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Articles 1 - 4 of 4
Full-Text Articles in Life Sciences
Characterization Of A Stenotrophomonas Maltophilia Bacteriophage, Edward Eshoo
Characterization Of A Stenotrophomonas Maltophilia Bacteriophage, Edward Eshoo
College of Science and Health Theses and Dissertations
Stenotrophomonas maltophilia is a ubiquitous Gram-negative, multidrug resistant, opportunistic bacterial pathogen that causes various infections in humans. Recently, the use of bacteriophages as therapeutic agents, has gained interest as an alternative to traditional antibiotics. This thesis describes the isolation, purification, and characterization of S. maltophilia bacteriophage Bfi2 and discusses its activity against related, and often co-isolated, bacterial pathogens. Amplification of the phage resulted in clear, well-defined plaques and a titer of 1.73 ± 0.38 x 1011 PFU/ml. Bfi2 demonstrated the ability to lyse 55% of the S. maltophilia strains tested, suggesting that it has a moderate host range. However, the …
Evaluation Of Biological Agents For Control Of Macrophomina Root Rot And Powdery Mildew In Flowering Dogwood (Cornus Florida L.), Margaret T. Mmbaga, Lucas M. Mackasmiel, Frank A. Mrema
Evaluation Of Biological Agents For Control Of Macrophomina Root Rot And Powdery Mildew In Flowering Dogwood (Cornus Florida L.), Margaret T. Mmbaga, Lucas M. Mackasmiel, Frank A. Mrema
Agricultural and Environmental Sciences Faculty Research
Six biological control agents (BCAs) (two bacteria, two fungi, and two yeasts) that were previously shown to be effective against powdery mildew (Erysiphe pulchra) were tested for efficacy against Macrophomina phaseolina root rot on flowering dogwood (Cornus florida) in the greenhouse. Two of the bacterial isolates, Stenotrophomonas sp. (B17A) and Serratia sp. (B17B), were effective in controlling both macrophomina root rot and powdery mildew, similar to fungicide control thiophanate methyl, when roots were drenched with the six BCAs individually. In addition, the two bacterial BCAs improved plant growth with respect to stem diameter, stem length, dry weight, and green foliage …
Isolation Of Stenotrophomonas Maltophilia Virus Bfi1: A Biofilm Inhibiting Phage, Thomas Edward Moran
Isolation Of Stenotrophomonas Maltophilia Virus Bfi1: A Biofilm Inhibiting Phage, Thomas Edward Moran
College of Science and Health Theses and Dissertations
The bacterium Stenotrophomonas maltophilia is an emerging infectious pathogen of global concern. Due to its drug-resistant nature, there are limited treatment options available. A potential option for combating S. maltophilia infections is phage therapy, the medicinal use of viruses to treat bacterial infections. Stenotrophomonas phage Bfi1 was isolated from a soil sample using S. maltophilia clinical strain S18202. Transmission electron microscopy provided evidence that this phage is a member of the Siphoviridae family. Host range analysis showed that the phage successfully infected and lysed 30% of the S. maltophilia strains tested. Genomic analysis revealed that the phage contains approximately 32.2-56.5 …
Investigating The Viability Of Two Stenotrophomonas Maltophilia Isolates After Air-Drying, Samantha Lane, Joanna Brooke
Investigating The Viability Of Two Stenotrophomonas Maltophilia Isolates After Air-Drying, Samantha Lane, Joanna Brooke
DePaul Discoveries
Abstract
Stenotrophomonas maltophilia is a water-borne infectious bacterium that is found in both clinical (hospitals) and non-clinical environments. This human pathogen is commonly recovered from respiratory tract infections. A recent study at a hospital in Taiwan suggested that dry patient charts can serve as a vehicle of transmission of this bacterium7. As S. maltophilia is not commonly isolated from dry surfaces, this current study tested the hypothesis that this pathogen can remain viable for some time on a dry surface. This study was designed to determine how long S. maltophilia could remain viable after air-drying by observing …