Life Sciences Commons^™

Determination Of P Availability In Feedstuffs And The Effect Of Feedstuffs And Anti-Nutritional Factors On Secretory Iga Production And Host Nutrient Losses In Poultry, Benjamin Parsons

Graduate Theses and Dissertations

The availability of P in feedstuffs and the effect of feedstuffs, feed additives, and anti-nutritional factors on secretory IgA (sIgA) production in chickens were evaluated. A rapid method was developed using the precision-fed rooster assay to evaluate phytic acid disappearance, P digestibility, and phytase efficacy. Further, exogenous phytase was found to be effective at degrading myo-inositol 1,2,3,4,5,6-hexakis, however, the enzyme was less efficient at P release from lower inositol phosphates. The availability of P in feedstuffs was found to be affected by dietary Ca content, where increasing Ca linearly reduced (P < 0.05) both phytic acid hydrolysis and P digestibility. For example, the standardized ileal digestibility of P was reduced from 73 to 47% in soybean meal when the dietary Ca content was increased from 0.30 to 0.75%. The digestibility of P in distillers dried grains with solubles (DDGS) and corn fermented protein (CFP), however, was high across all Ca levels (71 to 80%). Relative P bioavailability based on tibia bone ash was also found to be high for DDGS and CFP, being 81 and 78%, respectively. Upon further evaluation, it was observed that utilizing low dietary Ca levels (0.30%) when P digestibility in individual plant-based feedstuffs was determined overestimated the P digestibility in practical grower diets by up to 16 percentage units. Utilization of higher Ca levels (0.75%) when P digestibility in individual plant-based feedstuffs was determined, however, resulted in calcualted P digestibility values that were within 1 to 3 percentage units of the measured digestibility in practical grower diets. Aside from P, feedstuffs were also evaluated to determine their effect on sIgA production in the intestine. Roosters excreted approximately 6 to 30 mg of sIgA / 24 h. Further, the proportional contribution of sIgA to total endogenous amino acid (AA) losses ranged from 1 to 13%, with the greatest contribution being observed for Trp. The addition of casein to a protein-free diet increased (P < 0.05) sIgA production in both roosters and broiler chickens. There was no change (P > 0.05) in sIgA production compared with the protein-free diet …

Detection Of Dsrab Operon Expression In Desulfotalea Psychrophila Cells Subjected To Simulated Martian Conditions Of Temperature And Regolith's Sulphate Minerals Composition, Sergio Mosquera, Mack Ivey, Vincent F. Chevrier

Biological Sciences Faculty Publications and Presentations

Discoveries of transient liquid water in the Martian polar caps and the presence of liquid lakes and subsurface oceans in icy satellites have increased the interest of scientists in the capabilities of terrestrial extremophiles to grow and remain metabolically active in these extreme environments. The principal goal of this research is to understand the metabolic capacity of the anaerobic psychrophile, Desulfotalea psychrophila, cultured at subfreezing temperatures in media containing various concentrations of sulphate minerals. In this regard, our experiments focused on the detection of D. psychrophila survival and active metabolism, employing a biochamber that can recreate Martian temperatures. Using …

Controlling Salmonella: Strategies For Feed, The Farm, And The Processing Plant, Tomi Obe, Timothy Boltz, Mike Kogut, Steven C. Ricke, Lasheda A. Brooks, Ken Macklin, Ashley Peterson

Poultry Science Faculty Publications and Presentations

Controlling Salmonella in poultry is an ongoing food safety measure and while significant progress has been made, there is a need to continue to evaluate different strategies that include understanding Salmonella-poultry interaction, Salmonella-microbiota interactions, Salmonella genetics and response to adverse conditions, and preharvest and postharvest parameters that enable persistence. The purpose of this symposium is to discuss different strategies to consider from feed milling to the farm to the processing environment. This Poultry Science Association symposium paper is divided into 5 different sections that covers 1) immunological aspects of Salmonella control, 2) application of Salmonella genetics for targeted …

Effects Of Landcover On Mesocarnivore Density And Detection Rate Along An Urban To Rural Gradient, Leah E. Mctigue, Brett Degregorio

