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Articles 31 - 60 of 122
Full-Text Articles in Life Sciences
Combination Treatment With Conjugated Linoleic Acid And Nitrite Protects Against Myocaridal Infarction., Natia Qipshidze Kelm
Combination Treatment With Conjugated Linoleic Acid And Nitrite Protects Against Myocaridal Infarction., Natia Qipshidze Kelm
Electronic Theses and Dissertations
According to the CDC, the most common type of heart disease is coronary artery disease, which frequently leads to myocardial infarction (MI). Therapeutic approaches to lessen the resulting cardiovascular injury associated with MI are limited. Recently, the management paradigm for cardiac injury has entered the molecular era and microRNAs (miRNAs) have been shown to act as negative regulators of gene expression by inhibiting mRNA translation and/or stimulating mRNA degradation. A single miRNA can modulate physiological or disease phenotypes by regulating whole functional systems. Importantly, miRNAs can regulate cardiac function, thereby modulating heart muscle contraction, heart growth and morphogenesis. MicroRNA-499 (miRNA-499) …
Skeletal Muscle Lipid Metabolism And Markers Of Insulin Resistance In Young Male Low Birth Weight Offspring In Combination With A Postnatal Western Diet, Kristyn Pamela Dunlop
Skeletal Muscle Lipid Metabolism And Markers Of Insulin Resistance In Young Male Low Birth Weight Offspring In Combination With A Postnatal Western Diet, Kristyn Pamela Dunlop
Electronic Thesis and Dissertation Repository
Low birth weight offspring are at increased risk for developing metabolic syndrome in later life, specifically its precursor, insulin resistance (IR). Reduced mitochondrial lipid metabolism is implicated in IR pathogenesis, promoting accumulation of acylcarnitines, and potentiating alterations in phosphorylation status of insulin signaling intermediates. While high-energy Western diets are classically implicated in IR progression, the in utero environment was recently highlighted as a major programming mechanism of later life IR. Using a guinea pig model of placental insufficiency, we investigated how an adverse in utero environment impacts later life mitochondrial lipid metabolism and IR progression, as well as its interaction …
Freeze Tolerance In The Spring Field Cricket, Gryllus Veletis, Alexander H. Mckinnon
Freeze Tolerance In The Spring Field Cricket, Gryllus Veletis, Alexander H. Mckinnon
Electronic Thesis and Dissertation Repository
Many insects are able to survive internal ice formation. However, the mechanisms underlying freeze tolerance are not well-understood, perhaps because of a lack of suitable model organisms. I found that the spring field cricket, Gryllus veletis, seasonally acquires freeze tolerance in the fall when kept outside in London, Ontario. Moreover, individuals acquired freeze tolerance in the laboratory in response to a simulated fall thermophotoperiod. Lab-acclimated G. veletis freeze at -6.1 ± 0.7 ºC and the acquisition of freeze tolerance is accompanied by the accumulation of proline and trehalose. Crickets survived temperatures as low as -12 ºC (1.5 h), and …
The Effects Of Ovarian Hormones And Exercise On Gene Markers Of Cardiac Dysfunction, Anisha S. Patel
The Effects Of Ovarian Hormones And Exercise On Gene Markers Of Cardiac Dysfunction, Anisha S. Patel
Masters Theses
Heart disease is the leading cause of death in women in the United States. Premenopausal women appear to have better cardiac function and lower risk of heart disease compared to male postmenopausal female counterparts. Ovarian hormone loss influences blood pressure homeostasis and causes systemic inflammation, which may result in chronic stress on the heart. Two key physiological changes in cardiac dysfunction are reemergence of the fetal gene pattern and myocardial remodeling. Physical activity has been linked to improved cardiac function. The purpose of this study was to investigate the effects of ovariectomy on early markers of cardiac dysfunction and fibrosis …
The Ctcf Chromatin Organizer Is Required For Hindlimb Development, Katherine L. Rabicki
The Ctcf Chromatin Organizer Is Required For Hindlimb Development, Katherine L. Rabicki
Electronic Thesis and Dissertation Repository
