Life Sciences Commons^™

The Subcloning And Expression Of Munc18a In Escherichia Coli For Antibody Production And Analysis In Mast Cell Degranulation Reactions, Brandi Goble

Honors Theses

Mast cells are secretory cells responsible for fighting off infection through the early recognition of pathogens. This process is completed through the secretion of proinflammatory mediators that are stored in secretory granules within the cytoplasm of the cell. The degranulation secretion process relies on regulated fusion of secretory granules to the cell membrane via membrane-bound SNARE proteins that bridges the two opposed membranes. The intricate regulation of SNARE-mediated mast cell degranulation is not well understood. However, Sec1/Munc18 (SM) proteins, specifically the Munc18 isoforms, are known to play a critical role in the process (Brochetta, et. al., 2014). The Xu lab …

Disruption Of Rna Metabolism By Zika Virus, Maggie Lea Dickerson

Honors Theses

Flaviviruses are positive, single-stranded, enveloped RNA viruses that are a part of the family, Flaviviridae. West Nile virus, Dengue, Zika virus and more are a part of this family. Mosquitoes are the vectors for these viruses. In order for the virus to infect mosquitoes, it must evade the RNA interference (RNAi), which is the major antiviral immune mechanism of insects. One study found the 3’ untranslated region (UTR) of the West Nile virus that inhibited the RNAi (GP et al. 2016). The goal of this study is to investigate if the 3’ and 5’ UTR region of the Zika virus …

Interactions Of Fire And Herbivory On The Management Of Ilex Glabra In Longleaf Pine Forests, Courtney R. Filliben

Honors Theses

The longleaf pine forest is characterized by the high levels of biodiversity and species richness that give it both ecological and economical importance. Decades of clear-cutting, habitat fragmentation, and natural fire restriction have reduced this once great forest system to a mere fraction of its original range. One of the specific causes of the decline of the longleaf pine is understory domination by woody shrubs. These shrubs limit the growth of characteristic grasses and forbs that are responsible for the majority of the biodiversity. The overall purpose of this study was to identify a new longleaf pine reforestation technique that …

Sibship Reconstruction For Inferring The Number Of Breeders Of Gulf Sturgeon In The Apalachicola River, Robbilyn Verges

Honors Theses

The Gulf sturgeon is an anadromous fish that inhabits the Gulf of Mexico and its neighboring river drainages. The species is currently listed as threatened due to habitat alterations and overfishing. In this study, we focused on the Apalachicola River in Florida, which has had several historic spawning locations of the sturgeon blocked by the Jim Woodruff Lock and Dam. Age-1 juvenile sturgeon from the year 2013 (n=31) and 2014 (n=131) were genotyped using fourteen microsatellite loci. Sibship reconstruction and parentage assignment was performed in order to determine the effective number of breeders (Nb) and the total number of spawning …

Effect Of American Football Helmet Chin Strap Placement On Measures Of Blunt Impact, Terrie Lane Jackson

Honors Theses

Approximately 1.8 to 3.6 million concussions occur annually across all sports.3 Although concussion is prevalent and is a growing concern, the mechanism behind this brain injury is still vaguely understood. The purpose of this research was to determine if chinstrap attachment location effects standard measures of helmet performance associated with concussion. To test this, three Schutt F7 American Football Helmets were impacted at the front, side, and rear locations under two conditions (high and low chinstrap attachment). Each helmet was fitted to an anthropometric test device outfitted with a 3-2-2-2 accelerometer and impacted by a steel impactor head affixed to …

Head Impact Biomechanics Of Collegiate Football Players, Samantha Eshleman

Honors Theses

This research study focused on the descriptive head impact biomechanics of collegiate American football players. The purpose of this study was to determine if there were statistical differences in the frequency, peak linear acceleration, and peak rotational acceleration between player position and impact location on the helmet during practice sessions. There were 31 players from the University of Southern Mississippi’s Division I football team that participated in the study. Participants were divided into four groups based on position: defensive skill, defensive line, offensive skill, and offensive line. The Head Impact Telemetry (HIT) System was incorporated with the Sideline Response System …

