Open Access. Powered by Scholars. Published by Universities.®

Manufacturing Commons

Open Access. Powered by Scholars. Published by Universities.®

Selected Works

Discipline
Keyword
Publication Year
Publication
File Type

Articles 1 - 30 of 47

Full-Text Articles in Manufacturing

The Subject Librarian Newsletter, Engineering And Computer Science, Fall 2017, Buenaventura "Ven" Basco Oct 2018

The Subject Librarian Newsletter, Engineering And Computer Science, Fall 2017, Buenaventura "Ven" Basco

Buenaventura "Ven" Basco

No abstract provided.


Advances In Composite Manufacturing Of Helicopter Parts, Tobias A. Weber, Hans-Joachim K. Ruff-Stahl Jul 2018

Advances In Composite Manufacturing Of Helicopter Parts, Tobias A. Weber, Hans-Joachim K. Ruff-Stahl

Hans-Joachim Ruff-Stahl

This study investigates and compares different methods for improving standard autoclave composite manufacturing in order to find suitable approaches to a more efficient composite production. The goal is not only a reduction in manufacturing times and costs but also quality enhancement. Improved part quality while decreasing costs enables a manufacturer of composite parts to expand its market share, especially in the helicopter market, which has been constantly shrinking over the last two years. Various approaches such as improved tooling technology, the use of automated systems for lamination as well as outsourcing are examined to provide an overview of possible advancements …


Three Hundred And Sixty Degree Real-Time Monitoring Of 3-D Printing Using Computer Analysis Of Two Camera Views, Siranee Nuchitprasitchai, Michael C. Roggeman, Joshua M. Pearce Mar 2018

Three Hundred And Sixty Degree Real-Time Monitoring Of 3-D Printing Using Computer Analysis Of Two Camera Views, Siranee Nuchitprasitchai, Michael C. Roggeman, Joshua M. Pearce

Joshua M. Pearce

Prosumer (producing consumer)-based desktop additive manufacturing has been enabled by the recent radical reduction in 3-D printer capital costs created by the open-source release of the self-replicating rapid prototype (RepRap). To continue this success, there have been some efforts to improve reliability, which are either too expensive or lacked automation. A promising method to improve reliability is to use computer vision, although the success rates are still too low for widespread use. To overcome these challenges an open source low-cost reliable real-time optimal monitoring platform for 3-D printing from double cameras is presented here. This error detection system is implemented …


Distributed Manufacturing Of Flexible Products: Technical Feasibility And Economic Viability, Aubrey Woern, Joshua M. Pearce Mar 2018

Distributed Manufacturing Of Flexible Products: Technical Feasibility And Economic Viability, Aubrey Woern, Joshua M. Pearce

Joshua M. Pearce

Distributed manufacturing even at the household level is now well established with the combined use of open source designs and self-replicating rapid prototyper (RepRap) 3-D printers. Previous work has shown substantial economic consumer benefits for producing their own polymer products. Now flexible filaments are available at roughly 3-times the cost of more conventional 3-D printing materials. To provide some insight into the potential for flexible filament to be both technically feasible and economically viable for distributed digital manufacturing at the consumer level this study investigates 20 common flexible household products. The 3-D printed products were quantified by print time, electrical …


Free And Open Source 3-D Model Customizer For Websites To Democratize Design With Openscad, Yuenyong Nilsiam, Joshua M. Pearce Mar 2018

Free And Open Source 3-D Model Customizer For Websites To Democratize Design With Openscad, Yuenyong Nilsiam, Joshua M. Pearce

Joshua M. Pearce

3-D printing has entered the consumer market because of recent radical price declines. Consumers can save substantial money by offsetting purchases with DIY pre-designed 3-D printed products. However, even more value can be obtained with distributed manufacturing using mass customization. Unfortunately, the average consumer is not technically sophisticated enough to easily design their own products. One solution to this is the use of an overlay on OpenSCAD parametric code, although current solutions force users to relinquish all rights to their own designs. There is thus a substantial need in the open source design community for a libre 3-D model customizer, …


