Environmental Engineering Commons^™

Parallel Synthesis Of Poly(Amino Ether)-Templated Plasmonic Nanoparticles For Transgene Delivery, James Ramos, Thrimoorthy Potta, Olivia Scheideler, Kaushal Rege

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Plasmonic nanoparticles have been increasingly investigated for numerous applications in medicine, sensing, and catalysis. In particular, gold nanoparticles have been investigated for separations, sensing, drug/nucleic acid delivery, and bioimaging. In addition, silver nanoparticles demonstrate antibacterial activity, resulting in potential application in treatments against microbial infections, burns, diabetic skin ulcers, and medical devices. Here, we describe the facile, parallel synthesis of both gold and silver nanoparticles using a small set of poly(amino ethers), or PAEs, derived from linear polyamines, under ambient conditions and in absence of additional reagents. The kinetics of nanoparticle formation were dependent on PAE concentration and chemical composition. …

Equity And Fairness In Transport Planning: The State Of Play, Sarah Rock, Aoife Ahern Prof., Brian Caulfield Dr.

Conference Papers

This paper explores the concept of equity, or fairness, in transport. As a pillar of sustainable development, social equity is an important objective of transport planning. The provision of transport infrastructure can have significant equity impacts on society through the distribution of costs and benefits. In recent years, there has been an increase in research interest in transportation related equity issues. The paper outlines the primary theoretical traditions that relate to equity and transport equity, and how equity concerns are currently addressed and evaluated in academia and in practice. Recent research has attempted to establish stronger principles from which to …

Seed Tape Effects On Corn Emergence Under Greenhouse Conditions, Ramarao Venkatesh, Peter R. Thomison, Colette K. Gabriel, Mark A. Bennett, Elaine M. Grassbaugh, Matthew D. Kleinhenz, Scott A. Shearer, Santosh Pitla

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Seed tape has recently received attention as an alternative planting system for smallholder farmers in underdeveloped regions of South America, Africa, China, and India (Mateus, 2014). Seed companies are also developing seed-tape planting systems for germplasm evaluations (Deppermann et al., 2013). Although seed tape has been promoted as a method for ensuring uniform seed spacing and plant density of small-seeded flowers, herbs, and vegetables (Chancellor, 1969), little or no information is available on the use of seed tape for larger-seeded row crops and its effect on crop emergence. The objective of this study was to compare the emergence of corn …

Nutrient Concentrations Of Runoff As Affected By The Diameter Of Unconsolidated Material From Feedlot Surfaces, John E. Gilley, Gregory Boone, David B. Marx

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Beef cattle feedlots contain unconsolidated material that accumulates on the feedlot surface during a feeding cycle. This study was conducted to measure the effects of varying diameters of unconsolidated surface material and varying flow rates on nutrient concentrations in runoff. Unconsolidated surface material with an average diameter of 4.76, 9.53, 19.1, or 47.5 mm and a composite sample with a 15.2 mm mean diameter were placed within 0.75 m wide × 4.0 m long plot areas. Flow was then introduced at the top of the plots in successive increments, and runoff samples for water quality analyses were obtained. Particle diameter …

Soybean Yield, Evapotranspiration, Water Productivity, And Soil Water Extraction Response To Subsurface Drip Irrigation And Fertigation, Suat Irmak, James E. Specht, Lameck O. Odhiambo, J. M. Rees, K. G. Cassman

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Soybean [Glycine max (L.) Merr.] yield, irrigation water use efficiency (IWUE), crop water use efficiency (CWUE), evapotranspiration water use efficiency (ETWUE), and soil water extraction response to eleven treatments of full, limited, or delayed irrigation versus a rainfed control were investigated using a subsurface drip irrigation (SDI) system at a research site in south-central Nebraska. The SDI system laterals were 0.40 m deep in every other row middle of 0.76 m spaced plant rows. Actual evapotranspiration (ET_a) was quantified in all treatments and used to schedule irrigation events on a 100% ET_a replacement basis in all but …