Biological Sciences Faculty Publications and Presentations

Human development has major implications for wildlife populations. Urban-exploiter species can benefit from human subsidized resources, whereas urban-avoider species can vanish from wildlife communities in highly developed areas. Therefore, understanding how the density of different species varies in response to landcover changes associated with human development can provide important insight into how wildlife communities are likely to change and provide a starting point for predicting the consequences of those changes. Here, we estimated the population density of five common mesocarnivore species (coyote (Canis latrans), bobcat (Lynx rufus), red fox (Vulpes vulpes), raccoon (Procyon …

Design Of An Fgf-1-Fgf-2 Heterodimer Variant With Enhanced Stability And Cell Proliferation Activity, Nicholas Alan Pohlman

Chemistry & Biochemistry Undergraduate Honors Theses

The FGF-1 subfamily, composed of FGF-1 and FGF-2, assists in broad health-related processes such as cell proliferation and angiogenesis respectively.^1,2 The subfamily shows promising signs as a therapeutic, however, the inherent thermal instability leads to a low half-life in vivo.³ To assist in improving the stability, FGF-1 and FGF-2 were connected via a 12-residue glycine linker ultimately producing a heterodimer. This heterodimer was further examined to discover its novel properties. Inspiration for this project was drawn from previous research which performed five mutations on FGF-1 ultimately improving stability in the protein complex. In addition to these five …

Assessing The Feasibility Of Implementing A Dairy Industry In Mauritius, Sophia Ultes

Economics Undergraduate Honors Theses

Mauritius faces food security and economic challenges due to its lack of a domestic dairy industry. To address this, the University of Arkansas partnered with the Ministry of Agro Industry and Food Security to explore the possibility of establishing a dairy sector on the island.

Through surveys and interviews with locals, including restaurant owners and grocery store customers, we investigated the demand for fresh milk. We also studied factors like consumer preferences and production costs. Our research, conducted with IRB approval, aimed to understand if people are willing to buy locally produced milk.

During the summer, our team visited Mauritius …

Patterns In Winter Stonefly Distribution Along A River Continuum And Land-Use Gradient In Northwest Arkansas Streams, Zachary Tipton

Graduate Theses and Dissertations

Freshwater ecosystems are facing a crisis with extinction rates of aquatic species exceeding those of their terrestrial counterparts by up to fivefold. This decline is predominantly attributed to evolving land use patterns within watersheds, leading to chemical and physical transformations in freshwater habitats. Northwest Arkansas (NWA) represents one of the fastest-growing regions in the United States, undergoing substantial shifts in land use. Consequently, the status of aquatic life in this region remains uncertain. Addressing this concern, the latest Arkansas Wildlife Action Plan emphasizes the necessity of distribution and population data to guide conservation efforts for Species of Greatest Conservation Need …

Effect Of Cultural Strategies And Application Technologies On Weed Control In Flooded And Furrow-Irrigated Rice, Noah Hunter Reed

Graduate Theses and Dissertations

Weed control is a major problem that rice (Oryza sativa L.) producers face in the Mid-south every growing season. Restricted use of some rice herbicides has also highlighted the need for more research to be conducted on cultural weed control strategies and application technologies in rice. The aim of these studies was to evaluate rice weed control using various rice row widths, bed widths (irrigation-furrow spacing), rice cultivar selection, and nozzle type selection. Three field experiments were conducted in 2021 and 2022 across four sites at Lonoke, AR, Pine Tree, AR, Rohwer, AR, and Stoneville, MS. In experiment one, as …

Impact Of Reducing Ammonia Concentration By Ozone Technology And Its Potential Improvement On Broiler Performance, Odilpio Quintana Ortega

Graduate Theses and Dissertations

The employment of ozone in livestock production as a means of mitigating ammonia is used for enhancing air quality within animal confinement facilities, such as swine or poultry barns. Ammonia is a byproduct of animal excrement. Elevated ammonia concentrations can adversely affect the well-being of both animals and humans. The aim of this thesis was to investigate the effects of Ozone Technology Machine Unit (OTMU) utilization in poultry barns regarding ammonia concentration and its potential benefit in terms of broilers performance and welfare. In recent years, there has been a significant boom in technological advances. New devices that help mitigate …

