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Articles 1 - 30 of 12711
Full-Text Articles in Civil and Environmental Engineering
Dynamic Baseline Schedule Updates Using Digital Twinning: A Case Study On Aluminum Formwork Construction, Ahmed Hamdi Sweilam
Dynamic Baseline Schedule Updates Using Digital Twinning: A Case Study On Aluminum Formwork Construction, Ahmed Hamdi Sweilam
Theses and Dissertations
Repetitive high-rise construction using aluminum formwork couples consecutive floor cycles through a reused panel set and a floor-level inspection and pour barrier, so productivity loss compounds upward rather than being absorbed locally. The baseline schedule is nevertheless fixed before execution and is not revised as evidence of realized productivity accumulates. This thesis develops a simulation-based digital twin that updates the baseline from observed as-built durations and acts on its own forecast. A discrete-event simulation of a ten-story residential tower is built in AnyLogic® and verified analytically at 180.00 hours per floor. After each completed floor the twin infers activity productivity …
Simulation-Based Assessment Of Operational And Retrofit Solutions For Effluent Compliance Of A Capacity-Limited Oxidation Ditch Wwtp, Ahmed Sameh Harash
Simulation-Based Assessment Of Operational And Retrofit Solutions For Effluent Compliance Of A Capacity-Limited Oxidation Ditch Wwtp, Ahmed Sameh Harash
Theses and Dissertations
Oxidation-ditch wastewater treatment plants (WWTPs) across Egypt's Nile Delta are increasingly carrying hydraulic and organic loads beyond what they were designed for. This work can be considered pioneering as this work compared operational and structural retrofit alternatives for such a plant within a single verified simulation framework. The present study fills that gap by building a digital twin of the Qaha WWTP, a dual-train Carrousel oxidation ditch in Qalyubia Governorate, calibrated to a baseline flow of 7,420 m³/day in the SUMO24 platform (a third-generation, open-source wastewater treatment process simulation software) using the SUMO2c biokinetic model (a simplified variant of Activated …
Sustainable – Low-Cost - Upgrade Of Activated Sludge Process For Nitrogen And Phosphorus (N&P) Removal From Wastewater, Abanoub N. Basta
Sustainable – Low-Cost - Upgrade Of Activated Sludge Process For Nitrogen And Phosphorus (N&P) Removal From Wastewater, Abanoub N. Basta
Theses and Dissertations
Many municipal wastewater treatment plants (WWTPs) in developing countries were designed primarily for organic matter and suspended solids removal and therefore face increasing difficulty in meeting modern nitrogen (N) and phosphorus (P) discharge requirements. Conventional biological nutrient removal upgrades commonly require additional reactor volume, recirculation systems, aeration energy, and capital investment. Accordingly, sustainable retrofit strategies that improve nutrient removal within existing treatment footprints are increasingly needed, particularly in water-scarce and land-constrained regions.
This study investigated the integration of water hyacinth (Eichhornia crassipes) into the aeration stage of an activated sludge process as a hybrid nature-based enhancement for nitrogen and phosphorus …
A Comparative Study: Force Majeure And The Theory Of Unforeseen Circumstances Across Different Jurisdictions In The Middle East, Omar Hamed Sawan
A Comparative Study: Force Majeure And The Theory Of Unforeseen Circumstances Across Different Jurisdictions In The Middle East, Omar Hamed Sawan
Theses and Dissertations
The motivation behind this study stems from recent global disruption events that impact construction projects worldwide. These events vary across a wide spectrum, whether they are political events such as the Russo-Ukraine war or the war in the Middle East, economic events such as inflation or currency devaluations; or environmental events such as climate change and the emergence of public health crises like the Covid-19 pandemic. These series of events underscore the growing importance of understanding how the different legal frameworks address unforeseeable events in the construction industry. In this context, force majeure and the theory of unforeseen circumstances (also …
Enhancing Agricultural Sustainability Under Climate Change: A Multi-Scale Framework Integrating Climate Extremes, Resource Efficiency, And Data-Driven Modeling, Shahryar Fazli
Computational and Data Sciences (PhD) Dissertations
Agricultural systems are increasingly challenged by climate variability, where shifting temperature regimes, hydrological variability, and the rising frequency of compound and cascading extremes threaten global food security and resource sustainability. Addressing these challenges requires integrated frameworks that bridge biophysical monitoring, predictive modeling, and adaptive decision-making. This dissertation develops a data-driven, multi-scale framework to quantify and enhance agricultural resilience by integrating remote sensing, climate analytics, and machine learning across the United States, with a focus on California and the Western U.S.