Mutations in chromatin organizer CTCF were identified in patients with intellectual disability and skeletal defects. Previous studies demonstrated that depletion of CTCF in murine limb mesenchyme results in apoptosis in the forelimb. The role of CTCF in the hindlimb, however, is unknown. My objective was to investigate effects of CTCF deletion on chondrogenesis and skeletal development in the hindlimb. In vitro wild-type micromass cultures demonstrate that chondrocyte gene expression is delayed in the hindlimb when compared to forelimbs. Embryonic CtcfFl/Fl;Prx1Cre mice were investigated, and qRT-PCR and histology were performed on limb buds and long bones. Results show that …
Thermal Performance Covaries With Environmental Temperature Across Populations Of Atlantic Salmon (Salmo Salar), Kayla J. Harding Gradil
Thermal Performance Covaries With Environmental Temperature Across Populations Of Atlantic Salmon (Salmo Salar), Kayla J. Harding Gradil
Electronic Thesis and Dissertation Repository
Global climate change is projected to have widespread effects that could threaten the viability of natural populations. Physiological processes of aquatic ectotherms critically depend on their thermal environment, such that the optima for performance often correspond to environmental temperatures. Given predicted changes in aquatic thermal environments, it is increasingly important to understand organism’s underlying physiological mechanisms utilized to cope with these changes. Here, I show that three populations of Atlantic salmon (Salmo salar) are narrowly adapted to their native summer temperatures, such that thermal tolerance is optimized near average temperatures and collapses near peak temperatures. Further, I found …
Reactivity Of The Middle Cerebral Artery To Carbon Dioxide, Nicole Coverdale
Reactivity Of The Middle Cerebral Artery To Carbon Dioxide, Nicole Coverdale
Electronic Thesis and Dissertation Repository
Transcranial Doppler ultrasound (TCD) is used for the assessment of cerebral blood flow velocity (CBFV) at the middle cerebral artery (MCA) with the assumption that diameter of the artery does not change. Thus, CBFV is equivalent to cerebral blood flow (CBF). The purpose of this thesis was determine if the MCA dilates during hypercapnia (HC) and/or constricts during hypocapnia (HO) in healthy young and older adults using 3T magnetic resonance imaging (MRI). We also determined how these changes in MCA cross-sectional area (CSA) influence estimates of CBF and cerebrovascular reactivity (CVR) from TCD in young and older adults. Lastly, we …
The Effects Of Arginine Vasopressin On Maternal Behavior And Aggression In Peromyscus Californicus Mothers, Nathaniel Ng
The Effects Of Arginine Vasopressin On Maternal Behavior And Aggression In Peromyscus Californicus Mothers, Nathaniel Ng
Honors Projects
Research studies since the 1950s have shown that a chemical within the brain called arginine vasopressin (AVP) is associated with the modulation of many different social behaviors in mammals. Some of these behaviors are related to parenting, such as parental care initiation, aggression, social recognition, depression and anxiety. Understanding the physiology behind AVP regulation could allow for the creation of new therapies for treating human social disorders, such as using an AVP receptor antagonist to attenuate anxiety. This project examines how neural injections of AVP and an AVP receptor antagonist affect both maternal care and aggression in female Peromyscus californicus …
The Role Of Shroom3 In Kidney Development, Alexandra Sull
The Role Of Shroom3 In Kidney Development, Alexandra Sull
Electronic Thesis and Dissertation Repository
Chronic kidney disease (CKD) affects 10% of the population in industrialized nations. Recent genome wide association studies (GWAS) have correlated SHROOM3 with glomerular filtration rate and albuminuria. To elucidate Shroom3’s role in the kidney, I employed a gene trap mouse model (Shroom3Gt/Gt allele) that produces a non-functional Shroom3 protein. Shroom3 is expressed in the podocytes embryonically and in adulthood as well as other cell types. Shroom3 loss results in glomerular cysts and reduced glomerular number. Some Shroom3+/Gt and Shroom3Gt/Gt podocytes showed loss of apical ROCK1, pMLC and actin consistent with the role of Shroom3 in …
Novel Video Imaging To Examine Cardiac Function In Xenopus Embryos, Judith Sull
Novel Video Imaging To Examine Cardiac Function In Xenopus Embryos, Judith Sull
Electronic Thesis and Dissertation Repository
