Life Sciences Commons^™

Reducing Food Scarcity: The Benefits Of Urban Farming, S.A. Claudell, Emilio Mejia

Journal of Nonprofit Innovation

Urban farming can enhance the lives of communities and help reduce food scarcity. This paper presents a conceptual prototype of an efficient urban farming community that can be scaled for a single apartment building or an entire community across all global geoeconomics regions, including densely populated cities and rural, developing towns and communities. When deployed in coordination with smart crop choices, local farm support, and efficient transportation then the result isn’t just sustainability, but also increasing fresh produce accessibility, optimizing nutritional value, eliminating the use of ‘forever chemicals’, reducing transportation costs, and fostering global environmental benefits.

Imagine Doris, who is …

A Vision For A Food-Secure Future Through Permaculture, Jim Gale

Journal of Nonprofit Innovation

In response to the pressing challenges of food security, sustainability, and resilience, Food Forest Abundance, led by visionary CEO Jim Gale, emerges as a beacon for permaculture-based agriculture and community development. This editorial explores the organization's pivotal role in advocating for a future where individuals, families, and communities thrive through sustainable practices and localized food systems. Central to Food Forest Abundance mission is the conviction that permaculture principles can revolutionize our approach to food security, health, and wellness by uniting agricultural and community leaders.

The catalyst for this movement was the World Farmers Market Coalition report, revealing the tangible economic, …

Fungicide Data, Amber Johnson

ScholarsArchive Data

This dataset contains emergence, biomass, and fungal biomass data collected during a laboratory study examining the effects of soil fungal level and seed coating treatment on the growth of bluebunch wheatgrass (Pseudoroegneria spicata). Pathogenesis from soil and seed-borne fungus can limit success when restoring native species. Fungicides can combat fungal pathogens, but in some studies, fungicides treatments are ineffective at improving seedling emergence over untreated, control seed. These studies suggest low fungal presence due to dry conditions may be the cause of the ineffectiveness of fungicide treatments for some years and sites. This study tested if fungicide treatment …

Bridging The Gaps: Characterizing Alzheimer's Disease In Native Hawaiian And Pacific Islander Populations, Justina P. Tavana

Theses and Dissertations

This dissertation addresses critical gaps in dementia research in Native Hawaiian and Pacific Islander (NHPI) communities. Focusing on Samoan and Tongan communities, the study successfully adapted and validated the AD8 informant interview and the Clinical Dementia Rating (CDR) scale in native languages, establishing these instruments as reliable tools for detecting cognitive impairment in these populations. The research process prioritized meaningful community engagement and forged partnerships with indigenous professionals, ensuring alignment with and responsiveness to the specific needs of the community. These invaluable connections were the foundation of making this difficult work possible. Subjects assessed with these adapted instruments were enrolled …

Plant Material Selection And Seed Enhancement Technologies Improve Seed-Based Restoration In Dryland Systems, Travis G. Sowards

Theses and Dissertations

The restoration of large-scale heterogeneous landscapes is typically approached using seed-based restoration as a cost-effective means to achieve project goals and objectives. However, seeding is often met with low survival rates primarily driven by low seedling emergence and establishment. This dissertation investigates opportunities to enhance restoration focusing on 1) seed treatments, 2) plant materials, and 3) a synthesis of seeding strategies. • Fungal pathogenesis can cause high seedling mortality. Fungicide seed coatings used in the agriculture industry are novel to wildland restoration. We assess the effect a fungicide seed coating has on the recruitment of Pseudoroegneria spicata seedlings across a …

Effect Of Timing Of Energy Intake On Blood Glucose And Body Composition In Women With Overweight Or Class One Obesity, Michael Wade Smith

Theses and Dissertations

Overweight and obesity has increased in the U.S. Unfortunately, this increases the risk of metabolic dysfunction, such as diabetes. Meal timing has recently shown promise as viable options to minimize the risk of overweight or obesity and metabolic dysfunction. The primary purpose of this study, therefore, was to investigate the effect of timing of energy intake on blood glucose in females with overweight or class one obesity (BMI=27-35 kg/m2). This study utilized crossover design with two conditions counterbalanced in random order. Participants completed two 2-week 1500kcal dietary interventions: 1) a big breakfast diet or BB (50% of energy intake between …