First, hyperspectral and thermal remote sensing data from EMIT and OpenET are integrated to characterize crop nitrogen–water interactions and assess …
Scalable Quality Assessment Of Ground Motion Records Via Interpretable Deep Learning Architectures, Ali Montazeri Namin
Scalable Quality Assessment Of Ground Motion Records Via Interpretable Deep Learning Architectures, Ali Montazeri Namin
All Graduate Theses and Dissertations, Fall 2023 to Present
Earthquake engineers rely on accurate recordings of ground shaking to design safe and resilient buildings. However, sorting through thousands of these recordings to find the reliable ones and throwing out those ruined by sensor errors or background noise is traditionally done by hand. Because modern seismic networks collect massive amounts of earthquake data every day, this manual checking process is much too slow. To fix this, researchers are turning to artificial intelligence to automatically check the quality of these recordings.
While artificial intelligence offers a fast solution, there are limitations. These computer models can become massive and expensive to run, …
Identifying Influential Drivers To The Utilization Of Prefabrication On Student Housing At Cal Poly: A Case Study On The Delivery Method, Dominique Giang
Identifying Influential Drivers To The Utilization Of Prefabrication On Student Housing At Cal Poly: A Case Study On The Delivery Method, Dominique Giang
Construction Management
As universities experience increasing enrollment and housing shortages, student housing projects require efficient delivery methods capable of meeting strict occupancy deadlines while minimizing disruption to active campus environments. This research investigates the influential drivers behind the utilization of prefabrication on an ongoing student housing project at California Polytechnic State University, San Luis Obispo. The seven-story project consists of 417 student beds and incorporates repetitive layouts and prefabricated building components. Through qualitative interviews with key project stakeholders involved in early decision-making and field execution, the study examines how project objectives, construction constraints, and delivery considerations influenced the decision to implement prefabrication. …
A Framework For Automated Quantity Extraction From .Ifc Models And Normalized Bid Comparison, Asmaa Mohamed Farouk
A Framework For Automated Quantity Extraction From .Ifc Models And Normalized Bid Comparison, Asmaa Mohamed Farouk
Theses and Dissertations
Accurate bid comparison remains a major challenge in construction tendering due to
differences in Bill of Quantities formats, item naming conventions, and pricing methods across
contractor submissions. These variations require time-consuming manual work and often lead to
subjective decisions. This research presents a computational framework that uses Building
Information Modeling through the .ifc file format, combined with text analysis techniques for
automated bid normalization.
The developed system uses a dual-component approach. Phase 1 extracts quantities from .ifc
model files using 3D geometric calculations, automated element classification. Phase 2 uses text
mining with a domain-specific dictionary of construction terms to interpret …
Dual-Polymer Concrete With Recycled Pet And Hdpe Aggregates: Mechanical And Durability Performance Assessment, Omar Abd El Mageed Hammouda
Dual-Polymer Concrete With Recycled Pet And Hdpe Aggregates: Mechanical And Durability Performance Assessment, Omar Abd El Mageed Hammouda
Theses and Dissertations
This study investigates the fresh, mechanical, durability, thermal, performance as well as preliminary financial aspects of dual-polymer concrete incorporating recycled polyethylene terephthalate (PET) as a partial replacement for fine aggregate and recycled high-density polyethylene (HDPE) as a partial replacement for coarse aggregate, with silica fume as a supplementary cementitious material held constant at 10% by weight of cement. The study responds to the convergence of two pressing challenges in the Egyptian context: the scale of plastic waste mismanagement, which results in widespread informal open burning and environmental contamination, and the high operational energy demand of residential buildings in Egypt's hot …
Functionality Risk Assessment And Risk-Based Management For Critical Infrastructure Systems, Anteneh Zewdu Deriba
Functionality Risk Assessment And Risk-Based Management For Critical Infrastructure Systems, Anteneh Zewdu Deriba
Dissertations and Theses
