Life Sciences Commons^™

A Reflection Of Experiences Of Adults With Type 1 Diabetes In Integrated Physical Education Classes, Kalleigh West

Human Movement Studies & Special Education Theses & Dissertations

The purpose of this study was to gain an understanding of the experiences of type 1 diabetics in integrated physical education classes. In this study, we interviewed young type 1 diabetic adults and asked them to reflect on their school-based physical education experiences. An interpretative phenomenological analysis (IPA) approach was adopted to guide data collection, analysis, and interpretation for this retrospective study. Eight participants (ages 19 to 32) were enrolled in this study, and semi-structured interviews focused on their physical education experiences acted as the primary data. Transcribed interview data were analyzed using an IPA approach. Three interrelated themes emerged …

Tracing And Segmentation Of Molecular Patterns In 3-Dimensional Cryo-Et/Em Density Maps Through Algorithmic Image Processing And Deep Learning-Based Techniques, Salim Sazzed

Computer Science Theses & Dissertations

Understanding the structures of biological macromolecules is highly important as they are closely associated with cellular functionalities. Comprehending the precise organization of actin filaments is crucial because they form the dynamic cytoskeleton, which offers structural support to cells and connects the cell’s interior with its surroundings. However, determining the precise organization of actin filaments is challenging due to the poor quality of cryo-electron tomography (cryo-ET) images, which suffer from low signal-to-noise (SNR) ratios and the presence of missing wedge, as well as diverse shape characteristics of actin filaments. To address these formidable challenges, the primary component of this dissertation focuses …

Effect Of Acute Caffeine Ingestion On On-Field Performance In Division 1 Female Field Hockey Players, Nicole Danielle Fredricks

Human Movement Studies & Special Education Theses & Dissertations

This study aimed to determine the effects of acute caffeine ingestion on on-field performance in collegiate female field hockey players. A placebo-controlled, double-blind, randomized, crossover experimental design was utilized. The participants (N=10) were female division 1 field hockey players (age: 20.0 ± 1.6 years). They competed in two 60-minute, off-season scrimmages separated by two weeks. They were randomly assigned to ingest the caffeine, 3 mg/kg of body weight, or placebo treatment 60 minutes before each scrimmage. They wore a PLAYERTEK (Catapult, Australia) global positioning system (GPS) device to track their total distance, sprint distances, power plays, and zone three and …

Modified Vaccine Vectors To Understand Adjuvant Functions Of Listeria During Chronic Schistosomiasis, Stephanie K. Norwood

Biological Sciences Theses & Dissertations

Vaccination is one of the most effective strategies employed to prevent infectious diseases. Successful vaccination is dependent upon the induction of a specific, robust, and prolonged immune response. One of the major challenges faced by vaccine development is vaccine failure due to host-related factors that can modulate the immune system, which leads to non-responsiveness to vaccinations. The generation of new vaccine strategies is imperative to combat these effects. Live bacterial vectors are one approach used as they can elicit humoral immunity, cellular immunity, or both. Listeria monocytogenes is a Gram positive, intracellular pathogen that is an effective bacterial vaccine vector …

Dental Hygiene Students Reported Physiological Symptoms Associated With Wearing An N95 Respirator Mask, Peyton Shea Butler

Dental Hygiene Theses & Dissertations

Purpose: Physiological symptoms and comfort levels while wearing an N95 respiratory mask has not been examined with dental hygienists. The purpose of this study was to investigate dental hygiene students reported physiological symptoms and comfort perception while wearing an N95 respirator mask during patient care appointments. Methods: After IRB approval (IRB #1987754-2), a 16-item questionnaire was distributed through email to a convenience sample of 65 dental hygiene students. Questions assessed respiratory, dermatologic, cardiac, mask mouth and general physiological symptoms, as well as comfort levels. Additionally, participants were asked to respond to demographic questions and one open ended question inquiring about …

Age, Lower Extremity Muscle Strength, And Running Biomechanics In Healthy Female Recreational Runners, Heather Mcconchie Hamilton

Rehabilitation Sciences Theses & Dissertations

Middle-age and older runners demonstrate differences in running biomechanics compared to younger runners. Females and males demonstrate differences in running biomechanics, and females experience additional hormonal changes with age due to menopause. Despite the biomechanical and physiological differences between females and males, little research has investigated the effect of age specifically in female runners. The overall purpose of this research was to determine the relationships among age, lower extremity muscle strength, and running biomechanics in healthy female recreational runners. Healthy female recreational runners aged 25 – 65 years were recruited. Participants ran on an instrumented treadmill at a training pace …