Characteristics Of Award-Winning Children’S Books About Agriculture: An Analysis Of Content, And The Perspectives Of Authors, Illustrators, And Publishers, Addison L. Beckham

Graduate Theses and Dissertations

The purpose of this two-article qualitative study was to characterize children's literature about agriculture and to describe the perceptions of authors and illustrators who are responsible for writing and designing these successful publications. This will result in the ability of organizations like Feeding Minds Press to provide writers, illustrators, and publishers with effective strategies and techniques to improve the accuracy and overall quality of children’s literature about agriculture. Few parameters exist for authors of children’s books about agriculture (Biser, 2007). These parameters are necessary to ensure the quality and accuracy of these educational efforts (Serafini, 2012). Though Feeding Minds Press …

Habitat Associations Of Plains Spotted Skunk (Spilogale Interrupta) Across The Plains Ecoregion. And The Landcover Occupancy Models Of The Gray Fox (Urocyon Cinereoargenteus) In The Southeastern Plains Region In Arkansas, Michael Shaw

Graduate Theses and Dissertations

Much of the landscape of the United States has been altered by humans, leading to the eradication of most populations of large mammalian predators. Consequently, many species of mammalian mesopredators (medium-sized mammalian predators) have come to dominate the landscape. Species such as the gray fox (Urocyon cinereoargenteus) may appear to be on the rise and doing well due to their widespread range, but at a local scale may need monitoring and less is known in areas of its range. Others such as the plains spotted skunk have a wide range across the plains but are on the decline even with …

Exploring The Interrelationships Among Structural Complexity, Multi-Trophic Biodiversity, And Ecosystem Productivity In Forested Ecosystems., Ayanna St. Rose

Graduate Theses and Dissertations

Forests play a pivotal role in sustaining global biodiversity and mitigating the global climate, as they serve as the primary refuge for terrestrial biodiversity and effectively capture and store atmospheric carbon dioxide. However, changing climate and land use are altering the geographic distribution, physical structure, and productivity of forested ecosystems that can have profound impacts on biodiversity conservation, forest productivity, and global climate. This dissertation contributes to our understanding of multifaceted interactions between the physical and biological structural complexity and ecosystem functions in forested landscapes across the United states by using openly available data from two national networks: the National …

Assessment Of Dietary Energy And Amino Acid Diet Formulation In The Modern Broilers, Matheus Freitas Costa

Graduate Theses and Dissertations

As a result of the advancement of genetics, understanding broiler requirements for dietary energy and amino acids is essential for improving live performance and carcass characteristics. The energy system used to formulate diets in poultry is based on metabolizable energy (ME); this equation can be described as the total amount of energy in the feed minus gases and feces. However, the net energy (NE) system may be the best depiction of the energy utilized by birds for production and maintenance purposes once the heat increment (HI) is measured. Thus, a series of studies were conducted to establish the responsiveness of …

Human-Bat Interactions In A Disease Emergence Hotspot: Implications For Human Health And Bat Conservation, Reilly Tempest Jackson

Graduate Theses and Dissertations

Bats are an ecologically important taxon that can host zoonotic pathogens. Globally, many bat species are synanthropic and live closely with humans, often roosting in man-made structures. The spatial overlap between humans and bats creates opportunities for human-bat contact, which can lead to human exposure to bat-borne pathogens and conflicts that cause bat mortality. Despite this risk, little is known about the drivers and characteristics of these human-bat interactions in buildings and work is needed to understand this aspect of the wildlife-urban interface. In Chapter I, I present a literature review that identifies the geographic and taxonomic trends in reported …

Age, Size, And Composition Of Selected Old-Growth Shortleaf Pine Stands In The Upper Buffalo River, Arkansas, Willa Avery Thomason

