Life Sciences Commons^™

Migration Of Elk (Cervus Canadensis) And Barriers To Movement, Levi Watkins

Theses and Dissertations

Movement patterns of animals are varied, complex, and can be influenced by environmental and anthropogenic factors. One form of animal movement, migration, is influenced by environmental factors that alter the timing, duration, intensity, and likelihood of migration. Additionally, features of the landscape, both natural and anthropogenic, can alter how animals move through their seasonal and home ranges. Movement patterns can be impeded or prevented by features such as railroads, rivers, and roads. Here we explore characteristics of elk (Cervus canadensis) migration by evaluating the strategies of migration exhibited and the factors that influence migration of elk in central and eastern …

Overcoming Barriers To Native Species Restoration Using Gibberellic Acid And Fungicide Seed Coatings, Amber Jo Johnson

Theses and Dissertations

Many barriers can limit restoration success. In the first chapter of this thesis, the barrier of strong seed dormancy is addressed. While dormancy benefits the species' long-term survival, it can present a challenge within a restoration scenario where rapid establishment is required. Soaking seeds in gibberellic acid (GA3) can overcome dormancy. An easier and potentially more effective method to apply this hormone is to coat seeds with a GA3-impregnated polymer, which provides a slow release of the hormone. Seed dormancy can also be mitigated by creating a favorable microsite with increased soil moisture. We compared the emergence and establishment of …

Development Of A Valid And Reliable Survey To Assess Personal, Behavioral, And Environmental Factors Surrounding Food Insecurity In A College Population, Jennette Kilgrow

Theses and Dissertations

Objective: The aims of our present study were to develop a valid and reliable survey framed around the social cognitive theory, which would evaluate internal and external factors associated with food insecurity from the student's perspective, and to test the survey with a multi-state population. Design: The College Perspectives around Food Insecurity (CPFI) survey was developed through several revision and improvement steps using a mixed methods approach. In Phase 1 (2018-2019), data were translated into survey items and assigned to a theoretical concept (personal, behavioral, or environmental) in the social cognitive theory. In Phase 2 (2019-2020), content validity testing occurred …

Descriptions, Ecological Associations And Predictive Species Distribution Models Of New Species Of Psilochalcis Kieffer (Hymenoptera; Chalcididae) Occurring In Utah's Eastern Great Basin, Mark J. Petersen

Theses and Dissertations

The genus Psilochalcis, of the Family Chalcididae, was originally described in 1905 by Kieffer. Mainly considered an Old-World taxon, the first North American Psilochalcis were not identified until 1981 by Grissell and Schauff. Little is known about the species distributions, biologies and ecological relationships of these parasitic wasps. This dissertation describes research conducted in central Utah setting arrays of Malaise traps in 4 different habitat types common to the Great Basin at three separate locations. A result of this sampling revealed a high abundance of multiple species of Psilochalcis wasps, particularly from one location and two habitat types. Chapter 1 …

Unusual Bird Reports From The Trans-Pecos District Of Chihuahua, Mexico, Including Five New Additions To The State Of Chihuahua, Israel Moreno-Contreras, Fernando Mondaca-Fernández, Daniel Enrique Pérez-Carrillo, José Manuel Jurado-Ruiz, María Cristina Pérez-Amezola, Marisa Tirado-Madrid, William H. Howe

Western North American Naturalist

We present new distributional information on 21 species of birds from the state of Chihuahua, Mexico, 5 of which represent the first documented records for this state. Several observations are of birds normally associated with the Sierra Madre Occidental province, suggesting that these species may be regular wanderers to the Trans-Pecos district of Chihuahua.


Presentamos nueva información sobre 21 especies de aves del estado de Chihuahua, México. Cinco de ellas representan el primer registro documentado para Chihuahua. Nuestras observaciones adicionales son de aves asoci­adas con la provincia de la Sierra Madre Occidental, sin embargo, estos registros sugieren que estas especies …

Prevalence And Predictors Of Intern-Related Burnout In Dietetics Preceptors, Holly Hopkins

Theses and Dissertations

Dietetics preceptors are at risk for abandoning the role of preceptor because of potential burnout. Quantifying the prevalence of burnout and identifying the predictors of burnout can help key stakeholders identify and implement strategies that prevent or mitigate burnout in dietetics preceptors. The purpose of this study was to determine the prevalence of intern-related burnout in dietetics preceptors and to identify the predictors of this burnout. This study used a cross-sectional study design. A survey instrument that included burnout and precepting measures, and items about the respondents’ personal and work characteristics was distributed to a random sample of 10,000 dietetics …

