Life Sciences Commons^™

The Role Of Queuine Deficient Trna In Translational Control, Kathryn Lenore Hart

Theses and Dissertations in Biomedical Sciences

The purpose of this research was to study the effect of the level of tRNA modification with respect to queuine on translational efficiency. Transfer RNA from neoplastic cells is generally deficient with regard to certain hypermodified nucleosides found in normal cells. The hypothesis that was examined is whether or not the altered efficiency of translation can be correlated to the presence of queuine hypomodified tRNA. The loss of queuine in the anticodon may change the interaction of the tRNA's anticodon with its codon. Therefore, the translational complex may stall looking for a rare tRNA, or the stability of the complex …

Rx For Success At Back Bay National Wildlife Refuge: Take Two Committed Partners-Add Water, Janet Taylor, Julia Herrick, Ben Mathias

V. Poster Abstracts

As part of a major effort to improve habitats for waterfowl and other wetland-dependent wildlife, Ducks Unlimited, Inc. is contributing $187,500 in matching funds to help the U.S. Fish and Wildlife Service rehabilitate wetland impoundments at Back Bay NWR.

The three-year project will increase Refuge management capabilities on existing wetland areas, create 300 acres of new marsh habitat and increase water management flexibility throughout the impoundment system. Components of the project include:

• Raising and re-sloping 8 miles of existing dikes
• Installing 13 new water control structures
• Constructing 6,000 feet of new dikes
• Creating two storage pools totalling 53 acres and …

U.S. Fish And Wildlife Service Back Bay Initiative: Goals And Objectives, David A. Stilwell

V. Poster Abstracts

Back Bay has historically been noted for its abundant wildlife and fisheries. A testimony to this fact has been the establishment of two U.S. Fish and Wildlife Service National Wildlife Refuges (Back Bay and Mackey Island National Wildlife Refuges), three Virginia Department of Game and Inland Fisheries Waterfowl Management Areas (Pocahontas, Barbours's Hill, and Trojan Waterfowl Management Areas) and a State park (False Cape State Park). Back Bay has been a major stopover point for waterfowl in the North Atlantic flyway, it had prodigious submerged aquatic vegetation (SAV) beds and an outstanding fisheries. Since the l 920's these resources have …

Environmental Education: A Chance For The Future, Ben Mathias

V. Poster Abstracts

The U.S. Fish and Wildlife Service is committed to providing environmental education opportunities for the Nation's student body. Local, regional and national educators are invited to investigate and utilize the resources of Back Bay National Wildlife Refuge with their colleagues and students. Teacher workshops at the Refuge provide orientation to Refuge lands, outdoor classroom sites, trails, interpretive facilities and equipment, and potential field activities. Refuge staff members are available to assist with: • Preliminary planning • Group scheduling • Library research • Workshop registration • Trip logistics and • On-site group orientation. Most classroom sites, associated trails/boardwalks, and Visitor Contact …

Assessment Of Organic And Metal Contaminants In Lower Back Bay And Upper Currituck Sound, Kate Benkert

V. Poster Abstracts

Studies were conducted at Mackay Island National Wildlife Refuge in 1988 and 1989 to provide baseline data for an assessment of organic chemicals and metal contaminants in the aquatic ecosystem. Longnose gar, gizzard shad, snapping turtles, common rangia clams and sediments were collected from sites in lower Back Bay and upper Currituck Sound for contaminant analyses.

Composite sediment samples were analyzed for the presence of metals, organochlorine pesticides and polycyclic aromatic hydrocarbons (PAHs). Organochlorine pesticides were not detected in the sediments. Various PAH compounds were detected in the sediments, although at trace concentrations which just exceeded the analytical detection limit …

Refuge Land Acquisition: Helping Preserve Back Bay's Wildlife Heritage, Julia Herrick, Ben Mathias, Janet Taylor

V. Poster Abstracts

The once-renowned waterfowl populations and bass fishery of Back Bay, Virginia have declined dramatically in recent years. Lands surrounding Back Bay are increasingly threatened by on-going and potential land development. These lands serve as an important filter for pollutant and sediment-laden runoff from adjacent areas. The boundary of the Back Bay National Wildlife Refuge was expanded in 1989 to include an additional 6,340 acres of brackish marsh, forested swamp, and "critical edge" upland habitat, important to a variety of wildlife species and for its natural filtering effect. Within the U.S. Fish and Wildlife Service's policy of working with willing sellers, …

