Life Sciences Commons^™

Twenty-Seven Protein Polymorphisms By Two-Dimensional Electrophoresis Of Serum, Erythrocytes, And Fibroblasts In Two Pedigrees, David Goldman, Lynn R. Goldin, P. Rathnagiri, Stephen J. O'Brien, Janice A. Egeland, Carl R. Merril

Biology Faculty Articles

Twenty-seven independent polymorphic loci were detected by two-dimensional electrophoresis (2DE) of serum, erythrocytes, and fibroblasts in two large families and analyzed for linkage to classical genetic markers. We detected seven serum, four erythrocyte, and 17 fibroblast protein loci that exhibited charge variation in these two families and in a sample of unrelated individuals. The genetic basis of protein variants was confirmed by quantitative gene-dosage dependence and by conformance to Mendelian transmission in the two families, except for four rare variants for which transmission analysis was not possible. Linkage analysis demonstrated that each of the variants represent products of independent loci, …

The Effect Of Dispersed Oil On The Calcification Rate Of The Reef-Building Coral Diploria Strigosa, Richard E. Dodge, A. H. Knap, Sheila C. Wyers, H. R. Frith, T. D. Sleeter, S. R. Smith

Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures

Hermatypic corals represent environmentally and economically important components of the reef ecosystem. Oil spills and clean-up operations in reef areas are potential sources of pollution impact. This paper presents an evaluation of the calcification rate of specimens of the reef-building coral Diploria strigosa in response to 24 hour treatments of chemically dispersed oil at concentrations of 20 ppm. The concentrations and durations were chosen to represent a scenario of a short-term oil spill treated with dispersant passing over a coral reef.

Calcification rates were determined by the buoyant weight technique at several day intervals for up to 29 days following …

A New Marine Genus Of The Maera Group (Crustacea: Amphipoda) From Belize, James Darwin Thomas, J. L. Barnard

Marine & Environmental Sciences Faculty Articles

A new genus and species, Dumosus atari of the Maera group from coral rubble at Belize is described. It is related to Gammarella and is probably cryptic. The eyes are reduced. Dumosus differs from Gammarella in the loss of medial setation on the maxillae, reduction of article 3 on the mandibular palp, loss of article 2 on the outer ramus ofuropod 3, and in the loss of major spination on the plates of maxilla I and the maxilliped; in contrast to Gammarella, Dumosus retains the plesiomorphic uropod 3 typical of the Maera-group.

Perioculodes Cerasinus, N. Sp., The First Record Of The Genus From The Caribbean Sea (Amphipoda: Oedicerotidae), James Darwin Thomas, J. L. Barnard

Marine & Environmental Sciences Faculty Articles

Perioculodes cerasinus, a probable cryptic fossorial amphipod with embedded white orbicular ommatidia in bright ruby eyes is described from Tobago, Belize, Florida Keys, and Biscayne Bay, Florida. The eyes are separated either into two lunes or combined side to side into one large irregular brow. This is the first western Atlantic record of a generic group heretofore confined to the warm eastern Atlantic and Indian Oceans. Close affinity appears to be with the type-species of the genus, P. longimanus, from the eastern Atlantic Ocean.

Two New Species Of Two New Gammaridan Genera (Crustacea: Amphipoda) From The Florida Keys, James Darwin Thomas, J. L. Barnard

Marine & Environmental Sciences Faculty Articles

Two new species of the new genera Anamaera (A. hixom) and Spathiopus (S looensis), both belonging to the section Gammarida of Amphipoda, are described from the Florida Keys. Anamaera is a new genus close to Maera, Ceradocus, and Ceradomaera, but has an unique combination of minor character expressions. Males of Spathiopus have an unusual paddle-shaped antenna 2 but otherwise Spathiopus appears to be an apomorph of Elasmopus.

Molecular Genetic Characterization Of The Rd-114 Gene Family Of Endogenous Feline Retroviral Sequences, Roger H. Reeves, Stephen J. O'Brien

Biology Faculty Articles

RD-114 is a replication-competent, xenotropic retrovirus which is homologous to a family of moderately repetitive DNA sequences present at ca. 20 copies in the normal cellular genome of domestic cats. To examine the extent and character of genomic divergence of the RD-114 gene family as well as to assess their positional association within the cat genome, we have prepared a series of molecular clones of endogenous RD-114 DNA segments from a genomic library of cat cellular DNA. Their restriction endonuclease maps were compared with each other as well as to that of the prototype-inducible RD-114 which was molecularly cloned from …

Winds Over Water: A Bibliography, Janet M. Witte

Marine & Environmental Sciences Faculty Reports

This bibliography was compiled as an outgrowth of the Satellite Surface Stress (S3) Working Group, which was sponsored by NASA/Goddard Space Flight Center and NASA/Jet Propulsion Laboratory (JPL) from the fall of 1982 to the spring of 1984 (Contracts NAS5-26714 and 956773). I wish to thank the Working Group Chairman, Dr. James J. O'Brien, for providing me with the impetus to compile this work. I also wish to thank the other members of the Working Group for adding their moral support (as well as many of the references).

