Life Sciences Commons^™

A Comparison Of Photosynthetic Rate, Stomatal Conductance, And 
Electron Transport Rate Between Malosma Laurina And Encelia Californica, Yujin Choi, Isabella Armstrong, Catherina Haas, Micah Kim

Featured Research

In the Santa Monica Mountains, there are two main vegetation types: chaparral, which makes up __% of plants in these mountains, and coastal sagebrush, which makes up __%. Despite California’s acute environmental stress, both of these plants manage to survive, and even thrive in some cases. We were interested in seeing if in spite of these vast anatomical and physiological differences, these plants were able to survive at similar health rates, measured in photosynthetic rate, stomatal conductance, and electron transport rate (ETR). We have decided to choose 2 plants from 2 different leaf types of different vegetation types: Malosma laurina …

How Soil Salinity Affects The Stomatal Conductance Of Baccharis Salicifolia (Mulefat/Seepwillow), Andrew Kim, Caroline Wilkerson, Mahala Bayless, Peter Du

Featured Research

This is a poster presentation demonstrating the project our group has been working on. We hypothesize that the stomatal conductance of mulefat will decrease as soil salinity increases in both pre-rain and post-rain conditions because the plant tries to absorb salt water. However, the salt water cannot pass through plant tissue and draws water out of the plant. Since plant requires water for photosynthesis, water being drawn out means less water is available for the plant to do photosynthesis. We have tested our hypothesis by collecting soil samples in Legacy park, and recorded stomatal conductance by using a leaf porometer

Comparison Of Photosynthesis, Stomatal Conductance, And Water Potential Between Native Rhus Integrifolia And Invasive Nicotiana Glauca, Tiffany J. Egbo, Emma J. Navarro, Malaun A. Phillips, Cameron I. Zohary

Featured Research

Invasive species, such as Nicotiana glauca, are a prominent part of the Santa Monica Mountains, and could be responsible for displacing native species such as Rhus integrifolia. It was observed in the field that Nicotiana glauca was doing better than Rhus integrifolia, so our group decided to indirectly test this. Because of the invasiveness of its species, it is essential to measure the health of a native species to see if the invasive species is posing as a massive threat. Water potential, stomatal conductance, fluorescence, photosynthetic rates and electron transport rates were compared among the two plants …

Prenylation In The Moss Physcomitrella Patens, Noela Botaka, Susana Perez-Martinez, Liang Bao, Parul Singh, Mark Running

Posters-at-the-Capitol

Protein prenylation is a post-translational modification that involves the addition of lipid groups to the end of a target protein and is necessary for protein activity. Prenylation has important roles in the cell: targeting and localizing proteins to subcellular compartments and promoting protein-protein interactions. Recently, we have found Protein Prenyltransferase Alpha Subunit-like (PPAL), which shares structural similarities to known prenylation enzymes. However, the biochemical function of PPAL is still unknown. PPAL is present in a single copy in other plants examined to date but is present in two copies in moss. Knockouts in our lab of either PpPAL1 or PpPAL2 …

A Crispr Platform For Rapid And Inducible Genome Editing In Human Non-Small Cell Lung Cancer Cells, Lloyd Bartley

Posters-at-the-Capitol

Non-small cell lung cancer (NSCLC) accounts for about 85% of lung cancer, which is the leading cause of cancer death in the world. High mortality rate associated with NSCLC is partially attributed to the limited understanding of NSCLC as well as ineffective therapeutic treatments. The initiation and progression of NSCLC involves genetic changes leading to alterations in the control of tissue development and homeostatic maintenance. Better knowledge about these genetic abnormalities is imperative for developing new chemotherapeutic drugs for NSCLC. Recent research demonstrates that the expression of paraoxonase 2 (PON2), a lactonase/arylesterase with anti-oxidant properties, are markedly enhanced in cancer …

Soil Responses To One-Season Winter Cover Crops, Mckenna Morgan, Iin Handayani

Posters-at-the-Capitol

Soil Responses to One-Season Winter Cover Crops

McKenna Morgan and I. P. Handayani

Hutson School of Agriculture Murray State University, KY, USA

Abstract

In regions like western Kentucky where wooded lots are common there is a growing interest in how to utilize them so that land profits can be maximized. The frequently poor soils in wooded areas due to erosion and lack of ground cover vegetation often cause complications in the creation of pasture areas. Therefore, the objective of this study was to assess the effect of growing winter cover crops on soils to prepare for pasture systems in the …

