Life Sciences Commons^™

Antiestrogens Inhibit Xenoestrogen-Induced Brain Aromatase Activity But Do Not Prevent Xenoestrogen-Induced Feminization In Japanese Medaka (Oryzias Latipes), Adam J. Kuhl, Marius Brouwer

Faculty Publications

In fish, exposure to estrogen or estrogen-mimicking chemicals (xenoestrogeus)during a critical period of development can irreversibly invert sex differentiation. In medaka, a male-to-female reversal upon exposure to a xenoestrogen is accompanied by an increase in brain aromatase expression and activity. However, whether this increase is the direct cause of sex reversal is unknown. In this study we further examined the role brain aromatase plays in genesis of developmental abnormalities in response to endocrine-disrupting chemicals (EDCs). Further, the effects of a mixture of apparent antagonistic environmentally relevant EDCs on development were examined to determine if their combined actions could lessen each …

Cytochrome P4501a Is Induced In Endothelial Cell Lines From The Kidney And Lung Of The Bottlenose Dolphin, Tursiops Truncatus, Rita Anne Garrick, Bruce R. Woodin, Joanna Y. Wilson, Bobby L. Middlebrooks, John J. Stegman

Faculty Publications

Marine mammals respond to the presence of polycyclic and planar halogenated aromatic hydrocarbons (PAH or PHAH) with the induced expression in endothelium of cytochrome P4501A1, regulated through the aryl hydrocarbon receptor (AHR) transcription factor. Physiological responses in other animals, such as edema and inflammation indicate that the endothelium may be compromised by exposure to AHR agonists, which are ubiquitous in the marine environment. In other mammals and fish the cellular and molecular consequences of exposure to AHR agonists have been elucidated in cultured endothelial cells. We have cultured and characterized cetacean endothelial cells (EC) and used them in induction studies. …

Zonal Patterns Of Delta C-13, Delta N-15 And Po-210 In The Tropical And Subtropical North Pacific, Min Chen, Laodong Guo, Qiang Ma, Yusheng Qiu, Run Zhang, E Lv, Yipu Huang

Faculty Publications

Nitrogen fixation process may supply a significant fraction of bioavailable nitrogen to surface waters, increase the oceanic sequestration of atmospheric CO2, and alter the distribution of geochemical parameters. We report a zonal pattern of delta N-15 and delta C-13 in particulate organic matter (POM), and ratios of particulate Po-210 to dissolved Po-210 along a transect through the subtropical and tropical North Pacific. Both N-15 and Po-210 signals indicated an enhanced N-2 fixation in the northwestern subtropical North Pacific. The eastward decrease of N-2 fixation along this transect testified the role of aeolian Fe and P in controlling marine N-2 fixation. …

Benthic Nutrient Flux In A Small Estuary In Northwestern Florida (Usa), Guy T. Didonato, Emile M. Lores, Michael C. Murrell, Lisa M. Smith, Jane M. Caffrey

Gulf and Caribbean Research

Benthic nutrient fluxes of ammonium (NH₄⁺), nitrite/nitrate (NO₂^- + NO₃^-), phosphate (PO₄^-3), and dissolved silica (DSi) were measured in Escambia Bay, an estuary within the larger Pensacola Bay system of northwestern Florida (USA). Our study occurred during a severe drought which reduced riverine inputs to Escambia Bay. Laboratory incubations of field-collected cores were conducted on 8 dates between June and October 2000 to estimate nutrient flux, and cores were collected from locations exhibiting a range of sediment organic matter content. NH₄⁺ flux ranged from – 48.1 to …

Feeding Habits Of Common Snook, Centropomus Undecimalis, In Charlotte Harbor, Florida, David A. Blewett, Rebecca A. Hensley, Philip W. Stevens