Genetic Estimates Of The Number Of Breeding Adults In Alligator Gar From The Choke Canyon Reservoir, Texas, E. Blayne Newsome

Honors Theses

Alligator gar were historically found across the coastal drainages of the Gulf of Mexico and up into the Mississippi River basin. However, their populations are experiencing decline in many portions of their range. Texas seems to have large populations of alligator gar, but state resource officers are seeking to better understand its biology so as to provide appropriate management recommendations to maintain the recreational fishery. In this study, I used genetic techniques to examine a cohort of 144 juvenile alligator gar collected in 2016 in the Choke Canyon Reservoir. By estimating the effective number of breeders and number of spawning …

Evaluating The Role Of Large Jellyfish And Forage Fishes As Energy Pathways, And Their Interplay With Fisheries, In The Northern Humboldt Current Systems, Luciano M. Chiaverino, Kelly L. Robinson, Jorge Tam, James J. Ruzicka, Javier Quiñones, Katrina Aleksa, Frank J. Hernandez Jr., Richard D. Brodeur, Robert T. Leaf, Shin-Ichi Uye, Mary Beth Decker, Marcelo Acha, Hermes Mianzan, William M. Graham

Faculty Publications

Large jellyfish are important consumers of plankton, fish eggs and fish larvae in heavily fished ecosystems worldwide; yet they are seldom included in fisheries production models. Here we developed a trophic network model with 41 functional groups using ECOPATH re-expressed in a donor-driven, end-to-end format to directly evaluate the efficiency of large jellyfish and forage fish at transferring energy to higher trophic levels, as well as the ecosystem-wide effects of varying jellyfish and forage fish consumption rates and fishing rates, in the Northern Humboldt Current system (NHCS) off of Peru. Large jellyfish were an energy-loss pathway for high …

High Male Sexual Investment As A Driver Of Extinction In Fossil Ostracods, Maria João Fernandes Martins, T. Markham Puckett, Rowan Lockwood, John P. Swaddle, Gene Hunt

Faculty Publications

Sexual selection favours traits that confer advantages in the competition for mates. In many cases, such traits are costly to produce and maintain, because the costs help to enforce the honesty of these signals and cues. Some evolutionary models predict that sexual selection also produces costs at the population level, which could limit the ability of populations to adapt to changing conditions and thus increase the risk of extinction. Other models, however, suggest that sexual selection should increase rates of adaptation and enhance the removal of deleterious mutations, thus protecting populations against extinction. Resolving the conflict between these models is …

A Plant-Produced Vaccine Protects Mice Against Lethal West Nile Virus Infection Without Enhancing Zika Or Dengue Virus Infectivity, Huafang Lai, Amber M. Paul, Haiyan Sun, Junyun He, Ming Yang, Fengwei Bai

Faculty Publications

West Nile virus (WNV) has caused multiple global outbreaks with increased frequency of neuroinvasive disease in recent years. Despite many years of research, there are no licensed therapeutics or vaccines available for human use. One of the major impediments of vaccine development against WNV is the potential enhancement of infection by related flaviviruses in vaccinated subjects through the mechanism of antibody-dependent enhancement of infection (ADE). For instance, the recent finding of enhancement of Zika virus (ZIKV) infection by pre-exposure to WNV further complicates the development of WNV vaccines. Epidemics of WNV and the potential risk of ADE by current vaccine …

Genetic Homogeneity Of The Invasive Lionfish Across The Northwestern Atlantic And The Gulf Of Mexico Based On Single Nucleotide Polymorphisms, R. Pérez-Portela, A. Bumford, B. Coffman, S. Wedelich, M. Davenport, Alex Fogg, M.K. Swenarton, F. Coleman, M.A. Johnston, D.L. Crawford, M.F. Oleksiak

