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Full-Text Articles in Life Sciences

A Pcr/Sequencing Technique To Identify Species And Genotypes Of Haemonchus From Mixed Trichostrongyle Egg Dna Recovered From Livestock Fecal Samples, Pratiksha Khanal Jan 2018

A Pcr/Sequencing Technique To Identify Species And Genotypes Of Haemonchus From Mixed Trichostrongyle Egg Dna Recovered From Livestock Fecal Samples, Pratiksha Khanal

Electronic Theses and Dissertations

Six primer pairs, each with a pair of genus-specific and universal second internal transcribed spacer (ITS2) primers were evaluated to amplify and sequence the ITS2 region of nuclear ribosomal DNA to identify the species and genotypes of Haemonchus from livestock fecal samples containing mixtures of trichostrongyle nematode eggs. Based upon the use of universal ITS2 primers on Haemonchus adults, the ITS2 region has been shown to contain three fixed single-nucleotide differences between H. placei and H. contortus. It is clinically useful to identify the species and genotypes of Haemonchus infecting livestock, but it has not been possible in fecal …

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Evaluation Of Carinata Meal In Dairy Heifer Feeding Programs, Karla Rodriguez-Hernandez Jan 2018

Evaluation Of Carinata Meal In Dairy Heifer Feeding Programs, Karla Rodriguez-Hernandez

Electronic Theses and Dissertations

The main purpose of this body of research was to evaluate carinata meal as a feedstuff for dairy heifers and lay the foundations for its future evaluation in lactating dairy cow diets. This research focused on evaluating the taste preference of carinata meal compared with other oilseeds meals and distillers dried grains with solubles (DDGS). Despite containing glucosinolates, carinata meal was preferred similarly to canola meal, had greater preference compared to camelina meal, and less preference compared to the other meals. Next, we used a limit-feeding strategy in two different feeding studies, to determine the effect of feeding carinata meal …

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Modeling Soil Organic Carbon In Select Soils Of Southeastern South Dakota, Shaina Westhoff Jan 2018

Modeling Soil Organic Carbon In Select Soils Of Southeastern South Dakota, Shaina Westhoff

Electronic Theses and Dissertations

Soil organic matter (SOM) is composed of living biomass, dead plant and animal residues, and humus. Humus is a class of complex, organic molecules that are largely responsible for improving soil water holding capacity, nutrient mineralization, nutrient storage, and other critical soil functions. Soil organic carbon (SOC) accounts for approximately 60 percent of SOM and thus SOC is recognized as a strong indicator of soil health. Land use changes and intense cultivation of arable soils in the United States over the past century have led to large decreases in SOM. The objective of this research was to develop a multiple …

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Evaluation Of Methods Used To Improve Grasslands As Ring-Necked Pheasant (Phasianus Colchicus) Brood Habitat, Mandy Orth Jan 2018

Evaluation Of Methods Used To Improve Grasslands As Ring-Necked Pheasant (Phasianus Colchicus) Brood Habitat, Mandy Orth

Electronic Theses and Dissertations

Management practices designed for upland game species often focus on nest survival and hen winter survival due to the importance of these life history stages on population vital rates. However, chick survival is an important component of gallinaceous bird population dynamics, but it is poorly understood and often tends to be overlooked. Ideal brood habitat not only provides open understory for easy movement and canopy cover for protection, but also provides an abundance of arthropod foods for chicks. It has been hypothesized that restricted movement of chicks through thick vegetation in unmanaged grasslands results in lower brood survival rates. Research …

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Study Of Seasonal Change And Water Stress Condition In Plant Leaf Using Polarimetric Lidar Measurement, Prabeen Kattel Jan 2018

Study Of Seasonal Change And Water Stress Condition In Plant Leaf Using Polarimetric Lidar Measurement, Prabeen Kattel

Electronic Theses and Dissertations

Study of vegetation is of great importance to the improvement of agriculture and forest management. Although there have been various attempts to characterize vegetation using remote sensing techniques, polarimetric lidar is a novel remote sensing tool that has shown potential in vegetation remote sensing. In this thesis, a near-infrared polarimetric lidar at 1064 nm was used to investigate the effects of seasonal change and water stress condition on plant leaves. Two variables, time and water content, affected the plant leaf laser depolarization ratio measurement. The first study focused on the maple tree in order to figure out how seasonal change …

