Life Sciences Commons^™

Characterization Of A Stenotrophomonas Maltophilia Bacteriophage, Edward Eshoo

College of Science and Health Theses and Dissertations

Stenotrophomonas maltophilia is a ubiquitous Gram-negative, multidrug resistant, opportunistic bacterial pathogen that causes various infections in humans. Recently, the use of bacteriophages as therapeutic agents, has gained interest as an alternative to traditional antibiotics. This thesis describes the isolation, purification, and characterization of S. maltophilia bacteriophage Bfi2 and discusses its activity against related, and often co-isolated, bacterial pathogens. Amplification of the phage resulted in clear, well-defined plaques and a titer of 1.73 ± 0.38 x 1011 PFU/ml. Bfi2 demonstrated the ability to lyse 55% of the S. maltophilia strains tested, suggesting that it has a moderate host range. However, the …

Isolation Of Stenotrophomonas Maltophilia Virus Bfi1: A Biofilm Inhibiting Phage, Thomas Edward Moran

College of Science and Health Theses and Dissertations

The bacterium Stenotrophomonas maltophilia is an emerging infectious pathogen of global concern. Due to its drug-resistant nature, there are limited treatment options available. A potential option for combating S. maltophilia infections is phage therapy, the medicinal use of viruses to treat bacterial infections. Stenotrophomonas phage Bfi1 was isolated from a soil sample using S. maltophilia clinical strain S18202. Transmission electron microscopy provided evidence that this phage is a member of the Siphoviridae family. Host range analysis showed that the phage successfully infected and lysed 30% of the S. maltophilia strains tested. Genomic analysis revealed that the phage contains approximately 32.2-56.5 …

Investigating The Viability Of Two Stenotrophomonas Maltophilia Isolates After Air-Drying, Samantha Lane, Joanna Brooke

DePaul Discoveries

Abstract

Stenotrophomonas maltophilia is a water-borne infectious bacterium that is found in both clinical (hospitals) and non-clinical environments. This human pathogen is commonly recovered from respiratory tract infections. A recent study at a hospital in Taiwan suggested that dry patient charts can serve as a vehicle of transmission of this bacterium⁷. As S. maltophilia is not commonly isolated from dry surfaces, this current study tested the hypothesis that this pathogen can remain viable for some time on a dry surface. This study was designed to determine how long S. maltophilia could remain viable after air-drying by observing …