Gulf and Caribbean Research

We examined the feeding habits, ontogenetic and seasonal diet variations, and predator size–prey size relationships of common snook, Centropomus undecimalis, in Charlotte Harbor, Florida, through stomach contents analysis. A total of 694 stomachs were extracted from common snook (300–882 mm standard length [SL]) during a 24-month period (March 2000–February 2002); 432 stomachs contained prey items. At least 37 prey taxa were identified, including 19 that had not been previously reported. Fishes made up 71% of the prey by number and 90% by weight. Three prey items made up almost 50% of the diet numerically—Lagodon rhomboides, Anchoa spp., …

Comparison Of Fish Assemblages And Water Quality In Two Marinas In The British Virgin Islands, Brian Gratwicke, Martin R. Speight

Gulf and Caribbean Research

Eutrophication is a widespread problem in tropical marine environments that leads to the increase of nutrients in a water body, usually nitrate and phosphate, and is usually associated with the discharge of untreated sewage, intensive farming or fertilizer-enriched agricultural runoff (Wu 1999). Common symptoms are increased N and P levels, increased macroalgal production in shallow areas, reduced dissolved oxygen, loss of seagrass and coral habitats and changes in the fish community (Hallock and Schlager 1986, Granelli et al. 1990, Valiela 1995, Hemminga and Duarte 2000). Direct chemical testing to establish pollution levels can be difficult because of sharp pollution gradients, …

Body Size Of The Endosymbiotic Pea Crab Tumidotheres Maculatus: Larger Hosts Hold Larger Crabs, Karen Kane, Gregory S. Farley

Gulf and Caribbean Research

The endosymbiotic pea crab, Tumidotheres maculatus, uses a broad range of host taxa, including several bivalve species, in the northern Gulf of Mexico. Because shelter size affects the size of other, free-living crab species, we hypothesized that pea crabs living in larger bivalve hosts should attain larger sizes. Crabs and hosts collected from 3 field sites in northern Florida show this trend. We examined crabs living in a large host, the pen shell Atrina rigida, and found them to be larger than pea crabs living in a small host, the bay scallop Argopecten irradians. Moreover, this trend …

Marionia Tedi Ev. Marcus, 1983 (Nudibranchia, Tritoniidae) In The Gulf Of Mexico: First Record Of An Opisthobranch Mollusk From Hydrocarbon Cold Seeps, Angel Valdes

Gulf and Caribbean Research

Cold seeps in the Gulf of Mexico contain relatively diverse molluscan assemblages primarily composed of species that support chemoautotrophic symbionts, such as vesicomyid and mytilid bivalves, but also numerous species of shelled gastropods, bivalves, monoplacophorans, and polyplacophorans (Cordes 2004).

Recent exploration of hydrocarbon seep sites in the Mississippi Canyon and the Vioska Knoll revealed the presence of an unidentified species of nudibranch. The present paper describes the single specimen collected, which constitutes the first published record of an opisthobranch mollusk from a cold seep. The material examined is deposited at the Field Museum of Natural History (FMNH).

New Records For Cubanocuma Gutzui Băcescu And Muradian, 1977 (Crustacea: Cumacea: Nannastacidae) From The Western Atlantic, Richard W. Heard, Joel W. Martin, Thomas J. Hansknecht, Donald B. Cadien

Gulf and Caribbean Research

Because the original description and many subsequent records for Cubanocuma gutzui have appeared in a Romanian journal, Travaux du Muséum d’Histoire naturelle “Grigore Antipa,” which has limited distribution in the west, many workers studying tropical western Atlantic crustaceans still remain unaware of both the genus and species. Also, the species may have gone unrecognized (recorded as an odd specimen of Campylaspis, for instance) or may have been overlooked because of its small size. As part of an ongoing survey of the marine invertebrates of Guana Island, British Virgin Islands (BVI) (led by T.L. Zimmerman and J.W. Martin), numerous specimens …

Baseline Mapping Of Phragmites Australis (Common Reed) In Three Coastal Mississippi Estuarine Basins, Mark S. Peterson, Melissa L. Partyka