Linking Individual Behavior With Physiology To Understand Complex Life Histories, Doreen Cabrera

Theses and Dissertations

Life history strategies encompass a suite of co-evolved traits crucial for ensuring an individual's fitness, often evolving from trade-offs between traits competing for the same resources. Adult male crayfish in the Cambaridae family undergo seasonal morphological changes linked to reproductive status, a process called cyclic dimorphism. In this dissertation, we attempt to understand how cyclic dimorphism evolved in non-native northern crayfish (Faxonius virilis). First, in Chapter 1, we review empirical studies that have assessed animal personality across developmental periods. The results show that personality traits tend to be stable within life stages but not across developmental events. In Chapter 2, …

Leucine Lock: An Amplified Rapid Antigen Detection System, Matthew David Goff

Theses and Dissertations

Rapid diagnostic tests for COVID-19, influenza, and blood analytes currently have many drawbacks. They vary greatly in accuracy, are expensive to manufacture, and can be insufficiently sensitive to small antigen loads. Leucine lock is designed to rectify these issues that exist in current rapid diagnostics by creating a rapid antigen test with minimal background activity, tunable sensitivity, and low manufacturing cost. Leucine lock consists of a novel leucine zipper containing an intramolecular hairpin, an ScFv antigen binding domain, and a fluorophore reporter in a FRET combination. By altering the number of hydrophobic and electrostatic mismatches in the intramolecular hairpin, as …

The Role Of Myocyte Enhancer Factor 2d In Beta-Cells: Implications For Glucose Homeostasis And Cell Survival, Jacqueline Elise Crabtree

Theses and Dissertations

Myocyte Enhancer Factor 2D (Mef2D) is a member of the Mef2 family. As a transcription factor, Mef2D regulates the expression of genes that impinge on cellular viability, tissue development, and fuel metabolism. Interestingly, Mef2D function appears to be tissue dependent. The function of Mef2D appears to be tissue-specific, with differences in activity in the immune system, neuronal tissue, and muscle. Here, we review the published literature describing the role of Mef2D across tissues. Little is known about the role of Mef2D in the beta-cell. To determine the function of Mef2D in the beta-cell, we built overexpression and knockdown cell lines. …

The Effect Of Percussive Massage On The Maintenance Of Skeletal Muscle Health During Limb Unloading, Mohadeseh Ahmadi

Theses and Dissertations

A period of skeletal muscle disuse leads to muscle mass and strength loss and compromised metabolic health. Several studies suggest that passive mechanical stimulation may protect against the detrimental effects of disuse in skeletal muscle. This study assessed the effect of the twice-daily application of percussive massage in mitigating the adverse effects of muscle disuse during ten days of leg unloading. Seventeen participants (7 women and 10 men; 18-35 yrs.) were randomly assigned to either control or massage groups, both undergoing leg unloading. The data revealed that both groups similarly experienced significant reductions in muscle strength (-14%±5.9; p<.0001), quadriceps volume (-4.2%±2.8; p<.0001), myofiber cross-sectional area (-8.2%±1.8; p<.0001), and maximal mitochondrial respiration (-20%±9.1; p<.0001). Fatty acid-supported respiration decreased in the control group (-30.4±18.5%; p= 0.0008) and remained unchanged in the massage group. Hydrogen peroxide (H2O2) flux normalized to oxygen consumption exhibited a significant interaction between group and time, particularly in the absence of ADP, where H2O2 flux increased only in the control group (control: 29.9±26.5%, massage: -13±22.6%; p=0.01). There was no change in the H2O2 flux after the addition of ADP. In conclusion, ten days of limb unloading resulted in diminished muscle size, strength, and mitochondrial respiration. Percussive massage was insufficient to prevent the loss of muscle mass, force production, or carbohydrate-supported respiration. However, it exhibited potential protective effects against the decline in mitochondrial fatty acid-supported respiration.