Combined Qcm-D/Ge As A Tool To Characterize Stimuli-Responsive Swelling Of And Protein Adsorption On Polymer Brushes Grafted Onto 3d-Nanostructures, Meike Koenig, Tadas Kasputis, Daniel Schmidt, Keith B. Rodenhausen Jr., Klaus-Jochen Eichhorn, Angela K. Pannier, Mathias Schubert, Manfred Stamm, Petra Uhlmann

Department of Agricultural and Biological Systems Engineering: Faculty Publications

A combined setup of quartz crystal microbalance and generalized ellipsometry can be used to comprehensively investigate complex functional coatings comprising stimuli-responsive polymer brushes and 3D nanostructures in a dynamic, noninvasive in situ measurement. While the quartz crystal microbalance detects the overall change in areal mass, for instance, during a swelling or adsorption process, the generalized ellipsometry data can be evaluated in terms of a layered model to distinguish between processes occurring within the intercolumnar space or on top of the anisotropic nanocolumns. Silicon films with anisotropic nanocolumnar morphology were prepared by the glancing angle deposition technique and further functionalized by …

Water-Stable Three-Dimensional Ultrafine Fibrous Scaffolds From Keratin For Cartilage Tissue Engineering, Helan Xu, Shaobo Cai, Lan Xu, Yiqi Yang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Intrinsically water-stable scaffolds composed of ultrafine keratin fibers oriented randomly and evenly in three dimensions were electrospun for cartilage tissue engineering. Keratin has been recognized as a biomaterial that could substantially support the growth and development of multiple cell lines. Besides, three-dimensional (3D) ultrafine fibrous structures were preferred in tissue engineering due to their structural similarity to native extracellular matrices in soft tissues. Recently, we have developed a nontraditional approach to developing 3D fibrous scaffolds from alcohol-soluble corn protein, zein, and verified their structural advantages in tissue engineering. However, keratin with highly cross-linked molecular structures could not be readily dissolved …

The Hydraulic Conductivity Structure Of Gravel-Dominated Vadose Zones Within Alluvial Floodplains, Ronald B. Miller, Derek M. Heeren, Garey A. Fox, Todd Halihan, Daniel E. Storm, Aaron R. Mittelstet

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The floodplains of many gravel-bed streams have a general stratigraphy that consists of a layer of topsoil covering gravel-dominated subsoil. Previous research has demonstrated that this stratigraphy can facilitate preferential groundwater flow through focused linear features, such as paleochannels, or gravelly regions within the vadose zone. These areas within the floodplain vadose zone may provide a route for interactions between the floodplain surface and alluvial groundwater, effectively extending the hyporheic zone across the floodplain during high stream stage. The objective of this research was to assess the structure and scale of texture heterogeneity within the vadose zone within the gravel …

Simulation Supported Estimation Of End-To-End Transmission Parameters In Non-Viral Gene Delivery, Beata J. Wysocki, Tadeusz A. Wysocki, Timothy M. Martin, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Communications, in general, involve delivery of information from a source to a sink. At nano-scale, an example of a man-made communications involving interfacing with biological systems at intra-cellular level is non-viral gene delivery. From a telecommunications engineering perspective, important end-to-end parameters of such a system are: the endto- end delay, system capacity, and packet loss rate. There are neither known methods to estimate those parameters theoretically nor they are ready available from standard measurements. The paper provides estimates for those parameters based on the simulation of non-viral gene delivery system based on the queuing theory. The simulator used has been …

Dynamic Analysis Of Dna Nanoparticle Immobilization To Model Biomaterial Substrates Using Combinatorial Spectroscopic Ellipsometry And Quartz Crystal Microbalance With Dissipation, Tadas Kasputis, Alex Pieper, Mathias Schubert, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Gene expression within cells can be altered through gene delivery approaches, which have tremendous potential for gene therapy, tissue engineering, and diagnostics. Substrate-mediated gene delivery describes the delivery of plasmid DNA or DNA complexed with nonviral vectors to cells from a surface, with the DNA immobilized to a substrate through specific or nonspecific interactions. In this work, DNA-nanoparticle (DNA–NP) adsorption to substrates is evaluated using combinatorial, in situ spectroscopic ellipsometry and quartz crystal microbalance with dissipation (SE/QCM-D), to evaluate the basic dynamic processes involved in the adsorption and immobilization of DNA–NP complexes to substrates. The concentration of DNA–NP solutions influences …

