Civil and Environmental Engineering Commons^™

Applications Of Stretching Technique And Time Window Effects On Ultrasonic Velocity Monitoring In Concrete, Bibo Zhong, Jinying Zhu

Department of Civil and Environmental Engineering: Faculty Publications

Coda wave interferometry (CWI) has been used to measure the relative wave-velocity change (dV/V) caused by small changes in materials. This study uses the stretching processing technique which has been used for CWI analysis to investigate velocity changes of direct longitudinal (P) wave, direct shear (S) wave, and coda wave in concrete by choosing different time windows of ultrasonic signals. It is found that the obtained wave-velocity change depends on the time window position, because the relative contribution of P wave and S wave is different in each signal window. This paper presents three experimental scenarios of velocity …

A Greenhouse Gas Emissions Inventory For Nebraska: Livestock And Coal Loom Large, Eric R. Holley, Adam Liska

Adam Liska Papers

Mitigation of climate change requires the systematic identification and cataloging of emissions sources at city, state, and national levels. In this study, an inventory of annual greenhouse gas emissions from the state of Nebraska was created based on industry data, and emissions inventories were completed each year from 1990 to 2016. Nebraska’s net emissions were found to increase from 56.2 million metric tons of carbon dioxide equivalents (MMtCO₂e) in 1990 to 87.4 MMtCO₂e in 2016. Agriculture was found to be the sector with the most emissions (36 MMtCO₂e) followed by electricity generation (21 MMtCO …

Ferrihydrite Enrichment In The Rhizosphere Of Unsaturated Soil Improves Nutrient Retention While Limiting Arsenic And Uranium Plant Uptake, Arindam Malakar, Daniel D. Snow, Michael Kaiser, Jordan Shields, Bijesh Maharjan, Harkamal Walia, Daran Rudnick, Chittaranjan Ray

Nebraska Water Center: Faculty Publications

Improvement of nutrient use efficiency and limiting trace elements such as arsenic and uranium bioavailability is critical for sustainable agriculture and food safety. Arsenic and uranium possess different properties and mobility in soils, which complicates the effort to reduce their uptake by plants. Here, we postulate that unsaturated soil amended with ferrihydrite nanominerals leads to improved nutrient retention and helps reduce uptake of these geogenic contaminants. Unsaturated soil is primarily oxic and can provide a stable environment for ferrihydrite nanominerals. To demonstrate the utility of ferrihydrite soil amendment, maize was grown in an unsaturated agricultural soil that is known to …

Nebraska Water Center Annual Report 2022, Nebraska Water Center, Daugherty Water For Food Global Institute

Nebraska Water Center: Administrative Materials

CONTENTS

Overview: The Nebraska Water Center (https://watercenter.unl.edu)

Fast Facts: NWC 2022 Snapshot

Our Work

Water Sciences Lab Sees Rapid Growth in 2022 (https://watercenter.unl.edu/water-sciences-laboratory2)

WSL is Key to AltEn Contamination Monitoring (https://go.unl.edu/mead)

Know Your Well Project Enters Third Phase (https://knowyourwell.unl.edu)

Computer Science Students Build New Platform for Know Your Well Data Collection

Water Sciences Laboratory Helps Nebraska Tackle Nitrate (https://watercenter.unl.edu/water-sciences-laboratory2)

Nebraska Rural Poll Includes Water Quality Questions (https://ruralpoll.unl.edu and https://go.unl.edu/nitrate_strategy)

NWC Holds Platte River Basin Conference (https://watercenter.unl.edu/2022-nebraska-water-conference)

Spring Seminar Series (https://watercenter.unl.edu/spring-seminar-series)

Water Center Undergoes Five-Year …

Accessing 3d Data, Francesca Albrezzi, John Bonnett, Tassie Gniady, Heather Richards-Rissetto, Lisa Snyder

Department of Anthropology: Faculty Publications

The issue of access and discoverability is not simply a matter of permissions and availability. To identify, locate, retrieve, and reuse 3D materials requires consideration of a multiplicity of content types, as well as community and financial investment to resolve challenges related to usability, interoperability, sustainability, and equity. This chapter will cover modes, audiences, assets and decision points, technology requirements, and limitations impacting access, as well as providing recommendations for next steps.