Genomic And Proteomic Analysis Of Five Vi01-Like Phages Reveals Wide Host Range And Multiple Tail Spike Proteins, Evan Harris

Theses and Dissertations

The number of illnesses attributable to antibiotic resistant bacterial infections are on the rise. According to the CDC, by 2050, complications due to antibiotic resistant infections are set to become the most prevalent cause of death worldwide, overtaking both cancer and heart disease in number. We are required to look for supplements to aid antibiotics in the fight against bacteria because it's becoming increasingly clear that antibiotics alone will fail in the face of multidrug-resistant strains. Bacteriophages are natural killers of bacteria and can thus be weaponized to treat multidrug-resistant infections. When a phage is discovered, our databases need to …

Characterizing Stress Granule Regulation By Pas Kinase, Ataxin-2 And Ptc6 And Investigating The Lifespan Of Covid-19 Virus On Currency, Colleen R. Newey

Theses and Dissertations

The protein Ataxin-2 is a known positive regulator of stress granules in humans, mice and yeast (known as yeast PBP1). Due to the role that stress granules play in diseases including Amyotrophic Lateral Sclerosis (ALS) and cancer, this thesis investigates the role of Ataxin-2 and its protein binding partners in stress granule development and its effects on various metabolic phenotypes of the cell. PAS kinase is a sensory protein kinase, conserved from yeast to man, which regulates respiration and lipid biosynthesis. Our lab discovered that PAS kinase phosphorylates and activates Ataxin-2 in yeast, and that PAS kinase overexpression enhances localization …

Soil Health In American Sports Fields, Miria C. Barnes

Theses and Dissertations

Healthy soils are essential for sustaining the world's ecosystems and maintaining human lifestyles. The adoption of biological, chemical, and physical analyses to assess soil health is a relatively new concept with a paucity of scientific work assessing how these metrics are affected by field management in urban systems Soil samples (n = 110) were collected from a diverse range of sports fields and, for comparative purposes, golf courses, farm fields, non-sport urban, undisturbed forest, and non-vegetated sandy soils. The samples were then analyzed using biological, chemical, and physical metrics to determine if there were significant differences between sport/golf venues and …

Use Of Microcalorimetry To Evaluate Hardening Reactions In Protein Bars During Accelerated Storage, Tiffany Rose Spackman

Theses and Dissertations

Protein bars have become a popular option among consumers to increase protein content in their diets. Since there is a large market for protein bars, many factors must be considered when creating a protein bar that both satisfies consumers and has a long shelf-life. Hardening and textural changes in protein bars are some of the most common modes of shelf-life failure in this product category. When the typical product creation timeline from formulation to launch can be as short as 3-6 months and with added pressure from executives to quickly launch another new product afterwards, product development scientists simply do …

Novel Fire And Herbivory Regime Impacts On Forest Regeneration And Plant Community Assembly, Devri A. Tanner

Theses and Dissertations

Human activities are increasing the occurrence of megafires that have the potential to alter the ecology of forest ecosystems. The objective of this study was to understand the impact of a 610 km2 megafire on patterns of forest regeneration and herbivory of three forest types (aspen/fir, oak/maple, and pinyon/juniper) within the burn scar. Sapling density, meristem removal, and height were measured across a transect network spanning the area of the burn scar over three years from 2019-2021. The network consisted of 17 burned/unburned transect pairs in adjacent burned/unburned forest stands (6 aspen/fir, 5 oak/maple, and 6 pinyon/juniper). Species that regenerated …

Tidygeo Preparing Analysis-Ready Datasets From Gene Expression Omnibus, Avery Mecham, Ashlie Stephenson, Badi I. Quinteros, Grace S. Brown, Stephen R. Piccolo

Faculty Publications

TidyGEO is a Web-based tool for downloading, tidying, and reformatting data series from Gene Expression Omnibus (GEO). As a freely accessible repository with data from over 6 million biological samples across more than 4000 organisms, GEO provides diverse opportunities for secondary research. Although scientists may find assay data relevant to a given research question, most analyses require sample-level annotations. In GEO, such annotations are stored alongside assay data in delimited, text-based files. However, the structure and semantics of the annotations vary widely from one series to another, and many annotations are not useful for analysis purposes. Thus, every GEO series …

Rage In Chronic Pulmonary Inflammation And Obstetric Complications, Katrina Lynn Curtis

Theses and Dissertations

The receptor for advanced glycation end-products (RAGE) is a transmembrane cell surface protein of the immunoglobulin superfamily that acts as part of both the innate and adaptive immune system. RAGE is highly expressed in lung tissue and is therefore of interest in the pulmonary immune response. Specifically, RAGE mediates several cell-signaling responses such as inflammation and apoptosis. This work sought to elucidate the role of RAGE in the setting of chronic pulmonary irritation such as that found in long-term exposure to secondhand smoke (SHS). This irritation has several shared characteristics with lung diseases such as chronic obstructive pulmonary disease (COPD) …