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Nutritional Value Of Modified Novel Oilseed Meals In Teleost Diets, Tom Kasiga Jan 2018

Nutritional Value Of Modified Novel Oilseed Meals In Teleost Diets, Tom Kasiga

Electronic Theses and Dissertations

Carinata Brassica carinata and camelina Camelina sativa are being genetically modified to improve their composition for biofuel production and other agricultural applications. After oil extraction, the residual de-oiled meals (40%) in protein. However, their inclusion in animal feeds is limited to 10% because of high levels of antinutritional factors (ANFs), mainly glucosinolates (GLS), sinapine, and crude fiber that limit diet intake, nutrient utilization, and lower thyroxine production, resulting in reduced growth. The nutritional value of carinata and camelina seed meals to fish were assessed by subjecting cold pressed (CP) carinata and camelina meals to extrusion (EX), solvent extraction (SE) and …

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The Role Of Programs And Policies In Shaping The Observed School Nutrition Environment And Rural Childhood Obesity, Shadai Martin Jan 2018

The Role Of Programs And Policies In Shaping The Observed School Nutrition Environment And Rural Childhood Obesity, Shadai Martin

Electronic Theses and Dissertations

Childhood obesity is an ongoing public health concern that impacts many children in the United States. Research has shown that the prevalence of childhood obesity is higher in rural populations compared to non-rural populations. This may be attributable to differences in the school nutrition environment, particularly school meals and school wellness policy implementation. In order to design effective intervention targeted at weight management and obesity reduction, information regarding the school nutrition environment must be known. Therefore, the purpose of this research was to examine the school nutrition environment, particularly school lunch participation and weight outcomes between metro, non-metro and rural …

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Air-Propelled Organic Fertilizer Grits Can Be Used To Control Weeds And Provide Nitrogen, Michael Carlson Jan 2018

Air-Propelled Organic Fertilizer Grits Can Be Used To Control Weeds And Provide Nitrogen, Michael Carlson

Electronic Theses and Dissertations

Weeds are one of the biggest challenges for organic growers because of the alternative weed control methods. Air-propelled abrasive grit management has been reported to control weed seedlings in corn and soybeans while maintaining yield. This research examined the weed control, corn and soybean yields, nitrogen mineralization, and nitrogen yield responses from grits. The grits used in this research included: Phytaboost Plant Food 7-1-2 (soybean meal), Sustane 8-2-4 and 4-6-4 (turkey litters), and two non-fertilizer grits: Agra Grit (walnut shells) and corn cob meal. Field studies were conducted from 2015 to 2017 in Aurora, SD, Beresford, SD and Morris, MN. …

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Effects Of Enzymatically Hydrolyzed Yeast Supplementation And Supplementation Frequency On Immune Parameters, Performance, And Digestibility Among Periparturient Beef Cows And Calves, Janine Swartz Jan 2018

Effects Of Enzymatically Hydrolyzed Yeast Supplementation And Supplementation Frequency On Immune Parameters, Performance, And Digestibility Among Periparturient Beef Cows And Calves, Janine Swartz

Electronic Theses and Dissertations

Effects of enzymatically hydrolyzed yeast (EHY) and supplementation frequency (FREQ) on immune parameters among beef cows and calves was evaluated. Eighty multiparous (parity = 4.2 ± 0.3) cows were fed a common brome hay-based diet (CP = 8.0 ± 0.17%). Cows were blocked by expected calving date and stratified by body condition score (BCS) before random assignment of treatment. Beginning 88 ± 5 d prior to parturition, cows were provided 1 kg daily or 3 kg every 3 d of a soybean hull-based supplement (CP = 34.0, % of DM) that contained 0 or 3 g/kg EHY. The daily supplement …

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Nutritional And Health Benefits Enhancement Of Wheat-Based Food Products Using Chickpea And Distiller’S Dried Grains, Waleed H.M. Alrayyes Jan 2018

Nutritional And Health Benefits Enhancement Of Wheat-Based Food Products Using Chickpea And Distiller’S Dried Grains, Waleed H.M. Alrayyes