Faculty Publications

Over the last two decades, the northern Gulf of Mexico has undergone tremendous growth and development that has resulted in extensive and ongoing habitat modification. We had the opportunity to survey the main channels and bayous of three coastal estuarine basins for the presence and coverage of the invasive Phragmites australis (common reed). The occurrence and area of P. australis was highly variable among the lower Pascagoula River, Back Bay of Biloxi, and St. Louis Bay basins, with the largest amount of coverage (0.489 km(2)) found within the lower Pascagoula River basin and the smallest in Back Bay of Biloxi …

Parallelization Of Multicategory Support Vector Machines (Pmc- Svm) For Classifying Microarray Data, Chaoyang Zhang, Peng Li, Arun Rajendran, Youping Deng, Dequan Chen

Faculty Publications

Background: Multicategory Support Vector Machines (MC-SVM) are powerful classification systems with excellent performance in a variety of data classification problems. Since the process of generating models in traditional multicategory support vector machines for large datasets is very computationally intensive, there is a need to improve the performance using high performance computing techniques.

Results: In this paper, Parallel Multicategory Support Vector Machines (PMC-SVM) have been developed based on the sequential minimum optimization-type decomposition method for support vector machines (SMO-SVM). It was implemented in parallel using MPI and C++ libraries and executed on both shared memory supercomputer and Linux cluster …

Identification Of New Members Of Hydrophobin Family Using Primary Structure Analysis, Kuan Yang, Youping Deng, Chaoyang Zhang, Mohamed O. Elasri

Faculty Publications

Background

Hydrophobins are fungal proteins that can turn into amphipathic membranes at hydrophilic/hydrophobic interfaces by self-assembly. The assemblages by Class I hydrophobins are extremely stable and possess the remarkable ability to change the polarity of the surface. One of its most important industrial applications is its usage as paint. Without detailed knowledge of the 3D structure and self-assembly principles of hydrophobins, it is difficult to make significant progress in furthering its research.

Results

In order to provide useful information to hydrophobin researchers, we analyzed primary structure of hydrophobins to gain more insight about these proteins. In this paper, we presented …

Nomenclatural Innovations In Neotropical Salicaeeae, Mac H. Alford

Faculty Publications

Macrothumia M. H. Alford is described as a new genus of Salicaceae based on Neosprucea kuhlmannii Steamer from Brazil. The deciduous perianth, papillate leaf teeth, presence of receptacular disk glands, and analyses of plastid DNA data Suggest that the new genus is more closely related to Ahernia Merrill and Pleuranthodendron. L. O. Williams than to Banara Aublet or Neosprucea Sleuruer. The monotypic genus Aphaerema Miers of Brazil and Argentina is transferred to Abatia Ruiz & Pavon, based on analyses of plastid DNA sequences and on the lack of morphological discontinuities between the genera. Because the name Abatia spicata (Turczaninow) …

Avian Hosts For West Nile Virus In St. Tammany Parish, Louisiana, 2002, Nicholas Komar, Nicholas A. Panella, Stanley A. Langevin, Aaron C. Brault, Manuel Amador, Eric Edwards, Jennifer C. Owen

Faculty Publications

West Nile virus (WNV) infections in free-ranging birds were studied in Slidell, St. Tammany Parish, Louisiana, after a human encephalitis outbreak peaked there in July 2002. Seroprevalence in resident, free-ranging wild birds in one suburban site was 25% and 24% in August and October, respectively, indicating that most transmission had ceased by early August. Mortality rates, seroprevalence rates, host competence, and crude population estimates were used in mathematical models to predict actual infection rates, population impacts, and importance as amplifying hosts for several common passerine birds. Northern cardinal (Cardinalis cardinalis) and house sparrow (Passer domesticus) were the principal amplifying hosts, …

A Numerical Study Of The Western Cosmonaut Polynya In A Coupled Ocean-Sea Ice Model, T.G. Prasad, Julie L. Mcclean, Elizabeth C. Hunke, Albert J. Semtner, Detelina Ivanova