Mechanisms, Evolution, And Rapid Diagnosis Of Carbapenem Resistance, Taalin Shale Hoj

Theses and Dissertations

An estimated 70% of bacterial infections in the United States are resistant to first-line antibiotics. Of these, among the most difficult to treat are highly resistant to carbapenems, an antibiotic class used as a last resort. Septicemia, particularly when caused by a carbapenem-resistant organism, is among the most challenging clinical scenarios to treat, with an overall mortality rate of carbapenem-resistant septicemias 63.8%. Encouragingly, if effective antimicrobial treatment begins within 1-3 hours of septic shock onset, patient survival rates are around 80%. The first section of this work describes a real-time PCR-based assay that when coupled with existing bacteria-blood separation technology, …

Elucidating The Architecture Of The Tclijn Complex That Converts Cysteine To Thiazoles In The Biosynthesis Of Micrococcin, Diana G. Calvopina Chavez

Theses and Dissertations

Thiopeptides are a family of antimicrobial peptides that are characterized for having sulfur-containing heterocycles, and for being highly post-translationally modified. Numerous thiopeptides have been identified; almost all of which inhibit protein synthesis in gram-positive bacteria. These intrinsic antimicrobial properties make thiopeptides promising candidates for the development of new antibiotics. The antimicrobial peptide micrococcin is a thiopeptide that is synthesized by the ribosome and undergoes several post-translational modifications (PTMs). Micrococcin is formed from a precursor peptide, TclE. TclE comprises an N-terminal leader (35-AA) that is crucial for recognition of the PTM machinery, alongside a C-terminal core sequence (14-AA) that undergoes multiple …

The Impact Of Appearance-Related Communication In Families On Female Adolescent Body Image Development, Cambria Siddoway

Family Perspectives

No abstract provided.

Dyadic Coping In The Transition To Parenthood, Sierra Gray

Family Perspectives

The Transition to Parenthood (TTP) is a common transition point for couples that is often associated with declines in relationship satisfaction. There are many changes that take place during this transition that can lead to a variety of stresses such as psychological distress, sexual distress, as well as increases in conflict. Parents likely experience these stressors together, and therefore should implement dyadic coping processes. With partner support and responsiveness, parents can counter damaging psychological outcomes. As partners incorporate empathy and communicate effectively throughout the TTP, they can prevent and improve sexual outcomes. Since partner conflict is very common during this …

More Than Fun And Games: Why Play Matters, Leah Merrill

Family Perspectives

No abstract provided.

How To Make Prison Visits A Success, Ellie Anderson

Family Perspectives

No abstract provided.

Manipulating And Assaying Chromatin Architecture Around Enhancer Elements In Vivo, John Lawrence Carter

Theses and Dissertations

There are about 20,000 genes in the human genome. The lowly nematode worm, C. elegans, has about the same number of genes. How could two organisms that are so different arise from a similar number of genes? The answer is epigenetics, or the factors that help control when and where genes are expressed. There are many layers that comprise the epigenetic control of genes. One of which is the structure or architecture of chromatin. Chromatin is a complex of DNA and proteins. Histone proteins with DNA wrapped around them form the fundamental component of chromatin, the nucleosome. Chromatin exists in …

Cell Of Origin Identification Using Methylation Signatures From Seminal Cell-Free Dna And Heterogenous Cellular Mixtures, Ryan Barney

Theses and Dissertations

Infertility is an issue for approximately 12% of couples attempting to have a child. Of this group, 50% of the cases are due to male factor infertility. There are many reasons for decreased fecundity in men, but there remains 10% to 15% of infertile men that are diagnosed with the most severe form of infertility, non-obstructive azoospermia (NOA). A diagnosis of NOA implies the lack of sperm cells in the ejaculate with no physiological reason. The current diagnostic test and treatment consist of microscopic examination of seminal fluid and a biopsy to extract any viable sperm from the testis. This …