Student Publications

Despite the devastating impact of the lionfish (Pterois volitans) invasion on NW Atlantic ecosystems, little genetic information about the invasion process is available. We applied Genotyping by Sequencing techniques to identify 1,220 single nucleotide polymorphic sites (SNPs) from 162 lionfish samples collected between 2013 and 2015 from two areas chronologically identified as the first and last invaded areas in US waters: the east coast of Florida and the Gulf of Mexico. We used population genomic analyses, including phylogenetic reconstruction, Bayesian clustering, genetic distances, Discriminant Analyses of Principal Components, and coalescence simulations for detection of outlier SNPs, to understand …

Long-Term Irrigation Affects The Dynamics And Activity Of The Wheat Rhizosphere Microbiome, Dmitri V. Mavrodi, Olga Mavrodi, Liam D.H. Elbourne, Sasha Tetu, Robert F. Bonsall, James Parejko, Mingming Yang, Ian T. Paulsen, David M. Weller, Linda S. Thomashow

Faculty Publications

The Inland Pacific Northwest (IPNW) encompasses 1. 6 million cropland hectares and is a major wheat-producing area in the western United States. The climate throughout the region is semi-arid, making the availability of water a significant challenge for IPNW agriculture. Much attention has been given to uncovering the effects of water stress on the physiology of wheat and the dynamics of its soilborne diseases. In contrast, the impact of soil moisture on the establishment and activity of microbial communities in the rhizosphere of dryland wheat remains poorly understood. We addressed this gap by conducting a three-year field study involving wheat …

Descriptive Density Models Of Scyphozoan Jellyfish In The Northern Gulf Of Mexico, Katrina T. Aleksa, Redwood W. Nero, Jerry D. Wiggert, William M. Graham

Faculty Publications

Jellyfish play an important role in the food web of many coastal environments but are generally considered a nuisance to scientific fieldwork and industrial and economic trades. A better understanding of how jellyfish densities and distributions are affected by environmental parameters could elucidate population trends and provide assistance in ecological research and undesirable human interactions. In this study, abundance data for scyphozoan medusae, Chrysaora sp. and Aurelia spp., were extracted from Southeast Area Monitoring and Assessment Program trawling surveys for the northern Gulf of Mexico (nGoM) during the summer and fall months. Both in situ and satellite oceanographic measurements were …

Diverse Exocytic Pathways For Mast Cell Mediators, Hao Xu, Na-Ryum Bin, Shuzo Sugita

Faculty Publications

Mast cells play pivotal roles in innate and adaptive immunities but are also culprits in allergy, autoimmunity and cardiovascular diseases. Mast cells respond environmental changes by initiating regulated exocytosis/secretion of various biologically active compounds called mediators (e.g., proteases, amines and cytokines). Many of these mediators are stored in granules/lysosomes and rely on an intricate degranulation process for release. Mast cell stabilizers (such as sodium cromoglicate) which prevent such degranulation process have therefore been clinically approved to treat asthma and allergic rhinitis. However, it has become increasingly clear that different mast cell diseases often involve multiple mediators, which seem to rely …

No More Reason For Ignoring Gelatinous Zooplankton In Ecosystem Assessment And Marine Management: Concrete Cost-Effective Methodology During Routine Fishery Trawl Surveys, A. Aubert, E. Antajan, C. Lynam, S. Pitois, A. Pliru, S. Vaz, D. Thibault

Student Publications

Gelatinous zooplankton, including cnidarians, ctenophores, and tunicates (appendicularians, pyrosomes, salps and doliolids), are often overlooked by scientific studies, ecosystem assessments and at a management level. Despite the important economic consequences that they can have on human activities and on the marine food-web, arguments often related to the costs of monitoring or their coordination, or simply negligence, have resulted in the absence of relevant monitoring programs. A cost-effective protocol has been applied on trawling from existing fishery surveys conducted by national laboratories in England and France. The testing phase has successfully demonstrated the adequacy of such a tool to sample …

A Bio-Based Pro-Antimicrobial Polymer Network Via Degradable Acetal Linkages, Douglas V. Amato, Dahlia N. Amato, Logan T. Blancett, Olga V. Mavrodi, William B. Martin, Sarah N. Swilley, Michael J. Sandoz, Glenmore Shearer, Dmitri V. Mavrodi, Derek Patton