Forest Understory Plant Community Response To Prescribed Fire And Mechanical Thinning Treatments, Noelle V. Zenger

Theses and Dissertations

Much of the biodiversity in a forest comes from the understory vegetation. However, centuries of fire suppression in the western United States have made human-implemented disturbance necessary for reinstating natural disturbance cycles. However, the mechanical thinning and prescribed burning treatments used by land managers may differentially affect the response of the understory community. This study aimed to compare how prescribed fire and mechanical thinning treatments, with the size and severity of those treatments, affect understory plant communities in aspen-conifer forests over time. We sampled the understory community in burned, mechanically thinned, and untreated forest stands one, three, or five years …

Floral Visitors Of A Colorado Endemic Chasmophyte, Telesonix Jamesii (Saxifragaceae), Andrew G. Gaier, Erin Manzitto-Tripp, Julian Resasco

Western North American Naturalist

Telesonix jamesii, a rare and imperiled species of perennial saxifrage, is restricted to rocky habitats at high elevations across 21 isolated, known populations in the southern Rocky Mountains of Colorado and New Mexico. Despite its imperiled conservation status, very little is known about the natural history of T. jamesii. We studied pollination of this species during the summers of 2019–2021 at multiple locations on Pikes Peak, Colorado. We conducted a total of 899 min of pollinator surveys, identifying all floral visitors during this time period. We then examined floral visitors for the presence of T. jamesii pollen to …

A Comparison Of Kid Goat Lipase And Microbial Lipase On The Development Of Cheddar Cheese Flavor, Reece H. Larsen

Theses and Dissertations

Background: Manufacturers continue to look for methods to save time in aging cheese. Complex reactions occur during cheese ripening that produce characteristic flavors and aromas. The addition of exogenous lipase enzymes is one accelerated ripening method that has been studied. Our objective was to document and compare the flavor profiles of cheddar cheeses created with either microbial lipase or animal lipase. Materials and Methods: The experiment followed a conventional formulation to create experimental batches of cheddar cheese. Kid goat lipase and three microbial lipase treatments were compared against a control. Other animal lipases were evaluated in preliminary research but were …

Dopamine D2 Receptors As A Peripheral Biomarker For Brain Dopamine Levels, Christina Small

Theses and Dissertations

The ability to objectively index dopamine (DA) levels in the brain has the potential to revolutionize the field of neuropsychopharmacology, as having a peripheral biomarker of brain DA would enable the objective monitoring of the progression of Parkinson's disease (PD) and other DA-dependent psychiatric states. Of particular relevance to commercialization, it would provide an objective measure of treatment efficacy. We used a DA-depletion approach to determine if peripheral D2Rs are a biomarker for brain DA; mainly, the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) neurotoxin model and PD subjects, which are well-known models of DA depletion in the midbrain of rodents and humans, respectively. Dopamine …

New Perspectives On Foot Segment Forces And Joint Kinetics - Integrating Plantar Shear Stresses And Pressures With Multi-Segment Foot Modeling, Dustin A. Bruening, Spencer R. Petersen, Sarah T. Ridge

Faculty Publications

The role of the foot in gait is not well understood, in part due to challenges in capturing and utilizing segmental ground reaction forces. The purposes of this study were to 1) develop methodology to integrate plantar pressure/shear stresses with a multi-segment foot model, and 2) generate and display key normative data from this combined system. Twenty-six young healthy adults walked barefoot (1.3 m/s) across a pressure/shear sensor with markers matching a published 4-segment kinetic foot model. A novel anatomical/geometric masking method was developed that successfully separated regions aligned with model segmentation. This template-based method can be automated and customized …

Halophilic Genes That Impact Plant Growth In Saline Soils, Mckay A. Meinzer

Theses and Dissertations

Many plants are highly sensitive to salt in the soil, and their growth and yield can be greatly hindered by as little as less than 1% salt concentration in the soil. Additionally, soil salinity is a growing issue globally and affects significant areas in Utah. Halophytes are plants that are adapted to grow in saline soils and have been widely studied for their physiological and molecular characteristics, but little is known about their associated microbiomes. Bacteria were isolated from the rhizosphere and as root endophytes of Salicornia rubra, Sarcocornia utahensis, and Allenrolfea occidentalis, three native Utah halophytes. Several strains of …