Implementing Green Roofs On Movie Theaters And Shopping Centers: Business Cases In Profitable Sustainability, Ryan J. Miller

Pomona Senior Theses

This thesis presents the business case for installing green roofs on movie theaters and shopping centers. These businesses can then derive increased profits from the environmental benefits of reduced energy use and increased stormwater retention. After presenting the basic design and benefits of a green roof, the thesis develops stand-alone business plans for a movie theater and shopping center. The author finds that green roofs are a profitable sustainability solution for the commercial enterprise.

Long-Term Activity Of Biohybrid Coatings Of Atrazine-Degrading Bacteria Pseudomonas Sp. Adp, Angela K. Pannier, T. Lehrer, M. Vogel, U. Soltmann, H. Bottcher, S. Tarre, M. Green, J. Raff, K. Pollmann

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The atrazine-degrading bacterial strain Pseudomonas sp. ADP was immobilized by the sol–gel process within thin silica layers coated onto water-retaining carrier materials (expanded clay pellets and scoria). The performance of the obtained biohybrid material has been investigated concerning long-term activity under non-growth conditions. Experiments were run in phosphate buffer containing atrazine (20 mg l^-1) as the sole source of carbon and nitrogen. Even after one year of consecutive batch tests, P. ADP immobilized onto expanded clay pellets showed a high atrazine degradation activity. In the course of long-term batch experiments, the average amount of removed atrazine was about …

Use Of Controller Area Network (Can) Data To Determine Field Efficiencies Of Agricultural Machinery, Santosh Pitla, N. Lin, Joe D. Luck, Scott A. Shearer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Controller Area Network (CAN) bus is used on current agricultural equipment for in-vehicle communication among multiple microcontrollers. In this study, CAN data was collected from the tractor diagnostic port during three field operations: anhydrous ammonia (NH₃) application, field cultivation, and planting. Specifically, CAN messages corresponding to engine fuel use rate were filtered, decoded, and analyzed. High fuel use rates were recorded from the tractor when implements were engaged in the soil compared to when the implements were lifted out of the ground at the end of the passes or, when the tractor was turning and idling. Threshold fuel …

Surface-Enhanced Raman Spectral Biomarkers Correlate With Ankle Brachial Index And Characterize Leg Muscle Biochemical Composition Of Patients With Peripheral Arterial Disease, Kim Cluff, Abby M. Kelly, Panagiotis Koutakis, Xiang N. He, Xi Huang, Yongfeng Lu, Iraklis I. Pipinos, George P. Casale, Jeyamkondan Subbiah

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Peripheral arterial disease (PAD) is characterized by atherosclerotic blockages of the arteries supplying the lower extremities, which cause a progressive accumulation of ischemic injury to the skeletal muscles of the lower limbs. This injury includes altered metabolic processes, damaged organelles, and compromised bioenergetics in the affected muscles. The objective of this study was to explore the association of Raman spectral signatures of muscle biochemistry with the severity of atherosclerosis in the legs as determined by the Ankle Brachial Index (ABI) and clinical presentation. We collected muscle biopsies from the gastrocnemius (calf muscle) of five patients with clinically diagnosed claudication, five …

