Life Sciences Commons^™

Assessing The Effect Of Nutritional Interventions On Body Composition Changes During Pregnancy In Moderately Malnourished Malawian Women, Megan K. Kitagawa

Master's Theses

Background: Malnutrition can affect the body composition changes that occur in healthy pregnancy, resulting in adverse effects to both the mother and infant. Supplementary foods with high quality protein may result in more favorable changes in lean mass and fat mass in pregnant malnourished women.

Objective: To retrospectively assess changes in body composition in moderately malnourished pregnant Malawian women in response to one of three nutritional interventions.

Methods: Height, weight, MUAC, and TSF were measured in pregnant women with MUAC >20.6 cm and19) were compared using ANOVA. If differences were detected, differences between groups were tested using the Tukey HSD …

Forest Densification Over 85 Years In A Sierra Nevada Mixed-Conifer Forest Decreases Conifer Regeneration And Limits Survival, Marissa A. Vossmer

Master's Theses

Forest densification in response to a century of fire suppression in Sierra Nevada mixed conifer forests has decreased conifer regeneration and survival. Increases in overstory biomass and decreases in canopy heterogeneity, along with decreases in shrub cover in the understory, has created unfavorable establishment site conditions for conifer species. Establishment site conditions are key in promoting germination and establishment of conifers seeds and in determining the survival of these seedlings into the overstory. These changes in establishment site suitability resulting from the removal of disturbance from these forests has decreased conifer regeneration and survival into other age classes. In Sierra …

Proteomic Analysis Of The Crustacean Molting Gland (Y-Organ) Over The Course Of The Molt Cycle, Talia B. Head

Master's Theses

Molting in crustaceans is a highly complex physiological process involving negative regulation by two paired endocrine glands, the X-organ/sinus gland complex (XO/SG) and the Y-organ (YO). The XO/SG complex is responsible for making molt-inhibiting hormone (MIH) which negatively regulates synthesis of the molting hormones, ecdysteroids, by the YO. Analysis of gene expression in the XOs and YOs has led to the development of a proposed molecular signaling pathway which regulates ecdysteroidogenesis and subsequent molting in crustaceans. In this study, changes in protein abundance in the YO were characterized over the course of a molt cycle (intermolt, early premolt, mid premolt, …

Characterization Of Reagent Pencils For Deposition Of Reagents Onto Paper-Based Microfluidic Devices, Cheyenne H. Liu, Isabelle C. Noxon, Leah E. Cuellar, Amanda L. Thraen, Chad Immoos, Andres W. Martinez, Philip J. Costanzo

Chemistry and Biochemistry

Reagent pencils allow for solvent-free deposition of reagents onto paper-based microfluidic devices. The pencils are portable, easy to use, extend the shelf-life of reagents, and offer a platform for customizing diagnostic devices at the point of care. In this work, reagent pencils were characterized by measuring the wear resistance of pencil cores made from polyethylene glycols (PEGs) with different molecular weights and incorporating various concentrations of three different reagents using a standard pin abrasion test, as well as by measuring the efficiency of reagent delivery from the pencils to the test zones of paper-based microfluidic devices using absorption spectroscopy and …

Designing An Authentic Behavioral Research Experience In The Classroom Using Siamese Fighting Fish, Betta Splendens, Gianne Souza, Ronald M. Coleman

STAR Program Research Presentations

Recently there have been major shifts in biology education towards emphasizing the teaching of the scientific process to more closely reflect actual scientific practices. Authentic research experiences require the development of many higher order cognitive skills. Creating an authentic research experience that appropriately scaffolds the development of these complex skills while engaging individual student motivation remains challenging in classroom settings. Some common challenges include limited time available for working with large numbers of students, cramped working areas, and limited funding and materials resources. Using the Siamese Fighting Fish, Betta splendens, we have created an economically viable authentic animal behavioral …

Ecological And Organic Life Safety Concerns Of Hfo-1234yf, Alberni V. Ruiz

STAR Program Research Presentations

While at NOAA my research was directed by the inquiry of whether or not HFO-1234yf, a new compound being introduced into coolant system and replacing greenhouse gases HFC, is harmful to organic life. Using the computer program Matlab I was able to develop figures that represented the concentration of HFO-1234yf (HFO) in 12 different tower locations throughout the country. Comparing and contrasting concentrations of this HFO compound at each site led me to the question the possible harmful side effects this compound could bring to organic life due to the fact that concentration levels are increasing, with one factor being …