Maize Response To Coupled Irrigation And Nitrogen Fertilization Under Center Pivot, Subsurface Drip And Surface (Furrow) Irrigation: Soil-Water Dynamics And Crop Evapotranspiration, Ali T. Mohammed, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Determination of crop evapotranspiration (ETc) and root zone soil-water dynamics/distribution coupled with nitrogen (N) management strategies is important for effective management of agricultural fields for enhancing production efficiency. However, limited data and knowledge exist that sufficiently inform how soil-water dynamics and ETc response may vary with coupled irrigation levels and different N application timings under different irrigation methods. Extensive field experiments were conducted in 2016 and 2017 under center pivot (CP), subsurface drip irrigation (SDI), and furrow irrigation (FI) at full irrigation treatment (FIT), 80% FIT, 60% FIT, and rainfed treatment (RFT) with N application timing treatments of traditional N …

Exploration Of The Characteristics And Trends Of Electric Vehicle Crashes: A Case Study In Norway, Chenhui Liu, Li Zhao, Chaoru Lu

Mid-America Transportation Center: Final Reports and Technical Briefs

With the rapid growth of electric vehicles (EVs) in the past decade, many new traffic safety challenges are also emerging. With the crash data of Norway from 2011 to 2018, this study gives an overview of the status quo of EV crashes. In the survey period, the proportion of EV crashes in total traffic crashes had risen from zero to 3.11% in Norway. However, in terms of severity, EV crashes do not show statistically significant differences from the Internal Combustion Engine Vehicle (ICEV) crashes. Compared to ICEV crashes, the occurrence of EV crashes features on weekday peak hours, urban areas, …

North 14th Street Corridor Improvements Project, Ashley Nguyen, Samantha Corey, Maricela Paramo Reyes, Pooja Rajeev

Honors Program: Senior Projects (Public)

The following project report was a study of the North 14^th Street corridor ranging from Adams Street to Virginia Street conducted by University of Nebraska-Lincoln students for a senior design capstone project. Main focuses of the project were the intersection of Adams and 14^th Street and the 14^th Street bridges over Cornhusker Highway and Oak Creek. Study of this area included work in transportation, traffic, structural, geotechnical, water resources, and environmental engineering. However, this report done by students should not take the place of work done by licensed professional engineers.

This project was done in collaboration with …

Assessing The Impacts Of Covid-19 On Activity-Travel Scheduling: A Survey In The Greater Toronto Area, Alireza Dianat, Jason Hawkins, Khandker Nurul Habib

Department of Civil and Environmental Engineering: Faculty Publications

The COVID-19 lockdown provided many individuals an opportunity to explore changes in their daily routines, particularly when considered in combination with an ever-changing Information and Communication Technology (ICT) landscape. These new routines and alternative activities have the potential to be continued in the post-COVID era. Transportation planners must understand how routines vary to effectively estimate activity-travel scheduling. The purpose of this study is to determine the influence of the COVID-19 pandemic lockdown on activity-travel behavior and the adoption of ICT-based alternative options. A special emphasis is placed on predicting the long-term effects of this disturbance on activity-travel scheduling. This study …

Effects Of Friction On The Dynamic Behavior Of Flexible Rocking Bodies, S. Mohammadreza Farooghi Mehr, Christine E. Wittich

Department of Civil and Environmental Engineering: Faculty Publications

Flexible rocking bodies are freestanding structures that are free to deform and rock, potentially overturning, when subject to ground excitation. Prior research on the seismic behavior of this type of structure typically assumed sufficient friction at the ground surface to avoid sliding motion. However, experimental observations showed that this assumption was often violated and structures demonstrated non-negligible sliding during their responses. The overarching goal of this study is to evaluate the impact of sliding on the overturning response of flexible rocking bodies. To this end, a two-dimensional analytical model was developed in a Lagrangian formulation, which is presented in this …

Resistome And Mobilome In Surface Runoff From Manured Soil As Affected By Setback Distance, Noelle Mware, Maria C. Hall, Selvakumar Rajendran, John E. Gilley, Amy Schmidt, Shannon Bartelt-Hunt, Yifan Zhang, Xu Li