Intrinsically Water-Stable Keratin Nanoparticles And Their In Vivo Biodistribution For Targeted Delivery, Helan Xu, Zhen Shi, Narendra Reddy, Yiqi Yang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Highly water-stable nanoparticles of around 70 nm and capable of distributing with high uptake in certain organs of mice were developed from feather keratin. Nanoparticles could provide novel veterinary diagnostics and therapeutics to boost efficiency in identification and treatment of livestock diseases to improve protein supply and ensure safety and quality of food. Nanoparticles could penetrate easily into cells and small capillaries, surpass detection of the immune system, and reach targeted organs because of their nanoscale sizes. Proteins with positive and negative charges and hydrophobic domains enable loading of various types of drugs and, hence, are advantageous over synthetic polymers …

Elementary Implantable Force Sensor For Smart Orthopaedic Implants, Rebecca A. Wachs, David Ellstein, John Drazan, Colleen P. Healey, Richard L. Uhl, Kenneth A. Connor, Eric H. Ledet

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Implementing implantable sensors which are robust enough to maintain long term functionality inside the body remains a significant challenge. The ideal implantable sensing system is one which is simple and robust; free from batteries, telemetry, and complex electronics. We have developed an elementary implantable sensor for orthopaedic smart implants. The sensor requires no telemetry and no batteries to communicate wirelessly. It has no on-board signal conditioning electronics. The sensor itself has no electrical connections and thus does not require a hermetic package. The sensor is an elementary L-C resonator which can function as a simple force transducer by using a …

Effects Of Waste Placement Practices On The Engineering Response Of Municipal Solid Waste, Jason Taylor Cox

Master's Theses

An extensive laboratory and field investigation was conducted at Santa Maria Regional Landfill (SMRL) in Santa Maria, CA to determine the effects of waste placement practices on the engineering response of municipal solid waste (MSW). Laboratory and field testing was used to determine the engineering properties and monitor field response of MSW.

The specific gravity (G_s) of manufactured MSW (MMSW), fresh MSW (FMSW), and old MSW (OMSW) was determined experimentally using a modified version of standard soil testing procedures. Effects of particle size, compactive effort, and degradation on the specific gravity of waste were evaluated. Specific gravity …

Compression Testing And Failure Modes Of Steel-Concrete Composite (Sc) Structures For Nuclear Containment, Patrick Michael Wanamaker, Amit H. Varma

The Summer Undergraduate Research Fellowship (SURF) Symposium

Although being able to provide much cleaner power than burning coal and other fossil fuels, nuclear power plants are still a tough sell to the general public due to their history of being spontaneously dangerous. The containment structures surrounding these nuclear plants, however, can play a huge role in reducing the risks associated with them. Relatively new designs for these containment assemblies, known as SC (steel-concrete composite) structures, aim to increase the strength and durability of the containment facilities while keeping costs down. By varying the spacing between shear studs, the ratio of concrete to steel, and the ratio of …

Passive Stormwater Samplers For Sampling Highway Runoff From Bmps: Feasibility Studies, Mitchell R. Klein

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Pollution from highway stormwater runoff has been an increasing area of concern within the environmental field. To respond to the need for reduced contamination within runoff many Best Management Practices (BMPs) have been implemented. One difficult aspect of BMPs is monitoring their effectiveness along with determining effluent concentrations. The current methods for stormwater sampling include sending technicians or installing an auto-sampler to collect either grab or composite samples. These methods become costly, cumbersome and infeasible due to the potentially large amount of BMPs across a region and the irregularity and difficulty of predicting storms. Passive samplers have proven themselves as …

Laboratory Evaluation Of A Turn Compensation Control System For A Ground Sprayer, Wesley M. Porter, Jorge A. Rascon, Yeyin Shi, Randal K. Taylor, Paul A. Weckler

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The ability to compensate sprayer nozzle flow across a horizontal boom has the potential to mitigate the problem of inaccurate chemical application rate due to lateral speed differences when sprayers are turning. A laboratory testing platform and procedure were developed and tested with a commercial turn compensation control system for a ground sprayer. Virtual paths consisting of simulated GPS signals representing right and left turns separated by straight segments were simulated with four turning radii (75%, 125%, 250%, and 500%) based on boom width. Actual application rates from 11 nozzles spread across the boom were measured using load cells in …