Electronic Theses and Dissertations

The first objective of this research was to enhance nutritional, rheological, sensory profiles, and shelf life of wheat-based pita bread using chickpea (CP) and food grade distiller’s dried grains (FDDG) as fortification ingredients. CP and FDDG are both high-protein and high-fiber ingredients. Nutritional efficacy was evaluated. Dough rheology and product texture were also analyzed. Chemical, physical, and rheological properties of blends, doughs and finished products were evaluated and the results showed an increase in protein, fat, ash, and total dietary fiber with an increase of FDDG and CP in the wheat-based food formulation. Moisture content was decreased in both flour …

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Proteogenomic Study Of Nitrogen-Fixing Cyanobacterium Anabaena Cylindrica, Yeyan Qiu Jan 2018

Proteogenomic Study Of Nitrogen-Fixing Cyanobacterium Anabaena Cylindrica, Yeyan Qiu

Electronic Theses and Dissertations

Cyanobacteria are gram-negative photosynthetic bacteria. In some filamentous cyanobacteria such as Anabaena cylindrica, every 10th to 20th vegetative cells can differentiate into nitrogen fixing heterocysts. Heterocyst can induce the neighboring cells developing into spore-like akinetes. The specialized cell functions and resourceful networks have contributed to the prosperity of cyanobacteria for over 2 billion years, but the genetic mechanisms for multi-cellular differentiation are barely known, especially for akinete formation. The heterocysts, akinetes and vegetative cells of Anabaena cylindrica were isolated for proteomic study. This study identified a total of 1395 proteins, including 664 proteins from akinetes, 751 proteins from …

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Insecticidal Seed And In-Furrow Treatment Recommendations For Soybean And Sunflower, Brady Hauswedell Jan 2018

Insecticidal Seed And In-Furrow Treatment Recommendations For Soybean And Sunflower, Brady Hauswedell

Electronic Theses and Dissertations

Throughout the 2016 and 2017 growing season, field research experiments were replicated across South Dakota. Many times seed treatments are used prophylactic, which is neither good for the producers or the environment. Producers will be able to reduce production costs, if they only use a seed treatment when necessary. The purpose of the first experiment was to determine the effects of seed treatments in combination with planting date and seeding rate on soybean yield. To determine the effects, two years of field data from four eastern South Dakota locations were compared. Within each year and location we compared two planting …

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Trunk Stability And Postural Stability In People With Multiple Sclerosis, Derek Tolbert Jan 2018

Trunk Stability And Postural Stability In People With Multiple Sclerosis, Derek Tolbert

Electronic Theses and Dissertations

Multiple Sclerosis is a neurological disease which affects an estimated 2.5million people worldwide. People with Multiple Sclerosis often experience high rates of falls, which have been associated with age, disability, and increased postural sway. Additionally, people with Multiple Sclerosis often exhibit muscular weakness and poor responses to perturbations. PURPOSE: To determine if trunk stability and postural control are altered among PwMS and if trunk muscle activity is correlated with postural stability. METHODS: Ten participants with a physician's diagnosis of Multiple Sclerosis (9 female, 1 male) were included in this study. Ten healthy controls were matched for age, height, weight, and …

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Perceptions Of Green Eating Behaviors On College Campuses In An Urban Vs. Rural Setting, Gabriella G. Nowicki Jan 2018

Perceptions Of Green Eating Behaviors On College Campuses In An Urban Vs. Rural Setting, Gabriella G. Nowicki

Electronic Theses and Dissertations

Background: Green Eating is a multidisciplinary approach to health in economic, public health, and environmental issues. GE has an evident impact on the quality of life in young adults, especially those on college campuses. Current evidence already supports an improved dietary intake in those who adopt GE behaviors. Objective: To determine if a university’s campus location affects first-year students’ exposure to green eating. We hypothesize that first-year students on university campuses located in urban settings are more likely to report GE awareness and practices than in rural settings.
Methods: First-year students were recruited for the GetFruved study in late summer …

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Effects Of Standards-Based Grading On Students In Agricultural Education, Matthew Tripp Jan 2018

Effects Of Standards-Based Grading On Students In Agricultural Education, Matthew Tripp