Congenital heart defects (CHDs) occur in approximately 1% of live births and the etiology has been associated with disturbances in cardiogenesis. However, the majority of research examining CHDs relies on static morphological data, which does not elucidate how defects alter cardiac function. I used Xenopus laevis embryos to examine the association between CHDs and functional alterations using a novel imaging system that can obtain high- resolution images through a non-invasive procedure. A high-speed video camera and software were used to assess cardiac function, permitting functional characterization of late Xenopus cardiogenesis. Verification of the imaging system’s ability to detect changes in …
The Effect Of Circadian Rhythm On Cortisol And Perceived Stress Correlations, Adam Bender
The Effect Of Circadian Rhythm On Cortisol And Perceived Stress Correlations, Adam Bender
Honors Theses
Stress is a known trigger of the Hypothalamus-Pituitary-Adrenal (HPA) Axis which leads to the production and secretion of the catabolic steroid cortisol by the adrenal gland. Since cortisol production is affected by stress, it follows that a high self-perception of stress would be correlated with high blood and saliva cortisol levels. Literature generally shows a gap in identifying this correlation, perhaps because of the interconnected nature of endocrine pathways. New experimental methods that control for the effects of circadian rhythm have shown limited success in demonstrating this correlation. Our purpose is to continue exploring the relationship between cortisol, perceived stress, …
Hmgb1 And Ceramides: Potential Mediators Of Cigarette Smoke-Induced Metabolic Dysfunction, Mikayla Orton Thatcher
Hmgb1 And Ceramides: Potential Mediators Of Cigarette Smoke-Induced Metabolic Dysfunction, Mikayla Orton Thatcher
Theses and Dissertations
While cigarette smoking is a common-knowledge way to stay lean, it has long been known as a risk factor for diabetes and obesity. Here we establish that smoking causes fat gain and metabolic disruption in mice, effects which are exacerbated by a high-fat, high-sugar diet. We found that smoke exposure increases levels of ceramide—the lipid responsible for diet-induced insulin resistance—and that blocking ceramide production with the pharmacological inhibitor myriocin restored insulin sensitivity, stopped weight gain, and rescued mitochondrial respiration in vivo and in vitro.We also sought to assess the impact of the RAGE ligand HMGB1 on skeletal muscle metabolism. We …
Differential Receptors For Advanced Glycation End-Products (Rage) Expression In Preeclampsia, Intrauterine Growth Restriction And Gestational Diabetes, Kristen Lena Alexander
Differential Receptors For Advanced Glycation End-Products (Rage) Expression In Preeclampsia, Intrauterine Growth Restriction And Gestational Diabetes, Kristen Lena Alexander
Theses and Dissertations
Preeclampsia (PE), intrauterine growth restriction (IUGR) and gestational diabetes (GDM) increase the risk of maternal and fetal morbidity and mortality. The roles of Advanced Glycation End-products (AGEs) are already well documented concerning inflammation, hypoxia and oxidative stress. AGEs bind to its receptor, Receptor for Advanced Glycation End-products (RAGE), and activate an inflammatory pathway. This pathway alters the efficacy of invasive trophoblast cells and in the placenta and can result in placental dysfunction. We hypothesized that the placental dysfunction found in PE, IUGR, and GDM resulted from an over activation of the RAGE-mediated inflammatory pathway. Using human placental samples, we found …
The Effects Of Inhibiting Wnt Secretion And Activity On Cranial And Neural Development, Julie Louise Hulet
The Effects Of Inhibiting Wnt Secretion And Activity On Cranial And Neural Development, Julie Louise Hulet
Theses and Dissertations
Wnt signaling has been shown to have several roles in the development of sensory neurons, particularly in the ophthalmic portion of the trigeminal nerve. Many of these studies have relied on the conclusion that Wnt is necessary but not sufficient for the induction and maintenance of the neural precursor cells that develop in the ophthalmic placode. Wnt had been inhibited in the ophthalmic placode using a dominant negative t-cell factor (TCF) and resulted in the loss of Pax3 expression (indicative of undifferentiated placode cells) in all targeted cells, suggesting a loss of specification/commitment of these cells to the sensory neuron …
Cigarette Smoke Increases Cardiomyocyte Ceramide Accumulation And Inhibits Mitochondrial Respiration, Trevor Stanley Tippetts
Cigarette Smoke Increases Cardiomyocyte Ceramide Accumulation And Inhibits Mitochondrial Respiration, Trevor Stanley Tippetts
Theses and Dissertations