Transient And Quasi-Steady Thermal Behaviour Of A Building Envelope Due To Retrofitted Cavity Wall And Ceiling Insulation, Aimee Byrne, Gerard Byrne, Anna Davies, Anthony Robinson

Articles

Accurate understanding of the thermal behaviour of building components is essential for predicting heat-ing or cooling needs and facilitates the implementation of more successful energy saving strategies and retrofits. This paper focuses on a specific measure commonly introduced through the residential energy efficiency retrofit programmes in Ireland–insulation. Traditionally, assessments of the performance of building envelopes have been based on assumed thermal resistances of the materials involved, labora-tory tests and computer modelling. The aim of the present work is to investigate the in situ thermal behaviour of a case study building and its components under transient and quasi-steady environmental conditions, comparing …

Evapotranspiration And Surface Energy Balance Of A Common Reed-Dominated Riparian System In The Platte River Basin, Central Nebraska, Isa Kabenge, Suat Irmak, John E. Gilley, George E. Meyer, Stevan Z. Knezevic, Timothy J. Arkebauer, Duane Woodward, Milton Moravek

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Quantifying actual evapotranspiration (ET_a) of riparian zones is important for more robust water balance analyses that will enable better planning, managing, and allocating of water resources as well as developing strategies to protect delicate riparian ecosystem functions. The ET_a, sensible heat flux (H), net radiation (R_n), soil heat flux (G), meteorological variables (air temperature, T_a; incoming shortwave radiation, R_s; wind speed, u₃; relative humidity, RH; vapor pressure deficit, VPD; precipitation, etc.), and albedo were measured on an hourly time step, and leaf area index (LAI) and plant height …

Acetyl-Coa Carboxylases Are Checkpoints In Adipocyte Differentiation, Elizabeth Lyn Cordonier, Daniel Camara Teixeira, Zhonghi Han, Angela K. Pannier, Janos Zempleni

Department of Agricultural and Biological Systems Engineering: Faculty Publications

No abstract provided.

Variability Of Extreme Precipitation In Coastal River Basins Of The Southern Mexican Pacific Region, Nancy Perez-Morga, Thomas Kretzschmar, Tereza Cavazos, Stephen V. Smith, Francisco Munoz-Arriola

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Extreme wet and dry years (± 1 standard deviation, respectively), as well as the top 95 percentile (P95) of daily precipitation events, derived from tropical cyclone (TC) and nontropical cyclone (NTC) rainfall, were analyzed in coastal river basins in Southern Oaxaca, Mexico (Río Verde, Río Tehuantepec, and the Southern Coast). The study is based on daily precipitation records from 47 quality-controlled stations for the 1961 to 1990 period and TC data for the Eastern Tropical Pacific (EPAC). The aim of this study was to evaluate extreme (dry and wet) trends in the annual contribution of daily P95 precipitation events and …

Hourly And Daily Single And Basal Evapotranspiration Crop Coefficients As A Function Of Growing Degree Days, Days After Emergence, Leaf Area Index, Fractional Green Canopy Cover, And Plant Phenology For Soybean, Suat Irmak, Lameck O. Odhiambo, James Specht, Koffi Djaman

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Hourly evapotranspiration (ET) crop coefficients (K_c) are needed to optimize the effectiveness and efficiency of high-frequency micro- and sprinkler irrigation practices involving the application of water multiple times a day. However, not much is known about the daily and seasonal patterns and magnitudes in hourly K_c values for soybean. In addition, locally developed K_c values are necessary for more robust within-season irrigation management, crop ET estimation, and water balance analyses. Hourly and daily Kc functions were developed for soybean in south-central Nebraska through extensive field research. Actual crop evapotranspiration (ET_a) was measured using a …

Transport Of Steroid Hormones In The Vadose Zone After Land Application Of Beef Cattle Manure, Simon Van Donk, Sagor Biswas, William L. Kranz, Daniel D. Snow, Shannon L. Bartelt-Hunt, Terry L. Mader, Charles A. Shapiro, David P. Shelton, David D. Tarkalson, Tian .. Zhang, Steve Ensley