Faculty Publications

[1] Employing results from a 0.4 degrees, 40-level fully global, coupled ocean - sea ice model, we investigated the role of physical processes emanating from atmosphere, ocean, and ice in the initiation, maintenance, and termination of a sensible heat polynya with a focus on the western Cosmonaut polynya that occurred during May - July 1999. The Cosmonaut polynya first appeared in early May 1999 in the form of an ice-free embayment, transformed into an enclosed polynya on 5 - 9 July, and disappeared by late July, when the ice from the surrounding regions began to encircle the embayment. Except for …

Distribution Of Hydrogen Peroxide In The Northwest Pacific Ocean, Jinchun Yuan, Alan M. Shiller

Faculty Publications

[1] Hydrogen peroxide (H2O2) is a reactive oxygen intermediate involved in the cycling of metals and dissolved organic matter. Because little is known of its distribution in the North Pacific Ocean, we determined H2O2 in surface waters continuously and obtained vertical profiles at nine stations during a cruise from Japan to Hawaii. Surface water H2O2 varied from less than 10 to more than 250 nmol dm(-3). A diel cycle in surface water H2O2 (similar to 25 nmol dm(-3)) was observed only on one day during the monthlong cruise. This is contrary to expectations based on the usual assumption of photo-production …

Soy Isoflavone Glycitein Protects Against Beta Amyloid-Induced Toxicity And Oxidative Stress In Transgenic Caenorhabditis Elegans, Astrid Gutierrez-Zepeda, Ross Santell, Zhixin Wu, Marishka Brown, Yanjue Wu, Ikhlas Khan, Christopher D. Link, Baolu Zhao, Yuan Luo

Faculty Publications

Background

Epidemiological studies have associated estrogen replacement therapy with a lower risk of developing Alzheimer's disease, but a higher risk of developing breast cancer and certain cardiovascular disorders. The neuroprotective effect of estrogen prompted us to determine potential therapeutic impact of soy-derived estrogenic compounds. Transgenic C. elegans, that express human beta amyloid (Aβ), were fed with soy derived isoflavones genistein, daidzein and glycitein (100 μg/ml) and then examined for Aβ-induced paralysis and the levels of reactive oxygen species.

Results

Among the three compounds tested, only glycitein alleviated Aβ expression-induced paralysis in the transgenic C. elegans. This activity of …

Bioluminescence Intensity Modeling And Sampling Strategy Optimization, I. Shulman, D.J. Mcgillicuddy Jr., M.A. Moline, S.H.D. Haddock, J.C. Kindle, D. Nechaev

Faculty Publications

The focus of this paper is on the development of methodology for short-term (1-3 days) oceanic bioluminescence (BL) predictions and the optimization of spatial and temporal bioluminescence sampling strategies. The approach is based on predictions of bioluminescence with an advection-diffusion-reaction (tracer) model with velocities and diffusivities from a circulation model. In previous research, it was shown that short-term changes in some of the salient features in coastal bioluminescence can be explained and predicted by using this approach. At the same time, it was demonstrated that optimization of bioluminescence sampling prior to the forecast is critical for successful short-term BL predictions …

Neotypification Of Lulworthia Fucicola, Jinx Campbell

Faculty Publications

There are no herbarium specimens or culture material for the type of Lulworthia fucicola G.K. Sutherl. With the absence of original material, and to preserve current usage of the name, a neotype is designated hereby. The neotype chosen for L. fucicola is a specimen from Chile.

A Study Of Wave Effects On Wind Stress Over The Ocean In A Fetch-Limited Case, Jiayi Pan, David W. Wang, Paul A. Hwang

Faculty Publications

A field experiment was conducted for collecting three-dimensional wind, wave, and air-sea environmental data off the coast of Louisiana in the Gulf of Mexico in the mixed sea state of a wind sea limited by fetch and with swell propagating against the wind direction. Two methods, the inertial dissipation (ID) and eddy correlation (EC), are used to calculate wind stress. The results show that under the fetch-limited condition, the EC wind stress is greater than the ID stress. This difference is found to be correlated with the counter-wind swell. The swell-related drag coefficient is computed from the wind stress difference …

A Regional Food-Frequency Questionnaire For The Us Mississippi Delta, Katherine L. Tucker, Janice Maras, Catherine Champagne, Carol L. Connell, Susan Goolsby, Judith Weber, Sahar Zaghloul, Teresa Carithers, Margaret L. Bogle