Discovery Of A Majid Host For The Commensal Amphipod Stenothoe Symbiotica Shoemaker, 1956, James Darwin Thomas, Kalani D. Cairns

Marine & Environmental Sciences Faculty Articles

No abstract provided.

Dietary Maintenance Of Bioluminescence In A Deep-Sea Mysid, Tamara M. Frank, Edith A. Widder, Michael I. Latz, James F. Case

Marine & Environmental Sciences Faculty Articles

No abstract provided.

Skeletal Extension, Density And Calcification Of The Reef Coral, Montastrea Annularis: St. Croix, U.S. Virgin Islands, Richard E. Dodge, Garrett W. Brass

Marine & Environmental Sciences Faculty Articles

Parameters of the annual and subannual skeletal growth of 61 Montastrea annularis corals, collected at a variety of shallow depth sites on reefs of St. Croix, U.S. Virgin Islands, are determined by X-radiography and scanning densitometry for each year in the 10-year period, 1970–1979. Extension (linear growth) of the coral skeleton is correlated negatively with bulk density (mass per unit volume) and positively with calcification (mass addition). Density and mass are slightly positively correlated. No one parameter, however, is a perfect predictor of another. At least two parameters, from which the third can be calculated, are required for complete description …

Two New Species Of The Siphonoecetes Complex From The Arabian Gulf And Borneo (Crustacea: Amphipoda), J. L. Barnard, James Darwin Thomas

Marine & Environmental Sciences Faculty Articles

A new species, Siphonoecetes arabicus, is described from the Arabian Gulf, and a new species B. wongi is described in the new genus Borneoecetes from Borneo. The former species does not fit recently described subgenera, thus indicating a necessary reassessment of those taxa.

Acanthohaustorius Pansus, A New Species Of Sand-Burrowing Amphipod From Looe Key Reef, Florida Keys, With Redescription And Distribution Data Of Acanthohaustorius Bousfieldi Frame, 1980 (Amphipoda: Haustoriidae), James Darwin Thomas, J. L. Barnard

Marine & Environmental Sciences Faculty Articles

A new species of sand-burrowing amphipod, Acanthohaustorius pansus, is described from carbonate sands of the Florida Keys Reef Tract. This is the first time the predominantly cold-water genus Acanthohaustorius has been reported south of Virginia coastal waters. Acanthohaustorius bousfieldi Frame, 1980, originally described from Long Island Sound, is refigured and reported from shell-hash sediments off the central Atlantic coast of Florida. A revised key to the species of Acanthohaustorius is presented.

Precise And Accurate Determination By Infrared Photometry Of Co2 Dynamics In Marine Ecosystems, Kenneth M. Johnson, Curtis M. Burney, John Mcn. Sieburth

Marine & Environmental Sciences Faculty Articles

Preliminary studies with an ampule analyzing unit and infrared (IR) detector showed that procedures for standardization and determination of total carbon dioxide (ΣCO₂), while often precise, lacked the accuracy required to estimate the net productivity and respiration of aquatic ecosystems during studies in which sampling over diel cycles was used. Scaling down sample and standard volumes to the µl range and the use of a commercial sodium carbonate standard without dilution before and after replicate sample injections gave accurate results as shown by comparison with indirect (pH-alkalinity) ΣCO₂, determinations with a standard error of ±3 µmoles …

Environmental Correlates Of Hermatypic Coral (Montastrea Annularis) Growth On The East Flower Gardens Bank, Northwest Gulf Of Mexico, Richard E. Dodge, Judith C. Lang

Marine & Environmental Sciences Faculty Articles

Time series of annual linear growth increments from 12 Montastrea annularis (E. and S.) collected at the East Flower Gardens Bank reef in the northwestern Gulf of Mexico have a common pattern. This is best expressed in an index master chronology (average by year of the annual percentage deviations from the mean of each coral.)

Comparisons with time series of environmental data indicate that coral extension rates vary positively with seasonal (February through May - 4 months) surface water temperature and negatively with annual discharge of the Atchafalaya River. We propose that secular variations of water temperature and other parameters …

Curidia Debrogania, A New Genus And Species Of Amphipod (Crustacea: Ochlesidae) From The Barrier Reefs Of Belize, Central America, James Darwin Thomas

Marine & Environmental Sciences Faculty Articles

The family Ochlesidae is amended to include the new genus Curidia, which differs from all other members by possessing maxiIIipedal palps. Curidia debrogania is described and compared to other genera and species within the family Ochlesidae.