Strategies For Increasing The Release Of Pigments In Red Wine, Briana Heywood

Master's Theses

The perception of wine’s quality is directly influenced by its color. Anthocyanin molecules are responsible for imparting color to red wines. They are extracted from grape skins during alcoholic fermentation. This work compares the effects of three parameters: berry integrity, enzyme addition, and fermentation temperature, on phenolic compound extraction (total phenol, tannin and anthocyanin) during the production of Paso Robles’ Cabernet Sauvignon wine. Analyses on phenolic compounds were completed during alcoholic fermentation and barrel aging over the course of eighteen months. Berry integrity compared the degree of berry crushing (whole destemmed berries versus fully crushed berries). Results showed that phenolic …

Heavy Metals In Seaweeds From A Polluted Estuary In Coastal Maine, Ian D. Medeiros, Arthur C. Mathieson, Nishanta Rajakaruna

Biological Sciences

Seaweeds (macroalgae) accumulate heavy metals from seawater and can therefore serve as biological monitors of marine pollution. In this paper, we present data on the tissue chemistry of seaweeds from the area of Callahan Mine, an intertidal copper and zinc mine in Brooksville, ME, USA, abandoned in the 1970s and now designated as a Superfund site. The mine is located on the Goose Pond estuary, which drains into the ocean via a tidal waterfall at Goose Falls. Seaweed tissue samples from this estuary had the same relative concentrations of metals as had been reported previously in water samples from this …

A Comparative Analysis Of The Nutrient Composition And Digestibility Of California Perennial And Annual Grasses At Four Stages Of Growth, Elaina Cromer

Master's Theses

Beef products represent the fourth largest agricultural commodity in the state of California, valuing more than $3 billion from 2013 to 2015 (CDFA, 2016) and procure 90% of the income for the range livestock industry (FRAP, 2003). Forages found on California’s coastal, desert, foothill, and mountain ranges are the basis of the state’s beef cattle industry. Understanding their nutritional quality of these forages is important for their effective use (George et al., 2001a; Waterman et al., 2014). The objectives of this research were to investigate the nutritional characteristics, and in situ digestbilities in Angus beef cattle, of common California annual …

Maternal Distraction During Breast- And Bottle Feeding Among Wic And Non-Wic Mothers, Alison K. Ventura, Simone Teitelbaum

Kinesiology and Public Health

Objective: To explore the prevalence and correlates of maternal distraction during infant feeding within a sample of mothers enrolled or not in the Special Supplemental Nutrition Program for Woman, Infants, and Children (WIC).

Design: Mothers kept diaries of their infants’ feeding patterns.

Participants: Mothers (n = 75) with infants aged ≤6 months.

Main Outcome Measures: Within the diaries, mothers recorded what else, if anything, they did during the feeding. Mothers also completed questionnaires on demographics, feeding styles, and infant temperament and eating behaviors.

Analysis: Mothers’ responses were coded into thematic categories. Feedings were classified as distracted when the mothers reported …

Incorporating Instruction Into The Huasna Valley 4-H Beef Group, Carly P. Chandless

Agricultural Education and Communication

The purpose of this project was to design a manual to be used by 4-H leaders and members. The author wants to develop an interesting and intriguing manual that will demonstrate how to raise a market steer. This manual will contain step by step instructions on how fit a steer for show and how to halter break. These illustrations will help guide a 4-H member through the process of handling their steer. The Huasna Valley 4-H Steer Manual will provide beneficial information, to the leader and member, from the moment the project is purchased to sale. More importantly, the manual …

Working Bodies: A Photo Essay On Tulare County Citrus Workers, Isamar Hernandez-Ramos