Consumption Profiles Of Illicit And Neuropsychiatric Drugs In Urban And Rural Communities In Kentucky Using Sewage Epidemiology, Tara Croft, Katelyn Foppe, Rhiannon Huffines, Bikram Subedi

Posters-at-the-Capitol

The drug overdose deaths in the USA increased by ~20% from 2015 to 2016 to the total >63,600 drug overdose deaths in 2016. Kentucky is among the highest rate of drug overdose death states in the USA. The current estimates of the prevalence of substance abuse are based on the self-reported surveys, overdose/toxicological reports, and drug-related crime statistics. Survey-based conventional approaches are not only cost and time-intensive but also underestimate the actual consumption of drugs. As “wastewater never lie,” the drug residues in raw wastewater collected from the centralized wastewater treatment plants were utilized to determine the consumption rate of …

Impact Of Aerobic Compost Tea On Growth Of Lactuca Sativa Within A Peat-Based Growing Media, Ella Potts, Jessica Stoner, Alyx Shultz, Iin Handayani

Posters-at-the-Capitol

Compost and compost tea have been shown in practice to be valuable soil amendments across many horticultural applications; however, little rigorous quantitative work has been completed. Researchers designed a side-by-side trial with two replications to quantifiably study the impact of compost tea on the growth and vigor of soil-grown lettuce. A recycled poultry-litter-based aerobic compost tea was used for the treatment. Six lactuca sativa plants received each treatment or control. Researchers hypothesized the treatment plants would show more vigor and vegetative growth than control. The null hypothesis was treatment plants would be identical to control lettuce. Researchers failed to reject …

Development Of A Pd-L1 Pet Imaging Biomarker, Caleb Jack Bridgwater

Posters-at-the-Capitol

Immunotherapy strategies are very promising treatments for cancer patients. Specifically, Immune checkpoint inhibitor therapy focusing on the PD-1/PD-L1 pathway shows long-lasting positive results in many cancer patients. Unfortunately, not all the patients can benefit from this highly effective treatment. Hence, there is a great need for predictive biomarkers. Immunohistochemical (IHC) staining has been used as a way of predicting patient response, yet shows many problems. For example, IHC utilizes an invasive biopsy and sample fixing, which creates an incomplete and delayed picture of the patient’s biochemistry and the tumor microenvironment, consequently ignoring metastases.

The purpose of this study is to …

Histone Deacetylation Is The Primary Epigenetic Mechanism For Silencing Of Tumor Suppressor Gene - Tissue Factor Pathway Inhibitor-2 In Hepatocellular Carcinoma Cells, Alisha Deshmukh, Shirish Barve

Posters-at-the-Capitol

Hepatocellular carcinoma (HCC) is the third leading cause of cancer related mortality worldwide. With a survival rate of less than 5 percent, a therapeutic treatment is desperately needed to manage this disease. Many epigenetic mechanisms that underlay HCC are being identified. A frequently silenced pathway tissue factor pathway inhibitor-2 (TFPI-2) is a critical tumor suppressor gene. In HCC, inactivation of TFPI-2 leads to tumor growth. Recent research indicated Fas L plays a major role in apoptosis as part of HCC. For the purposes of this study, the phytochemical Curcumin was explored to observe its possible effects on the epigenetic mechanisms …

Effects Of Evolution On Laboratory Sublines Of Myxococcus Xanthus Dk1622, Mackenzie Ryan, Francesca Scribano, Kimberly Murphy

Celebration of Learning

Microbes have served as effective models for studying evolution because of their ability to be easily replicated, stored, and manipulated. Myxococcus xanthus is a soil bacterium that has served as a model organism in many laboratories. The unique social and motile behaviors exhibited by this bacterium make it ideal for phenotypic assays. A wild-type strain of M. xanthus, DK1622, has been distributed to laboratories across the United States and therefore we now have DK1622 sublines. The genomes of a number of these sublines have been sequenced and their social and motile phenotypes have been analyzed. When nine of these sublines …