Graduate Theses and Dissertations

Shortleaf pine (Pinus echinata) approaches the westernmost limits of its range in the Arkansas Ozarks. Despite heavy logging of shortleaf pine throughout its range during the 19th and 20th centuries, the species was not easily accessible for harvest and transportation in the most rugged areas of the Boston Mountains and, as a result, there are a few extant stands of old-growth shortleaf pine in the region. The shortleaf pine stands in the interior Boston Mountains are unusual in that they exist primarily above bluff lines at topographic breaks in the hardwood canopy and are not disturbed by fire as frequently …

Health Beneficial Effects Of Black Raspberry, Blueberry, And Blackberry Volatile Extracts In Lung And Colon, Inah Gu

Graduate Theses and Dissertations

Berries are one of the most consumed fruits in our diet, and rich in dietary fiber, vitamins, minerals, polyphenols, and volatiles. Volatiles are responsible for the aroma of berries, and are widely used in food, perfumery, and cosmetic industries. However, the potential health beneficial effects of berry volatiles have not been extensively studied. Recently, we found that berry volatiles from cranberries, black and red raspberries, strawberries, blueberries, and blackberries have an anti-inflammatory effect on inflamed murine macrophage cells. Since inflammation can develop into many chronic diseases, and berry volatiles can be inhaled or consumed and pass through the lungs or …

Effect Of Onion Peel Bioactive Compounds On Gut Microbiome Of Obese Adults, Saad Al-Din Sifat

Graduate Theses and Dissertations

Obesity, a pervasive global health concern, involves a complex interplay between genetic factors, dietary patterns, and the gut microbiota. Onion peels are rich in polyphenols and known for their antioxidant properties. However, the effects of in-vitro digested onion peel extract has not been investigated thus far. This study therefore assesses the effects of digested bioactive compounds from onion peels, emphasizing gut microbiota shift and metabolites. Onion peel extract (OPE), digested onion peel extract (DOPE), and solid-phase-extracted digested onion peel extracts (SPE-DOPE) were analyzed for total phenolic content (TPC) and antioxidant activity. SPE-DOPE exhibited the highest TPC and antioxidant activity. Fecal …

Solar Cooking Solutions For Haiti, Isaac Mergner

Graduate Theses and Dissertations

This study focuses on enhancing the efficiency of solar cooking through the use of solar cookers and heat retention boxes. The research involved testing various cooking vessels and lids in a solar cooking experiment, with a specific focus on the performance of different materials and their heat retention capabilities. The experiment aimed to increase the efficiency of solar cookers, which are popular in developing nations due to their relatively low cost. The findings indicate that the combination of a solar cooker and a heat retention box can significantly improve the cooking process, particularly in areas with limited access to cooking …

The Effects Of Probiotics And Environment On The Swine Gut Microbiome, Bin Zuo

Graduate Theses and Dissertations

The gastrointestinal tract is crucial for nutrient absorption and immune system functionality. It employs a synergistic approach, merging physical and biochemical defenses, to counteract pathogens that enter through dietary or other external routes. This system also facilitates the breakdown of complex carbohydrates, a process beyond the host's innate capacity. In swine, gut microbiota early colonization plays a significant role in several physiological developments, shaped by a variety of determinants including maternal factors and environmental conditions. In the swine industry, the shift to indoor pig farming enhances efficiency but limits soil interaction, potentially impacting gut microbiota diversity. Additionally, the practice of …

Evaluation Of Weed Control Efficacy And Crop Tolerance To Florpyrauxifen-Benzyl Coated Onto Urea, Bodie Lyn Cotter

Graduate Theses and Dissertations

Frequent off-target movement occurrences to soybean [Glycine max (L.) Merr.], rice (Oryza sativa L.) injury, and a lack of barnyardgrass [Echinochloa crus-galli (L.) P. Beauv.] control followed the commercial launch of florpyrauxifen-benzyl in 2018. Florpyrauxifen-benzyl is a synthetic auxin, Herbicide Resistance Action Committee (HRAC)/Weed Science Society of America (WSSA) Group 4 herbicide used for postemergence weed control in rice and labeled for application at 30 g ae ha-1. Previous research documented that soybean was susceptible to injury following spray applications of florpyrauxifen-benzyl at less-than-labeled rates, and certain varieties of rice are less tolerant to spray-applied florpyrauxifen-benzyl at 30 g ae …