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Few studies have been conducted to investigate the potential contamination of groundwater from manureborne steroid hormones. The objective of this study was to monitor leaching of steroid hormones at the bottom of 2.4 m deep percolation lysimeters installed in plots treated with beef cattle manure. Soil samples were also collected from surrounding field plots. Treatments consisted of application of manure from two treatment methods (stockpiling and composting) or no manure application. Laboratory analyses of manure, soil, and leachate samples used liquid chromatography and tandem mass spectrometry to identify 17 steroid hormones and metabolites. Progesterone, estrone, β-zearalenol, and 4-androstenedione were detected …

Network Analysis Of Endogenous Gene Expression Profiles After Polyethyleneimine-Mediated Dna Delivery, Timothy M. Martin, Sarah A. Plautz, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background—DNA delivery systems, which transport exogenous DNA to cells, have applications that include gene therapy, tissue engineering and medical devices. Although the cationic nonviral DNA carrier polyethyleneimine (PEI) has been widely studied, the molecular factors and pathways underlying PEI-mediated DNA transfer remain largely unknown, preventing the design of more efficient delivery systems.

Methods—HEK 293 T cells were treated with polyplexes formed with PEI and pEGFPLuc encoding for green fluorescent protein (GFP). Transfected cells expressing GFP were flowseparated from treated, untransfected cells. Gene expression profiles were obtained using Affymetrix HG-U133 2.0 microarrays and differentially expressed genes were identified using R/ Bioconductor. …

Avhrr-Ndvi-Based Crop Coefficients For Analyzing Long-Term Trends In Evapotranspiration In Relation To Changing Climate In The U.S. High Plains, Denis Mutiibwa, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Studies in regions of extensive irrigation practices have revealed a significant influence of evaporative cooling on regional temperatures as a result of surface energy redistribution during evaporation. In the U.S. High Plains, maximum temperatures during the last quarter of the 20th century have been decreasing. We investigated the trends in evapotranspiration (ET or latent heat) fluxes originating from increasing irrigation practices in the High Plains region from 1981 to 2008. We estimated actual ET (ETc) over the entire High Plains from the spatial crop coefficients (Kc) and spatial reference (potential) ET (ETref). We proposed …

Runoff Nutrient Transport As Affected By Land Application Method, Swine Growth Stage, And Runoff Rate, J. E. Gilley, Shannon L. Bartelt-Hunt, S. J. Lamb, X. Li, D. B. Marx, D. D. Snow, D. B. Parker, B. L. Woodbury

Department of Agricultural and Biological Systems Engineering: Faculty Publications

This study was conducted to measure the effects of slurry application method, swine growth stage, and flow rate on runoff nutrient transport. Swine slurry was obtained from production units containing grower pigs, finisher pigs, or sows and gilts. The swine slurry was applied using broadcast, disk, or injection methods at a rate required to meet annual nitrogen requirements for corn. Three 30 min simulated rainfall events, separated by 24 h intervals, were applied to the experimental plots at an intensity of 70 mm h^-1. Inflow was applied at the top of each plot in four successive increments after …

Maize Evapotranspiration, Yield Production Functions, Biomass, Grain Yield, Harvest Index, And Yield Response Factors Under Full And Limited Irrigation, Koffi Djaman, Suat Irmak, William R. Rathje, Derrel L. Martin, Dean E. Eisenhauer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

South-central Nebraska is one of the most extensively irrigated areas in the U.S., with over 65,000 active irrigation wells, and maize is the major agronomical crop produced. Maize production in this region requires supplementary irrigation for maximum productivity. Effective on-farm implementation of full and limited irrigation practices for potential improvements of crop productivity requires knowledge of locally developed crop yield response to water functions. In this study, the effects of full and limited irrigation practices on maize (Zea mays L.) plant height, leaf area index (LAI), grain yield and biomass production, actual crop evapotranspiration (ET_a), yield production functions, …