Identification Of Microbiota Associated With The Ectosymbiotic Community Structure Of The Appalachian Brook Crayfish (Cambarus Bartonii), Matthew M. Cooke 6268927, Luke T. Fischer, Taylor Griffin, Sherrie Jeffers

Montview Journal of Research & Scholarship

Microbes play a significant role in ecosystems, effecting the vital functions of other organisms, interspecies relationships, and population sizes. Based upon previous research (Skelton et al., 2016), it has been hypothesized that crayfish symbionts (branchiobdellidans) play a significant role in regulating the amount of microbiota associated with the host. A growing body of knowledge is known regarding both the condition of gill fouling and the annelid worm’s symbiotic relationship with the crayfish (Skelton et al., 2016; Skelton et al., 2016). However, there is little to no evidence available concerning the bacteria’s role in the process (Skelton et al., 2016). Microbial …

A Student’S Perspective On Engagement In Ecological Research During Their First Semester In College, Natalie M. Aguirre, Karagan L. Smith, Cristian M. Garcia, Cheryl Swift, Stephen D. Davis

Biology

Pepperdine University and Whittier College jointly initiated a new first-year seminar series in the fall of 2016 that engaged students in authentic research, their first semester on campus. The goals are listed below. Assessment of these goals included both qualitative focus groups and quantitative survey questionnaires administered by an outside assessment agency, WestEd.org.

Ninety-two percent of students reported that their first-year seminar improved learning in concurrent courses and ninety to ninety-three percent of students said their first-year seminar increased their interest in STEM. In addition, students self-reported a significant improvement in all aspects of their ability to plan and conduct …

Post Drought Recovery In Malosma Laurina After Severe Dieback During California’S Historic Drought Of 2012-2016, Karagan L. Smith, Guinevere X. Mesh, Natalie M. Aguirre, Gabriella N. Palmeri, Helen I. Holmlund, Stephen D. Davis

Biology

Malosma laurina is a species of chaparral shrub that dominates coastal exposures of the Santa Monica Mountains of southern California. Between 2012 and 2016, protracted drought with half normal rainfall over a five-year period (175 mm), led to severe dieback, exceeding 50% whole plant mortality at some sites. The drought dissipated in 2017 with an annual rainfall that exceeded the 140-yr normal by 108 mm. As a result of this elevated annual rainfall in 2017, we were interested in documenting the potential for full recovery of Malosma laurina as a keystone species of coastal chaparral shrub communities. We tested the …

Introduction To Integrated Crop-Livestock Systems, P. Troy White

STEM & Agricultural Education Curriculum Resources

This presentation is an introductory lesson on integration of crop-livestock systems in the United States. The lesson includes national Agriculture, Food, and Natural Resources Standards, objectives, and a student project. This lesson is intended to be inquiry-based, and as such only foundational information is presented. Students are expected to take the introductory information and fins specific information needed to sold the problem posed at the beginning of the presentation. It is expected that at a minimum this lesson will take three 50-minute class periods and could take longer based on teacher preferences.

Effects Of Environmental Enrichment On Behavior In A Domestic Goat Herd, Stephanie Peramas

Senior Honors Projects

Animal welfare is one of the most important parts of animal management. Apart from other measures of good animal welfare, like being in good health, animal behavior can be indicative of the animal’s internal state and is often one of the first signs of improper welfare. Stereotypies, or stereotypic behavior, are commonly viewed as a symptom of improper animal welfare. Unenriched, or barren, environments associated with farmed livestock have long been associated with inducing stereotypic behavior. The addition of precise enrichment to the animals’ environment is a common remedy for stereotypic behavior. With respect to stereotypic goat behavior, few empirical …

Improving Habitat Restoration For Native Pollinators In San Francisco, Tyrha Delger

Creative Activity and Research Day - CARD

By looking at how these three species are conserved inside and outside of San Francisco, how an urban environment might affect habitat conservation, and lessons learned from other cities’ restoration projects, we can improve urban habitat conservation projects.