Interactions Between Fitness Components Across The Life Cycle Constrain Competitor Coexistence, Miguel Gómez-Llano, Wade A. Boys, Taylor Ping, Simon P. Tye, Adam M. Siepielski

Biological Sciences Faculty Publications and Presentations

1. Numerous mechanisms can promote competitor coexistence. Yet, these mechanisms are often considered in isolation from one another. Consequently, whether multiple mechanisms shaping coexistence combine to promote or constrain species coexistence remains an open question.
2. Here, we aim to understand how multiple mechanisms interact within and between life stages to determine frequency-dependent population growth, which has a key role stabilizing local competitor coexistence.
3. We conducted field experiments in three lakes manipulating relative frequencies of two Enallagma damselfly species to evaluate demographic contributions of three mechanisms affecting different fitness components across the life cycle: the effect of resource competition on individual growth …

Sensory Input To Cortex Encoded On Low-Dimensional Periphery-Correlated Subspaces, Andrea K. Barreiro, Antonio J. Fontenele, Cheng Ly, Prashant Chama Raju, Shree Hari Gautam, Woodrow L. Shew

Physics Faculty Publications and Presentations

As information about the world is conveyed from the sensory periphery to central neural circuits, it mixes with complex ongoing cortical activity. How do neural populations keep track of sensory signals, separating them from noisy ongoing activity? Here, we show that sensory signals are encoded more reliably in certain low-dimensional subspaces. These coding subspaces are defined by correlations between neural activity in the primary sensory cortex and upstream sensory brain regions; the most correlated dimensions were best for decoding. We analytically show that these correlation-based coding subspaces improve, reaching optimal limits (without an ideal observer), as noise correlations between cortex …

Mapping Variability Of Soybean Leaf Potassium Concentrations To Develop A Sampling Protocol, Carrie C. Ortel, Trenton Roberts, Kyle A. Hoegenauer, Aurelie M. Poncet, Nathan A. Slaton, W. Jeremy Ross

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

The spatial variability of soybean [Glycine max (L.) Merr.] leaf-K (potassium) concentrations must be considered when collecting samples to monitor crop K status. Five commercial soybean fields were sampled at a 0.4-ha grid resolution at two reproductive growth stages to quantify the trifoliolate tissue-K concentration. The objectives of this study were to identify the potential field variability of soybean leaf-K concentrations in typical Mid-south US soybean production fields, evaluate interpolation methods, and develop a sampling protocol for in-season soybean tissue monitoring. No consistent spatial dependencies were found in the leaf-K concentrations across the fields and sample times, indicating that …

Uncertainty In Streamflow Measurements Significantly Impacts Estimates Of Downstream Nitrate Export, Shannon L. Speir, C. Nathan Jones, Arial J. Shogren, Carla L. Atkinson

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Across watershed science, two key variables emerge–streamflow and solute concentration–which serve as the basis for efforts ranging from basic watershed biogeochemistry research to policy decisions surrounding watershed management. However, we rarely account for how error in discharge (Q) impacts estimates of downstream nutrient loading. Here, we examined the impact of uncertainty in streamflow measurements on estimates of downstream nitrate export using publicly available data from the U.S. Geological Survey (USGS). We characterized how uncertainty in stage-discharge relationships impacts annual flux estimates across 70 USGS gages. Our results indicate the interquartile range of relative error in Q was 33% across these …

The Pseudomonas Syringae Pv. Tomato Dc3000 Effector Hopd1 Interferes With Cellular Dynamics Associated With The Function Of The Plant Immune Protein Atnhr2b, Luis Francisco Marin-Ponce, Catalina Rodriguez-Puerto, Perla Rocha-Loyola, Clemencia M. Rojas