The Effects Of Lifting With Intent On Hip And Knee Moments During Back Squats, Kyle A. Parrish

Human Movement Studies & Special Education Theses & Dissertations

Back squatting is a widely used movement in the strength and conditioning community. Within the last few years, velocity-dependent training and its application of tracking intensity have increased in popularity. Biomechanics research has frequently examined force generation, joint loading, and muscle contributions of back squats with different bar placement, stance width, and increasing barbell loads. However, no information exists regarding whether lifting with intent increases velocity and how increased velocity is biomechanically achieved. This study aimed to examine how lifting with intent affects bar velocity and how joint moment alterations could contribute to these potential changes in velocity. Fifteen males …

Structural Insights Into The Cl-Par-4 Protein: Ionic Requirements, Conformational Transitions, And Interaction With Cisplatin, Krishna Kumar Raut

Chemistry & Biochemistry Theses & Dissertations

Cancer continues to be the leading global cause of death, with challenges in early diagnosis, drug resistance, non-specific drug targeting, and cancer recurrence and metastasis posing formidable obstacles in cancer therapy. In this context, Prostate Apoptosis Response-4 (Par-4), a pro-apoptotic tumor suppressor protein, emerged as a promising therapeutic target due to its ability to selectively induce apoptosis in cancer cells, thereby minimizing the drug-associated adverse effects. However, a comprehensive understanding of the structural features of Par-4, specifically the caspase-cleaved fragment (cl-Par-4), is crucial for therapeutic advancements.

This dissertation investigated the effects of various ions, both monovalent and divalent, on the …

Effects Of A Climbing-Specific Training Program On Upper-Body Power In Novice Climbers Compared To Advanced Climbers, Dallas E. Wood

Human Movement Studies & Special Education Theses & Dissertations

Muscular strength and muscular endurance are integral physical components of rock climbing. The hypothesis of this study was that a climbing-specific training program would improve physical fitness specific to climbing and improve performance on a relevant indoor rock-climbing test in novice climbers.

Twenty-one novice recreational climbers were matched for sex and climbing performance and randomly assigned. The experimental (EXP, n = 11) group was provided a climbing-specific, six-week training program. The control (CON, n = 10) group continued training as usual. Pre- and post-tests involved anthropometric tests, the ape-index test (arm span to height ratio), a weighted pull-up test, a …

Biomechanical Adaptations While Performing Bilateral Drop Landings With A Unilateral Ankle Tape Application, Eric Daniel Jenkins

Rehabilitation Sciences Theses & Dissertations

Ankle sprains are the most common injury in sport and exercise performance, which makes the utilization of ankle taping a common procedure to both prevent potential sprain, as well as protect against reinjury. However, unilateral ankle taping may have unintended consequences on the mechanics of the ankle and other joints of both legs. The aim of this dissertation was to determine the effects of ankle taping on lower body kinetics and kinematics, stiffness, and coordination during a bilateral landing task.

Twelve female participants completed a total of 90 drop landings across two visits, randomized from landing platforms of 30, 45, …

Pulsed Electric Field Ablation: Mechanisms Of Differential Cell Sensitivity And Methods To Mitigate Neuromuscular Excitation, Emily Gudvangen

Biomedical Engineering Theses & Dissertations

Ablation therapies aim to destroy tumors while maintaining minimal damage to surrounding healthy tissue. Pulsed electric field (PEF) ablation targets the cell membrane during the destruction of tissues, which consequently spares extracellular structures such as ducts and blood vessels, leaving the framework for tissue regeneration. However, the utility of PEF is limited by neuromuscular excitation, causing pain and involuntary muscle contraction. To identify PEF protocols that efficiently ablate tissue while minimizing neuromuscular stimulation, various PEF protocols differing in pulse duration, shape, and repetition rate were tested. The ratio of cell death and nerve excitation thresholds, which determines the range of …

Monarch Science Observer, Volume 17, College Of Sciences, Old Dominion University

College of Sciences Newsletter

Fall 2023, issue of Monarch Science Observer, ODU Colleges of Sciences Newsletter.