Critical infrastructure systems such as transportation networks serve as the lifelines of modern society, supporting economic activity and ensuring access to critical services. Their disruption under extreme events can produce consequences extending far beyond physical damage to individual assets. In transportation networks for instance, failures of critical assets such as bridges and tunnels can reduce accessibility of communities, limit freight capabilities for commerce, and increase travel distance and travel time of road users. These consequences associated with the system functionality loss are tied not only to the physical vulnerability and failure of individual assets, but also to the system configuration …
Alternate Method For Determining The Liquid Pressure Recovery Factor, Bryson H. Parker
Alternate Method For Determining The Liquid Pressure Recovery Factor, Bryson H. Parker
All Graduate Reports and Creative Projects, Fall 2023 to Present
The term liquid pressure recovery factor aids in determining the flowrate through valves at the maximum flowrate under choking conditions. The pressure recovery factor can be difficult to determine for certain types of valves based on the industry standard procedure. This report looks at the current industry standard procedure and adds a procedure in the event of the industry standard not being feasible. Often it is not feasible for a testing facility to supply enough flow through the valve to reach the industry standard. The industry standard is to test the valve at the maximum flowrate with a constant upstream …
Water Use And Yield Of Subsurface Drip-Irrigated Forage Crops In Northern Utah, Nishchal Tamang
Water Use And Yield Of Subsurface Drip-Irrigated Forage Crops In Northern Utah, Nishchal Tamang
All Graduate Reports and Creative Projects, Fall 2023 to Present
Growing water scarcity in the Intermountain West has increased interest in improving water use efficiency. Subsurface drip irrigation (SDI) has the potential to reduce evapotranspiration (ET), evaporative losses, surface runoff, and deep percolation. SDI adoption in Utah forage crops is limited, and region-specific information on forage crop water use under SDI is lacking. This study compared ET, yield, and productivity of forage crops between SDI and conventional irrigation methods in northern Utah.
The study was conducted on production fields. Applied irrigation, precipitation, and crop yield were directly measured, while deep percolation was estimated using numerical unsaturated flow modeling. Surface runoff …
City Farm Slo - Market Stand Modernization, Juan Diaz
City Farm Slo - Market Stand Modernization, Juan Diaz
Construction Management
This paper analyzes the design, planning, and buildout of a custom multi-level stand, farm signage, and supporting equipment for the non-profit organization City Farm SLO. The overall goal of the project was to provide the farm with a modernized and functional market space. The project first addressed any owner design requirements, the approval of materials lists, and the assessment of existing conditions. The pre-construction consisted of developed drawings, materials lists, cost estimates, and a project schedule. Aside from setting up requirements, the owner provided specific milestones to be achieved during the construction phase of the project. A major risk found …
Evaluation Of Dredged Sediments As A Partial Replacement For Fine Aggregate In Mortar And Concrete, Ashish Gautam
Evaluation Of Dredged Sediments As A Partial Replacement For Fine Aggregate In Mortar And Concrete, Ashish Gautam
Graduate Theses and Dissertations (2019 - present)
The feasibility of using dredged sediment from the Mobile River as a partial replacement for fine aggregate in mortar and concrete was investigated through a series of laboratory characterization and performance evaluation. Three dredged sediment samples (A, B and C) were initially examined, and Sample A was selected for further evaluation due to its closer resemblance to natural fine aggregate. This sample exhibited lower moisture content, higher sand equivalent values, higher specific gravity, and lower absorption. Mortar mixtures with 0, 10, 20, 30, 50, 75 and 100% dredged sediment replacement showed decreasing workability as the replacement level increased. The 20% …
Expected Structural Damage, Residual Capacity, And Repairability Of Light-Frame Timber Houses, Mikayla Caruthers
Expected Structural Damage, Residual Capacity, And Repairability Of Light-Frame Timber Houses, Mikayla Caruthers
Master's Theses
Earthquake damage in light-frame timber structures is commonly evaluated using peak inter-story drift. However, drift alone does not fully represent the remaining structural capacity of a building or its ability to be repaired following a seismic event. Although expected structural damage, residual capacity, and repairability have each been studied extensively, these concepts are often considered independently, leaving limited guidance that links them within a unified post-earthquake assessment methodology.