Cigarette smoking is a common and lethal worldwide habit, with considerable mortality stemming from its deleterious effects on heart function. While current theories posit altered blood lipids and fibrinogen metabolism as likely mediators, none have explored the role of the sphingolipid ceramide in exacerbating heart function with smoke exposure. Ceramide production is a consequence of cigarette smoke in the lung, and considering ceramide's harmful effects on mitochondrial function, we sought to elucidate the role of ceramide in mediating smoke-induced altered heart mitochondrial respiration. Lung cells were exposed to cigarette smoke extract and heart cells were exposed to the lung-cell conditioned …
The Effect Of Run Sprint Interval Training On Diabetic Metabolic Markers In Prediabetic Adults, Kathryn L. Hilovsky
The Effect Of Run Sprint Interval Training On Diabetic Metabolic Markers In Prediabetic Adults, Kathryn L. Hilovsky
Masters Theses, 2010-2019
Abstract
Purpose: The primary purpose of this study was to compare the effects of an 8-week run-sprint interval training (R-SIT) and continuous moderate-intensity training (MIT) on fasting blood glucose, HbA1c, insulin sensitivity, and β-cell function in sedentary, prediabetic adults. Secondary outcomes of the study included anthropometric, body composition variables, and aerobic capacity.
Methods: Sedentary, physician diagnosed prediabetic individuals were randomized into R-SIT (n=7, BMI 36.76 ± 9.79) or MIT (n=8, BMI 40.59 ± 12.49) interventions. Subjects participated in supervised exercise three times a week and attended a Diabetes Prevention Program course, once weekly. R-SIT participants performed 4-6 x 30-second …
The Effect Of Taste On Swallowing Function, Rachel Mulheren
The Effect Of Taste On Swallowing Function, Rachel Mulheren
Dissertations, 2014-2019
This study investigated the effects of taste on swallowing frequency and cortical activation in the swallowing network. The effects of salivary flow and taster status were also examined, along with genetic taster status. The effects of a 3ml bolus compared sour, sour with slow infusion, sweet, water, and water with infusion. Swallowing frequency was significantly higher 0-15 seconds after bolus delivery than 16-30 seconds. Swallowing frequency was higher in the sour conditions, whereas sweet and water did not differ. Functional near-infrared spectroscopy recordings measured changes in blood oxygenation (HbO) in the right and left hemispheres in the premotor, S1 and …
Effects Of The Developmental Environment On Oxidative Damage And Antioxidants In Red-Eared Slider Turtle ( Trachemys Scripta Elegans ) Hatchlings, Lisa Allison Treidel
Effects Of The Developmental Environment On Oxidative Damage And Antioxidants In Red-Eared Slider Turtle ( Trachemys Scripta Elegans ) Hatchlings, Lisa Allison Treidel
Theses and Dissertations
Developmental environments influence many individual phenotypes. However, currently we have a limited understanding of how the developmental environment influences oxidative stress resistance phenotypes. Oxidative stress is defined as a physiological state during which the production of harmful free radicals exceeds the protective capabilities of antioxidants. Variations among adults in susceptibility to oxidative stress can have important consequences for life history strategies and fitness related traits. Our work was aimed at providing insight in to the role that the developmental environment plays on oxidative stress phenotypes in the model oviparous reptile the red-eared slider turtle (Trachemys scripta elegans). Here, we conducted …
Functional Significance Of Gill Claudin Proteins In Rainbow Trout (Oncorhynchus Mykiss) Osmoregulation, Joanna Katarzyna Bujak
Functional Significance Of Gill Claudin Proteins In Rainbow Trout (Oncorhynchus Mykiss) Osmoregulation, Joanna Katarzyna Bujak
Graduate Theses and Dissertations
Claudin proteins, a key element of tight junction complexes, are known to control paracellular permeability. In euryhaline fish, changes in claudin abundance and localization are critical during salinity acclimation. In seawater, a leaky paracellular pathway that facilitates sodium extrusion is hypothesized to be controlled by claudin proteins. The aim of this study was to evaluate the role of Claudin-10c, -10d -10e and Claudin-30 in gill function in freshwater (FW) and seawater (SW) rainbow trout (Oncorhynchus mykiss). I examined mRNA and protein abundance along with cellular localization. A tissue distribution survey showed that all of the claudins studied were predominantly expressed …