Electronic Theses and Dissertations

Standards-based grading is being discussed more frequently in the field of education as school districts either explore or adopt this grading method. This study examined the effects of standards-based grading on achievement and perceptions of students enrolled in high school agricultural education courses. This nonequivalent quasi-experimental study focused on how students’ perceptions of classroom feedback, abilities, and opportunities to redo assessments were affected by the implementation of a standards-based grading method. Participants in this study were students who enrolled in a quarter long Home Maintenance course that used standards-based grading methods to assess student mastery on specific content standards in …

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Mechanisms By Which Mechanotransduction Promotes Proliferation In Keratinocytes, Chhavi Chaudhary Jan 2018

Mechanisms By Which Mechanotransduction Promotes Proliferation In Keratinocytes, Chhavi Chaudhary

Electronic Theses and Dissertations

Chronic wounds are wounds that do not heal within 30 days and often they can last over a year. Interference in any of the wound healing stages may hinder the process. Some of the local and systemic factors such as infection, old age, diabetes, AIDS, and the regular application of corticoids may also have negative effects on the healing process. Cell proliferation is an important phase in epidermal wound healing in which surviving epithelial cells replicate independently into daughter cells through mitosis and maintain a balance between cell growth and cell loss during the cell cycle. Physical and chemical stimuli …

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Weight Management Related Factors And Healthy Behaviors In Adults, Sumadhuri Pamarthi Jan 2018

Weight Management Related Factors And Healthy Behaviors In Adults, Sumadhuri Pamarthi

Electronic Theses and Dissertations

Obesity is an ongoing global pandemic that affects more than one in three adults in the United States. Being overweight or obese also increases the risk of chronic health conditions. Weight management does not only center on immediate weight loss but also on the proper maintenance of an appropriate body weight over the years. Methods of weight management include eating a healthy diet and meeting physical activity guidelines. Such weight management practices are important to ensure that the people suffering from obesity can decrease risks of morbidity or even mortality. Nevertheless, unhealthy weight management strategies might lead to unintended health …

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Distribution, Ecology, Disease Risk, And Genetic Diversity Of Swift Fox (Vulpes Velox) In The Dakotas, Emily Louise Mitchell Jan 2018

Distribution, Ecology, Disease Risk, And Genetic Diversity Of Swift Fox (Vulpes Velox) In The Dakotas, Emily Louise Mitchell

Electronic Theses and Dissertations

The swift fox (Vulpes velox), a native species once abundant throughout the Northern Great Plains (NGP), has declined due to changes in land use, historic predator eradication programs, and predation by larger canid species. Currently, the species is estimated to occupy 44% of its historic range. In the NGP, the status of the swift fox varies by state, ranging from furbearer to endangered species. However, knowledge of the current status of swift foxes in the NGP is lacking due to an absence of systematic population monitoring. Improving the current state of knowledge concerning swift fox populations in the …

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Evaluating Bighorn Sheep Herd Response After Selective Removal Of Mycoplasma Ovipneumoniae Chronic Shedders, Tyler J. Garwood Jan 2018

Evaluating Bighorn Sheep Herd Response After Selective Removal Of Mycoplasma Ovipneumoniae Chronic Shedders, Tyler J. Garwood

Electronic Theses and Dissertations

Infected individuals vary in their contribution to disease persistence, and chronically infected individuals may sustain disease in a population. One disease that might persist in a population through chronically infected individuals is pneumonia in wild sheep. Mycoplasma ovipneumoniae (Mo), a pathogen of Caprinae commonly present in domestic sheep and goats, strongly correlates with pneumonia epizootics when it infects wild sheep populations. These epizootics can cause 40-100% herd mortality in an initial all-age dieoff, precipitate annual lamb mortality as high as 100% in following years, and sustain adult mortality long after initial all-age dieoffs. We conducted an experiment in the Black …

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Improving The Nutritional Characteristics Of Plant Feedstuff By-Products Using Fungal Metabolism, Jacob Zahler Jan 2018

Improving The Nutritional Characteristics Of Plant Feedstuff By-Products Using Fungal Metabolism, Jacob Zahler

Electronic Theses and Dissertations

Plant feedstuff by-products such as soy processing wastewater, guar korma meal, and sorghum hominy are very different, however, all possess a rich nutrient profile. Lessening their value is the presence of lignocellulose, plant anti-nutritional factors, and unfavorable protein profiles. Fungal conversion processes are an attractive approach to improving the value of these by-products by degrading detrimental fractions of each while simultaneously creating nutrient-rich cell mass. The aim of this research was to improve the value of each by-product for potential application in monogastric diets such as fish. Fungal organisms, both yeast and filamentous fungi, were examined for their ability to …