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Land application of livestock manure introduces antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) into the soil environment. The objectives of this study were to examine the changes of resistome and mobilome in runoff and soil as a function of setback distance, i.e., the distance between manured soil and surface water, and to quantify the contributions of manure and background soil to the ARGs and MGEs in surface runoff. The resistome and mobilome in runoff and soil from a field-scale plot study were characterized using a high throughput quantitative polymerase chain reaction (HT-qPCR) array. It was estimated that a …

Emission Of Volatile Organic Compounds From Land-Applied Beef Cattle Manure As Affected By Application Method, Diet, And Soil Water Condition, Bryan L. Woodbury, John E. Gilley, David B. Parker, David B. Marx

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Land application of beef cattle manure may result in the emission of volatile organic compounds (VOC). This study was conducted to evaluate the effects of diet, land application method, soil water condition, and time since manure application on VOC emissions. Manure was collected from feedlot pens where cattle were fed diets containing 0%, 10%, or 30% wet distillers grains with solubles (WDGS). The effects of manure application method (surface-applied or incorporated) and soil water condition (saturated or wet) on VOC emissions were measured over a 48 h period. Heptanoic, hexanoic, isobutyric, and isovaleric acids contributed 23.5%, 17.6%, 9.26%, and 3.39% …

Geospatial Synthesis Of Biogeochemical Attributions Of Porphyrins To Oil Pollution In Marine Sediments Of The Gulf Of México, Francisco Muñoz-Arriola, José Vinicio Macías-Zamora

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Porphyrins are highly persistent in the environment and represent a helpful biogeochemical attribute to assess the spatial distribution of the effects of oil spills on ecosystems and their resilience. In areas prone to natural and human-originated oil spills, the measurement of VO– and nickel– porphyrins in marine–sediment samples can identify the effects of oil pollution across spatiotemporal scales. The goal is to explore whether or not these compounds can be useful indicators of the geospatial attributions of oil contamination in the surficial sediments. We hypothesize that the geospatial gradients of porphyrins in marine sediments from petroleum spills and seepage activities—related …

The Brazilian Soil Spectral Service (Braspecs): A User-Friendly System For Global Soil Spectra Communication, José A. M. Demattê, Ariane Francine Da Silveira Paiva, Raul Roberto Poppiel, Nícolas Augusto Rosin, Luis Fernando Chimelo Ruiz, Fellipe Alcantara De Oliveira Mello, Budiman Minasny, Sabine Grunwald, Yufeng Ge, Eyal Ben Dor, Asa Gholizadeh, Cecile Gomez, Sabine Chabrillat, Nicolas Francos, Shamsollah Ayoubi, Dian Fiantis, James Kobina Mensah Biney, Changkun Wang, Abdelaziz Belal, Salman Naimi, Najmeh Asgari Hafshejani, Henrique Bellinaso, Jean Michel Moura-Bueno, Nélida E. Q. Silvero

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Although many Soil Spectral Libraries (SSLs) have been created globally, these libraries still have not been operationalized for end-users. To address this limitation, this study created an online Brazilian Soil Spectral Service (BraSpecS). The system was based on the Brazilian Soil Spectral Library (BSSL) with samples collected in the Visible–Near–Short-wave infrared (vis–NIR–SWIR) and Midinfrared (MIR) ranges. The interactive platform allows users to find spectra, act as custodians of the data, and estimate several soil properties and classification. The system was tested by 500 Brazilian and 65 international users. Users accessed the platform (besbbr.com.br), uploaded their spectra, and received soil organic …

Unmanned Aerial System-Based Data Ferrying Over A Sensor Node Station Network In Maize, Jasreman Singh, Yufeng Ge, Derek M. Heeren, Elizabeth Walter-Shea, Christopher M. U. Neale, Suat Irmak, Mitchell S. Maguire

Department of Agricultural and Biological Systems Engineering: Faculty Publications

© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).

