid-southern United States. Oxyfluorfen is anticipated to be labeled in the Roxy Rice Production System and may be sold as a clomazone/oxyfluorfen premixture; hence, experiments were conducted in 2021 and 2022 to evaluate preemergence-applied clomazone/oxyfluorfen ratios compared to clomazone alone on silt loam and clay soils. All ratios of the herbicides caused less than 7% …

Effect Of Heat Stress On The Expression Of Circulating Cyto(Chemo)Kine And Inflammatory Markers In Broiler Chickens Selected For High- Or Low-Water Efficiency, Elizabeth S. Greene, Travis Tabler, Walter G. Bottje, Sara Orlowski, Sami Dridi

Poultry Science Faculty Publications and Presentations

Background:

Water scarcity is a current, significant global concern that will only increase under the pressure of climate change. Improving water efficiency of poultry is a new and promising area to help temper agriculture's future impact on fresh water availability. Here, we explored the effects of acute heat stress (HS) on circulating stress and inflammatory markers in 2 lines of broilers divergently selected for water efficiency. Methods:

Male chicks from low (LWE) and high water efficient (HWE) lines were raised in 12 environmental chambers (2 pens/chamber, 6 chambers/line, 20 birds/pen) under normal conditions until day 28. On day 29, birds …

Food Science Affiliated Publications, University Of Arkansas, Fayetteville: Snapshot Of The Research Publications, Jeremy J. Smith, Lutishoor Salisbury

University Libraries Faculty Publications and Presentations

This report provides a snapshot of the research publications for authors affiliated with the Food Science department at the University of Arkansas, Fayetteville, for the period 2018-2023. It includes publications by material type; co-author characteristics; ranking of journals according to journal citation reports.

Crystal Archer: Profile Of The Newest Chbc Faculty Member, Danna Jaimes-Fajardo, Jeremy J. Smith, Lutishoor Salisbury

University Libraries Faculty Publications and Presentations

Presents the research profile of Crystal Archer, Assistant Professor, Chemistry and Biochemistry department. It highlights her research interests, education, and impact of her research.

Ontogenetic Biomechanics Of Tufted (Sapajus) And Untufted (Cebus) Capuchin Mandibles, Taylor A. Polvadore, Caitlin B. Yoakum, Parker M. Taylor, Megan A. Holmes, Myra F. Laird, Janine Chalk-Wilayto, Claudia Misue Kanno, Jose Americano De Oliveira, Claire E. Terhune

Anthropology Faculty Publications and Presentations

Objectives

Cortical bone geometry is commonly used to investigate biomechanical properties of primate mandibles. However, the ontogeny of these properties is less understood. Here we investigate changes in cortical bone cross-sectional properties throughout capuchin ontogeny and compare captive versus wild, semi-provisioned groups. Tufted capuchins (Sapajus spp.) are known to consume relatively hard/tough foods, while untufted capuchins (Cebus spp.) exploit less mechanically challenging foods. Previous research indicates dietary differences are present early in development and adult Sapajus mandibles can resist higher bending/shear/torsional loads.

Materials and methods

This study utilized microCT scans of 22 Cebus and 45 Sapajus from early …

Polyphenol-Induced Protein Structural Modifications In Sorghum On Pasting Properties, Annegret Jannasch, Ya-Jane Wang, Sun-Ok Lee, Rohana Liyanage, Zeina Alraawi, Suresh Kumar Thallapuranam, William L. Rooney

Chemistry & Biochemistry Faculty Publications and Presentations

Black sorghum contains a high amount of anthocyanins and proanthocyanidins, whereas white sorghum predominantly comprises phenolic acids. Polyphenols covalently and non-covalently interact with protein, whereas the structure of both compounds affects these interactions. This study investigated the interactions between phenolic acids, anthocyanins, proanthocyanidins and prolamin, which is the major protein fraction in sorghum, and their effect on the pasting properties of white and black, whole and milled sorghum using different chemical agents. Urea increased the pasting viscosity of black sorghum, but not that of white sorghum, indicating that hydrogen bonds and hydrophobic interactions between prolamin and anthocyanins and proanthocyanidins play …

Simultaneous Modulation Of Pulse Charge And Burst Period Elicits Two Differentiable Referred Sensations, T. R. Benigni, A. E. Pena, S. S. Kuntaegowdanahalli, J. J. Abbas, R. Jung

Biomedical Engineering Faculty Publications and Presentations

Objective. To investigate the feasibility of delivering multidimensional feedback using a single channel of peripheral nerve stimulation by complementing intensity percepts with flutter frequency percepts controlled by burst period modulation. Approach. Two dimensions of a distally referred sensation were provided simultaneously: intensity was conveyed by the modulation of the pulse charge rate inside short discrete periods of stimulation referred to as bursts and frequency was conveyed by the modulation of the period between bursts. For this approach to be feasible, intensity percepts must be perceived independently of frequency percepts. Two experiments investigated these interactions. A series of two alternative forced …