A Novel Application Of Spinning Disk Technology To Collect Plasma From Whole Blood Prior To Quantifying Plasma Rna, Naomi Rapier-Sharman, Mae-Lynn L. Hutchinson, Carlos M. Moreno, Abraham Quaye, Brian D. Poole, K. Scott Weber, William G. Pitt, Brett E. Pickett

Faculty Publications

The spinning disk technology has previously been utilized to isolate bacterial components from blood in hours instead of days. We hypothesized that this platform could be applied as an alternative approach to isolating plasma RNA from a whole blood sample. We consequently tested the efficacy of the spinning disk technology to extract plasma from whole blood upstream of RNA isolation and analysis. To do so, we collected plasma using either the spinning disk or the typical two-spin centrifuge method. We found that the spinning disk method results in significantly more hemolysis during collection than the conventional two-spin centrifuge method. However, …

Changes In Rna Expression Of Hut78 Cells Resulting From The Hiv-1 Viral Protein R R77q Mutation, Joshua S. Ramsey

Theses and Dissertations

Human immunodeficiency virus type 1 (HIV-1) is the causative virus for acquired immunodeficiency syndrome (AIDS). AIDS is characterized by chronic inflammation and reduction of CD4+ T-cells in the blood. This leads to the patient becoming immunocompromised and much more susceptible to disease in general. Different phenotypes in the progression of AIDS have been observed in patients in either progressing to AIDS faster as a Rapid Progressor (RP), or slower as a Long-Term Non-Progressor (LTNP). Researching elements that result in the LTNP phenotype is of interest as it has the potential to offer alternative treatments and therapies to those suffering from …

Ecosystem Structure And Function Across Western Dryland Ecosystems: A Cross-Site Comparison Of Semiarid Ecosystem Types In Colorado And Wyoming, Christopher W. Beltz, William K. Lauenroth, Ingrid C. Burke

Western North American Naturalist

Drylands extend over large portions of the globe, including a significant share of the western United States, yet they remain understudied. We examined ecosystem structure and function at 3 semiarid dryland sites in Colorado and Wyoming, each of which was dominated by a different ecosystem type and plant community—shortgrass steppe, mixed-grass prairie, and sagebrush steppe. This initial study was conducted to fill gaps in knowledge about how sagebrush steppe compares to dryland grasslands in the western United States. Soil respiration was higher at the sagebrush steppe than at the 2 grassland sites (P = 0.001). Aboveground biomass, microbial abundance, …

Field-Based Selenium Partitioning Coefficients, Trophic Transfer Factors, And Otolith Time-Series Analyses For A Walleye Community From An Ecotonal Plains Reservoir, Colorado. Iii., Scott J. Herrmann, Del Wayne R. Nimmo, Lynn M. Herrmann-Hoesing, Igor V. Melnykov, Norman M. Halden

Western North American Naturalist

Little is known about partitioning coefficients, tissue bioaccumulation, trophic transfer, and otolith timeseries analyses of selenium (Se) in a fishery dominated by walleye (Sander vitreus) that consume multiple prey species and occur in a Se-rich reservoir. Concentrations of dissolved total Se in Pueblo Reservoir water consistently exceeded the current EPA criterion benchmark for lentic aquatic ecosystems. The major objectives of this study were to analyze Se in diet-borne consumables of a walleye fishery and perform time-series trend tests of 21 walleye sagittae. In contrast to previous reports, we proposed investigating Se partitioning from water to subsequent trophic levels …

Spatial Ecology Of The Texas Alligator Lizard (Gerrhonotus Infernalis) In Blanco County, Texas, Corey M. Fielder, Wade A. Ryberg, Danielle K. Walkup, Jared M. Holmes, Toby J. Hibbitts

Western North American Naturalist

Texas Alligator Lizards (Gerrhonotus infernalis) range from Central Texas into adjacent northeastern Mexico, and published ecological studies on their terrestrial and arboreal movement patterns are lacking. We used radiotelemetry to assess movements, annual home range, and arboreal activity of G. infernalis at Bamberger Ranch Preserve, Blanco County, Texas, to provide baseline ecological data for populations occurring in the northernmost extent of the species’ range. Movement patterns were influenced by temporal (e.g., seasonal) and behavioral cues (e.g., breeding period), but generally no differences were observed between sexes. Annual home range varied, but males averaged larger 95% MCP (minimum convex …