High Throughput Analysis Of Leaf Chlorophyll Content In Sorghum Using Rgb, Hyperspectral, And Fluorescence Imaging And Sensor Fusion, Huichun Zhang, Yufeng Ge, Xinyan Xie, Abbas Atefi, Nuwan Wijewardane,, Suresh Thapa

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background: Leaf chlorophyll content plays an important role in indicating plant stresses and nutrient status. Traditional approaches for the quantification of chlorophyll content mainly include acetone ethanol extraction, spectrophotometry and high-performance liquid chromatography. Such destructive methods based on laboratory procedures are time consuming, expensive, and not suitable for high-throughput analysis. High throughput imaging techniques are now widely used for non-destructive analysis of plant phenotypic traits. In this study three imaging modules (RGB, hyperspectral, and fluorescence imaging) were, separately and in combination, used to estimate chlorophyll content of sorghum plants in a greenhouse environment. Color features, spectral indices, and chlorophyll fluorescence …

Maize Response To Irrigation And Nitrogen Under Center Pivot, Subsurface Drip And Furrow Irrigation: Water Productivity, Basal Evapotranspiration And Yield Response Factors, Ali T. Mohammed, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Information and data about quantification and comparison of crop water productivity indices for various irrigation levels and methods and nitrogen (N) application timings “simultaneously” under the same conditions do not exist. Unprecedented and extensive field experiments were conducted for maize (Zea mays L.) in 2016 and 2017 under center pivot (CP), subsurface drip irrigation (SDI) and furrow irrigation (FI) methods with full irrigation treatment (FIT), 80% FIT, 60% FIT and rainfed treatment (RFT) with three N application timings. N treatments were: (i) traditional (TN), (ii) non-traditional-1 (NT-1) and (iii) non-traditional-2 (NT-2). Irrigation yield production functions (IYPF); evapotranspiration-yield production functions …

Sources Of Variability And Uncertainty In Food-Energy-Water Nexus Systems, Heydi Calderon-Ambelis, Deepak R. Keshwani

Department of Agricultural and Biological Systems Engineering: Faculty Publications

A nexus approach contributes to the strategic allocation of resources to secure food, energy, and water for the world population. Integrated models considering the complex interactions across food, energy, and water (FEW) enhance decision-making and strategic planning towards resilience. However, a significant number of the existing integrated models leave unaddressed the inherent variability and uncertainty present in the FEW sectors. Here, we review the importance of characterizing variability over spatial and temporal scales and the importance of decreasing the uncertainty present within a FEW nexus systems. The review also discusses existing modeling tools that address variability and uncertainty on single …

Modeling Water Quantity And Quality Nonlinearities For Watershed Adaptability To Hydroclimate Extremes In Agricultural Landscapes, Juan Carlos Jaimes-Correa, Francisco Muñoz-Arriola, Shannon Bartelt-Hunt

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Changing water supplies and demands, inherent to climate fluctuations and human activities, are pushing for a paradigm shift in water management worldwide. The occurrence of extreme hydrometeorological and climate events such as extended wet periods and droughts, compounded with contaminants, impair the access to water resources, demanding novel designs, construction, and management across multiple hydrologic scales and biogeochemical processes. A constraint to studying hydrologic and biogeochemical disturbances and improving best management practices for water quantity and quality at the watershed scale resides in the suitable monitoring, data availability, and the creation of frameworks. We hypothesize that streamflow and contaminants, simulated …

Comparison Effect On Biogas Production From Vegetable And Fruit Waste With Rumen Digesta Through Co-Digestion Process, Anika Tasnim, Muhammad Rashed Al Mamun, Md Anwar Hossen, Md Towfiqur Rahman, Md Janibul Alam Soeb

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Biogas is the best renewable energy as it can be produced from any biomass for example any plant or living organism. The purpose of this research was to produce biomethane from co-digestion of vegetable and fruit waste with rumen digesta through anaerobic digestion process. In this research, two trials of experiment were conducted. Each trial has three different sample with different mixing ratios. Raw materials used in the experiment was rumen digesta of goat and cow, potato, capsicum, cucumbers, onions, radish, cauliflower, carrot, leafy vegetables, apple, banana, and papaya. In each sample, 1200 gram of raw materials were used. Hydraulic …