Energy Payback Time Of A Solar Photovoltaic Powered Waste Plastic Recyclebot System, Shan Zhong, Pratiksha Rakhe, Joshua M. Pearce Mar 2018

Energy Payback Time Of A Solar Photovoltaic Powered Waste Plastic Recyclebot System, Shan Zhong, Pratiksha Rakhe, Joshua M. Pearce

Joshua M. Pearce

The growth of both plastic consumption and prosumer 3-D printing are driving an interest in producing 3-D printer filaments from waste plastic. This study quantifies the embodied energy of a vertical DC solar photovoltaic (PV) powered recyclebot based on life cycle energy analysis and compares it to horizontal AC recyclebots, conventional recycling, and the production of a virgin 3-D printer filament. The energy payback time (EPBT) is calculated using the embodied energy of the materials making up the recyclebot itself and is found to be about five days for the extrusion of a poly lactic acid (PLA) filament or 2.5 …


Open Source Multi-Head 3d Printer For Polymer-Metal Composite Component Manufacturing, J. Laureto, Joshua M. Pearce Mar 2018

Open Source Multi-Head 3d Printer For Polymer-Metal Composite Component Manufacturing, J. Laureto, Joshua M. Pearce

Joshua M. Pearce

As low-cost desktop 3D printing is now dominated by free and open source self-replicating rapid prototype (RepRap) derivatives, there is an intense interest in extending the scope of potential applications to manufacturing. This study describes a manufacturing technology that enables a constrained set of polymer-metal composite components. This paper provides (1) free and open source hardware and (2) software for printing systems that achieves metal wire embedment into a polymer matrix 3D-printed part via a novel weaving and wrapping method using (3) OpenSCAD and parametric coding for customized g-code commands. Composite parts are evaluated from the technical viability of manufacturing …


Impact Of Diy Home Manufacturing With 3d Printing On The Toy And Game Market, Emily Peterson, Joshua M. Pearce Mar 2018

Impact Of Diy Home Manufacturing With 3d Printing On The Toy And Game Market, Emily Peterson, Joshua M. Pearce

Joshua M. Pearce

The 2020 toy and game market is projected to be US$135 billion. To determine if 3D printing could affect these markets if consumers offset purchases by 3D printing free designs, this study investigates the 100 most popular downloaded designs at MyMiniFactory in a month. Savings are quantified for using a Lulzbot Mini 3D printer and three filament types: commercial filament, pellet-extruded filament, and post-consumer waste converted to filament with a recyclebot. Case studies probed the quality of: (1) six common complex toys; (2) Lego blocks; and (3) the customizability of open source board games. All filaments analyzed saved the user …


Open Source 3-D Printed Nutating Mixer, Dhwani K. Trivedi, Joshua M. Pearce Mar 2018

Open Source 3-D Printed Nutating Mixer, Dhwani K. Trivedi, Joshua M. Pearce

Joshua M. Pearce

As the open source development of additive manufacturing has led to low-cost desktop three-dimensional (3-D) printing, a number of scientists throughout the world have begun to share digital designs of free and open source scientific hardware. Open source scientific hardware enables custom experimentation, laboratory control, rapid upgrading, transparent maintenance, and lower costs in general. To aid in this trend, this study describes the development, design, assembly, and operation of a 3-D printable open source desktop nutating mixer, which provides a fixed 20° platform tilt angle for a gentle three-dimensional (gyrating) agitation of chemical or biological samples (e.g., DNA or blood …


Why Toyota And Honda Topped The 2002 J.D. Power Quality Study, Susan Lightle, Kenneth Yale Rosenzweig, John Talbott Jun 2015

Why Toyota And Honda Topped The 2002 J.D. Power Quality Study, Susan Lightle, Kenneth Yale Rosenzweig, John Talbott