Agricultural Education and Communication

Many a times, when people think about their food, they think about origins. Where did it come from? Was it hygienic? Were the animals treated humanely? They seldom include the workers in their thought process, though the workers of the Ag industry provide what is arguably a vital part of the Ag industry. They do back-breaking labor in often lethal conditions for little pay (NFWM). The families of these workers often suffer economically, physically, and emotionally. In Tulare County, one of the top Agricultural counties in the country, the agricultural worker is often below the poverty line (Taylor, 2015). They …

Consumer Agricultural Education: Starting At The High School Level Health Class, Cecilia Teresa Acosta

Agricultural Education and Communication

The purpose of this project is to analyze and understand the communication gap between the farmer and consumer in order to create a dialogue about misconceptions in the media. Often rumors in the media worry or mislead the consumer in his or her buying choices. In order for consumers to understand what they are buying, and critically analyze media information, it is necessary to teach agricultural education at the high school level health class. This project will showcase how to start this education in order to bridge the communication gap starting with five crucial and common food-packaging labels. These labels …

Pink And Dude Chefs: Efficacy Of An Online Train-The-Trainer Mechanism And Student Program Outcomes, Jacqueline Chen

Master's Theses

Background: The rate of overweight and obesity among adolescents aged 12-19 years has quadrupled since 1980. Reducing obesity is a key public health priority, as obesity is associated with individual and population-level health and economic consequences. Afterschool-based obesity prevention programs that aim to develop nutrition and culinary self-efficacy have shown promise. However, the level of aptitude among program instructors is directly correlated with student success, suggesting the importance of effective train-the-trainer (TTT) mechanisms for implementing and scaling up these strategies.

Pink and Dude Chefs (PDC) is an afterschool nutrition education and culinary skills program for middle-school adolescents aged between 11-14 …

Peromyscus Boylii And Peromysus Californicus Differ In Physical Characteristic Measurements, Puja M. Kasondra

Biological Sciences

I provide a comparison of physical characteristics between Peromyscus boylii and Peromyscus californicus. All mice compared were captured in the dominant regions of the Los Padres National Forest during three different years – 2005, 2011, and 2014. The dominant regions of the Los Padres National Forest include Riparian, Woodlands, and Chaparral. All characteristics, ear (from notch), head and body, hind foot, tail, and weights, were compared between males of each species and then between females of each species. All data were analyzed in JMP 12. Out of all the characteristics, tail and weight had the greatest difference between the …

Linking Large Scale Ocean-Atmospheric Patterns With Recruitment In Kellet’S Whelk (Kelletia Kelletii), Katherine N. Rodriguez

Biological Sciences

Global warming influences the biogeography of many marine and terrestrial species. Understanding species range shifts is ecologically and socioeconomically important when guiding management decisions for ecosystems exposed to a rapidly changing climate. In this natural experiment, I follow Danielle Zacherl’s methods (Zacherl et al. 2003) to study the effects of large-scale ocean-atmospheric patterns on recruitment of a marine snail, Kellet’s whelk (Kelletia kelletii); these organisms recently expanded their range North past the geographic barrier of Point Conception to Monterey, CA, USA. I use shell length data collected at 32 subtidal rocky reef kelp forest sites in 2015 (before …

Hanford Ffa Ornamental Horticulture Unit Master Plan, Savanah Diane Rhine, Russell Alan Zimmerman

Agricultural Education and Communication

This project is intended to develop a more functional plan for the Ornamental Horticulture (OH) Unit at the Hanford Agriculture Program. The ag program combines all three high schools in Hanford resulting in over 1,000 students; however, a majority of these students are not in the OH Career Pathway due to a lack of a constant, fully functional unit. As a comprehensive agriculture program, it is crucial that it incorporates all aspects of agriculture, not just animal science and agricultural mechanics.