Influences Of Artificial Light On Mating Of Black Soldier Fly (Hermetia Illucens): A Review, Md Rabiul Awal, Md Masudur Rahman, Md Abdur Razzak Choudhury, Md Mehedi Hasan, Md Towfiqur Rahman, Md Fuad Mondal

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Black soldier fly (Hermetia illucens) is a potential insect species which can convert biodegradable materials and some indigestible organic waste into valuable biomass. Because of having good quality of fat and protein, its production and use in animal feed are being extended day by day. To fulfill the future demand re-searchers are trying to find out the successful mass rearing techniques of H. illucens in laboratory or indoor condition. However, the most critical part of H. illucens mass production is obtaining successful mating. This insect is very sensitive to light. It prefers direct sunlight for its …

Geospatial Distribution Of Age-Adjusted Incidence Of The Three Major Types Of Pediatric Cancers And Waterborne Agrichemicals In Nebraska, Balkissa S. Ouattara, Jagadeesh Puvvula, Azar Abadi, Siddhi Munde, Alan S. Kolok, Shannon Bartelt-Hunt, Jesse E. Bell, Christopher S. Wichman, Eleanor Rogan

Department of Civil and Environmental Engineering: Faculty Publications

This study was conducted to examine, at the county level, the relationship between pediatric cancer incidence rate and atrazine and nitrate mean concentrations in surface and groundwater. A negative binomial regression analysis was performed to investigate the association between central nervous system (CNS) tumors, leukemia, lymphoma, and atrazine and nitrate mean concentrations in groundwater. The age-adjusted brain and other CNS cancer incidence was higher than the national average in 63% of the Nebraska counties. After controlling for the counties socio-economic status and nitrate concentrations in groundwater, counties with groundwater atrazine concentrations above 0.0002 μg/L had a higher incidence rate for …

Probability-Based Crack Width Estimation Model For Flexural Members Of Underground Rc Box Culverts, Sang-Hyo Kim, Syed Haider Ali Shah, Sang-Kyun Woo, Inyeop Chu, Chungwook Sim

Department of Civil and Environmental Engineering: Faculty Publications

Crack control for slabs and beams in current design practices in Korea are based on the Frosch’s model, which is adapted in ACI 318. It is more difficult to have consistent quality control in underground construction sites, such as the RC box culverts used for electric power distribution built below the ground level. There are more discrepancies between the as-built dimensions and the design dimensions provided in drawings in these structures. Due to this variability in construction error, the crack widths measured in such structures have higher potential to have more differences than the calculated values. Although crack control is …

Inspiring Young Students To Pursue Engineering, Tami M. Brown-Brandl, Brian Steward

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Imagine elementary school students understanding inputs and outputs, choosing appropriate sensors, and programming an autonomous robot. Imagine middle school students learning about PID control and creating advanced programs for sensor-based decision making. Imagine high school students using CAD to design drive trains, manufacturing parts with CNC machine tools, using finite element analysis to adjust the design, ordering custom components from a machine shop, using image processing for target recognition, and applying path planning and motion control. We have witnessed all of these skills. They happened outside of traditional classrooms, and they were all student-driven.

Yes, it’s true, and it’s amazing. …

Cive 440: Reinforced Concrete Design I: Faculty-Led Inquiry Into Reflective And Scholarly Teaching (First) Course Portfolio, Chungwook Sim

UNL Faculty Course Portfolios

This Faculty-led Inquiry into Reflective and Scholarly Teaching (FIRST) Course Portfolio documents the instructor’s teaching practices and student learning for Reinforced Concrete Design I (CIVE 440) course. The contents of this course portfolio captures the CIVE 440 course that was taught on the Lincoln campus during the semester of Fall 2022. CIVE 440 is a structural engineering design elective offered every year in the Department of Civil and Environmental Engineering on both Lincoln and Omaha campus at University of Nebraska-Lincoln (UNL). The course objective is to help students to be able to use theory and experience to proportion and detail …

Research On High-Rap Mixtures With Rejuvenator - Field Implementation, Nitish R. Bastola, Mahdieh Khedmati, Hamzeh Haghshenas