Entomology and Plant Pathology Faculty Publications and Presentations

The plant pathogenic bacterium Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) causes disease in tomato, in the model plant Arabidopsis thaliana, and conditionally in Nicotiana benthamiana. The pathogenicity of Pst DC3000 is mostly due to bacterial virulence proteins, known as effectors, that are translocated into the plant cytoplasm through the type III secretion system (T3SS). Bacterial type III secreted effectors (T3SEs) target plants physiological processes and suppress defense responses to enable and support bacterial proliferation. The Pst DC3000 T3SE HopD1 interferes with plant defense responses by targeting the transcription factor NTL9. This work shows that HopD1 also targets the …

Innovative Approaches To Poultry Processing Wastewater Treatment: The Stainless Steel Ultrafiltration Membrane As A Viable Option, Saubana Olorunrola Dada, Chidambaram Thamariselvan, Mahmood Jebur, Sumith Ranil Wickramasinghe

Chemical Engineering Faculty Publications and Presentations

In pursuit of sustainability, we explored replacing conventional dissolved air floatation (DAF) in poultry processing wastewater (PPW) treatment with a precisely tuned 0.02 µm stainless-steel ultrafiltration (SSUF) membrane. SSUF is a robust, homogenously porous membrane with strong chemical resistance, ease of cleaning, and exceptional resistance to organic fouling. Unlike polymeric membranes, it can be regenerated multiple times, making it a cost-effective choice due to its compatibility with harsh chemical cleaning. The PPW used for the study was untreated wastewater from all processing units and post-initial screening. Our study revealed the SSUF membrane’s exceptional efficiency at eliminating contaminants. It achieved an …

Editorial: Respiratory Microbiome In Health And Disease, Yimin Zhuang, Tao Ding, Jiangchao Zhao, Jianmin Chai

Animal Science Faculty Publications and Presentations

Editorial on the Research Topic:

Respiratory microbiome in health and disease

The Spatial Dissimilarities And Connections Of The Microbiota In The Upper And Lower Respiratory Tract Of Beef Cattle, Zhihao Zhang, Chengqian Zhang, Yikai Zhong, Shuli Yang, Feilong Deng, Ying Li, Jianmin Chai

Animal Science Faculty Publications and Presentations

Bovine respiratory disease (BRD) causes morbidity and mortality in cattle. The critical roles of the respiratory microbiota in BRD have been widely studied. The nasopharynx was the most popular sampling niche for BRD pathogen studies. The oral cavity and other niches within the respiratory tract, such as nostrils and lung, are less assessed. In this study, oropharyngeal swabs (OS), nasal swabs (NS), nasopharyngeal swabs (NP), and bronchoalveolar lavage (BAL) were collected from calves located in four countries and analyzed for investigation of the dissimilarities and connections of the respiratory microbiota. The results showed that the microbial diversity, structure, and composition …

An Amplification-Free Crispr-Sers Biosensor For Specific, Sensitive And Rapid Detection Of Salmonella Typhimurium In Poultry, Fei Jia, Bingyan Li, Yawen He, Yafang Shen, Juhong Chen, Xingmin Li, Yanbin Li

Biological and Agricultural Engineering Faculty Publications and Presentations

CRISPR (Clustered regularly interspaced short palindromic repeats) has been a cutting-edge biotechnology in both gene editing and biosensing due to its desirable features, such as high base resolution and set-and-forget operation. However, as a nucleic acid detection method, an DNA/RNA amplification procedure is still inevitable in CRISPR biosensors. In this study, a highly-ordered nanopore array SERS substrate, coated with silver nanoparticles, was fabricated and utilized as a platform for the specific detection of Salmonella Typhimurium (S. Typhimurium) without the time-consuming DNA amplification step. The smart sensing strategy was established by introducing an ssDNA labeled with a Raman active molecule …

University Of Arkansas, Chemistry And Biochemistry Department Graduate Student Publications, 2018- November 2023. 39p., Jeremy Smith, Lutishoor Salisbury

University Libraries Faculty Publications and Presentations

This report provides a compilation of the research publications by the Chemistry and Biochemistry (CHBC) Graduate students for the period: 2018-November 2023. It includes publications by the CHBC graduates and those where a CHBC faculty was the main advisor. It includes a summary of the research. The listing is organized according to type of publications within specific years.