Kenneth Y. Rosenzweig

Toyota again topped the annual J. D. Power and Associates quality study released in late May of 2002. Toyota scored the highest mark ever with l 07 defects per l 00 vehicles, while Honda came in second with 113 defects. The study was based on responses of approximately 65,000 new car owners queried during their first 90-days of ownership. These results do not surprise us, as we have been fortunate to make numerous sojourns to the Toyota plant in Georgetown, Kentucky, and observe the manufacturing processes. These trips were normally facilitated by a former Japanese student of ours, Minako Yanke, …


Methods Of Extrusion On Demand For High Solids Loading Ceramic Paste In Freeform Extrusion Fabrication, Wenbin Li, Amir Ghazanfari, Ming C. Leu, Robert G. Landers Dec 2014

Methods Of Extrusion On Demand For High Solids Loading Ceramic Paste In Freeform Extrusion Fabrication, Wenbin Li, Amir Ghazanfari, Ming C. Leu, Robert G. Landers

Amir Armani (Ghazanfari)

Fabrication of highly dense parts with complex geometry by paste-extrusion-based solid freeform fabrication processes requires a precise control of the extrusion flow rate to dispense material on demand, which is often referred as Extrusion-On-Demand (EOD). The extrusion process for aqueous ceramic pastes is complex and difficult to control due to their non-Newtonian behavior, compressibility and inhomogeneity. In this study, three methods of EOD (based on ram extruder, needle valve, and auger valve) are introduced and investigated for the extrusion of high solids loading (i.e., >50%, volumetric) aqueous alumina paste. Optimal extrusion process parameters for these methods are determined through printing …


Single-Layer Graphene Oxide Reinforced Metal Matrix Composites By Laser Sintering: Microstructure And Mechanical Property Enhancement, Dong Lin Oct 2014

Single-Layer Graphene Oxide Reinforced Metal Matrix Composites By Laser Sintering: Microstructure And Mechanical Property Enhancement, Dong Lin

dong lin

No abstract provided.


Optimization Of Contact Pattern Position On The Worm Wheel Teeth By Axial Shift In Worm Wheel Axis, Ehsan Soury, Arash Houshmand, Alireza Nezamabadi Mar 2014

Optimization Of Contact Pattern Position On The Worm Wheel Teeth By Axial Shift In Worm Wheel Axis, Ehsan Soury, Arash Houshmand, Alireza Nezamabadi

Arash Houshmand

In industrial applications Lubricant temperature decrease is a desired aim in the case of lubricant and gears performance. In this paper the procedure of optimization of worm gears performance by considering the proper contact pattern position had been performed. In accordance with required axial shift in worm wheel axis the contact pattern optimized and as a result the lubricant temperature decreased. This procedure leads to increase gears efficiency and their effective life.


Single-Layer Graphene Oxide Reinforced Metal Matrix Composites By Laser Sintering: Microstructure And Mechanical Property Enhancement, Dong Lin Dec 2013

Single-Layer Graphene Oxide Reinforced Metal Matrix Composites By Laser Sintering: Microstructure And Mechanical Property Enhancement, Dong Lin

dong lin

No abstract provided.


A Case Study Of Heat Treatment On Aisi 1020 Steel, Sayed Shafayat Hossain, Md. Maksudul Islam, Md. Sajibul Alam Bhuyan Dec 2013

A Case Study Of Heat Treatment On Aisi 1020 Steel, Sayed Shafayat Hossain, Md. Maksudul Islam, Md. Sajibul Alam Bhuyan

Md. Maksudul Islam

Proper heat treatment of steels is one of the most important factors in determining how they will perform in service. Engineering materials, mostly steel, are heat treated under controlled sequence of heating and cooling to alter their physical and mechanical properties to meet desired engineering applications. In this study we have chosen AISI 1020 steel as for our research work and we have tried to find out the mechanical properties (hardness) and micro structural properties (martensite formation, carbon self-locking region) by means of appropriate heat treatment process (annealing, normalizing & hardening). Here the steel specimens were heat treated in a …