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A Quantitative Environmental Assessment Of Incorporating Torrefaction Into Farming Enterprises In Eastern South Dakota, Dinesh Fuyal Jan 2018

A Quantitative Environmental Assessment Of Incorporating Torrefaction Into Farming Enterprises In Eastern South Dakota, Dinesh Fuyal

Electronic Theses and Dissertations

The use of renewable energy sources has been increasing in the recent years due to population growth and environmental concerns. Biomass is a promising energy source that can be used to produce biofuels or torrefied pellets. Torrefied biomass may be used in power plants, industrial and residential heating, feedstocks for gasification, air and water filtrating, and soil amendment. The interest of torrefied pellets as energy sources for various applications has been increased in the recent years due to the concerns about energy security and environmental issues. This study focuses on the economic and environmental assessment of agricultural feedstocks like corn …

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Identification Of Macropinocytosis Regulating Proteins And Signaling From Macropinosomes, Louise Monga Jan 2018

Identification Of Macropinocytosis Regulating Proteins And Signaling From Macropinosomes, Louise Monga

Electronic Theses and Dissertations

Understanding macrophage cell biology is important due to macrophages key roles in human health and diseases including proper immune function, wound healing, atherosclerosis, and cancer. Despite their importance, relatively little is understood about macrophage activation, growth factor signaling, and cytoskeletal regulation. This thesis presents data from investigations into mechanisms of macrophage growth factor signaling and actin polymerization for ruffling and macropinocytosis. Alt-R CRISPR-Cas9 method and dextran uptake assay were used to knock out individual genes (SHP-1, Lyn, Syk, BTK, Vav1) and determine their role in macropinocytosis and CSF-1R signaling. Dextran uptake was disrupted in SHP-1 and Lyn targeted knockout cells, …

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Western Prairie Stream Fisheries: An Assessment Of Past And Present Fish Assemblage Structure, Biotic Homogenization, And Population Dynamics In Western South Dakota Streams, Stephen Jones Jan 2018

Western Prairie Stream Fisheries: An Assessment Of Past And Present Fish Assemblage Structure, Biotic Homogenization, And Population Dynamics In Western South Dakota Streams, Stephen Jones

Electronic Theses and Dissertations

Biodiversity is declining globally, especially in in aquatic systems. Prairies are one of the most endangered ecosystem types in North America, and their conversion into agriculture or development as a result of urbanization has had negative effects on prairie stream fish communities. Continued monitoring of fish assemblage change and the population dynamics of prairie stream fishes will provide researchers and managers with valuable information needed to detect and mitigate past and future negative impacts on prairie stream aquatic ecosystems. Sampling was conducted in the mainstem and tributaries of the Grand, Moreau, Cheyenne, Bad, and White rivers within western South Dakota. …

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The Journal Of Undergraduate Research: 2018 Jan 2018

The Journal Of Undergraduate Research: 2018

The Journal of Undergraduate Research

This is the complete issue of the South Dakota State University Journal of Undergraduate Research , Volume 16.

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Measuring The Influence Of Bedding On Soil Nitrogen Losses And Corn Crop Nitrogen Characteristics For Fall Applied Solid Beef Cattle Manure In Eastern South Dakota, Mukesh Mehata Jan 2018

Measuring The Influence Of Bedding On Soil Nitrogen Losses And Corn Crop Nitrogen Characteristics For Fall Applied Solid Beef Cattle Manure In Eastern South Dakota, Mukesh Mehata

Electronic Theses and Dissertations

Nitrogen (N) is a major component of chlorophyll which plays a key role in the photosynthesis process in crops. The N is one of the highest demanded nutrients by all plants for their growth and reproduction. Manure or inorganic fertilizer is often applied to fulfill the crops’ N demand. However, the applied N sources have the potential of N losses in different forms from the soil volume in many ways such as ammonia (NH3) volatilization, aerial nitrous oxide (N2O) loss, nitrate (NO3 --N) leaching, and runoff and/or erosion. Soil fertility, crop yield, water quality, and …

