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Biomedical Commons™

Open Access. Powered by Scholars. Published by Universities.®

Old Dominion University

2019

Articles 1 - 5 of 5

Full-Text Articles in Biomedical

Effects Of Pulse Width On He Plasma Jets In Contact With Water Evaluated By Oh(A-X) Emission And Ohaq Production, Shutong Song, Esin B. Sözer, Chunqi Jiang Jun 2019

Effects Of Pulse Width On He Plasma Jets In Contact With Water Evaluated By Oh(A-X) Emission And Ohaq Production, Shutong Song, Esin B. Sözer, Chunqi Jiang

Bioelectrics Publications

Nanosecond pulsed helium plasma jets impinging on water produce hydroxyl radicals both in gas- and liquid-phase. In this study, the effects of pulse width on a repetitively pulsed plasma jet in contact with water are evaluated via OH(A-X) emission and OHaq production in water for various pulse widths ranging from 200 to 5000 ns. The maximal energy efficiency of OH(A-X) emission is obtained for pulse widths of 600-800 ns whereas the maximal efficiency of OHaq production is at 200 ns. Temporally-resolved emission spectroscopy shows that more than 40% of OH(A-X) emission is produced during the first 200 ns …

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Mechanisms And Immunogenicity Of Nspef-Induced Cell Death In B16f10 Melanoma Tumors, Alessandra Rossi, Olga N. Pakhomova, Andrei G. Pakhomov, Samantha Weygandt, Anna A. Bulysheva, Len E. Murray, Peter A. Mollica, Claudia Muratori Jan 2019

Mechanisms And Immunogenicity Of Nspef-Induced Cell Death In B16f10 Melanoma Tumors, Alessandra Rossi, Olga N. Pakhomova, Andrei G. Pakhomov, Samantha Weygandt, Anna A. Bulysheva, Len E. Murray, Peter A. Mollica, Claudia Muratori

Bioelectrics Publications

Accumulating data indicates that some cancer treatments can restore anticancer immunosurveillance through the induction of tumor immunogenic cell death (ICD). Nanosecond pulsed electric fields (nsPEF) have been shown to efficiently ablate melanoma tumors. In this study we investigated the mechanisms and immunogenicity of nsPEF-induced cell death in B16F10 melanoma tumors. Our data show that in vitro nsPEF (20-200, 200-ns pulses, 7 kV/cm, 2 Hz) caused a rapid dose-dependent cell death which was not accompanied by caspase activation or PARP cleavage. The lack of nsPEF-induced apoptosis was confirmed in vivo in B16F10 tumors. NsPEF also failed to trigger ICD-linked responses such …

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Nanosecond Pulsed Electric Fields Induce Endoplasmic Reticulum Stress Accompanied By Immunogenic Cell Death In Murine Models Of Lymphoma And Colorectal Cancer, Alessandra Rossi, Olga N. Pakhomova, Peter A. Mollica, Maura Casciola, Uma Mangalanathan, Andrei G. Pakhomov, Claudia Muratori Jan 2019

Nanosecond Pulsed Electric Fields Induce Endoplasmic Reticulum Stress Accompanied By Immunogenic Cell Death In Murine Models Of Lymphoma And Colorectal Cancer, Alessandra Rossi, Olga N. Pakhomova, Peter A. Mollica, Maura Casciola, Uma Mangalanathan, Andrei G. Pakhomov, Claudia Muratori

Bioelectrics Publications

Depending on the initiating stimulus, cancer cell death can be immunogenic or non-immunogenic. Inducers of immunogenic cell death (ICD) rely on endoplasmic reticulum (ER) stress for the trafficking of danger signals such as calreticulin (CRT) and ATP. We found that nanosecond pulsed electric fields (nsPEF), an emerging new modality for tumor ablation, cause the activation of the ER-resident stress sensor PERK in both CT-26 colon carcinoma and EL-4 lymphoma cells. PERK activation correlates with sustained CRT exposure on the cell plasma membrane and apoptosis induction in both nsPEF-treated cell lines. Our results show that, in CT-26 cells, the activity of …

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Quantifying The Concentration And Penetration Depth Of Long-Lived Rons In Plasma Activated Water By Uv Absorption Spectroscopy, Zhijie Liu, Chunxi Zhou, Dingxin Liu, Tongtong He, Li Guo, Dehui Xu, Michael G. Kong Jan 2019

Quantifying The Concentration And Penetration Depth Of Long-Lived Rons In Plasma Activated Water By Uv Absorption Spectroscopy, Zhijie Liu, Chunxi Zhou, Dingxin Liu, Tongtong He, Li Guo, Dehui Xu, Michael G. Kong

Bioelectrics Publications

Reactive oxygen and reactive nitrogen species (RONS) are believed to play a key role in biomedical applications, which means that RONS must reach the target tissue to produce a therapeutic effect. Existing methods (electron spin spectrometry and microplate reading) to determine the RONS concentration are not suitable for experimental real-time measurements because they require adding an indicating reagent to the plasma-treated medium, which may alter the chemical composition of the medium. In this paper, we propose a method to measure the long-lived RONS concentration in plasma-activated water (PAW) by using UV absorption spectroscopy. Based on an analysis and fit of …

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Microstructural Evaluation Of Aluminium Alloy A365 T6 In Machining Operation, Bankole I. Oladapo, S. Abolfazl Zahedi, Francis T. Omigbodun, Edwin A. Oshin, Victor A. Adebiyi, Olaoluwa B. Malachi Jan 2019

Microstructural Evaluation Of Aluminium Alloy A365 T6 In Machining Operation, Bankole I. Oladapo, S. Abolfazl Zahedi, Francis T. Omigbodun, Edwin A. Oshin, Victor A. Adebiyi, Olaoluwa B. Malachi

Electrical & Computer Engineering Faculty Publications

The optimum cutting parameters such as cutting depth, feed rate, cutting speed and magnitude of the cutting force for A356 T6 was determined concerning the microstructural detail of the material. Novel test analyses were carried out, which include mechanical evaluation of the materials for density, glass transition temperature, tensile and compression stress, frequency analysis and optimisation as well as the functional analytic behaviour of the samples. The further analytical structure of the particle was performed, evaluating the surface luminance structure and the profile structure. The cross-sectional filter profile of the sample was extracted, and analyses of Firestone curve for the …

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