Effects Of Chewing Time On Gastrointestinal Discomfort, Substrate Use, And Performance During Running, Thomas Geaney

Human Movement Studies & Special Education Theses & Dissertations

Previous research has shown that food particle size affects gastric processing. For example, food particles greater than 3 mm may delay gastric emptying under certain conditions. Delays in gastric emptying can be problematic during aerobic exercise, leading to nausea, bloating, fullness, and other gastrointestinal (GI) symptoms. In some cases, symptoms can be severe enough to negatively affect athletic performance. This study investigated the effects of manipulating chewing duration of carbohydrate sports bars on GI discomfort, physiological responses, and performance during endurance running. This crossover study recruited 12 male runners (age: 36.4 ± 7.2 years, VO_2peak: 57.2 ± 4.7 …

Affect, Mind-Body Factors, And Disordered And Intuitive Eating Behaviors: Examining Naturalistic Associations Among Young Women With Elevated Eating Disorder Symptoms, Kelly A. Romano

Psychology Theses & Dissertations

Objective: The present study examined whether three mind-body factors—emotion dysregulation, interoceptive sensibility, and mindfulness—that are theorized to be implicated in the onset and maintenance of eating disorder (ED) pathology mediated (Aim 1) and moderated (Aim 2) within-person associations between affect and women with elevated ED symptoms’ disordered and intuitive eating behavior use. Method: Participants included 150 young women with elevated ED symptoms who completed a 10-day ecological momentary assessment protocol. To address Aim 1, multilevel structural equation models examined whether the mindbody factors (separately) mediated momentary associations between negative and positive affect, and women’s disordered (dietary restriction, loss of control …

Age-Related Differences In Motor Performance, Jessica Anne Prebor

Rehabilitation Sciences Theses & Dissertations

The purpose of this work was to study the age effects on average performance and variability of movement responses in children, young adults, and older adults across multiple motor tasks. Optimal motor performance is observed in healthy young adults with declines observed at either end of the lifespan. This pattern has been represented as a U-shaped/inverted U-shaped curve. Little is known about if this pattern persists in chewing dynamics. While chewing has been found to improve aspects of attention, a cognitive function, research is limited on the relationship between chewing and other motor tasks.

The first aim of this research …

Tidal Flooding In The Mid-Atlantic Region Of The Us: Water Quality Effects In The Lower Chesapeake Bay, Alfonso Macias Tapia

OES Theses and Dissertations

Many coastal areas around the globe suffer from nutrient pollution and its environmental, social, and economic consequences. Nutrient inputs can come from point (e.g., the end of a pipe) and nonpoint sources, from which the former are better constrained as sampling need only be conducted at a discharge point. Given the temporal and spatially extensive nature of tidal flooding events, they can represent another type of nonpoint source of nutrients to adjacent water bodies heretofore, unexamined and quantified. Most studies examining impacts of tidal flooding have focused on threats to resources on land, such as urban infrastructure and human health …

Influence Of Abiotic Drivers And Plant Community Interactions On Bald Cypress (Taxodium Distichum) Seedlings: Implications For Restoration, Victoria Ellis

Biological Sciences Theses & Dissertations

Forested freshwater wetlands are valuable ecosystems that provide habitat for numerous species, sequester carbon, and act as sinks for excess water and nutrients. Historically, these ecosystems have been heavily degraded by anthropogenic activities leading to loss of ecosystem services and a desire to restore freshwater forested wetland habitat. Thus, science-backed approaches for the restoration of freshwater forested wetlands are necessary to ensure restoration goals are met. This body of research employed the Stress Gradient Hypothesis to test whether a multi-species planting approach using Juncus effusus (L.) (soft rush) could facilitate the survival of Taxodium distichum (L.) Rich. (bald cypress) seedlings …

Effects Of Physical Characteristics Of Placebos On Exercise Performance, Owen Sipes

Human Movement Studies & Special Education Theses & Dissertations

Previous research has shown that placebos can impact medical conditions such as irritable bowel syndrome and depression as well as elicit analgesic effects and improvements in certain exercise performances. These placebo responses occur from a variety of alterations to the placebo itself or through verbal suggestion of how helpful the placebo will be. This study aimed to observe whether changing the color and quantity of capsules can induce improvements in both vertical jump and hand grip strength. This deceptive, parallel study consisted of 28 participants, with a mean age of 24.9 (±4.3) years old, that were randomly assigned to receive …

A Dynamical Systems Approach To Characterizing Brain–Body Interactions During Movement: Challenges, Interpretations, And Recommendations, Derek C. Monroe, Nathaniel T. Berry, Peter C. Fino, Christopher K. Rhea