Urban Biogeography Of Fungal Endophytes In Metrosideros Excelsa Across San Francisco, Emma Gibson

Creative Activity and Research Day - CARD

Trees in cities confer health and psychological benefits to the city's inhabitants, so the urban forest is a major component of human health in urban settings. Previous studies have shown that environmental factors can impact endophytic (within-plant) microbial communities of forest trees, but the effects that urban environmental factors may have on these communities are not well understood. Here, we characterized asymptomatic fungal communities in 30 individual Metrosideros excelsa trees from 6 distinct sites using culturing and molecular methods. We found high community diversity both within and among sites, and several patterns within these communities. For example, we found similarities …

Plant Biodiversity In The Face Of Climate Change: What Will Become Of Coastal Endemic Species?, Nila Le

Creative Activity and Research Day - CARD

How will longitudinally constrained coastal flora fare in the face of rapid anthropogenic climate change and disturbance? Coastal communities pose a unique problem for climate change and its associated risks. Species that are concentrated along the coast are naturally vulnerable to the influence of harsh abiotic ecology, such as coastal erosion and high salt concentrations. In addition, analogous to montane species-habitat interactions, coastal communities may be “pushed” off the coast edge as a result of tracking preferred climate in the face of climate variability and sea level rise. This can be problematic for species that cannot disperse quickly enough to …

Bioaccumulation Of Zinc In Bermuda Grass (Cynodon Dactylon), Drew Fisher, H. Marie Surratt, Louise Gebauer, Andrew Palaski

Featured Research

Phytoremediation is the plant based process to remove toxins such as heavy metals from the soil. In this experiment, we hypothesized Bermuda grass (Cyndon dactylon) will absorb a detectable level of zinc, a nontoxic metal, from the soil. We grew two separate groups of grass: the control and the experimental, which was watered with zinc acetate. Neither of the groups produced enough yield within the weeks given to the experiment, but if it went according to plan, we would have used flame atomic absorption spectroscopy to evaluate the relative concentrations of zinc. Significant uptake of zinc could reveal a possible …

The Effects Of Soil Suffused With Cool-Fire Ash On The Germination And Early Growth Of Radish Seedlings, Ryan Dalforno, Braden Ritchey, Jay Ogle

Featured Research

In this study, the effects of cool fire ash in soil with varying amounts of water were observed in terms of the germination and early growth of garden radish (Raphanus raphanistrum subsp. sativus) seedlings. Measurements regarding plant growth, field capacity, and seedling leaf quantum yield were taken and compared between four (4) trios of pots separately containing radish seedlings planted in ashy and frequently-watered soil (A_w), ashy and infrequently-watered soil (A_d), normal unmodified garden soil that was watered frequently (N_w) and normal soil that was watered infrequently (N_d). While the …

A Comparison Of Electron Transport Rate, Photosynthetically Active Radiation, Light-Adapted Fluorescence, And Dark-Adapted Fluorescence Between Stipa Pulchra And Pennisetium Setaceum, Sydney Adams, Brandon Chuck, Agatha Heng, Amelia Vento, Stephen D. Davis

Featured Research

The purpose of this investigation was to provide an explanation into how the invasive species Pennisetium setaceum, Fountain Grass, is able to outcompete the native California grassland species Stipa pulchra, Purple Needle Grass. We used a light adapted and a dark adapted fluorometer to measure the photosynthetic radiation (PAR), leaf temperature, alpha (α), light adapted fluorescence (Fv’/Fm’), dark adapted fluorescence (Fv/Fm), and the electron transport rate (ETR) on young Fountain Grass and Purple Needlegrass. After collecting and analyzing the data, we concluded that the dark adapted fluorescence (Fv/Fm) was the only statistically significant measurement where the Fv/Fm of …

Emergence Of Zoos In The U.S. And A Search For The Exotic, Austin M. Hutchinson

Student Scholar Showcase

The emergence of zoos in twentieth century America had many factors that contributed to their mission, success, and overall exotic nature. Zoos have been a part of human civilization for centuries, but it would not be until 1876 when the first zoo would appear in the United States. Individuals craved excitement and the use of exotic animals from all over the world became an important component of what zoos would become. This era coincides with the European colonization of Sub-Saharan Africa, known as the Scramble for Africa. Animals were brought from the so-called “Dark Continent” to be put on display, …

Sirna Knock-Down Of Vav1 In Breast Cancer Cells: Effects On Pi3kinase And Akt, Tajai Sion-Milligan

Student Scholar Showcase

One in eight women in the United States will be diagnosed with breast cancer. Approximately 25-30% of these breast tumors will overexpress Human Epidermal Growth Factor Receptor 2 (HER2), associated with a worse prognosis and decreased survival rate. Stimulation of HER2 results in activation and production of many signaling molecules, ultimately resulting in increased cell division. Two of the downstream molecules activated are PI₃Kinase and, further downstream, the molecule Akt. Typically, overproduction and/or overactivation of PI₃Kinase can lead to oncogenesis. A molecule that may have a significant role in PI₃Kinase formation is VAV1. VAV1 …

Creating A Predictive Model For Flowering Of Virginia Orchid, Cypripedium Pubescens, Emily Horton

Student Scholar Showcase

No abstract provided.