This thesis establishes an integrated framework that relates expected structural damage, residual capacity, and repairability for light-frame timber residential structures. Existing literature was synthesized to develop drift-based damage-state classifications, identify methods for …
Synthesis And Characterization Of Carbon Quantum Dots And Gold Nanoparticles For Norovirus Biosensing Applications In Water Systems, Breanne Evans
Synthesis And Characterization Of Carbon Quantum Dots And Gold Nanoparticles For Norovirus Biosensing Applications In Water Systems, Breanne Evans
Master's Theses
Rapid, reliable detection of viral pathogens remains a significant challenge across water-treatment and environmental monitoring systems, including drinking-water, wastewater, water reuse, and environmental surveillance applications. Waterborne viral contamination can pose substantial public-health risks, making early detection essential for protecting water quality and responding quickly to treatment failures or contamination events. Direct potable reuse (DPR) is one particularly demanding example because it requires continuous verification of treatment performance and the broader need for rapid virus monitoring extends across many water-treatment and environmental surveillance applications. Norovirus is a priority target because of its widespread occurrence in wastewater, environmental persistence, and exceptionally low …
A Technoeconomic And Lifecycle Analysis Of Catalytic Non-Thermal Plasma Steam Methane Reforming For Renewable Hydrogen Production, Gustav M. King
A Technoeconomic And Lifecycle Analysis Of Catalytic Non-Thermal Plasma Steam Methane Reforming For Renewable Hydrogen Production, Gustav M. King
Master's Theses
When produced with sufficiently low life-cycle emissions, hydrogen may support low-carbon, dispatchable electricity generation to realize statewide renewable energy regulatory objectives. This thesis develops a screening-level electrothermochemical process model of catalytic non-thermal plasma steam methane reforming (CNTP-SMR) and integrates it with techno-economic and life-cycle assessments. Biogas and natural gas fed CNTP-SMR scenarios are compared with conventional steam methane reforming to evaluate hydrogen production cost, global warming potential, and the effects of key operating parameters. Under the baseline assumptions, CNTP-SMR generated hydrogen at a higher levelized cost (baseline of $3.86/kg H2 and a range of $3.68 to $4.11) when compared …
The Role Of Roughness And Geometry In 2d Depth-Averaged Models: A Comparative Study Using Laboratory Flume Experiments, Adrienne Luymes Mckell
The Role Of Roughness And Geometry In 2d Depth-Averaged Models: A Comparative Study Using Laboratory Flume Experiments, Adrienne Luymes Mckell
All Graduate Theses and Dissertations, Fall 2023 to Present
Hydraulic models are commonly used to predict how water moves through rivers and streams, helping engineers manage flood risk and design infrastructure such as bridges, culverts, and restoration projects. One challenge in these models is representing the effects of riverbed roughness, including features such as rocks, vegetation, and other obstacles that slow water flow.
This study investigated whether it is better to represent these roughness features in detail or to simplify them while still accounting for their effects. Laboratory measurements from a controlled channel experiment were used to create two computer models: one that included the detailed shape of the …
Machine Learning For Predictive Energy And Emissions Modeling Of Vehicles And Power Grids In The United States, S M Tanvir Faysal Alam Chowdhoury
Machine Learning For Predictive Energy And Emissions Modeling Of Vehicles And Power Grids In The United States, S M Tanvir Faysal Alam Chowdhoury
Dissertations
The environmental benefits of electric vehicle (EV) adoption depend on more than replacing internal combustion engine vehicles with electric powertrains. EV adoption reshapes electricity demand, interacts with regional generation mixes, and influences travel behavior and congestion, creating a coupled transportation-energy system in which vehicle and power-plant emissions must be evaluated together. This dissertation develops machine-learning frameworks for predicting energy consumption and emissions from vehicles and power grids under rising EV adoption. The first component forecasts grid emissions from EV charging. Using simulation data from NREL's Cambium database, a Prophet-based time-series framework predicts carbon dioxide, nitrous oxide, and methane emission rates …
Strain Monitoring And Site Characterization At Birds Landing From Transmission Tower Implosions Recorded Using Das And Nodal Stations, Prajwal Panthi
Strain Monitoring And Site Characterization At Birds Landing From Transmission Tower Implosions Recorded Using Das And Nodal Stations, Prajwal Panthi
All Graduate Theses and Dissertations, Fall 2023 to Present
When two old electrical transmission towers in the Sacramento River Delta in California were demolished by Pacific Gas and Electric Company, Utah State University saw an opportunity hiding inside the demolition. Because one of the towers stood close to the Sacramento River and its protective levee, they used the controlled collapse not only to monitor strains in the levee and surrounding ground, but also to learn what lied beneath the ground surface.