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Transcriptome Analysis Of Root Development In Wheat Triticum Aestivum Using High Throughtput Sequencing Technologies, Ghana Challa Jan 2018

Transcriptome Analysis Of Root Development In Wheat Triticum Aestivum Using High Throughtput Sequencing Technologies, Ghana Challa

Electronic Theses and Dissertations

Root provides plant water, nutrients and anchorage from soil. Most our knowledge of molecular mechanisms of root development is from the dicot model plant Arabidopsis, but very few studies have done in monocot crop systems like rice, maize, and wheat. We are studying very short root (VSR) phenotype in wheat, and lack of a sequenced reference genome in wheat prompted us to sequence and assemble the root transcriptome of the reference cultivar Chinese Spring (CS). A root transcriptome was assembled from the sequenced reads generated from root tip and the mature root tissues of CS. Approximately 169 million reads were …

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Pilot Study: Knowledge And Attitudes Regarding Sport-Related Concussion In A Rural Interscholastic Sport Sample, Austin Westland Jan 2018

Pilot Study: Knowledge And Attitudes Regarding Sport-Related Concussion In A Rural Interscholastic Sport Sample, Austin Westland

Electronic Theses and Dissertations

Context: Evidence supports education initiatives to improve knowledge of sport-related concussion (SRC) in active children and adolescents, as well as improve attitudes towards reporting concussion events to a supervising adult with the ultimate goal of early diagnosis and management. Most evidence focuses on urban and suburban children’s SRC knowledge and attitudes; however, little is known regarding knowledge and attitudes of children participating in sport in rural environments. Understanding current knowledge and attitudes of rural children can inform future education and health behavior strategies that encourage early reporting. Objective: Two objectives guided this study. First, obtain and analyze pilot data regarding …

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Exploring The Relationship Between Quality Of Written School Wellness Policy And The Degree Of Wellness Policy Implementation In Elementary Schools, Josie Sieberg Jan 2018

Exploring The Relationship Between Quality Of Written School Wellness Policy And The Degree Of Wellness Policy Implementation In Elementary Schools, Josie Sieberg

Electronic Theses and Dissertations

Background: To date many studies have evaluated the quality of written school wellness policies (SWPs), however, few have addresses SWP implementation. As SWPs have the potential to reduce childhood obesity, it is crucial for schools to not only write high quality SWPs, but also to implement these policy items. Purpose: The purpose of this study was to assess the relationship between the quality of written SWPs and the degree of SWP implementation. We hypothesized that schools with higher quality written SWPs would have a higher degree of policy implementation. Methods: School wellness policy written quality and implementation were assessed in …

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Development Of Biclustering Techniques For Gene Expression Data Modeling And Mining, Juan Xie Jan 2018

Development Of Biclustering Techniques For Gene Expression Data Modeling And Mining, Juan Xie

Electronic Theses and Dissertations

The next-generation sequencing technologies can generate large-scale biological data with higher resolution, better accuracy, and lower technical variation than the arraybased counterparts. RNA sequencing (RNA-Seq) can generate genome-scale gene expression data in biological samples at a given moment, facilitating a better understanding of cell functions at genetic and cellular levels. The abundance of gene expression datasets provides an opportunity to identify genes with similar expression patterns across multiple conditions, i.e., co-expression gene modules (CEMs). Genomescale identification of CEMs can be modeled and solved by biclustering, a twodimensional data mining technique that allows clustering of rows and columns in a gene …

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Differences In Walking Mechanics Between A Traditional Walker And The Kb Balance Trainer, Silvia Zanini Jan 2018

Differences In Walking Mechanics Between A Traditional Walker And The Kb Balance Trainer, Silvia Zanini

Electronic Theses and Dissertations

INTRODUCTION: Millions of individuals with ambulatory difficulties rely on walking aids to maintain independence and mobility. However, users of traditional walkers typically exhibit increased forward lean of their trunk while using the assistive device. The KB Balance Trainer is a new posterior walker designed to facilitate a more erect position during gait. PURPOSE: To compare gait mechanics across three walking conditions: unassisted, using a traditional walker, and using the KB Balance Trainer. METHODS: Seven adults with experience using walkers due to ambulatory difficulties participated in the study. The study consisted of one training session and one gait analysis session. The …

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