Effects Of Pre-Release Physical Stressors On Post-Release Success Of Hatchery-Reared Spotted Seatrout, Taylor Westbrook Guest
Effects Of Pre-Release Physical Stressors On Post-Release Success Of Hatchery-Reared Spotted Seatrout, Taylor Westbrook Guest
Master's Theses
Alteration of habitat associated with coastal development and increased demand for food and recreation can result in the depletion of fisheries resources such as the Spotted Seatrout (Cynoscion nebulosus), the Gulf of Mexico’s most popular recreational fish. Stock enhancement, or the release of cultured fish to supplement wild populations, is one potential tool for managing important fisheries resources. Although much effort has gone into marine stock enhancement, the effectiveness of stocking is not well established, and techniques for ensuring success have not been developed. Although the basic biology of the Spotted Seatrout is well known, comparatively little is known about …
The Reproductive Ecology Of The Timber Rattlesnake, Crotalus Horridus, In Northwestern Arkansas: Interactions Between Environment, Steroid Hormones, And Life History, Craig Michael Lind
The Reproductive Ecology Of The Timber Rattlesnake, Crotalus Horridus, In Northwestern Arkansas: Interactions Between Environment, Steroid Hormones, And Life History, Craig Michael Lind
Graduate Theses and Dissertations
In this dissertation I examined the relationship between individual energetic status, hormone production, and life history trait expression in field-active Timber Rattlesnakes, Crotalus horridus. In chapter one I reviewed what is known regarding these relationships in snakes and defined major research goals. In chapter two I described the seasonal profile of testosterone (T) and corticosterone (CORT) in relation to the breeding season and to individual energetic status in males. Results showed that the seasonal pattern of T production in C. horridus was different than other pit viper species with similar mating patterns. Testosterone was elevated in the months leading up …
An Investigation Of Aerobic Fitness, Perceived Quality Of Life And The Direct Effects Of Self-Determination Theory In College Students 2015, Alanna Darling
An Investigation Of Aerobic Fitness, Perceived Quality Of Life And The Direct Effects Of Self-Determination Theory In College Students 2015, Alanna Darling
Master's Theses
There have been numerous studies examining the association between physical activity, motivation and quality of life. In contrast, studies focusing on aerobic fitness and its relationship with levels of motivation and perceived quality of life are lacking. The purpose of this study was to explore the relationship between a direct measure of aerobic fitness, behavioral regulations, and perceptions of quality of life in college-aged sample. Participants included both males (n=129; 21.1±1.8 years) and females (n=89; 20.8±1.4 years) who completed the Behavioral Regulations of Exercise Questionnaire-2 sub-scores and Perceived Quality of Life (PQoL) instruments. Participants then performed the Queen’s College Step …
The Effects Of A Four-Week Whole-Body High-Intensity Training Program On Female Athletes 2015, Lindsey Taylor
The Effects Of A Four-Week Whole-Body High-Intensity Training Program On Female Athletes 2015, Lindsey Taylor
Master's Theses
Purpose: The purpose of this study was to examine the aerobic benefits and changes in running economy (RE) following a fourweek whole-body high-intensity interval training (HIIT) program on an athletic female population. Participants: Fourteen female student-athletes volunteered to participate in the study. Design: Participants reported on two occasions for VO2max and RE testing and were divided into two training groups based upon baseline VO2max: Endurance (END; n=6) and HIIT (n=8). Participants completed 12 total training sessions. During each session, END completed 30 minutes of vigorous-intensity running, while HIIT completed a total of four minutes of whole-body intervals. Measures: Maximal oxygen …
Differential Activation Of Microglia In An In Vitro Model Of Intracerebral Hemorrhage, Bhakti Patel
Differential Activation Of Microglia In An In Vitro Model Of Intracerebral Hemorrhage, Bhakti Patel
Honors Capstone Projects - All