To capture this event, the researchers buried a 1.4-kilometer fiber optic cable along the ground and connected it to a system called Distributed Acoustic Sensing (DAS), which can …
A Multi-Label Computer Vision-Based Approach For Condition Assessment Of Traffic Signs: Database Compilation, Model Training, And Evaluation, Shailaja Ratna Pandey
A Multi-Label Computer Vision-Based Approach For Condition Assessment Of Traffic Signs: Database Compilation, Model Training, And Evaluation, Shailaja Ratna Pandey
All Graduate Theses and Dissertations, Fall 2023 to Present
Traffic signs help drivers and pedestrians to understand roadway rules, warnings, and directions, which determine how road users should behave. When signs fade or delaminate, they become harder to read and interpret, which creates increased safety hazards. Transportation agencies usually deploy engineers to the field to inspect signs because they need to evaluate and document the sign’s condition based on established standards and engineering judgment. This process requires substantial time and cost and becomes challenging to apply across large highway networks.
This study evaluated two computer vision (CV) models for automated traffic sign condition assessment using highway images collected across …
Evaluating Data Management Practices And Ontology Development For Transportation Safety Assets, Brig Dean Adams
Evaluating Data Management Practices And Ontology Development For Transportation Safety Assets, Brig Dean Adams
All Graduate Theses and Dissertations, Fall 2023 to Present
Data has begun to permeate transportation agencies, with every process, asset, and event generating a great volume of data. This data helps engineers and decision makers determine maintenance schedules, devise infrastructure investment strategies, improve safety, and plan for future needs. Due to the size of transportation agencies and the diversity of the data they collect, their data is often stored in different systems, managed by different groups, and described using inconsistent terminology. These challenges make it difficult to locate information, combine data from multiple sources, and confidently use data to support important decisions.
This research examined how transportation safety asset …
Comparison Of Forces Generated By An Impinging Water Jet Using Numerical And Physical Experimentation, Spencer R. Young
Comparison Of Forces Generated By An Impinging Water Jet Using Numerical And Physical Experimentation, Spencer R. Young
All Graduate Theses and Dissertations, Fall 2023 to Present
When water is released from hydraulic structures such as dams water jets are formed and land on the downstream surfaces. When these jets land there is high potential for erosion or scour. Scour has the potential to compromise the structure and cause the uncontrolled release of water that can endanger downstream communities and infrastructure.
Engineers use tools including computational fluid dynamics (CFD) to inform their designs to mitigate the risk of scour. This study compares how well CFD is able to model the forces created by a water jet by comparing the results to laboratory collected physical data. To make …
A Laboratory Investigation Of Energy Dissipators At Embankment Dams, Nathan L. Young
A Laboratory Investigation Of Energy Dissipators At Embankment Dams, Nathan L. Young
All Graduate Theses and Dissertations, Fall 2023 to Present
Dams play a critical role in protecting communities from flooding and providing reliable water supplies. When large amounts of water pass through a dam’s spillway during floods, the discharge can travel at high speeds and carry potentially destructive kinetic energy that can damage the dam or erode downstream channels if that energy is not properly controlled. To reduce this risk, engineers commonly use hydraulic structures called energy dissipators that slow spillway discharge and safely release it downstream.
Commonly used energy dissipators at embankment dams include stilling basins that rely on a hydraulic jump – a sudden rise in water depth …
Experimental And Numerical Investigation Of Flow Coefficients In Three-Way, T-Port, Ball Valve, Tyler A. Mindrum
Experimental And Numerical Investigation Of Flow Coefficients In Three-Way, T-Port, Ball Valve, Tyler A. Mindrum
All Graduate Theses and Dissertations, Fall 2023 to Present
Many systems that move fluids, like water distribution networks and heating, ventilation, and air conditioning (HVAC) systems, rely on valves to control flow. This study evaluates a three-way valve, which can direct flow in multiple directions, but has not been extensively researched. Testing included physical data collection and computer simulations to compare how the valves behaved under different valve openings and flow conditions. The results showed that computer simulations can predict the general flow behavior of three-way valves, but that physical testing is still important, especially for valves with complex internal geometries.