Faculty Publications

Objective: To describe food sources of nutrient intake for white and African American adults in the Lower Mississippi Delta (LMD), and their use in the development of a regional food-frequency questionnaire (FFQ) based on an earlier version of the National Cancer Institute's Health Habits and History Questionnaire. Design: We ranked food sources of energy, macronutrients, vitamins and minerals, and examined portion size distributions for 842 white and 857 African American residents aged 19 years and older, using 24-hour dietary intake recall data from a telephone survey of 36 LMD counties. These values were used to develop a regional FFQ, which …

Recent Observations Of The Whale Shark (Rhincodon Typus) In The Northcentral Gulf Of Mexico, Eric R. Hoffmayer, James S. Franks, John P. Shelley

Gulf and Caribbean Research

The whale shark (Rhincodon typus Smith, 1828) is the world’s largest fish, reaching 15 meters (m) and 18 metric tons (Colman 1997) and is found in all tropical and warm temperate seas (Compagno 2001). The whale shark is listed as ‘vulnerable’ by the International Union for the Conservation of Nature and Natural Resources (IUCN 2004) and is included in Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES 2004). Little is known about whale sharks in the Gulf of Mexico (Gulf). Only reports on the occurrence of whale sharks off Texas …

Ecology Of The Rock Shrimp Sicyonia Dorsalis Kingsley, 1878 (Crustacea: Sicyoniidae) In A Subtropical Region Of Brazil, Rogerio Caetano Da Costa, Adilson Fransozo, Maria Lucia Negreiros-Fransozo

Gulf and Caribbean Research

The present study analyzes the abundance and distribution of the rock shrimp Sicyonia dorsalis, in relation to water temperature, salinity, depth, organic matter content, and sediment texture in Mar Virado (MV), Ubatuba (UBA) and Ubatumirim (UBM), 3 distinct bays along the northern coast of São Paulo State (23°S, 45°W), Brazil. Six transects were taken in each bay, 4 being parallel to the coastline and 2 next to the rocky shores. Monthly samples were taken over a 2-year period (1998 and 1999) with a shrimp fishing boat equipped with double-rig nets. A total of 2,498 specimens was obtained with 804 …

Fishery And Biology Of Blackfin Tuna Thunnus Atlanticus Off Northeastern Brazil, Katia M.F. Freire, Rosangela Lessa, Jorge Eduardo Lins-Oliveira

Gulf and Caribbean Research

Blackfin tuna, Thunnus atlanticus, is the target species of a handline artisanal fishery off northeastern Brazil in September–January, but it is also caught by anglers and as by-catch in industrial fisheries. The population structure, morphometric relationships, mortality, reproduction, and fishery dynamics were studied during 2 fishing seasons (1996 and 1997). The maximum length and weight observed were 87 cm FL and 10 kg W_d, respectively. Males were larger and predominant (1.9:1). The length at 50% maturity was 49.8 cm FL for females and 52.1 cm FL for males. This species uses the area for reproduction, although a …

Coastal Origin Of Common Snook, Centropomus Undecimalis, In Florida Bay, Heather M. Patterson, Ronald G. Taylor, Richard S. Mcbride

Gulf and Caribbean Research

We used the elemental signatures of otoliths to investigate the coastal origin of common snook (Centropomus undecimalis) in Florida Bay, Florida and evaluate current management boundaries. We examined juvenile otoliths from Florida’s Atlantic and Gulf of Mexico (Gulf) populations and determined that there were significant differences in several elemental ratios (Mn/Ca, Cu/Ca, Sr/Ca, Ba/Ca). In addition, a discriminant function analysis (DFA) indicated a significant separation between the juveniles from each coast and otoliths were never misclassified by coast, indicating a distinct difference in their otolith chemistry. Using only juvenile otoliths to derive a calibration function, a separate DFA …