Inhibition Of Bacillus Cereus Growth By Bacteriocin Producing Bacillus Subtilis Isolated From Fermented Baobab Seeds (Maari) Is Substrate Dependent, Donatien Kaboré, Dennis S. Nielsen, Hagrétoui Sawadogo-Lingan, Bréhima Diawara, Mamoudou H. Dicko Prof., Mogens Jakobsen, Line Thorsen Jan 2013

Inhibition Of Bacillus Cereus Growth By Bacteriocin Producing Bacillus Subtilis Isolated From Fermented Baobab Seeds (Maari) Is Substrate Dependent, Donatien Kaboré, Dennis S. Nielsen, Hagrétoui Sawadogo-Lingan, Bréhima Diawara, Mamoudou H. Dicko Prof., Mogens Jakobsen, Line Thorsen

Pr. Mamoudou H. DICKO, PhD

Maari is a spontaneously alkaline fermented food condiment made from baobab tree seeds. Due to the spontaneous nature of maari fermentations growth of the opportunistic human pathogen Bacillus cereus is occasionally observed. Bacillus subtilis strains are important for alkaline seed fermentations because of their enzymatic activities contributing to desirable texture, flavor and pH development. Some B. subtilis strains have antimicrobial properties against B. cereus. In the present work, three bacteriocin producing B. subtilis strains (B3, B122 and B222) isolated from maari were tested. The production of antimicrobial activity by the three strains was found to be greatly influenced by the …


Comparison Of Phenolic Compounds And Antioxidant Capacities Of Traditional Sorghum Beers With Other Alcoholic Beverages, Fatouma Abdoul-Latif, Romaric G. Bayili, Louis C. Obame, Mamoudou H. Dicko Prof. Oct 2012

Comparison Of Phenolic Compounds And Antioxidant Capacities Of Traditional Sorghum Beers With Other Alcoholic Beverages, Fatouma Abdoul-Latif, Romaric G. Bayili, Louis C. Obame, Mamoudou H. Dicko Prof.

Pr. Mamoudou H. DICKO, PhD

Thirty samples of sorghum beers “dolo” were selected from traditionally fermented household manufacturers from Burkina Faso. Dolo samples were screened for their total phenolic content, proanthocyanidins and putative antioxidant capacities, and were compared with industrial beers and wines. Total phenols were measured using the Folin-Ciocalteu method. Proanthocyanidins content were determined by the method of HCl-butanol hydrolysis. Antioxidant activities were evaluated both with 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and by the trolox equivalent antioxidant capacity (TEAC) using 2,2’-azinobis(3-ethyl-benzothiazoline-6-sulfonic acid radical) (ABTS•+). The average contents of total phenols and proanthocyanidins were 506 μg GAE/ml of dolo and 45 μg APE/ml of dolo, respectively. An …


Characterization Of Conducting-Polymer-Based Bimorph Vibration Sensors, Weihua Li, Geoffrey M. Spinks, Lianbin Zhao, Yanzhe Wu, Dezhi Zhou, G G. Wallace Aug 2012

Characterization Of Conducting-Polymer-Based Bimorph Vibration Sensors, Weihua Li, Geoffrey M. Spinks, Lianbin Zhao, Yanzhe Wu, Dezhi Zhou, G G. Wallace

Professor Weihua Li

This paper presents theoretical and experimental investigation of mechanical-electrical properties of conducting polymers based bimorph sensors. A material parameter, hCP , is proposed to represent linear relationship between induction charge and the applied external deformation. Based on this assumption, a constitutive equation for bimorph sensors under steady-state external loadings are constructed and then solved. Mechanical-electrical properties of bimorph sensors are experimentally studied using both vibration-amplitude sweep mode and frequency sweep mode. The material parameter hCP , is identified by comparing theoretical analysis and experimental results. The applications of conducting polymers based bimorph sensors in smart structures are also discussed.