Nebraska Department of Transportation: Research Reports

The use of Rejuvenating Agents (RAs), as Recycled Asphalt Pavement (RAP) modifiers, has been increasing over the past years. However, the field performance of asphalt mixtures containing high-RAP materials and modified with RAs has raised some concerns regarding the long-term performance of RAs. This study evaluated the laboratory and field performance of high-RAP mixtures with and without bio-oil RA. Three sets of plant-produced specimens were collected: 1) laboratory-compacted; 2) field-compacted and cored after paving; and 3) field-compacted and cored after one and two years. The Hamburg Wheel Tracking (HWT) test was used to evaluate the specimens' resistance to rutting and …

Low-Level Groundwater Atrazine In High Atrazine Usage Nebraska Counties: Likely Effects Of Excessive Groundwater Abstraction, Moses New-Aaron, Olufemi P. Abimbola, Raheleh Mohammadi, Oluwaseun Famojuro, Zaeema Naveed, Azar M Abadi, Jesse E. Bell, Shannon Bartelt-Hunt, Eleanor G. Rogan

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Recent studies observed a correlation between estrogen-related cancers and groundwater atrazine in eastern Nebraska counties. However, the mechanisms of human exposure to atrazine are unclear because low groundwater atrazine concentration was observed in counties with high cancer incidence despite having the highest atrazine usage. We studied groundwater atrazine fate in high atrazine usage Nebraska counties. Data were collected from Quality Assessed Agrichemical Contaminant Nebraska Groundwater, Parameter–Elevation Regressions on Independent Slopes Model (PRISM), and water use databases. Descriptive statistics and cluster analysis were performed. Domestic wells (59%) were the predominant well type. Groundwater atrazine was affected by well depth. Clusters consisting …

Gis-Based Volunteer Cotton Habitat Prediction And Plant-Level Detection With Uav Remote Sensing, Tianyi Wang, Xiaohan Mei, J. Alex Thomasson, Chenghai Yang, Xiongzhe Han, Pappu Kumar Yadav, Yeyin Shi

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Volunteer cotton plants germinate and grow at unwanted locations like transport routes and can serve as hosts for a harmful cotton pests called cotton boll weevils. The main objective of this study was to develop a geographic information system (GIS) framework to efficiently locate volunteer cotton plants in the cotton production regions in southern Texas, thus reducing time and economic cost for their removal. A GIS network analysis tool was applied to estimate the most likely routes for cotton transportation, and a GIS model was created to identify and visualize potential areas of volunteer cotton growth. The GIS model indicated …

Source Apportionment And Health Risk Assessment Of Nitrate In Foothill Aquifers Of Western Ghats, South India, Banajarani Panda, S. Chidambaram, Daniel D. Snow, Arindam Malakar, Dhiraj Kr Singh, L. Ramanathan

Nebraska Water Center: Faculty Publications

The present research reports the level of nitrate (NO₃^-), associated health risks and possible sources of contamination in groundwater from south India. Many samples (32%) are above or approaching the recommended level of NO₃^- for safe drinking water. The correlation analysis indicates different sources of NO₃^- contamination in different regions rather than a common origin. The isotopic measurements provide information about potential nitrogen sources contributing NO₃^- to the groundwater. Based on isotope analysis, the sources of NO₃^- in the groundwater of this region are likely to be from (a) …

Injectable Decellularized Nucleus Pulposus Tissue Exhibits Neuroinhibitory Properties, Logan M. Piening, David J. Lillyman, Fei San Lee, Alvaro Moreno Lozano, Jeremy R. Miles, Rebecca A. Wachs

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background: Chronic low back pain (LBP) is a leading cause of disability, but treatments for LBP are limited. Degeneration of the intervertebral disc due to loss of neuroinhibitory sulfated glycosaminoglycans (sGAGs) allows nerves from dorsal root ganglia to grow into the core of the disc. Treatment with a decellularized tissue hydrogel that contains sGAGs may inhibit nerve growth and prevent discassociated LBP.

Methods: A protocol to decellularize porcine nucleus pulposus (NP) was adapted from previous methods. DNA, sGAG, α-gal antigen, and collagen content were analyzed before and after decellularization. The decellularized tissue was then enzymatically modified to be …