Faculty Publications

The synthesis of a fully degradable, bio-based, sustained release, pro-antimicrobial polymer network comprised of degradable acetals (PANDA) is reported. The active antimicrobial agent – p-anisaldehyde (pA) (an extract from star anise) – was converted into a UV curable acetal containing pro-antimicrobial monomer and subsequently photopolymerized into a homogenous thiol-ene network. Under neutral to acidic conditions (pH < 8), the PANDAs undergo surface erosion and exhibit sustained release of pA over 38 days. The release of pA from PANDAs was shown to be effective against both bacterial and fungal pathogens. From a combination of confocal microscopy and transmission electron microscopy, we observed that the released pA disrupts the cell membrane. Additionally, we demonstrated that PANDAs have minimal cytotoxicity towards both epithelial cells and macrophages. Although a model platform, these results point to promising pathways for the design of fully degradable sustained-release antimicrobial systems with potential applications in agriculture, pharmaceuticals, cosmetics, household/personal care, and food industries.

Rewired Rnai-Mediated Genome Surveillance In House Dust Mites, Mosharrof H. Mondal, Pavel Klimov, Alex S. Flynt

Faculty Publications

House dust mites are common pests with an unusual evolutionary history, being descendants of a parasitic ancestor. Transition to parasitism is frequently accompanied by genome rearrangements, possibly to accommodate the genetic change needed to access new ecology. Transposable element (TE) activity is a source of genomic instability that can trigger large-scale genomic alterations. Eukaryotes have multiple transposon control mechanisms, one of which is RNA interference (RNAi). Investigation of the dust mite genome failed to identify a major RNAi pathway: the Piwi-associated RNA (piRNA) pathway, which has been replaced by a novel small-interfering RNA (siRNA)-like pathway. Co-opting of piRNA function by …

Dietary Overlap Between Jellyfish And Forage Fish In The Northern Gulf Of Mexico, Isabella D'Ambra, William M. Graham, Ruth H. Carmichael, Frank J. Hernandez Jr.

Faculty Publications

Despite the speculations that jellyfish (hydromedusae, siphonophores, scyphomedusae and ctenophores) may compete with forage fish for prey, there are few direct comparisons of their diets. To determine the dietary overlap between Aurelia sp. (Cnidaria, Scyphozoa) and Brevoortia patronus (Goode, 1878) (Pisces, Clupeidae) in the northern Gulf of Mexico, we collected monthly samples in Louisiana, Mississippi and Alabama coastal waters (USA) during summer and early fall 2009−2010. We determined carbon and nitrogen stable isotope ratios in predators and their potential prey, including small plankton (<200 µm) and mesozooplankton (200− 2000 µm), and identified prey in the stomachs of adult Aurelia sp. and B. patronus. Trophic niche overlap was defined using the stable isotope Bayesian …

Reliability Of Pulse Waveform Separation Analysis Responses To An Orthostatic Challenge, Lee Stoner, Keeron Stone, Erik D. Hanson, James Faulkner, Simon Fryer, Daniel Credeur

Faculty Publications

Cardiovascular autonomic nervous system function can be assessed using an orthostatic challenge to induce arterial wave reflection. While arterial reflection is typically estimated using a central augmentation index, a superior estimation can be obtained using pulse wave separation analysis to estimate the aortic backward pressure wave (Pb). However, to be of value in a clinical or research setting, an assessment tool must be precise (reliable). Therefore, this study sought to determine the measurement precision of Pb responses to a modified tilt-table test. Twenty healthy adults (26.4 year (SD 5.2), 24.7kg/m² (SD 3.8), 55% female) were tested in a fasted …

Leaf Herbivory Induces Resistance Against Florivores In Raphanus Sativus, Andrew C. Mccall, Samantha Case, Kelsy Espy, Grant Adams, Stephen J. Murphy