Curidia debrogania is plesiomorphic by possession of maxillipedal palps, suggesting this member of the small, cryptic family might have originated in the tropical Western Atlantic. Distribution records and ecological notes are included.

On The Distribution And Characteristics Of Isozyme Expression In Mycoplasma, Acholeplasma, And Ureaplasma Species, Stephen J. O'Brien, Janice M. Simonson, S. Razin, Michael F. Barile

Biology Faculty Articles

A summary of a survey of three genera of mycoplasmatales (Mycoplasma, Acholeplasma, and Ureaplasma) for isozyme expression is presented. Isozyme analysis of mycoplasmas has been employed in at least three distinct areas: (1) as genetic markers for identification, individualization, and taxonomic classification; (2) as markers for cell culture contamination; and (3) as a qualitative measure of the operative metabolic pathways in the diverse species. We have found five ubiquitous enzymes: purine nucleoside phosphorylase, adenylate kinase, inorganic pyrophosphatase, dipeptidase, and esterase. Three enzymes, glucose-6-phosphate dehydrogenase, phosphogluconate dehydrogenase, and superoxide dismutase, were restricted to Acholeplasma species and were not …

Dispermic Origin And Clinical Outcome Of Three Complete Hydatidiform Moles With 46,Xy Karyotype, U. Surti, A. E. Szulman, S. O'Brien

Biology Faculty Articles

Three new cases of complete, classic hydatidiform mole with a 46,XY karyotype are described. They originated by dispermy as demonstrated by chromosome and enzyme analyses. Levels of human chorionic gonadotropin decreased to normal spontaneously within a short time, indicating a benign course in these three cases. Of a total of 18 cases reported to date, postoperative clinical information was available for 10 patients. Two of these 10 patients had a malignant course with lung metastases. It is of theoretical and clinical importance to establish the magnitude of the risk of malignancy for hydatidiform mole with a 46,XY karyotype. © 1982.

Quantitative Reef Assessment Studies In Bermuda: A Comparison Of Methods And Preliminary Results, Richard E. Dodge, Alan Logan, Arnfried Antonius

Marine & Environmental Sciences Faculty Articles

To compare in quantitative terms both ecological characteristics of the stony coral fauna at various reef sites in Bermuda and available assessment methodologies, we present a study of four separate methods at each of three reef sites. Three plotless (Intersected-length, Quarter point, Point) and one quadrat (Belt-quadrat) methods were employed. Each technique gave similar results but had inherent advantages and disadvantages which involve trade-offs in quantity and type of information generated and time required for use.

Pooling of method results revealed that total coral coverage was highest at North Rock (26%), intermediate at Three Hill Shoals (17%), and lowest at …

Evidence For The Horizontal Acquisition Of Murine Akr Virogenes By Recent Horizontal Infection Of The Germ Line, Stephen J. O'Brien, Janet L. Moore, Malcolm A. Martin, James E. Womack

Biology Faculty Articles

Several recent reports have established the biological and molecular genetic similarity between the endogenous AKV virus of strain AKR, and an N-ecotropic endogenous virus found in the genome of feral Japanese mice, Mus musculus molossinus. The similarities are so striking as to suggest a common origin of these viruses, which are present in some, but not all, inbred mouse strains. The virogenes of AKR mice may have been acquired by either: (a) common descent of AKR (and other AKV⁺ strains) from a common ancestor of AKR and molossinus animals, or (b) horizontal germ line infection of the AKR …

Notes On Recent Changes In Tanaidacean Terminology, Charles G. Messing

Marine & Environmental Sciences Faculty Articles

no abstract available

Mouthpart Morphology And Feeding Strategies Of The Commensal Amphipod, Anamixis Hanseni Stebbing, James Darwin Thomas, George W. Taylor

Marine & Environmental Sciences Faculty Articles

The lack of the information on the biology of the commensal amphipod family Anamixidae prompted investigations into mouthpart ultrastructure, feeding biology, and host specificity of Anamixis hanseni Stebbing, 1897. Earlier investigators presented conflicting reports on the structural composition of the buccal region and feeding modes in anamixids. Scanning electron microscope analysis of A. hanseni showed a complete set of mouthparts to be present, although much modified and reduced, except for the well-developed maxilliped palps. Feeding is not parasitic (piercing and sucking host tissue) as previously believed but is accomplished while A. hanseni rests inside its host. Minute food particles are …

Analysis Of Multiple Isoenzyme Expression Among Twenty-Two Species Of Mycoplasma And Acholeplasma, Stephen J. O'Brien, Janice M. Simonson, Marion W. Grabowski, Michael F. Barile