Cell Surface-Binding Sites For Progesterone Mediate Calcium Uptake In Human Sperm, Peter F. Blackmore, Joseph Neulan, Frank Lattanzio, Stephen J. Beebe

Bioelectrics Publications

Recent studies (e.g. Blackmore, P. F., Beebe, S. J., Danforth, D. R., and Alexander, N.) (1990) J. Biol. Chem. 265, 1376-1380) have shown that in human sperm, progesterone produces a rapid increase in intracellular free calcium ([Ca2+]i) and an induction of the acrosome reaction (e.g. Osman, R. A., Andria, M. L., Jones, A. D., and Meizel, S. (1989) Biochem. Biophys. Res. Commun. 160, 828-833). In this study, the location of progesterone receptors on the cell surface of human sperm was identified using progesterone immobilized on bovine serum albumin (BSA) (progesterone 3-(O-carboxymethyl)oxime:BSA) as well as progesterone and its 3-O-carboxymethyloxime derivative. Using …

Activator-Inhibitor Control Of Tissue Growth, John A. Adam

Mathematics & Statistics Faculty Publications

This note develops a simple model for the competition between activator and inhibitor control mechanisms in one-dimensional tissue growth. The pedagogic usefulness of such a model is that it is easily accessible to undergraduate applied mathematicians and is suggestive of behavior known to occur in more realistic biological systems (e.g., some types of cancer). The limitations of the model are obvious and can provide a basis for discussion of the applicability of complementary levels of description in mathematical modeling.

The Effects Of Glutathione On Human Keratinocyte, Manisha N. Trivedi

Theses and Dissertations in Biomedical Sciences

Cultured human keratinocytes were grown in vitro to assess the effects of glutathione, a thiol, on human keratinocyte proliferation. Single keratinocytes were harvested from newborn (greater than 5 days old) foreskin and were grown in culture flasks such that experiments could be performed in approximately 2 to 3 weeks. In order to evaluate the effects of glutathione (GSH) on keratinocyte growth, experiments were performed to determine the growth curve, plating efficiency, and incorporation of radiolabelled thymidine. Growth studies, performed with different concentrations of glutathione, revealed that 0.065 μM was the most effective concentration when compared to control cell cultures, and …

Seasonal Phytoplankton Assemblages Associated With The Chesapeake Bay Plume, Harold G. Marshall

Biological Sciences Faculty Publications

Waters associated with the Chesapeake Bay plume were highly variable, unstable, and subject to the influence of flow patterns into and out of the Bay. Seasonal phytoplankton assemblages were identified for this region, with the phytoplankton development similar to the seasonal spring-fall growth maxima over the shelf, yet influenced by the multi-pulsed patterns often noted in the lower Bay. Major dominants included species common to both the shelf and lower Chesapeake Bay.

History And Analysis Of Food Guides In The United States, Barbara B. Carlson

Health Services Research Dissertations

This work elucidates the development of nutrient-based dietary standards in the United States from the original energy and protein-based standards proposed by Atwater in 1894 to the micronutrient-based Recommended Dietary Allowances revised by the National Research Council in 1989. This qualitative historical research chronicles the development and subsequent revisions of nutrient-based food guides and food guidance models issued in the United States between 1916 and 1991. A literature search of historical food guides, research, and review papers from the fields of nutrition science and education, dietetics, and health science provided primary sources of information for the history. A literature search …

Feeding By Reticulitermes Spp., Deborah A. Waller

Biological Sciences Faculty Publications

No abstract provided.

Sediment Denitrification Potential In The Elizabeth River, Virginia, Surena Fazeli-Matin, Andrew S. Gordon, Harold G. Marshall

Biological Sciences Faculty Publications

Sediment denitrification potential from two sites in the Elizabeth River estuary was studied over a nine-month period using the acetylene blockage method. Rates of microbial processes in this environment are of interest because of the high concentration of toxics present in some parts of the system. Highest rates were found in the highly polluted Southern Branch of the Elizabeth River with nitrate amended sediment ranging from 2-262 nmol N₂0/h per 20ml of sediment and exhibiting maximal rates during spring and fall. Rates in the Main Stem of the Elizabeth River were lower, with less than 1-85 nmol N …

Observations Of The Phytoplankton Standing Crop At The Shelf Margin Of The Mid Atlantic Bight, Bruce B. Wagoner, Harold G. Marshall