Utilizing Isothermal Titration Calorimetry To Measure Β-Galactosidase Activity In Dairy Products, Tyler Ronald Jarrard

Theses and Dissertations

The dairy industry uses enzymes to make cheese, alter product flavor, and eliminate lactose. The activities of these enzymes have been measured in clear buffered solutions, but because of the limitations of spectrophotometric methods, enzyme activities have not been measured in opaque or colored dairy products where they are used. Isothermal titration calorimetry (ITC) can be used to determine reaction kinetics in opaque and colored solutions by measuring the heat rate from enzyme-catalyzed reactions as a function of time. This study used ITC to measure β-galactosidase activity in opaque solutions of milk, sweet whey, sweet whey permeate, acid whey, and …

Development And Validation Of A Spanish Nutrition Screening Tool For Hispanic American 3 To 5-Year-Olds, Denisse Arias Olivas

Theses and Dissertations

Latinos comprise 18.9% of the population in the United States and are the largest and fastest growing minority group. Obesity prevalence was 26.2% among Hispanic children compared to 16.6% among non-Hispanic White children. The obesity epidemic among Latino children has been growing rapidly over the past three decades. Multiple barriers, such as lack of culturally appropriate screening tools, language, and lack of access to nutritional assessment and expert consultation, prevents screening of Latino children and further nutritional guidance. For this reason, prevention efforts such as the use of nutritional screening tools is required for early intervention, more so with populations …

Real-Time Imaging And Measurement Of Compartmentalized Redox Shifts Using Novel Redox-Sensitive Biosensors: Implications In Developmental Toxicology, Brandon Mitchell Davies

Theses and Dissertations

Glutathione (GSH) is a small antioxidant in the body and exists in large quantities compared to other antioxidants. The GSH redox state (Eh) helps developmental processes, however, when the GSH Eh is disrupted, cells may undergo significantly poor developmental pathways, possibly leading to long-lasting damages. Similarly, NADPH and Thioredoxin redox states can have a major impact on cellular function, viability, and response to both endogenous and exogenous toxicants. Subcellular, compartmentalized redox environments during normal or perturbed situations, specifically in the cytosol, mitochondria, and nucleus, are not well understood. Here, using the P19 neurogenesis model of cellular differentiation, the kinetics of …

A Comparison Of The Vascular Response To Acute Sauna Heating In Young And Middle-Aged Adults, Olivia Kathryn Leach

Theses and Dissertations

BACKGROUND: Age-related declines in endothelial function have been well documented with larger declines observed in middle-aged. Passive heat exposure has been shown to be a promising method to improve vascular endothelial health, with sauna specifically being linked to reduced risk of cardiovascular disease. Increases in blood flow and shear rates associated with heat exposure are often considered to have a major influence on the observed improved endothelial function following heat exposure. The magnitude of these changes in response to sauna have not yet been defined. Therefore, the purpose of this study is to quantify and compare the vascular response to …

Thermal Inactivation Of Salmonella, Escherichia Coli, And Enterococcus Faecium Nrrl B-2354 In Pasta Matrices, Kristi Shannon Gowans

Theses and Dissertations

Limited data are currently available characterizing the thermal resistance of foodborne pathogens in semolina flour and intermediate pasta matrices representative of commercial conditions during mixing, extrusion, and drying. These data are essential to pasta producers seeking to be compliant with federal regulations since Salmonella spp. and Escherichia coli demonstrate survival in wheat flour and dried pasta products. This study investigated the heat resistance of Salmonella, pathogenic E. coli, and E. faecium NRRL B-2354 in raw semolina flour and partially dried pasta intermediates via thermal death time (TDT) studies. This study also assessed the appropriateness of E. faecium NRRL B-2354 as …

Developing Tools For Introducing Modifications Into The Chicken Genome, Zachary Eldon Olsen

Theses and Dissertations

The chicken is a classic model organism that has provided key insights into embryonic development. Chicken embryos can be directly manipulated and observed during development while retaining the potential to reach adulthood. Despite this benefit, the utility of the chicken in studying development has been limited by the difficulty of introducing genetic changes to the genome. The recent development of cell culture conditions for chicken primordial germ cells (cPGC) has made it feasible to produce transgenic chickens, but there is still a lack of tools for introducing genetic modifications into cPGCs. Recombinase Mediated Cassette Exchange (RMCE) is a technique that …