Rehabilitation Sciences Faculty Publications

Brain–body interactions (BBIs) have been the focus of intense scrutiny since the inception of the scientific method, playing a foundational role in the earliest debates over the philosophy of science. Contemporary investigations of BBIs to elucidate the neural principles of motor control have benefited from advances in neuroimaging, device engineering, and signal processing. However, these studies generally suffer from two major limitations. First, they rely on interpretations of ‘brain’ activity that are behavioral in nature, rather than neuroanatomical or biophysical. Second, they employ methodological approaches that are inconsistent with a dynamical systems approach to neuromotor control. These limitations represent a …

Control Of The Electroporation Efficiency Of Nanosecond Pulses By Swinging The Electric Field Vector Direction, Vitalii Kim, Iurii Semenov, Allen S. Kiester, Mark A. Keppler, Bennett L. Ibey, Joel N. Bixler, Ruben M. L. Colunga Biancatelli, Andrei G. Pakhomov

Bioelectrics Publications

Reversing the pulse polarity, i.e., changing the electric field direction by 180°, inhibits electroporation and electrostimulation by nanosecond electric pulses (nsEPs). This feature, known as “bipolar cancellation,” enables selective remote targeting with nsEPs and reduces the neuromuscular side effects of ablation therapies. We analyzed the biophysical mechanisms and measured how cancellation weakens and is replaced by facilitation when nsEPs are applied from different directions at angles from 0 to 180°. Monolayers of endothelial cells were electroporated by a train of five pulses (600 ns) or five paired pulses (600 + 600 ns) applied at 1 Hz or 833 kHz. Reversing …

Metals And Metal Complexes In Diseases With A Focus On Covid-19: Facts And Opinions, Agnieszka Ścibior, Manuel Aureliano, Alvin A. Holder, Juan Llopis

Chemistry & Biochemistry Faculty Publications

In the present Special Issue on “Metals and Metal Complexes in Diseases with a Focus on COVID-19: Facts and Opinions”, an attempt has been made to include reports updating our knowledge of elements considered to be potential candidates for therapeutic applications and certain metal-containing species, which are extensively being examined towards their potential biomedical use due to their specific physicochemical properties. The Special Issue compiles data on the role of metals in COVID-19 and focuses on other illnesses and biological processes that affect metal metabolism. It consists of eight manuscripts, including five review articles and three original research papers (Figure …

Newer Surveillance Data Extends Our Understanding Of The Niche Of Rickettsia Montanensis (Rickettsiales: Rickettsiaceae) Infection Of The American Dog Tick (Acari: Ixodidae) In The United States, Catherine A. Lippi, Holly D. Gaff, Robyn M. Nadolny, Sadie J. Ryan

Biological Sciences Faculty Publications

Background: Understanding the geographic distribution of Rickettsia montanensis infections in Dermacentor variabilis is important for tick-borne disease management in the United States, as both a tick-borne agent of interest and a potential confounder in surveillance of other rickettsial diseases. Two previous studies modeled niche suitability for D. variabilis with and without R. montanensis, from 2002-2012, indicating that the D. variabilis niche overestimates the infected niche. This study updates these, adding data since 2012.

Methods: Newer surveillance and testing data were used to update Species Distribution Models (SDMs) of D. variabilis, and R. montanensis infected D. variabilis, in …

Opensim-Based Musculoskeletal Modeling: Foundation For Interactive Obstetric Simulator, Bahador Dodge

Electrical & Computer Engineering Theses & Dissertations

The use of mathematical and computational models to understand complex biological systems, such as the human birth process, is a rapidly growing field in medicine. These models can be used to optimize and personalize medical treatments for individual patients, enhance training, and aid in educational efforts. While recent advancements in healthcare, particularly in obstetrics, have improved care for mothers and babies, studies and government reports indicate a rising rate of maternal mortality in the United States.

Despite this rising trend, there is a lack of detailed studies concerning the use of modeling and simulation to develop an interactive obstetrics simulator …

Nanosecond Pulsed Electric Field Modulates Electron Transport And Mitochondrial Structure And Function, Lucas Nelson Potter

Biomedical Engineering Theses & Dissertations

Pulsed power treatment has been used to induce regulated cell death (RCD) in cells or ablate tumors in animals. A subset of pulsed power as electroporation with high voltage and pulse duration of milliseconds is used for biomedical treatment to induce pores in the plasma membrane of cells. Nanosecond Pulsed Electric Fields (nsPEFs)– an extension of electroporation, uses waveforms with pulse durations on the order of 10-900 nanoseconds. nsPEF treatment has demonstrated intracellular effects for potential biomedical applications. In this work, nsPEF treatment is used to demonstrate changes that affect viability, plasma membrane permeability ROS (Reactive Oxygen Species) in the …