Biological Sciences Faculty Publications

A comparison of the total percentage cell abundance and cell biovolume relationships of major phytoplankton categories was made between two station sets across the shelf margin. Diatom values for abundance and biovolume were greater at oceanic stations compared to the outer shelf stations, with dinoflagellates having the reverse pattern. The composite contributions to biovolume and abundance in the standing crop from other phytoplankton categories were greater over the outer shelf than beyond the shelf margin. The major source of biovolume (biomass) from the outer shelf and these oceanic stations came from the diatoms and dinoflagellates, with an average mean of …

Development And Evaluation Of Tracer Particles For Use In Microzooplankton Herbivory Studies, Mary Putt

OES Faculty Publications

Two methods of preparing algae for use as tracer particles in single species measurements of microzooplankton herbivory were evaluated. Algae were either heat-killed and labelled with 5-(4,6-dichlorotriazin-2-yl amino fluorescein) (DTAF) (Rublee & Gallegos 1989; Mar, Ecol. Prog. Ser. 51: 221-227) or stained with hydroethidine (HYD). Both DTAF and HYD-stained algae were readily visible within the digestive vacuoles of most microzooplankton collected in estuarine and coastal waters of Massachusetts (USA) and preserved with Lugol's iodine. However, DTAF was ineffective at staining several chromophytic algae and the heat-kill process reduced cell volume by ≥ 50% in several of the algae which were …

Activation In Vitro Of Transfer Rna-Guanine Ribosyltransferase By Protein Kinase C, Panayota Eriotou

Chemistry & Biochemistry Theses & Dissertations

The purpose of this study was to isolate and purify the enzymes, transfer RNA-guanine ribosyltrasferase and protein kinase C, and to determine whether the phosphorylating enzyme activates the insertion enzyme in vitro. Transfer RNA-guanine ribosyltransferase lost activity within several days after isolation and total, complete reactivation was accomplished in the presence of protein kinase C. This demonstrated that ribosyltransferase's instability is related to the degree of its phosphorylation. It is proposed that modification of tRNA is controlled by protein kinase C. Deactivation of the insertion enzyme leads to hypomodified tRNA which in turn is associated with neoplasia. Potential use …

The Effects Of Two Polycyclic Aromatic Hydrocarbons Benzo[A]Pyrene And Naphthalene On Reproductive Success Embryological Development Larval Physiology And Mortality Of The Mud Crab Rhithropanopeus Harisii (Gould), Gregory V. White

OES Theses and Dissertations

Polycyclic aromatic hydrocarbons (PAHs) such as naphthalene (Nap) and benzo[a]pyrene (B[a]P) are common in the water column and sediments of estuarine and coastal areas. The mud crab Rhithropanopeus harrisii was exposed to varying concentrations of Nap or B[a]P to determine the effects on reproductive success, embryological development, larval physiology and mortality. Nap and B[a]P decreased hatching success and prolonged embryological development. Both pollutants produced high abnormality frequencies for egg exposed larvae. Molt frequency and respiratory rates of larvae from non-exposed eggs were more chronically affected by these pollutants than were egg exposed larvae. The EC₅₀ concentrations were determined for …

Assessment Of Lipid Extraction From Human Bone On Subsequent Demineralization, Jaeeun Lee

Biological Sciences Theses & Dissertations

The purpose of this study was to assess the effectiveness of various solvents m removal of lipids from human bone, and to determine the effects of lipid extraction on subsequent acid solubilization of the mineral components of bone. Specifically, the following processing steps were studied; 1) ground human bone was de fatted in various ethanol / water mixtures, and / or chloroform / methanol ( 2: 1, v:v ), 2) ground human bone which had lipids extracted ( in absolute ethanol ) and bone which had not had lipids extracted were demineralized using vanous concentrations of hydrochloric acid to assess …

Root Biomass And Production In Four Communities Within The Great Dismal Swamp, Susan West Powell

Biological Sciences Theses & Dissertations

A biomass difference approach was used to measure biomass and production over eleven months in four different communities within the Great Dismal Swamp. On all sites, fine roots were the moat dynamic root component. Both biomass and production were highest on the mixed hardwood site, the least flooded site, and second highest on the cedar site, the site with the longest duration of saturation. The maple-gum and cypress sites had lower amounts of biomass and rates of production. Environmental parameters that influenced production included frequency and duration of flooding, density of the stand, and soil type.