Comparing Multiple Sclerosis Age Of Onset In American American And European Cohorts Via Polygenic Risk Scores, Amy Arabel Hernandez

Undergraduate Honors Theses

Health disparities have been observed in autoimmune diseases such as multiple sclerosis (MS), which show that non–Hispanic black patients with MS die at an earlier age and have an increasing mortality trend compared to white MS patients.³ New genetic information shows promising results to treat genetic diseases. For example, genome-wide association studies (GWAS) are being used to calculate polygenic risk scores (PRS) which are a method used to understand disease risk. However, current PRS have been developed using GWAS that overrepresent individuals of European ancestry,⁴ showing that they do not equally apply to all individuals. We set out …

Creating Habitat Suitability Models For Restoration Of Sesbania Tomentosa And Chenopodium Oahuense On Molokai, Hawaii, Bronwyn Meldrum

Undergraduate Honors Theses

Hawaii is home to incredible diversity in both plant and animal life. Nearly 90% of Hawaii’s 1,400 plant taxa are found nowhere else in the world, and in an ecosystem threatened by invasive species and terrain alteration, this puts them at risk of extinction. As pressures on global biodiversity intensify, conservation efforts and restoration of endangered species are of the utmost importance. The Molokai Land Trust is actively working to eradicate invasive kiawe (Prosopis pallida) forests and to restore endemic species and other native vegetation on the Mokio Preserve. These vital efforts are made more difficult because the …

Identifying And Knocking Out Deep Brain Photoreceptors In The Larval Zebrafish Optic Tectum, Allison Pickens

Undergraduate Honors Theses

Deep brain photoreceptors (DBPs) are light sensing neurons present in mammals and non-mammalian vertebrates. In non-mammalian vertebrates, studies have shown DBP involvement in various time and light dependent behaviors including circadian rhythm, phototaxis, and seasonal reproduction. In adult teleosts, DBPs are present throughout the brain, including the optic tectum (OT), a multilaminated midbrain structure responsible for multisensory integration. While their approximate location in teleosts is known, little is known about DBP’s functionality. In this study, we focused on DBPs present in the larval zebrafish OT. Single-cell RNA sequencing of the larval zebrafish OT showed the presence of several opsins. We …

Effects Of The Ketogenic Diet On Spatial Memory And Long-Term Potentiation In The Ca1 Region Of The Hippocampus In Young Rodents, Jed Christensen

Undergraduate Honors Theses

The ketogenic diet (KD) originated as a treatment for epilepsy nearly a century ago, though its neurological effects are not completely understood. In recent years the diet has resurged as a weight loss aid, reinvigorating research investigating its effects on the nervous system. Notably, the KD alters the concentration of glucose and ketones in the brain, and recent research suggests that the elevated ketone concentration, in the form of beta-hydroxybutyrate, induced by the diet enhances recognition memory and mitochondrial efficiency in the hippocampus. To further explore the effects of the KD, we examined its cognitive effects on spatial memory and …

Genetic Stop Signs: Nontraditional Sequences That Terminate Rna Production, Christina Iverson, Samuel Scott

Library/Life Sciences Undergraduate Poster Competition 2023

3rd place open house

Gene expression can change everything about the identity of a bacterial cell. This process begins when the protein RNA polymerase reads a DNA strand and produces, or transcribes, a matching messenger RNA sequence. This RNA sequence will then be read by a protein-building complex which builds a protein encoded by the RNA. When RNA polymerase finishes producing the RNA sequence, the RNA polymerase will fall off the DNA strand, known as transcription termination. Currently, there are two known mechanisms for transcription termination. We believe that there may be more unknown mechanisms for termination.