Biology Faculty Articles

Crude extracts of triple-cloned, purified cultures of 22 species of Mycoplasma and Acholeplasma were examined for expression of 21 isozyme systems routinely used to type mammalian cells. Nine previously described enzymes (purine nucleoside phosphorylase, adenylate kinase, dipeptidase, esterase, glyceraldehyde-3-phosphate dehydrogenase, glucose phosphate isomerase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and superoxide dismutase) and three enzymes not previously reported in mycoplasma (triose phosphate isomerase, inorganic pyrophosphatase, and acid phosphatase) were detected in some or all of the species examined. These findings provide new information on the enzymatic expressions of these organisms. Three of the isozyme systems (superoxide dismutase, glucose-6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase) …

Seasat Altimetry Adjustment Model Including Tidal And Other Sea Surface Effects, Georges Blaha

Marine & Environmental Sciences Faculty Reports

This study is a part of the on-going effort aimed at improved determinations of the earth's gravity field through the adjustment of satellite altimeter data in the short-arc mode. Until recently the key role in such adjustments has been played by GEOS-3 altimeter data. However, SEASAT altimetry is envisioned as providing a more accurate means for addressing this task.

In view of the improved quality of altimeter data and of the corresponding more stringent requirements for the data reduction, several improvements in the existing altimetry model have been designed and are described herein. For example, the criteria have been established …

Growth Characteristics Of Reef-Building Corals Within And External To A Naval Ordinance Range: Vieques, Puerto Rico, Richard E. Dodge

Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures

The skeletal growth of reef·building corals is known to be sensitive to the environment. In particular, high levels of sedimentation and turbidity lead to decreased growth rate, suppressed growth variation, and, ultimately, coral death because of reduced illumination necessary to zooxanthellae and/or increased energy expenditure by the coral animal to remove impacted sediments.

To assess the effect of Naval Ordnance Range usage at Vieques, Puerto Rico, specimens of Montastrea annulariswere collected from reefs adjacent to and removed from the range area. Growth was measured from annual increments revealed by X-radiography of medial slabs of the coral skeletons. Mean growth …

1979 Hurricane Damage To Coral Reefs Of Vieques, Puerto Rico, Richard E. Dodge, William F. Raymond

Marine & Environmental Sciences Faculty Reports

No abstract provided.

Pagurapseudes (Crustacea: Tanaidacea) In Southeastern Florida: Functional Morphology, Post-Marsupial Development, Ecology And Shell Use, Charles Messing

Oceanography Faculty Theses and Dissertations

Members of the genus Pagurapseudes occupy vacant microgastropod shells. Functional and behavioral convergence with paguridean decapods (hermit crabs) is treated using a combination of gross morphological, histological and scanning electron microscopic analyses, as well as observations of living animals. Post-embryological development is described and compared with that of other tanaidaceans. The proposed life history differs from that of other tanaidaceans in several respects and is the first described for any member of the suborder Monokonophora based on investigations using experimentally reared specimens. Variations in a population over the course of 1.5 years are discussed as are apparent habitat preferences. Preferences …

X-Rays Provide Researchers With Views Of Coral Growth Patterns, Richard E. Dodge

Marine & Environmental Sciences Faculty Articles

No abstract provided.

Atmospheric Forcing Of The Bight Of Abaco, Peter M. Smith

HCNSO Student Theses and Dissertations

No abstract provided.

A New Species Of Cerapus Say, 1817 (Crustacea: Amphipoda) From The Northern Gulf Of Mexico, With Notes On Its Ecology, James Darwin Thomas, Richard W. Heard

Marine & Environmental Sciences Faculty Articles

During research in coastal marshes of the Mississippi Delta region in 1975, a large, undescribed amphipod belonging to the genus Cerapus was discovered in tidal channels. This new species, C. benthophilus, is characterized by its large size and 7-12 segmented antennal flagella. Habitat preference is muddy silt bottoms upon which it constructs conspicuous mats or "tufts" of interwoven tubes in shallow coastal bays and marshes from Louisiana to the panhandle areas of northern Florida.

A Redescription Of The Wood-Rasping Amphipod Trophichelura Gomezi Ortiz, 1976 (Cheluridae) From The Florida Keys, With Notes On Its Distribution And Ecology, James Darwin Thomas

Marine & Environmental Sciences Faculty Articles

The wood-rasping amphipod, Tropichelura gomezi Ortiz, 1976, is redescribed from the Florida Keys to include diagnostic characters omitted in the original description. Morphological characters and distributional patterns of T. gomezi are compared with those of the other member of the genus, Tropichelura insulae (Caiman, 1910). Laboratory studies show that T. gomezi defends its burrow entrance from other members of its species, but tolerates the presence of limnoriid isopods with which it cooccurs. A lectotype for Tropichelura insulae is designated .