A second method, an …

Laminin Potentiates Differentiation Of Pcc4uva Embryonal Carcinoma Into Neurons, T. M. Sweeney, Roy C. Ogle, C. D. Little

School of Medical Diagnostics & Translational Sciences Publications

The embryonal carcinoma PCC4uva differentiates into neurons in response to treatment with retinoic acid and dbcAMP. We used this in vitro model system to study the effects of laminin on early neural differentiation. Laminin substrata markedly potentiate neural differentiation of retinoic acid and dbcAMP-treated cultures. Only laminin induced more rapid neural cell body clustering, neurite growth and neurite fasciculation as compared to type IV collagen, type I collagen, and fibronectin substrata. Exogenous laminin substrata promoted greater cell attachment, cellular spreading and growth to confluence than type IV collagen, type I collagen, fibronectin and glass substrata. Laminin-induced effects were inhibited by …

Effects Of Applied Soil Repellent Finish On Pesticide Residues In Work Clothing, Sandra L. Prior

Community & Environmental Health Theses & Dissertations

This study explores the feasibility of using an applied soil repellent finish, Scotchgard, on 100% cotton denim fabric in order to enhance the removal of atrazine and alachlor residue during the laundering process. The experimental findings provided no statistically significant evidence that Scotchgard fabric protector reduced pesticide residuals on the 100% cotton denim fabric either before or after laundering. A significant finding secondary to the study involved the possible interaction of Scotchgard with the pesticide alachlor, leaving greater amounts of residual on the fabric after laundering than those found with no fabric treatment. This suggests that agricultural workers be cautioned …

Evidence Of A Neurogenic Component During Ige-Mediated Inflammation In Murine Skin, Victoria Jean Cavanaugh

Biological Sciences Theses & Dissertations

The direct stimulation of sensory nerves or the intradermal injection of neuropeptides triggers a cutaneous inflammatory response that involves the degranulation of mast cells. These neurogenic inflammatory reactions are physiologically similar to IgE-mediated hypersensitivity responses, which also depend on mast cells. Although non-antigenic stimuli distinguish neurogenic from IgE-mediated inflammation, the similarity of their effector stages suggested that peripheral nerves may also participate in lgE-mediated responses. To examine this hypothesis, IgE responses were elicited in denervated skin. A murine model was developed where the footpad was denervated by surgically removing a 2 mm segment of the sciatic nerve, proximal to the …

Microwell Based Solid Phase Competitive Enzyme Immunoassay For Estradiol And Estriol, Jeng-Ting Yang

Chemistry & Biochemistry Theses & Dissertations

A sensitive and simple microwell based competition enzyme immunoassay for the quantitative determination of estradiol (E₂) and estriol (E₃) was developed. Nicrowells coated with antibody were incubated with antigen followed by adding horseradish peroxidase (HRPO) conjugate. The assay, which can be performed within two hours at room temperature, involved simultaneous incubation of E₂ - or E₃-HRPO conjugate and serum sample in polystyrene microwells coated with anti- E₂ or anti-E₃ gamma globulin fraction.

Gamma globulin was isolated from whole anti-serum by DEAE-cellulose chromatography. A carbodiimide coupling method was utilized to prepare the …

Phytoplankton In Lake Drummond And Two Drainage Ditches In The Great Dismal Swamp, Virginia, Christine Gregory Phillips

Biological Sciences Theses & Dissertations

A twelve month study was conducted on the phytoplankton of Lake Drummond and Washington and Jericho Ditches from December 1988 to November 1989. Surface water samples were collected for nutrient and chlorophyll analysis from June 1989 to November 1989. The dominant phytoplankton component was the pico-nanoplankton (< 3 micron) category. A total of 57 species were identified from Lake Drummond, 51 species from Washington Ditch, and 31 species from the Jericho Ditch. Three major phytoplankton groups were identified at these sites: bacillariophyceans, cyanophyceans and cryptophyceans. The dominant species was the diatom Asterionella formosa. Nutrient and chlorophyll concentrations increased from summer to fall. Based on nutrient and chlorophyll g concentrations and species diversity indices, Lake Drummond is classified as eutrophic.