Toxicity Assessment And Analgesic Activity Investigation Of Aqueous Acetone Extracts Of Sida Acuta Burn F. And Sida Cordifolia L. (Malvaceae), Medicinal Plants Of Burkina Faso, Kiessoum Konate, Adama Hilou, Raïssa Rr Aworet-Samseny, Alain Souza, Nicolas Barro, Mamoudou H. Dicko Prof., Jacques Datté, Bertrand M’Batchi Aug 2012

Toxicity Assessment And Analgesic Activity Investigation Of Aqueous Acetone Extracts Of Sida Acuta Burn F. And Sida Cordifolia L. (Malvaceae), Medicinal Plants Of Burkina Faso, Kiessoum Konate, Adama Hilou, Raïssa Rr Aworet-Samseny, Alain Souza, Nicolas Barro, Mamoudou H. Dicko Prof., Jacques Datté, Bertrand M’Batchi

Pr. Mamoudou H. DICKO, PhD

Background Sida acuta Burn f. and Sida cordifolia L. (Malvaceae) are traditionally used in Burkina Faso to treat several ailments, mainly pains, including abdominal infections and associated diseases. Despite the extensive use of these plants in traditional health care, literature provides little information regarding their toxicity and the pharmacology. This work was therefore designed to investigate the toxicological effects of aqueous acetone extracts of Sida acuta Burn f. and Sida cordifolia L. Furthermore, their analgesic capacity was assessed, in order to assess the efficiency of the traditional use of these two medicinal plants from Burkina Faso. Method For acute toxicity …


Thermoelectric Module-Variable Conductance Heat Pipe Assemblies For Reduced Power Temperature Control, Corey Melnick, Marc Hodes, Gennady Ziskind, Martin Cleary, Vincent Manno Aug 2012

Thermoelectric Module-Variable Conductance Heat Pipe Assemblies For Reduced Power Temperature Control, Corey Melnick, Marc Hodes, Gennady Ziskind, Martin Cleary, Vincent Manno

Vincent P. Manno

Thermoelectric modules (TEMs) are used to precisely maintain the setpoint temperature of photonic components generating variable heat loads under varying ambient conditions. The non-component side of TEMs is mounted onto conventional heat sinks (CHSs). At any combination of setpoint temperature, heat load and ambient temperature, there is a unique thermal resistance between the non-component side of a TEM and the ambient corresponding to minimal TEM power consumption. Indeed, a zero thermal resistance heat sink minimizes power consumption when a TEM operates in refrigeration mode, but when it operates in heating mode a relatively high thermal resistance one is optimal. This …


Investigation Of Thermomechanical Effects Of Lighting Conditions On Canvas Paintings By Laser Shearography, Morteza Khaleghi, Ivo Dobrev, Philip Klausmeyer, Ellery Harrington, Cosme Furlong Jul 2012

Investigation Of Thermomechanical Effects Of Lighting Conditions On Canvas Paintings By Laser Shearography, Morteza Khaleghi, Ivo Dobrev, Philip Klausmeyer, Ellery Harrington, Cosme Furlong

Morteza Khaleghi

Quantitative techniques to characterize thermomechanical effects of light on canvas paintings are necessary in order to better understand the deleterious effects that light has on precious art collections in museum exhibitions. In this paper, we present advances in the development of a customized laser shearography system for temporal characterization of in-plane displacements of canvas paintings when subjected to specific lighting conditions. The shearography system is synchronized with a thermal IR camera and concomitant measurements of derivatives of displacements along two orthogonal shearing directions as well as thermal fields are performed. Due to the nature of the measurements, we have developed …


Improving Endmilling Surface Finish By Workpiece Rotation And Adaptive Toolpath Spacing, Athulan Vijayaraghavan, Aaron Hoover, Jeffrey Hartnett, David Dornfeld Jul 2012

Improving Endmilling Surface Finish By Workpiece Rotation And Adaptive Toolpath Spacing, Athulan Vijayaraghavan, Aaron Hoover, Jeffrey Hartnett, David Dornfeld