Salivary Microrna As A Concussion Biomarker And The Implications For Athletic Trainers' Practices, Thomas Richard Campbell

Rehabilitation Sciences Theses & Dissertations

Many concussions are sustained globally each year; however, research suggests that a large number may go undiagnosed due to a reliance on subjective information from patients and limitations of current objective measures. Recently, efforts have been focused on identifying clinical biomarkers of concussions, including salivary microRNA (miRNA), to improve healthcare professionals’ concussion management practices. Even if salivary miRNA were shown to be a valid and reliable measure for managing concussions, healthcare professionals, such as athletic trainers (ATs), must be familiar with the tool and have positive attitudes toward the implementation into clinical practice. Therefore, the purpose of this dissertation was …

Cardiac Ablation And Stimulation With Nanosecond Pulsed Electric Fields (Nspefs), Federica Serra

Biomedical Engineering Theses & Dissertations

Surgical ablation of cardiac tissue is the definitive treatment for the most common arrhythmia, atrial fibrillation. In current medical practice, myocardial tissue is often ablated by the application of radiofrequency (RF) currents which are delivered through an electrode to create lesions that block the electrical pathways that sustain reentrant arrhythmias and restore the normal heart rhythm. An alternative to RF is cryoablation, during which the endocardial or epicardial tissue is brought in contact with a cryoprobe. Both RF ablation and cryoablation have significant limitations. In RF ablation, tissue is heated in order to ablate it, but it is an open …

Landscape Genetics Of The Gulf Coast Tick, Amblyomma Maculatum, Sara Simmons Benham

Biological Sciences Theses & Dissertations

Connectivity among populations helps to maintain genetic diversity, population stability, and resilience. The Gulf Coast tick, Amblyomma maculatum, is a vector of the pathogen Rickettsia parkeri. Persistence of tick populations with high rates of R. parkeri infection poses health risks to humans and animals. Mitochondrial haplotypes were characterized by sequencing a fragment of the mitochondrial 16S rRNA gene. A comparative study of A. maculatum and Amblyomma americanum was conducted to identify similar and unique patterns between the species within the same region. Next, I compared A. maculatum sites across three different regions of the United States. This work …

Wearable Sensor Gait Analysis For Fall Detection Using Deep Learning Methods, Haben Girmay Yhdego

Electrical & Computer Engineering Theses & Dissertations

World Health Organization (WHO) data show that around 684,000 people die from falls yearly, making it the second-highest mortality rate after traffic accidents [1]. Early detection of falls, followed by pneumatic protection, is one of the most effective means of ensuring the safety of the elderly. In light of the recent widespread adoption of wearable sensors, it has become increasingly critical that fall detection models are developed that can effectively process large and sequential sensor signal data. Several researchers have recently developed fall detection algorithms based on wearable sensor data. However, real-time fall detection remains challenging because of the wide …

E-Cadherin Force Transmission And Stiffness Sensing, Mazen Mezher

Mechanical & Aerospace Engineering Theses & Dissertations

E-cadherin is the chief mediator of cell-cell adhesion between epithelial cells and is a known mechanosensor. Force transmission and stiffness sensing are two crucial aspects of E-cadherin mechanobiology.

E-cadherin has an extracellular adhesive region, a transmembrane region and an intracellular region that binds to adhesion-associated proteins. Here, we assessed how different factors affect the level of force transmission (i) from inside the cell such as adhesion-associated proteins, (ii) on the cell membrane, such as growth factor receptors and (iii) outside the cell, such as different binding partners in adhesion. To study the level of force transmission inside the cell, we …

Coyote Occupancy And Movement In Hanover County, Virginia, Richard S. Groover

Virginia Journal of Science

Although the coyote (Canis latrans) is native to North America, we have limited understanding of its presence in Virginia. Coyote range expansion is linked to anthropogenic factors, including habitat fragmentation and the extirpation of apex predators. Information on coyote adaptations to Virginia habitats is scarce, and eastern coyotes may have unique adaptations for colonizing an area. Anecdotal evidence suggests that coyotes are abundant in Hanover County (north of Richmond, VA), but this has not been confirmed. This study was conducted over an 18-month period with multiple survey sites throughout Hanover County, each equipped with game camera stations and …