This project will provide the Hanford agriculture program with a plan to be implemented so that they may have …

Rabbit Showmanship Guidebook, Mitchell B. Liwanag

Agricultural Education and Communication

Rabbit Showmanship Guidebook

A Senior Project

Presented to

The Faculty of the Agricultural Education and Communication Department

California Polytechnic State University, San Luis Obispo

In Partial Fulfillment

of the Requirements for the Degree

Bachelor of Science

By

© Mitchell Liwanag

June 2017

Introduction: Every year 4-H, FFA, and Independent exhibitors compete in rabbit showmanship at hundreds of county and state fairs as well as rabbit shows. It is the author’s opinion with the proper guidance, showmanship can be simple and easy to learn. However, without the help of someone knowledgeable, showmanship is complicated. By creating a showmanship guide new leaders …

Cost Of Feed Per Pound Of Gain In Cal Poly's Dairy Calves, Kayle Anna Phelan

Agricultural Education and Communication

California is ranked one of the top five states in the United States for dairy production. It produces approximately 40 billion pounds of milk per year for US production. California’s dairy industry is not only the largest milk producer in the nation, but it is also the most financially prominent in the agricultural industry. This industry lead is purely because of its careful management practices. The way calves are raised depend on the resources used, the natural environment present, and the operation of the dairy farm. Essentially, there are numerous ways to care for a cow and calf. However, the …

Developing Visual Aids For Ffa Promotion In The Classroom, Audrey Y. Lent

Agricultural Education and Communication

The purpose of this project is to create visual materials for a classroom that the teacher can use as a supplement for information taught about FFA activities and programs shared with students.

The FFA advisor whose chapter would be the beneficiary of the project was consulted to discuss the best subject material for the posters. One poster was created for Supervised Agricultural Experience (SAE) projects, one for Career and Leadership Development Events (CDEs and LDEs), one for the California FFA State Conference, and one for career opportunities in the agricultural industry. These four posters were developed using an online program …

The Recovery Of Protein From Egg Yolk Protein Extraction Granule Byproduct, Irene Jennifer Kaufman

Master's Theses

In addition to proving an excellent source of nutrients, eggs are used in the food, cosmetic, and biotechnology industries for their rheological and bioactive properties. Much of the potential for the added value is in individual components of the egg, rather than the whole egg. At low speed centrifugation, yolk separates into two distinct fractions—granules and plasma. It is becoming increasingly popular in the industry to remove the plasma fraction of the egg yolk to use for its livetins, particularly immunoglobulin Y, leaving behind a granule by-product (“yellow cake”). Previous research has shown potential added-value from the granule fraction, especially …

Marine Gastrobot Final Design Report, Wesley B. Williams, Eric Kane Dreischerf, Tommy Nicholas Yath

Mechanical Engineering

The Marine Gastrobot sponsored by Dr. Christopher Kitts of the Cal Poly Center for Applications in Biotechnology was a research and development effort intended to explore the use of microbial fuel cell technology as a power source for underwater robots. Our team Ocean Locomotion succeeded in developing a first iteration of an underwater robotic platform suitable for microbial fuel cell integration. The primary feature of the design is its sinusoidal fin propulsion intended for benthic exploration with limited risk of entanglement. During the course of development, Ocean Locomotion explored the use of low power actuation methods and determined their limited …

Marine Biomass Analyzer, Kai Jay Staal, Brian Paris, Tyler Cottle

Mechanical Engineering

This report documents the design, fabrication, and testing for a marine biomass analyzer. The goal of the project and constructed system was to determine the validity and efficacy of a process that could flatten benthic macrofauna to a consistent thickness such that a biomass for the collected sample could be accurately determined. The system that was built and tested consists of a drive train that turns a mill subassembly where the organisms are flattened, supply and collection spools that hold the white fabric and clear film used to capture the organisms, a collection zone where the sample is injected, and …

Assessing Soft Skills At The Cal Poly State Agriculture Ambassador Conference, Mary Luann Allen

Agricultural Education and Communication

In today’s society, there is a disconnect with the agriculture industry, agriculture schools, and the public. Students need to acquire soft skills to aid in furthering the agriculture industry through their careers. Overall, hiring managers found soft skills such as communication, leadership, ownership, and teamwork were missing in this new crop of workers (Payscale/Future Workplace, 2016).

College Agriculture Ambassadors advocate for agriculture, higher education, and their respective schools all across the United States. The Cal Poly Agriculture Ambassadors club provides leadership, communication, and personal development to its members through meetings, activities and responsibilities.