Aaron M. Hoover

Free-form surfaces are being used in a growing number of engineering applications, especially in injection molding of consumer products. Decreasing the manufacturing cost and time of these molds will improve the efficiency of manufacturing injection molded consumer products. This paper is motivated by the need for simple strategies to improve the quality of and decrease the time required to machine free-form surfaces. We present two methods to improve the surface finish of parts finished with ball-nose endmilling. In the first method the surface finish is improved by finding an optimal orientation angle for the workpiece relative to the machining axis. …


Trajectory Generation In High-Speed, High-Precision Micromilling Using Subdivision Surfaces, Athulan Vijayaraghavan, Angela Sodemann, Aaron Hoover, J. Mayor, David Dornfeld Jul 2012

Trajectory Generation In High-Speed, High-Precision Micromilling Using Subdivision Surfaces, Athulan Vijayaraghavan, Angela Sodemann, Aaron Hoover, J. Mayor, David Dornfeld

Aaron M. Hoover

Motion control in high-speed micromilling processes requires fast, accurate following of a specified curvilinear path. The accuracy with which the path can be followed is determined by the speed at which individual trajectories can be generated and sent to the control system. The time required to generate the trajectory is dependent on the representations used for the curvilinear trajectory path. In this study, we introduce the use of subdivision curves as a method for generating high-speed micromilling trajectories. Subdivision curves are discretized curves which are specified as a series of recursive refinements of a coarse mesh. By applying these recursive …


Antibacterial Activity Against Beta- Lactamase Producing Methicillin And Ampicillin-Resistants Staphylococcus Aureus: Fractional Inhibitory Concentration Index (Fici) Determination, Kiessoun K Onate, Jacques François Mavoungou, Alexis Nicaise Lepengué, Raïssa Rr Aworet-Samseny, Adama Hilou, Mamoudou H. Dicko Prof., Bertrand M’Batchi Jun 2012

Antibacterial Activity Against Beta- Lactamase Producing Methicillin And Ampicillin-Resistants Staphylococcus Aureus: Fractional Inhibitory Concentration Index (Fici) Determination, Kiessoun K Onate, Jacques François Mavoungou, Alexis Nicaise Lepengué, Raïssa Rr Aworet-Samseny, Adama Hilou, Mamoudou H. Dicko Prof., Bertrand M’Batchi

Pr. Mamoudou H. DICKO, PhD

The present study reports the antibacterial capacity of alkaloid compounds in combination with Methicillin and Ampicillin-resistants bacteria isolated from clinical samples. The resistance of different bacteria strains to the current antibacterial agents, their toxicity and the cost of the treatment have led to the development of natural products against the bacteria resistant infections when applied in combination with conventional antimicrobial drugs. The antibacterial assays in this study were performed by using inhibition zone diameters, MIC, MBC methods, the time-kill assay and the Fractional Inhibitory Concentration Index (FICI) determination. On the whole, fifteen Gram-positive bacterial strains (MRSA/ARSA) were used. Negative control …


Reviving The U.S. Rail And Transit Industry: Investments And Job Creation, Joan Fitzgerald, Lisa Granquist, Ishwar Khatiwada, Joe Mclaughlin, Michael Renner, Andrew Sum Apr 2012

Reviving The U.S. Rail And Transit Industry: Investments And Job Creation, Joan Fitzgerald, Lisa Granquist, Ishwar Khatiwada, Joe Mclaughlin, Michael Renner, Andrew Sum

Joe McLaughlin

No abstract provided.


Reviving The U.S. Rail And Transit Industry: Investments And Job Creation, Joan Fitzgerald, Lisa Granquist, Ishwar Khatiwada, Joe Mclaughlin, Michael Renner, Andrew Sum Apr 2012

Reviving The U.S. Rail And Transit Industry: Investments And Job Creation, Joan Fitzgerald, Lisa Granquist, Ishwar Khatiwada, Joe Mclaughlin, Michael Renner, Andrew Sum

Ishwar Khatiwada

No abstract provided.