Parasites Of The American White Pelican, Robin M. Overstreet, Stephen S. Curran

Gulf and Caribbean Research

Metazoan symbionts, including parasites, infecting the American white pelican (AWP) Pelecanus erythrorhynchos comprise a list of 75 species, 7 of which are new host records. Several new geographic records are also presented, but generally these have a low value because of the migratory nature of the bird. Evidence suggests that some parasites, mostly flies and other arthropods but also nematodes and digeneans, produce detrimental behavioral or pathologic changes in the AWP. Some of the arthropods transmit microbial agents to the pelican. Two digeneans that have the AWP as a definitive host harm and even kill their catfish intermediate host, especially …

Distribution Of Myodocopid Ostracods In Tampa Bay, Florida, And Association With Abiotic Variables, Stephen A. Grabe

Gulf and Caribbean Research

Myodocopid ostracods were identified from > 600 benthic samples collected from Tampa Bay, Florida, during 1995 to 2001, as part of an annual synoptic survey of the benthos. At least 24 taxa were present. Parasterope pollex was the most abundant (76%) and most frequently collected (48%) species; Rutiderma darbyi (28%) and Eusarsiella disparalis (16%) were the next most frequently collected species. Logistic regression and “center of abundance” calculations were used to identify habitat “preferences” for the most frequently occurring species. With the exception of P. pollex, these were more likely to occur in coarser sediments, in more saline waters, and at …

High Cyanobacterial Abundance In Three Northeastern Gulf Of Mexico Estuaries, Michael C. Murrell, Jane M. Caffrey

Gulf and Caribbean Research

Aquatic phytoplankton comprise a wide variety of taxa spanning more than 2 orders of magnitude in size, yet studies of estuarine phytoplankton often overlook the picoplankton, particularly chroococcoid cyanobacteria (cf. Synechococcus). Three Gulf of Mexico estuaries (Apalachicola Bay, FL; Pensacola Bay, FL; Weeks Bay, AL) were sampled during summer and fall 2001 to quantify cyanobacterial abundance, to examine how cyanobacterial abundance varied with hydrographic and nutrient distributions, and to estimate the contribution of cyanobacteria to the bulk phytoplankton community. Cyanobacterial abundances in all 3 estuaries were high, averaging 0.59 ± 0.76 X 10⁹ L^–1 in Apalachicola Bay, …

First Report Of The Mayan Cichlid, Cichlasoma Urophthalmus (Günther 1862) Collected In The Southern Littoral Zone Of Lake Okeechobee, Florida, Wilfredo A. Matamoros, Keith D. Chin, Bruce Sharfstein

Gulf and Caribbean Research

Here we report the collection of five juvenile Mayan cichlids in Lake Okeechobee, a large, shallow subtropical lake (26°60'N, 80°50'W). These specimens were collected on 4 and 10 November 2003 in the southern littoral zone of Lake Okeechobee at a site off the southwest tip of Torry Island (26°42'N, 80°44'W). The lake stage of this densely vegetated area is highly variable. Nico (in press) reported a single collection of 16 juvenile Mayan cichlids in 2001 from a backwater area in the rim-canal along the NE portion of Lake Okeechobee; an area that is hydrologically isolated from the lake proper, except …

Trophic Relationships Of Demersal Fishes In The Shrimping Zone Off Alvarado Lagoon, Veracruz, Mexico, Edgar Pelaez-Rodriguez, Jonathan Franco-Lopez, Wilfredo A. Matamoros, Rafael Chavez-Lopez, Nancy J. Brown-Peterson

Gulf and Caribbean Research

The diet of demersal piscivorous fishes captured as bycatch of the commercial shrimping fleet off the Alvarado lagoonal system, Veracruz, Mexico, was studied. Nine collections distributed throughout the nortes (windy), wet, and dry seasons were made from November 1993 to January 1995. Sampling yielded a total of 646 fishes representing 10 families and 14 species, of which 44.9% had empty digestive tracts and were excluded from analysis. Trichiurus lepturus and Synodus foetens were the most abundant demersal predators in the collections. Differences in food consumption of the 7 most abundant predators were observed among the 3 seasons, with the greatest …