Evaluating Effects Of Gene Mutation And Light Intensity On Arabidopsis Thaliana Development, Carolanne Bekus

Student Scholar Showcase

The completion of sequencing Arabidopsis thaliana’s genome has led to the next crucial challenge of determining gene function in these plants. A. thaliana is a model organism often compared to commercial crops. The discovery of gene function within these plants will aid in giving insight on how gene function can affect commercial crop production. Presented here is an analysis comparing wild-type Columbia (Col-O), to single gene mutants when knocking-out transcriptional factors VPI/ABI3-like 1 (val1) and basic region/leucine zipper motif (bZIP). These single gene mutations may affect several traits that in turn can result in morphological changes and/or …

Analysis Of Downstream Signaling Of Tlrs After U0126 Inhibition, Brandy Frazier

Student Scholar Showcase

Francisella tularensis (F. tularensis) is a pathogenic bacterium which causes the disease tularemia. F. tularensis is thought to be able to enter and multiply inside of liver cells because it interacts with toll-like receptors (TLRs). Examining the signaling pathway downstream of the TLRs will allow for better understanding of how this disease works and possible treatment strategies. In this study I propose to examine how molecular inhibitors affect signaling downstream of TLRs in HepG2 cells treated with lipopolysaccharides (LPS) from E. coli. Mitogen-activated protein kinase is a cellular molecule in signaling cascades that directs cellular response mechanisms. MAPK can be …

Eating Crickets? Nutrient Mineral Content Of Two Cricket Species And Chapul Cricket Protein Bars Using Mp-Aes, Nathan Hopkins

Student Scholar Showcase

Global agriculture is beset by numerous challenges which must be surmounted to feed a developing world, from combating climate change to the cultivation of sustainable food products. This, in addition to protein and nutrient deficiencies in the developing world, makes considering alternative protein sources, such as a crickets, an attractive option. Crickets use a fraction of the land and water that protein sources like beef cattle do, and can be cultivated in urban environments in stacked boxes. This research investigation aims to quantify the nutrient mineral content (Iron, Sodium, Potassium, Calcium, and Magnesium) of two cricket species (Acheta domesticus …

Elemental Analysis Of Beer Manufactured In Virginia Satellite Breweries, Jennifer Wiliams

Student Scholar Showcase

Financial incentives from the state and wider distribution potential have lured many west coast brewers to open satellite breweries in Virginia. Quality control is crucial for any brewery and especially important in satellite breweries that need to imitate the standard set by the parent brewery. Zinc, magnesium, iron, sodium manganese, and calcium are trace elements found in wort and are essential for enzymes that catalyze fermentation reactions. These elements protect against cell stress, play a crucial role in flocculation, and can affect the flavoring and coloration of beer. Barley, water, and hops are the sources of these elements in beer. …

Discovery Of Geographical Gene Variants In Related Pseudomonas Aeruginosa Bacteriophages, Emilee Carr, Elise Melhado, Emily Loertscher, Trever Thurgood, Ruchira Sharma, Julianne H. Grose

Library/Life Sciences Undergraduate Poster Competition 2019

Antibiotic resistant bacterial strains are a major crisis in the world due to the difficult nature of curing individuals afflicted with them. Phage therapy has been proposed as an alternate treatment for these bacterium. In Dr. Julianne Grose's lab, bacteriophages were against the bacteria, Pseudomonas aeruginosa. To isolate, environmental samples were utilized in enrichment cultures that were ultimately used in serial dilutions, plaque purification, electron microscopy, DNA isolation, sequencing, and genome annotation. The P. aeruginosa phage, TF17, infects a bacterial strain that is highly related to a strain that causes fatalities as an opportunistic infection in patients with cystic fibrosis. …