An Autecological Study Of Pyxidanthera Barbulata In The Blackwater Ecologic Preserve Virginia, John Marc Lowenthal

Biological Sciences Theses & Dissertations

Pyxidanthera barbulata (pyxie moss), a rare and endangered subshrub, has been found in decreasing numbers in it's "pine barren" habitat. An autecological study encompassing vegetation association analysis and environmental measurements was performed in the Blackwater Ecologic Preserve, its only known location in Virginia. The preserve is being subjected to controlled burning and the effect of burning on the species was evaluated. A multivariate analysis of variance was performed to identify species associations, and simple analysis of variance was done to find trends in the environmental data. Four taxa were positively associated with P. barbulata, Gaultheria procumbens, Vaccinium tenellum, Cladonia spp., …

Seasonal And Diel Patterns Of Abundance And Productivity Of Phototrophic Picoplankton In The Lower Chesapeake Bay, Lewis Francis Affronti Jr.

Biological Sciences Theses & Dissertations

This study was performed to evaluate phototrophic pico-plankton (0.2 to 2.0 μm) dynamics within the lower Chesapeake Bay. A 15 month study of phototrophic picoplankton abundance and productivity was made from June 1988 to October 1989. Annual picoplankton abundance using epifluorescence microscopy ranged from 7.26 x 10⁶ cells/1 in the winter to 9.28 x 10⁸ cells/1 during late summer.

In situ incubations of natural picoplankton populations over the 15 month study were used to test the applicability of the frequency of dividing cells technique in estimating phototrophic picoplankton growth rates. The regression equation o fμ = 2.37 x …

Development Of A Solid Phase Enzyme Immunoassay For Total Serum Testosterone, Li Li

Chemistry & Biochemistry Theses & Dissertations

An enzyme-labeled immunoassay of sufficient range and sensitivity was developed for serum testosterone determination. In this method, the testosterone in the serum sample and testosterone-HRPO conjugate compete with each other for the limited antibody binding sites on the anti-testosterone gamma globulin coated microwell. The assay can be performed in only 2.5 hours.

The gamma globulin fraction was isolated from the anti-testosterone whole serum by DEAE-cellulose column chromatography. Testosterone-peroxidase conjugate was prepared by a carbodiimide coupling method. The interference which arises from the binding of testosterone to steroid hormone binding protein was eliminated by the addition of 17 beta-estradiol. All assay …

The Determination Of Clomiphene Citrate And Tamoxifen In Human Blood Plasma And Semen, Janice Irene Kiss

Chemistry & Biochemistry Theses & Dissertations

Triphenylethylene antiestrogen compounds are used to stimulate ovulation in anovulary women and spermatogenesis in oligozoospermatic males. Clomiphene Citrate (CC) is given therapeuticaly as a mixture of the trans (E) and cis (Z) isomers. Each of these isomers has its own characteristic agonist-antagonist profiles. Only the trans form of Tamoxifen (T) is administered therapeutically.

HPLC methodologies were developed for the determination of CC and Tin human blood and seminal plasma. The methodologies involved extraction of the triphenylethylene compounds using hexane, followed by evaporation of the hexane under N₂. The residue was then taken up in a volume of HPLC …

Small Mammals In The Great Dismal Swamp Of Virginia And North Carolina, Robert K. Rose, Roger K. Everton, Jean F. Stankavich

Biological Sciences Faculty Publications

Small" mammals were surveyed in a range of habitats in the Great Dismal Swamp of Virginia and North Carolina. The survey is based on three chronologically overlapping studies, each lasting 15-18 months and for which the results have been reported separately. A different trapping method was used in each of the three studies: nest boxes, Fitch live traps, or pitfall traps. Only two species of mammals, both arboreal, were taken in nest boxes, compared with 10 and 9 species in Fitch live traps and pitfall traps, respectively. The Fitch live traps had a much higher catch rate per 1,000 trap-nights …

Reproduction In The Hispid Cotton Rat, Sigmodon-Hispidus Say And Ord (Rodentia: Muridae), In Southeastern Virginia, Robert K. Rose, Michael H. Mitchell

Biological Sciences Faculty Publications

The hispid cotton rat, Sigmodon hispidus Say and Ord, a species of the southwestern United States that has been moving northward and eastward in this century, was first observed in Virginia in 1940. In this study of the cotton rat in southeastern Virginia, most males were reproductively competent from February through November, embryos were recorded from March through October, and litter sizes were comparable to those from other locations except Kansas. Also unlike the cotton rat in Kansas, animals grew at substantial rates during the winter in Virginia. The hispid cotton rat seems to have adjusted its breeding season in …