Student Publications

Florivory can have significant negative effects on plant fitness, driving selection for resistance traits in flowers. In particular, herbivory to leaves may induce resistance in flowers because herbivores on leaves often become florivores on flowers as plant ontogeny proceeds. The literature on inducible resistance in floral tissues is limited, so we used a series of experiments to determine whether prior leaf damage by Spodoptera exigua (Hübner) caterpillars affected florivore preference and performance on wild radish (Raphanus sativus L.). We found that Spodoptera exigua larvae preferred petals from control plants versus petals from plants exposed to prior leaf damage, and …

Temporal Migration Patterns Between Natal Locations Of Ruby-Throated Hummingbirds (Archilochus Colubris) And Their Gulf Coast Stopover Site, Theodore J. Zenzal Jr., Andrea J. Contina, Jeffrey F. Kelly, Frank R. Moore

Faculty Publications

Background

Autumn latitudinal migrations generally exhibit one of two different temporal migration patterns: type 1 where southern populations migrate south before northern populations, or type 2 where northern populations overtake southern populations en route. The ruby-throated hummingbird (Archilochus colubris) is a species with an expansive breeding range, which allows opportunities to examine variation in the timing of migration. Our objective was to determine a relationship between natal origin of ruby-throated hummingbirds and arrival at a Gulf coast stopover site; and if so, what factors, such as differences in body size across the range as well as the …

Exosomes Serve As Novel Modes Of Tick-Borne Flavivirus Transmission From Arthropod To Human Cells And Facilitates Dissemination Of Viral Rna And Proteins To The Vertebrate Neuronal Cells, Wenshuo Zhou, Michael Woodson, Biswas Neupane, Fengwei Bai, Michael B. Sherman, Kyung H. Choi, Girish Neelakanta, Hameeda Sultana

Faculty Publications

Molecular determinants and mechanisms of arthropod-borne flavivirus transmission to the vertebrate host are poorly understood. In this study, we show for the first time that a cell line from medically important arthropods, such as ticks, secretes extracellular vesicles (EVs) including exosomes that mediate transmission of flavivirus RNA and proteins to the human cells. Our study shows that tick-borne Langat virus (LGTV), a model pathogen closely related to tick-borne encephalitis virus (TBEV), profusely uses arthropod exosomes for transmission of viral RNA and proteins to the human- skin keratinocytes and blood endothelial cells. Cryo-electron microscopy showed the presence of purified arthropod/neuronal exosomes …

Pkc-Dependent Phosphorylation Of Munc18a At Ser313 In Activated Rbl-2h3 Cells, Pratikshya Adhikari, Hao Xu

Faculty Publications

Protein Kinase C (PKC) regulates the release of pro-inflammatory compounds from IgE/antigen-activated mast cells by unknown mechanisms. In this study, we show for the first time that PKC inhibitor Ro-03-0432, which inhibits RBL-2H3 exocytosis/degranulation in a concentration-dependent fashion, prevents the phosphorylation of membrane fusion factor Munc18a at Ser 313. Our study provides fresh evidence that PKC-dependent protein phosphorylation may contribute to the intricate regulation of mast cell degranulation by directly targeting the fusion factors.

Preliminary Age Estimates For Female Southern Stingrays (Hypanus Americanus) From Southwestern Florida, Usa, Abigail H.P. Hayne, Gregg R. Poulakis, Jason C. Seitz, James A. Sulikowski

Gulf and Caribbean Research

No abstract provided.

Morphological Features Of Taxonomical Value For The Identification Of Three Western Atlantic Penaeid Shrimp Genera (Decopoda: Penaeidae), Maria M. Criales, Carlos Varela

Gulf and Caribbean Research

Rostra, telsons and patterns of sternal spines were examined for Western Atlantic penaeid shrimps Metapenaeopsis, Rimapenaeus and Farfantepenaeus to facilitate the identification of postlarval and juvenile stages and clarify the patterns of sternal spines previously proposed as a useful tool for identifying penaeid postlarvae at the generic level. Examination of the telsons and rostra from closely related Metapenaeopsis and Rimapenaeus species indicated that at the postlarval stages these morphological features are very similar in shape and size. Regarding the sternal spines, Metapenaeopsis spp. bear a pair on the second thoracic plate, while Rimapenaeus spp. lack thoracic spines. The different pattern …

