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Full-Text Articles in Manufacturing

Laser-Induced Forward Transfer (Lift) Based Bioprinting Of The Collagen I With Retina Photoreceptor Cells, Md Shakil Arman Aug 2021

Laser-Induced Forward Transfer (Lift) Based Bioprinting Of The Collagen I With Retina Photoreceptor Cells, Md Shakil Arman

Theses and Dissertations

This thesis focuses on the 3D bioprinting of retina cone cells using the laser-induced forward transfer (LIFT) technique. Bioprinting has a great potential to mimic and regenerate the human organoid system, and the LIFT technique has emerged as an efficient method for high resolution micropatterning and microfabrication of biomaterials and cells due to its capability of creating precise, controlled microdroplets. In this research, the parameters for an effective femtosecond laser-based LIFT process for 3D bioprinting of collagen biomaterial were studied. Different concentrations of collagen I solutions were tested to find out the right concentration that can be transferred through the …


Investigation Of Cell Behavior In 3d Printed Lumen Structures For Capillary Regeneration, Victoria Jade Perez Aug 2021

Investigation Of Cell Behavior In 3d Printed Lumen Structures For Capillary Regeneration, Victoria Jade Perez

Theses and Dissertations

This thesis work successfully generated a coaxial printing setup and process to generate 3D printed tubules. A dual syringe pump mechanism was developed to print tubular structures to investigate cell behavior with a goal to generate future capillary beds. The mechanism involves the use of collagen–alginate tubules and the use of EDTA to increase the porosity of the tubule structures to study their interaction with media and incubation techniques as well as behavior and morphology. Tubules of 1.7% sodium alginate and 0.4% collagen I reacting with 3.2% CaCl2 solution proved to be more stable in both the printing process and …


A State-Of-The-Art Review Of Laser-Assisted Bioprinting And Its Future Research Trends, Chaoran Dou, Victoria Perez, Jie Qu, Andrew Tsin, Ben Xu, Jianzhi Li Jun 2021

A State-Of-The-Art Review Of Laser-Assisted Bioprinting And Its Future Research Trends, Chaoran Dou, Victoria Perez, Jie Qu, Andrew Tsin, Ben Xu, Jianzhi Li

Manufacturing & Industrial Engineering Faculty Publications and Presentations

Bioprinting is an additive manufacturing technology with great potential in medical applications. Among available bioprinting techniques, laser-assisted bioprinting (LAB) is a promising technique due to its high resolution, high cell viability, and the capability to deposit high-viscousity bioink. These characteristics allow the LAB technology to control cells precisely to reconstruct living organs. Recent developments of LAB technologies are reviewed in this paper, covering various designs of LAB printers, research progresses in energy-absorbing layer (EAL), the physical phenomenon that triggers the printing process in terms of bubble formation and jet development, printing process parameters, and major factors related to the post-printing …