An in vitro model of intracerebral hemorrhage was established to examine the protective versus cytotoxic roles of microglia in the context of mild versus severe injury. Co-cultures of microglia, astrocytes, and granule neurons were prepared from the cerebellar cortex of neonatal rats, and grown in standard medium containing fetal bovine serum or, in some cases, a serum-free chemically defined medium. To mimic hemorrhagic stroke, co-cultures grown for 7-8 days in vitro were challenged with two different concentrations of the toxic blood product hemin, corresponding to a mild versus a severe brain bleed. Immunocyto-chemical, real-time RT-PCR, iron deposition, and cell survival …
Effects Of Thigh Compression Wraps On Sprint Performance In A Physically Active Population 2015, Kevin Ostempowski
Effects Of Thigh Compression Wraps On Sprint Performance In A Physically Active Population 2015, Kevin Ostempowski
Master's Theses
Compression garments are widely used in sport to enhance performance. The purpose of this study was to examine the effect of thigh compression wraps on 60 m sprint performance. Twenty-six physically active college students, seven men and nineteen women, were participants in the study. The participants completed three 60 m sprints in each of two test sessions. The second test session occurred two to seven days after the first test session. The participant’s thighs were compression wrapped in one test session, the treatment session, and not wrapped in the other test session, the control session. Whether or not the first …
Thrombospondin-1 Decreases No-Mediated Vasodilation In Coronary Arterioles In Advancing Age., Grant Mckenzie
Thrombospondin-1 Decreases No-Mediated Vasodilation In Coronary Arterioles In Advancing Age., Grant Mckenzie
College of Arts & Sciences Senior Honors Theses
No abstract provided.
The Characterization Of Amyloid Fibrils And Novel Synthetic Heparin-Binding Peptides Binding To Cell Surfaces, Nicole Marie Hackenbrack
The Characterization Of Amyloid Fibrils And Novel Synthetic Heparin-Binding Peptides Binding To Cell Surfaces, Nicole Marie Hackenbrack
Chancellor’s Honors Program Projects
No abstract provided.
Oral Vs. Nasal Breathing During Submaximal Aerobic Exercise, Chase Ovila Platt Lacomb
Oral Vs. Nasal Breathing During Submaximal Aerobic Exercise, Chase Ovila Platt Lacomb
UNLV Theses, Dissertations, Professional Papers, and Capstones
When comparing oral breathing versus nasal breathing more volume of air can be transported through the oral passageway, but nasal breathing can lead to slower respiration rates and cleaner inspired air. The purpose of this study is to find the most efficient mode of breathing during different intensities of submaximal aerobic exercise. There were 9 males and 10 females that completed this study. First test was a VO2 Max test, 3.0 mph for 3 minutes, with increases in 1.0 mph every minute after that. Using a regression equation running speeds were determined for each individual’s submaximal intensities. The desire …
Pulmonary Diffusing Capacity Is Unaltered In Elite Swimmers After Restricted Breathing Training., Benjamin Todd Ogle 1990-
Pulmonary Diffusing Capacity Is Unaltered In Elite Swimmers After Restricted Breathing Training., Benjamin Todd Ogle 1990-
Electronic Theses and Dissertations
Controlled frequency breath (CFB) holding is a swim training modality that involves holding one’s breath for ~12 strokes before taking another breath. We looked to examine the effects of CFB training on pulmonary diffusing capacity for nitric oxide (DLNO) and carbon monoxide (DLCO). Elite swimmers (n = 25) were divided into either the CFB or a group that breathed regularly, every ~3rd stroke. The training intervention included 16 sessions of 12 x 50-m repetitions with either breathing pattern. Approximately 60% of the males and ~20% of the females were above the upper limits of normal for diffusing capacity at baseline. …
Outline Plan For A Student-Involved Fitness Assessment Program At Western Michigan University, Joanna Gangwisch
Outline Plan For A Student-Involved Fitness Assessment Program At Western Michigan University, Joanna Gangwisch
Honors Theses
This research was aimed at developing a fitness assessment program that would provide undergraduate exercise science students at Western Michigan University (WMU) with hands on experience that would successfully prepare them for their internships and future careers as well as benefiting the campus community at large. Data for this research was obtained by examining the student recreation center websites and sending out an email/phone questionnaire to WMU’s 10 peer institutions. Direct comparisons were made and evaluated. On the basis of the results of this research, it can be concluded that WMU’s exercise science department and student recreation center together could …