Preliminary Multivariate Comparison Of Coral Assemblages On Carbonate Banks In The Western Gulf Of Mexico, Rebekah Rodriguez, Erin E. Easton, Thomas C. Shirley, John W. Tunnell, David Hicks

Gulf and Caribbean Research

Hermatypic corals flourished on reefs in the Gulf of Mexico (GOM) in the late Pleistocene and early Holocene. Today, many of these relict reefs are mesophotic banks that have unique coral assemblages and provide critical habitat; however, the South Texas Banks (STB) lack quantitative surveys. Therefore, we used a remotely operated vehicle to conduct quantitative surveys of 5 banks: Baker, Aransas, Dream, Blackfish Ridge, and Harte. Coral communities, based on estimated coral densities (colonies/m²⁾, significantly differed among banks for terraces, slopes, and overall (combined terrace and slope) communities for most banks examined. Within banks, terrace and slope communities …

Clarifying The Range Of The Endangered Largetooth Sawfish In The United States, Jason C. Seitz, John D. Waters

Gulf and Caribbean Research

The United States population of the endangered Largetooth Sawfish (Pristis pristis) has a high probability of extinction. It is critical to identify areas with valid historical records as these areas may be important to the recovery of the species. The U.S. range is reported to extend as far east as Florida based on one vouchered specimen and 3 historical records from this state. Three of these reports presume a local capture location despite a lack of locality data. The vouchered specimen was presumed captured in southern Florida, but evidence suggests otherwise. Dried specimens observed in Florida were most likely imported …

Description Of A New Sound Produced By Nassau Grouper At Spawning Aggregation Sites, Timothy J. Rowell, Michelle T. Schärer, Richard S. Appeldoorn

Gulf and Caribbean Research

This study identified a new sound produced by Nassau Grouper in association with, although potentially not exclusive to, an agonistic interaction at a spawning aggregation. We have also provided a behavioral and acoustic description for identification of this sound in future studies. The discovery of a third type of sound produced by Nassau Grouper further highlights the importance of acoustic communication coupled with visual displays in fishes. Furthermore, identification of a new sound increases the ability to document the presence of this endangered species at spawning sites. Future efforts may reveal that the sound is produced within additional behavioral contexts …

Diel Activity Patterns And Movement Of Invasive Lionfish (Pterois Volitans/P. Miles) In The Florida Keys Identified Using Acoustic Telemetry, Michael Mccallister, Jeffrey Renchen, Benjamin Binder, Alejandro Acosta

Gulf and Caribbean Research

The invasion of Indo—Pacific lionfish (Pterois volitans/P. miles) throughout the Caribbean and southeastern U.S. Atlantic represents a significant ecological threat, yet few studies have examined the daily activity and movement patterns of this invasive species. In this study, passive acoustic telemetry was used to track lionfish at 4 coral reef sites in the Florida Keys. Fourteen lionfish were tagged among the 4 sites, and the total number of days tagged fish were detected ranged from 5 to 141 days. Hourly detection data revealed diel activity patterns with peaks at dawn and dusk. Mixed model analysis of detection data indicated a …

Reducing Elasmobranch Bycatch In The Atlantic Seabob (Xiphopenaeus Kroyeri) Trawl Fishery Of Guyana, Aaron Garstin, Hazel A. Oxenford

Gulf and Caribbean Research

The Atlantic seabob (Xiphopenaeus kroyeri) trawl fishery is very important to Guyana, with 88 licensed industrial vessels harvesting about 15,000 mt annually, representing Guyana’s most valuable seafood export. All vessels are already using both teleost by—catch reduction devices (BRDs) and turtle excluder devices (TEDs) to satisfy international market standards. However, the key stakeholder, the Guyana Association of Private Trawler Owners and Seafood Processors, is now seeking to access sustainable seafood markets through pursuing Marine Stewardship Council (MSC) certification. To this end, this study documents elasmobranch by—catch in the current fishery and examines the effectiveness of a modified TED …