The Cal Poly Agriculture Ambassador club hosted …

Effects Of Food Consumption On Cell Proliferation In The Brain Of Python Regius, Stacy Star Habroun

Master's Theses

Neurogenesis is an important and vastly under-explored area in reptiles. While the ability to generate new brain cells in the adult mammalian brain is limited, reptiles are able to regenerate large populations of neuronal cells. Pythons exhibit a characteristic specific dynamic action (SDA) response after food intake with an increase in metabolic rate that facilitates processing the meal. Associated with this change in SDA, pythons (Python spp.) also exhibit impressive plasticity in their digestive and cardiovascular physiology due to the sheer magnitude of the increase in organ growth that occurs after a meal to speed digestion, absorption, and assimilation of …

The Population Genetics Of Morro Bay Eelgrass (Zostera Marina), Julia Gardner Harencar

Master's Theses

Seagrass populations are in decline worldwide. Zostera marina (eelgrass), one of California’s native seagrasses, is no exception to this trend. In the last 8 years, Morro Bay, California has lost 95% of its eelgrass. Eelgrass is an ecosystem engineer, providing important ecosystem services such as sediment stabilization, nutrient cycling, and nursery habitats for fish. The failure of recent restoration efforts necessitates a better understanding of the causes of eelgrass decline in this estuary. Previous research on eelgrass in California has demonstrated a link between population genetic diversity and eelgrass bed health, ecosystem functioning, and resilience to disturbance and extreme climatic …

Paper Microzone Plates As Analytical Tools For Studying Enzyme Stability: A Case Study On The Stabilization Of Horseradish Peroxidase Using Trehalose And Su-8 Epoxy Novolac Resin, Kirsten A. Ganaja, Cory Chaplan, Jingyi Zhang, Nathaniel W. Martinez, Andres W. Martinez

Chemistry and Biochemistry

Paper microzone plates in combination with a noncontact liquid handling robot were demonstrated as tools for studying the stability of enzymes stored on paper. The effect of trehalose and SU-8 epoxy novolac resin (SU-8) on the stability of horseradish peroxidase (HRP) was studied in both a short-term experiment, where the activity of various concentrations of HRP dried on paper were measured after 1 h, and a long-term experiment, where the activity of a single concentration of HRP dried and stored on paper was monitored for 61 days. SU-8 was found to stabilize HRP up to 35 times more than trehalose …

Associations Between Breastfeeding And Maternal Responsiveness: A Systematic Review Of The Literature, Alison K. Ventura

Kinesiology and Public Health

Recent recommendations and prevention programs have focused on the promotion of responsive feeding during infancy, but more research is needed to understand best practices for fostering responsive feeding during early life. The objective of this systematic review was to synthesize the accumulating bodies of evidence aimed at understanding associations between mothers' feeding experiences and responsive feeding in an attempt to clarify the nature of associations between feeding mode and responsive feeding. A literature search was conducted between January and October 2016; articles were collected from PsychINFO, Medline, and CINAHL, as well as from references in published research and reviews. Article …

Expierience, Seong Bo Chang, Albert Chen, Emily Nguyen

Computer Engineering

The name of this project is called “exPIERience” and the technological field of focus is virtual reality. We will be working on creating a virtual reality experience using the Oculus Rift for the Center of Coastal Marine Sciences (CCMS). The purpose of this system is to enable people to experience diving at the Cal Poly Pier even during nonoptimal diving conditions which occur frequently. CCMS hopes to attract further interest and possible momentum in future outreach such as a “Live Dive” program, where live videos of divers under the pier can be streamed. A 360° video from the diver’s perspective …

Ultramafic Geoecology Of South And Southeast Asia, M.L. Galey, A. Van Der Ent, M.C.M. Iqbal, N. Rajakaruna

Biological Sciences

Globally, ultramafic outcrops are renowned for hosting foras with high levels of endemism, including plants with specialised adaptations such as nickel or manganese hyperaccumulation. Soils derived from ultramafic regoliths are generally nutrient-deficient, have major cation imbalances, and have concomitant high concentrations of potentially phytotoxic trace elements, especially nickel. The South and Southeast Asian region has the largest surface occurrences of ultramafic regoliths in the world, but the geoecology of these outcrops is still poorly studied despite severe conservation threats. Due to the paucity of systematic plant collections in many areas and the lack of georeferenced herbarium records and databased information, …