Isolating Highly Specific Antibodies Against Cancer Target Hprt, Austin J. Hansen, Christopher J. Haynie, Kiara V. Whitley, Michelle Townsend, Abby Johnson, Hunter Lindsay, Laura Baird, Kim L. O'Neill

Library/Life Sciences Undergraduate Poster Competition 2023

Effective immunotherapeutic targeting of cancer cells requires identification of specific antigenic differences between cancerous and healthy cells. Hypoxanthine-guanine phosphoribosyltransferase (HPRT) is a cytoplasmic enzyme involved in the salvage pathway for DNA synthesis. Certain cancers (e.g., prostate and breast) upregulate and express HPRT on the cell membrane, making HPRT a potential cancer biomarker for cancer immunotherapies. Antibodies specific for unique cancer proteins are critical to develop targeted cancer immunotherapies. Using a yeast display library and cell sorting, we selected for high-affinity human single-chain (scFv) antibodies against HPRT that can be utilized in chimeric antigen receptor (CAR) T cell therapies and antibody-dependent …

Phage Host Interactions In Fhua Dependent Bacteriophages, Ian W. Baeten, Jessica M. Lewis, Kathryn E. Janda, David E. Kotter, Julianne H. Grose, William R. Mccleary

Library/Life Sciences Undergraduate Poster Competition 2023

Bacteriophages are the natural enemy of bacteria. They infect and kill bacterial cells similarly to how animal viruses infect and kill our (eukaryotic) cells. Being viruses, bacteriophages need to get inside of their host to be able to replicate and survive. To do this, phages use receptor binding proteins (RBPs) to bind to various molecules on the surface of bacterial cells, which allows them to attach and initiate the first step of infection. Several novel bacteriophages were isolated from sewage samples across the Wasatch Front of Utah that infect Escherichia coli strain MG1655 and are being used to gain greater …

Quantifying The Effects Of Involvement In Aesthetic Sports On The Development Of Eating Disorders In Pre-Teens, Ella Cook, Melissa Hewitt, Anna Sherrill, Alyssa Tolman, Erik J. Nelson

Library/Life Sciences Undergraduate Poster Competition 2023

Approximately 2.8 million people (ANAD, 2023) in the United States are affected by eating disorders, and more than 1 in 5 children (Holcombe, 2023) around the world suffer from disordered eating symptoms. Eating disorders have the highest mortality rate of any mental illness, and children involved in “aesthetic sports” (dance, wrestling gymnastics, swimming) are at a higher risk of developing symptoms than their non-participating peers. (Davison et al., 2002) Eating disorders are often associated solely with an older teenagers or women, but heavily impact children as well. It is impossible to address mental health concerns among America’s youth without addressing …

Flipped Versus Online Instruction: A Comparative Analysis Of Student Performance, Noah Emery, Jamie L. Jensen

Library/Life Sciences Undergraduate Poster Competition 2023

The need for improved modes of online instruction has rapidly increased within the last decade. But whether online instruction is beneficial or as effective as in-person remains inconclusive (McCutcheon et al., 2015). Evidence would suggest that there are knowledge gaps with students who enroll in online courses (Jensen et al., 2022).

We designed and tested various models of an online introductory biology course for non-biology undergraduates in order to determine which method of online instruction would most improve student performance and close these knowledge gaps. We hypothesized that including instructor scaffolding (instructor organization and support), instructor feedback, and peer collaboration …

Higher Incidence Of Parasitic Copepods In Female Host Rockfish Species Call For Improved Restoration And Commercial Management Strategies, Alyssa Walters, Mark C. Belk

Library/Life Sciences Undergraduate Poster Competition 2023

Host-parasite interactions are key components in shaping ecosystem and community structure. In parasitism, the host maintains the life of the parasite and can have its fitness influenced as a result. This can be especially problematic for commercial fish projects, as well as species management.

New Species Discovery In Eriastrum (Polemoniaceae) In The Californian Red Mountains, Kaitlyn Pankratz, Leigh Johnson

Library/Life Sciences Undergraduate Poster Competition 2023

Characterized in the flowering plant family Polemoniaceae by wooly hairs on the calyces and bracts associated with the flowers and found in dry areas of western North America (1).

The Effects Of A High Carbohydrate Vs. High Fat Pre-Fast Meal On Incretin Hormone Secretion: A Randomized Crossover Study, Parker Graves, Landon S. Deru, Bruce Bailey

Library/Life Sciences Undergraduate Poster Competition 2023

Chronic illness such as strokes, heart disease and diabetes all rank among the leading causes of death in the United States. Recently, fasting has gained popularity as a means of prevention from chronic illness through weight loss and other health benefits. Fasting produces multiple beneficial physiological responses that have been shown to aid in chronic disease prevention, one of which is observed by the release of incretin hormones such as glucosedependent insulin tropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1). These incretin hormones are released by the gut to augment the secretion of insulin to regulate postprandial glucose levels. During a …