The Influence Of Rainfall And Land Cover Change On Streamflow Variability In Eastern Florida Watersheds, Brynn Romberger
The Influence Of Rainfall And Land Cover Change On Streamflow Variability In Eastern Florida Watersheds, Brynn Romberger
Theses and Dissertations
Streamflow in Florida watersheds is influenced by both land cover change and rainfall variability, but separating their contributions is difficult because both have changed over recent decades. This study evaluated rainfall trends and land cover change to determine which better explains flood and drought threshold exceedance across 27 USGS streamflow gauges within the St. Johns River Water Management District (SJRWMD) from 1985 to 2025. Streamflow was characterized using change-point, trend, and variability analyses with a six-threshold exceedance framework. Land cover was quantified using the National Land Cover Database (NLCD), rainfall characteristics were derived from the Analysis of Records for Calibration …
Analysis Of Flood Modeling For The Replication Of An Extreme Rainfall Event In South Florida, Lusnel Fritznel Ferdinand
Analysis Of Flood Modeling For The Replication Of An Extreme Rainfall Event In South Florida, Lusnel Fritznel Ferdinand
Electronic Theses and Dissertations
Flooding is the most common natural disaster in the United States, and trends show an increase in flood risk for the foreseeable future. Florida averages 45 flood disasters per year, at an annual cost of $86 million. Communities in Florida are susceptible to a variety of flood-inducing sources, including tropical cyclones, extreme rainfall, and rising sea levels, which can impact the tourism industry and the economic well-being of residents. The first objective of the thesis was to use flood modeling to replicate the extreme rainfall event that caused heavy flooding in Broward County, Florida, from April 12-13, 2023. A commercial …
Comparative Performance Evaluation Of A Gpu-Accelerated Watershed Model For Flood Inundation Mapping, Maliha Ali
Comparative Performance Evaluation Of A Gpu-Accelerated Watershed Model For Flood Inundation Mapping, Maliha Ali
Electronic Theses and Dissertations
This study evaluates PyWMP (Python-based Watershed Modeling and Planning), which is a flood modeling framework developed by the Center for Water Resiliency and Risk Reduction (CWR3) at Florida Atlantic University (FAU). The framework includes a 2D Rain-on-Mesh (ROM) solver, which is an explicit finite-volume shallow-water equation model. PyWMP is compared against HEC-RAS 2D and Flood Modeller for their rain-on-grid capabilities for watershed-scale flood inundation mapping under three design storm scenarios. The performance of PyWMP was evaluated using both quantitative and spatial metrics. Mean absolute error values showed that the average depth difference between each model is under 1 foot. Percent …
Effect Of Geothermal Conditions On Silica Flour Modified Wellbore Cement, Travis Benjamin Broadhurst
Effect Of Geothermal Conditions On Silica Flour Modified Wellbore Cement, Travis Benjamin Broadhurst
Civil Engineering ETDs
Considerable work has been conducted on modified wellbore cements, but a knowledge gap exists in the effects of downhole conditions on cement, especially tensile strength. In this study, a pressure vessel simulates representative downhole conditions (2000C, 1.5 MPa) with a reference solution for geothermal brine and naturally occurring geothermal gases. Samples are removed after 28-days for unconfined compressive strength testing, Brazilian tensile strength testing, elastic moduli measurement, and chemical analyses by X-ray diffraction and electron microprobe. Aqueous chemical analysis suggests multiple active reactions between the geothermal fluid and cement that are exacerbated by higher temperatures, but do not …
Mechanisms Of Advanced And Energy Efficient Wastewater Treatment Technologies, Derek Belka
Mechanisms Of Advanced And Energy Efficient Wastewater Treatment Technologies, Derek Belka
Civil Engineering ETDs
This dissertation combines meta-omics, microbial ecology, and hydrodynamic analysis to explore mechanisms of enhanced biological phosphorus removal (EBPR) and aerobic granular sludge (AGS) in municipal wastewater treatment. Metagenomic and functional gene profiling of a laboratory sequencing batch reactor fed with amino acids and proteins, and with a low abundance of known polyphosphate-accumulating organisms (PAOs), revealed abundant Herpetosiphonaceae and Dermatophilaceae families possessed key PAO metabolic traits, including phosphate scavenging, anaerobic carbon pathways, and ppk2 gene variants aligned with established PAOs. These findings suggest amino acid-driven EBPR can occur via previously unrecognized taxa, challenging current engineering assumptions and highlighting the need for …