Reviving The U.S. Rail And Transit Industry: Investments And Job Creation, Joan Fitzgerald, Lisa Granquist, Ishwar Khatiwada, Joe Mclaughlin, Michael Renner, Andrew Sum Apr 2012

Reviving The U.S. Rail And Transit Industry: Investments And Job Creation, Joan Fitzgerald, Lisa Granquist, Ishwar Khatiwada, Joe Mclaughlin, Michael Renner, Andrew Sum

Andrew Sum

No abstract provided.


Reviving The U.S. Rail And Transit Industry: Investments And Job Creation, Joan Fitzgerald, Lisa Granquist, Ishwar Khatiwada, Joe Mclaughlin, Michael Renner, Andrew Sum Apr 2012

Reviving The U.S. Rail And Transit Industry: Investments And Job Creation, Joan Fitzgerald, Lisa Granquist, Ishwar Khatiwada, Joe Mclaughlin, Michael Renner, Andrew Sum

Joan Fitzgerald

No abstract provided.


Bacteriocin Formation By Dominant Aerobic Sporeformers Isolated From Traditional Maari, Donatien Kabore, Line Thorsen, Dennis Sandris Nielsen, Torben Sune Berner, Hagrétou Sawadogo-Lingani, Bréhima Diawara, Mamoudou H. Dicko Prof., Mogens Jackobsen Jan 2012

Bacteriocin Formation By Dominant Aerobic Sporeformers Isolated From Traditional Maari, Donatien Kabore, Line Thorsen, Dennis Sandris Nielsen, Torben Sune Berner, Hagrétou Sawadogo-Lingani, Bréhima Diawara, Mamoudou H. Dicko Prof., Mogens Jackobsen

Pr. Mamoudou H. DICKO, PhD

The antimicrobial activity of 8 Bacillus spp. and 2 Lysinibacillus spp. representing the predominant aerobic sporeformers during traditional maari fermentations, a traditional fermented baobab seeds product fromBurkina Faso,was investigated. The antimicrobial activitywas assessed against a total of 31 indicator organisms representing various Gram-negative and positive pathogens. The screening showed that 3 Bacillus subtilis strains (B3, B122 and B222) in particular had antimicrobial activity against some Gram-positive organisms and were selected for further studies. Itwas found that the antimicrobial substances producedwere heat stable, in-sensitive to catalase, sensitive to protease and trypsin but resistant to the proteolytic action of papain and proteinase …


Acid Resistance, Bile Tolerance And Antimicrobial Properties Of Dominant Lactic Acid Bacteria Isolated From Traditional “Maari” Baobab Seeds Fermented Condiment, Donatien Kabore, Hagrétou Sawadogo-Lingani, Mamoudou H. Dicko Prof., Bréhima Diawara, Mogens Jacobsen Jan 2012

Acid Resistance, Bile Tolerance And Antimicrobial Properties Of Dominant Lactic Acid Bacteria Isolated From Traditional “Maari” Baobab Seeds Fermented Condiment, Donatien Kabore, Hagrétou Sawadogo-Lingani, Mamoudou H. Dicko Prof., Bréhima Diawara, Mogens Jacobsen

Pr. Mamoudou H. DICKO, PhD

Maari is a fermented food condiment obtained by spontaneous fermentation of seeds from the baobab tree (Adansonia digitata). Nine dominant lactic acid bacteria (LAB) strains, isolated from traditional maari fermentation were examined for their resistance to pH 2.5, their tolerance to 0.3% bile and their antimicrobial activities against pathogenic bacteria. The agar spot test was used to screen the dominant LAB for antagonistic activity against a total of 21 indicator organisms including Bacillus cereus strains, Salmonella spp., Listeria monocytogenes, Yersinia enterocolitica, Escherichia coli and Micrococcus luteus. It was observed that all LAB strains survived in 0.3% bile and exhibited antimicrobial …